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22 results about "Optogenetics" patented technology
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Optogenetics (from Greek optikós, meaning 'seen, visible') is a biological technique that involves the use of light to control cells in living tissue, typically neurons, that have been genetically modified to express light-sensitive ion channels. It is a neuromodulation method that uses a combination of techniques from optics and genetics to control and monitor the activities of individual neurons in living tissue—even within freely-moving animals—and to precisely measure these manipulation effects in real-time. The key reagents used in optogenetics are light-sensitive proteins. Neuronal control is achieved using optogenetic actuators like channelrhodopsin, halorhodopsin, and archaerhodopsin, while optical recording of neuronal activities can be made with the help of optogenetic sensors for calcium (GCaMP), vesicular release (synapto-pHluorin), neurotransmitter (GluSnFRs), or membrane voltage (arc lightning, ASAP1). Control (or recording) of activity is restricted to genetically defined neurons and performed in a spatiotemporal-specific manner by light.
Biological sensing and communication using optogenetics and electronics
Devices, systems and methods for biological sensing and communication using optogenetics and electronics are described. One example method includes generating a light beam incident on multiple regions in a device, wherein each region comprises an optogenetic system to generate, upon interacting with the light beam, biosensors, wherein an interaction between the biosensors and stimulus molecules in each region is associated with a threshold for a production of an output molecule or an alteration of an output property of the output molecule, the biosensors, or the stimulus molecules, wherein the production or the alteration is based on a value associated with an information source, detecting an output received from one or more of the multiple regions corresponding to the output molecule or the output property in that region, and generating an electric signal associated therewith, and processing the electrical signal to determine the value associated with the information source.
Owner:WISCONSIN ALUMNI RES FOUND
Systems, methods and computer-accessible medium for visual stimulation and optogenetics
Exemplary systems, method and compute-accessible medium can be provided, e.g., for a visual stimulation or an optogenetic activation during a scan of at an object. For example, it is possible to utilize a controller which can be configured to receive signal information regarding the scan, and to precisely control a light engine. The exemplary control of the light engine can be used to activate and deactivate a light source or a plurality of light sources (e.g., one or more at least one light emitting diodes). using the signal information. For example, a digital micromirror device can be utilized through which the light engine is precisely controlled for a full field projection, or patterned projection, wherein the light engine can comprise up to eight high power light sources.
Owner:THE TRUSTEES OF COLUMBIA UNIV IN THE CITY OF NEW YORK
Variants of the photosensitive ion channel protein chrmine and uses thereof in treating retinal neurodegenerative diseases
Provided are sequence variants and fusion proteins of the photosensitive ion channel protein ChRmine and their use in treating retinal neurodegenerative diseases. These ChRmine variants and fusion proteins exhibit improved protein properties and enhanced photoresponsive characteristics compared with the wildtype ChRmine, therefore may provide effective optogenetic tools to improve the therapeutic treatment of the retinal neurodegenerative diseases.
Owner:SUZHOU UGENEX THERAPEUTICS CO LTD
Neural probe, neural probe interface device connected to the neural probe, and biological experiment system including the neural probe and the neural probe interface device
Disclosed are a neural probe, a neural probe interface device connected to the neural probe, and a biological experiment system including the neural probe and the neural probe interface device. The neural probe collects a neural signal generated from neurons based on an optogenetics technique. The neural probe includes a neural probe substrate, an optical input port disposed in the neural probe substrate and configured to receive an optical signal from the outside, an optical output port disposed in the neural probe substrate and configured to transmit the optical signal to neurons, a first electrode configured to receive the neural signal, and a second electrode configured to receive the neural signal from the first electrode through an electric wire and to transmit the neural signal to the outside by using any one of a wired communication method and a wireless communication method.
Owner:ELECTRONICS & TELECOMM RES INST
Optogenetic vision recovery with photosensitive gq coupling neurooptin (optin 5)
Owner:GENANS BIOTECHNOLOGY CO LTD
Genetically encoded fluorescent indicators under optogenetic control and uses thereof
Disclosed are methods and compositions for real-time monitoring cellular calcium ion dynamics by red-shifted genetically encoded indicators under optogenetic control, in which millisecond-timescale of temporal control of optical activation or inactivation and signal recording can be achieved. The methods include artifact-free functional imaging in conjunction with optogenetic tools for studying cellular physiology, signal transduction and neuronal activity. Thus, all-optical and non-invasive approaches for drug screening, toxicity testing and assessment of cell functions may be provided.
