98 results about "Optogenetics" patented technology

The invention discloses a method for restoring soil heavy metal pollution by coupling soybean urease with a charcoal cementing agent, and relates to the technical field of soil heavy metal pollution restoration, and the method comprises the following steps: dynamic microbiome regulation and control: introducing photosensitive protein into soil functional bacteria; the acyl homoserine lactone analogue is synthesized and applied to soil, and is competitively combined with a microbial quorum sensing receptor to block an inhibitor to secrete a signal channel; according to the method, microbial urease secretion is regulated and controlled through optogenetics, an inhibitor signal is blocked through quorum sensing interference, and a microbiome is precisely regulated and controlled; the pH-responsive nanocapsule releases an inhibitor in a targeted manner, and the magnetic-responsive nanochain dynamically captures interfering bacteria, so that the limitation of passive adsorption is broken through; the gene editing plant releases coumarin to inhibit fungal metabolism, and the engineering mycorrhizal fungi physically shields an inhibitor to construct cross-biosphere cooperative defense; the urease activity and the heavy metal removal rate are remarkably improved, meanwhile, the soil microbial diversity is maintained, and the soil specificity interference problem is solved.

Hydrogen-bonded framework nanoparticles comprising a chemiluminescent compound (e.g., L-012) are provided and can be used, e.g., for sono-optogenetic treatment of neurodegenerative diseases such as Parkinson's disease. Related in vivo and therapeutic methods are also provided.

The invention relates to the field of optogenetics, in particular to an optogenetics tool for light-operated induced protein degradation and a construction method and application of the optogenetics tool. The invention relates to an optogenetics tool for light-operated induced protein degradation, which is characterized by comprising a fusion system of a truncated body mTRIM211-80 of mTRIM21 and CRY2, the base sequence of the mTRIM211-80 is as shown in SEQ ID NO. 1 (sequence identifier number 1); the base sequence of the CRY2 is as shown in SEQ ID NO. 2. The optogenetics tool is used for inducing the TRIM21 to form a polymer by utilizing the blue light photosensitive protein CRY2 and is used for the targeted regulation and control of the protein degradation under the driving of the blue light. Targeted degradation of protein can be achieved by utilizing Intrabody (intracellular antibody), time and space targeting is achieved, and after an optogenetics toolbox is optimized, the sensitivity and the reaction rate of the optogenetics toolbox are further improved.

Devices, systems and methods for biological sensing and communication using optogenetics and electronics are described. One example method includes generating a light beam incident on multiple regions in a device, wherein each region comprises an optogenetic system to generate, upon interacting with the light beam, biosensors, wherein an interaction between the biosensors and stimulus molecules in each region is associated with a threshold for a production of an output molecule or an alteration of an output property of the output molecule, the biosensors, or the stimulus molecules, wherein the production or the alteration is based on a value associated with an information source, detecting an output received from one or more of the multiple regions corresponding to the output molecule or the output property in that region, and generating an electric signal associated therewith, and processing the electrical signal to determine the value associated with the information source.

Disclosed herein are compositions and methods for activating a light responsive modified protein kinase R-like endoplasmic reticulum kinase (PERK) and the Integrated Stress Response (ISR) pathway in a cell.

The invention belongs to the field of biomedicine, and discloses a method and a kit for monitoring a lysosome acidification process mediated by mitochondrial-lysosome interaction. According to the method, an LYSO-PZ probe with a specific structure is used for marking cell lysosome contents, a pH rodo dye and a lysosome membrane marker sfGFP-LAMP1-mCherry are combined for monitoring the pH value and membrane positioning of the lysosome respectively, then a three-channel fluorescence image is obtained through a structural illumination microscope (SIM), and the fluorescence intensity ratio is quantitatively analyzed. In addition, the method further comprises a step of forming mitochondrial-lysosome contact (MLC) through optogenetics regulation, related protein binding is induced through blue light, fluorescence intensity changes before and after MLC formation are compared, and MLC-mediated proton transport is verified. A complete monitoring system is established for the first time, lysosome acidification can be accurately monitored, information can be obtained in multiple dimensions, and the method has important clinical application value and wide market prospects.

The application belongs to the field of biomedicine, and discloses a method and a kit for monitoring a process of mitochondrion-lysosome interaction-mediated lysosome acidification. The method is characterized in that a cell lysosome content is first labeled with a LYSO-PZ probe with a specific structure, a pHrodo dye and a lysosome membrane marker sfGFP-LAMP1-mCherry are combined to monitor the pH value and membrane localization of the lysosome, respectively, then three-channel fluorescence images are acquired through a structured illumination microscope (SIM), and the fluorescence intensity ratio is quantitatively analyzed. In addition, the method further comprises a step of optogenetic regulation of mitochondrion-lysosome contact (MLC) formation, the fluorescence intensity change before and after the formation of MLC is compared through blue light induction of related protein binding, and the proton transport mediated by MLC is verified. The application first establishes a complete monitoring system, can accurately monitor lysosome acidification, and has important clinical application value and broad market prospect.

