Product
Patsnap Eureka
For R&D, Patsnap Eureka makes reading and utilizing patents & technical documents easy.
Patsnap Eureka AIR
Designed for self-driven R&D workflows. Generate viable solutions, solve complex R&D challenges, empower your innovation with AI.
Patsnap Eureka Materials
Designed for material experts only. Revolutionize your material R&D, from search, analyze, to developing new materials.
Feature
TechResearch
Generate reliable direction feasibility study reports for your R&D in just a few steps.
TechSeek
Discover and master advanced knowledge NOW. Basics, ideas, possibilities, all at once.
TechMind
As an expert in R&D Theories, TechMind can generates customized viable solutions instantly.
TechRisk
Analyze your overall solution with one click, know your potential R&D risks in advance.
TechMonitor
Get weekly tech updates, stay abreast of the latest tech innovations and key insights.
Patents
Literature
Hiro is an intelligent assistant for R&D personnel, combined with Patent DNA, to facilitate innovative research.
33 results about "Folate Metabolism" patented technology
Efficacy Topic
Property
Owner
Technical Advancement
Application Domain
Technology Topic
Technology Field Word
Patent Country/Region
Patent Type
Patent Status
Application Year
Inventor
Summary Folate is a generic term referring to both natural folates in food and folic acid, the synthetic form used in supplements and fortified food. Folate is critical in the metabolism of nucleic acid precursors and several amino acids, as well as in methylation reactions.
Kit for genotyping folate metabolism gene
ActiveCN104131087AIncreased sensitivityQuick checkMicrobiological testing/measurementHuman DNA sequencingNucleotide
The invention provides a kit for genotyping folate metabolism gene. The kit includes a PCR reaction liquid and a DNA hybrid membrane strip, the DNA hybrid film strip comprises a matrix vector and probes, four probes for MTHFR genes, two probes for MTRR genes and two probes designed for human genome are sequentially fixed on the matrix vector, each of the probes is an oligonucleotide sequence hybridized with the SNP locus of each of the corresponding gene, and the nucleotide sequences of the probes are represented by SEQ ID NO:1-8. The kit provided by the invention has the advantages of high sensitivity, rapid detection, good stability, realization of high or low flux detection, high flexibility, strong maneuverability for small clinical samples in some hospitals, less equipment investment and low cost.
Owner:WUHAN CMLABS CO LTD
Application, detection method and kit of MTHFR (Methylene Tetrahydrofolate Reductase) gene SNP (Single Nucleotide Polymorphism) guide folic acid and related nutrient element intake
InactiveCN101864479ATimely and accurate nutritional intake guidancePrevent physical defectsMicrobiological testing/measurementFolate MetabolismPhysiology
The invention discloses application, a detection method and a kit of SNP of an METHFR gene guiding folic acid and related nutrient element intake. Because of providing different positive locuses of SNP gene types of folic acid metabolic capacity and related nutrient element intake, the reason generating impediment thereof is found, and the guidance for guiding individuals, especially pre and post natal women, to take folic acid and intake related nutrients is provided.
Owner:吴奇涵 +2
Gene sequence variances in genes related to folate metabolism having utility in determining the treatment of disease
InactiveUS20050191691A1Affects efficacyEffective treatmentMicrobiological testing/measurementFolate MetabolismPyrimidine transport
The present disclosure describes the use of genetic variance information for folate transport or metabolism genes or pyrimidine transport or metabolism genes in the selection of effective methods of treatment of a disease or condition. The variance information is indicative of the expected response of a patient to a method of treatment. Methods of determining relevant variance information and additional methods of using such variance information are also described.
Owner:BODYSYNC
Detection method of folate metabolism related gene
InactiveCN107254520AReduced activityLow costMicrobiological testing/measurementGel electrophoresisRelated gene
The invention discloses a detection method of a folate metabolism related gene, which includes steps of extracting DNA from oral mucosa cell of a detector; amplifying MTHFR gene C677T by SEQ NO 1 and SEQ NO 2; amplifying MTHFR gene A1298C by SEQ NO 3 and SEQ NO 4; amplifying MTHFR gene A66G by SEQ NO 5 and SEQ NO 6; extracting DNA from oral mucosa cell as a template to perform PCR reaction; performing sepharose gel electrophoresis of 1% of ordinary PCR amplified product on three pairs of primers; recycling a strip of a kit recycling item from the 1% of agarose gel electrophoretic band by sepharose gel; performing sequence testing reaction; purifying the sequence testing reaction product and denaturing; testing sequence by an upper 3730 sequence tester; analyzing three SNP gene models of the sequence testing result by Chromas software. The method has the advantages of high detection accuracy, and convenience of use.
