4981 results about "Metabolism" patented technology

The invention discloses a method for in vitro slow release performance evaluation of a slow and controlled release preparation based on the overflow principle. A container with an upper outlet and a lower outlet is adopted to serve as a release tank, a to-be-detected medicine and a dissolution medium are placed in the release tank, a stirring device is adopted in the release tank to perform stirring, and the dissolution medium is added from the lower outlet of the release tank through a peristaltic pump; the dissolution medium dissolved with the medicine is taken away through the overflow from the upper outlet so as to offset medicine absorption or metabolism, and the slow and controlled release speed of the medicine, the total medicine overflow quantity and the total release amount are calculated by measuring the concentration of the medicine contained in the dissolution medium flowing out, the volume of the release tank and the overflow quantity. According to the method, the releasing degree of the slow and controlled release medicine can be evaluated accurately, the effective medicine release time of the slow and controlled release preparation is evaluated, and slow release performances of the slow and controlled release preparation are evaluated comprehensively.

The invention relates to the technical field of monitoring control, and provides an intelligent monitoring method for a dandelion and golden camellia fermentation process based on the Internet of Things, which comprises the following steps: deploying and self-calibrating a sensor array, extracting and analyzing various data features, and judging a fermentation stage and generating a regulation and control instruction according to the data features. According to the method, a precise fermentation monitoring and control method logic is formed, through omnibearing data acquisition and deep analysis, the change of each parameter in the fermentation process can be precisely grasped, the hypha growth rate, the flora metabolism intensity, the generation of a target product and other key indexes are monitored in real time, the fermentation conditions are adjusted in time, and the fermentation efficiency is improved. And the fermentation process is stabilized in an optimal state, so that the accuracy of fermentation production is greatly improved, the fluctuation of product quality is reduced, and the consistency and stability of the quality of each batch of dandelion and golden camellia are ensured.

The invention discloses an abnormal monitoring method and system for immune cell culture. The method comprises the following steps: collecting cell physical state parameters, culture environment parameters and metabolic biochemical indexes in a culture system in real time; performing motility rate threshold judgment and morphological analysis based on the cell physical state parameters to generate a first abnormal signal; performing dynamic trend analysis on the metabolism biochemical indexes to generate a second abnormal signal; performing grade association on the culture environment parameters, the first abnormal signal and the second abnormal signal, and outputting a monitoring abnormal grade; and performing grading response construction according to the monitoring abnormity grade to obtain an abnormity monitoring report. According to the method, the pollution diffusion risk and the functional cell failure misjudgment rate can be reduced.

The invention discloses a straw feed fermentation treatment control system and method, particularly relates to the technical field of production control, and is used for solving the problem of insufficient identification and regulation of an imbalance phenomenon in a straw fermentation pile caused by non-uniform gas diffusion in the existing fermentation process. The method comprises the following steps: acquiring environmental parameters of a fermentation heap, performing three-dimensional gas concentration field reconstruction processing, identifying a layered air mass region in combination with a fuzzy clustering algorithm, measuring heap pressure distribution and deformation characteristics, analyzing a heap body structure abnormal region, and marking a risk analysis region; through a three-dimensional tensor model and a fractal analysis model, the time dependency and the gas diffusion equilibrium degree of the gas exchange and metabolism process are evaluated respectively, and finally fermentation pile structure parameters and ventilation device operation parameters are dynamically adjusted based on a closed-loop control system; through accurate region identification and a dynamic regulation and control mechanism, the uniformity of the fermentation process and the quality consistency of the straw feed are remarkably improved.

The invention belongs to the technical field of biology, and discloses a reagent combination or kit for constructing intestinal organs and application of the reagent combination or kit. According to the reagent combination or the kit, intestinal organs can be obtained from cell-derived epithelial cells obtained from a donor in a non-invasive manner, and the obtained intestinal organs can be cryopreserved and recovered and can be amplified in vitro for a long time; transcriptome characteristics are similar to those of real human small intestine tissues, and typical marker genes of various small intestine pedigree cell types are highly expressed; compared with intestinal organs obtained through induction of pluripotent stem cells, the intestinal organs have more intestinal pedigree characteristics, and the intestinal function development is more mature; after being promoted to be mature, the intestinal organ also highly expresses genes related to drug absorption and metabolism, has drug absorption capability similar to that of an immortalized intestinal cell line, but more prominently shows intestinal cell lineage characteristics, is closer to an intestinal environment in a real human body, and can be used for screening intestinal disease drugs; the intestinal barrier function is realized.

