1703 results about "Metabolism" patented technology

The invention discloses a method for in vitro slow release performance evaluation of a slow and controlled release preparation based on the overflow principle. A container with an upper outlet and a lower outlet is adopted to serve as a release tank, a to-be-detected medicine and a dissolution medium are placed in the release tank, a stirring device is adopted in the release tank to perform stirring, and the dissolution medium is added from the lower outlet of the release tank through a peristaltic pump; the dissolution medium dissolved with the medicine is taken away through the overflow from the upper outlet so as to offset medicine absorption or metabolism, and the slow and controlled release speed of the medicine, the total medicine overflow quantity and the total release amount are calculated by measuring the concentration of the medicine contained in the dissolution medium flowing out, the volume of the release tank and the overflow quantity. According to the method, the releasing degree of the slow and controlled release medicine can be evaluated accurately, the effective medicine release time of the slow and controlled release preparation is evaluated, and slow release performances of the slow and controlled release preparation are evaluated comprehensively.

The invention discloses a clostridium corpse strain and application thereof. The preservation number of the clostridium cadaveris J038 strain of the clostridium cadaveris J038 strain is CGMCC (China General Microbiological Culture Collection Center) NO. 35440, and the clostridium cadaveris J038 strain of the clostridium The clostridium corpse J038 strain is separated from pig manure for the first time, and 4-hydroxyphenylpropionic acid is produced by metabolism of the clostridium corpse J038 strain. In addition, the invention further provides application of the clostridium corpse in preparation of 4-hydroxyphenylpropionic acid, and when the clostridium corpse is adopted to prepare 4-hydroxyphenylpropionic acid, the clostridium corpse has the advantages of being small in environmental pollution, high in sustainability, precise, green, efficient and the like; the yield is high, and the increasing requirements of the fields of medicine, food, agriculture and the like on the 4-hydroxyphenylpropionic acid can be met.

The invention belongs to the technical field of hair care products, and particularly relates to composite acid gel for scalp care and a preparation method thereof. Salicylic acid, glycolic acid, piroctone azelate olamine salt, echinacea purpurea-lactic acid bacteria yeast, an emulsifier and water are combined, wherein the echinacea purpurea-lactic acid bacteria yeast is prepared by performing enzymolysis on echinacea purpurea through bacillus subtilis protease and then performing aerobic fermentation with lactic acid bacteria. Salicylic acid, glycolic acid and azelaic acid in the composite acid gel are compounded, so that stimulation of salicylic acid is weakened, and the effect of dissolving hair follicle grease is reserved; glycolic acid and azelaic acid are added to accelerate keratin metabolism, regulate sebum secretion and create safe and healthy shielding for the scalp environment, and polysaccharide and flavonoid compounds in the echinacea purpurea-lactic acid bacteria leavening are beneficial to exerting the anti-oxidation and immune regulation effects of the echinacea purpurea-lactic acid bacteria leavening. Therefore, the composite acid gel provided by the invention is green, safe and non-irritant, the preparation method is simple and convenient, and the composite acid gel can be used for inhibiting bacteria and reducing scalp inflammation.

The invention provides an in-vitro skin blood vessel immune model as well as a preparation method and application thereof. THP-1 human monocyte leukemia cells are induced to be differentiated into M0 type macrophages, and then the M0 type macrophages are respectively induced into M1 type macrophages and / or M2 type macrophages; then co-culturing the M1 type and / or M2 type macrophages and vascular endothelial cells to form a vascular immune model; finally, the 3D skin model is placed on the blood vessel immune model, external stimulation is conducted, and the in-vitro skin blood vessel immune model is constructed. The in-vitro skin blood vessel immune model can be used for repairing skin barriers, inflammation pathways, blood vessel metabolism, the expression level of genes or proteins related to extracellular matrixes, vascular endothelial cells, the proliferation and migration ability of activated macrophages and the like. The seven feature dimensions of the physiological structure feature of the skin model are used for screening the to-be-tested sample and exploring the action mechanism, and the method has the characteristics of rapidness, multiple screening dimensions, high accuracy, low construction difficulty, low cost and high universality.

