359 results about "Precursor cell" patented technology

The invention discloses a serum-free adipogenic differentiation method for porcine adipose precursor cells, and relates to the technical field of mammalian animal cell differentiation.In the adipogenic differentiation process, an induced differentiation culture medium and a maintained differentiation culture medium are sequentially used, and the induced differentiation culture medium is composed of a basic culture medium and cell culture auxiliary factors; the cell culture auxiliary factor is composed of 66nmol / L insulin, 33 [mu] mol / L biotin, 17 [mu] mol / L pantothenic acid, 0.1 [mu] mol / L dexamethasone, 0.25 mmol / L IBMX, 1 [mu] mol / L rosiglitazone, 150 [mu] mol / L sodium oleate and 10 [mu] g / mL transferrin; the maintenance differential medium removes IBMX and rosiglitazone on the basis of inducing the differential medium. The serum-free adipogenic differentiation method for the porcine fat precursor cells, provided by the invention, is good in differentiation effect and high in repeatability.

The invention provides a bone organ model as well as a construction method and application thereof, and the method comprises the following steps: arranging a biological material with a micropore array in a cell culture plate to obtain a bone organ chip; suspending the initial stem cells in a culture medium, and promoting the cells to form a spherical structure through cell aggregation or by using a non-adhesive culture surface, so as to obtain cell spheres; and culturing the cell spheres in the bone organ chip by adopting the culture medium for constructing the bone organ model to obtain the bone organ model. The skeletal organ is successfully constructed through in-vitro self-organized 3D co-culture initial stem cells including embryonic stem cells, induced pluripotent stem cells, bone marrow mesenchymal stem cells, osteoblasts, bone cells, all levels of osteogenic precursor cells, osteogenic-like cells and MC3T3-E1 cells, and the skeletal organ can become an important tool for researching and treating bone related diseases.

The invention relates to an organ-like core-shell microsphere as well as a preparation method and application thereof. The preparation method comprises the steps that 1, raw materials are prepared, specifically, shell raw materials and inner core raw materials are prepared, the shell raw materials comprise a photoinitiator and methacrylated hyaluronic acid (HAMA), and the inner core raw materials comprise organoid precursor cell suspension and matrigel; 2) molding: extruding the dispersion phase of the shell raw material wrapping the core raw material to a continuous phase by using a micro-fluidic chip and a high-precision injection pump, cutting the dispersion phase into liquid drops by the continuous phase, and performing illumination curing on the liquid drops to form core-shell microspheres; and (3) incubating, namely culturing the core-shell microspheres in a culture medium, so that the organoid precursor cells in the inner core are developed into organoids. According to the preparation method, rapid forming and curing of the organ-like precursor cells carried by the matrigel are achieved, so that the time of the organ-like precursor cells staying in the oil phase is shortened, the cells can obtain oxygen and nutrient substances, and the cell activity is improved.

The invention provides a cattle FTO gene promoter mutation site and application thereof, and belongs to the field of biotechnology and genetic engineering, the mutation site is located in an FTO gene promoter region on a cattle genome 18 # chromosome, and the mutation site is specifically characterized in that an A base at a transcriptional start site upstream-33 base position is mutated into a G base (chromosome coordinate: Chr: 22040490); according to the mutation, the expression level of the FTO gene is remarkably improved, and the differentiation capacity of intramuscular fat precursor cells is remarkably enhanced; the FTO gene promoter mutation site provided by the invention not only can increase the gene expression quantity, but also can promote the differentiation of intramuscular fat precursor cells, and provides a solid technical basis for the cultivation of new beef cattle varieties and the increase of intramuscular fat content.

The present invention induces differentiation of mesenchymal stem cells and osteoblast precursor cells into osteoblasts. Provided is a medium for culturing stem cells, the medium including 60-90 vol% basal medium and 10-40 vol% physiological saline, to which 1-100 ng / mL of EGF, 0.2-20.0 ng / mL of FGF-2, 0.2-20.0 ng / mL of PDGF, and 0.5-8.0 mM of magnesium ascorbyl phosphate are further added.

