24 results about "Interneuron" patented technology

The invention relates to a neural network global one-time structured pruning method, system and device and a medium, and the method comprises the steps: carrying out the parallel capturing of the input activation tensors of all target layers in a to-be-pruned neural network through a calibration data set through single-time forward propagation; according to an input activation tensor, synchronously calculating a difference entropy index and amplitude response intensity for an intermediate neuron weight group of each target layer, and performing normalization and fusion to form a static global importance map; determining an importance threshold according to a preset pruning rate, and generating a global to-be-pruned index set of which the mixed importance score is lower than the importance threshold at one time based on the global importance map; and on the basis of the index set, performing one-time physical structured pruning on the weight matrix of each target layer. Therefore, the static global importance map is generated through single forward propagation and parallel capture of the activation tensor, and maskless one-time pruning is completed through physical structured pruning.

The present application relates to a kind of neural network global one-time structured pruning method, system, equipment and medium, wherein, method includes: by single forward propagation, in the neural network to be pruned using calibration dataset and parallelly capturing the input activation tensor of all target layers;According to input activation tensor, the intermediate neuron weight of each target layer is synchronously calculated differential entropy index and amplitude response intensity, and after normalization and fusion, static global importance atlas is formed;According to the importance threshold value determined according to preset pruning rate, based on global importance atlas, one-time generates the index set of all global pruning to be pruned whose mixed importance score is below importance threshold value;Based on index set, the weight matrix of each target layer is executed one-time physical structured pruning.By doing so, the present application generates static global importance atlas by single forward propagation and parallelly capturing activation tensor, and completes no-mask one-time pruning by physical structured pruning.

Methods for generating mature somatostatin+ interneurons from human forebrain neural progenitor cells are provided using chemically-defined culture media in a two-stage culture protocol. The mature somatostatin+ interneurons are generated from medial ganglionic eminence neural progenitor cells (MGE-NPCs), which themselves are differentiated from pluripotent stem cells. Culture media, isolated cell populations and kits are also provided.

Provided herein are methods of generating hindbrain cells, including respiratory hindbrain cells, from pluripotent stem cells. Also provided are methods of generating a three-dimensional organoid comprising a population of hindbrain cells including a heterogeneous population of interneurons.

A trivial transmitter model enables processes to be automated and / or networks to be managed efficiently and reliably. The trivial transmitter model includes one or more interneuron units that receive a quantity of transmitters including at least a portion of a first quantity of a source transmitter released by a source unit, determine a second quantity of an interneuron transmitter based on the quantity of received transmitters including at least the portion of the first quantity of the source transmitter, and release the second quantity of the interneuron transmitter, wherein the second quantity of the interneuron transmitter is configured to perform an action upon satisfying a predetermined threshold.

The invention relates to a composition for inducing reprogramming of mesenchymal stem cells and application of the composition, and belongs to the technical field of biological medicine. According to the present invention, it is firstly found that the composition of CHIR99021, Y27632, SB431542 and purmorphamine can induce the reprogramming of mesenchymal stem cells into cholinergic motor neurons and glutamic acid energy interneurons, the cholinergic motor neurons are mainly adopted, and the induction efficiency of the composition is the highest; and inoculating the mesenchymal stem cells onto the silk fibroin scaffold, adding CYSP for induced culture, and finding that the induced neuron-like cells wrap the periphery of silk fibroin fibers to form a transplantable neuron network. When the neural network is transplanted into a spinal cord injury rat model, the deposition of collagen in a spinal cord injury cavity of a rat transplanted with the neural network is reduced, host neurons are saved, nerve fiber regeneration is promoted, and the injured spinal cord is repaired.

Methods for increasing auditory cortex interneuron excitability and improving auditory perception, in particular pitch-discrimination acuity, are provided, which include the use of a Vasoactive Intestinal Peptide Receptor 1 (VIPR1) inhibitor.