The invention discloses a compound probiotic for improving cognitive function of old people and a preparation method and application thereof. Firstly, maltodextrin is dried and then sieved, metal impurities are removed after sieving, and maltodextrin freeze-dried powder, lactobacillus paracasei Lpc37 freeze-dried powder, phytobacterium plantarum Lp-115 freeze-dried powder and bifidobacterium animalis subsp. Lactis Bl-04 freeze-dried powder are weighed and sieved respectively; the preparation method comprises the following steps: preparing freeze-dried powder of three strains, then premixing the freeze-dried powder of the three strains with a part of maltodextrin, adding the residual maltodextrin after the strains are premixed, continuously mixing until the materials are uniformly distributed to obtain the compound probiotics, exerting a synergistic effect through a specific strain combination (Lp115 + Lpc37 + Bl-04), providing a new way for non-drug intervention, being beneficial to regulating intestinal flora metabolism (such as generating short-chain fatty acid SCFA), and improving the immunity of the human body. The synthesis and release of neurotrophic factors are promoted, the nerve cell function is improved, and the cognitive function improvement is indirectly assisted.

The invention provides a cross-omics sparse feature selection system and method based on hierarchical causal modeling, and the system comprises a data input and preprocessing module which is used for receiving multi-omics original data of a multivariate sample; the hierarchical causal structure learning module is connected with the data input and adaptive preprocessing module and is used for constructing a cross-omics hierarchical causal topology; the causal-oriented sparse feature selection module is connected with the hierarchical causal structure learning module; and the model retraining and integration module is used for constructing a three-layer weighted integration discrimination model based on the screened markers, optimizing the fusion weight of each layer through a gradient descent algorithm, and outputting a final prediction result. According to the method, the protein-metabolism biological hierarchy relationship and serum-urine complementary information are fully utilized, and the method has good generalization ability and can be widely applied to marker mining and prediction modeling of cancers, metabolic diseases and the like, so that the accuracy and reliability of precise medical treatment are improved.

The invention provides a composite hydrogel for infectious bone defect repair and a preparation method thereof, and belongs to the technical field of bone defect repair, the composite hydrogel comprises a three-dimensional porous network structure hydrogel and a bioactive unit, and the bioactive unit is loaded in the three-dimensional porous network structure hydrogel; the hydrogel with the three-dimensional porous network structure is prepared from photo-crosslinked methacrylated silk fibroin and nano-hydroxyapatite; the bioactive unit comprises a strontium metal organic framework loaded with curcumin. According to the invention, the photo-crosslinked methacrylated silk fibroin provides excellent biocompatibility; as an ion / drug dual carrier, the strontium metal organic framework can continuously release strontium ions and bidirectionally regulate bone metabolism (promoting osteogenesis and inhibiting osteoclast), and the porous structure of the strontium metal organic framework can efficiently load a hydrophobic drug curcumin (Cur). Curcumin has strong antibacterial and anti-inflammatory functions; the nano-hydroxyapatite can obviously enhance the mechanical strength of the hydrogel.

The invention discloses a humanized osteoporosis mouse model and a construction method thereof, and belongs to the technical field of biotechnology and animal models. The invention aims at the core defects of large species difference, insufficient humanization degree, low pathological simulation degree, unstable phenotype and the like of the existing osteoporosis model. The humanized osteoporosis mouse model is constructed through five core steps of experimental animal pretreatment, collagen-nano hydroxyapatite bionic scaffold preparation and cell pre-implantation, immune-bone metabolism double-derived system construction, progressive osteoporosis induction and model identification and verification. According to the method, double-person origination of immune and bone metabolism systems is realized for the first time, the colonization rate and survival time of human cells are greatly improved, the pathological process of clinical postmenopausal osteoporosis is accurately simulated, the phenotype of the model is stable and uniform, the reactivity matching degree with clinical drugs is high, and the method can be widely applied to research and development of anti-osteoporosis drugs and research of pathological mechanisms.