A surgical catheter including a first end, a second end, a main tube connecting the first end to the second end, a fluid delivery tube connected to the main tube, a fiber optic cable extending from the second end through the main tube, a button positioned near the fluid delivery tube and a needle base unit in the first end having a needle extending through the needle base unit into the main tube.

The invention discloses an optogenetics system for promoting targeted homing of MSC (mesenchymal stem cells) to damaged liver and a preparation method of the optogenetics system, and develops light-controlled CXCR4 release plasmids containing Uvr8 and Cxcr4 sequences and up-conversion nanoparticles capable of converting NIR (non-infrared radiation) with strong tissue penetrating power into UVB on the basis of the characteristics that UVR8 presents a dimer structure in a natural state and is dissociated after being irradiated by UVB (ultraviolet B). The method is used for realizing overexpression of CXCR4 only on an MSC cell membrane in a liver region so as to promote the targeted homing effect, and can effectively overcome the defects of high carcinogenic risk, complicated operation, low cost benefit and the like in the existing method.

Exemplary systems, method and compute-accessible medium can be provided, e.g., for a visual stimulation or an optogenetic activation during a scan of at an object. For example, it is possible to utilize a controller which can be configured to receive signal information regarding the scan, and to precisely control a light engine. The exemplary control of the light engine can be used to activate and deactivate a light source or a plurality of light sources (e.g., one or more at least one light emitting diodes). using the signal information. For example, a digital micromirror device can be utilized through which the light engine is precisely controlled for a full field projection, or patterned projection, wherein the light engine can comprise up to eight high power light sources.

Provided are sequence variants and fusion proteins of the photosensitive ion channel protein ChRmine and their use in treating retinal neurodegenerative diseases. These ChRmine variants and fusion proteins exhibit improved protein properties and enhanced photoresponsive characteristics compared with the wildtype ChRmine, therefore may provide effective optogenetic tools to improve the therapeutic treatment of the retinal neurodegenerative diseases.

The invention discloses an iridium complex as well as a preparation method and application thereof, and relates to the technical field of optogenetics. The iridium complex has the following structural formula, X is an anion, Y is selected from an alkane derivative, an ether derivative or an amine derivative, the iridium complex provided by the invention is regular in structure, the central metal atom iridium has excellent fluorescent property, and fluorine atoms and trifluoromethyl groups introduced into the ligand have excellent fluorescent property, so that the fluorescence intensity of the iridium complex is greatly improved. The electron structure and the energy level of the complex are obviously regulated, the photophysical performance of the complex is enhanced, and excellent two-photon absorption capacity is shown, so that the complex has huge application potential in the fields of biological imaging, photodynamic therapy and the like.

The present invention relates to a two-photon holographic optogenetic stimulation method, system, device, and medium based on wavefront correction. The method comprises: step S1, selecting a target neuron from two-photon imaging and obtaining a hologram corresponding to the three-dimensional spatial position of the target neuron; step S2, selecting a target region on the hologram requiring wavefront distortion correction for the target neuron, and performing wavefront distortion correction on the target neuron in the target region to obtain a phase map containing wavefront distortion information and position information; and step S3, optically exciting the target neuron based on the phase map containing wavefront distortion information and position information. Compared with existing technologies, the present invention achieves deep single-cell resolution excitation of more neurons with a large field of view, lower excitation power, and significantly improves the accuracy and safety of optogenetic stimulation imaging.

The invention discloses a method for promoting plant growth and increasing yield through optogenetics, and belongs to the technical field of plant growth and development research. By activating light-operated hydrogen ion pump PPR protein, plant growth can be promoted, and the yield can be increased. By transferring the light-operated hydrogen ion pump PPR protein, the plant can grow quickly under the same growth condition. The growth promotion mechanism does not depend on frequent stimulation of external conditions, but accelerates a series of key physiological activities, such as a photosynthetic phosphorylation process, substance transmembrane transport and the like, regulated by hydrogen ions in plants by means of illumination, so that the plant growth efficiency is remarkably improved. And the growth target of the plant can be realized in a shorter time.

Provided herein are AAV2 vectors encoding SEQ ID NO:4 as optogenetic proteins and methods of use, for example, for retinitis pigmentosa.

