272 results about "Dipeptide" patented technology

The invention belongs to the field of skin care products, and particularly relates to an anti-wrinkle and anti-aging composition as well as a preparation method and application thereof, and the anti-wrinkle and anti-aging composition comprises neurotransmitter inhibition peptide, signal peptide, madecassoside, inositol and glycosylglycerol. The neurotransmitter inhibition type peptide is at least three of acetyl hexapeptide-8, arginine / lysine polypeptide, dipeptide diaminobutyrylbenzylamide diacetate and acetyl octapeptide-3, and the signal type peptide is at least two of palmitoyl pentapeptide-4, decapeptide-4 and acetyl tetrapeptide-9. Compared with the prior art, all the components of the anti-wrinkle and anti-aging composition act on different anti-aging pathways and can be matched with one another from multiple aspects of oxidation resistance, inflammation resistance, moisturizing, cell viability enhancement, wrinkle reduction and the like, a more comprehensive and better anti-aging effect is provided, skin aging is comprehensively resisted, the skin is in a more young state, and the anti-wrinkle and anti-aging composition has the advantages that the anti-wrinkle and anti-aging effects are achieved. And the anti-wrinkle and anti-aging composition is small in irritation and good in stability.

Provided are an antibacterial peptide and the use thereof. The antibacterial peptide has a structure represented by formula (I), wherein Z1 is selected from tetrapeptides, the tetrapeptides being optionally modified by -OH, -CHO, -COOH, -NH2, -SO3H or -C=O; S1 and S2 are each independently selected from amino acids having the structure of (I); Q1 and Q2 are each independently selected from dipeptides or tripeptides; and n is independently selected from any integer between 1 and 6. (Q2)4-(S2-Q1)2-S1-Z1

The invention relates to an anti-wrinkle and anti-aging composition and application thereof. The anti-wrinkle and anti-aging composition is prepared from hydrolyzed rice protein, a bifidus yeast fermentation product lysate, dipeptide diaminobutyryl benzyl amide diacetate, adenosine, hydroxydecyl ubiquinone, ceramide NP, palmitoyl tripeptide-5, carnosine and acetyl hexapeptide-8. According to the present invention, the components achieve the synergistic effect, such that the fibroblast proliferation can be promoted, the cell metabolism can be improved, the generation of collagen, elastin and the like can be promoted, the excellent oxidation resistance can be provided, and the effects of expression line inhibition, existing wrinkle fading and long-term aging resistance can be combined.

The invention discloses a bipeptide modified bionic nano-vesicle as well as a preparation method and application thereof, and belongs to the field of biological medicines. The dipeptide modified bionic nano-vesicle comprises nano-particles formed by PLGA (poly (lactic-co-glycolic acid)), and the nano-particles are loaded with a medicine with a nerve protection or nerve repair effect; the surface of the nanoparticle is coated with a macrophage membrane for expressing RVG peptide and T7 peptide. The bipeptide modified bionic nano-vesicle simultaneously presents T7 peptide and RVG peptide through an engineered macrophage membrane, the T7 peptide is combined with a blood-brain barrier transferrin receptor through high affinity to realize efficient brain entry, astrocytes in the brain are specifically recognized by virtue of the RVG peptide, accurate recognition and delivery of target cells in a focus area are realized, and the bipeptide modified bionic nano-vesicle has a good application prospect. Meanwhile, the natural inflammation tropism and immune escape ability of a macrophage membrane are reserved, and the problems that a traditional drug delivery system is low in targeting precision, and cross-barrier distribution and intracerebral distribution are difficult to cooperate are solved.

The invention relates to the technical field of polypeptide biosynthesis, in particular to a method for biosynthesizing acetyl tetrapeptide-5, a composition containing the acetyl tetrapeptide-5 and application of the composition. According to the method, low-cost natural amino acid is used as a substrate, specific aminopeptidase or a mutant thereof is used as a biocatalyst, amino acid ligase and polyphosphatase are combined, and acetyl tetrapeptide-5 can be efficiently synthesized through a one-step enzymatic reaction. Compared with a traditional chemical synthesis method, the method has the advantages of low raw material cost, no use of an organic solvent, mild reaction conditions, high regioselectivity, environmental friendliness, low production cost and the like. The invention also provides a composition containing acetyl tetrapeptide-5 and dipeptide-15, the inhibition effect of the composition on grease secretion is obviously better than that of a single component, and the composition has a synergistic oil control effect under a specific ratio.

