Anti-wrinkle and anti-aging composition and application thereof

Abstract

The invention relates to an anti-wrinkle and anti-aging composition and application thereof. The anti-wrinkle and anti-aging composition is prepared from hydrolyzed rice protein, a bifidus yeast fermentation product lysate, dipeptide diaminobutyryl benzyl amide diacetate, adenosine, hydroxydecyl ubiquinone, ceramide NP, palmitoyl tripeptide-5, carnosine and acetyl hexapeptide-8. According to the present invention, the components achieve the synergistic effect, such that the fibroblast proliferation can be promoted, the cell metabolism can be improved, the generation of collagen, elastin and the like can be promoted, the excellent oxidation resistance can be provided, and the effects of expression line inhibition, existing wrinkle fading and long-term aging resistance can be combined.

Description

TECHNICAL FIELD

[0001] The present application belongs to the technical field of cosmetics, and relates to an anti-wrinkle and anti-aging composition and application thereof. BACKGROUND

[0002] Skin is the largest organ of the human body and an important organ for maintaining human life and health. Due to environmental pollution, life pressure, unhealthy living habits and other factors, skin aging and wrinkle problems are increasingly prominent, and the market demand for anti-wrinkle and anti-aging skin care products is expanding.

[0003] At present, there are many cosmetic products on the market with anti-wrinkle and anti-aging effects, but some anti-wrinkle and anti-aging products have no obvious effect, the propaganda is too exaggerated, the actual use effect does not match the propaganda, and consumers can hardly see obvious anti-wrinkle effect after using for a period of time. At present, general anti-wrinkle and anti-aging products can be divided into non-penetration absorption and penetration absorption. The products of non-penetration absorption are mostly added with some single anti-wrinkle and anti-aging ingredients, without multi-dimensional and systematic anti-wrinkle and anti-aging, so that the use effect is not obvious. The penetration absorption such as injection of botulinum toxin or hyaluronic acid often has reports of serious sequelae of injection of botulinum toxin, and face collapse phenomenon, and injection of hyaluronic acid has certain discomfort and needs to be injected again for maintenance.

[0004] CN105943475A discloses an anti-wrinkle eye cream containing traditional Chinese medicine ingredients, and a preparation method and application thereof. The anti-wrinkle eye cream comprises glycerol, methyl glucose glycoside eicosane oxide, stearic acid, silicon smooth agent, hyaluronic acid, isomerose, rose essential oil, radix astragali extract, acanthopanax senticosus extract, rhodiola extract, gynostemma pentaphyllum extract and purple stem extract. The anti-wrinkle eye cream provided by the application is refreshing and smooth, mild and non-irritating, can maintain the moisture of the eye skin for a long time, promote the blood circulation of the eye, and effectively reduce the production of fine lines of the eye, but cannot repair the already produced fine lines.

[0005] CN118634168A discloses a whitening and anti-aging composition, a whitening and anti-aging cream and a preparation method thereof. The whitening and anti-aging composition comprises glycyrrhizin 0.1-1%, rhamnose silanol 0.1-10%, yarrow extract 0.1-10%, peony root extract 0.1-40%, acetylated sodium hyaluronate 0.1-10%, humectant 20-24% and the balance of water. The components of the composition provided by the application cooperate with each other, fully play their roles, the whitening and anti-aging effects of the composition are remarkable, the skin state and texture are improved, the combination is reasonable, and the whitening, anti-aging and wrinkle removal effects are excellent, but the effect of combining anti-wrinkle and anti-aging from multiple dimensions cannot be improved.

[0006] In summary, in order to truly realize multi-dimensional anti-wrinkle and anti-aging without side effects and free and convenient use, the present application provides an anti-wrinkle and anti-aging composition and application thereof. SUMMARY

[0007] In view of the deficiencies of the prior art, the present application aims to provide an anti-wrinkle and anti-aging composition and its application, which can achieve multi-dimensional anti-wrinkle and anti-aging effects while improving the effects.

[0008] To achieve the object of the present application, the present application adopts the following technical solutions:

[0009] In a first aspect, the present application provides an anti-wrinkle and anti-aging composition, which comprises hydrolyzed rice protein, saccharomyces boulardii ferment lysate, dipeptide diaminobutyroyl hydrazide benzylamide diacetate, adenosine, hydroxydecyl ubiquinone, ceramide NP, palmitoyl tripeptide-5, carnosine and acetyl hexapeptide-8.

[0010] In the present application, the hydrolyzed rice protein contains small molecule peptides, which can promote the proliferation of fibroblasts, and the fibroblasts can synthesize and secrete collagen, elastin, generate collagen fibers, reticular fibers and elastic fibers, thereby enriching the skin; the saccharomyces boulardii ferment lysate contains a variety of beneficial bioactive ingredients, which can promote the regeneration and repair of skin cells and enhance the self-repairing ability of the skin; the dipeptide diaminobutyroyl hydrazide benzylamide diacetate can act on the postsynaptic membrane, bind to the epsilon subunit of nmAChR, thereby blocking the binding of acetylcholine to the receptor, ultimately leading to receptor occlusion, and sodium ions cannot be released in the occluded state, so the muscles relax and the wrinkles are reduced. In the present application, the hydrolyzed rice protein, the saccharomyces boulardii ferment lysate and the dipeptide diaminobutyroyl hydrazide benzylamide diacetate cooperate with each other and have a synergistic effect, which can relax the facial skin, effectively reduce the wrinkles caused by muscle contraction, promote the synthesis of collagen, make the skin more full and firm, and achieve the effects of lightening existing wrinkles and anti-aging.

[0011] In the present application, adenosine provides energy for skin cells, enhances the vitality and metabolic function of cells, can stimulate skin cells to synthesize collagen and elastin, and can inhibit the release of inflammatory mediators, relieve skin inflammation, and help delay skin aging; hydroxydecyl ubiquinone is a nutrient that can improve body function and prevent aging, it promotes skin metabolism and the synthesis of skin ATP, makes the life force of weakened cells stronger, and at the same time is one of the most important antioxidants in the body, can scavenge free radicals and protect the skin from oxidative damage; ceramide NP is an important component of the skin barrier, has deep and long-lasting moisturizing and strong skin repair functions, can improve skin elasticity and delay aging. Adenosine, hydroxydecyl ubiquinone and ceramide NP cooperate with each other and have a synergistic effect, which can provide energy for skin cells, promote the generation of collagen and elastin, and jointly maintain the healthy state of the skin, delay skin aging.

[0012] Palmitoyl tripeptide-5 stimulates collagen synthesis in the skin through tissue growth factor-β (TGF-β), achieving the anti-wrinkle effect of smoothing wrinkles and firming the skin. Palmitoyl tripeptide-5 promotes skin cell growth, inhibits oxygen free radicals and hydroxyl free radicals, promotes the synthesis of matrix proteins, especially collagen, and can also increase the production of elastin, hyaluronic acid, glycosaminoglycans and fibronectin.

[0013] Carnosine has antioxidant properties, which can scavenge free radicals and protect the skin from oxidative damage. At the same time, it can inhibit glycation reactions and reduce the formation of advanced glycation end products, thereby delaying skin aging.