The present disclosure relates to cellular compositions that are modified to introduce a nucleic acid or vector expressing the same. Such compositions may be used to deliver nucleic acid to a target cell and treat disease such as cancer.

Provided are a liver precursor cell and a construction method therefor and the use thereof. The method for constructing liver precursor cells comprises the steps of: S0, providing liver precursor cells, a mixed solution and a reprogramming culture medium, wherein the mixed solution comprises a serum-free culture medium, polyethyleneimine and a CLP plasmid; S1, carrying out a transfection treatment on the liver precursor cells and the mixed solution to obtain transfected liver precursor cells; and S2, placing the transfected liver precursor cells into the reprogramming culture medium for subculture to obtain immortalized liver precursor cells. The method solves the problems that in the prior art, liver precursor cells cannot be transformed into immortalized cell strains by means of immortalizing genes and cannot be continuously passaged in vitro.

Disclosed in the present invention are an immune-privileged nerve cell differentiated from an induced pluripotent stem cell and the use thereof. In the present invention, an induced pluripotent stem cell obtained by means of inactivating the major histocompatibility complex HLA-I and II genes and then overexpressing fusion protein XSG006 constructed from CD47 and CD24 functional domains is differentiated to obtain a separated dopaminergic neural precursor cell with low immunogenicity. The neural precursor cell can escape from T cell attacks, and further escape from NK cell and macrophage killings. The neural precursor cell also effectively escapes from the attack of immune system in vivo. In a Parkinson's disease mouse model and a Parkinson's disease non-human primate (NHP) model, the neural precursor cell can continuously survive and have activity for a long time in a host, and fundamentally reverse Parkinson's disease. The neural precursor cell provides an experimental basis and a theoretical basis for establishing a PD treatment strategy and designing a new stem cell therapeutic drug.

The invention provides a marker of spinal cord functional neural precursor cells, a detection method and a sorting method, and belongs to the technical field of biological medicine. The invention provides application of a marker detection reagent in preparation of a kit for detecting spinal cord functional neural precursor cells. The invention further provides a sorting-based method for separating the SFNPCs cells, and the cells are sorted through the Marker of the specific SFNPCs, so that the cell purity of the SFNPCs is remarkably improved, the difference between batches is reduced, and a high-purity and homogeneous SFNPCs cell population is obtained. The survival rate of the SFNPCs cells separated and subcultured to the P9 generation by the method disclosed by the invention is more than 85%, and the SFNPCs cells have a good treatment effect on a spinal cord injury model.

The invention belongs to the technical field of biomedicine, and relates to a method for constructing a mouse embryo-derived hypothalamic organ model and application, the invention proposes that a mouse embryo is used for constructing a hypothalamic organ for the first time, and the mouse embryo has a definite development time sequence and genetic operability; the naturally retained vascular endothelial cells and multilineage precursor cells provide unique advantages for constructing a three-dimensional organ containing multiple nucleuses such as a preoptic region, a paraventricular nucleus, an arcuate nucleus and a supraoptic nucleus, and the model not only breaks through the nucleus coverage limitation of the existing iPSCs organ, but also can be used for constructing the three-dimensional organ containing multiple nucleuses such as the preoptic region, the paraventricular nucleus, the arcuate nucleus, the supraoptic nucleus and the like by reconstructing a nerve-blood vessel unit. High-fidelity simulation of complex physiological functions of the hypothalamus is realized, and an irreplaceable technical platform is provided for mechanism analysis of neuroendocrine diseases and high-throughput screening of drugs.

The invention discloses a chicken myoblast and chicken fat precursor cell co-culture synchronous differentiation method and application thereof, and belongs to the technical field of animal cell co-culture and differentiation. The method comprises the following steps: respectively carrying out in-vitro multiplication culture on chicken myoblasts and chicken fat precursor cells, carrying out mixed co-culture, inducing for at most two days by adopting horse serum with the volume percent of 0.5-2%, carrying out pre-differentiation, and continuously carrying out induced differentiation culture for at most one day by using an induced adipogenesis culture medium. According to the synchronous differentiation method for co-culture of the chicken myoblasts and the chicken fat precursor cells, a cell co-culture system is constructed, time sequence induced differentiation is achieved, and a final product with fat droplets distributed in the gaps of muscle bundles (the average distance is 20-50 microns) and capable of recarving natural chicken intermuscular fat textures is obtained. According to the method, the differentiation period is shortened to be within 3 days from 21 days (the efficiency is improved by 600%); the induction cost is reduced by 42.7%; the final product breaks through the sensory acceptance barrier through bionic structure recarving.