The invention relates to the technical field of medicines, in particular to a preparation method of semeglutide, which comprises the following steps: by optimizing a coupling sequence, firstly connecting a main peptide chain compound 1 with a side chain compound 5 without carboxyl protection, and then performing subsequent coupling with a dipeptide structure compound 6 with high steric hindrance, thereby obtaining the semeglutide. The problem of excessive modification caused by coexistence of a plurality of active sites in a traditional route is effectively avoided, especially for coupling difficulty brought by the structure of a compound 6, the types of a condensation reagent and a side-chain compound 5 are optimized, dipeptide carboxyl of the compound 5 is remarkably activated, amido bonds can be efficiently formed by the compound 5 and a compound 2 under the mild condition, and the compound 6 can be efficiently synthesized. In the path of synthesizing the compound 3 from the compound 1, one-pot operation is realized, and the reaction liquid in each step can be used for the next step without refining, so that the reaction time and the consumption of raw materials are reduced, and the connection efficiency and selectivity of key steps are greatly improved.

The invention provides a method for synthesizing N-methylated polypeptide, which comprises the following steps: dissolving a carboxylic acid compound, N-methyl amino-acid ester hydrochloride or N-methyl amino-acid ester, an alkaline substance and pivaloic anhydride in an organic solvent, reacting at 15-80 DEG C for 3-10 hours, and post-treating the obtained reaction liquid to obtain an N-methyl dipeptide compound, the method comprises the following steps: removing a protecting group in an N-methyl dipeptide compound to obtain a free-state N-methyl dipeptide compound, and condensing the free-state N-methyl dipeptide compound and amino acid or N-methyl amino-acid ester protected by amino to obtain the N-methylated polypeptide, the mixed anhydride intermediate is formed in situ, and the reaction is completed in one step; according to the method, trimethylacetic anhydride is used as a condensing agent, the structure is simple, the reaction is safe, cheap and non-toxic, a large amount of nitrogen-containing byproducts are not generated in the reaction, and purification is convenient; the method is high in stereoselectivity, and racemization is avoided; according to the method, environment-friendly solvents such as ethyl acetate can be used, the reaction condition is mild, and the reaction time is short.

The disclosure provides the development of enantiomer-specific 7V-arylpyrazole dipeptides as novel N0D2 agonists which are effective at promoting immune checkpoint inhibitor therapy requiring N0D2 for activity. Given the significant functions of N0D2 in innate and adaptive immunity, these novel agonists afford new therapeutic compounds for a variety of NOD2-responsive diseases.

The invention belongs to the field of bionic electronic materials and application, and discloses a dipeptide amino acid sequence with a piezoelectric effect, a dipeptide piezoelectric fiber film is prepared from the dipeptide amino acid sequence through a drop casting method, a meniscus driving method and an electric field induction meniscus driving method, and the obtained fiber film is assembled into an application example of a piezoelectric generator. The amino acid sequence of the dipeptide with the piezoelectric effect is Fc-L-Phe-L-Phe-OH, in the formula, Fc is a ferrocene benzoic acid group, Phe represents phenylalanine, and L represents the chirality of used amino acid. The dipeptide micron fiber thin film which is large in area and closely arranged is prepared by utilizing a drop casting method, a semilunar driving method and various methods of introducing a regulation and control electric field in a semilunar driving method membrane preparation process. The obtained fiber film is assembled into a piezoelectric generator of a sandwich structure, the piezoelectric output performance of the piezoelectric generator is closely related to the preparation method of the fiber film, and the piezoelectric performance of the dipeptide fiber film can be remarkably improved through an electric field induction meniscus driving method.

The present application provides a kind of 3-aminoisobutyric acid derivatives and preparation method thereof.The 3-aminoisobutyric acid derivatives of the present application are formed by 3-aminoisobutyric acid and arginine, and the preparation method comprises: step A: Boc-L-BAIBA is condensed with guanidyl-protected L-arginine under the action of condensing agent to obtain fully-protected dipeptide;Step B: the fully-protected dipeptide is reacted under the action of deprotecting agent to obtain 3-aminoisobutyric acid derivative.