Provided are an in vitro preparation method for medial ganglionic eminence (MGE) progenitor cells and GABA interneurons, comprising the following steps: providing human-induced neural precursor cells, inoculating HiNPCs on a culture plate coated with poly-L-ornithine hydrobromide and laminin, and performing differentiation culture using an MGE differentiation culture medium until a cell cluster is formed; transferring the cell cluster and inoculating same into the MGE differentiation culture medium, removing dead cells from a surface of a cell sphere, tilting a culture device, sucking out a part of the culture medium, adding the MGE differentiation culture medium again, and performing culture for 14-15 days to obtain the medial ganglionic eminence progenitor cells; and collecting medial ganglionic eminence progenitor cell spheres and performing the differentiation culture on same again to obtain the GABA interneurons. According to the methods, an induced pluripotent stem cell stage is avoided from the source and the differentiation is confined to the neuroectoderm at the beginning, so that the differentiation direction is more accurate, and the medial ganglionic eminence progenitor cells can be obtained in a relatively short time.

Methods for generating mature somatostatin+ interneurons from human forebrain neural progenitor cells are provided using chemically-defined culture media in a two-stage culture protocol. The mature somatostatin+ interneurons are generated from medial ganglionic eminence neural progenitor cells (MGE-NPCs), which themselves are differentiated from pluripotent stem cells. Culture media, isolated cell populations and kits are also provided.

The invention relates to a combined regulation and control method based on PV neurons, and the method comprises the following steps: building a thalamic cortical neuron group dynamic model, the thalamic cortical neuron group comprises a cortical main neuron, an inhibitory intermediate neuron, a specific relay nucleus and a thalamic reticulate nucleus; constructing a four-state photogenetic channel model, calibrating photogenetic channel parameters, and performing targeted photostimulation on PV neurons; and performing single-parameter scanning and parameter combination optimization on the parameters of the optical genetic channel, and realizing the parameter optimization of the optical genetic channel on the basis of the neuron activity state simulated by the thalamic cortex neuron group dynamic model. Compared with the prior art, the method has the advantages that accurate neuron regulation and control based on a targeted target can be realized, and the like.

This article describes a method for generating mature somatostatin-interneurons from human forebrain neural progenitor cells in a two-stage culture protocol using a chemically defined culture medium. Mature somatostatin-interneurons are generated from medial ganglion ridge neural progenitor cells (MGE-NPC), which themselves are derived from pluripotent stem cells. The article also provides the culture medium, isolated cell populations, and kits.

A protection device for monitoring an energy supply grid has a measured value acquisition device for acquiring measured values which indicate an electrical state of the energy supply grid, and an evaluation device which is connected to the measured value acquisition device and is configured to carry out a plurality of protection functions and, as a result, to make a decision as to whether the energy supply grid is in a permissible or impermissible operating state. Here, the evaluation device be designed entirely in the form of a single neural network with an input neuron layer that receives the measured values and / or values derived therefrom, intermediate neuron layers, and an output neuron layer which outputs a classification of operating states of the energy supply grid. The neural network for classifying the operating states is trained to carry out all protection functions together.

A computer-implemented method of training a neural network to recognize sensory patterns includes obtaining input data, preprocessing the input data in one or more preprocessors of the neural network, and applying the preprocessed input data to a core portion of the neural network. The core portion of the neural network includes a plurality of principal neurons and a plurality of interneurons, and is configured to implement a feedback loop from the interneurons to the principal neurons that supports persistent unsupervised differentiation of multiple learned sensory patterns over time. The method further includes obtaining an output from the core portion, and performing at least one automated action based at least in part on the output obtained from the core portion. The neural network may be adaptively expanded to facilitate the persistent unsupervised differentiation of multiple learned sensory patterns over time by incorporating additional interneurons into at least the core portion.

Compositions and methods for the treatment of brain cancer are disclosed.

The present application relates to a kind of random neuron circuit and its discharge mode extraction method, a kind of neuron circuit is constructed based on threshold transition device, resistance and capacitor, the pulse emission characteristics of neuron is realized based on the circuit.Compared with other neuron circuits based on threshold transition device, the neuron circuit can realize the conversion of different discharge modes under fixed parameters, and due to the randomness of neuron, the mode switching of circuit output has the probability transition behavior, which is beneficial to construct high-order intelligent brain-like system.At the same time, the present application uses the integral and resonance characteristics of H-H neuron itself to extract the mode of the probability output of the previous neuron, uses the resonance characteristics of unimodal detection neuron circuit to extract the unimodal output of intermediate neuron circuit, and uses the integral characteristics of bimodal detection neuron circuit to extract the bimodal output of intermediate neuron circuit, so as to complete the calculation process of multiplexing encoding and decoding of information.

