261 results about "Precursor cell" patented technology

The invention discloses a serum-free adipogenic differentiation method for porcine adipose precursor cells, and relates to the technical field of mammalian animal cell differentiation.In the adipogenic differentiation process, an induced differentiation culture medium and a maintained differentiation culture medium are sequentially used, and the induced differentiation culture medium is composed of a basic culture medium and cell culture auxiliary factors; the cell culture auxiliary factor is composed of 66nmol / L insulin, 33 [mu] mol / L biotin, 17 [mu] mol / L pantothenic acid, 0.1 [mu] mol / L dexamethasone, 0.25 mmol / L IBMX, 1 [mu] mol / L rosiglitazone, 150 [mu] mol / L sodium oleate and 10 [mu] g / mL transferrin; the maintenance differential medium removes IBMX and rosiglitazone on the basis of inducing the differential medium. The serum-free adipogenic differentiation method for the porcine fat precursor cells, provided by the invention, is good in differentiation effect and high in repeatability.

The invention relates to an organ-like core-shell microsphere as well as a preparation method and application thereof. The preparation method comprises the steps that 1, raw materials are prepared, specifically, shell raw materials and inner core raw materials are prepared, the shell raw materials comprise a photoinitiator and methacrylated hyaluronic acid (HAMA), and the inner core raw materials comprise organoid precursor cell suspension and matrigel; 2) molding: extruding the dispersion phase of the shell raw material wrapping the core raw material to a continuous phase by using a micro-fluidic chip and a high-precision injection pump, cutting the dispersion phase into liquid drops by the continuous phase, and performing illumination curing on the liquid drops to form core-shell microspheres; and (3) incubating, namely culturing the core-shell microspheres in a culture medium, so that the organoid precursor cells in the inner core are developed into organoids. According to the preparation method, rapid forming and curing of the organ-like precursor cells carried by the matrigel are achieved, so that the time of the organ-like precursor cells staying in the oil phase is shortened, the cells can obtain oxygen and nutrient substances, and the cell activity is improved.

The invention provides a cattle FTO gene promoter mutation site and application thereof, and belongs to the field of biotechnology and genetic engineering, the mutation site is located in an FTO gene promoter region on a cattle genome 18 # chromosome, and the mutation site is specifically characterized in that an A base at a transcriptional start site upstream-33 base position is mutated into a G base (chromosome coordinate: Chr: 22040490); according to the mutation, the expression level of the FTO gene is remarkably improved, and the differentiation capacity of intramuscular fat precursor cells is remarkably enhanced; the FTO gene promoter mutation site provided by the invention not only can increase the gene expression quantity, but also can promote the differentiation of intramuscular fat precursor cells, and provides a solid technical basis for the cultivation of new beef cattle varieties and the increase of intramuscular fat content.

The present disclosure relates to cellular compositions that are modified to introduce a nucleic acid or vector expressing the same. Such compositions may be used to deliver nucleic acid to a target cell and treat disease such as cancer.

Provided are a liver precursor cell and a construction method therefor and the use thereof. The method for constructing liver precursor cells comprises the steps of: S0, providing liver precursor cells, a mixed solution and a reprogramming culture medium, wherein the mixed solution comprises a serum-free culture medium, polyethyleneimine and a CLP plasmid; S1, carrying out a transfection treatment on the liver precursor cells and the mixed solution to obtain transfected liver precursor cells; and S2, placing the transfected liver precursor cells into the reprogramming culture medium for subculture to obtain immortalized liver precursor cells. The method solves the problems that in the prior art, liver precursor cells cannot be transformed into immortalized cell strains by means of immortalizing genes and cannot be continuously passaged in vitro.

The invention provides a marker of spinal cord functional neural precursor cells, a detection method and a sorting method, and belongs to the technical field of biological medicine. The invention provides application of a marker detection reagent in preparation of a kit for detecting spinal cord functional neural precursor cells. The invention further provides a sorting-based method for separating the SFNPCs cells, and the cells are sorted through the Marker of the specific SFNPCs, so that the cell purity of the SFNPCs is remarkably improved, the difference between batches is reduced, and a high-purity and homogeneous SFNPCs cell population is obtained. The survival rate of the SFNPCs cells separated and subcultured to the P9 generation by the method disclosed by the invention is more than 85%, and the SFNPCs cells have a good treatment effect on a spinal cord injury model.