[0014] Acetyl hexapeptide-8 can reduce the depth of wrinkles caused by facial muscle contractions, particularly on the forehead and around the eyes. Acetyl hexapeptide-8 competes with SNAP-25 for a site on the vesicle complex, thus influencing complex formation. If the vesicle complex is slightly unstable, the vesicles cannot effectively release neurotransmitters, leading to weakened muscle contraction and preventing wrinkle formation.

[0015] This invention creatively combines hydrolyzed rice protein, bifida ferment lysate, dipeptide diaminobutyryl benzyl amide diacetate, adenosine, hydroxydecyl ubiquinone, ceramide NP, palmitoyl tripeptide-5, carnosine, and acetyl hexapeptide-8. The synergistic effect of these components can achieve precise anti-aging effects from multiple dimensions, combining the inhibition of expression lines, the fading of existing wrinkles, and long-term anti-aging benefits.

[0016] Preferably, the anti-wrinkle and anti-aging composition comprises, by weight, 1-3 parts hydrolyzed rice protein, 0.5-2 parts Bifida ferment lysate, 1.5-6 parts dipeptide diaminobutyryl benzylamide diacetate, 0.03-0.3 parts adenosine, 0.01-0.3 parts hydroxydecyl ubiquinone, 0.1-1 parts ceramide NP, 0.5-5 parts palmitoyl tripeptide-5, 0.05-0.3 parts carnosine, and 1-10 parts acetyl hexapeptide-8.

[0017] The composition includes 1-3 parts hydrolyzed rice protein (e.g., 1 part, 1.5 parts, 2 parts, 2.5 parts, 3 parts, etc.), 0.5-2 parts Bifida ferment lysate (e.g., 0.5 parts, 0.8 parts, 1.1 parts, 1.4 parts, 1.7 parts, 2 parts, etc.), and 1.5-6 parts dipeptide diaminobutyryl benzylamide diacetate (e.g., 1.5 parts, 2 parts, 2.5 parts, 3 parts, 3.5 parts, 4 parts, 4.5 parts, 5 parts, 5 parts, etc.). 5 parts, 6 parts, etc.; adenosine 0.03~0.3 parts, for example, 0.03 parts, 0.05 parts, 0.1 parts, 0.15 parts, 0.22 parts, 0.25 parts, 0.3 parts, 0.5 parts, 0.9 parts, 1.3 parts, 1.7 parts, 2 parts, etc.; hydroxydecyl ubiquinone 0.01~0.3 parts, for example, 0.01 parts, 0.05 parts, 0.1 parts, 0.15 parts, 0.2 parts, 0.25 parts, 0.3 parts, etc.; ceramide NP 0.1 to 1 part, for example, 0.1 part, 0.3 part, 0.5 part, 0.7 part, 0.9 part, 1 part, etc.; Palmitoyl tripeptide-5 0.5 to 5 parts, for example, 0.5 part, 1 part, 1.5 part, 2 part, 2.5 part, 3 part, 3.5 part, 4 part, 4.5 part, 5 part, etc.; Carnosine 0.05 to 0.3 parts, for example, 0.05 part, 0.1 part, 0.15 part, 0.22 part, 0.25 part, 0.3 part, etc.; Acetyl hexapeptide-8 1 to 10 parts, for example, 1 part, 2 parts, 3 parts, 4 parts, 5 parts, 6 parts, 7 parts, 8 parts, 9 parts, 10 parts, etc.

[0018] Preferably, the mass ratio of hydrolyzed rice protein, Bifida ferment lysate, and dipeptide diaminobutyryl benzyl amide diacetate is (1~3):(0.5~1):(4~6), wherein the specific values ​​in (1~3) can be 1, 1.4, 1.8, 2.2, 2.6, 3, etc.; the specific values ​​in (0.5~1) can be 0.5, 0.6, 0.7, 0.8, 0.9, 1, etc.; and the specific values ​​in (4~6) can be 4, 4.4, 4.8, 5.2, 5.6, 6, etc.

[0019] Preferably, the mass ratio of adenosine, hydroxydecyl ubiquinone, and ceramide NP is (0.1~0.3):(0.01~0.3):(0.1~0.3), wherein the specific values ​​in 0.1~0.3 can be 0.1, 0.13, 0.16, 0.19, 0.22, 0.25, 0.28, 0.3, etc., and the specific values ​​in 0.01~0.3 can be 0.01, 0.05, 0.1, 0.15, 0.2, 0.25, 0.3, etc.

[0020] Furthermore, when the above components are combined in a specific mass ratio, they have a more significant effect in wrinkle removal and anti-aging.

[0021] Preferably, the hydrolyzed rice protein is prepared by a method comprising the following steps:

[0022] (1) Mix rice powder with alkaline solution and then subject it to ultra-high pressure treatment to obtain pretreated solution.

[0023] (2) The pretreatment solution is mixed with cellulase and saccharifying enzyme for enzymatic hydrolysis, solid-liquid separation is performed to obtain solid product, and then the solid product is dispersed in water to obtain mixture I.

[0024] (3) Mix the mixture I obtained in step (2) with trypsin for enzymatic hydrolysis, separate the solid and liquid to obtain a solid product, and then disperse the solid product in water to obtain mixture II.

[0025] (4) Mix the mixture II obtained in step (3) with papain and / or neutral protease for enzymatic hydrolysis to obtain mixture III.

[0026] (5) The mixture III obtained in step (4) is subjected to ultrafiltration, the filtrate is concentrated and then dried to obtain the hydrolyzed rice protein.

[0027] In step (1) of this invention, rice powder is mixed with alkaline solution and then subjected to ultra-high pressure treatment. The ultra-high pressure treatment changes the cell structure of rice, which is beneficial to the full contact between enzyme and substrate in the subsequent enzymatic hydrolysis process.

[0028] In step (2) of this invention, cellulase can decompose the cellulose in rice, and saccharifying enzymes may convert sugars such as starch into smaller molecules that are easier to process, thereby reducing the interference of these components on protein extraction; in step (3), trypsin is added for enzymatic hydrolysis to decompose the trypsin in rice, thereby reducing the irritation and corrosiveness of trypsin to the skin; in step (4), papain and / or neutral protease are added for enzymatic hydrolysis, which can refine the protein structure and release more active peptides. The structure of rice protein is relatively complex. This invention uses the sequential action of different enzymes in the three-step enzymatic hydrolysis method to gradually decompose the complex structure, thereby more effectively obtaining small molecule peptides that can promote fibroblast proliferation.

[0029] Preferably, the alkaline solution in step (1) includes an aqueous solution of NaOH or an aqueous solution of NaHCO3.

[0030] Preferably, the mixing temperature in step (1) is 20~40℃ (e.g., 20℃, 25℃, 30℃, 35℃, 40℃, etc.), and the mixing time is 2~4h (e.g., 2h, 2.5h, 3h, 3.5h, 4h, etc.).

[0031] Preferably, the mixing is carried out by stirring.

[0032] Preferably, the pressure of the ultra-high pressure treatment in step (1) is 300~500MPa (for example, it can be 300MPa, 350MPa, 400MPa, 450MPa, 500MPa, etc.), and the ultra-high pressure treatment time is 5~10min (for example, it can be 5min, 6min, 7min, 8min, 9min, 10min, etc.).