The invention provides a flexible miniature LED device and a preparation method thereof, and relates to the field of miniature LEDs. The device comprises an energy supply driving unit which is used for providing an electric signal and sending a control signal; a flexible substrate; the micro LED array is arranged on the surface of the flexible substrate and connected with the energy supply driving unit, and the micro LED array is used for converting the electric signal into an optical signal and controlling the light emitting parameter of the optical signal according to the control signal; wherein the energy supply driving unit is connected with the micro LED array through a flexible wire. According to the invention, the micro LED array is connected with the energy supply driving unit through the flexible wire, so that the energy supply driving unit is utilized to regulate and control the micro LED array. The problems that an existing optogenetics device is difficult to emit controllable photostimulation and is inconvenient to control are solved.

The present invention relates to a forebrain assembloid that exhibits maturity similar to that of the human brain and expresses ChR2 to enable optogenetic analysis and, more specifically, to: a method for producing a forebrain assembloid that enables optogenetic analysis; a forebrain assembloid produced by the method; and a forebrain assembloid having a single rosette structure, comprising six cortical layers and cavities, wherein the cortical layers include glial cells and uniformly express ChR2.

The present invention includes methods and systems for optical assays, such as optogenetic assays, of biological activity in which an optical reference signal is used to normalize an optical test signal.

The invention belongs to the technical field of parameter control, and discloses a purification parameter control system and method for tryptophan in peanut buds. The method comprises the following steps: acquiring frequency shift of a chromatographic column through ultrasonic waves, analyzing to obtain flow velocity, and analyzing to obtain flow field uniformity and vortex intensity according to the flow velocity; an optogenetics probe is constructed, and initial fluorescence intensity is acquired through blue light activation and acquisition; when the uniformity of the flow field is smaller than a uniformity threshold value, the initial fluorescence intensity is compensated through the vortex intensity to obtain the fluorescence intensity; synchronously collecting temperature and ORP potential, and constructing a separation kinetic model; obtaining the enantiomer impurity concentration, and judging the process stage; setting a dual-stage optimization target according to the process stage, performing optimization in combination with the separation kinetic model to obtain an optimization purification parameter, and generating a corresponding control instruction in combination with a control barrier function; through interdisciplinary fused multi-physics field modeling and an intelligent optimization framework, accurate control and green and efficient production of the tryptophan purification process are realized.

A device and a signal processing method that can be used with a device to recognize and distinguish a physiological high-frequency oscillation (HFO) from a pathological high-frequency oscillation. The signal processing method detects a physiological HFO in the electrical brain signal one regimen of electrical or optogenetic brain stimulation can be triggered, alternatively if the method detects a pathological HFO associated with epilepsy a different regimen of electrical or optogenetic brain stimulation can be triggered. Thus, the signal processing method can be utilized in a closed loop brain stimulation device that serves the dual purpose of both enhancing memory encoding, consolidation, and recall, or improving cognition, and reducing the probability of a seizure in a patient with epilepsy.

The invention belongs to the technical field of biomedical engineering and synthetic biology, and particularly discloses a biofilm dynamic regulation and control system and method based on microfluidics and optogenetics, the system comprises a microfluidic chip, a detection module and an optogenetics regulation and control module, the detection module is electrically connected with the microfluidic chip through a multi-model detection interface, and the optogenetics regulation and control module is electrically connected with the detection module through a multi-model detection interface. The detection module comprises a laser confocal microscope, a [mu] CT three-dimensional reconstruction instrument and a DESI-MSI mass spectrum imager; the photogenetic regulation and control module comprises pseudomonas fluorescens, and the genome of the pseudomonas fluorescens is integrated with a red light response type PhyB-PIF3 gene loop. The biofilm dynamic regulation and control system and the biofilm dynamic regulation and control method based on microfluidics and photogenetics are used for accurately regulating and controlling the physicochemical characteristics of the biofilm and quorum sensing (QS) signal molecule expression, and can monitor the thickness of the biofilm, porosity distribution QS signal molecules and the diffusion efficiency of an antibacterial agent in real time and in situ.

Disclosed are a neural probe, a neural probe interface device connected to the neural probe, and a biological experiment system including the neural probe and the neural probe interface device. The neural probe collects a neural signal generated from neurons based on an optogenetics technique. The neural probe includes a neural probe substrate, an optical input port disposed in the neural probe substrate and configured to receive an optical signal from the outside, an optical output port disposed in the neural probe substrate and configured to transmit the optical signal to neurons, a first electrode configured to receive the neural signal, and a second electrode configured to receive the neural signal from the first electrode through an electric wire and to transmit the neural signal to the outside by using any one of a wired communication method and a wireless communication method.