The invention relates to the technical field of food processing safety and environmental pollution control, in particular to a natural compound preparation with a synergistic formaldehyde removal function and a preparation method and application thereof. The natural compound preparation is prepared from the following raw materials in percentage by mass: 5%-10% of carnosine, 0.5%-2% of epigallocatechin gallate, 0.05%-0.2% of edible essence, 10%-20% of a surfactant and the balance of a solvent, and the total percentage is 100%. The invention aims to reduce the exposure risk of specific groups such as chefs and guarantee the health level of the specific groups, the main component carnosine is dipeptide which can be used as a food additive and has good antioxidant activity, and amino in the structure of the carnosine can be subjected to Michael addition reaction with carbonyl of aldehydes. The epigallocatechin gallate is a common polyphenol substance, and phenolic hydroxyl groups in the epigallocatechin gallate can be reacted with aldehydes to capture and remove the aldehydes.

The invention provides a dipeptide capable of rapidly supplementing methionine and application thereof, and belongs to the field of nutritional and functional food. The problems of quickly supplementing methionine and promoting lactation are solved. The preparation method comprises the step of preparing the dipeptide GM of which the C terminal is methionine through solid-phase synthesis. The dipeptide GM can be used for supplementing methionine, and it is verified that compared with EM, KM and MM, the dipeptide GM has the higher methionine absorption rate in cells based on the PepT1 vector. The dipeptide GM can also be used for promoting lactation, and it is verified that the dipeptide GM has a better lactation promoting effect compared with EM, KM and MM.

The present invention relates to the use of a dipeptide comprising proline and alanine as a salt taste enhancer in a salt-containing consumable product, to a consumable composition comprising said dipeptide proline-alanine, and to a consumable product comprising said consumable composition. In addition, the present invention also relates to a method for enhancing salt taste and reducing salt content in food and beverages.

The invention discloses a diketopiperazine heterodimer as well as a biosynthesis method and application thereof, and belongs to the technical field of bioengineering. The biosynthesis method of the diketopiperazine heterodimer comprises the following steps: S1, culturing a recombinant cell containing P450 dimerization enzyme or a mutant thereof, and performing induced expression to obtain a whole-cell culture; and S2, adding a cyclic dipeptide substrate containing tryptophan into the whole-cell culture, carrying out a catalytic reaction, and separating and purifying the obtained product to obtain the diketopiperazine heterodimer. According to the invention, the P450 dimerization enzyme or the mutant thereof is used for catalyzing the biosynthesis of the tryptophan-containing diketopiperazine into the diketopiperazine heterodimer with different regioselectivity / stereoselectivity, the biosynthesis method is green and environment-friendly, the efficiency is high, and the variety of the diketopiperazine heterodimer is greatly expanded.

The invention provides an innovative dual-mode detection method based on a dipeptide nano framework (DNPs), which is used for efficiently detecting helicobacter pylori. According to the method, firstly, a DNPs material formed by chelating dipeptide (Trp-Phe) and zinc ions (Zn (II)) is synthesized, and the material is decomposed along with the increase of the pH value in an alkaline environment, so that the Zn (II) is released; then, urea is hydrolyzed under the catalysis of urease to generate ammonia, so that the pH value of the solution is increased, and the decomposition of DNPs and the release of Zn (II) are further promoted. By utilizing an electrochemical detection mode, real-time analysis on urea and urease is realized by monitoring an electrochemical signal of Zn (II); meanwhile, in combination with the fluorescence characteristics of DNPs, the concentration of urea and urease is indirectly and quantitatively analyzed by monitoring the fluorescence intensity change. The dual-mode detection method combines the advantages of electrochemistry and fluorescence detection, has high sensitivity and real-time performance, provides an effective means for accurate detection of helicobacter pylori, and shows a wide application prospect in clinical diagnosis and sensing technologies.

The application relates to the field of high polymer materials, in particular to an exosome composite material for treating periodontitis and a preparation method thereof. The exosome composite material comprises the following raw materials in parts by weight: 10-20 parts of a chitosan derivative, 10-20 parts of sodium hyaluronate, 1-5 parts of zein, 0.5-1 part of exosomes, 0.1-0.3 parts of phosphonodipeptide sodium and 60-70 parts of deionized water. The exosome composite material can inhibit periodontal pathogenic bacteria, reduce the expression of proinflammatory factors by inhibiting the signal pathways such as NF-kappa B, and thus relieve periodontal tissue inflammation.