The invention relates to the technical field of temperature regulation, in particular to an intelligent root temperature regulation system for hydroponic crops and an implementation method thereof, and the system comprises a root system oxygen consumption monitoring module, an osmotic pressure state monitoring module, a root system metabolism state analysis module, a root temperature parameter reconstruction module and a nutrient solution circulation linkage module. According to the method, the metabolic rhythm state of the root system is judged through oxygen consumption rate gradient difference calculation and trend analysis, the cell injury risk is recognized through two-parameter logic judgment by combining the osmotic pressure change amplitude and the oxygen consumption trend, and the target root temperature is selected through gray correlation analysis according to the abnormal triggering temperature and the stable state temperature; a regulation and control instruction is generated, a PID algorithm is called in combination with real-time circulation flow velocity data to adjust the pump speed, closed-loop dynamic matching of a root temperature set value and a flow velocity behavior is achieved, the adaptability of root temperature response is enhanced, the real-time regulation and control capacity for metabolic imbalance is improved, and the stability and the moisture and nutrient absorption efficiency of a water culture environment root system are effectively improved.

The invention relates to a weight-losing enzyme and a preparation method thereof, and belongs to the technical field of biology, and the weight-losing enzyme comprises a fruit enzyme, a vegetable enzyme, a seed enzyme and a lotus leaf extract; the fruit enzyme is obtained by performing enzymolysis on mixed fruit pulp of apples, lemons, grapefruits and haws through pectinase, performing enzymolysis through pepsase and performing co-fermentation through bifidobacterium longum and lactobacillus acidophilus; the vegetable enzyme is obtained by performing enzymolysis on mixed vegetable pulp of wax gourd pulp, celery and spinach through pectinase, performing enzymolysis through pepsin and performing fermentation through lactobacillus plantarum and bifidobacterium adolescentis; the seed enzyme is obtained by carrying out trypsin enzymolysis on mixed powder of semen raphani and phaseolus calcaratus, then carrying out nattokinase enzymolysis and then fermenting with bifidobacterium breve; the lotus leaf extract is obtained by sequentially carrying out enzymolysis on lotus leaves through hemicellulase, snail protease and nattokinase. All the components are matched for use, so that the weight-losing food has the effects of promoting digestion, inhibiting fat absorption, regulating intestinal flora, promoting metabolism and the like, and has a good weight-losing effect.

The invention provides recombinant escherichia coli with high yield of ergothioneine and a method for preparing ergothioneine, the recombinant escherichia coli co-expresses ergothioneine anabolic enzymes Egt1 and Egt2, the methyltransferase structural domain of the Egt1 is derived from trichoderma reesei, mycobacterium smegmatis EgtD or rhizopus nigricans, and the methyltransferase structural domain of the Egt2 is derived from the trichoderma reesei, the mycobacterium smegmatis EgtD or the rhizopus nigricans. The histidine sulfoxide synthase structural domain is derived from trichoderma reesei, schizosaccharomyces pombe or rhizopus nigricans, and the Egt2 is derived from claviceps purpurea, neurospora crassa or aspergillus niger. After the recombinant escherichia coli obtained by screening is cultured for 68 hours in a 5L fermentation tank, the yield of ergothioneine can reach 5g / L or above; wherein after recombinant escherichia coli co-expressing Egt1 enzyme (the structural domain of methyltransferase is derived from mycobacterium smegmatis EgtD, and the structural domain of histidine sulfoxide synthase is derived from trichoderma reesei) and Egt2 enzyme (derived from neurospora crassa) are cultured in a 5L fermentation tank for 68 hours, the yield of ergothioneine can reach 8.7 g / L, and the production intensity reaches 127.9 mg / (L.h).