[0033] Preferably, the temperature of enzymatic hydrolysis in step (2) is 70~90℃ (e.g., 70℃, 75℃, 80℃, 85℃, 90℃, etc.), and the time of enzymatic hydrolysis is 1~4h (e.g., 1h, 1.5h, 2h, 2.5h, 3h, 3.5h, 4h, etc.).

[0034] Preferably, the enzymatic hydrolysis in step (2) is carried out under conditions of pH 7 to 10 (for example, the pH value can be 7, 7.5, 8, 8.5, 9, 9.5, 10, etc.).

[0035] Preferably, the amount of cellulase added to the pretreatment solution is 40-70 U / g (e.g., 40 U / g, 45 U / g, 50 U / g, 55 U / g, 60 U / g, 65 U / g, 70 U / g, etc.), and the amount of saccharifying enzyme added to the pretreatment solution is 30-60 U / g (e.g., 30 U / g, 35 U / g, 40 U / g, 45 U / g, 50 U / g, 55 U / g, 60 U / g, etc.).

[0036] Preferably, the temperature of enzymatic hydrolysis in step (3) is 55~70℃ (e.g., 55℃, 60℃, 65℃, 70℃, etc.), and the time of enzymatic hydrolysis is 1~4h (e.g., 1h, 1.5h, 2h, 2.5h, 3h, 3.5h, 4h, etc.).

[0037] Preferably, the enzymatic hydrolysis in step (3) is carried out under conditions of pH 7 to 10 (for example, the pH value can be 7, 7.5, 8, 8.5, 9, 9.5, 10, etc.).

[0038] Preferably, the amount of trypsin added to the mixture I is 60~90 U / g (for example, it can be 60 U / g, 65 U / g, 70 U / g, 75 U / g, 80 U / g, 85 U / g, 90 U / g, etc.).

[0039] Preferably, step (4) involves mixing the mixture II obtained in step (3) with papain and neutral protease for enzymatic hydrolysis to obtain mixture III.

[0040] Preferably, the amount of papain added to the mixture II is 40-60 U / g (e.g., 40 U / g, 45 U / g, 50 U / g, 55 U / g, 60 U / g, etc.), and the amount of neutral protease added to the mixture II is 10-30 U / g (e.g., 10 U / g, 15 U / g, 20 U / g, 25 U / g, 30 U / g, etc.).

[0041] Preferably, the temperature of enzymatic hydrolysis in step (4) is 30~60℃ (e.g., 30℃, 35℃, 40℃, 45℃, 50℃, 55℃, 60℃, etc.), and the time of enzymatic hydrolysis is 1~4h (e.g., 1h, 1.5h, 2h, 2.5h, 3h, 3.5h, 4h, etc.).

[0042] Preferably, the enzymatic hydrolysis in step (4) is carried out under conditions of pH 5 to 7 (for example, pH can be 7, 7.5, 8, 8.5, 9, 9.5, 10, etc.).

[0043] Preferably, the ultrafiltration membrane in step (5) has a molecular weight cutoff of 2 to 4 kDa (for example, it can be 2 kDa, 2.5 kDa, 3 kDa, 3.5 kDa, 4 kDa, etc.).

[0044] In a second aspect, the present invention provides the application of the anti-wrinkle and anti-aging composition as described in the first aspect in cosmetics.

[0045] This composition can be used in a variety of cosmetics, including serums, masks, creams, or lotions.

[0046] Thirdly, the present invention provides an anti-wrinkle and anti-aging serum, the anti-wrinkle and anti-aging serum comprising the anti-wrinkle and anti-aging composition described in the first aspect, a moisturizer, an emulsifier, a skin moisturizer, a thickener, and a pH neutralizer.

[0047] The anti-wrinkle and anti-aging essence provided by this invention has a reasonable combination of ingredients. Different ingredients intervene in different causes of wrinkles and various stages of skin aging, which can achieve precise anti-aging effects from multiple dimensions. It can achieve the combined effect of inhibiting expression lines, fading existing wrinkles and long-term anti-aging.

[0048] Preferably, the moisturizer comprises any one or a combination of at least two of butylene glycol, glycerin, 1,2-pentanediol, 1,2-hexanediol, propylene glycol, methyl propylene glycol, trehalose, inositol, or betaine.

[0049] Preferably, the emulsifier comprises any one or a combination of at least two of the following: glyceryl stearate, PEG-100 stearate, oil palm oil, oil palm kernel oil, cetearyl alcohol, cocoyl glucoside, polyglycerol-6 distearate, jojoba esters, polyglycerol-3 beeswax ester, or cetyl alcohol.

[0050] Preferably, the emollient comprises any one or a combination of at least two of the following: isononyl isononanoate, shea butter, caprylic / capric triglycerides, octyl dodecyl myristate, squalane, jojoba seed oil, argan kernel oil, or meadowfoam seed oil.

[0051] Preferably, the thickener comprises any one or a combination of at least two of the following: carbomer, xanthan gum, cellulose, hydroxyethyl acrylate / sodium acryloyldimethyl taurate copolymer, or acrylate / C10-3 alkanol acrylate crosspolymer.

[0052] Preferably, the pH neutralizing agent comprises aminopropanol and / or triethanolamine.

[0053] Preferably, the anti-wrinkle and anti-aging essence comprises, by weight, 5-40 parts of the anti-wrinkle and anti-aging composition described in the first aspect, 10-100 parts of moisturizer, 10-50 parts of emulsifier, 10-100 parts of skin moisturizer, 0.1-10 parts of thickener and 0.01-3 parts of pH neutralizer.

[0054] The composition includes 5-40 parts of an anti-wrinkle and anti-aging composition, for example, 5 parts, 10 parts, 15 parts, 20 parts, 25 parts, 30 parts, 35 parts, 40 parts, etc.; 10-100 parts of a moisturizer, for example, 10 parts, 20 parts, 30 parts, 40 parts, 50 parts, 60 parts, 70 parts, 80 parts, 90 parts, 100 parts, etc.; 10-50 parts of an emulsifier, for example, 10 parts, 20 parts, 30 parts, 40 parts, 50 parts, etc.; and a skin moisturizer. 10 to 100 parts, for example, 10 parts, 20 parts, 30 parts, 40 parts, 50 parts, 60 parts, 70 parts, 80 parts, 90 parts, 100 parts, etc.; Thickener 0.1 to 10 parts, for example, 0.1 parts, 1 part, 2 parts, 4 parts, 6 parts, 7 parts, 10 parts, etc.; pH neutralizer 0.01 to 3 parts, for example, 0.01 parts, 0.1 parts, 0.5 parts, 1 part, 1.5 parts, 2 parts, 2.5 parts, 3 parts, etc.

[0055] Fourthly, the present invention provides a method for preparing an anti-wrinkle and anti-aging serum as described in the third aspect, the method comprising:

[0056] (1) Mix the thickener and humectant in water to obtain the water pot material; mix hydroxydecyl ubiquinone, ceramide NP, emollient and emulsifier to obtain the oil pot material.

[0057] (2) Mix and homogenize the water pot material and the oil pot material to obtain a primary mixture.