The invention belongs to the technical field of biomedicine, and relates to a method for constructing a mouse embryo-derived hypothalamic organ model and application, the invention proposes that a mouse embryo is used for constructing a hypothalamic organ for the first time, and the mouse embryo has a definite development time sequence and genetic operability; the naturally retained vascular endothelial cells and multilineage precursor cells provide unique advantages for constructing a three-dimensional organ containing multiple nucleuses such as a preoptic region, a paraventricular nucleus, an arcuate nucleus and a supraoptic nucleus, and the model not only breaks through the nucleus coverage limitation of the existing iPSCs organ, but also can be used for constructing the three-dimensional organ containing multiple nucleuses such as the preoptic region, the paraventricular nucleus, the arcuate nucleus, the supraoptic nucleus and the like by reconstructing a nerve-blood vessel unit. High-fidelity simulation of complex physiological functions of the hypothalamus is realized, and an irreplaceable technical platform is provided for mechanism analysis of neuroendocrine diseases and high-throughput screening of drugs.

The invention discloses a chicken myoblast and chicken fat precursor cell co-culture synchronous differentiation method and application thereof, and belongs to the technical field of animal cell co-culture and differentiation. The method comprises the following steps: respectively carrying out in-vitro multiplication culture on chicken myoblasts and chicken fat precursor cells, carrying out mixed co-culture, inducing for at most two days by adopting horse serum with the volume percent of 0.5-2%, carrying out pre-differentiation, and continuously carrying out induced differentiation culture for at most one day by using an induced adipogenesis culture medium. According to the synchronous differentiation method for co-culture of the chicken myoblasts and the chicken fat precursor cells, a cell co-culture system is constructed, time sequence induced differentiation is achieved, and a final product with fat droplets distributed in the gaps of muscle bundles (the average distance is 20-50 microns) and capable of recarving natural chicken intermuscular fat textures is obtained. According to the method, the differentiation period is shortened to be within 3 days from 21 days (the efficiency is improved by 600%); the induction cost is reduced by 42.7%; the final product breaks through the sensory acceptance barrier through bionic structure recarving.

Disclosed are in vitro methods for the differentiation of precursor cells into a neural crest cell (NCC) primed to a neurogenic lineage. The methods may include, for example, the steps of activating a Hedgehog signaling pathway (“HH signaling pathway”) in a precursor cell, wherein the precursor cell may be contacted with a neural crest cell induction medium for differentiation of the precursor cell into a neural crest cell. Compositions for carrying out the disclosed methods are also disclosed.

The invention belongs to the field of medicine, and discloses application of POCM-NP-coated A939572 in preparation of a medicine for treating osteoporosis, the POCM-NP-coated A939572 comprises osteoclast precursor cell membrane vesicles and NP-coated A939572 loaded on the osteoclast precursor cell membrane vesicles, and the NP-coated A939572 is PLGA (poly (lactic-co-glycolic acid)) nanoparticles entrapped with the NP-coated A939572. The POCM-NP-coated A939572 obtained by loading the nanoparticles loaded with the A939572 small molecule medicine into the osteoclast precursor cell membrane vesicles has the effect of relieving the osteoporosis, can inhibit osteoclast differentiation and can be used for preventing, treating and relieving the osteoporosis.