Owner:LUMISTAR BIOTECHNOLOGY INC +1
A method for precisely regulating wax synthesis based on light-controlled chloride channel protein
ActiveCN120836317BIon Channel ProteinOptogenetics
The application discloses a method for precisely regulating wax synthesis of plant leaves based on light-controlled chloride ion channel protein and belongs to the technical field of genetic engineering. The specific process is as follows: the light-controlled chloride ion channel protein opens or closes the ion channel under irradiation of different light, and the extracellular flow of chloride ions is promoted or terminated, so that the effect of promoting or terminating the wax synthesis is achieved. According to the principle of optogenetics, the application realizes the precise, reversible and space-time controllable regulation of the wax synthesis of the plant leaves, and assists in mining more genes related to the wax synthesis of the plants, thereby providing technical support for improving the adaptation ability of the plants to adversity.
Owner:ZHENGZHOU UNIV
Optogenetic-based adherent cell culture optimization method and system
PendingCN121963840Ahigh activityimprove consistencyInstrumentsMolecular structuresOptogeneticsBiomedical engineering
The invention belongs to the field of cell culture automation and intelligent regulation and control, and discloses an optogenetics-based adherent cell culture optimization method and system. The method comprises the following steps: inputting an adherent cell type and a culture target, and calling an adherent cell-optical genetic parameter joint database; performing targeted pretreatment on the target adherent cells; processing through a multi-parameter optimization algorithm hardware platform to generate an executable customized photostimulation scheme; performing accurate light stimulation on the positive adherent cells; comparing the obtained detection data with normal adherent cell parameters in a joint database, and evaluating the influence of a photostimulation scheme on the cells; updating the parameters of the multi-parameter optimization algorithm and the prediction model; and inputting the adherent cell type and the culture target to the updated prediction model, and outputting an optimal light stimulation scheme. The core problems of low photostimulation accuracy and poor targeting in the prior art are solved, and high-activity and high-consistency light-sensitive adherent cells are stably produced.
Owner:BEIJING TONGREN HOSPITAL AFFILIATED TO CAPITAL MEDICAL UNIV
Artificial neural network with dynamic transfer learning capability and optogenetic implementation
Training large neural networks on big datasets requires significant computational resources and time. Transfer learning reduces training time by pre-training a base model on one dataset and transferring the knowledge to a new model for another dataset; while current choices of transfer learning algorithms are limited, biological neural networks (BNNs) are adept at rearranging themselves to tackle completely different problems using transfer learning. Taking advantage of BNNs, an artificial neural network (ANN) with dynamic transfer learning capability is transferable to any other network architecture and can accommodate many datasets. The ANN includes artificial neurons and artificial glial cells distributed within an N-dimensional space; connections are formed between pairs of artificial neurons that meet certain criteria. In an optogenetics implementation, machine learning models such as the disclosed ANN are implemented on real BNNs to decrease power consumption.
Owner:UVIC INDUSTRY PARTNERSHIPS INC
Fusion protein for regulating pancreatic beta cell function based on optogenetics technology and application of fusion protein
The invention discloses a fusion protein for regulating and controlling functions of pancreatic beta cells based on an optogenetics technology and application of the fusion protein, and relates to the technical field of biology. The fusion protein Opto LOVTRAP disclosed by the invention has very high temporal-spatial resolution, and rapid and accurate control on a single cell can be realized by adjusting blue light; opto LOVTRAP has reversibility, can effectively inhibit a pancreatic beta cell Ca < 2 + > channel under a dark condition, and can quickly stop playing a role after being stimulated by blue light, so that the purpose of reversibly and accurately regulating insulin secretion is achieved. Opto LOVTRAP is targeted to regulate the concentration of Ca < 2 + > in cells, and is a final triggering factor for release of pancreas islet vesicles, so that the opto LOVTRAP is not influenced by the KATP function, and the purpose of breaking through the bottleneck of a traditional clinical treatment mode is achieved.