[0058] (3) Mix the primary mixture obtained in step (2) with a pH neutralizer to obtain a secondary mixture.

[0059] (4) The secondary mixture obtained in step (3) is mixed with hydrolyzed rice protein, Bifida ferment lysate, dipeptide diaminobutyryl benzyl amide diacetate, adenosine, palmitoyl tripeptide-5, carnosine and acetyl hexapeptide-8 to obtain the anti-wrinkle and anti-aging essence.

[0060] Preferably, the mixing temperature before obtaining the water pot material in step (1) is 80~90℃ (for example, it can be 80℃, 82℃, 84℃, 86℃, 88℃, 90℃, etc.), and the mixing time is 5~10min (for example, it can be 5min, 6min, 7min, 8min, 9min, 10min).

[0061] Preferably, the mixing temperature before obtaining the oil pot material in step (1) is 65~75℃ (for example, it can be 65℃, 68℃, 71℃, 73℃, 75℃, etc.), and the mixing time is 5~10min (for example, it can be 5min, 6min, 7min, 8min, 9min, 10min).

[0062] Preferably, the mixing temperature in step (2) is 55~65℃ (e.g., 55℃, 58℃, 61℃, 63℃, 65℃, etc.), and the mixing time is 5~10min (e.g., 5min, 6min, 7min, 8min, 9min, 10min).

[0063] Preferably, the mixing temperature in step (3) is 45~55℃ (e.g., 45℃, 48℃, 51℃, 53℃, 55℃, etc.), and the mixing time is 5~10min (e.g., 5min, 6min, 7min, 8min, 9min, 10min).

[0064] Preferably, the mixing temperature in step (4) is 25~40℃ (e.g., 25℃, 30℃, 35℃, 40℃, etc.), and the mixing time is 10~20min (e.g., 10min, 12min, 14min, 16min, 18min, 20min).

[0065] Preferably, the mixing is carried out by stirring.

[0066] The numerical range described in this invention includes not only the point values ​​listed above, but also any point values ​​within the numerical ranges not listed above. Due to space limitations and for the sake of brevity, this invention will not exhaustively list all the specific point values ​​included in the range.

[0067] Compared with the prior art, the present invention has the following beneficial effects:

[0068] This invention creatively combines hydrolyzed rice protein, bifida ferment lysate, dipeptide diaminobutyryl benzyl amide diacetate, adenosine, hydroxydecyl ubiquinone, ceramide NP, palmitoyl tripeptide-5, carnosine, and acetyl hexapeptide-8. The synergistic effect of these components effectively reduces wrinkles caused by muscle contraction, promotes the synthesis of collagen and elastin, and achieves a combination of inhibiting expression lines, fading existing wrinkles, and long-term anti-aging effects, thus achieving precise anti-wrinkle and anti-aging from multiple dimensions.

[0069] Furthermore, this invention develops a method for preparing hydrolyzed rice protein, which decomposes the complex structure of rice protein to obtain hydrolyzed rice protein with superior anti-wrinkle and anti-aging effects. Detailed Implementation

[0070] To further illustrate the technical means and effects of the present invention, the following describes the technical solution of the present invention in conjunction with preferred embodiments of the present invention. However, the present invention is not limited to the scope of the embodiments.

[0071] The sources of each component / raw material in the following embodiments are shown in Table 1.

[0072] Table 1

[0073]

[0074] Preparation Example 1

[0075] This preparation example provides a hydrolyzed rice protein, the preparation method of which is as follows:

[0076] (1) 1000g of rice was crushed through an 80-mesh sieve and dried to obtain rice powder. The rice powder was mixed with 3L of 0.1mol / L NaOH aqueous solution at 25℃ and stirred for 2.5h. Then, it was subjected to ultra-high pressure treatment at 350MPa for 10min to obtain pretreated solution.

[0077] (2) The pretreatment solution obtained in step (1) is mixed with cellulase and saccharifying enzyme (the amount of cellulase added in the pretreatment solution is 50 U / g and the amount of saccharifying enzyme added in the pretreatment solution is 40 U / g), the pH value is adjusted to 8.5, and the enzyme is hydrolyzed at 85℃ for 3 h. The solid and liquid are separated to obtain a solid product. Then the solid product is dispersed in 50 mL of water to obtain mixture I.

[0078] (3) Mix the mixture I obtained in step (2) with trypsin for enzymatic hydrolysis (the amount of trypsin added to the mixture I is 80 U / g), adjust the pH value to 8, and enzymatically hydrolyze at 65℃ for 2 h. Separate the solid and liquid to obtain the solid product, and then disperse the solid product in 15 mL of water to obtain mixture II.

[0079] (4) Mix the mixture II obtained in step (3) with papain and neutral protease (the amount of papain added to mixture II is 50 U / g and the amount of neutral protease added to mixture II is 30 U / g), adjust the pH value to 7, and enzymatically hydrolyze at 50℃ for 2 h to obtain mixture III.

[0080] (5) Use an ultrafiltration membrane with a molecular weight cutoff of 3 kDa to ultrafilter the mixture III obtained in step (4). The centrifugal force of ultrafiltration is 7000 x g. Concentrate the filtrate to 20% of the original volume. Freeze-dry the concentrate to obtain hydrolyzed rice protein.

[0081] Preparation Example 2

[0082] This preparation example provides a hydrolyzed rice protein, the preparation method of which is as follows:

[0083] (1) 1000g of rice was crushed through a 60-mesh sieve and dried to obtain rice powder. The rice powder was mixed with 3L of 0.5mol / L NaHCO3 aqueous solution at 35℃ and stirred for 2h. Then, it was subjected to ultra-high pressure treatment at 450MPa for 5min to obtain pretreated solution.

[0084] (2) The pretreatment solution obtained in step (1) is mixed with cellulase and saccharifying enzyme (the amount of cellulase added in the pretreatment solution is 45 U / g and the amount of saccharifying enzyme added in the pretreatment solution is 50 U / g), the pH value is adjusted to 9, and the enzyme is enzymatically hydrolyzed at 80℃ for 3 h. Solid-liquid separation is performed to obtain solid product, and then the solid product is dispersed in 50 mL of water to obtain mixture I.

[0085] (3) Mix the mixture I obtained in step (2) with trypsin for enzymatic hydrolysis (the amount of trypsin added to mixture I is 70 U / g), adjust the pH value to 7.5, and enzymatically hydrolyze at 70℃ for 3 h. Separate the solid and liquid to obtain the solid product, and then disperse the solid product in 15 mL of water to obtain mixture II.

[0086] (4) Mix the mixture II obtained in step (3) with papain and neutral protease (the amount of papain added to mixture II is 60 U / g and the amount of neutral protease added to mixture II is 10 U / g), adjust the pH value to 6, and enzymatically hydrolyze at 40℃ for 2 h to obtain mixture III.

[0087] (5) Use an ultrafiltration membrane with a molecular weight cutoff of 4 kDa to ultrafilter the mixture III obtained in step (4). The centrifugal force of ultrafiltration is 7000 x g. Concentrate the filtrate to 20% of the original volume. Freeze-dry the concentrate to obtain hydrolyzed rice protein.