The invention belongs to the technical field of biology, and particularly relates to application of serine in relieving heat stress of pleurotus ostreatus and a method. Under the heat stress condition, exogenous serine supply promotes pleurotus ostreatus hypha to recover growth, and the activity of antioxidant enzyme, the yield of sporocarp and the yield of nutrient substances are improved; when the activity of the PHGDH enzyme is inhibited, the serine synthesis is reduced; the hypha growth can be promoted by overexpressing the phgdh, and the enzymatic activity of the PHGDH can be improved; when acetyltransferases HAT, NAT1, NAT2 and NAT3 act on a K394 site of the PHGDH, the enzyme activity of the PHGDH can be improved, endogenous serine metabolism is activated to respond to heat stress, it is indicated that PHGDH-mediated serine metabolism plays an important role in corresponding heat stress of the pleurotus ostreatus, and the invention discloses a mechanism for improving heat resistance of the pleurotus ostreatus through the PHGDH-mediated serine metabolism. And a foundation can be laid for breeding high-temperature-resistant pleurotus ostreatus dominant strains.

The invention discloses an application of pleurotus eryngii purified polysaccharide in preparation of an intestinal care medicine. The pleurotus eryngii purified polysaccharide component has high immunocompetence, high-activity and high-purity polysaccharide is obtained after ethanol precipitation and organic solvent purification, and the high biological activity of the polysaccharide is reserved; the composition has the biological activity of increasing the production of short-chain fatty acid, reducing the inflammation level, improving the intestinal flora structure, influencing the body metabolism condition and protecting the intestinal barrier. Compared with other components in the pleurotus eryngii polysaccharide extraction process, the polysaccharide component obtained by the preparation method has the highest purity and the strongest biological activity, and can be used for preparing intestinal care medicines.

The invention relates to the technical field of biological medicine, in particular to a nano-vesicle for targeted brain glioma metabolic immune remodeling and a preparation method and application thereof. According to the nano-vesicle for targeted brain glioma metabolism immune remodeling, double-target metabolism and cascade responsive cleavage site modified biological vesicles are integrated, and effective penetration of a drug blood brain barrier and precise targeting of brain glioma can be achieved. The ROS responsive nanoparticles II are prepared by selecting BSA as a drug carrier, applying a nanoprecipitation method and utilizing SLC1A5, LDHA antagonists and responsive linkers, so that the metabolic double-target inhibitor only responds to specific ROS of tumor cells, and specific release in the tumor cells is realized. The cascade response polypeptide provides a simple and convenient delivery scheme with low invasiveness and application prospects for treatment of brain glioma, and is expected to realize precise and individualized treatment in the field of treatment of malignant glioma.

The invention discloses a diabetes cognitive impairment method based on metabonomics analysis and prediction, and relates to the technical field of biological information, and the method comprises the following steps: S1, obtaining metabonomics data and immunomics data from a peripheral blood sample of a diabetic patient, extracting relevant time sequence data aiming at glucose metabolism, and calculating the glucose metabolism related time sequence data; processing the sequence data by adopting a time sequence analysis algorithm to obtain time sequence change characteristics; s2, constructing a cross-omics interaction network according to time sequence change characteristics, integrating an incidence relation between metabolite concentration and immune factor expression, and setting a dynamic interaction mode; according to the diabetes cognitive impairment method based on metabonomics analysis and prediction, through multi-omics data integration and dynamic network analysis, the precision and reliability of diabetes cognitive impairment mechanism analysis are remarkably improved, and a theoretical basis is provided for precise intervention.

Genetically engineered immune cells, which express at least two metabolism modulating polypeptides and optionally a chimeric receptor polypeptide (e.g., an antibody-coupled T cell receptor (ACTR) polypeptide or a chimeric antigen receptor (CAR) polypeptide) capable of binding to a target antigen of interest. Also disclosed herein are uses of the engineered immune cells for inhibiting cells expressing a target antigen in a subject in need thereof.

The invention discloses a urease-producing microorganism induced calcium carbonate deposition solution for foundation reinforcement and a preparation method and application thereof, and belongs to the technical field of foundation reinforcement. The urease-producing microorganism induced calcium carbonate deposition solution comprises a bacterial solution, a cementing solution and a sodium citrate solution, the bacterial liquid comprises bacteria capable of generating urease and citrobacter freundii. Sodium citrate in the urease-producing microorganism induced calcium carbonate deposition solution can chelate free calcium ions in advance, the concentration of the free calcium ions is reduced, the risk of precipitation at an injection port in the single-phase injection process is eliminated, and the solution can smoothly permeate into a deep layer; citrobacter freundii produces acid through metabolism and utilizes citric acid radicals through metabolism to ensure gradual release of calcium ions, so that uniform deposition of calcium carbonate is triggered. Through the synergistic effect of sodium citrate chelated calcium ions and citrobacter freundii metabolism, the solution can be uniformly distributed in the soil sample, and the problem of uniformity of single-phase perfusion is successfully solved.

