56 results about "Optogenetics" patented technology

The invention relates to the field of optogenetics, in particular to an optogenetics tool for light-operated induced protein degradation and a construction method and application of the optogenetics tool. The invention relates to an optogenetics tool for light-operated induced protein degradation, which is characterized by comprising a fusion system of a truncated body mTRIM211-80 of mTRIM21 and CRY2, the base sequence of the mTRIM211-80 is as shown in SEQ ID NO. 1 (sequence identifier number 1); the base sequence of the CRY2 is as shown in SEQ ID NO. 2. The optogenetics tool is used for inducing the TRIM21 to form a polymer by utilizing the blue light photosensitive protein CRY2 and is used for the targeted regulation and control of the protein degradation under the driving of the blue light. Targeted degradation of protein can be achieved by utilizing Intrabody (intracellular antibody), time and space targeting is achieved, and after an optogenetics toolbox is optimized, the sensitivity and the reaction rate of the optogenetics toolbox are further improved.

Devices, systems and methods for biological sensing and communication using optogenetics and electronics are described. One example method includes generating a light beam incident on multiple regions in a device, wherein each region comprises an optogenetic system to generate, upon interacting with the light beam, biosensors, wherein an interaction between the biosensors and stimulus molecules in each region is associated with a threshold for a production of an output molecule or an alteration of an output property of the output molecule, the biosensors, or the stimulus molecules, wherein the production or the alteration is based on a value associated with an information source, detecting an output received from one or more of the multiple regions corresponding to the output molecule or the output property in that region, and generating an electric signal associated therewith, and processing the electrical signal to determine the value associated with the information source.

The application belongs to the field of biomedicine, and discloses a method and a kit for monitoring a process of mitochondrion-lysosome interaction-mediated lysosome acidification. The method is characterized in that a cell lysosome content is first labeled with a LYSO-PZ probe with a specific structure, a pHrodo dye and a lysosome membrane marker sfGFP-LAMP1-mCherry are combined to monitor the pH value and membrane localization of the lysosome, respectively, then three-channel fluorescence images are acquired through a structured illumination microscope (SIM), and the fluorescence intensity ratio is quantitatively analyzed. In addition, the method further comprises a step of optogenetic regulation of mitochondrion-lysosome contact (MLC) formation, the fluorescence intensity change before and after the formation of MLC is compared through blue light induction of related protein binding, and the proton transport mediated by MLC is verified. The application first establishes a complete monitoring system, can accurately monitor lysosome acidification, and has important clinical application value and broad market prospect.

Exemplary systems, method and compute-accessible medium can be provided, e.g., for a visual stimulation or an optogenetic activation during a scan of at an object. For example, it is possible to utilize a controller which can be configured to receive signal information regarding the scan, and to precisely control a light engine. The exemplary control of the light engine can be used to activate and deactivate a light source or a plurality of light sources (e.g., one or more at least one light emitting diodes). using the signal information. For example, a digital micromirror device can be utilized through which the light engine is precisely controlled for a full field projection, or patterned projection, wherein the light engine can comprise up to eight high power light sources.

Provided are sequence variants and fusion proteins of the photosensitive ion channel protein ChRmine and their use in treating retinal neurodegenerative diseases. These ChRmine variants and fusion proteins exhibit improved protein properties and enhanced photoresponsive characteristics compared with the wildtype ChRmine, therefore may provide effective optogenetic tools to improve the therapeutic treatment of the retinal neurodegenerative diseases.

The invention discloses an iridium complex as well as a preparation method and application thereof, and relates to the technical field of optogenetics. The iridium complex has the following structural formula, X is an anion, Y is selected from an alkane derivative, an ether derivative or an amine derivative, the iridium complex provided by the invention is regular in structure, the central metal atom iridium has excellent fluorescent property, and fluorine atoms and trifluoromethyl groups introduced into the ligand have excellent fluorescent property, so that the fluorescence intensity of the iridium complex is greatly improved. The electron structure and the energy level of the complex are obviously regulated, the photophysical performance of the complex is enhanced, and excellent two-photon absorption capacity is shown, so that the complex has huge application potential in the fields of biological imaging, photodynamic therapy and the like.

