310 results about "Metabolism" patented technology

The invention discloses a method for in vitro slow release performance evaluation of a slow and controlled release preparation based on the overflow principle. A container with an upper outlet and a lower outlet is adopted to serve as a release tank, a to-be-detected medicine and a dissolution medium are placed in the release tank, a stirring device is adopted in the release tank to perform stirring, and the dissolution medium is added from the lower outlet of the release tank through a peristaltic pump; the dissolution medium dissolved with the medicine is taken away through the overflow from the upper outlet so as to offset medicine absorption or metabolism, and the slow and controlled release speed of the medicine, the total medicine overflow quantity and the total release amount are calculated by measuring the concentration of the medicine contained in the dissolution medium flowing out, the volume of the release tank and the overflow quantity. According to the method, the releasing degree of the slow and controlled release medicine can be evaluated accurately, the effective medicine release time of the slow and controlled release preparation is evaluated, and slow release performances of the slow and controlled release preparation are evaluated comprehensively.

The application discloses a kind of exogenous bacteriophage inoculation methods for strengthening soil microbial arsenic methylation capacity, comprising the following steps: S1. preparation bacteriophage crude extract;S2. preparation concentrated solution;S3. preparation bacteriophage extract;S4. preparation inoculation soil sample;S5. in the 2, 4, 6th day of culture, bacteriophage extract is added to inoculation soil;S6. after three times inoculation, continue to flood culture 9 days.The application proposes a kind of inoculation method for retaining virulent bacteriophage, not retaining original host bacteria, inoculating after flooding two days, and inoculating three times, so that the proportion of lysogenization of bacteriophage in soil after inoculation is increased by 156%~347%, so that arsenic metabolism genes carried by bacteriophage genome can function, the arsenic methylation capacity of soil microorganism is strengthened, and the arsenic methylation capacity is increased by more than 300%, to efficiently methylate arsenic in soil.

This invention relates to the fields of microbial genetic engineering and fermentation biomanufacturing technology, specifically disclosing a recombinant Corynebacterium glutamicum with high 5-aminolevulinic acid (ALA) production and its applications. Addressing the problems of existing strains such as crude enzyme activity regulation, inhibited cell growth, the need for external inhibitors, and low yield, this invention uses Corynebacterium glutamicum as a host and removes the gdhA gene without scarring to reduce precursor competitive metabolism. Site-directed mutations of the endogenous hemB gene (S288A, D128E, R231K) are performed to moderately reduce dehydratase activity. A high-expression plasmid pXMJ19-hemA containing the hemA gene from Rhodopsinus capsulatum is constructed and introduced into the modified strain to obtain a recombinant strain with optimized triple metabolism. This strain reduces product degradation without the need for external dehydratase inhibitors, does not affect cell growth, and achieves a yield up to 4.30 times higher than the control after 42 hours of fermentation, with ALA accumulation exceeding 8.9 g / L. The strain exhibits stable characteristics, the process is simple, and it has good industrial application value.

This invention discloses the application of green tea acidic polysaccharide GTPS in improving metabolic syndrome, aiming to provide a new use for green tea acidic polysaccharide GTPS. The extraction process involves first grinding green tea into powder, boiling, filtering, deproteinizing using the Seval method, concentrating, adding ethanol, allowing it to stand overnight, centrifuging to collect the precipitate, dissolving it, removing the ethanol, and freeze-drying to obtain GTPSs. The GTPSs are then passed through a DEAE-agarose FF column, eluted with distilled water and 0.2, 0.5, and 1.0 mol / L sodium chloride, and the fractions are collected. The fraction eluted with 0.2 mol / L sodium chloride is dialyzed to desalt, yielding green tea polysaccharide GTPS. Applying this to the improvement of metabolic syndrome provides a solid foundation for revealing the key active components in green tea that promote metabolism and paves the way for the future utilization of tea polysaccharides in the pharmaceutical and food industries, offering a promising solution for the integration of tea resources and the application of natural products.

This invention belongs to the field of microbial technology, specifically relating to a compound microbial fertilizer that synergistically regulates rhizosphere flora and nitrogen metabolism genes and its application. The microbial agent includes: Bacillus belye (… Bacillus velezensis ), Bacillus subtilis subsp. subtilis ( Bacillus subtilis subsp. subtilis ) and Linyi Streptomyces ( Streptomyces linyiensis Two or three of the following. The microbial agent provided by this invention has significant growth-promoting and nitrogen-fixing effects when applied to crops. It can maintain or even improve soil fertility and crop yield under the condition of reducing nitrogen fertilizer application by 30%, providing a green and efficient new solution for the implementation of "nitrogen reduction and efficiency enhancement".