The invention relates to the field of cell culture, in particular to a serum-free induced adipogenic differentiation culture medium for fish stem cells and application of the serum-free induced adipogenic differentiation culture medium. The culture medium consists of a basic culture medium and a cell culture auxiliary factor combination, the basic culture medium is one of a DMEM (Dulbecco's Modified Eagle Medium) and a DMEM / F12 culture medium; the cell culture auxiliary factor combination is prepared from oleic acid, soya bean lecithin, vitamin C phosphate, beta cyclodextrin, transferrin, saikoside A, psoralen flavanone methyl ether, dehydroabietic acid and phenyllactic acid. The culture medium suitable for serum-free induced adipogenic differentiation of fish stem cells is obtained by adding a cell culture auxiliary factor combination to replace serum in a traditional adipose precursor cell adipogenic induced differentiation culture medium and dexamethasone IBMX serving as a core component of traditional adipogenic differentiation cocktail, the bottleneck problem in the prior art is solved, and the culture medium is suitable for serum-free induced adipogenic differentiation of fish stem cells. And reliable technical support is also provided for large-scale production of cell culture fish meat.

The invention relates to a new technology for promoting generation of hematopoietic precursor cells from human pluripotent stem cells. Specifically, the present invention relates to a method for preparing hematopoietic precursor cells comprising reducing the expression level of YTHDF2 gene in pluripotent stem cells or reducing the activity of YTHDF2 protein of pluripotent stem cells. On the other hand, the invention also relates to a kit for preparing the hematopoietic precursor cell, and the kit comprises a reagent for reducing the expression level of the YTHDF2 gene or a reagent for reducing the activity of the YTHDF2 protein.

A purpose of the present invention is to provide a method of treatment of a disorder by light stimulation in which light of a specific wavelength such as violet light is irradiated at a specific blinking frequency, and an apparatus used for the same. The present invention treats and / or prevents a disorder by promoting survival, proliferation, and differentiation of oligodendrocyte precursor cells or oligodendrocytes, regenerating or increasing myelin, activating nerves, and activating immunity by irradiating light of a specific wavelength such as violet light to a living body.

Disclosed are compositions, in particular, organoid compositions, derived from more than one donor cell. Further disclosed are methods of making compositions, for example, organoid compositions, that comprise a differentiated cell population derived from more than one donor cell. Donor cells may include, for example, a precursor cell such as an embryonic stem cell or other precursor cell. The disclosed methods use synchronization conditions to produce a synchronized pooled-precursor cell population, which may then be differentiated into an organoid composition. Methods of using the compositions are also disclosed.

Provided are methods of obtaining large scale cultures of muscle-derived muscle precursor cells (MPCs) using microcarriers as growth matrices, methods for obtaining therapeutically effective amounts of these cells, populations of cells obtained by the methods, and compositions comprising expanded cells, and a method for preparing a medicament, for example for the treatment of skeletal muscle dysfunction.

The present invention concerns a method of generating a population of skeletal muscle derived human muscle precursor cells. For this purpose, a specialized FBS-free cell growth medium is used. The invention further concerns a composition comprising such a population of hMPCs for use as a medicament, especially in the treatment of skeletal muscle dysfunction.

The present invention relates to a method of producing cells having the phenotype of cardiac valve cells from precursor cells of valve cells comprising (a) introducing into said precursor cells early growth response 3 (EGR3) and / or a nucleic acid sequence encoding EGR3. The present invention also relates to a valve transplant comprising cells having the phenotype of valve cells obtained or obtainable by the method of the invention. The present invention furthermore relates to cells having the phenotype of valve cells obtained or obtainable by the method of the invention, the valve transplant of the invention or a compound promoting the expression and / or the activity of early growth response 3 (EGR3) for use in treating a subject having a valve defect.

The invention relates to a genetically modified induced neural stem cell and an asynchronous co-culture method. By providing a CRISPR / Cas9 engineered Nurr1-driven dopamine energy report induced neural stem cell model, the differentiation of dopaminergic neural precursor cells and neurons can be monitored in real time, a key pathway related to differentiation heterogeneity is revealed through RNA sequencing, an asynchronous co-culture method is established, and the method has the advantages of simple operation, low cost and high efficiency. The yield and purity of the dopaminergic nerve precursor cells can be improved, and the dopaminergic nerve precursor cells are successfully transplanted and differentiated into dopaminergic neurons in mouse striatum to prove the treatment potential of the dopaminergic nerve precursor cells for Parkinson's disease.

