33 results about "Folate Metabolism" patented technology

The invention provides a kit for genotyping folate metabolism gene. The kit includes a PCR reaction liquid and a DNA hybrid membrane strip, the DNA hybrid film strip comprises a matrix vector and probes, four probes for MTHFR genes, two probes for MTRR genes and two probes designed for human genome are sequentially fixed on the matrix vector, each of the probes is an oligonucleotide sequence hybridized with the SNP locus of each of the corresponding gene, and the nucleotide sequences of the probes are represented by SEQ ID NO:1-8. The kit provided by the invention has the advantages of high sensitivity, rapid detection, good stability, realization of high or low flux detection, high flexibility, strong maneuverability for small clinical samples in some hospitals, less equipment investment and low cost.

The present disclosure describes the use of genetic variance information for folate transport or metabolism genes or pyrimidine transport or metabolism genes in the selection of effective methods of treatment of a disease or condition. The variance information is indicative of the expected response of a patient to a method of treatment. Methods of determining relevant variance information and additional methods of using such variance information are also described.

The invention discloses a detection method of a folate metabolism related gene, which includes steps of extracting DNA from oral mucosa cell of a detector; amplifying MTHFR gene C677T by SEQ NO 1 and SEQ NO 2; amplifying MTHFR gene A1298C by SEQ NO 3 and SEQ NO 4; amplifying MTHFR gene A66G by SEQ NO 5 and SEQ NO 6; extracting DNA from oral mucosa cell as a template to perform PCR reaction; performing sepharose gel electrophoresis of 1% of ordinary PCR amplified product on three pairs of primers; recycling a strip of a kit recycling item from the 1% of agarose gel electrophoretic band by sepharose gel; performing sequence testing reaction; purifying the sequence testing reaction product and denaturing; testing sequence by an upper 3730 sequence tester; analyzing three SNP gene models of the sequence testing result by Chromas software. The method has the advantages of high detection accuracy, and convenience of use.

The invention discloses a kit for detecting an individual folate metabolism disorder. The kit comprises a specific primer pair and SYBRGreenI fluorochrome for simultaneously detecting rs1801133# SNP site on a 5,10-methylene tetrahydrofolate reductase gene, rs1801131# SNP on a 5,10-methylene tetrahydrofolate reductase gene and rs1801394# SNP site on a methionine synthase reductase gene, a conventional component for real-time quantitative polymerse chain reaction (PCR) detection. The kit disclosed by the invention evaluates the metabolic capability of individual folate by simultaneously detecting single nucleotide polymorphism site genotype of the 5,10-methylene tetrahydrofolate reductase gene and the methionine synthase reductase gene of folate metabolism.

The invention discloses a folate metabolism related gene MTHFR heritable variation detection kit.The kit is used for identifying rs1801133 genotypes and contains that 1, forward and reverse primers: the forward primer is a nucleotide strand or its complementary strand of a sequence shown by SEQ ID NO.1, and the reverse primer is a nucleotide strand or its complementary strand of a sequence shown by SEQ ID NO.2; 2, a PCR reaction: 2 x HRM PreMix.In addition, the invention further discloses application of the kit.The detection kit adopts polymerase chain reaction, a reaction system contains specific forward and reverse primers, and the genotypes are judged through real-time fluorescence quantification detection.The folate metabolism related gene MTHFR heritable variation detection kit has the advantages of being low in cost, good in specificity, high in sensitivity, simple and quick to operate and accurate in classification, making results easily interpreted and the like, can be used for clinic detection of folate metabolism related gene MTHFR heritable variation and assists a doctor to select an appropriate dose of folic acid.

The invention discloses primers and fluorescent probes used for detecting folate metabolism related gene SNP and an application. The primers include four groups, each group having a corresponding fluorescent probe group in order to perform quick genetic typing to rs1801133, rs1801131, and rs1801394. The primers and fluorescent probes have accurate and reliable detection results and low cost, and have good practicability.