Bioreactors and associated methods are provided herein including bioreactors capable of treating water contaminated with 1,4-dioxane. In certain embodiments, a bioreactor is disclosed and may include an adsorbent layer with a biofilm capable of metabolizing 1,4-dioxane and a screen disposed downstream of the adsorbent layer configured to retain detached biofilm.

This invention provides the application of a Qi-tonifying and blood-nourishing composition in the preparation of a drug for premenstrual syndrome (PMS), relating to the field of pharmaceutical technology. The application of this Qi-tonifying and blood-nourishing composition in the preparation of a drug for treating a specific phenotype of PMS is characterized by an abnormally low abundance of *Isobacterium* spp. and / or a deficiency in endogenous docosahexaenoic acid (DHA) metabolism. The active ingredients of the Qi-tonifying and blood-nourishing composition include pig blood extract, astragalus, and jujube. This invention precisely targets individuals with a specific phenotype of PMS with a low abundance of *Isobacterium* spp. or DHA deficiency, addressing the underlying mechanism of PMS. The application of a composition containing pig blood extract, astragalus, and jujube can target and correct the aforementioned microecological and metabolic imbalances, achieving targeted and precise treatment and significantly improving the specificity of medication.

The present application provides a kind of metabolism steady state regulation type full nutrition weight loss meal replacement powder, by 82~90 weight parts of endocrine regulation component, 4~8 weight parts of metabolic activation component, 3~5 weight parts of nutrient component and 2~6 weight parts of flavor component, also provides the preparation method of metabolism steady state regulation type full nutrition weight loss meal replacement powder.The present application will realize synchronous intervention to three major core problems of energy metabolism, endocrine coordination, tissue function, reconstructs the metabolic homeostasis of organism, realizes the effective management of body weight.

The application discloses a preparation method of yellow rice wine, the yellow rice wine and application. The preparation method of the yellow rice wine comprises the following steps: gelatinizing starch raw materials to prepare gelatinized starch; uniformly mixing the gelatinized starch, yellow rice wine starter, acidic protease, saccharomyces cerevisiae, yellow rice wine and water to prepare fermentation material, and performing fermentation; adding lactic acid bacteria at 72h-74h of fermentation, and continuing fermentation; and performing solid-liquid separation after the fermentation is completed, collecting clear liquid, decocting wine, aging, and preparing the yellow rice wine. The preparation method of the yellow rice wine breaks through the technical bottleneck that the metabolic time sequence of strains is uncontrollable in traditional mixed fermentation, optimizes the inoculation time of lactic acid bacteria, guarantees the acid production capacity of lactic acid bacteria under the condition of controlling alcohol metabolism, and obtains the yellow rice wine with the characteristics of high sugar, high acid and high ester, which can be used for seasoning.

The present application relates to sewage treatment technical field, specifically to a kind of chemical wastewater desalination integrated processing method.The present application overcomes the problem of low resource utilization efficiency of chemical wastewater desalination in prior art;The present application first synthesizes three-dimensional catalytic aerogel microspheres, which are loaded in a fixed bed to target adsorption and catalytic chain scission of macromolecular polymers in high-salinity wastewater with persulfate;Subsequently, a salt-tolerant membrane bioreactor is introduced to form small-molecule organic compounds through deep metabolism;The effluent is sequentially subjected to target complexation and precipitation of heavy metals using modified chitosan and amphoteric polyacrylamide, pre-implantation of polyepoxysuccinic acid scale inhibitor, and finally subjected to nanofiltration and reverse osmosis systems, freeze crystallization and MVR evaporation to extract high-purity sodium sulfate and sodium chloride.The present application effectively solves the problem of membrane fouling caused by heavy metal leakage and organic matter residue in traditional process, and realizes the deep purification of chemical wastewater and the high-value resource recovery of salt.