Owner:安徽安龙基因科技有限公司
Kit for detecting individual folate metabolism disorder
InactiveCN103667435AMicrobiological testing/measurementFolate Metabolism(Methionine synthase) reductase
The invention discloses a kit for detecting an individual folate metabolism disorder. The kit comprises a specific primer pair and SYBRGreenI fluorochrome for simultaneously detecting rs1801133# SNP site on a 5,10-methylene tetrahydrofolate reductase gene, rs1801131# SNP on a 5,10-methylene tetrahydrofolate reductase gene and rs1801394# SNP site on a methionine synthase reductase gene, a conventional component for real-time quantitative polymerse chain reaction (PCR) detection. The kit disclosed by the invention evaluates the metabolic capability of individual folate by simultaneously detecting single nucleotide polymorphism site genotype of the 5,10-methylene tetrahydrofolate reductase gene and the methionine synthase reductase gene of folate metabolism.
Owner:浙江爱易生物医学科技有限公司
Folate metabolism related gene MTHFR heritable variation detection kit and application thereof
InactiveCN105803107AEasy to readReduce manufacturing costMicrobiological testing/measurementDNA/RNA fragmentationForward primerFolate Metabolism
The invention discloses a folate metabolism related gene MTHFR heritable variation detection kit.The kit is used for identifying rs1801133 genotypes and contains that 1, forward and reverse primers: the forward primer is a nucleotide strand or its complementary strand of a sequence shown by SEQ ID NO.1, and the reverse primer is a nucleotide strand or its complementary strand of a sequence shown by SEQ ID NO.2; 2, a PCR reaction: 2 x HRM PreMix.In addition, the invention further discloses application of the kit.The detection kit adopts polymerase chain reaction, a reaction system contains specific forward and reverse primers, and the genotypes are judged through real-time fluorescence quantification detection.The folate metabolism related gene MTHFR heritable variation detection kit has the advantages of being low in cost, good in specificity, high in sensitivity, simple and quick to operate and accurate in classification, making results easily interpreted and the like, can be used for clinic detection of folate metabolism related gene MTHFR heritable variation and assists a doctor to select an appropriate dose of folic acid.
Owner:SHANGHAI HEDUN BIOTECH CO LTD
Primers and fluorescent probes used for detecting folate metabolism related gene SNP and application
InactiveCN108034710AThe test results are accurate and reliableLow costMicrobiological testing/measurementDNA/RNA fragmentationFolate MetabolismAgricultural science
The invention discloses primers and fluorescent probes used for detecting folate metabolism related gene SNP and an application. The primers include four groups, each group having a corresponding fluorescent probe group in order to perform quick genetic typing to rs1801133, rs1801131, and rs1801394. The primers and fluorescent probes have accurate and reliable detection results and low cost, and have good practicability.
Owner:SHANGHAI PERSONAL BIOTECH
Combined detection method for pregnancy folate metabolism, calcium metabolism and H-type hypertension
PendingCN110257507AImprove throughputImprove featuresMicrobiological testing/measurementDNA/RNA fragmentationCalcium metabolismSequence analysis
The invention relates to a combined detection method for pregnancy folate metabolism, calcium metabolism and H-type hypertension. The method is characterized in that bioinformatics knowledge and related bioinformatics software are utilized to perform homology analysis, peripheral SNP analysis, frequency analysis, base content analysis and SNP peripheral sequence analysis on the sequence information of multiple SNP loci (rs10512366, rs1544410, rs1801131, rs1801133, rs2234693, rs9340799, rs731236, rs7975232 and rs1801394) which can retrieved in an open database, and then a specific multiple PCR primer system and a multiple LDR probe system are designed and optimized. The method has the advantages that fragment length analysis is performed by combining the multiple PCR-LDR technology and the capillary electrophoresis technology of a genetic analyzer to achieve SNP detection typing, and the method is large in flux, high in specificity and flexibility, stable in result, good in repeatability and fast in detection.
