33 results about "Folate Metabolism" patented technology

The present disclosure describes the use of genetic variance information for folate transport or metabolism genes or pyrimidine transport or metabolism genes in the selection of effective methods of treatment of a disease or condition. The variance information is indicative of the expected response of a patient to a method of treatment. Methods of determining relevant variance information and additional methods of using such variance information are also described.

The invention pertains to an expression vector or a combination of at least two expression vectors comprising at least(a) a polynucleotide encoding a product of interest or an insertion site for incorporating a polynucleotide encoding a product of interest;(b) a polynucleotide encoding a first selectable marker (sm I);(c) a polynucleotide encoding a second selectable marker (sm II), which is different from the first selectable marker (sm I),wherein the activity of the selectable marker (sm I) or (sm II) is at least partially influenced by the activity of the other selectable marker and wherein the selectable markers (sm I) and (sm II) are involved in the folate metabolism. Also provided are suitable host cells, selection methods and methods for producing polypeptides with high yield.

The invention discloses a method for detecting folate metabolism-related gene. The method comprises the following steps: subject nucleic acid is obtained, the nucleic acid comprises DNA fragment; at least two SNP bits genotype are detected, MTRR gene c.66A>G; MTHFR gene c.667C>T; and MTHFR gene c.1298A>C; a first primer amplification comprise the DNA fragment of the SNP bit, the amplification product is obtained, a second primer is combined with the amplification product, a base is extended, an extension product is obtained, according to the molecular weight difference of the extension product and the second primer, the base type of the SNP bit is determined. The invention also discloses a kit for detecting folate metabolism capability gene. By using the method and / or the kit, the detecting folate metabolism-related gene can be typed with high flux, low cost, high accuracy and short time consumption, and the typing result can help the subject for supplying folic acid.

The invention relates to a pharmaceutical composition for preventing and treating the black gill disease of aquatic animals. The pharmaceutical composition is prepared from the following components in parts by weight: 50 parts of cortex dictamni, 75 parts of common cnidium fruit, 50 parts of golden larch bark, 40 parts of artemisia leaves, 45 parts of sophora flavescens, 45 parts of fineleaf schizonepeta herb, 25 parts of ephedra, 30 parts of common threewingnut root and 25 parts of gypsum rubrum. In the invention, the synergy of all the components is exerted through selecting and reasonably proportioning the pharmaceutical compositions, bacterial growth and reproduction are inhibited through interfering the protein synthesis and the folate metabolism of sensitive pathogenic microorganisms, and the pharmaceutical composition can be used for preventing and treating symptoms of aquatic animals caused by various bacteria and fungi, such as black gills, yellow gills, gill rotting, brown spots, tail rotting, body rotting, and the like. The invention is applied to remarkably improving the water quality, obviously adding the feed quantity and obviously reducing unintelligible death; and the special natural biological active substances in the pharmaceutical composition can control the metabolism of organisms and help the aquatic animals recover. Meanwhile, the pharmaceutical composition provided by the invention is a pure Chinese medicine preparation without any toxic and side effects and ca not form pharmaceutical residue in the aquatic animals.