The invention relates to a combined detection method for pregnancy folate metabolism, calcium metabolism and H-type hypertension. The method is characterized in that bioinformatics knowledge and related bioinformatics software are utilized to perform homology analysis, peripheral SNP analysis, frequency analysis, base content analysis and SNP peripheral sequence analysis on the sequence information of multiple SNP loci (rs10512366, rs1544410, rs1801131, rs1801133, rs2234693, rs9340799, rs731236, rs7975232 and rs1801394) which can retrieved in an open database, and then a specific multiple PCR primer system and a multiple LDR probe system are designed and optimized. The method has the advantages that fragment length analysis is performed by combining the multiple PCR-LDR technology and the capillary electrophoresis technology of a genetic analyzer to achieve SNP detection typing, and the method is large in flux, high in specificity and flexibility, stable in result, good in repeatability and fast in detection.

The invention discloses a method for detecting polymorphism of folate metabolism related genes through whole-blood direct nucleic acid amplification. The method comprises the following steps: adopting a whole-blood sample as a starting material, adding primers for amplifying MTHFR C677T and MTHFR A1298C polymorphic region fragments, as well as blood resistance taq polymerase buffer and blood resistance taq polymerase in a nucleic acid amplification reaction reagent, so that a PCR system is constituted for directly conducting a nucleic acid amplification reaction; and a PCR product is directly delivered to a commercial company for being subjected to sequencing, so that workload is reduced. A result can be clearly judged by analyzing a mutation site on a sequencing diagram, with an accuracy rate reaching 100%. The method is simple to operate, low in cost, rapid and accurate, and high in throughput; detection of normal hospitals in second and third-tier cities can be satisfied; and the method is applicable to clinical detection and popularization.

The invention discloses a method for detecting folate metabolism-related gene. The method comprises the following steps: subject nucleic acid is obtained, the nucleic acid comprises DNA fragment; at least two SNP bits genotype are detected, MTRR gene c.66A>G; MTHFR gene c.667C>T; and MTHFR gene c.1298A>C; a first primer amplification comprise the DNA fragment of the SNP bit, the amplification product is obtained, a second primer is combined with the amplification product, a base is extended, an extension product is obtained, according to the molecular weight difference of the extension product and the second primer, the base type of the SNP bit is determined. The invention also discloses a kit for detecting folate metabolism capability gene. By using the method and/or the kit, the detecting folate metabolism-related gene can be typed with high flux, low cost, high accuracy and short time consumption, and the typing result can help the subject for supplying folic acid.

The invention relates to the field of detection of molecular biology, in particular to the field of detection of folate metabolism capability, provides composition for detecting the folate metabolismcapability and also provides an application of the composition for detecting the folate metabolism capability, a kit containing the composition and a method for detecting the folate metabolism capability. With the adoption of the composition and the method, two SNP types can be detected through one channel, multiple detection through one tube is realized, time and cost are saved, and samples for computer detection can be increased.

The invention relates to an ophthalmic gel for konjac glucomannan and a preparation method thereof. The preparation method of the ophthalmic gel for konjac glucomannan comprises preparing konjac glucomannan purified powder, preparing a substrate, preparing a medicine liquor and preparing the gel. The konjac glucomannan is used as a main material and serves as a carrier, the gel property, water-retaining property, water-solubility and antibacterial property of the gel are utilized, sulfacetamide sodium is assisted, and due to dihydrofolate synthetase for para aminobenzoic acid competition bacteria, the folate metabolism of bacteria is blocked, required purine and nucleic acid cannot be obtained, and the growth and propagation of the bacteria are restricted. The konjac glucomannan is stable in drug carrying ability, and the prepared ophthalmic gel for konjac glucomannan is high in bioavailability, high in mold resistance and good in water solubility. The ophthalmic gel for konjac glucomannan has the advantages of simple materials, convenient material taking, low cost, easy preparation, good water solubility, good biodegradable and bioavailability, nontoxicity, innocuity and the like, and the novel efficient ophthalmic gel new product is increased for the field of medical treatment and health.