Owner:ZHEJIANG UNIV
Diagnosis and treatment integrated implantable hydrogel optical fiber probe and preparation method thereof
According to the diagnosis and treatment integrated implantable hydrogel optical fiber probe and the preparation method thereof, firstly, flexible hydrogel is selected on the aspect of materials, polyvinyl alcohol is used as a base material, and damage to tissues caused by the problem of mechanical mismatching is reduced; in order to better utilize the optogenetics effect, the probe is designed into a core-shell structure consisting of a fiber core with a high refractive index and a cladding with a low refractive index, so that better optical transmission is carried out; secondly, in order to meet the requirement of multiple functions, a PVA-CNTs electrode is integrated in the optical fiber for electric recording; meanwhile, the micro-channel is also designed in the optical fiber, a hollow channel is formed in a mode of sacrificing the sodium alginate fiber, so that the medicine is conveyed, and finally, the flexible implantable hydrogel optical fiber probe integrating three functions of light guide, electric conduction and medicine conveying is prepared. Diversification of functions is realized while damage to tissues caused by mechanical mismatching is reduced, so that the medical instrument is applied to nervous system diseases, such as epilepsy and Parkinson's disease.
Owner:DONGHUA UNIV
Integrated light source biocell culture sensing device
PendingCN122168411ABioreactor/fermenter combinationsBiological substance pretreatmentsOptogeneticsLight spot
This invention discloses an integrated light source biological cell culture sensing device. The device comprises a Micro-LED array and a biosensor array, which are bonded together. A DGHFET device and the Micro-LED device form a field-effect biosensor, with the DGHFET device located within the light spot coverage area of the Micro-LED device within the same field-effect biosensor. This integrated light source biological cell culture sensing device successfully integrates efficient photostimulation, high-sensitivity biosensing, and high-reliability anti-interference capabilities onto a single platform, providing a powerful, reliable, and flexible integrated solution for optogenetic research. This invention has wide applications in the semiconductor technology field.
Owner:SUN YAT SEN UNIV
Optogenetic system promoting targeted homing of mscs to damaged liver, and preparation method therefor
An optogenetic system promoting the targeted homing of MSCs to a damaged liver, and a preparation method therefor. On the basis that VUR8 exhibits a dimer structure in a natural state and dissociates following UVB irradiation, an optogenetic system comprising light-controlled CXCR4-releasing plasmids which contain UVR8 and CXCR4 sequences and upconversion nanoparticles which can convert NIR having strong tissue penetration capability into UVB is developed, and the optogenetic system is used for implementing the overexpression of CXCR4 only on MSC cells in the liver region so as to promote the targeted homing effect thereof.
Owner:NANJING DRUM TOWER HOSPITAL
Optogenetics tool for light-operated induced protein degradation as well as construction method and application of optogenetics tool
The invention relates to the field of optogenetics, in particular to an optogenetics tool for light-operated induced protein degradation and a construction method and application of the optogenetics tool. The invention relates to an optogenetics tool for light-operated induced protein degradation, which is characterized by comprising a fusion system of a truncated body mTRIM211-80 of mTRIM21 and CRY2, the base sequence of the mTRIM211-80 is shown as SEQ ID NO. 1, and the base sequence of the mTRIM211-80 is shown as SEQ ID NO. The base sequence of the CRY2 is as shown in SEQ ID NO. 2. The optogenetics tool is used for inducing the TRIM21 to form a polymer by utilizing the blue light photosensitive protein CRY2 and is used for the targeted regulation and control of the protein degradation under the driving of the blue light. Targeted degradation of protein can be achieved by utilizing Intrabody (intracellular antibody), time and space targeting is achieved, and after an optogenetics toolbox is optimized, the sensitivity and the reaction rate of the optogenetics toolbox are further improved.
Owner:ZHEJIANG UNIV OF TECH +1
OPTOGENETIC ALPHA-SYNUCLEIN AGGREGATION SYSTEM-BASED COMPOUND SCREENING PLATFORM IN PD-hiPSC-mDA NEURONS
Provided herein are methods and compositions for identifying α-synuclein aggregation inhibitors. Also provided are methods of use of the α-synuclein aggregation inhibitors; the methods include methods of inhibition the formation of Lewi bodies and methods of treating synucleinopathies in subjects. Methods are compositions provided herein include optogenetic α-synuclein fusion proteins and an optogenetic alpha-synuclein (α-syn) aggregation system. Further, provided herein are compositions comprising α-synuclein aggregation inhibitor drug candidates identified using an optical alpha-synuclein aggregation screening system. The α-synuclein aggregation inhibitor drug candidates have neuroprotective effects in vitro and in vivo and provide proof-of principle that the optical alpha-synuclein aggregation screening system can be used to identify drug candidate for synucleopathies and tauopathies, including for example Parkinson's disease.
