635 results about "Dipeptide" patented technology

The present invention belongs to the field of biopharmaceutical technology, and specifically relates to a method for preparing GLP-1 or its analogs. The present invention provides a leader peptide, and a fusion protein comprising the leader peptide. The leader peptide or fusion protein is used to prepare a semaglutide precursor polypeptide, and the results show that the yield of the prokaryotic expression fusion protein can reach 26g / L, and the purity of the precursor polypeptide obtained after enzyme cleavage is higher than 95%. The precursor polypeptide is further side-chain modified and connected with a dipeptide to efficiently prepare semaglutide.

The invention relates to the technical field of preparation methods of polypeptide drugs, and provides a solid-phase preparation method of tirpotide, which comprises the following steps: by taking amino resin as initial resin, sequentially coupling amino acid and small-fragment peptide according to an amino acid sequence of the tirpotide by using a solid-phase synthesis method to synthesize tirpotide resin; and cracking and purifying the tilpotide resin to obtain a pure tilpotide product. Wherein the small fragment peptide is 1-4 tetrapeptide fragment Boc-Tyr (tBu)-Aib-Glu (OtBu)-G1y-OH, 5-6 dipeptide fragment Fmoc-Thr (tBu)-Phe-OH, 7-8 dipeptide fragment Fmoc-Thr (tBu)-Ser (tBu)-OH, 10-11 dipeptide fragment Fmoc-Tyr (tBu)-Ser (tBu)-OH, 12-13 dipeptide fragment Fmoc-Ile-Aib-OH, 17-18 dipeptide fragment Fmoc-Lys (AEEA-AEA-gamma-Glu (alpha-OtBu)-Ecosanoic acid (mon-tBu), 20 side chain peptide Fmoc The coupling agent coupling the amino acid and the small fragment peptide is selected from the group consisting of Oxyma and DIC, TPTU and TMP or COMU and DIEA. The crude product of the tirpotide prepared by the method disclosed by the invention is high in yield and high in purity.

The invention belongs to the field of skin care products, and particularly relates to an anti-wrinkle and anti-aging composition as well as a preparation method and application thereof, and the anti-wrinkle and anti-aging composition comprises neurotransmitter inhibition peptide, signal peptide, madecassoside, inositol and glycosylglycerol. The neurotransmitter inhibition type peptide is at least three of acetyl hexapeptide-8, arginine / lysine polypeptide, dipeptide diaminobutyrylbenzylamide diacetate and acetyl octapeptide-3, and the signal type peptide is at least two of palmitoyl pentapeptide-4, decapeptide-4 and acetyl tetrapeptide-9. Compared with the prior art, all the components of the anti-wrinkle and anti-aging composition act on different anti-aging pathways and can be matched with one another from multiple aspects of oxidation resistance, inflammation resistance, moisturizing, cell viability enhancement, wrinkle reduction and the like, a more comprehensive and better anti-aging effect is provided, skin aging is comprehensively resisted, the skin is in a more young state, and the anti-wrinkle and anti-aging composition has the advantages that the anti-wrinkle and anti-aging effects are achieved. And the anti-wrinkle and anti-aging composition is small in irritation and good in stability.

The invention provides anti-inflammatory soothing application of cyclic dipeptide. The cyclic dipeptide is selected from cyclic (L-leucine-L-proline) dipeptide, cyclic (D-leucine-L-proline) dipeptide or a combination of the cyclic (L-leucine-L-proline) dipeptide and the cyclic (D-leucine-L-proline) dipeptide. The anti-inflammatory soothing of the present invention is achieved by inhibiting TNF [alpha], IL-1[ beta], IL-6, PGE2, COX2, TSLP and / or IL-33. The invention also relates to an application of the cyclic dipeptide in preparation of external skin agents, medicines and health-care foods with anti-inflammatory and soothing effects. The external skin agents are selected from face cream, emulsion, gel, toning lotion, essence, masks, eye cream, aerosol cleaning foam, spray, shower gel or facial cleanser.