The invention relates to a pharmaceutical composition for preventing and treating the black gill disease of aquatic animals. The pharmaceutical composition is prepared from the following components in parts by weight: 50 parts of cortex dictamni, 75 parts of common cnidium fruit, 50 parts of golden larch bark, 40 parts of artemisia leaves, 45 parts of sophora flavescens, 45 parts of fineleaf schizonepeta herb, 25 parts of ephedra, 30 parts of common threewingnut root and 25 parts of gypsum rubrum. In the invention, the synergy of all the components is exerted through selecting and reasonably proportioning the pharmaceutical compositions, bacterial growth and reproduction are inhibited through interfering the protein synthesis and the folate metabolism of sensitive pathogenic microorganisms, and the pharmaceutical composition can be used for preventing and treating symptoms of aquatic animals caused by various bacteria and fungi, such as black gills, yellow gills, gill rotting, brown spots, tail rotting, body rotting, and the like. The invention is applied to remarkably improving the water quality, obviously adding the feed quantity and obviously reducing unintelligible death; and the special natural biological active substances in the pharmaceutical composition can control the metabolism of organisms and help the aquatic animals recover. Meanwhile, the pharmaceutical composition provided by the invention is a pure Chinese medicine preparation without any toxic and side effects and ca not form pharmaceutical residue in the aquatic animals.

The invention pertains to an expression vector or a combination of at least two expression vectors comprising at least

• • (a) a polynucleotide encoding a product of interest or an insertion site for incorporating a polynucleotide encoding a product of interest;
  • (b) a polynucleotide encoding a first selectable marker (sm I);
  • (c) a polynucleotide encoding a second selectable marker (sm II), which is different from the first selectable marker (sm I),

wherein the activity of the selectable marker (sm I) or (sm II) is at least partially influenced by the activity of the other selectable marker and wherein the selectable markers (sm I) and (sm II) are involved in the folate metabolism. Also provided are suitable host cells, selection methods and methods for producing polypeptides with high yield.

The invention provides a method for increasing the content of 5-methyltetrahydrofolate through gene knockout. Corn ZmGFT1 is subjected to gene knockout through a CRISPR/Cas9 technology, and it is found that the content of 5-methyltetrahydrofolate in the corn after gene knockout is remarkably increased. According to the invention, the design of the CRISPR/Cas9 target site of corn ZmGFT1 and the function of the gene can significantly influence the folic acid content; the gene provides reference for CRISPR research of the gene in corn and other crops, provides a theoretical basis for folic acid metabolism of corn and other crops, and relieves the invisible hunger problem of human beings in the aspect of folic acid nutrition by increasing the folic acid content of transgenic plants.

The invention provides a primer probe combination for detecting the polymorphism of a folate metabolism related gene and application of the primer probe combination. The primer probe combination for detecting the polymorphism of the folate metabolism related gene specifically amplifies and detects the polymorphism conditions of an MTHFR (Methylene Tetrahydrofolate Reductase) gene C677T, an MTHFR gene A1298C and an MTRR (Methylene Tetrahydrofolate Reductase) gene A66G; an upstream primer of a specific primer pair for amplifying the MTHFR gene C677T comprises a nucleotide sequence as shown in SEQ ID No. 1, a downstream primer of the specific primer pair for amplifying the MTHFR gene C677T comprises a nucleotide sequence as shown in SEQ ID No. 2, and a probe for detecting the MTHFR gene C677T comprises a nucleotide sequence as shown in SEQ ID No. 3. The invention also provides a kit for detecting the polymorphism of the folate metabolism related gene. The primer probe combination is high in sensitivity, good in specificity, high in detection efficiency, easy to operate and wide in application prospect.