This invention discloses a culture medium for manganese-oxidizing bacteria suitable for low-carbon source environments in water supply networks and its application. The culture medium is prepared by mixing ferric ammonium citrate, manganese sulfate, ammonium sulfate, sodium chloride, magnesium sulfate, dipotassium hydrogen phosphate, and sodium nitrate, followed by high-temperature and high-pressure sterilization to obtain a culture solution. Manganese sulfate is added to the culture solution to obtain the manganese-oxidizing bacteria culture medium. Manganese-oxidizing bacteria are screened using this culture medium. Cast iron pipe scale is subcultured in the medium to enrich the manganese-oxidizing bacteria culture solution. The enriched manganese-oxidizing bacteria culture solution is diluted and spread onto agar solid medium, and incubated at a constant temperature to obtain manganese-oxidizing bacteria colonies. This invention solves the problem of altered bacterial physiological metabolism caused by high concentrations of added manganese ions, effectively improving bacterial activity and manganese oxidation capacity.

This invention discloses a low-allergen compound oral liquid for middle-aged and elderly people that protects the liver, stabilizes blood pressure, blood sugar, and blood lipids, combats aging, aids sleep, and prevents Alzheimer's disease. It belongs to the field of food and medicine homology health food technology. The invention uses kudzu root extract, Japanese raisin tree fruit extract, mulberry leaf extract, jujube seed saponins, Acer truncatum seed oil (nervonic acid), blueberry anthocyanins, bitter melon small molecule peptides, wolfberry polysaccharides, γ-aminobutyric acid, and chrysanthemum flavonoids as core active ingredients, refined with a prebiotic system. This invention achieves comprehensive conditioning effects for middle-aged and elderly people through the synergistic action of five pathways: liver protection and metabolism, vascular regulation, anti-oxidation and anti-aging, nerve calming, and brain nerve repair. It simultaneously nourishes and protects the liver, stabilizes blood pressure, blood sugar, and blood lipids, delays aging, improves intractable insomnia, enhances memory, slows brain atrophy, and prevents Alzheimer's disease. This formula strictly avoids eight common allergens, is pure and mild, and contains no irritating additives, making it suitable for long-term use by middle-aged and elderly people with weak constitutions. This product is an oral liquid formulation, with small molecule extraction, strong permeability, rapid absorption, low gastrointestinal burden, stable production process, and high safety. It has extremely high clinical promotion value and industrialization prospects.

The application discloses a kind of based on carbon-based modified electrode microbial quantity dynamic evaluation method.The method comprises the following steps: preparing carbon-based modified electrode doped with target microorganism;Carbon-based modified electrode is used as working electrode, and reference electrode and counter electrode constitute three-electrode system, and is placed in the medium containing target microorganism to be measured in the electrochemical scanning of target microorganism, and the electrochemical response signal is collected;The electrochemical response signal is processed, and the characteristic electrochemical parameter of target microorganism metabolite is obtained;According to the preset detection time threshold selection model: when detection time is less than or equal to preset duration, linear model is used;When detection time is greater than preset duration, dynamic metabolism model is used;Characteristic electrochemical parameter is substituted into the selected model, and the number of microorganisms in the medium to be measured is calculated.The application can realize accurate quantification, anti-interference detection and rapid dynamic evaluation of the number of microorganisms, and significantly improve detection efficiency and accuracy.

This invention discloses the application of α-hydroxyisocaproic acid in weight loss and lipid reduction, belonging to the fields of biopharmaceutical manufacturing, biomedical applications, and functional food development. This invention confirms the application of α-hydroxyisocaproic acid in weight loss; this compound can inhibit weight gain in high-fat diet-induced obese mouse models, reduce body fat, and decrease the area of ​​adipocytes in high-fat diet-induced obese mouse models. Compared to GLP-1 receptor weight-loss drugs that reduce weight by suppressing appetite, the compound α-hydroxyisocaproic acid intervenes in and prevents obesity by accelerating fat metabolism, thereby achieving weight reduction. This invention provides a safe and effective new strategy for weight loss and lipid reduction.

The application discloses an improved plant liquid culture medium, which comprises inorganic nutrients required by plant basic growth and does not comprise compounds containing an EDTA ligand skeleton and iodide. The application also discloses application of the improved plant liquid culture medium in mercury compound metabolism research. The application is based on the technical problem of finding that a culture medium substrate mediates abiotic transformation and causes error defects, and by eliminating the compounds containing the EDTA ligand skeleton and the iodide which induce abiotic transformation of mercury compounds, the application is applied to block the abiotic transformation path of methyl mercury in the mercury compound metabolism research, and eliminate the 'false positive' error in the metabolism research.