The invention belongs to the technical field of biological medicines, and discloses application of vitamin K3 in preparation of a medicine for treating psoriasis. The invention finds that the compound vitamin K3 can obviously inhibit the activation of the STING downstream immune pathway and has an inhibition effect on the activation of the STING pathway. A mouse psoriasis model is formulated according to the absorption and metabolism characteristics of the inhibitor VK3, and it is proved that the VK3 can significantly inhibit the process of psoriasis and up-regulation of an STING pathway at an affected part in a transdermal drug delivery mode. The STING pathway does not belong to a main pathway affecting the course of disease in most non-immunodeficient inflammatory diseases, so that a better treatment effect may be generated by using the STING pathway in combination with a direct treatment drug (such as an IL-17A antibody for psoriasis). The vitamin K3 is simple in chemical structure, mature in production process and low in preparation cost, has more remarkable price advantage compared with biological agents and small-molecule targeting drugs, and has high popularization value and application prospects.

The invention discloses an intelligent purification coating with a bionic pulmonary membrane type respiratory metabolism function. The intelligent purification coating comprises a sensing and adsorption layer, a driving and transportation layer and a catalysis and metabolism unit, the sensing and adsorption layer is composed of a chemical heterojunction network formed by two-dimensional transition metal carbonitride MXene and a zeolite imidazate framework structure material through an in-situ coordination reaction of metal ions and hydroxyl groups on the surface of MXene; the driving and transporting layer takes a cross-linked liquid crystal elastomer network as an intelligent response matrix, and high-length-diameter-ratio magnetic ferroferric oxide nanorods of which the surfaces are modified by a silane coupling agent are uniformly dispersed and embedded into the matrix; and the catalysis and metabolism layer is anchored on a preset key mass transfer path in the driving and transporting layer in a discrete metabolism island form. According to the coating, various volatile organic compounds (VOCs) and enzyme-light synergistic deep mineralization can be realized under mild conditions, and the energy metabolism function of mitochondria can be accurately simulated.

The invention provides a hybrid bionic nano delivery system and a preparation method thereof. The hybrid bionic nano delivery system is composed of a carbon dot doped nitrogen carbide-based nanoparticle core and a cell membrane group wrapping the nitrogen carbide-based nanoparticle core. The invention constructs a hybrid bionic nano delivery system, which has good biocompatibility and biological metabolism, is beneficial to application in the field of biomedicine, and can realize low-temperature photothermal therapy of hepatic fibrosis. The carbon nitride-based nano bionic system constructed by the invention is mainly synthesized by high-temperature calcination, ultrasonic treatment and hybrid cell membrane wrapping. The preparation method disclosed by the invention is simple, has good repeatability and has a relatively good industrial production prospect.

The invention belongs to the technical field of biosynthesis, and particularly relates to a method for regulating and controlling stevioside production by improving the utilization rate of a carbon source. According to the invention, a high-efficiency stevioside biosynthesis system is constructed through systematic engineering modification: in the aspect of chassis cell optimization, transcription factors (AtWRKY18, AtWRKY40 and AtMYC2) for regulating and controlling MEP pathway and key genes (dxs and / or dxr) of the MEP pathway are over-expressed, so that supply of terpene precursors is enhanced; and a heterologous MVA pathway (over-expression T7-atoB-mvaS-mvaE-mvK / mvKmut-pmK-mvaD-idi) with a modular design is introduced, so that sufficient supply of the terpenoid precursor is realized. Meanwhile, by knocking out related genes and bypass metabolism genes (mgsA, iclR, menA or adhE) synthesized by organic acid, a glucose transport and utilization system (fnr, fadR, ppc, zwf or pgi is knocked out, and ACS, fbaA, tpiA, glf, galP or ptsG is overexpressed, so that the utilization rate of a carbon source is further improved, and the production capacity of stevioside is remarkably improved.