[0088] Preparation Example 3

[0089] This preparation example provides a hydrolyzed rice protein, the preparation method of which is as follows:

[0090] (1) 1000g of rice was crushed through a 70-mesh sieve and dried to obtain rice powder. The rice powder was mixed with 3L of 0.3mol / L NaOH aqueous solution at 40℃ and stirred for 3h. Then, it was subjected to ultra-high pressure treatment at 300MPa for 8min to obtain pretreated solution.

[0091] (2) The pretreatment solution obtained in step (1) is mixed with cellulase and saccharifying enzyme (the amount of cellulase added in the pretreatment solution is 60 U / g and the amount of saccharifying enzyme added in the pretreatment solution is 30 U / g), the pH value is adjusted to 7.5, and the enzyme is hydrolyzed at 70℃ for 4 h. The solid and liquid are separated to obtain a solid product. Then the solid product is dispersed in 50 mL of water to obtain mixture I.

[0092] (3) Mix the mixture I obtained in step (2) with trypsin for enzymatic hydrolysis (the amount of trypsin added to mixture I is 90 U / g), adjust the pH value to 7, and enzymatically hydrolyze at 55℃ for 1.5 h. Separate the solid and liquid to obtain the solid product, and then disperse the solid product in 15 mL of water to obtain mixture II.

[0093] (4) Mix the mixture II obtained in step (3) with papain and neutral protease (the amount of papain added to mixture II is 40 U / g and the amount of neutral protease added to mixture II is 20 U / g), adjust the pH value to 5.5, and enzymatically hydrolyze at 35℃ for 3 h to obtain mixture III.

[0094] (5) Use an ultrafiltration membrane with a molecular weight cutoff of 2kDa to ultrafilter the mixture III obtained in step (4). The centrifugal force of ultrafiltration is 7000xg. Concentrate the filtrate to 20% of the original volume and freeze-dry the concentrate to obtain hydrolyzed rice protein.

[0095] Preparation Example 4

[0096] This preparation example provides a hydrolyzed rice protein. The only difference between this preparation method and that of Preparation Example 1 is that step (3) is removed, and the mixture I obtained in step (2) is directly processed in step (4). The rest of the preparation methods are consistent with those of Preparation Example 1.

[0097] Preparation Example 5

[0098] This preparation example provides a hydrolyzed rice protein. The only difference between this preparation method and that of Preparation Example 1 is that step (2) is removed, and the pretreatment solution obtained in step (1) is directly processed in step (3). The rest of the preparation methods are consistent with those of Preparation Example 1.

[0099] Preparation Example 6

[0100] This preparation example provides a hydrolyzed rice protein. The only difference between this preparation example and preparation example 1 is that no saccharifying enzyme is added in step (2). All other preparation methods are the same as those in preparation example 1.

[0101] Preparation Example 7

[0102] This preparation example provides a hydrolyzed rice protein. The only difference between this preparation example and preparation example 1 is that cellulase is not added in step (2). All other preparation methods are the same as those in preparation example 1.

[0103] Example 1

[0104] This embodiment provides an anti-wrinkle and anti-aging composition, which, by weight, comprises 2 parts of hydrolyzed rice protein prepared in Preparation Example 1, 0.8 parts of Bifida ferment lysate, 5 parts of dipeptide diaminobutyryl benzylamide diacetate, 0.2 parts of adenosine, 0.2 parts of hydroxydecyl ubiquinone, 0.3 parts of ceramide NP, 3 parts of palmitoyl tripeptide-5, 0.2 parts of carnosine, and 5 parts of acetyl hexapeptide-8.

[0105] Example 2

[0106] This embodiment provides an anti-wrinkle and anti-aging composition, which, by weight, comprises 1.5 parts of hydrolyzed rice protein prepared in Preparation Example 2, 0.5 parts of Bifida ferment lysate, 4 parts of dipeptide diaminobutyryl benzylamide diacetate, 0.1 parts of adenosine, 0.05 parts of hydroxydecyl ubiquinone, 0.1 parts of ceramide NP, 5 parts of palmitoyl tripeptide-5, 0.3 parts of carnosine, and 2 parts of acetyl hexapeptide-8.

[0107] Example 3

[0108] This embodiment provides an anti-wrinkle and anti-aging composition, which, by weight, comprises 1 part of hydrolyzed rice protein prepared in Preparation Example 3, 1 part of Bifida ferment lysate, 6 parts of dipeptide diaminobutyryl benzylamide diacetate, 0.3 parts of adenosine, 0.3 parts of hydroxydecyl ubiquinone, 0.2 parts of ceramide NP, 0.05 parts of palmitoyl tripeptide-5, 0.5 parts of carnosine, and 8 parts of acetyl hexapeptide-8.

[0109] Examples 4-7

[0110] Examples 4-7 each provide an anti-wrinkle and anti-aging composition, which differs from Example 1 only in that the hydrolyzed rice protein prepared in Preparation Example 1 is replaced with the hydrolyzed rice protein prepared in Preparation Examples 4-7 respectively, while the rest of the formulation is the same as that in Example 1.

[0111] Example 8

[0112] This embodiment provides an anti-wrinkle and anti-aging composition, which differs from Example 1 only in that 2 parts of hydrolyzed rice protein, 0.8 parts of Bifida ferment lysate, and 5 parts of dipeptide diaminobutyryl benzyl amide diacetate prepared in Example 1 are replaced with 5 parts of hydrolyzed rice protein, 0.2 parts of Bifida ferment lysate, and 8 parts of dipeptide diaminobutyryl benzyl amide diacetate prepared in Example 1. The rest of the formulation remains the same as in Example 1.

[0113] Example 9

[0114] This embodiment provides an anti-wrinkle and anti-aging composition, which differs from Example 1 only in that 0.2 parts of adenosine, 0.2 parts of hydroxydecyl ubiquinone, and 0.3 parts of ceramide NP are replaced with 0.4 parts of adenosine, 0.005 parts of hydroxydecyl ubiquinone, and 0.5 parts of ceramide NP. The rest of the formulation remains the same as in Example 1.

[0115] Comparative Example 1

[0116] This comparative example provides an anti-wrinkle and anti-aging composition, which differs from Example 1 only in that hydrolyzed rice protein is not added. The insufficient portion is made up by Bifida ferment lysate and dipeptide diaminobutyryl benzyl amide diacetate in a mass ratio of 0.8:5. All other formulations are consistent with Example 1.

[0117] Comparative Example 2

[0118] This comparative example provides an anti-wrinkle and anti-aging composition, which differs from Example 1 only in that it does not contain bifida ferment lysate. The insufficient portion is made up by hydrolyzed rice protein and dipeptide diaminobutyryl benzylamide diacetate in a mass ratio of 2:5. All other formulations are consistent with Example 1.

[0119] Comparative Example 3

[0120] This comparative example provides an anti-wrinkle and anti-aging composition, which differs from Example 1 only in that it does not contain dipeptide diaminobutyryl benzylamide diacetate. The insufficient portion is made up by hydrolyzed rice protein and Bifida ferment lysate in a mass ratio of 2:0.8. All other formulations are consistent with Example 1.

[0121] Comparative Example 4

[0122] This comparative example provides an anti-wrinkle and anti-aging composition, which differs from Example 1 only in that adenosine is not added, and the insufficient portion is made up by hydroxydecyl ubiquinone and ceramide NP in a mass ratio of 0.2:0.3. All other formulations are consistent with Example 1.