The invention discloses intelligent optimization of soil microbial community and plant synergistic remediation in a phytoremediation experimental facility, and a system monitors soil pollutants, microbial metabolites and plant root exudates in real time, utilizes a microbial sensor and a nano-robot modified by synthetic biology, and an optogenetics technology to realize intelligent optimization of soil microbial community and plant synergistic remediation. Plant-microorganism interaction, a quantum annealing algorithm and a federated learning framework are optimized to generate an optimal repair strategy, the bionic mechanical arm and the laser-nanotechnology execute precise operation, the microbial fuel cell and the piezoelectric energy recovery unit realize self-energy supply of the system, and the system has the characteristics of high efficiency, intelligence and environmental friendliness and is suitable for large-scale popularization and application. The method is suitable for repairing complex soil pollution.

The present disclosure provides an isolated photosensitive opsin protein that rapidly, reversibly and accurately restores the sensitivity of retinal cells to light by activating Gq signaling.

A medical probe for optogenetics, including a flexible substrate made of two-dimensional conductive material, a plurality of vertical-cavity surface-emitting III-V semiconductor microlasers, referred to as elementary lasers, including an active layer disposed between a lower reflective layer and an upper reflective layer, a lower semiconductor contact disposed between the lower reflective layer and the substrate, and a lower metal contact disposed on the substrate and connected to the lower semiconductor contact via the substrate, an upper semiconductor contact disposed on the upper reflective layer, and an upper metal contact connected to the upper semiconductor contact, the lower metal contacts of the elementary lasers being intended to be electrically connected to a common potential, a biocompatible encapsulation layer.

The utility model provides a wireless photogenetic system of a charging version, which solves the problems that the activity range of an animal is limited and the experimental design is influenced in the existing photogenetic experiment through an animal head connection mode, and the main scheme is that the wireless photogenetic system comprises a control host and a plurality of implantable photoelectric probes, the plurality of implantable photoelectric probes are independently packaged and can be independently controlled through the control host, the plurality of implantable photoelectric probes can be charged through the charging box at the same time, each implantable photoelectric probe comprises an RF chip, an antenna, a battery, a flexible probe substrate and a miniature LED, and the front and back surfaces of the flexible probe substrate are provided with a plurality of groups of joints. The micro LEDs are electrically connected with the connectors in a one-to-one correspondence mode, the battery is electrically connected with the RF chip and the micro LEDs, the RF chip is electrically connected with the micro LEDs and transmits external signals through the antenna, and the control host adjusts output of the micro LEDs through the driving circuit.

The present disclosure provides an isolated photosensitive opsin that rapidly, reversibly, and precisely restores light sensitivity to retinal cells by activating Gq signaling.

Disclosed are methods and compositions for real-time monitoring cellular calcium ion dynamics by red-shifted genetically encoded indicators under optogenetic control, in which millisecond-timescale of temporal control of optical activation or inactivation and signal recording can be achieved. The methods include artifact-free functional imaging in conjunction with optogenetic tools for studying cellular physiology, signal transduction and neuronal activity. Thus, all-optical and non-invasive approaches for drug screening, toxicity testing and assessment of cell functions may be provided.

The application discloses a method for precisely regulating wax synthesis of plant leaves based on light-controlled chloride ion channel protein and belongs to the technical field of genetic engineering. The specific process is as follows: the light-controlled chloride ion channel protein opens or closes the ion channel under irradiation of different light, and the extracellular flow of chloride ions is promoted or terminated, so that the effect of promoting or terminating the wax synthesis is achieved. According to the principle of optogenetics, the application realizes the precise, reversible and space-time controllable regulation of the wax synthesis of the plant leaves, and assists in mining more genes related to the wax synthesis of the plants, thereby providing technical support for improving the adaptation ability of the plants to adversity.

The invention discloses a bicolor mu-LED and signal acquisition integrated optogenetics nerve probe and a preparation method thereof, and belongs to the technical field of optogenetics nerve probes. The nerve probe sequentially comprises a flexible substrate, a GaN-based multi-quantum well layer I, a band-pass filter layer, an isolation layer I, a GaN-based multi-quantum well layer II, an isolation layer II, a collection electrode layer and a passivation layer from bottom to top, in the first GaN-based multi-quantum well layer, InGaN and GaN are used for constructing a multi-quantum well layer, and in the second GaN-based multi-quantum well layer, AlGaInP is used for constructing a multi-quantum well layer, so that the mu-LED respectively shows blue light and amber light. Mu-LED excitation and energy modules are arranged at the front end and the rear end of the first GaN-based multi-quantum well layer and the front end and the rear end of the second GaN-based multi-quantum well layer respectively, and Mu-LED excitation sources and the energy modules are the same in material and structure and are connected through metal wires. The energy module receives an external light signal, converts the external light signal into an electric signal, and provides a driving current and an excitation signal for the mu-LED excitation source.