The invention discloses a one-pot preparation method of semeglutide, and relates to the field of polypeptide synthesis. The preparation method comprises the following specific steps: 1) taking Arg34GLP-1 (9-37) as a starting material, controlling the pH value of a reaction solution, enabling activated dipeptide Fmoc-His-Aib-OH to react with the starting material to obtain an acylation reaction solution, and directly carrying out next-step reaction without aftertreatment after the reaction is finished; 2) adjusting the pH value of the acylation reaction liquid I, adding a compound 2-tetrapeptide into the acylation reaction liquid I, reacting to obtain acylation reaction liquid II, and directly carrying out next-step reaction without post-treatment after the reaction is finished; (3) adding an alkaline reagent to remove the Fmoc protecting group, settling, centrifuging and drying to obtain a crude product of the semeglutide; 4) separating and purifying the crude product to obtain a refined semeglutide product; the first three steps are carried out in the same reactor. Compared with a conventional liquid-phase synthesis method, the preparation method disclosed by the invention is simpler and more convenient, short in construction period, low in output of waste liquid and waste water, greatly reduced in cost, higher in yield and suitable for large-scale production.

The invention relates to a composite peptide with anti-inflammatory and soothing effects, a composition and application thereof, and belongs to the technical field of polypeptides. The invention relates to a composite peptide with anti-inflammatory and soothing effects. The composite peptide is prepared from the following components: tripeptide-1, acetyl dipeptide-1, palmitoyl tetrapeptide-7 and palmitoyl tripeptide-8, the mass ratio of the tripeptide-1 to the acetyl dipeptide-1 to the palmitoyl tetrapeptide-7 to the palmitoyl tripeptide-8 is (0.5 to 1.5) to (0.6 to 1.5) to (1 to 2) to (0.5 to 1.5). Experiments prove that the composite peptide has good anti-inflammatory, soothing and anti-wrinkle effects.

The invention provides an AS01 derivative adjuvant, a preparation method thereof and application of the AS01 derivative adjuvant in preparation of vaccines or anti-tumor drugs, and belongs to the technical field of biological drugs or products. The AS01 derivative adjuvant is prepared from the following components in parts by mass: 0.5 to 1.0 part of cholesterol, 2.0 to 4.0 parts of dioleoyl lecithin and at least one of the following components in parts by mass: 0.1 to 0.2 part of monophosphoryl lipid A, 0.1 to 0.2 part of QS-21, 0.36 to 0.72 part of 3M-052, 0.4 to 0.8 part of muramyl dipeptide and 0.2 to 0.4 part of oligodeoxynucleotide. According to the AS01 derived adjuvant, a novel synergistic adjuvant system is developed by integrating various immunomodulators, so that the immunogenicity of a vaccine is enhanced. The HPV vaccine is prepared on the basis of the AS01 derived adjuvant, so that the immunogenicity can be enhanced, and good anti-tumor activity can be achieved. Therefore, the invention provides a new strategy for immunotherapy of HPV-related tumors.

The present disclosure relates generally to compositions and methods for inhibiting dipeptide repeat protein (DPR)-ribosomal RNA (rRNA) interaction. In particular, the present technology relates to administering a therapeutically effective amount of one or more compositions that inhibit DPR-rRNA interaction to a subject diagnosed with, or at risk for DPR-associated pathologies, e.g., amyotrophic lateral sclerosis or frontotemporal dementia.

The present invention relates to the field of medical technology, and in particular to a semaglutide intermediate and a preparation method thereof, and a method for synthesizing semaglutide. The present invention screens different solvent combinations, finds a reaction system compatible with side chain tetrapeptide modification, main chain dipeptide modification and deprotection steps, and synthesizes semaglutide using a "one-pot method". The method does not require separation of any intermediate, does not involve complex operations such as isoelectric elution, separation, and redissolution of the intermediate, is simple to operate, has a high yield, and is conducive to industrial scale-up production. Further, the present invention uses a double Fmoc-protected dipeptide: Fmoc-His(Fmoc)-Aib-OH replaces Fmoc-His-Aib-OH·TFA as a starting material, which greatly reduces the use of Fmoc-His(Fmoc)-Aib-OH, and the feed amount is reduced from 8 to 10 times to 1 to 3 times, the atomic economy is increased, and the production cost is reduced.