The invention belongs to the technical field of biochemistry and functional polymer materials, and particularly relates to a polysaccharide-oligopeptide co-assembled intestinal flora regulation and control material and a preparation method thereof. The material is prepared from imine-boric acid ester double dynamic crosslinking modified chitosan, glycyl-tryptophan dipeptide, sodium lactate, citric acid, glycerol and a phosphate buffer solution. A reversible imine bond and a borate bond are introduced into chitosan molecules to construct a polysaccharide network with a double-dynamic crosslinking characteristic, and then the polysaccharide network and oligopeptide molecules are synergistically self-assembled to form a three-dimensional gel structure. The material has pH responsiveness, self-repairing property and slow-release function, and can realize continuous release of short peptides and selective regulation and control of probiotics in an intestinal environment. The prepared material is high in gel strength, good in structural stability and stable in oligopeptide release, and has remarkable promotion and inhibition selectivity on intestinal flora. The method provides an innovative co-assembly strategy for polysaccharide functionalization and micro-ecological intervention, and has a good application prospect.

The objective of this invention is to provide a highly safe, long-term-use, and excellent food-derived Netrin-1 production promoter. [Solution] White fungus exhibits excellent Netrin-1 production-promoting activity, and the combination of white fungus and imidazole dipeptide showed a particularly remarkable Netrin-1 production-promoting activity compared to white fungus and imidazole dipeptide alone. Therefore, white fungus, white fungus, and imidazole dipeptide can be used as foods, quasi-drugs, and pharmaceuticals with excellent Netrin-1 production-promoting activity.

The application discloses a Vibrio parahaemolyticus quorum sensing inhibiting peptide and application thereof, and belongs to the field of bioactive peptides. The inhibiting peptide comprises the following amino acid sequence: Ser-Phe; the application further discloses application of the quorum sensing inhibiting peptide in inhibiting Vibrio parahaemolyticus or preparing antibacterial drugs for inhibiting Vibrio parahaemolyticus. The application adopts a molecular docking technology to screen an active peptide having an inhibiting effect on a Vibrio parahaemolyticus LuxS / AI-2 quorum sensing system, ADMET property prediction is carried out on a peptide segment with better binding force, and finally, a bioactive peptide SF stably combined with LuxP, LuxQ and LuxS is obtained; the dipeptide has good in-vitro inhibiting activity on the Vibrio parahaemolyticus LuxS / AI-2 quorum sensing, and has no cytotoxicity. The dipeptide obtained by the application can be used for guiding development of a new type of antibacterial preparation applied to food, medicine and agriculture and other fields, and has great significance for human health and agricultural development.

The invention provides a method for producing L-carnosine, a genetic engineering strain as well as a preparation method and application of the genetic engineering strain, and belongs to the field of genetic engineering. According to the genetic engineering strain provided by the invention, by knocking out a glucose-6-phosphate isomerase coding gene pgi, knocking out an L-threonine / L-homoserine transporter coding gene rhtA or knocking out a dipeptide transfer protein coding gene dppABCD, the yield of extracellular L-carnosine is increased, the shake flask yield of the finally constructed strain reaches 9.1 g / L, the 5L fermentation tank yield reaches 65 g / L, and the yield of the extracellular L-carnosine reaches 9.1 g / L; and the method has a good application prospect in L-carnosine biosynthesis.

The invention discloses a method for synthesizing dipeptide Fmoc-Leu-Aib-OH, and belongs to the field of polypeptide synthesis, the specific preparation method comprises the following steps: under the action of organic alkali and an activating reagent, Fmoc-Leu-OH and H-Aib-OH react to obtain the dipeptide Fmoc-Leu-Aib-OH; the activating reagent comprises at least one of N, N, N ', N'-tetramethyl chloroformamidine hexafluorophosphate, 2-(7-azabenzotriazole)-N, N, N ', N'-tetramethyl urea hexafluorophosphate and benzotriazole-1-yl-oxytripyrrolidinyl phosphorus hexafluorophosphate, and the organic base comprises at least one of N-methylimidazole, N, N-diisopropylethylamine and pyridine. The dipeptide Fmoc-Leu-Aib-OH prepared by the preparation method disclosed by the invention is high in yield, high in purity and high in raw material conversion rate.