Owner:上海联吉医学检验所有限公司
Method for detecting polymorphism of folate metabolism related genes through whole-blood direct nucleic acid amplification
InactiveCN106868180APCR conditions are broadNo pre-experimentMicrobiological testing/measurementDNA preparationFolate MetabolismMthfr c677t
The invention discloses a method for detecting polymorphism of folate metabolism related genes through whole-blood direct nucleic acid amplification. The method comprises the following steps: adopting a whole-blood sample as a starting material, adding primers for amplifying MTHFR C677T and MTHFR A1298C polymorphic region fragments, as well as blood resistance taq polymerase buffer and blood resistance taq polymerase in a nucleic acid amplification reaction reagent, so that a PCR system is constituted for directly conducting a nucleic acid amplification reaction; and a PCR product is directly delivered to a commercial company for being subjected to sequencing, so that workload is reduced. A result can be clearly judged by analyzing a mutation site on a sequencing diagram, with an accuracy rate reaching 100%. The method is simple to operate, low in cost, rapid and accurate, and high in throughput; detection of normal hospitals in second and third-tier cities can be satisfied; and the method is applicable to clinical detection and popularization.
Owner:浙江中迪生物科技有限公司
Method and kit for detecting genetic information of folate metabolism related genes
ActiveCN108642138ASuitable for large-scale productionShorten the timeMicrobiological testing/measurementDNA/RNA fragmentationFolate MetabolismEnzyme digestion
The invention discloses a method and a kit for detecting genetic information of folate metabolism related genes, and relates to three sites, namely an MTHFR gene rs1801133 site, an rs1801131 site andan MTRR gene rs1801394 site. The method and kit provided by the invention, on the basis of the comprehensive use of such technologies as direct PCR, nested PCR, multiple PCR, created restriction sitemethod PCR, PCR-RFLP and the like, can implement PCR amplifications by two turns in a mode of directly using cells without extracting DNA, and the second turn of PCR product of each site can use cheaprestriction endonuclease to conduct enzyme digestion typing; in accordance with actual demands, the three sites can be simultaneously detected within a same tube, or two sites can be simultaneously detected within a same tube, or any one of the sites can be independently detected. All PCR products take enzyme digestion internal control sequences as quality control of enzyme digestion, so that theaccuracy of result judging. The method is simple and convenient in operation of a whole process and is low cost; and accuracy is quite guaranteed.
Owner:GUANGDONG PHARMA UNIV
Gene sequence variances in genes related to folate metabolism having utility in determining the treatment of disease
InactiveUS20100099087A1Safe and effective useEffective treatmentMicrobiological testing/measurementFolate MetabolismPyrimidine transport
The present disclosure describes the use of genetic variance information for folate transport or metabolism genes or pyrimidine transport or metabolism genes in the selection of effective methods of treatment of a disease or condition. The variance information is indicative of the expected response of a patient to a method of treatment. Methods of determining relevant variance information and additional methods of using such variance information are also described.
Owner:STANTON JR VINCENT P
Method for detecting folate metabolism-related gene and kit thereof
The invention discloses a method for detecting folate metabolism-related gene. The method comprises the following steps: subject nucleic acid is obtained, the nucleic acid comprises DNA fragment; at least two SNP bits genotype are detected, MTRR gene c.66A>G; MTHFR gene c.667C>T; and MTHFR gene c.1298A>C; a first primer amplification comprise the DNA fragment of the SNP bit, the amplification product is obtained, a second primer is combined with the amplification product, a base is extended, an extension product is obtained, according to the molecular weight difference of the extension product and the second primer, the base type of the SNP bit is determined. The invention also discloses a kit for detecting folate metabolism capability gene. By using the method and / or the kit, the detecting folate metabolism-related gene can be typed with high flux, low cost, high accuracy and short time consumption, and the typing result can help the subject for supplying folic acid.