Provided are an antibacterial peptide and the use thereof. The antibacterial peptide has a structure represented by formula (I), wherein Z1 is selected from tetrapeptides, the tetrapeptides being optionally modified by -OH, -CHO, -COOH, -NH2, -SO3H or -C=O; S1 and S2 are each independently selected from amino acids having the structure of (I); Q1 and Q2 are each independently selected from dipeptides or tripeptides; and n is independently selected from any integer between 1 and 6. (Q2)4-(S2-Q1)2-S1-Z1

The invention provides a composition with an instant and long-acting anti-aging effect and application thereof. The composition comprises a component A, a component B and a component C, the mass ratio of the raw materials is (1-10): (1-4): (0.05-0.2); the component A is prepared from the following components in parts by weight: 0.01 to 0.5 part of acetyl octapeptide-3, 0.01 to 0.5 part of dipeptide diaminobutyryl benzyl amide diacetate, 0.001 to 0.2 part of arginine / lysine polypeptide, 0.3 to 1 part of caprylyl glycol and 96 to 100 parts of water; the component B is prepared from the following components in parts by weight: 0.001 to 0.2 part of soluble collagen, 5 to 30 parts of glycerol, 0.01 to 0.5 part of sodium dihydrogen phosphate, 0.001 to 0.2 part of disodium hydrogen phosphate and 65 to 100 parts of water; and the component C is tripeptide-29. According to the invention, the quadruple wrinkle fading polypeptide is adopted to block wrinkles in a multi-target full-link manner, so that the product has a very good effect of fading various wrinkles in real time.

The invention discloses a deep learning-based umami peptide prediction method and system. The method comprises the following steps of S1, constructing a data set; s2, feature selection and processing; s3, constructing a Umami-Transform model: constructing the Umami-Transform model which comprises a feature processing and generating module, a sequence information processing module, a feature information processing module and a result output module; s4, model training; and S5, umami peptide prediction: inputting a peptide sequence to be detected into the trained Umami-Transformer model to obtain a probability prediction result that the peptide sequence is the umami peptide. According to the method, the Transform architecture and eight key physical and chemical characteristics are fused, so that peptide sequence information can be efficiently processed, and the prediction precision of the umami peptide is remarkably improved. All dipeptides to pentapeptides are screened by further combining with an exhaustion method, and an interaction mechanism between candidate peptides and the umami receptor T1R1 / T1R3 is analyzed through a molecular docking technology, so that the whole-process research from theoretical prediction to biological experimental verification is finally realized, and an innovative technical path is provided for efficient excavation and functional application of the umami peptides.

The invention discloses a solid-state flash-release acne-removing composition and an application thereof in a soluble microneedle, and particularly belongs to the technical field of cosmetics. The acne-removing composition is prepared from the following components in parts by mass: 0.5 to 1 part of acne-removing functional component, 4 to 5 parts of hyaluronic acid and 1 to 2 parts of carboxymethyl cellulose, the acne-removing functional component contains a spruce extract, a yeast / zinc fermentation product and dipeptide-15, and the spruce extract is prepared through supercritical extraction. The soluble microneedle containing the acne-removing composition is prepared by utilizing a soluble microneedle preparation technology, and the effects of quickly resisting inflammation, inhibiting bacteria and quickly transdermal repairing can be achieved.

The invention relates to a transdermal dipeptide and application thereof, and belongs to the technical field of active polypeptides. A novel transdermal dipeptide Arg-Pro is designed and synthesized, the transdermal dipeptide has obvious penetration enhancing capacity, the penetration enhancing effect of the transdermal dipeptide is better than that of existing penetration enhancers, namely transdermal peptide TD-1, acetyl hexapeptide-1 and menthol, and the transdermal dipeptide is simple in structure, small in molecular weight, high in biocompatibility and free of toxic effects on cells, can be used for preparing penetration enhancing products and has good application prospects. Wide development prospects and application potentials are realized. Meanwhile, transdermal dipeptides Lys-Pro and Asn-Pro are also synthesized, and the transdermal delivery effect of the transdermal dipeptides Lys-Pro and Asn-Pro is slightly lower than that of Arg-Pro, but the transdermal dipeptides Lys-Pro and Asn-Pro still have an obvious penetration promoting effect.