The invention belongs to the technical field of medicines, and particularly relates to a dipeptide capable of preventing and delaying colorectal cancer and application of the dipeptide. The invention provides a dipeptide for preventing and delaying colorectal cancer, which has the effects of inhibiting the activity and proliferation of colorectal cancer cells and can delay the development of colorectal cancer. And the product can be easily absorbed by human bodies, has the capability of supplementing essential amino acids for human bodies and a nutritional function, and has a good application prospect as a medicine for preventing and delaying development of colorectal cancer.

The invention discloses staphylococcus warneri CCSM005 separated from human skin and capable of improving skin health and a metagen thereof, and belongs to the technical field of microorganisms and medicines. The staphylococcus warneri CCSM005 provided by the invention can be fermented to produce the L-glycine-valine dipeptide, and a fermentation supernatant of the staphylococcus warneri CCSM005 has a good effect of repairing a skin barrier function, which is specifically shown in that the activity of damaged HaCaT cells is improved in vitro, and the gene level expression of FLG, ZO-1, CLDN and Occludin can be improved at the same time after the damage. Therefore, the staphylococcus warneri CCSM005 has a huge application prospect in preparation of external daily chemical products or medicines for repairing the skin barrier.

The invention relates to the technical field of cosmetics, in particular to a compound active peptide with skin anti-aging, anti-inflammatory and soothing functions and application of the compound active peptide. The compound active peptide is prepared from acetyl hexapeptide-1, palmitoyl tripeptide-5, acetyl dipeptide-1 cetyl ester, copper peptide and decarboxylated carnosine hydrochloride. The compound peptide acts on dermal fibroblasts, so that expression of p16 and p21 genes is reduced, and cell cycle arrest is improved; the compound acts on epidermal keratinocytes and dermal fibroblasts and inhibits COX-2 gene expression; the compound peptide acts on epidermal keratinocytes and dermal fibroblasts, and the ROS level is reduced; the compound peptide acts on PC12 cells, inhibits secretion of acetylcholine, blocks transmission of neural signals, and plays a role in immediate wrinkle removal.

The application discloses an anti-inflammatory soothing repair polypeptide composition containing tripeptide-125 and a preparation method and application thereof, and relates to the technical field of cosmetic polypeptides. The efficacy components of the composition comprise palmitoyl tripeptide-8, acetyl dipeptide-1 cetylester and tripeptide-125. The anti-inflammatory soothing repair polypeptide composition is prepared by using the efficacy components, a solvent, a solubilizer and a bacteriostatic agent, and has good anti-inflammatory, soothing and skin barrier repair performance.

The invention discloses a leucine-containing dipeptide mixture easy to dissolve and reduced in bitterness as well as a preparation method and application of the leucine-containing dipeptide mixture. The mixture comprises the following components in percentage by mass: not less than 90% of alanyl-leucine, 0-5% of free leucine, less than 1% of water and not more than 0.10% of other impurities. The solubility of the mixture at room temperature reaches up to 500 g / L, the mixture is kept transparent and free of precipitates, the bitterness is reduced by 65% or above compared with leucine, the taste can be improved without a taste masking agent, and the mixture is suitable for sports nourishment and functional food. According to the preparation method, an enzyme catalysis reaction is adopted, and the steps of solid-liquid separation, pH adjustment, crystallization, nanofiltration concentration, drying and the like are performed, so that the total yield is not lower than 90%, the purity is not lower than 99.5%, and high solubility, low bitter taste, instant solubility and environmental friendliness are achieved; and the method has a wide industrial application scene.