Owner:天津华大基因科技有限公司 +1
Primer pair and kit for detecting folate metabolism-related gene polymorphism in hypertensive patients
InactiveCN107586837AEasy to handleHigh sensitivityMicrobiological testing/measurementDNA/RNA fragmentationPositive controlBiotin
The invention relates to a primer pair and a kit for detecting folate metabolism-related gene polymorphism in hypertensive patients, and belongs to the technical field of in vitro nucleic acid detection. The primer pair comprises a forward amplification primer, a reverse amplification primer and a sequencing primer, wherein the 5' end of the reverse amplification primer is labeled with biotin. Thekit comprises a PCR (Polymerase Chain Reaction) solution containing a forward amplification primer and a reverse amplification primer, a sequencing primer, uracil DNA (Deoxyribonucleic Acid) glycosylase, Taq polymerase and a positive control product. The kit provided by the invention has the advantages of accurate detection result, high specificity, short detection period, and simple operation, and the clinical inspection requirements can be effectively met; in addition, the kit also has the advantages of real-time monitoring of reaction progress, and short reaction time, the PCR product canbe used for sequencing on a pyrosequencing instrument after being simply treated, the sensitivity is higher than that of a gold standard method, i.e., a capillary electrophoresis sequencing method, and the kit is more applicable to mutation analysis.
Owner:CHANGSHA 3G BIOTECH
Liquid chromatography tandem mass spectrometry detection kit and detection method for testing folic acid metabolism derivatives of human body
The invention discloses a liquid chromatography tandem mass spectrometry detection kit and a detection method for testing folic acid metabolism derivatives of a human body, and belongs to the technical field of analysis and detection. The liquid chromatography tandem mass spectrometry detection kit for testing the folic acid metabolism derivatives of the human body can be used for detecting folic acid, tetrahydrofolic acid, 5-methyltetrahydrofolic acid, 5-formyltetrahydrofolic acid, 5,10-methylenetetrahydrofolate, S-adenosylmethionine, S-adenosylhomocysteine and homocysteine serum (plasma). The method has the characteristics of simplicity, rapidness, strong specificity, high sensitivity, comprehensive detection indexes and the like; the method can provide a reference basis for knowing the folic acid metabolism condition in clinical application.
Owner:广东南芯医疗科技有限公司 +1
Kit for detecting genotyping of folic acid metabolizing genes
InactiveCN109234374AReduce pollutionImprove detection efficiencyMicrobiological testing/measurementDNA/RNA fragmentationFolate MetabolismEnzyme system
The invention provides a kit for detecting the genotyping of folic acid metabolizing genes, and belongs to the technical field of molecular biology. Specifically, RNase H2 and Taq DNA polymerase biphasic enzyme systems were used to detect the folate metabolism-related gene loci. The kit provided by the invention has the advantages of high sensitivity, fast detection, good stability and closed detection, and reduces the possibility of later pollution.
Owner:WUHAN CMLABS CO LTD
Composition, kit and method for detecting human folate metabolism gene polymorphism
PendingCN111187828AImprove accuracyEasy to operateMicrobiological testing/measurementDNA/RNA fragmentationFolate MetabolismGene
The invention relates to the field of detection of molecular biology, in particular to the field of detection of folate metabolism capability, provides composition for detecting the folate metabolismcapability and also provides an application of the composition for detecting the folate metabolism capability, a kit containing the composition and a method for detecting the folate metabolism capability. With the adoption of the composition and the method, two SNP types can be detected through one channel, multiple detection through one tube is realized, time and cost are saved, and samples for computer detection can be increased.
Owner:SANSURE BIOTECH INC
Gene detection method of amount of individual folic acid replenisher
InactiveCN110241195AMicrobiological testing/measurementDNA/RNA fragmentationFolate MetabolismGene type
The invention provides a gene detection method of amount of an individual folic acid replenisher. Through design of a primer for amplifying rs1801133, rs1801131, rs1805087 and rs1801394 sites, 4 SNP sites tightly relevant with metabolism of folic acid in individuals are amplified, and the gene type of individual amplification fragments is further analyzed. According to the difference of idiotype, the quantity demanded of the folic acid is recommended. The method is simple to operate and high in practicability, and is suitable for promotion and use.
