Primers and fluorescent probes used for detecting folate metabolism related gene SNP and application

A fluorescent probe and folic acid metabolism technology, applied in the biological field, can solve the problems of insufficiency, cross-contamination of PCR products, and low reliability, and achieve the effects of low cost, accurate and reliable detection results, and good practicability.

Inactive Publication Date: 2018-05-15
SHANGHAI PERSONAL BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this method is complicated to operate, and when the sample size is large, it is easy to cause cross-contamination of PCR products, and it is prone to insufficient or excessive digestion, resulting in false negative or false positive results, and low reliability.
Although the specificity of the multiplex PCR method has been improved, the principle of this method is still based on the principle of ordinary PCR, and factors such as primer specificity and low-fidelity Taq enzymes can affect the results
Although the DNA sequencing method is c

Method used

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  • Primers and fluorescent probes used for detecting folate metabolism related gene SNP and application
  • Primers and fluorescent probes used for detecting folate metabolism related gene SNP and application
  • Primers and fluorescent probes used for detecting folate metabolism related gene SNP and application

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Embodiment

[0075] 1. Prepare the following primers and probes:

[0076] (1) Primers and fluorescent probes targeting the rs1801133[MTHFR(C677T)] site of methylenetetrahydrofolate reductase gene:

[0077] 677-F: CTGACCTGAAGCACTTGAAGGAGAA

[0078] 677-R: GGGACGATGGGGCAAGTGA

[0079] W677:FAM-ATGAAATCGGCTCCCGC-MGB

[0080] M677:VIC-ATGAAATCGACTCCCGC-MGB

[0081] (2) Primers and fluorescent probes targeting the rs1801131[MTHFR(A1298C)] site of methylenetetrahydrofolate reductase gene:

[0082] 1298-F: TGAAGAGCAAGTCCCCCCAAGG

[0083] 1298-R:CCACTCCAGCATCACTCACTTTG

[0084] W1298: FAM-CAAAGACACTTTCTTCAC-MGB

[0085] M1298: VIC-CAAAGACACTTGCTTCAC-MGB

[0086] (3) Primers and fluorescent probes targeting the site of methionine synthase reductase rs1801394[MTRR(A66G)]:

[0087] 66-F: TTGAAGTGATGAGGAGGTTTCTGTT

[0088] 66-R: GATCTGCAGAAAATCCATGTACCA

[0089] W66: FAM-CTCACATATTTCTTC-MGB

[0090] M66: VIC-CTCACACATTTCTT-MGB

[0091] The above three sets of primers were prepared into a m...

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Abstract

The invention discloses primers and fluorescent probes used for detecting folate metabolism related gene SNP and an application. The primers include four groups, each group having a corresponding fluorescent probe group in order to perform quick genetic typing to rs1801133, rs1801131, and rs1801394. The primers and fluorescent probes have accurate and reliable detection results and low cost, and have good practicability.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a primer, a fluorescent probe and an application for detecting the SNP of a gene related to folic acid metabolism. Background technique [0002] Folic acid is one of the vitamin B complexes, which is equivalent to pteroylglutamic acid (PGA). It was extracted and purified by Mitchell (H.K. Mitchell, 1941) from spinach leaves, so it is named folic acid. Folic acid is an important nutrient needed by the body, and it is rich in green leafy vegetables, fruits and animal liver. Folic acid works in the human body in the form of tetrahydrofolate. Tetrahydrofolate is a carrier of one-carbon units, which can carry one-carbon units such as methyl, methylene, and formaldehyde, and participate in the synthesis of amino acids, purines, and pyrimidines in the body. Therefore, folic acid plays an important role in cell division and proliferation, tissue repair and body development. [0003] etc. a...

Claims

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Application Information

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IPC IPC(8): C12Q1/6883C12Q1/6858C12N15/11
CPCC12Q1/6858C12Q1/6883C12Q2600/156C12Q2531/113C12Q2561/101
Inventor 汪元涛朱月艳孙子奎
Owner SHANGHAI PERSONAL BIOTECH
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