The present invention relates to a biosensor that enables the detection of biomarkers related to bone metabolism through saliva samples. This biosensor, which uses a saliva sampling method and offers an economical and user-friendly solution by adopting a non-invasive approach, can detect biomarkers related to bone formation and resorption such as Procollagen Type I N-terminal Propeptide (PINP) and β-C- terminal Telopeptide (β-CTX) in saliva with high sensitivity. The present invention increases patient comfort while also providing rapid analysis, making the diagnosis and treatment processes of diseases related to bone metabolism more efficient.

The application relates to the technical field of health monitoring data analysis, and discloses a cause contribution degree calculation and joint intervention evaluation method and system for sugar homeostasis imbalance, wherein the method comprises the following steps: acquiring a multi-source original monitoring data set; performing a rhythm disturbance basic input set construction task; performing a periodization abnormality representation set construction task; performing a preprocessing task; performing a cause contribution degree time sequence calculation task; performing joint intervention evaluation processing; and outputting a joint intervention evaluation result set. Compared with a scheme for performing coarse-grained evaluation on sugar metabolism abnormalities by relying on a daily average blood glucose index in the prior art, especially under the condition of long-term shift work and circadian rhythm disorder, the technical problem that dynamic migration identification of sugar homeostasis imbalance causes in a continuous time window cannot be realized. Since the application introduces multi-source heterogeneous data alignment modeling under a unified time axis and combines an individual circadian baseline fitting mechanism, the cause explanation capability of sugar homeostasis abnormality evaluation is improved.

The application discloses a kind of low temperature resistant strain YW-1 for preventing and treating rice seedling wilt, and belongs to the field of plant protection.The low temperature resistant strain YW-1 is a pseudo-odor class fungus, and the application proves that the strain YW-1 can maintain normal growth and metabolism in a low temperature environment of 15 DEG C, and can effectively destroy the hyphal structure of the wilt pathogen by secreting cellulase and chitinase, thereby inhibiting the occurrence of the disease from the source.The strain YW-1 has no antagonism with the commonly used functional fungus Trichoderma in the seedling raising substrate, and the two can be compounded and coexist, thereby realizing the synergistic effect of functional microorganisms.The application overcomes the defects of the existing biocontrol strain, such as reduced activity in a low temperature environment and unstable prevention effect, and provides a stable and reliable biological control scheme for factory seedling raising in a low temperature season without increasing the amount of chemical pesticides, thereby significantly improving the quality of seedlings and the seedling raising success rate.

This invention discloses a method for improving the resistance to postharvest black spot disease in sweet potato tubers and maintaining their quality, belonging to the field of postharvest preservation technology for agricultural products. The method includes: selecting undamaged sweet potato tubers for callus treatment at room temperature for 48 hours, disinfecting with 1% sodium hypochlorite, rinsing with sterile water and air-drying, then soaking them in a 9.0 mmol / L benzothiadiazole (BTH) solution (containing 0.05% Tween 80) at room temperature for 60 minutes, removing them and air-drying them to complete the treatment. This invention induces the activation of the sweet potato tuber's own defense system through BTH, increasing the activity of key enzymes in phenylpropane metabolism, chitinase and other disease-resistant enzymes, promoting the accumulation of total phenols and lignin, maintaining cell membrane integrity, inhibiting starch degradation and abnormal increase in soluble sugars. Under storage conditions of 28±1℃ and 80-85%RH, it can significantly inhibit the expansion of black spot disease lesions and extend the storage period to more than 25 days. This method has no pesticide residues, is unlikely to cause pathogen resistance, is environmentally friendly, simple to operate, and cost-controllable, and is suitable for large-scale postharvest preservation of sweet potato tubers.

The application relates to the field of biological medicine, in particular to application of TDF in serum cholesterol regulation and a verification method. The application discloses that tenofovir disoproxil fumarate (TDF) can inhibit intestinal cholesterol absorption by specifically down-regulating the expression level of NPC1L1 protein in the intestinal tract, so as to reduce serum cholesterol. The regulation has tissue specificity, and TDF has no significant influence on the expression of key genes of liver cholesterol metabolism. The application also provides a method for verifying the regulation of TDF on serum cholesterol, which is evaluated by detecting blood lipid indexes and the gene and / or protein expression level of NPC1L1 in duodenal tissue. The application provides a treatment scheme with antiviral and cholesterol-lowering effects for patients with chronic hepatitis B combined with hypercholesterolemia, and provides a new idea for developing a lipid-lowering strategy targeting intestinal cholesterol absorption.