Disclosed are in vitro methods for the differentiation of precursor cells into a neural crest cell (NCC) primed to a neurogenic lineage. The methods may include, for example, the steps of activating a Hedgehog signaling pathway (“HH signaling pathway”) in a precursor cell, wherein the precursor cell may be contacted with a neural crest cell induction medium for differentiation of the precursor cell into a neural crest cell. Compositions for carrying out the disclosed methods are also disclosed.

The invention belongs to the field of medicine, and discloses application of POCM-NP-coated A939572 in preparation of a medicine for treating osteoporosis, the POCM-NP-coated A939572 comprises osteoclast precursor cell membrane vesicles and NP-coated A939572 loaded on the osteoclast precursor cell membrane vesicles, and the NP-coated A939572 is PLGA (poly (lactic-co-glycolic acid)) nanoparticles entrapped with the NP-coated A939572. The POCM-NP-coated A939572 obtained by loading the nanoparticles loaded with the A939572 small molecule medicine into the osteoclast precursor cell membrane vesicles has the effect of relieving the osteoporosis, can inhibit osteoclast differentiation and can be used for preventing, treating and relieving the osteoporosis.

The invention relates to the field of cell culture, in particular to a serum-free induced adipogenic differentiation culture medium for fish stem cells and application of the serum-free induced adipogenic differentiation culture medium. The culture medium consists of a basic culture medium and a cell culture auxiliary factor combination, the basic culture medium is one of a DMEM (Dulbecco's Modified Eagle Medium) and a DMEM / F12 culture medium; the cell culture auxiliary factor combination is prepared from oleic acid, soya bean lecithin, vitamin C phosphate, beta cyclodextrin, transferrin, saikoside A, psoralen flavanone methyl ether, dehydroabietic acid and phenyllactic acid. The culture medium suitable for serum-free induced adipogenic differentiation of fish stem cells is obtained by adding a cell culture auxiliary factor combination to replace serum in a traditional adipose precursor cell adipogenic induced differentiation culture medium and dexamethasone IBMX serving as a core component of traditional adipogenic differentiation cocktail, the bottleneck problem in the prior art is solved, and the culture medium is suitable for serum-free induced adipogenic differentiation of fish stem cells. And reliable technical support is also provided for large-scale production of cell culture fish meat.

The invention relates to a new technology for promoting generation of hematopoietic precursor cells from human pluripotent stem cells. Specifically, the present invention relates to a method for preparing hematopoietic precursor cells comprising reducing the expression level of YTHDF2 gene in pluripotent stem cells or reducing the activity of YTHDF2 protein of pluripotent stem cells. On the other hand, the invention also relates to a kit for preparing the hematopoietic precursor cell, and the kit comprises a reagent for reducing the expression level of the YTHDF2 gene or a reagent for reducing the activity of the YTHDF2 protein.

A purpose of the present invention is to provide a method of treatment of a disorder by light stimulation in which light of a specific wavelength such as violet light is irradiated at a specific blinking frequency, and an apparatus used for the same. The present invention treats and / or prevents a disorder by promoting survival, proliferation, and differentiation of oligodendrocyte precursor cells or oligodendrocytes, regenerating or increasing myelin, activating nerves, and activating immunity by irradiating light of a specific wavelength such as violet light to a living body.

Disclosed are compositions, in particular, organoid compositions, derived from more than one donor cell. Further disclosed are methods of making compositions, for example, organoid compositions, that comprise a differentiated cell population derived from more than one donor cell. Donor cells may include, for example, a precursor cell such as an embryonic stem cell or other precursor cell. The disclosed methods use synchronization conditions to produce a synchronized pooled-precursor cell population, which may then be differentiated into an organoid composition. Methods of using the compositions are also disclosed.