The present invention relates to a two-photon holographic optogenetic stimulation method, system, device, and medium based on wavefront correction. The method comprises: step S1, selecting a target neuron from two-photon imaging and obtaining a hologram corresponding to the three-dimensional spatial position of the target neuron; step S2, selecting a target region on the hologram requiring wavefront distortion correction for the target neuron, and performing wavefront distortion correction on the target neuron in the target region to obtain a phase map containing wavefront distortion information and position information; and step S3, optically exciting the target neuron based on the phase map containing wavefront distortion information and position information. Compared with existing technologies, the present invention achieves deep single-cell resolution excitation of more neurons with a large field of view, lower excitation power, and significantly improves the accuracy and safety of optogenetic stimulation imaging.

The present invention relates to a forebrain assembloid that exhibits maturity similar to that of the human brain and expresses ChR2 to enable optogenetic analysis and, more specifically, to: a method for producing a forebrain assembloid that enables optogenetic analysis; a forebrain assembloid produced by the method; and a forebrain assembloid having a single rosette structure, comprising six cortical layers and cavities, wherein the cortical layers include glial cells and uniformly express ChR2.

The present invention includes methods and systems for optical assays, such as optogenetic assays, of biological activity in which an optical reference signal is used to normalize an optical test signal.

Disclosed are a neural probe, a neural probe interface device connected to the neural probe, and a biological experiment system including the neural probe and the neural probe interface device. The neural probe collects a neural signal generated from neurons based on an optogenetics technique. The neural probe includes a neural probe substrate, an optical input port disposed in the neural probe substrate and configured to receive an optical signal from the outside, an optical output port disposed in the neural probe substrate and configured to transmit the optical signal to neurons, a first electrode configured to receive the neural signal, and a second electrode configured to receive the neural signal from the first electrode through an electric wire and to transmit the neural signal to the outside by using any one of a wired communication method and a wireless communication method.

The present disclosure provides an isolated photosensitive opsin protein that rapidly, reversibly and accurately restores the sensitivity of retinal cells to light by activating Gq signaling.

The utility model provides a wireless photogenetic system of a charging version, which solves the problems that the activity range of an animal is limited and the experimental design is influenced in the existing photogenetic experiment through an animal head connection mode, and the main scheme is that the wireless photogenetic system comprises a control host and a plurality of implantable photoelectric probes, the plurality of implantable photoelectric probes are independently packaged and can be independently controlled through the control host, the plurality of implantable photoelectric probes can be charged through the charging box at the same time, each implantable photoelectric probe comprises an RF chip, an antenna, a battery, a flexible probe substrate and a miniature LED, and the front and back surfaces of the flexible probe substrate are provided with a plurality of groups of joints. The micro LEDs are electrically connected with the connectors in a one-to-one correspondence mode, the battery is electrically connected with the RF chip and the micro LEDs, the RF chip is electrically connected with the micro LEDs and transmits external signals through the antenna, and the control host adjusts output of the micro LEDs through the driving circuit.

The present disclosure provides an isolated photosensitive opsin that rapidly, reversibly, and precisely restores light sensitivity to retinal cells by activating Gq signaling.

Disclosed are methods and compositions for real-time monitoring cellular calcium ion dynamics by red-shifted genetically encoded indicators under optogenetic control, in which millisecond-timescale of temporal control of optical activation or inactivation and signal recording can be achieved. The methods include artifact-free functional imaging in conjunction with optogenetic tools for studying cellular physiology, signal transduction and neuronal activity. Thus, all-optical and non-invasive approaches for drug screening, toxicity testing and assessment of cell functions may be provided.

The application discloses a method for precisely regulating wax synthesis of plant leaves based on light-controlled chloride ion channel protein and belongs to the technical field of genetic engineering. The specific process is as follows: the light-controlled chloride ion channel protein opens or closes the ion channel under irradiation of different light, and the extracellular flow of chloride ions is promoted or terminated, so that the effect of promoting or terminating the wax synthesis is achieved. According to the principle of optogenetics, the application realizes the precise, reversible and space-time controllable regulation of the wax synthesis of the plant leaves, and assists in mining more genes related to the wax synthesis of the plants, thereby providing technical support for improving the adaptation ability of the plants to adversity.

The present invention relates generally to compositions and methods for modulating cell activity using multi-characteristic opsin proteins (MCOs). In addition, the present invention provides methods of treating conditions associated with dorsal root ganglion (DRG), anterior clasps cortex (ACC), or spinal cord activity, including neuropathic pain. Particular embodiments include methods of modulating an inhibitory pathway to inhibit chronic neuropathic pain. The method may use a photosensitive optogenetic actuator with inhibitory neuron specificity. Embodiments also include an optogenetic pain regulator device that can regulate the frequency and intensity of optogenetic stimulation.