The application provides a copper-nitroimidazole complex which is self-assembled by coordination of copper ions and 2-nitroimidazole. The complex only contains copper ions and 2-nitroimidazole, and the simple two-component combination can realize the chemical kinetics treatment of GSH metabolism enhancement and induce iron death. The copper-nitroimidazole complex has GSH response and hypoxia responsiveness as a nanodrug, and can selectively act in tumor cells. The nanodrug releases copper ions and 2-nitroimidazole in tumor cells, 2-nitroimidazole consumes NADPH under hypoxic conditions to hinder the synthesis of GSH, and copper ions also consume GSH. The consumption of GSH enhances the Cu 2+ / Cu + mediated Fenton reaction to produce active oxygen. 2-nitroimidazole and copper ions self-assemble to form a nanodrug, which greatly improves the loading capacity of 2-nitroimidazole.

This invention discloses a traditional Chinese medicine composition for treating degenerative bone diseases, its preparation method, and its application. It is made from the following raw materials in parts by weight: 30-50 parts Rehmannia glutinosa (processed), 15-30 parts deer antler glue, 20-80 parts Cistanche deserticola, 15-50 parts Morinda officinalis, 15-50 parts tortoise shell glue, 30-80 parts Lycium barbarum, 10-50 parts Curculigo orchioides, 15-50 parts Eucommia ulmoides, 20-80 parts Cuscuta chinensis, and 30-50 parts Dioscorea opposita. 0 parts, Drynaria fortunei 15-50 parts, Achyranthes bidentata 10-50 parts, Taxillus chinensis 20-50 parts, Broussonetia papyrifera 15-30 parts, Clematis chinensis 15-50 parts, Acanthopanax senticosus 15-50 parts; Through the formulation strategy of "regulating the liver and kidneys, nourishing essence and blood, and combining tonification and purging", combined with a specific extraction process, it systematically regulates the balance of bone metabolism, achieves dual prevention and treatment of osteoporosis and bone hyperplasia, and treats both the symptoms and the root cause, and has high safety for long-term use.

This invention provides genes that enhance crop nitrogen metabolism in acidic soils and their applications, belonging to the field of genetic engineering technology. This invention discovers transcription factors... OsART1 It not only participates in regulating the aluminum toxicity response but also integrates the regulation of key processes in nitrogen uptake and assimilation, making it a core regulatory factor connecting acidic soil adaptability with nitrogen metabolism coordination. The transcription factor... OsART1 By directly activating nitrogen metabolism genes such as nitrate reductase OsNR2 The expression of [a specific ingredient] is used to synergistically enhance the nitrogen metabolism capacity and nitrogen use efficiency of rice in acidic soils. This invention [is described in the original text]. OsART1 By enhancing nitrogen assimilation and promoting root growth, it has become a key regulatory factor affecting nitrogen utilization and yield formation in rice under acidic environments.

The present application belongs to the technical field of data processing and intelligent agriculture, and particularly relates to a soil nutrient supply amount prediction method responding to physiological demand, aiming to solve the problems of nutrient supply and demand imbalance, low fertilizer utilization rate and the like caused by ignoring plant physiological metabolism differences. The method comprises collecting environment, soil and plant physiological multi-source data; identifying citrus phenological stages by using a CNN-LSTM hybrid model; constructing a physiological demand index (PDI) model to quantify real-time physiological demand; establishing a soil nutrient bioavailability dynamic conversion model to simulate supply flux; integrating PDI and supply flux, predicting the optimal soil nutrient supply amount through a multi-objective optimization algorithm, and performing closed-loop adaptive correction based on plant physiological response. By using the above technical scheme, the present application realizes the change of nutrient prediction from yield driving to physiological response driving, and significantly improves the fertilizer utilization rate.

Compositions and methods for treating diseases that are responsive to the coadministration of AKG and certain B vitamins are described herein. In addition, compositions and methods for treating host animals that may not respond to AKG monotherapy, including treating host animals having inadequate or dysfunctional expression of enzymes involved in vitamin B metabolism and homeostasis, such as methylenetetrahydrofolate reductase (MTHFR), are described herein.