Owner:QINGHAI UNIVERSITY
Compound sulfamonomethoxine daimeton injection and preparing method thereof
InactiveCN101199536AEnhanced inhibitory effectGood curative effectAntibacterial agentsTetracycline active ingredientsFolate MetabolismSulfamonomethoxine
The invention relates to compound sulfamonomethoxine injection and a preparation method of the compound sulfamonomethoxine injection. The injection comprises main drug, antioxidant, solvent for injection and water for injection. The preparation method is that the sulfamonomethoxine and trimethoprim (TMP) are used together, which can doubly block folate metabolism of bacteria; under the bidirectional cooperative effect between cyclooxygenase and ethyl inhibitor, the sulfamonomethoxine and doxycycline cooperate, so the drug absorption is fast and the toxicity is reduced with better efficacy and more safety. With scientific compatibility, lower cost and simple and easy preparation method, the invention is suitable for industrialized mass production.
Owner:浙江道格凯特实业有限公司
Expression vector system comprising two selection markers
Owner:NOVARTIS AG
Ophthalmic gel for konjac glucomannan and preparation method thereof
InactiveCN107184545AEffective treatmentEasy bioavailabilityAntibacterial agentsOrganic active ingredientsSolubilityPurine
The invention relates to an ophthalmic gel for konjac glucomannan and a preparation method thereof. The preparation method of the ophthalmic gel for konjac glucomannan comprises preparing konjac glucomannan purified powder, preparing a substrate, preparing a medicine liquor and preparing the gel. The konjac glucomannan is used as a main material and serves as a carrier, the gel property, water-retaining property, water-solubility and antibacterial property of the gel are utilized, sulfacetamide sodium is assisted, and due to dihydrofolate synthetase for para aminobenzoic acid competition bacteria, the folate metabolism of bacteria is blocked, required purine and nucleic acid cannot be obtained, and the growth and propagation of the bacteria are restricted. The konjac glucomannan is stable in drug carrying ability, and the prepared ophthalmic gel for konjac glucomannan is high in bioavailability, high in mold resistance and good in water solubility. The ophthalmic gel for konjac glucomannan has the advantages of simple materials, convenient material taking, low cost, easy preparation, good water solubility, good biodegradable and bioavailability, nontoxicity, innocuity and the like, and the novel efficient ophthalmic gel new product is increased for the field of medical treatment and health.
Owner:FUJIAN AGRI & FORESTRY UNIV
Method for detecting polymorphism of folate metabolism-related genes
ActiveCN110157796AImprove detection efficiencyPolymorphic period is shortMicrobiological testing/measurementAgainst vector-borne diseasesDigestion TreatmentGenomic DNA
The invention provides a method for detecting polymorphism of folate metabolism-related genes. The method comprises the steps that specific amplification primers and single base extension primers of the C677T site and A1298C site of an MTHFR gene and the A66G site of an MTRR gene are synthesized in advance; to-be-detected sample genomic DNA is extracted from a to-be-detected sample to serve as a DNA template; a polymerase chain reaction PCR amplification system containing the specific amplification primers and the DNA template is prepared; a PCR apparatus is used for amplifying the PCR amplification system; digestion treatment is performed in sequence; a single base extension reaction system containing the single base extension primers is added into a reaction system for extension reactions in sequence, desalted purification treatment is performed in sequence, and a purified product is obtained; a detection instrument containing a mass spectrometer is used for detecting the purified product to determine the gene polymorphism of the C677T site and A1298C site of the MTHFR gene and the A66G site of the MTRR gene of the to-be-detected sample. The method for detecting the polymorphismof the folate metabolism-related genes can improve the detection efficiency of folate metabolism capacity in the body of a patient.