The present invention relates to the fields of medicine and cosmetology, and discloses a cell active peptide composition that promotes the regeneration of multi-type collagen structural proteins, its preparation method, and its application in medicine and cosmetology. The composition uses raw materials such as tripeptide-10 citrulline, hexapeptide-9, hydrolyzed sodium hyaluronate, γ-aminobutyric acid, decarboxylated carnosine hydrochloride, acetyl tetrapeptide-3, acetyl tetrapeptide-11, palmitoyl dipeptide-7, and palmitoyl pentapeptide-4. The composition can promote the coordinated expression of skin type I, type III, type IV, type VII, type XVII collagen, fibronectin, elastin, and glycosaminoglycans; prevent collagen cross-linking, maintain triple helix structure, and inhibit collagen degradation. Applied to dermatology and cosmetology, it can accelerate the regeneration of structural proteins such as skin collagen, repair skin damage, reduce wrinkles such as crow's feet, tear groove lines, nasolabial folds, and under-eye lines, solve the skin aging problem caused by the loss of structural proteins such as collagen, and improve skin elasticity and firmness.

Provided are a preparation method for alpha-amino acid ester acyl transferase, and a use thereof in dipeptide synthesis. A method for dipeptide synthesis comprises: using alpha-amino acid ester acyl transferase to perform a dipeptide synthesis reaction, to obtain a dipeptide; the alpha-amino acid ester acyl transferase has a conserved region with an amino acid sequence as shown in SEQ ID NO: 1. Using the alpha-amino acid ester acyl transferase, a variety of functional dipeptides can be synthesized, a substrate spectrum is wide, and the synthesis efficiency of some enzymes is high; this can be well used for industrial amplification, with low cost and high yield, thus realizing green chemistry.

The invention discloses a method for synthesizing a high-purity pseudo proline dipeptide heterocyclic ring. The method comprises the following steps: (1) preparing L-serine or L-threonine into corresponding methyl ester hydrochloride; (2) condensing the methyl ester hydrochloride obtained in the step (1) with a condensation reagent to form a corresponding pseudo proline ring; and (3) condensing the pseudo-proline ring formed in the step (2) and related amino acid protected by Fmoc to obtain the corresponding pseudo-proline dipeptide heterocyclic ring. The total yield of the target product synthesized by the reaction can reach more than 80%. The method solves the problems of long reaction time, low yield, high safety risk and the like in the reported route, and has the advantages of cheap and easily available raw materials, lower production cost, simplicity in operation, environmental friendliness, high yield, short reaction time, simplicity in post-treatment and the like.

The present invention discloses a recombinant Escherichia coli producing α-amino acid ester acyltransferase, a construction method and an application thereof, and belongs to the field of biotechnology. The recombinant Escherichia coli is deposited in the General Microbiological Center of the China Microbiological Culture Collection Administration Committee, with a deposit address of No. 3, Yard No. 1, Beichen West Road, Chaoyang District, Beijing, a deposit date of March 22, 2024, and a deposit number of CGMCC No. 30105. The recombinant Escherichia coli constructed by the present invention can produce α-amino acid ester acyltransferase, and the enzyme is used to catalyze the synthesis of glutathione, with a conversion rate of up to 82.59%, which is of great significance to the industrial development of biosynthesis of glutathione.

The invention discloses a cyclic dipeptide precursor aroma component as well as a preparation method and application thereof, the high-content cyclic dipeptide precursor aroma component, especially cyclic (proline-valine) dipeptide, cyclic (proline-isoleucine) dipeptide and cyclic (proline-leucine) dipeptide, is produced through bacillus velezensis, and the cyclic (proline-valine) dipeptide, the cyclic (proline-isoleucine) dipeptide and the cyclic (proline-leucine) dipeptide are used as the precursor aroma component of the cyclic dipeptide. The cyclic dipeptide precursor aroma components and the fermentation liquor biological perfume can significantly improve the milk aroma and baking aroma of cigarettes, and enhance the corresponding aroma characteristics of the cigar tobacco leaves, so that the overall quality of the cigar tobacco leaves is improved. In addition, the cyclic dipeptide precursor aroma component generated by the bacillus velezensis and the fermentation liquor biological perfume provide a new way for improving the quality of tobacco products; the application of the components not only can enrich the sensory experience of tobacco products, but also opens up a new direction for product innovation and quality improvement in the tobacco industry.