The present disclosure provides a class of compounds represented by formula (I '), or tautomers, stereoisomers or pharmaceutically acceptable salts thereof. The compound disclosed by the invention has a relatively strong agonistic effect on GLP-1R, can be used for treating diseases such as diabetes mellitus, metabolism-related fatty liver diseases, Alzheimer's disease and the like or has an effect of reducing weight. # imgabs0 #

The invention discloses banana resistant starch as well as a preparation method and application thereof in preparation of health-care food, and relates to the technical field of biology. The preparation method comprises the following steps: peeling immature bananas, performing irradiation disinfection, adding sterile water, performing grinding and pulping, and performing sieving with a 60-mesh sieve so as to obtain slurry; inoculating lactobacillus brevis and kluffa yeast into the slurry, and performing fermentation treatment to obtain fermentation liquor; standing the fermentation liquor, removing the supernatant, cleaning the precipitate with sterile water, and drying to obtain the banana resistant starch. The banana resistant starch prepared by performing combined fermentation treatment on lactobacillus brevis and Kluffa yeast can remarkably enhance the blood glucose metabolism regulation capability under the influence of high fat, so that the banana resistant starch can be applied to preparation of health-care food beneficial to maintaining the blood glucose health level. The method has important practical application prospects and is particularly suitable for market application and popularization.

The invention relates to the technical field of biological medicines, and provides application of an ADRM1 inhibitor in preparation of a medicine for treating osteoporosis. Through the scheme of the invention, the ADRM1 affects bone metabolism, bone tissue morphological structure and osteoporosis progress, and can provide a new drug target for clinical prevention and treatment of osteoporosis. The ADRM1 inhibitor is applied to preparation of the osteoporosis medicine, and bone mass loss can be obviously reduced by down-regulating ADRM1 expression or inhibiting ADRM1 activity and inhibiting differentiation and functions of osteoclasts, so that the effect of treating osteoporosis is achieved.

The biogas in-situ purification device comprises an acid production reactor and a methane production reactor, a discharge port is formed in the bottom of the acid production reactor, the methane production reactor is provided with a feed port connected with the discharge port, a first gas outlet is formed in the top of the methane production reactor, and a second gas outlet is formed in the bottom of the methane production reactor. A gas inlet for directly introducing hydrogen or synthesis gas is formed in the bottom of the methanogenesis reactor, and a conductive material inlet is formed in the acid production reactor and / or the methanogenesis reactor. The conductive material inlet is formed in the acidogenic reactor and / or the methanogenic reactor, and the conductive material is introduced into the system, so that on one hand, the conductive material can promote an interspecific direct electron transfer mode among microorganisms, the tolerance of the acidogenic microorganisms to hydrogen is enhanced, and the overall operation stability of the system is enhanced; and on the other hand, the conductive material can promote the formation of granular sludge in the methane production reactor and enhance the symbiotic metabolism and action among microorganisms, so that the conversion rate of carbon dioxide and the purity of methane in the final biogas are improved.

The invention discloses an intelligent nutrition and metabolism management system for tumor patients, and particularly relates to the technical field of intelligent management. Comprising a patient treatment duration division module, a patient treatment nutrition and metabolism information acquisition module, a patient treatment nutrition intelligent management module, a patient metabolism risk intelligent evaluation module, an auxiliary decision data analysis module, a patient treatment effect intelligent early warning module and a patient treatment nutrition and metabolism intelligent optimization module. According to the invention, the nutrition and metabolism monitoring data of each sub-period of the disease treatment duration of the target patient is collected, the nutrition state evaluation index and the metabolism function risk management index are calculated, and the patient treatment effect early warning index is obtained through analysis, so that the nutrition and metabolism conditions of the patient are intervened, the treatment effect evaluation accuracy is improved, and the patient treatment efficiency is improved. Waste of medical resources is reduced, and intelligent development of treatment effect decision making is promoted.