The invention discloses a composition for regulating sleep rhythm homeostasis as well as a preparation method and application thereof. The composition for regulating the sleep rhythm homeostasis is prepared from the following components in percentage by weight: 5 to 18 percent of polydextrose, 9 to 15 percent of galactooligosaccharide, 1 to 10 percent of inulin, 0.4 to 2 percent of gamma-aminobutyric acid, 0.8 to 2.2 percent of L-theanine, 0.1 to 3.5 percent of magnesium citrate, 2 to 21 percent of semen ziziphi spinosae extract, 1 to 10 percent of poria cocos extract, 0.5 to 2 percent of pericarpium citri reticulatae extract, 0.3 to 1 percent of liquorice root extract and 15 to 40 percent of maltodextrin. 5-18% of coconut oil powder MCTs, and 0.5-2% of a lily extract. According to the application, a magnesium MCTs shell-core structure is delivered by maltodextrin to form a mitochondrial piezoelectric engine so as to improve prebiotics, promote flora metabolism efficiency and energy supply feedback, activate intestinal pheochromophilic cells and improve SLC6A4 gene expression so as to improve synthesis of 5-hydroxytryptamine in the brain and activate GABA energy neurons; meanwhile, through the spina date seed and poria cocos quantum dots and a pericarpium citri reticulatae and liquorice pi-pi stacking network, the blood-brain barrier penetration rate is greatly increased, and hypothalamic CRH release is adjusted.

The invention relates to the technical field of early warning and monitoring, in particular to a deep groundwater supplementation data analysis and early warning system which comprises a signal processing module, an oxygen consumption analysis module, a nitrogen transmetabolism module, a risk identification module and a coupling optimization module. According to the method, the metabolism trend is analyzed by synchronously collecting underground multi-depth chemical indexes and removing noise, establishing a unified time sequence data set, extracting dissolved oxygen, nitrate and ammonia nitrogen concentration change characteristics, calculating the oxygen dissipation rate and constructing the time-depth distribution relation, and combining nitrogen conversion rate regression. A risk section is identified according to the directions of oxygen dissipation increase and metabolism decrease, the micro-potential gradient and coupling index change are further analyzed in combination with an electrochemical change rule, dynamic identification and risk early warning of the deep groundwater refilling process are achieved, and monitoring is changed from static data comparison to multi-dimensional dynamic analysis. The accuracy and the timeliness of abnormal recognition are improved, and the complement safety and the continuity and the reliability of data results are guaranteed.

The invention discloses an application of rhodococcus diploides in treatment of waste water containing isophorone, the rhodococcus diploides is classified and named as Rhodococcus tectodiphilus, the strain number is GXGD-YZ-2510, and the preservation number is GDMCC No: 67304. The invention further discloses a preparation method of the rhodococcus diploides. The strain shows good growth metabolism activity and isophorone degradation efficiency in a high-salt aerobic environment. Under the conditions that the system salinity is 1 wt%, the initial isophorone concentration is 200 mg / L, and the dissolved oxygen is 3-5 mg / L, the removal rate of the strain on the isophorone reaches 78.6%. The strain not only has excellent salt stress resistance, but also can realize efficient bioconversion of isophorone under high-salt aerobic conditions, and effectively solves the technical bottleneck that isophorone in high-salt wastewater in the industries of petrochemical engineering, chemical engineering, pharmacy and the like is difficult to biochemically treat; the method has the remarkable advantages of being thorough in degradation, low in cost, environmentally friendly, high in process adaptability and the like, and a novel strain resource and an industrialization technical scheme are provided for biological treatment of high-salinity wastewater of refractory organic pollutants.

A Bifidobacterium longum subsp. longum dipro-017. The Bifidobacterium longum subsp. longum dipro-017 used has the ability to metabolize tryptophan to produce indole-3-lactic acid, can inhibit the pathogenic bacteria Salmonella Typhimurium, Staphylococcus aureus, and Escherichia coli, and can effectively mitigate weight loss caused by ulcerative colitis, improve a DAI score, improve colon length, mitigate the content of lipopolysaccharide (LPS) in blood, and increase the level of anti-inflammatory cytokine IL-10, as well as mitigate the content of bone metabolism indicators tartrate-resistant acid phosphatase (TRAP) and N-terminal propeptide of type I procollagen (PINP) in blood.