The invention relates to an anti-wrinkle and anti-aging composition and application thereof. The anti-wrinkle and anti-aging composition is prepared from hydrolyzed rice protein, a bifidus yeast fermentation product lysate, dipeptide diaminobutyryl benzyl amide diacetate, adenosine, hydroxydecyl ubiquinone, ceramide NP, palmitoyl tripeptide-5, carnosine and acetyl hexapeptide-8. According to the present invention, the components achieve the synergistic effect, such that the fibroblast proliferation can be promoted, the cell metabolism can be improved, the generation of collagen, elastin and the like can be promoted, the excellent oxidation resistance can be provided, and the effects of expression line inhibition, existing wrinkle fading and long-term aging resistance can be combined.

Provided is a method for simply and quickly screening for proteins associated with neurodegenerative diseases, or compounds that are useful for the treatment or prevention of neurodegenerative diseases. The method uses a probe formed by covalently bonding an environmentally responsive fluorophore to a dipeptide repeat sequence comprising a repeating unit selected from the group consisting of proline-arginine, glycine-arginine, proline-alanine, glycine-alanine and glycine-proline.

The present application relates to a kind of dipeptide derivative compositions and its preparation method and purposes, the composition includes (a) formula I compound, (b) glycine and (c) antioxidant, the composition has good adjuvant compatibility, the impurity content of lyophilized preparation prepared according to the composition is low, under high humidity, strong light and low temperature storage condition, all have good stability.

The present invention discloses a cosmetic composition with the effects of oil control and pore refinement, and a cosmetic prepared mainly with the cosmetic composition. In terms of weight percentage, the cosmetic composition of the present invention comprises 0.1-2% of dipeptide-15, 0.05-3% of butyl avocado oleate, 0.1-1% of hydroxystearic acid, and 0.01-2% of chlorella extract, but does not comprise acid-cleaning ingredients such as fruit acid and salicylic acid. The cosmetic composition has the effects of controlling the oil content of the skin and refining the pores, and the components synergize to enhance the effect, and can achieve a faster and more significant effect under an appropriate compounding ratio.

The invention discloses a bipeptide modified bionic nano-vesicle as well as a preparation method and application thereof, and belongs to the field of biological medicines. The dipeptide modified bionic nano-vesicle comprises nano-particles formed by PLGA (poly (lactic-co-glycolic acid)), and the nano-particles are loaded with a medicine with a nerve protection or nerve repair effect; the surface of the nanoparticle is coated with a macrophage membrane for expressing RVG peptide and T7 peptide. The bipeptide modified bionic nano-vesicle simultaneously presents T7 peptide and RVG peptide through an engineered macrophage membrane, the T7 peptide is combined with a blood-brain barrier transferrin receptor through high affinity to realize efficient brain entry, astrocytes in the brain are specifically recognized by virtue of the RVG peptide, accurate recognition and delivery of target cells in a focus area are realized, and the bipeptide modified bionic nano-vesicle has a good application prospect. Meanwhile, the natural inflammation tropism and immune escape ability of a macrophage membrane are reserved, and the problems that a traditional drug delivery system is low in targeting precision, and cross-barrier distribution and intracerebral distribution are difficult to cooperate are solved.

The embodiment of the invention discloses a composition with an anti-UVB damage function, a skin care product and application. The composition and the skin care product can be used for solving the anti-UVB damage problem. The composition comprises: a first compound selected from a compound represented by a formula (I) or a formula (II); and a second compound, wherein the second compound is selected from any one of palmitoyl tripeptide-5, acetyl dipeptide-1 cetyl ester, acetyl tripeptide-30 citrulline, and palmitoyl pentapeptide-4.

The invention discloses a fermented caffeine eye cream composition. Comprising the following components in parts by weight: 30 to 80 parts of a fermentation composition, 1 to 5 parts of caffeine, 0.1 to 0.3 part of hydroxypropyl tetrahydropyrantriol, 0.5 to 2 parts of nicotinamide, 0.1 to 1 part of squalane, 0.01 to 0.5 part of a ceramide composition, 0.005 to 0.05 part of dipeptide diaminobutyryl benzyl amide diacetate, 0.005 to 0.05 part of a polypeptide composition, 0.01 to 0.5 part of carnosine, 0.01 to 0.5 part of tetrahydromethylpyrimidine carboxylic acid and 3 to 12 parts of a plant extract. The invention also discloses an eye cream containing the eye cream composition. The fermented caffeine eye cream composition provided by the invention can effectively smooth the skin surface, and is beneficial to preventing and comforting aging, improving skin elasticity, improving dark circles, relieving edema and removing under-eye puffiness.