The use of a polylactic-co-glycolic acid scaffold in promoting cartilage differentiation and regeneration belongs to the technical field of biomedical materials. The present invention includes the use of a polylactic-co-glycolic acid scaffold for non-disease diagnosis and treatment purposes in promoting cartilage differentiation and regeneration. The polylactic-co-glycolic acid copolymer is PLGA (50:50). The polylactic-co-glycolic acid scaffold can promote the differentiation of cartilage precursor cells into cartilage, and increase the formation of cartilage collagen matrix and cartilage proteoglycans. The degradation release liquid of the PLGA (50:50) scaffold material can effectively promote the differentiation of cartilage precursor cells into cartilage, and increase the formation of cartilage collagen matrix and cartilage proteoglycans. The release liquid of the PLGA (50:50) scaffold can be changed into a cartilage microenvironment, thereby playing a role in promoting cartilage regeneration.

Provided are methods of obtaining large scale cultures of muscle-derived muscle precursor cells (MPCs) using microcarriers as growth matrices, methods for obtaining therapeutically effective amounts of these cells, populations of cells obtained by the methods, and compositions comprising expanded cells, and a method for preparing a medicament, for example for the treatment of skeletal muscle dysfunction.

The present invention concerns a method of generating a population of skeletal muscle derived human muscle precursor cells. For this purpose, a specialized FBS-free cell growth medium is used. The invention further concerns a composition comprising such a population of hMPCs for use as a medicament, especially in the treatment of skeletal muscle dysfunction.

Disclosed are method of producing IL-4 exposed M2 macrophage exosomes comprising culturing macrophage or macrophage precursor cells with IL-4 in culture media, and isolating exosomes from the culture media, wherein the isolated exosomes are IL-4 exposed M2 macrophage exosomes enriched with miR-21, miR-99a, miR-146b and miR378a. Disclosed are methods of reprogramming macrophages and / or adipocytes comprising exposing the macrophages and / or adipocytes to IL-4 exposed M2 macrophage exosomes, wherein immune and / or metabolic properties are altered in the macrophages and / or adipocytes. Methods of treating with IL-4 exposed M2 macrophage exosomes.

The invention relates to a method of producing CD34+CD41dim megakaryocyte (MK) progenitor cells, and a substantially pure cell population of megakaryocyte precursor cells obtained by said method and compositions thereof. The invention also relates to a method of producing proplatelet-bearing MKs and / or platelets using the CD34+CD41dim cells.

The present invention relates to a method of producing cells having the phenotype of cardiac valve cells from precursor cells of valve cells comprising (a) introducing into said precursor cells early growth response 3 (EGR3) and / or a nucleic acid sequence encoding EGR3. The present invention also relates to a valve transplant comprising cells having the phenotype of valve cells obtained or obtainable by the method of the invention. The present invention furthermore relates to cells having the phenotype of valve cells obtained or obtainable by the method of the invention, the valve transplant of the invention or a compound promoting the expression and / or the activity of early growth response 3 (EGR3) for use in treating a subject having a valve defect.

The invention relates to a genetically modified induced neural stem cell and an asynchronous co-culture method. By providing a CRISPR / Cas9 engineered Nurr1-driven dopamine energy report induced neural stem cell model, the differentiation of dopaminergic neural precursor cells and neurons can be monitored in real time, a key pathway related to differentiation heterogeneity is revealed through RNA sequencing, an asynchronous co-culture method is established, and the method has the advantages of simple operation, low cost and high efficiency. The yield and purity of the dopaminergic nerve precursor cells can be improved, and the dopaminergic nerve precursor cells are successfully transplanted and differentiated into dopaminergic neurons in mouse striatum to prove the treatment potential of the dopaminergic nerve precursor cells for Parkinson's disease.

The present invention relates to the establishment of an immortalized erythroid precursor cell line capable of conditional maturation, and more specifically, the present invention provides an erythroid precursor cell for producing an erythrocyte having E6 / E7 and Bcl-xL genes transduced therein, and a preparation method therefor.