This invention discloses a fertilization method to increase rice yield, belonging to the field of crop planting technology; it includes: S1: Base fertilizer application, applying a mixed fertilizer solution of compound fertilizer, inhibitor, and water-soluble silicon fertilizer to the roots to form a nitrogen-silicon-inhibitor rhizosphere microdomain; S2: Fertilizer application during the tillering stage, 7-10 days after seedling transplanting, evenly spreading compound fertilizer, and foliar spraying water-soluble silicon fertilizer 3 days after compound fertilizer application; S3: Fertilizer application during the jointing and booting stage, applying compound fertilizer to fields with weak growth from the late tillering stage to the early jointing stage; foliar spraying water-soluble silicon fertilizer on the whole field on the day of fertilization; S4: Fertilizer application during the heading and panicle stage, 3-5 days before heading, during which no compound fertilizer is applied, only water-soluble silicon fertilizer is applied by foliar spraying. This invention solves the existing problem of natural antagonism between high nitrogen fertilization and silicon fertilizer application, and achieves the physiological metabolism synergy between high nitrogen nutrients and active silicon, thus solving the problem that high yield and lodging resistance, and high yield and quality cannot be achieved simultaneously with high nitrogen fertilization.

This invention belongs to the fields of biomedicine and molecular biology, specifically relating to the application of the FER1L6 gene in the prevention and treatment of diabetes. In this application, the expression of GLUT4 is increased by inhibiting the expression of FER1L6, thereby improving the related physiological metabolism in diabetic organisms. This physiological metabolism includes glucose uptake, lipid metabolism, and oxidative stress damage. Experimental results show that FER1L6 is highly expressed in type 2 diabetic mice and insulin-resistant HepG2 cells; reducing FER1L6 expression significantly increases GLUT4 expression, ultimately improving lipid metabolism and oxidative stress damage in diabetic mice, while also enhancing cellular glucose uptake. Therefore, it can be concluded that FER1L6 is a good regulatory target for the prevention and treatment of type 2 diabetes based on the GLUT4 pathway. Based on this target, new technical approaches can be provided for the prevention and control of type 2 diabetes.

This invention discloses a method and apparatus for preserving fresh Chinese medicinal herbs using a combined electric and magnetic field, belonging to the field of post-harvest preservation technology. The method includes: placing cleaned and disinfected fresh Chinese medicinal herbs in a storage device, while simultaneously applying a low-frequency polarized electric field and a static magnetic field; wherein the output voltage of the low-frequency polarized electric field is 3000 V, the current is 1-5 mA, the frequency is 50-60 Hz, and the electric field strength is 25-35 kV / m; the strength of the static magnetic field is 350-450 Gs. This invention effectively regulates cell membrane permeability and free radical metabolism through the spatial synergistic effect of the low-frequency polarized electric field and the static magnetic field, significantly delaying post-harvest decay of Chinese medicinal herbs, inhibiting microbial growth, maintaining the stability of active ingredients, and achieving good preservation effects even at relatively high storage temperatures. It has advantages such as energy saving, being green, easy to operate, and highly applicable, and is suitable for the storage and preservation of various fresh Chinese medicinal herbs.

The invention relates to a system for determining cellular respiration and nutrient metabolism of living cells or tissues, the system comprising: a cell container configured to receive and maintain a biological sample; a gas volume in fluid communication with the cell container, the gas volume containing gaseous components produced by metabolic activity of the biological sample; an optical detection unit configured to detect a target gas species in the gas volume by wavelength-selective absorption spectroscopy, the optical detection unit being further configured to distinguish between different isotopic species of the target gas species, the optical detection unit comprising an illumination source and detector arrangement optically coupled to the optical detection unit and configured for measurement of absorption signals corresponding to different isotopes of the target gas species; and an electronic control and evaluation unit operatively connected to the optical detection unit, the electronic control and evaluation unit being configured to acquire and process absorption data, determine absolute values and / or temporal variations of the target gas species concentration and isotopic composition, and output respiration-related parameters of the biological sample. In this way, a system is provided that enables precise and efficient determination of carbon dioxide produced by living cells or tissues under physiological conditions.

The application provides genes SlSGR1 and SlLCY-B1 for bidirectional regulation of lycopene content in plants and application thereof, relates to the technical field of biotechnology, and discloses application of SlSGR1 protein or related biological materials of the SlSGR1 protein and SlLCY-B1 protein or related biological materials of the SlLCY-B1 protein in regulation of lycopene content in plants. By gene editing, the SlSGR1 protein and the SlLCY-B1 protein are inactivated, the activity of SlPSY1 is improved to promote synthesis, and SlLCY-B1 is inactivated to stop metabolism. SlPSY1 and SlLCY1 interact through PDS, ZDS and CRTISO to improve the content of lycopene. Experiments prove that SlPSY1 and SlLCY-B1 are expressed in fruits, and gene editing does not affect the functions of other genes in the family in stems, leaves and other parts. A new approach for establishing efficient, safe and high-lycopene germplasm is provided.