[0123] Comparative Example 5

[0124] This comparative example provides an anti-wrinkle and anti-aging composition, which differs from Example 1 only in that hydroxydecyl ubiquinone is not added, and the insufficient portion is made up by adenosine and ceramide NP in a mass ratio of 0.2:0.3.

[0125] Comparative Example 6

[0126] This comparative example provides an anti-wrinkle and anti-aging composition, which differs from Example 1 only in that ceramide NP is not added, and the insufficient part is made up by adenosine and hydroxydecyl ubiquinone in a mass ratio of 0.2:0.2. The rest of the formulation is the same as that of Example 1.

[0127] Application Example 1

[0128] This application example provides an anti-wrinkle and anti-aging serum, the formula of which is shown in Table 2.

[0129] Table 2

[0130]

[0131] The preparation method of the anti-wrinkle and anti-aging serum is as follows:

[0132] (1) Mix the thickener and humectant in water and stir at 85°C for 7 minutes to obtain the water pot material; mix hydroxydecyl ubiquinone, ceramide NP, emollient and emulsifier and stir at 70°C for 7 minutes to obtain the oil pot material.

[0133] (2) Mix the water pot material and the oil pot material, stir at 60°C for 8 minutes to homogenize, and obtain the primary mixture.

[0134] (3) Mix the primary mixture obtained in step (2) with the pH neutralizer and stir at 50°C for 8 minutes to obtain the secondary mixture.

[0135] (4) The secondary mixture obtained in step (3) is mixed with hydrolyzed rice protein, Bifida ferment lysate, dipeptide diaminobutyryl benzyl amide diacetate, adenosine, palmitoyl tripeptide-5, carnosine, and acetyl hexapeptide-8, and stirred at 35°C for 15 min to obtain the anti-wrinkle and anti-aging essence.

[0136] Application Example 2

[0137] This application example provides an anti-wrinkle and anti-aging serum, the formula of which is shown in Table 3.

[0138] Table 3

[0139]

[0140] The preparation method of the anti-wrinkle and anti-aging serum is as follows:

[0141] (1) Mix the thickener and humectant in water and stir at 80°C for 10 min to obtain the water pot material; mix hydroxydecyl ubiquinone, ceramide NP, emollient and emulsifier and stir at 65°C for 10 min to obtain the oil pot material.

[0142] (2) Mix the water pot material and the oil pot material, stir at 55℃ for 10 minutes to homogenize and obtain a primary mixture.

[0143] (3) Mix the primary mixture obtained in step (2) with the pH neutralizer and stir at 45°C for 10 min to obtain the secondary mixture.

[0144] (4) The secondary mixture obtained in step (3) is mixed with hydrolyzed rice protein, Bifida ferment lysate, dipeptide diaminobutyryl benzyl amide diacetate, adenosine, palmitoyl tripeptide-5, carnosine, and acetyl hexapeptide-8, and stirred at 25°C for 20 min to obtain the anti-wrinkle and anti-aging essence.

[0145] Application Example 3

[0146] This application example provides an anti-wrinkle and anti-aging serum, the formula of which is shown in Table 4.

[0147] Table 4

[0148]

[0149] The preparation method of the anti-wrinkle and anti-aging serum is as follows:

[0150] (1) Mix the thickener and humectant in water and stir at 80°C for 10 min to obtain the water pot material; mix hydroxydecyl ubiquinone, ceramide NP, emollient and emulsifier and stir at 65°C for 10 min to obtain the oil pot material.

[0151] (2) Mix the water pot material and the oil pot material, stir at 55℃ for 10 minutes to homogenize and obtain a primary mixture.

[0152] (3) Mix the primary mixture obtained in step (2) with the pH neutralizer and stir at 45°C for 10 min to obtain the secondary mixture.

[0153] (4) The secondary mixture obtained in step (3) is mixed with hydrolyzed rice protein, Bifida ferment lysate, dipeptide diaminobutyryl benzyl amide diacetate, adenosine, palmitoyl tripeptide-5, carnosine, and acetyl hexapeptide-8, and stirred at 25°C for 20 min to obtain the anti-wrinkle and anti-aging essence.

[0154] Application Example 4-9

[0155] Application Examples 4-9 each provide an anti-wrinkle and anti-aging serum. The only difference between their formulations and Application Example 1 is that each component in the anti-wrinkle and anti-aging composition of Example 1 is replaced with each component in the anti-wrinkle and anti-aging compositions of Examples 4-9, and the preparation method is the same as that of Application Example 1.

[0156] Comparative Application Examples 1-6

[0157] Comparative Application Examples 1-6 each provide an anti-wrinkle and anti-aging serum. The only difference between them and Application Example 1 is that each component in the anti-wrinkle and anti-aging composition of Example 1 is replaced with each component in the anti-wrinkle and anti-aging compositions of Comparative Examples 1-6 in turn. The preparation method is the same as that of Application Example 1.

[0158] Test Example 1

[0159] Evaluation of DPPH free radical scavenging ability:

[0160] The ability to scavenge DPPH (1,1-diphenyl-2-trinitrophenylhydrazine) free radicals reflects, to some extent, a substance's antioxidant capacity. A higher free radical scavenging rate indicates stronger antioxidant and anti-aging capabilities. Therefore, the anti-aging effect of anti-aging compositions can be assessed by studying their ability to scavenge DPPH free radicals.

[0161] The anti-wrinkle and anti-aging compositions prepared in Examples 1-9 and Comparative Examples 1-6 were diluted with DMSO to a mass fraction of 2% for the test samples, and the following tests were conducted:

[0162] Add 2.0 mL of DPPH test solution (45.8 mg / L) and the sample to be tested to a test tube. Make up the total volume to 3 mL with anhydrous ethanol, shake well, and react in the dark for 30 min. Measure the absorbance at 517 nm using a 1 cm cuvette, and record it as A. 10 Add 2 mL of anhydrous ethanol and the corresponding volume of the sample to be tested to the test tube, and make up the total volume to 3 mL with anhydrous ethanol. Record the measured absorbance as A. 11 Add 2 mL of DPPH test solution and 1 mL of anhydrous ethanol to a test tube, and record the absorbance as A. 12 Calculate the DPPH free radical scavenging rate (Y') of the test solution.

[0163] The formula for calculating the DPPH free radical scavenging rate is: ;

[0164] In the formula A 10 A represents the absorbance value of the system after DPPH removal from the sample. 11 A represents the absorbance value of the system after DPPH removal from the sample blank control. 12 The absorbance value is the absorbance of the reaction system before the sample to be tested is added.

[0165] The test results are shown in Table 5.