The invention discloses a vitamin K2 composite micro-capsule system with stomach protection and intestine slow release functions and a preparation method and application thereof, the vitamin K2 composite micro-capsule system with stomach protection and intestine slow release functions comprises three layers, namely a core layer, a middle layer and an outer layer from inside to outside in sequence; the core layer is formed by coating vitamin K2 with a zein-lac hydrophobic core, the middle layer is a pea protein-pectin electrostatic compound, and the outer layer is sodium alginate and chitosan double-network gel. The micro-capsule system provided by the invention can be tightly combined in a gastric acid environment after being orally taken, the release of active ingredients in the stomach is reduced, and then the micro-capsule system stays at intestinal mucosa for a long time and slowly releases contents, so that the functions of the micro-capsule system are slowly presented outside, the half-life period is prolonged, and the action effect is improved. Vitamin K2 directly or indirectly participates in and regulates various physiological processes related to health, and plays an important role in the aspects of maintaining bone health, preventing cardiovascular diseases, protecting nerves, improving metabolism and the like.

The invention provides an application of bifidobacterium animalis subsp. Lactis CP-9 in preparation of a product for promoting bone growth and development or improving bone health. Long-term research and a large number of experiments show that the bifidobacterium animalis subsp. Lactis CP-9 can improve the bone volume fraction, the bone trabecula thickness, the bone density and the bone volume of an organism, and can also effectively improve the growth hormone level and the insulin-like growth factor 1 level, so that bone growth and development are promoted. Meanwhile, the bifidobacterium animalis subsp. Lactis CP-9 can effectively improve the N-terminal propeptide level, osteocalcin level, bone morphogenetic protein level and osteoprotectin level of I-type procollagen in a body, and remarkably reduce the amino-terminal peptide level of the I-type collagen and the ligand level of nuclear factor kappa B receptor activating factor, so that osteoblast differentiation is promoted, osteoclast differentiation is inhibited, and osteoclast differentiation is inhibited. Furthermore, bone formation is promoted, bone resorption is inhibited, bone metabolism is improved, and finally the effect of improving bone health is achieved.

The invention relates to the field of biomedicine, and provides a cardiovascular metabolism risk factor spectrum recognition model construction system, a storage medium and a kit. The recognition model construction system for the cardiovascular metabolism risk factor spectrum comprises an acquisition module, a feature selection module, a training module and a recognition module. The model for predicting the medium-risk or high-risk type of the cardiovascular metabolism risk factor spectrum has excellent risk assessment capability of sensitivity, specificity and accuracy, only needs a small amount of plasma as a detection sample, is low in cost, is easily accepted by a subject, is suitable for large-scale popularization of the method, and has a wide application prospect. The method can also be used for screening cardiovascular metabolism risk factor spectrum drugs.

The invention discloses low-drunkenness type Baijiu distiller's yeast and a preparation method and application thereof. The prepared distiller's yeast can be used for producing baijiu, the physical and chemical indexes of the baijiu are good, fusel oil is reduced by 30%-57%, the metabolism speed of yeast is controlled by controlling the saccharification speed, and the generation of the fusel oil is reduced; meanwhile, lactic acid bacteria and bacillus subtilis of the distiller's yeast and bacteria cultured in a high-temperature stage can effectively inhibit the path of fusel oil metabolized by yeast, generation of fusel oil is further reduced, total ester and total acid are slightly increased basically, the content of total dibasic acid is increased by 10 times, and the content of dibasic acid diethyl ester is increased by 2 times. After the wine liquid is aged, drunkenness detection is carried out, and the comprehensive drunkenness Q is 0.6815-0.8325 and is far lower than a variety of commercially available white spirits (the comprehensive drunkenness is greater than or equal to 1).