The present invention relates to a compound binding to an endogenous receptor, said compound comprising (i) an oligopeptide comprising a dipeptide with Trp being the C-terminal amino acid of said dipeptide, wherein said Trp is replaced with an α-amino acid Xaa2, whereby the stability in serum or plasma of the peptide bond connecting Xaa2 to the N-terminally adjacent amino acid is increased as compared to the peptide bond connecting Trp to the N-terminally adjacent amino acid in an otherwise identical compound; and (ii) a moiety capable of generating therapeutically effective radiation, said moiety being covalently bound to said oligopeptide.

The invention relates to the technical field of skin care products. The invention aims to provide a split storage type novel skin-brightening skin-care product combined by oligopeptide freeze-dried powder and essence. The split storage type novel skin-brightening skin-care product comprises multiple oligopeptide freeze-dried powder and solvent essence, the multi-oligopeptide freeze-dried powder is prepared from the following raw materials in parts by weight: 90 to 95 parts of water, 1 to 2 parts of glutathione, 1 to 2 parts of mannitol, 1 to 2 parts of carnosine, 0.5 to 1 part of ergothioneine, 0.5 to 1 part of vitamin C and 0.0005 to 0.001 part of a multi-peptide-zinc chelate. The multi-peptide-zinc chelate is prepared from the following raw materials in parts by weight: 1-2 parts of hexapeptide-2, 1-2 parts of nonapeptide-1, 1-2 parts of dipeptide-2, 1-2 parts of tripeptide-10 and 2-3 parts of zinc oxide. According to the invention, various peptides with small molecular weights are chelated with zinc, the characteristics of good permeability and strong skin affinity of the small molecular peptides are fully played, the effects of zinc and other components are effectively played, and the overall use effect is greatly improved. Through compounding of multiple components, the moisturizing and brightening mask has multiple effects of moisturizing, good permeability, whitening, brightening and the like.

The invention provides a preparation method of dipeptide-2, which comprises the following specific steps: carrying out amino protection on L-valine by using 2-(trimethylsilyl) ethoxycarbonyl, carrying out condensation reaction on the product and L-tryptophan benzyl ester, carrying out deprotection to remove the 2-(trimethylsilyl) ethoxycarbonyl, removing benzyl under a palladium-carbon hydrogenation condition, and purifying to obtain the dipeptide-2. Belongs to the technical field of organic synthesis. According to the method, 2-(trimethylsilyl) ethoxycarbonyl is adopted as an amino acid protecting group, amino groups can be accurately and specifically protected, the operation steps of protecting the amino acids are simplified, conditions are simple to control and easy to implement, meanwhile, a dipeptide intermediate is generated through a condensation reaction, and a good product foundation is laid for subsequent preparation steps of dipeptide-2; in addition, the process reaction is rapid, the operation steps are simple and clear, the used reagents are easy to purchase and low in price, the method is very suitable for industrial large-scale production, and a new synthesis way is provided for preparation of the dipeptide-2.

The invention discloses dipeptide hydrogel and application thereof as a cell culture medium, and belongs to the field of material science. According to the invention, the dipeptide hydrogel is designed and assembled, and the dipeptide hydrogel is prepared by the following steps: carrying out self-assembly on phenylalanine dipeptide modified by 9-fluorenyl methoxy carbonyl protecting group (Fmoc), and then adding tetracarboxyl phenyl porphyrin (TCPP) and distearoyl phosphatidyl ethanolamine (DSPE) for co-assembly. When the dipeptide hydrogel prepared by the invention is applied as a cell culture medium, the proliferation activity of cultured cells can be improved, so that the growth speed and density of the cells are improved, and the dipeptide hydrogel has good biological safety. The possibility is provided for promoting the high-level development of the cell culture field.