The invention discloses a regulation mechanism research method of depleted precursor CD8T cells, and relates to the field of immunology and molecular biology. Comprising the following steps: detecting the expression level of the ARHGAP9 gene in a chronic virus infection model; constructing a T cell specific ARHGAP9 gene knockout animal model; the influence of ARHGAP9 deletion on the frequency, phenotype and function of the Tpex cell is analyzed; a single cell transcriptome sequencing technology reveals that ARHGAP9 regulates and controls a downstream signal channel of a Tpex cell. By constructing a chronic virus infection model, detecting the expression dynamic state of the ARHGAP9 gene, knocking out an animal model by utilizing T cell specificity ARHGAP9, and combining flow cytometry, qPCR, single cell transcriptome sequencing and other technologies, the invention discloses the effect of the ARHGAP9 as a novel immune checkpoint molecule, and provides a theoretical basis for developing Tpex cell targeting immunotherapy.

The generation of complex organ tissues from human embryonic and pluripotent stem cells (PSCs) remains a major challenge for translational studies. It is shown that PSCs can be directed to differentiate into intestinal tissue in vitro by modulating the combinatorial activities of several signaling pathways in a step-wise fashion, effectively recapitulating in vivo fetal intestinal development. The resulting intestinal “organoids” were three-dimensional structures consisting of a polarized, columnar epithelium surrounded by mesenchyme that included a smooth muscle-like layer. The epithelium was patterned into crypt-like SOX9-positive proliferative zones and villus-like structures with all of the major functional cell types of the intestine. The culture system is used to demonstrate that expression of NEUROG3, a pro-endocrine transcription factor mutated in enteric anendocrinosis is sufficient to promote differentiation towards the enteroendocrine cell lineage. In conclusion, PSC-derived human intestinal tissue should allow for unprecedented studies of human intestinal development, homeostasis and disease.

The invention provides a method for preparing human midbrain dopaminergic nerve precursor cells. The method comprises the step of culturing human pluripotent stem cells in a culture medium with determined chemical components so as to finally obtain the human midbrain dopaminergic nerve precursor cells with expected functions. The invention also relates to functional human midbrain dopaminergic nerve precursor cells obtained by said method, as well as to compositions comprising such cells. The invention further provides application of the midbrain dopaminergic nerve precursor cell and a composition containing the midbrain dopaminergic nerve precursor cell in prevention, modeling and / or treatment of neurological disorders, and particularly, the preparation method of the human midbrain dopaminergic nerve precursor cell can be adapted to be used for preparing the human midbrain dopaminergic nerve precursor cell suitable for clinical application.

The application discloses application of skin precursor cell induced Schwann cell derived vesicles (SKP-SC-EVs) in preparation of a product for treating Parkinson's disease, wherein the SKP-SC-EVs have a protective effect in rotenone or MPP+-induced cell injury, and in a MPTP-induced mouse Parkinson's disease model, the SKP-SC-EVs can be given by a nasal instillation method to improve the motor ability of the mouse and restore the olfactory function, and the product can be used for research and treatment of Parkinson's disease.

Provided are a reagent combination or a kit for constructing an embryoid and the use thereof. The reagent combination or the kit can be used for inducing a stem cell to produce a blastocyst-like lineage precursor cell, a blastocyst-like cell and / or an embryoid, which are based on small-molecule induction and independent of transgenes, and have a single cell source. The produced blastocyst-like lineage precursor cell and blastocyst-like cell have a full blastocyst lineage, and are seed cells having balanced developmental competence and totipotency, which solves the problem of unbalanced cell maturation in the prior art. The produced embryoid is highly similar to natural embryos in terms of morphological characteristics and transcriptomic characteristics, has the capability of highly reproducing post-implantation embryonic development and / or gastrulation, which is beneficial for research on embryonic development in vitro. The method for inducing the stem cell to produce the blastocyst-like lineage precursor cell, the blastocyst-like cell and / or the embryoid by using the reagent combination or the kit is simple, and has high applicability and high embryoid construction efficiency.