The invention discloses a method for restoring soil heavy metal pollution by coupling soybean urease with a charcoal cementing agent, and relates to the technical field of soil heavy metal pollution restoration, and the method comprises the following steps: dynamic microbiome regulation and control: introducing photosensitive protein into soil functional bacteria; the acyl homoserine lactone analogue is synthesized and applied to soil, and is competitively combined with a microbial quorum sensing receptor to block an inhibitor to secrete a signal channel; according to the method, microbial urease secretion is regulated and controlled through optogenetics, an inhibitor signal is blocked through quorum sensing interference, and a microbiome is precisely regulated and controlled; the pH-responsive nanocapsule releases an inhibitor in a targeted manner, and the magnetic-responsive nanochain dynamically captures interfering bacteria, so that the limitation of passive adsorption is broken through; the gene editing plant releases coumarin to inhibit fungal metabolism, and the engineering mycorrhizal fungi physically shields an inhibitor to construct cross-biosphere cooperative defense; the urease activity and the heavy metal removal rate are remarkably improved, meanwhile, the soil microbial diversity is maintained, and the soil specificity interference problem is solved.

The invention belongs to the technical field of drug screening, and provides a multi-target drug screening and optimizing method based on machine learning, which comprises the following steps: S1, carrying out feature screening on compound molecular descriptors by utilizing a machine learning algorithm, and determining the molecular descriptor which has the most significant influence on biological activity; s2, constructing a quantitative structure-activity relation model, and predicting the biological activity of the compound; s3, based on a multi-objective optimization algorithm, combining absorption, distribution, metabolism, excretion and toxicity properties of the compound; according to the method, feature screening is carried out on compound molecule descriptors by utilizing a machine learning algorithm, a QSAR model is constructed to predict the biological activity, ADMET property classification prediction is combined, the biological activity, ADMET properties and pharmacokinetic characteristics of drugs are comprehensively considered, and the accuracy and accuracy of drug activity prediction are improved. The method can efficiently and accurately screen and optimize drug candidate compounds with potential drug effects and good pharmacokinetic characteristics, so that the success rate of drug research and development is increased, and the research and development cost and time are reduced.

The invention relates to the technical field of hemodialysis monitoring, and provides a hemodialysis on-line monitoring system and method, and the system comprises a data acquisition module which is used for collecting blood physiological parameters in a dialysis pipeline in real time; the blood component analysis module is connected with a blood loop of the dialysis machine and is used for detecting serum iron, ferritin and ferritin saturation indexes on line; and the data processing module is used for calculating the risk index of anemia, evaluating the iron utilization rate, constructing a model for measuring the iron deficiency erythropoiesis trend according to the collected and analyzed data, and training and optimizing the model. According to the method, individual parameters of patients are fused, personalized calculation is performed for iron metabolism rates and dialysis parameters of different patients, and data such as iron content, blood flow and dialysis fluid volume are acquired online in real time, so that iron concentration and infusion rate are accurately matched with real-time iron metabolism requirements of the patients, and anemia of the patients in the dialysis process is reduced.

The invention relates to a method for predicting river nitrogen metabolism gene abundance and key influence factors thereof based on automatic machine learning. Comprising the following steps: collecting a river water sample, detecting water quality physicochemical indexes, and performing metagenome sequencing to obtain nitrogen metabolism gene abundance; taking conventional water quality physicochemical indexes as explanatory variables, taking nitrogen metabolism gene abundance as a target variable, adopting automatic machine learning to construct a model, evaluating the performance of the model through a mean square error (MSE) and a fitting coefficient (R), and selecting an optimal algorithm for prediction; the model analyzes the relative contribution of each water quality index through an SHAP value graph, and reveals the relationship between each water quality index and gene abundance by using a partial dependency graph (PDP). The method shows high precision and good generalization performance in the aspect of predicting the abundance of the nitrogen metabolism gene, and is suitable for describing the nitrogen dynamic process of a river water body. The model can explain, analyze and quantify the influence of different water quality indexes on the nitrogen metabolism gene afterwards, and a scientific basis is provided for river water quality management and nitrogen metabolism optimization regulation and control.

The invention relates to a biological preparation for improving quality of weever, the biological preparation comprises an injection and an edible agent, the injection comprises chemically modified chemically synthesized single-stranded small RNA, Rev-erbalpha protein and Per1 protein, and the edible agent comprises Clock core peptide 1, Clock core peptide 2 and taurine. Skeletal muscle development, liver lipid metabolism and generation of cell inflammatory factors of the weever are influenced by adding and injecting the feed, so that the quality of the weever is improved, intramuscular fat deposition is promoted, the taste and flavor of fish meat are improved, the energy reserve of the weever can be improved, and the culture cycle is shortened. The improvement of cell inflammatory factors shows that the disease resistance of the weever is improved, the survival rate in the breeding process is further improved, and the use amount of antibiotics is reduced. The method provides an important theoretical basis for improving fish meat quality in China and promoting high-quality, efficient and healthy development of aquaculture, and has a wide market prospect.