The present disclosure provides a composition containing a purified population of plant-derived exosome-like nanoparticles isolated from tissue of a vascular plant, wherein the exosome-like nanoparticles comprise a tuned cargo comprising a protein signature and an miRNA signature, wherein the tuned cargo of the plant-derived exosome-like nanoparticles is a result of exposure of the plant to combinations of abiotic stress conditions that cause the plant to modulate its signaling pathways and metabolism to ensure its survival in a challenging environment. The tuned cargo of the plant-derived exosome-like nanoparticles can modulate bioactivities of mammalian cells directly or indirectly. The present disclosure also provides a method for improving appearance of human skin and human hair health, including eyelashes and eyebrows, comprising applying a composition comprising the abiotically stressed plant-derived exosome-like nanoparticles containing the tuned cargo and a carrier; and applying the composition topically.

The invention relates to the technical field of health risk prediction, in particular to a metabonomics-radiomics prediction method for chronic subdural hematoma recurrence risk, which comprises the following steps: acquiring a CT image and extracting edge gray fluctuation, constructing fluctuation parameters in combination with metabolome data, screening coordination characteristics to generate a risk combination, and predicting a chronic subdural hematoma recurrence risk. Feature pairs consistent in trend are extracted to form a collaborative channel, and a feature matrix is constructed to generate an input vector set; according to the method, disturbance features are extracted through a CT image edge gray level path, a cross-modal fluctuation trend comparison mechanism is established in combination with patient brain metabolism indexes, biological consistency between the features is enhanced, feature combinations with uncoordinated changes are eliminated, feature pairs with collaborative structure and function trends are screened, and a linkage path is constructed. The evolution relation from structural disturbance to metabolic response is reflected, channel data sorting and recombination improve the difference of input characteristics, the stability and accuracy of recurrence discrimination are enhanced, and the systematicness and interpretability of risk assessment are improved.

The invention provides a composite herbal cereal protein peptide solid beverage for targeted regulation of uric acid metabolism, and belongs to the technical field of common food developed from medicinal and edible raw materials. According to the invention, Chinese yam, mulberry leaf, gordon euryale seed, lily, coix seed, radish seed, root of kudzu vine, Poria cocos, dried orange peel and other raw materials listed in the catalog of substances which are both food and traditional Chinese medicinal materials in the prior art are carefully selected, and are prepared through scientific compatibility and modern technology. The product not only realizes multi-target reduction of uric acid by inhibiting generation of uric acid and promoting excretion of uric acid, but also is safe and free of toxic and side effects due to the fact that all the components conform to national food safety and medical and edible regulations. A zebra fish in-vivo experiment proves that the uric acid level can be remarkably reduced (Plt; 0.001) under the concentration of 500 mu g / mL. The invention provides a daily food solution integrating the traditional dietary therapy concept and modern scientific verification for people with hyperuricemia.

The invention discloses a construction method of nicotinamide engineering bacteria for cosmetics, which comprises the following steps: amplifying target DNA, recovering the target DNA, carrying out seamless cloning or CPEC connection on the recovered DNA fragment and a vector, carrying out seamless cloning by using a seamless cloning kit of an all-type gold company, carrying out connection by using a sterile ultrapure water complementing system, and carrying out plasmid construction. The method comprises the following steps: inoculating a liquid culture medium with a strain which is verified to be correct, carrying out plasmid extraction on the obtained bacterial liquid by using an AxyPrep plasmid small extraction kit, carrying out shake flask small test on enzyme activity, thallus propagation and protein expression after chassis strain gene editing, adding a substrate 3-cyanopyridine solution into the crushed thallus for reaction, and carrying out HPLC (High Performance Liquid Chromatography) detection on nicotinamide. According to the method, the nitrile hydratase is expressed through metabolism of microorganisms, conversion from 3-cyanopyridine to nicotinamide is achieved through one-step reaction after cell disruption, the enzyme activity of the nitrile hydratase is improved, and meanwhile the generation amount of the by-product nicotinic acid is reduced.