Owner:BEIJING HARMONY HEALTH MEDICAL DIAGNOSTICS CO LTD
Pharmaceutical composition for preventing and treating black gill disease of aquatic animals
InactiveCN102138994AInhibition of growth and reproductionRegulate metabolic functionAntibacterial agentsAntimycoticsDiseaseSide effect
The invention relates to a pharmaceutical composition for preventing and treating the black gill disease of aquatic animals. The pharmaceutical composition is prepared from the following components in parts by weight: 50 parts of cortex dictamni, 75 parts of common cnidium fruit, 50 parts of golden larch bark, 40 parts of artemisia leaves, 45 parts of sophora flavescens, 45 parts of fineleaf schizonepeta herb, 25 parts of ephedra, 30 parts of common threewingnut root and 25 parts of gypsum rubrum. In the invention, the synergy of all the components is exerted through selecting and reasonably proportioning the pharmaceutical compositions, bacterial growth and reproduction are inhibited through interfering the protein synthesis and the folate metabolism of sensitive pathogenic microorganisms, and the pharmaceutical composition can be used for preventing and treating symptoms of aquatic animals caused by various bacteria and fungi, such as black gills, yellow gills, gill rotting, brown spots, tail rotting, body rotting, and the like. The invention is applied to remarkably improving the water quality, obviously adding the feed quantity and obviously reducing unintelligible death; and the special natural biological active substances in the pharmaceutical composition can control the metabolism of organisms and help the aquatic animals recover. Meanwhile, the pharmaceutical composition provided by the invention is a pure Chinese medicine preparation without any toxic and side effects and ca not form pharmaceutical residue in the aquatic animals.
Owner:仙桃市魏氏生物工程有限责任公司
Expression vector system comprising two selection markers
ActiveUS20110306092A1High yieldIncrease selective pressureAnimal cellsBacteriaFolate MetabolismInsertion site
The invention pertains to an expression vector or a combination of at least two expression vectors comprising at least(a) a polynucleotide encoding a product of interest or an insertion site for incorporating a polynucleotide encoding a product of interest;(b) a polynucleotide encoding a first selectable marker (sm I);(c) a polynucleotide encoding a second selectable marker (sm II), which is different from the first selectable marker (sm I),wherein the activity of the selectable marker (sm I) or (sm II) is at least partially influenced by the activity of the other selectable marker and wherein the selectable markers (sm I) and (sm II) are involved in the folate metabolism. Also provided are suitable host cells, selection methods and methods for producing polypeptides with high yield.
Owner:NOVARTIS AG
Detection kit and detection method for folate metabolism related molecular marker gene mutation
PendingCN112522375AEasy to operateThe detection process is fastMicrobiological testing/measurementDNA/RNA fragmentationGenes mutationFolate Metabolism
The invention discloses crRNA, a kit and a method for detecting folate metabolism related molecular marker gene mutation. The crRNA comprises crRNA of a mutation site of MTHFR-667 with a sequence as shown in any one of SEQ ID NO.11-12; crRNA of a mutation site of MTHFR-1298 with a sequence as shown in any one of SEQ ID NO.13-14; and crRNA of a mutation site of MTRR-66 gene with a sequence as shownin any one of SEQ ID NO. 15-16. By screening crRNA and combining with a CRISPR-Cpf1 system, whether folate metabolism related molecular marker gene mutation exists in a to-be-detected sample or not can be detected in a short time, the kit and the detection method are easy to operate, high in detection speed and low in cost, repeated detection can be achieved, and meanwhile detection sensitivity and detection specificity are remarkably improved.
Owner:江苏博嘉生物医学科技有限公司
Method and kit for detecting genes related to folic acid metabolism
The invention discloses a method for detecting folate metabolism-related gene. The method comprises the following steps: subject nucleic acid is obtained, the nucleic acid comprises DNA fragment; at least two SNP bits genotype are detected, MTRR gene c.66A>G; MTHFR gene c.667C>T; and MTHFR gene c.1298A>C; a first primer amplification comprise the DNA fragment of the SNP bit, the amplification product is obtained, a second primer is combined with the amplification product, a base is extended, an extension product is obtained, according to the molecular weight difference of the extension product and the second primer, the base type of the SNP bit is determined. The invention also discloses a kit for detecting folate metabolism capability gene. By using the method and / or the kit, the detecting folate metabolism-related gene can be typed with high flux, low cost, high accuracy and short time consumption, and the typing result can help the subject for supplying folic acid.