The invention discloses a regulation mechanism research method of depleted precursor CD8T cells, and relates to the field of immunology and molecular biology. Comprising the following steps: detecting the expression level of the ARHGAP9 gene in a chronic virus infection model; constructing a T cell specific ARHGAP9 gene knockout animal model; the influence of ARHGAP9 deletion on the frequency, phenotype and function of the Tpex cell is analyzed; a single cell transcriptome sequencing technology reveals that ARHGAP9 regulates and controls a downstream signal channel of a Tpex cell. By constructing a chronic virus infection model, detecting the expression dynamic state of the ARHGAP9 gene, knocking out an animal model by utilizing T cell specificity ARHGAP9, and combining flow cytometry, qPCR, single cell transcriptome sequencing and other technologies, the invention discloses the effect of the ARHGAP9 as a novel immune checkpoint molecule, and provides a theoretical basis for developing Tpex cell targeting immunotherapy.

The generation of complex organ tissues from human embryonic and pluripotent stem cells (PSCs) remains a major challenge for translational studies. It is shown that PSCs can be directed to differentiate into intestinal tissue in vitro by modulating the combinatorial activities of several signaling pathways in a step-wise fashion, effectively recapitulating in vivo fetal intestinal development. The resulting intestinal “organoids” were three-dimensional structures consisting of a polarized, columnar epithelium surrounded by mesenchyme that included a smooth muscle-like layer. The epithelium was patterned into crypt-like SOX9-positive proliferative zones and villus-like structures with all of the major functional cell types of the intestine. The culture system is used to demonstrate that expression of NEUROG3, a pro-endocrine transcription factor mutated in enteric anendocrinosis is sufficient to promote differentiation towards the enteroendocrine cell lineage. In conclusion, PSC-derived human intestinal tissue should allow for unprecedented studies of human intestinal development, homeostasis and disease.

The present invention belongs to the technical field of in vitro cell differentiation. The present invention provides a method, composition and application for separation, identification and adipogenic differentiation of mammalian adipocytes. The mechanical method combined with collagenase digestion method is used to successfully separate and obtain mammalian adipocytes. Immunofluorescence technology is used to identify the obtained adipocytes. The adipogenic differentiation method of adipocytes is explored by a combination of multiple adipogenic induction factors. The differentiated mature adipocytes are identified by Oil Red O staining, BODIPY staining, triglyceride (TAG) content determination and / or Western blot technology. The results show that the adipogenic differentiation method of the present invention has good beneficial effects, is an ideal method for studying adipocyte differentiation, and has important application value in the fields of animal husbandry, biomedicine and biological meat cultivation.

The invention discloses a method for enhancing the immune function of bone marrow precursor cells by using a 33-site monoubiquitin molecule, and relates to the field of genetic engineering technology. Bone marrow precursor cells BMPCs (Bone Marrow Precursor Cells) are isolated from mouse bone marrow, and the bone marrow precursor cells are pretreated with a 33-site monoubiquitin molecule, and then adoptively immunized in vitro, and finally subjected to enzyme-linked immunosorbent assay and flow cytometry experiments. The experiment shows that the immune function of the treated bone marrow precursor cells is significantly enhanced, and their antigen-specific CTL induction ability is also enhanced, and their ability to kill tumor cells is enhanced; the 33-site monoubiquitin molecule can increase the ability of bone marrow precursor cells to transfer intracellular lymphocytes to produce perforin and granzyme B. Bone marrow precursor cells enhanced by the 33-site monoubiquitin molecule can be used in the preparation of tumor immunotherapy drugs.

The invention provides a method for preparing human midbrain dopaminergic nerve precursor cells. The method comprises the step of culturing human pluripotent stem cells in a culture medium with determined chemical components so as to finally obtain the human midbrain dopaminergic nerve precursor cells with expected functions. The invention also relates to functional human midbrain dopaminergic nerve precursor cells obtained by said method, as well as to compositions comprising such cells. The invention further provides application of the midbrain dopaminergic nerve precursor cell and a composition containing the midbrain dopaminergic nerve precursor cell in prevention, modeling and / or treatment of neurological disorders, and particularly, the preparation method of the human midbrain dopaminergic nerve precursor cell can be adapted to be used for preparing the human midbrain dopaminergic nerve precursor cell suitable for clinical application.