[0166] Test Example 2

[0167] Evaluation of hydroxyl radical scavenging ability:

[0168] Add 3 mL of 2 mmol / L FeSO4 and 3 mL of 1 mmol / L H2O2 sequentially to a 25 mL colorimetric tube, shake well, then add 3 mL of 6 mmol / L salicylic acid, shake well, heat in a 37℃ water bath for 15 min, and measure the absorbance. Add a certain concentration of the test solution, shake well, and continue heating in a water bath for 15 min, then measure the absorbance. The following formula represents the scavenging rate of the test solution for hydroxyl radicals (·OH):

[0169] Hydroxyl radical scavenging rate (%) = [A1-A2-(A1-A3)] / A1×100%; where A1 is the absorbance value of the reaction system before the addition of the drug; A2 is the absorbance value of the system after the sample removes ·OH; and A3 is the absorbance value of the system after the blank control removes ·OH. The test results are shown in Table 5.

[0170] Table 5

[0171]

[0172] As shown in Table 5, the anti-wrinkle and anti-aging composition of this invention has excellent antioxidant capacity, with a DPPH free radical scavenging rate of over 40% and a hydroxyl free radical scavenging rate of over 40%. Examples 1 and Comparative Examples 1-3 show that when any one of the components—hydrolyzed rice protein, Bifida ferment lysate, or dipeptide diaminobutyryl benzyl amide diacetate—is removed, the antioxidant capacity decreases, indicating a good synergistic effect among the three components in terms of antioxidant capacity. Examples 1 and Comparative Examples 4-6 show that when any one of the components—adenosine, hydroxydecyl ubiquinone, or ceramide NP—is removed, the antioxidant capacity also decreases to varying degrees, indicating a good synergistic effect among the three components in terms of antioxidant capacity. As shown in Examples 1 and 4-5, when an enzymatic hydrolysis step is omitted during the preparation of hydrolyzed rice protein, the antioxidant capacity is not as good as in Examples 1-3. As shown in Examples 1 and 6-7, when either cellulase or saccharifying enzyme is missing in the first enzymatic hydrolysis step during the preparation of hydrolyzed rice protein, the DPPH free radical scavenging capacity and hydroxyl free radical scavenging capacity of the composition will decrease. As shown in Examples 1 and 8-9, when the mass ratio of hydrolyzed rice protein, Bifida ferment lysate, and dipeptide diaminobutyryl benzyl amide diacetate is not greater than (1-3):(0.5-1):(4-6), or when the mass ratio of adenosine, hydroxydecyl ubiquinone, and ceramide NP is not greater than (0.1-0.3):(0.01-0.3):(0.1-0.3), the antioxidant capacity of the composition will decrease to a certain extent.

[0173] Test Example 3

[0174] Wrinkle-reducing effect test:

[0175] 150 subjects aged 20-45 years were randomly selected, including 75 males and 75 females. The selected subjects presented with fine lines, noticeable wrinkles, and sagging skin. The subjects were divided into 15 groups. After cleansing their skin, each group applied an appropriate amount of the serum products from Examples 1-9 and Control Examples 1-6 evenly to the entire face and eye area, gently massaging until fully absorbed. This was done twice daily, morning and evening, for two consecutive months. Skin images were captured using the VISIA-CR facial image analyzer to measure the proportion of wrinkles. Evaluation criteria: The difference in wrinkle proportion refers to the difference between the proportion of wrinkles after product use and the proportion before product use. A negative value indicates a reduction in wrinkles after product use. The results are shown in Table 6.

[0176] Table 6

[0177]

[0178] Test Example 4

[0179] Skin firming effect test:

[0180] The same group of subjects from Test Example 3 were selected, and this group also underwent the following skin tightening effect test. Each group used the serum products from Application Examples 1-9 and Control Examples 1-6, respectively. Subjects first cleansed their faces for the same amount of time. After the skin dried, a 5cm × 5cm area of ​​skin on the cheek was selected as the test area. 1mL of the serum prepared in Application Examples 1-9 and Control Examples 1-6 was evenly applied to the test area on the face. The skin elasticity R² value was measured using a skin elasticity meter (Cutometer MPA580, CK GmbH, Germany) before product use and after 4 weeks of continuous use. The skin elasticity R² value for each test area was the average of 5 measurements. Then, the average value of all volunteers' measurements was taken. The closer the skin elasticity R² value is to 1, the better the skin elasticity. The results are shown in Table 7.

[0181] Table 7

[0182]

[0183] As can be seen from the test data in Tables 6 and 7, the anti-wrinkle and anti-aging essence involved in this invention has excellent wrinkle removal ability and skin firming effect, and has both anti-wrinkle and anti-aging properties.

[0184] As shown in Application Example 1 and Comparative Application Examples 1-3, when any one of the components—hydrolyzed rice protein, Bifida ferment lysate, or dipeptide diaminobutyryl benzyl amide diacetate—was removed, both the wrinkle-removing ability and skin-tightening effect decreased, indicating that the three components have a good synergistic effect in anti-wrinkle and anti-aging. As shown in Example 1 and Comparative Examples 4-6, when any one of the components—adenosine, hydroxydecyl ubiquinone, or ceramide NP—was removed, both the wrinkle-removing ability and skin-tightening effect also decreased, indicating that the three components have a good synergistic effect in anti-wrinkle and anti-aging. As shown in Examples 1 and 4-5, when an enzymatic hydrolysis step is omitted in the preparation of hydrolyzed rice protein, the wrinkle removal ability and skin tightening effect are not as good as in Examples 1-3. As shown in Examples 1 and 6-7, when either cellulase or glucoamylase is missing in the first enzymatic hydrolysis step in the preparation of hydrolyzed rice protein, the anti-wrinkle and anti-aging effects of the essence will decrease. As shown in Examples 1 and 8-9, when the mass ratio of hydrolyzed rice protein, Bifida ferment lysate, and dipeptide diaminobutyryl benzyl amide diacetate is not greater than (1-3):(0.5-1):(4-6), or when the mass ratio of adenosine, hydroxydecyl ubiquinone, and ceramide NP is not greater than (0.1-0.3):(0.01-0.3):(0.1-0.3), the wrinkle removal and anti-aging abilities of the essence will decrease to a certain extent.

[0185] The applicant declares that the technical solution of this invention is illustrated by the above embodiments, but this invention is not limited to the above embodiments, that is, it does not mean that this invention must rely on the above embodiments to be implemented. Those skilled in the art should understand that any improvements to this invention, equivalent substitutions of raw materials for the products of this invention, addition of auxiliary components, selection of specific methods, etc., all fall within the protection scope and disclosure scope of this invention.

[0186] The preferred embodiments of the present invention have been described in detail above. However, the present invention is not limited to the specific details in the above embodiments. Within the scope of the technical concept of the present invention, various simple modifications can be made to the technical solution of the present invention, and these simple modifications all fall within the protection scope of the present invention.

[0187] It should also be noted that the various specific technical features described in the above specific embodiments can be combined in any suitable manner without contradiction. In order to avoid unnecessary repetition, the present invention will not describe the various possible combinations separately.

Claims

1. An anti-wrinkle anti-aging composition characterized in that, The anti-wrinkle and anti-aging composition comprises hydrolyzed rice protein, Schizochytrium ferment lysate, dipeptide diaminobutyryl benzyl amide diacetate, adenosine, hydroxydecyl ubiquinone, ceramide NP, palmitoyl tripeptide-5, carnosine and acetyl hexapeptide-8.