The invention belongs to the field of cell culture automation and intelligent regulation and control, and discloses an optogenetics-based adherent cell culture optimization method and system. The method comprises the following steps: inputting an adherent cell type and a culture target, and calling an adherent cell-optical genetic parameter joint database; performing targeted pretreatment on the target adherent cells; processing through a multi-parameter optimization algorithm hardware platform to generate an executable customized photostimulation scheme; performing accurate light stimulation on the positive adherent cells; comparing the obtained detection data with normal adherent cell parameters in a joint database, and evaluating the influence of a photostimulation scheme on the cells; updating the parameters of the multi-parameter optimization algorithm and the prediction model; and inputting the adherent cell type and the culture target to the updated prediction model, and outputting an optimal light stimulation scheme. The core problems of low photostimulation accuracy and poor targeting in the prior art are solved, and high-activity and high-consistency light-sensitive adherent cells are stably produced.

The application discloses an iridium complex and a preparation method and application thereof, and relates to the technical field of optogenetics. The iridium complex has the following structural formula: wherein X is an anion, and Y is selected from alkane derivatives, ether derivatives or amine derivatives. The iridium complex provided by the application has a regular structure, the central metal atom iridium has excellent fluorescence properties, and the fluorine atoms and trifluoromethyl groups introduced in the ligand not only significantly regulate the electronic structure and energy level of the complex, but also enhance the optical physical properties of the complex, and the excellent two-photon absorption capacity is exhibited, which makes the complex have great application potential in the fields of biological imaging and photodynamic therapy.

Training large neural networks on big datasets requires significant computational resources and time. Transfer learning reduces training time by pre-training a base model on one dataset and transferring the knowledge to a new model for another dataset; while current choices of transfer learning algorithms are limited, biological neural networks (BNNs) are adept at rearranging themselves to tackle completely different problems using transfer learning. Taking advantage of BNNs, an artificial neural network (ANN) with dynamic transfer learning capability is transferable to any other network architecture and can accommodate many datasets. The ANN includes artificial neurons and artificial glial cells distributed within an N-dimensional space; connections are formed between pairs of artificial neurons that meet certain criteria. In an optogenetics implementation, machine learning models such as the disclosed ANN are implemented on real BNNs to decrease power consumption.

The invention discloses a fusion protein for regulating and controlling functions of pancreatic beta cells based on an optogenetics technology and application of the fusion protein, and relates to the technical field of biology. The fusion protein Opto LOVTRAP disclosed by the invention has very high temporal-spatial resolution, and rapid and accurate control on a single cell can be realized by adjusting blue light; opto LOVTRAP has reversibility, can effectively inhibit a pancreatic beta cell Ca < 2 + > channel under a dark condition, and can quickly stop playing a role after being stimulated by blue light, so that the purpose of reversibly and accurately regulating insulin secretion is achieved. Opto LOVTRAP is targeted to regulate the concentration of Ca < 2 + > in cells, and is a final triggering factor for release of pancreas islet vesicles, so that the opto LOVTRAP is not influenced by the KATP function, and the purpose of breaking through the bottleneck of a traditional clinical treatment mode is achieved.

To provide a method that enables laser light emitted from a plurality of light sources to be simultaneously radiated easily, in applications such as optogenetics in which laser light is radiated onto an observation sample of an optical microscope.SOLUTION: The present invention relates to a system connectable to a camera port of a microscope, including: an imaging sensor capable of capturing an image of a sample; two light sources; and two movable mirrors for adjusting positions at which the observation sample is irradiated with focused spots of laser light emitted from the light sources, and also relates to a method for laser irradiation using the system.SELECTED DRAWING: Figure 2

Provided are methods to increase the nephron yield and control over nephron locations in kidney organoids and kidney replacement tissues; the methods achieve spatiotemporal control over the mechanical microenvironment to engineer favorable environments for nephron formation within kidney organoids. This engineered control can be created using microdevices that impose mechanical stress at defined intervals synchronized with cyclical nephron development in vivo, agonists / antagonists of tension-generating biochemical pathways in whole organoids or cells, optogenetic control over tension in stem cell collectives, and / or cell-cell junction mediated transfer of mechanical information between cells, or similar approaches.