The invention provides a method for biologically removing toxicity inhibition and intensively degrading typical medical organic wastewater under salt stress, and belongs to the technical field of sewage treatment. According to the method, efficient degradation of the high-salt medical organic wastewater is achieved by taking measures such as combination of salt-tolerant nascent-state functional flora and cofactor strengthening, the salt-tolerant nascent-state functional flora comprises obligate COD degradation anaerobic granular sludge and sludge domesticated by gradient salinity pressure of medical wastewater, and the cofactor is betaine, glutamic acid, trehalose or potassium chloride. According to the method, the anaerobic methanogenesis efficiency and TOC removal of organic matters in a degradation system under salt stress can be promoted, the inhibition effect of salt on flora can be relieved and broken through the addition of cofactors, effective metabolism of pollutant nitrogen heterocyclic rings and alkane substances is promoted, refractory characteristic components of wastewater are eliminated, and the biodegradability of the wastewater is improved.

The invention discloses an application of an HERP gene knockout mouse model in obesity and metabolic disorder diseases. The invention finds that compared with a wild type high-fat group, HERP gene knockout can cause phenotypes such as early obesity, more serious obesity degree, increase of fat weight ratio, aggravation of adipose tissue inflammation, dyslipidemia and glucose metabolism disorder of mice during high-fat feeding. And the HERP gene is knocked out, so that the adipocyte differentiation is increased. The HERP gene knockout mouse is more sensitive to nutrition, obesity can occur more quickly during high-fat feeding, symptoms such as fat amplification, fat inflammation and glucose and lipid metabolism disorder are more serious, and the HERP gene knockout mouse can be used for molecular mechanism research and drug screening of obesity, fat tissue and glucose and lipid metabolism disorder. It is further found that cilostazol can significantly relieve mouse obesity and lipid metabolism disorder caused by HERP gene defects, but has no obvious weight losing effect on normal genotype mice, and a new strategy is provided for precise treatment.

The invention relates to the technical field of aplastic anemia (AA) treatment research, discloses a biomarker method for predicting the curative effect of cyclosporine on aplastic anemia, and aims to detect plasma differential metabolites of patients with aplastic anemia by adopting a metabonomics technology, find potential biomarkers for treating AA patients and find the curative effect of cyclosporine on the AA patients. CsA) is used for treating the in-vivo metabolism of AA. The method is based on liquid chromatography-mass spectrometry (LC-MS) and gas chromatography-mass spectrometry (GC-MS) technologies, and comprises the following steps: collecting 5 plasma samples of AA patients, 5 plasma samples of healthy people and 5 plasma samples of patients in a stable period after treatment for comparative analysis; and screening differential compounds by adopting a multivariate statistical method, and further performing enrichment analysis on differential metabolites. Research of the method shows that in-vivo metabolites of AA patients and healthy people are obviously different, and the way related to immunity and energy metabolism may be a key target for treating AA; csA intervenes the occurrence and development of AA by regulating and controlling immune-related metabolic ways.

The invention discloses an intelligent nutrient circulation regulation and control method for a morchella esculenta fruit forest intercropping system based on the Internet of Things, particularly relates to the technical field of intelligent agriculture, and is used for solving the problem of symbiosis imbalance of hyphae and fruit trees caused by nutrient regulation and control lag and static threshold setting in the prior art. Hypha density, electric signal dominant frequency and root system absorption rate data are collected in real time through a multispectral impedance sensor and an ion selective electrode array, and a space-time aligned dynamic matrix is constructed; dynamically updating a nutrient balance threshold value based on a real-time ratio of a hypha metabolism rate to an absorption rate, and generating a nutrient transfer priority by combining collaborative analysis of a hypha transfer direction included angle and an electric signal main frequency offset gradient; a continuous transfer path is identified through a region growing algorithm, water and fertilizer amounts are distributed according to priorities, and a local area instruction is generated; and inverting the equipment response delay by using the high-frequency pulse signal, synchronously adjusting the execution frequency and updating the dynamic matrix and the threshold parameter, thereby realizing the dynamic adaptation and autonomous optimization of the nutrient supply and demand of the intercropping system.