2. The anti-wrinkle anti-aging composition according to claim 1, characterized in that, The anti-wrinkle and anti-aging composition comprises, in parts by weight, 1-3 parts of hydrolyzed rice protein, 0.5-2 parts of Schizochytrium ferment lysate, 1.5-6 parts of dipeptide diaminobutyryl benzyl amide diacetate, 0.03-0.3 parts of adenosine, 0.01-0.3 parts of hydroxydecyl ubiquinone, 0.1-1 part of ceramide NP, 0.5-5 parts of palmitoyl tripeptide-5, 0.05-0.3 parts of carnosine and 1-10 parts of acetyl hexapeptide-8. Preferably, the mass ratio of the hydrolyzed rice protein, the Schizochytrium ferment lysate and the dipeptide diaminobutyryl benzyl amide diacetate is (1-3):(0.5-1):(4-6). Preferably, the mass ratio of the adenosine, the hydroxydecyl ubiquinone and the ceramide NP is (0.1-0.3):(0.01-0.3):(0.1-0.3).

3. The anti-wrinkle anti-aging composition according to claim 1 or 2, characterized in that, The hydrolyzed rice protein is prepared by a method comprising the following steps: (1) mixing rice powder with alkali liquor and then performing ultrahigh pressure treatment to obtain pretreated liquid; (2) mixing the pretreated liquid with cellulase and saccharifying enzyme for enzymolysis, performing solid-liquid separation, obtaining solid product, then dispersing the solid product in water to obtain mixed liquid I; (3) mixing the mixed liquid I obtained in step (2) with trypsin for enzymolysis, performing solid-liquid separation, obtaining solid product, then dispersing the solid product in water to obtain mixed liquid II; (4) mixing the mixed liquid II obtained in step (3) with papain and / or neutral protease for enzymolysis to obtain mixed liquid III; (5) performing ultrafiltration on the mixed liquid III obtained in step (4), concentrating and drying the filtrate to obtain the hydrolyzed rice protein.

4. The anti-wrinkle anti-aging composition according to claim 3, characterized in that, The alkali liquor in step (1) comprises NaOH aqueous solution or NaHCO3 aqueous solution; Preferably, the temperature for mixing in step (1) is 20-40℃, and the mixing time is 2-4h; Preferably, the mixing is performed by stirring; Preferably, the pressure for ultrahigh pressure treatment in step (1) is 300-500MPa, and the ultrahigh pressure treatment time is 5-10min; Preferably, the temperature for enzymolysis in step (2) is 70-90℃, and the enzymolysis time is 1-4h; Preferably, the enzymolysis in step (2) is performed at pH value of 7-10; Preferably, the cellulase is added in the pretreated liquid in an amount of 40-70U / g, and the saccharifying enzyme is added in the pretreated liquid in an amount of 30-60U / g; Preferably, the temperature for enzymolysis in step (3) is 55-70℃, and the enzymolysis time is 1-4h; Preferably, the enzymolysis in step (3) is performed at pH value of 7-10; Preferably, the trypsin is added in the mixed liquid I in an amount of 60-90U / g.

5. The anti-wrinkle anti-aging composition according to claim 3 or 4, characterized in that, Step (4) is to mix the mixed solution II obtained in step (3) with papain and neutral protease to perform enzymolysis, and obtain a mixed solution III; Preferably, the added amount of the papain in the mixed solution II is 40-60 U / g; Preferably, the added amount of the neutral protease in the mixed solution II is 10-30 U / g; Preferably, the temperature of the enzymolysis in step (4) is 30-60℃, and the enzymolysis time is 1-4 h; Preferably, the enzymolysis in step (4) is performed at a pH value of 5-7; Preferably, the membrane molecular weight cut-off of the ultrafiltration in step (5) is 2-4 kDa.

6. Use of the anti-wrinkle and anti-aging composition according to any one of claims 1-5 in cosmetics.

7. An anti-wrinkle anti-aging serum, characterized in that, The anti-wrinkle and anti-aging serum comprises the anti-wrinkle and anti-aging composition according to any one of claims 1-5, a humectant, an emulsifier, an emollient, a thickening agent, and a pH neutralizer.

8. The anti-wrinkle and anti-aging serum according to claim 7, characterized in that, The humectant comprises any one or a combination of at least two of butylene glycol, glycerin, 1,2-pentanediol, 1,2-hexanediol, propylene glycol, methylpropanediol, trehalose, inositol, or betaine; Preferably, the emulsifier comprises any one or a combination of at least two of glyceryl stearate, PEG-100 stearate, olibanum oil, olibanum kernel oil, cetyl stearyl alcohol, cocoglycoside, polyglyceryl-6 distearate, jojoba esters, polyglyceryl-3 beeswaxate, or cetyl alcohol; Preferably, the emollient comprises any one or a combination of at least two of isononyl isononanoate, shea butter, caprylic / capric glycerides, octyldodecyl myristate, squalane, jojoba seed oil, argania spinosa kernel oil, or simmondsia chinensis seed oil; Preferably, the thickening agent comprises any one or a combination of at least two of carbomer, xanthan gum, cellulose, hydroxyethyl acrylate / sodium acryloyldimethyl taurate copolymer, or acrylate / C10-30 alkyl acrylate crosspolymer; Preferably, the pH neutralizer comprises aminomethyl propanol and / or triethanolamine; Preferably, the anti-wrinkle and anti-aging serum comprises the anti-wrinkle and anti-aging composition according to any one of claims 1-5 in an amount of 5-40 parts by weight, the humectant in an amount of 10-100 parts by weight, the emulsifier in an amount of 10-50 parts by weight, the emollient in an amount of 10-100 parts by weight, the thickening agent in an amount of 0.1-10 parts by weight, and the pH neutralizer in an amount of 0.01-3 parts by weight.

9. A method of preparing the anti-wrinkle and anti-aging serum according to claim 7 or 8, characterized in that, The preparation method comprises: (1) mixing a thickening agent and a humectant in water to obtain a water pot material, and mixing hydroxydecyl ubiquinone, ceramide NP, an emollient, and an emulsifier to obtain an oil pot material; (2) mixing and homogenizing the water pot material and the oil pot material to obtain a primary mixed solution; (3) mixing the primary mixed solution obtained in step (2) with a pH neutralizer to obtain a secondary mixed solution; (4) mixing the secondary mixed solution obtained in step (3) with hydrolyzed rice protein, saccharomyces boulardii ferment lysate, dipeptide diaminobutyroyl hydrazide dipalmitoyl tripeptide-9, adenosine, palmitoyl tetrapeptide-7, and acetyl hexapeptide-8 to obtain the anti-wrinkle and anti-aging serum.

10. The method for preparing the anti-wrinkle and anti-aging serum according to claim 9, characterized in that, The temperature of the mixing before obtaining the water pot material body in step (1) is 80-90℃, and the mixing time is 5-10 min; Preferably, the temperature of the mixing before obtaining the oil pot material body in step (1) is 65-75℃, and the mixing time is 5-10 min; Preferably, the temperature of the mixing in step (2) is 55-65℃, and the mixing time is 5-10 min; Preferably, the temperature of the mixing in step (3) is 45-55℃, and the mixing time is 5-10 min; Preferably, the temperature of the mixing in step (4) is 25-40℃, and the mixing time is 10-20 min; Preferably, the mixing is carried out in the form of stirring.

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