Recombinantly produced fusion proteins are provided that include an optically responsive element fused to a downstream signaling element derived from an active domain of a signaling molecule, e.g., an intracellular domain of a receptor protein, and thus are able to be activated by light exposure instead of the natural activation event of the signaling molecule. A signal transduction cascade reaction is triggered, such as binding of a ligand to an acceptor. Nucleic acids encoding the fusion proteins and host cells containing the fusion proteins or nucleic acids are also provided. Also provided are methods of using light exposure to achieve desired downstream effects of signal transduction pathways that are typically mediated by signal transduction molecules, e.g., in promoting retinal ganglion cell proliferation, survival and / or regeneration.

Provided herein are methods and systems for growing and maintaining stem cells in suspension. In some cases, the methods and systems involve the use of optical switches for activating one or more growth factor signaling pathways involved in the growth and / or maintenance of stem cells.

The invention discloses a regulation and control system and method for living tissue cells, relates to the field of biomedicine, can regulate and control specific cells in a deep area to be regulated and controlled in living tissue, and comprises a laser source module, a modulation module, an optical fiber module, an imaging module and a control module, the modulation module modulates the received first laser based on a preset modulation strategy to determine regulation and control light, and modulates the received second laser based on the preset modulation strategy to determine imaging light, the first laser is used for regulating and controlling target cells based on optogenetics, the second laser is used for imaging a target area, and the imaging light is used for imaging the target area. The target area refers to a to-be-regulated area in a living body tissue where target cells are located; the regulation and control light or the imaging light is output to a deep layer area of a living body tissue through a multimode optical fiber in the optical fiber module, the system firstly images a target area through the imaging light, determines a target regulation and control area where a target cell is located, and then precisely regulates and controls the target cell through the regulation and control light.

The application discloses a kind of multi-wavelength array type light regulation and detection device for optical genetics, belong to the field of bioengineering and automatic control, comprising: array light source, mechanical control platform, imaging device and control module;Mechanical control platform is provided with microwell plate, and the microwell plate includes a plurality of array culture wells for cell culture;Control module is connected with array light source;Array light source outputs a plurality of groups of sub-beams distributed in array, and the position of the plurality of groups of sub-beams corresponds to the culture well of different position on microwell plate respectively;The light of each group of sub-beams has different target parameters, including wavelength, illumination intensity and time.The multi-wavelength array type light regulation and detection device for optical genetics disclosed in the application can be used to construct multi-wavelength array type light regulation experimental platform with controllable illumination intensity and time, and further tap the potential of optical genetics in cell high-throughput metabolic regulation.

The invention provides a variable-diameter square-core optical fiber structure which is based on frontier requirements of a lensless optical fiber microscopic imaging technology and aims at solving the contradiction between low invasiveness and high resolution. The variable-diameter square-core optical fiber structure is characterized by comprising a near-end square core 1, a far-end square core 2, a variable-diameter square core 3, a wrapping layer 4, a protective layer 5 and a near-end titanium alloy reinforced micro sleeve 6, the radial cross section of the core layer is rectangular, the length of the rectangle is a, the width of the core layer is b, the length and the width of the rectangle are in a certain proportion, the relative refractive index difference delta of the core layer needs to be small enough, GeO of the core layer is doped, a cladding junction material is pure SiO, the variable diameter length is 20 mm, heat insulation transmission is ensured, a protective layer material is polyimide, and a titanium alloy reinforced micro sleeve is adopted for near-end reinforcement. And the fiber core is prevented from being broken in tissues. The method can be widely applied to a low-damage and high-resolution endoscopic imaging technology, and promotes the development and application of a lensless optical fiber microscopic imaging technology in the field of micro-scale life science such as cranial neuroscience, optogenetics and unicellular biology.

According to the diagnosis and treatment integrated implantable hydrogel optical fiber probe and the preparation method thereof, firstly, flexible hydrogel is selected on the aspect of materials, polyvinyl alcohol is used as a base material, and damage to tissues caused by the problem of mechanical mismatching is reduced; in order to better utilize the optogenetics effect, the probe is designed into a core-shell structure consisting of a fiber core with a high refractive index and a cladding with a low refractive index, so that better optical transmission is carried out; secondly, in order to meet the requirement of multiple functions, a PVA-CNTs electrode is integrated in the optical fiber for electric recording; meanwhile, the micro-channel is also designed in the optical fiber, a hollow channel is formed in a mode of sacrificing the sodium alginate fiber, so that the medicine is conveyed, and finally, the flexible implantable hydrogel optical fiber probe integrating three functions of light guide, electric conduction and medicine conveying is prepared. Diversification of functions is realized while damage to tissues caused by mechanical mismatching is reduced, so that the medical instrument is applied to nervous system diseases, such as epilepsy and Parkinson's disease.