Owner:天津华大基因科技有限公司 +1
Method for improving content of 5-methyltetrahydrofolate by gene knockout
ActiveCN111041045AIncrease contentAlleviate hidden hungerTransferasesVector-based foreign material introductionBiotechnologyNutrition
The invention provides a method for increasing the content of 5-methyltetrahydrofolate through gene knockout. Corn ZmGFT1 is subjected to gene knockout through a CRISPR / Cas9 technology, and it is found that the content of 5-methyltetrahydrofolate in the corn after gene knockout is remarkably increased. According to the invention, the design of the CRISPR / Cas9 target site of corn ZmGFT1 and the function of the gene can significantly influence the folic acid content; the gene provides reference for CRISPR research of the gene in corn and other crops, provides a theoretical basis for folic acid metabolism of corn and other crops, and relieves the invisible hunger problem of human beings in the aspect of folic acid nutrition by increasing the folic acid content of transgenic plants.
Owner:THE INST OF BIOTECHNOLOGY OF THE CHINESE ACAD OF AGRI SCI
Primer probe combination for detecting polymorphism of folate metabolism related genes and application of primer probe combination
PendingCN114350791ASimple and fast operationEasy to operateMicrobiological testing/measurementDNA/RNA fragmentationFolate MetabolismNucleotide
The invention provides a primer probe combination for detecting the polymorphism of a folate metabolism related gene and application of the primer probe combination. The primer probe combination for detecting the polymorphism of the folate metabolism related gene specifically amplifies and detects the polymorphism conditions of an MTHFR (Methylene Tetrahydrofolate Reductase) gene C677T, an MTHFR gene A1298C and an MTRR (Methylene Tetrahydrofolate Reductase) gene A66G; an upstream primer of a specific primer pair for amplifying the MTHFR gene C677T comprises a nucleotide sequence as shown in SEQ ID No. 1, a downstream primer of the specific primer pair for amplifying the MTHFR gene C677T comprises a nucleotide sequence as shown in SEQ ID No. 2, and a probe for detecting the MTHFR gene C677T comprises a nucleotide sequence as shown in SEQ ID No. 3. The invention also provides a kit for detecting the polymorphism of the folate metabolism related gene. The primer probe combination is high in sensitivity, good in specificity, high in detection efficiency, easy to operate and wide in application prospect.
Owner:SHANGHAI BIOTECAN PHARMA +3
Method for detecting mutation site of folate metabolism related gene
InactiveCN109988831AMicrobiological testing/measurementDNA/RNA fragmentationMass spectrometricBiomedicine
The invention belongs to the field of biomedicine, solves the problem that one-way single base extension accurately determines a mutation site difficultly, and discloses a method for detecting a mutation site of a folate metabolism related gene. A target fragment is obtained through a single-tube multiplex PCR amplification reaction of a designed target site specific primer, then the target fragment is bidirectionally extended by using a single base extension technology to obtain a to-be-detected gene site, and finally a target DNA sequence is accurately determined through the nucleic acid mass spectrum analysis of the to-be-detected gene site, wherein the single base extension technology involves that a single base extension primer is adopted for bidirectionally extending the target fragment. The invention aims to perform mutation detection on the folate metabolism related gene by the bidirectional single base extension technology, thereby providing safety guidance for individual clinical medication.
Owner:杭州云鼎基因生物科技有限公司
A method for increasing the content of 5-methyltetrahydrofolate by gene knockout
ActiveCN111041045BIncrease contentAlleviate hidden hungerTransferasesVector-based foreign material introductionBiotechnologyFolate Metabolism
The invention provides a method for increasing the content of 5-methyltetrahydrofolate through gene knockout. Maize by CRISPR / Cas9 technology ZmGFT1 The gene knockout was implemented, and it was found that the content of 5-methyltetrahydrofolate in maize was significantly increased after the gene knockout. Corn in the present invention ZmGFT1 The design of the CRISPR / Cas9 target site and the discovery that the function of the gene can significantly affect the folic acid content not only provide a reference for the CRISPR research of this gene in maize and other crops, but also provide a theory for the folic acid metabolism of maize and other crops The foundation, by increasing the folic acid content of transgenic plants, alleviates the hidden hunger problem of human beings in folic acid nutrition.
Owner:THE INST OF BIOTECHNOLOGY OF THE CHINESE ACAD OF AGRI SCI
Who we serve
- R&D Engineer
- R&D Manager
- IP Professional
Why Patsnap Eureka
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com