The application belongs to the technical field of animal experiment models, and discloses a method for constructing a mouse model of autism spectrum disorder and application. Pregnant mice are orally exposed to different doses of 1-NP every day during the whole gestation period, the pregnant mice naturally give birth, and the offspring mice are evaluated for autism-like behaviors at PND28 after birth by using a three-chamber social experiment. The offspring mice are evaluated for autism-like behaviors at PND70 after birth by using a three-chamber social experiment. Maternal 1-NP exposure during pregnancy delays the migration of intermediate neurons in the forebrain of fetal mice, and the autism-like behaviors. 1-NP exposure during pregnancy down-regulates the expression of intermediate neuron migration-related genes by reducing DNA hydroxymethylation of the intermediate neuron migration-related genes. 1-NP exposure during pregnancy damages the synthesis of mitochondrial tricarboxylic acid cycle alpha-ketoglutarate (alpha-KG). Maternal alpha-KG supplementation during pregnancy improves the migration of intermediate neurons in 1-NP exposed fetal mice and the autism-like behaviors of offspring mice.

Artificial expression constructs for selectively modulating gene expression in selected central nervous system cell types are described. The artificial expression constructs can be used to selectively express synthetic genes or modify gene expression in GABAergic interneurons.

The invention discloses a social disorder animal model based on a Calretinin positive internals neuron target as well as a construction method and application of the social disorder animal model. According to the invention, an MSEW normal form is applied to construct a social disorder mouse model in a CR-Cre animal, and an action mechanism of Cllresinin positive interneurons in an anterior cingulation cortex region participating in regulation and control of MSEW-induced social disorder is explored. It is found that the Calretinin positive interneurons participate in regulation of MSEW-induced animal social disorders, and a brand new scientific theoretical basis is provided for exploring a mechanism that the Calretinin positive interneurons participate in regulation of the social disorders in the anterior subtraction cortex region.

Artificial expression constructs for selectively modulating gene expression in selected central nervous system cell types are described. The artificial expression constructs can be used to selectively express synthetic genes or modify gene expression in GABAergic interneurons.

Methods for generating mature somatostatin+ interneurons from human forebrain neural progenitor cells are provided using chemically-defined culture media in a two-stage culture protocol. The mature somatostatin+ interneurons are generated from medial ganglionic eminence neural progenitor cells (MGE-NPCs), which themselves are differentiated from pluripotent stem cells. Culture media, isolated cell populations and kits are also provided.

Methods for deriving dorsal interneurons (dIs) of all types, dI1-dI6, and for producing dIs in large quantities without changing their identity, are described. A method for producing dIs in vitro comprises contacting neuromesodermal progenitor cells (NMPs) with specific factors following two variations on a protocol. The methods described herein provide a disease model for sensory processing disorders, such as fibromyalgia, and autism. Cells can be obtained from an individual subject using a skin biopsy to develop the stem cells that can then be used for study and testing that is tailored to the individual subject.

Nucleic acid molecules and compositions, and methods using the same are provided herein for intraocular gene-based delivery and expression of two or more proneural bHLH transcription factors in the retina. The nucleic acid molecules, compositions and methods disclosed herein stimulate regeneration of retinal interneurons from retinal Müller glia (MG) and reprogram the MG into bipolar, amacrine, horizontal, and / or ganglion cells. Such methods and nucleic acid molecules are used for vision restoration and / or treatment of a range of ocular diseases involving retinal degeneration after injury, disease, or vison loss.

A trivial transmitter model enables processes to be automated and / or networks to be managed efficiently and reliably. The trivial transmitter model includes one or more interneuron units that receive a quantity of transmitters including at least a portion of a first quantity of a source transmitter released by a source unit, determine a second quantity of an interneuron transmitter based on the quantity of received transmitters including at least the portion of the first quantity of the source transmitter, and release the second quantity of the interneuron transmitter, wherein the second quantity of the interneuron transmitter is configured to perform an action upon satisfying a predetermined threshold.