The invention belongs to the field of preparation of polypeptide drugs, and discloses a synthesis method of tilpotide, which mainly comprises the following steps: 1) taking amino resin as initial resin, and coupling with protected amino acid and polypeptide fragments by adopting a solid-phase synthesis method to obtain peptide resin of tilpotide; wherein the Glu3-Gly4 adopts a Glu-Gly dipeptide fragment, the Thr5-Phe6 adopts a Thr-Phe dipeptide fragment, the Lys20 adopts a Lys [AEA-AEA-gamma-Glu-C20] fragment, and the Gly29-Gly30 adopts a Gly-Gly dipeptide fragment; 2) cracking the peptide resin to obtain crude peptide of the tilpotide; and (3) purifying the crude peptide by reversed-phase chromatography to obtain the fine peptide of the tilpotide. The invention provides a synthesis method of telpotide, which can effectively reduce the generation of [D-Glu] racemization impurities, [D-Thr] racemization impurities, [D-Phe] racemization impurities and [+ Gly] impurities, thereby enhancing the purity and yield of the telpotide, obviously lowering the difficulty of purifying crude peptide, greatly enhancing the total yield of the telpotide on the premise of ensuring the purity of the telpotide, and lowering the production cost of the telpotide. In addition, synthesis of a plurality of fragments can be performed simultaneously, so that the synthesis time is shortened. The purity of the crude tirpotide prepared by the method can reach 86.22% or above, and the yield of the crude tirpotide is 101.51% or above. Through simple purification steps, the impurities in the tilpotide are basically removed, the purity of refined peptide can reach 99.21% or above, the maximum single impurity is lower than 0.15%, the total yield can reach 63.02% or above, the synthesis cost is reduced, and industrial mass production is facilitated.

The invention discloses a phage endolysin prediction method and system based on machine learning, a storage medium and electronic equipment, and belongs to the technical field of bioinformatics. The method comprises the following steps: acquiring a phage protein sequence and performing pretreatment; extracting features by combining six methods of amino acid entropy, amino acid index, binary position coding, composition-conversion-distribution, dipeptide composition and gene triad composition; performing cross validation training by using five base model groups including a random forest, logistic regression, a decision tree, K neighbor and a gradient boosting tree, and constructing a support vector machine meta-model to complete construction of a stacking integration model; and processing a to-be-predicted sequence and inputting the processed sequence into the model to obtain a prediction result. According to the method, through multi-feature combination and a specific stacking model structure, the prediction precision is remarkably improved, the accuracy on an independent test set reaches 96.4%, and the problems that a traditional experimental method is long in period and high in cost and an existing calculation method is insufficient in precision are effectively solved.

The invention discloses a peptide with an anti-photoaging effect as well as a preparation method and application of the peptide. The cyclic dipeptide cyclo (LO) and the linear dipeptide LO disclosed by the invention can be used for remarkably inhibiting the activity of MMP-1 and efficiently blocking collagen degradation. In addition, the cyclic dipeptide and the linear dipeptide have no cytotoxic effect when being co-cultured with fibroblasts, so that the cyclic dipeptide and the linear dipeptide have no stimulation and side effects on cells, and are high in safety and good in stability. The cyclic dipeptide and the linear dipeptide disclosed by the invention can resist UVA (Ultraviolet A) damage, have a promoting effect on cell proliferation activity after UVA irradiation and have an anti-aging effect on cells after UVA irradiation. The two peptides disclosed by the invention can be used for preparing medicines or cosmetics for resisting light aging, resisting aging, beautifying and protecting skin. The peptide fragment disclosed by the invention has a good application prospect in the fields of medicines, cosmetics and the like.

The invention discloses a moisturizing and soothing cosmetic containing an artemisia apiacea extract and a preparation method of the moisturizing and soothing cosmetic. The cosmetic is prepared from the following raw materials in percentage by weight: 0.5%-2% of 1, 2-hexanediol, 0.3%-1% of p-hydroxyacetophenone, 5%-15% of butanediol, 0.1%-1% of dimethyl sulfone, 0.3%-2% of zanthoxylum armatum extract, 0.2%-1.5% of aesculus chinensis extract, 0.1%-0.5% of dipotassium glycyrrhizinate, 0.5%-3% of panthenol, 0.1%-0.8% of allantoin, 0.1%-1% of oat beta-glucan, 0.05%-0.5% of hydrolyzed sodium hyaluronate, 0.001%-0.01% of caramel color, 0.1%-1% of cyclodextrin, 0.05%-0.3% of acetyl dipeptide-1 cetyl ester, 0.5%-2.5% of scutellaria baicalensis extract, 0.3%-2% of purslane extract and 0.2%-1.5% of a chamomile extract and the balance of deionized water. The traditional Chinese medicine composition has the advantages that various extracts are in synergistic interaction; the safety is high; the composition is suitable for sensitive skin, dry skin and other skin types, and can be prepared into emulsion, face cream, essence and other dosage forms.