Owner:JOHNS HOPKINS UNIVERSITY
A fusion protein based on optogenetics to regulate endogenous ABA signaling and its applications
This invention belongs to the field of plant regulation and protein engineering technology, specifically relating to a fusion protein that regulates endogenous ABA signaling based on optogenetics and its applications. Specifically, this invention, based on optogenetics, fuses the protein kinase OST1 from the ABA signal transduction component with the optogenetic element cpLOV2, which can be activated / inactivated through dark / light treatment. This achieves the goal of using light to transmit and block ABA signals within the cell to regulate stomatal movement, thereby increasing plant biomass and achieving high and sustainable crop yields, thus possessing significant practical application value.
Owner:HENAN UNIVERSITY
Method for realizing synthesis of specific membrane-free organelles by utilizing optogenetics technology and application
PendingCN122060075ARegulating the dynamic assembly processovercome limitationsPolypeptide with localisation/targeting motifAntibody mimetics/scaffoldsOptogeneticsIntracellular
The invention relates to a method for realizing synthesis of a specific membrane-free organelle by utilizing an optogenetics technology and application, and belongs to the technical field of biology. The method comprises the following steps: firstly, screening a recombinant protein for specifically synthesizing the membrane-free organelle, transfecting the recombinant protein into a to-be-observed cell, and accurately regulating and controlling the dynamic assembly process of the membrane-free organelle in the cell in real time by utilizing a light-operated tool. The method not only avoids the limitation of static observation of a fixed cell technology, but also overcomes the problems of large interference of a fluorescence labeling and living cell imaging technology on an intracellular environment and inaccurate dynamic observation, and provides a powerful technical support for deeply researching abnormal behaviors of neurodegenerative disease related proteins in a membraneless organelle and a disease occurrence mechanism.
Owner:SUZHOU UNIV
Thalamic input drive to orbitofrontal cortex is mediated by GABA and is frequency dependent across the brain
Provided herein are methods and systems for modulating the temporal patterns of neuronal activity in the brain. The methods of the present disclosure can include using optogenetics to stimulate one or more of thalamocortical projections in the brain, thalamic relay neurons, cortical projection neurons, cell bodies in the centrolateral nucleus of the thalamus, and cell bodies in the VLO, in conjunction with fMRI of different regions of the brain to directly visualize the global impact of afferent and efferent connections of the VLO, and to characterize how different temporal patterns of activity in the VLO circuit affect brain dynamics by driving its inputs and outputs at different frequencies.
Owner:THE BOARD OF TRUSTEES OF THE LELAND STANFORD JUNIOR UNIV
A core-sheath structure hydrogel optical fiber array system and a preparation method and application thereof
Owner:SICHUAN UNIV +1
Brassica napus genetic transformation and identification method for optogenetics
The invention discloses a brassica napus genetic transformation and identification method for optogenetics, and relates to the technical field of plant genetic transformation. According to the method, red light is innovatively adopted as culture illumination for brassica napus genetic transformation, the wavelength range can accurately avoid an activation spectrum of a non-red light activation optogenetics tool, the tool is fundamentally prevented from being mistakenly activated in the culture process, the problem of callus browning is thoroughly solved, and it is guaranteed that the transformation process is smoothly advanced. The core logic of the constructed red light adaptive genetic transformation system comprises illumination selection, culture medium optimization, parameter regulation and control and identification standardization, the red light adaptive genetic transformation system can be directly migrated to other crops, and a mature framework is provided for application of a non-red light activated optogenetics tool in multiple crops; the prior art is designed only for white light conversion of rape and has no cross-crop application potential.
Owner:ZHENGZHOU UNIV
Cell division promoting factor FL1 / FL3 specifically interacting with non-light excited state CRY2 and application of cell division promoting factor FL1 / FL3
The invention provides a cell division promoting factor FL1 / FL3 capable of specifically interacting with non-light excited state CRY2 and application of the cell division promoting factor FL1 / FL3. The invention discloses an interaction system for the first time, and the system comprises CRY2 and FL1 and / or FL3 which specifically interact with the CRY2 in a non-light excitation state. The invention also provides application of the interaction system as a plant trait regulation target and an optogenetics core element, wherein the trait comprises a cell division related trait or a root length related trait.
Owner:SHENZHEN UNIV