The present invention relates to immunoglobulins, such as immunoconjugates, comprising an ActRII receptor antibody and an agonistic GLP-1 molecule, such as agonistic GLP-1 peptide. Such constructs are useful in the treatment of numerous diseases and disorders, including metabolic disorders, such as obesity, diabetes and others.

The invention relates to the technical field of soil function improvement, in particular to a rice field soil recarburization and capacity expansion method based on a charcoal-based organic fertilizer. The method comprises the following steps: crushing a biomass raw material, pyrolyzing and cooling to obtain biochar; immersing the biochar in a functional bacteria solution containing actinomycetes and bacillus for activating treatment to obtain modified biochar; mixing the modified biochar and decomposed straw, composting and fermenting to obtain organic fertilizers with different particle sizes; the large-particle-size organic fertilizer is applied to deep soil, the soil is covered with soil after straw is laid on the soil, and the small-particle-size organic fertilizer is applied to surface soil. Through layered application, the large-particle-size biochar repairs the deep soil structure and expands the carbon storage capacity, and meanwhile, the carbon stability is improved through carbon-nitrogen synergistic metabolism driven by functional bacteria, so that the carbon immobilization efficiency is improved, meanwhile, soil recarburization and expansion are achieved, the contradiction between carbon sequestration and structure degradation and nutrient imbalance in a traditional recarburization and expansion technology is solved, and the soil recarburization and expansion efficiency is improved. Synergic improvement of a carbon sink function and agricultural productivity is promoted.

The invention discloses a solid waste admixture prepared based on microbiological treatment of regenerated micro-powder and a preparation method, and aims to overcome the defect that the dilution effect is remarkable when regenerated micro-powder is directly used as an admixture in the prior art. The method comprises the following steps: treating waste concrete to obtain regenerated micro powder; culturing bacillus pasteurii, and preparing a bacterial solution with a target OD value; mixing the regenerated micro-powder and a bacterial solution according to a certain solid-to-liquid ratio, adding urea and biochar, and reacting in a magnetic stirrer for a set time to obtain modified regenerated micro-powder; and taking out the modified regenerated micro powder, filtering, drying to constant weight, and fully scattering to obtain the solid waste admixture. According to the present invention, the bacteria-containing culture solution is subjected to centrifugal treatment and the low concentration of the bacteria solution is adopted, such that the by-products generated by the metabolism of the bacillus pasteurii are reduced on the premise of ensuring the sufficient decomposition of the urea, and the prepared regenerated micro-powder contains rich ultrafine calcium carbonate on the surface, and can be adopted as the solid waste admixture to significantly enhance the compressive strength of the cement matrix.

The invention discloses application of Lactobacillus reuteri CCFM1289 with high yield of indoleacetic acid in relieving anxiety and depression, and belongs to the technical field of microorganisms. The Lactobacillus reuteri CCFM1289 can produce heteroauxin at high yield in vivo and in vitro, promote the yield of heteroauxin in fecal flora, relieve anxiety-like and depression-like behaviors of depression mice, relieve HPA hyperfunction, improve the level of brain-derived neurotrophic factor BDNF in hippocampus, promote secretion of 5-HTP and 5-HT in brain, improve the content of heteroauxin in colon, serum and brain, and improve the content of heteroauxin in colon, serum and brain. The compound can be used for improving intestinal flora metabolism imbalance, improving mRNA levels of hippocampal neurogenin-2, synapsin and post-synaptic dense protein 95, maintaining neuronal synaptic function stability, activating AHR signal channels, improving bicortin DCX expression and promoting neurogenesis so as to relieve depression mood, and can be used for drugs, health care products or healthy food for preventing and treating depression.