The invention discloses combined probiotics for improving sarcopenia as well as a preparation method and application of the combined probiotics, and belongs to the field of probiotics. The invention relates to a combined probiotic for improving sarcopenia. The combined probiotic comprises a lactobacillus reuteri body, a lactobacillus johnsonii body, a lactobacillus fermentum body and a bifidobacterium adolescentis body. The combined probiotics disclosed by the invention can be used for remarkably improving the overall symptoms of sarcopenia model mice, including inhibiting weight loss, constipation symptoms and colonic mucosa destruction, prolonging load swimming time, improving muscle endurance, improving skeletal muscle atrophy, improving the expression of I-type slow muscle fiber protein MyHC I and promoting slow muscle fiber repair, and has the effects of preventing and treating sarcopenia. The invention discloses a probiotic intervention method for treating sarcopenia, which comprises the following steps of: preparing probiotics for treating sarcopenia, reducing the content of inflammatory cytokines in muscular tissues, increasing the content of hepatic glycogen, improving systematic metabolism of mice with sarcopenia and damaged serum markers, including lactic acid, lactic dehydrogenase, creatine kinase, creatinine, alanine transaminase ALT and aspartate transaminase AST, providing a new scheme for probiotic intervention for sarcopenia, and being used for preparing related functional products or intervention means.

The invention belongs to the field of pet food processing, and relates to a functional pet food for regulating intestinal health and a preparation method thereof. The functional pet food can increase MTL content in dog serum in a physiological range, reduce DAO activity and D-lactic acid and LPS content in dog serum, improve intestinal mucosa barrier function, and significantly improve dog intestinal health. The functional pet food can shorten gastrointestinal transmission time and effectively improve constipation. The functional pet food disclosed by the invention has a good effect in the aspects of treating diarrhea and constipation, and has a two-way regulation effect in regulating intestinal flora imbalance. The components of the raw materials have a synergistic effect, the nutrition is balanced, the preparation method is simple, the palatability is optimally designed, and the metabolic characteristic is free of burden, so that pets can safely take the feed for a long time, a convenient and efficient preventive health management scheme is provided for family pet raising, and the feed has a relatively good commercial application prospect.

The invention discloses a perfusion-free parallel double-cell intestine-liver chip system and application thereof in drug uptake evaluation, and belongs to the field of biomedical engineering and organ chips. The chip disclosed by the invention is simple in structure, free of an external pump and a complex pipeline, capable of realizing fluid circulation through swinging, convenient to operate, low in cost and small in pollution risk, and also capable of promoting cell differentiation and optimizing hepatocyte functions. The chip integrates a parallel intestinal cell and a liver cell which are communicated through a shared channel, can continuously simulate intestinal absorption and liver first-pass metabolism, and is closer to an in-vivo physiological state. The device adopts a detachable transparent modular design, can be repeatedly used, supports multi-group parallel operation, can improve experimental flux and result repeatability, and is suitable for in-vitro verification of drug absorption, permeability and metabolism evaluation.

The invention discloses a method for improving the fermentation performance of clostridium aerovorans and application of the clostridium aerovorans, and according to the method, an effective amount of methionine or a derivative thereof is added into a fermentation culture medium of the clostridium aerovorans to completely replace expensive complex organic nitrogen sources such as yeast extracts. The method successfully solves the three technical bottlenecks of slow biomass accumulation, low ethanol yield and dependence on expensive nitrogen sources in the fermentation process of the clostridium aerovorans by utilizing synthesis gas or CO2 / H2 mixed gas. Experiments prove that methionine or a derivative thereof can synergistically promote growth metabolism of thalli and a synthesis path of a product, so that the gas conversion efficiency of a carbon source is remarkably improved and metabolism is guided to flow to ethanol synthesis while the growth rate and the final biomass of the thalli are improved, and thus the production capacity of alcohols is synchronously enhanced. The method is simple in process and remarkable in effect, and an efficient and economical new strategy is provided for reducing the production cost of biofuel and promoting industrial application of a synthesis gas biorefinery technology.