To provide a method for evaluating the therapeutic and / or prophylactic action of a skin disease caused by the obstruction and / or inflammation of a pilosebaceous gland system.SOLUTION: A method for evaluating a therapeutic and / or prophylactic action of a substance or agent on a skin disease, the method comprising: bringing the substance or agent into contact with sebocytes or progenitor cells thereof; and evaluating the sebocytes or progenitor cells thereof, wherein the skin disease is a skin disease caused by obstruction and / or inflammation of the pilosebaceous unit.SELECTED DRAWING: None

The invention relates to a method of differentiating pancreatic bi-potent progenitor cells into beta cells comprising cultivating pancreatic endocrine precursor cells in a medium that promotes the cAMP signalling pathway that lies downstream of apical-basal polarity in endocrine precursors, which can be used to increase the differentiation of progenitor cells into beta cells. Furthermore, the present invention relates to a method of enriching and / or isolating one or more cells expressing a marker of apical-basal polarity, thereby isolating beta cells, and to a method of producing a population of beta cells from a population of human embryonic stem cells. Furthermore, the present invention relates to a population of beta cells obtainable by the method, a pharmaceutical composition comprising the beta cells, a cell culture of progenitor cells in a medium comprising a cAMP agonist, a complex comprising a beta cell bound by the binding agents specific for CD133 and CD49a, and a use of specific binding partners for isolating beta cells.

Described herein are methods for making edible fat product using insect cells. The methods include isolating a population of insect cells, which may be precursor cells, seeding the population of insect cells onto a scaffold, and contacting the population of insect cells with a free fatty acid composition or a lipid composition. The cells seeded on the scaffold accumulate lipids, resulting in an edible fat tissue. Further disclosed are a composition of matter made from the method for making edible fat cells, a food product that includes the composition of matter, and an in vitro edible fat tissue comprising a population of insect precursor cells seeded on the scaffold.

The invention discloses a preparation method of a transplantable cell strain for treating Parkinson's disease, which comprises the following steps: injecting pluripotent stem cells into animal body tissues, and inducing through a tissue microenvironment. According to the method, the uniqueness of in-vivo tissues is utilized, so that the stem cells are finally proliferated and differentiated into the nerve-like precursor cells capable of being transplanted for treating the Parkinson's disease, a large number of transplantable cells can be efficiently, conveniently and safely obtained through the method, and the method is used for solving the problem that the source and the number of cells are limited in previous cell transplantation. And a new possibility is provided for treatment of nerve injury diseases.

The invention belongs to the technical field of cell culture, and particularly relates to an umbilical cord blood NK cell culture dish and a use method thereof. The culture dish is reasonable in design, the bone marrow microenvironment is simulated through the three-dimensional micro-scaffold, and the adhesion and proliferation efficiency of cord blood NK precursor cells is remarkably improved by combining biocompatible nanofibers (PLGA) and extracellular matrix proteins (fibronectin and heparin sulfate), so that gt; the amplification multiple is 1 * 10 < 4 >. The dynamic microfluidic gradient channel continuously provides cell factors (such as IL-15 and IL-12) and nutrition, maintains stable glucose concentration (gt, 3mM) and low lactic acid level (lt, 15mM), and prevents metabolic waste from inhibiting amplification. According to the invention, an anti-CD16 antibody and an NKG2D ligand (ULBP2) are fixed on the surface, ADCC effect and tumor targeted killing ability of NK cells are enhanced through a costimulatory signal (CD137L), and cytotoxicity (K562 killing rate) gt is enhanced; 80%.

Disclosed in the present invention are a 3D preparation method for and a use of hepatocytes. In the present invention, hepatic precursor cells are differentiated into functional hepatocytes via low-oxygen conditions and a specific culture medium, and after sampling and counting, hepatic spheroids are subjected to liquid nitrogen cryopreservation. The 3D preparation of hepatocytes does not require a microcarrier, and digestive enzyme digestion is not needed.