Growth of neural precursor cells using umbilical cord blood serum and a process for the preparation thereof for therapeutic purposes

Abstract

This invention is concerned with stem cells derived from umbilical cord blood serum and a method for growing human embryonic stem cells and adult cells comprising sera separated from clotted umbilical cord blood, including growing and differentiating cord blood stem cells into neural precursors, comprising transdifferentiating CD34+ stem cells from mononuclear cells derived from umbilical cord blood to neural precursors. The stem cells obtained from the umbilical cord include pluripotent stem and progenitor cell population of mononuclear cells, and separating pluripotent stem and progenitor cell population of mononuclear cells obtained from the umbilical cord blood. A magnetic cell separator is used to separate out cells which contain a CD marker and then expanding the cells in a growth medium containing retinoic acid and one or more growth factors BDNF, GDNF, NGF and FGF as a differentiating agent. The invention is also concerned with the transplantation and repair of nerve damage, stokes, spinal injury, Parkinson's and Alzheimer's, prepared in accordance with the aforesaid method and a media for culturing umbilical cord blood stem calls consisting essentially of cord blood stem cells derived from umbilical cord blood serum.

Description

[0001] The present invention provides the use of sera separated from the clotted umbilical cord blood for growing human embryonic stem cells and adult cells such as Neural precursor cells, for therapeutic purposes in regenerative medicine. In particular, the present invention relates to transdifferentiation of CD+ 34 stem cells from Mononuclear cells derived from Umbilical Cord Blood to Neural precursors and such cells may be used in transplantation and repair of nerve damage, stroke, spinal injury, Parkinson's and Alzheimer's.[0002] Stem cell technology is an emerging field that may yield many promising therapies. Stem cells are special cells that have the ability to develop into many different types of tissue: bone, muscle, nerve, etc. In theory, they could be grown into replacements for almost any part of the human body. Stem cells are typically found in the embryo and umbilical cord of an organism, and in reservoirs within the human body. Researchers hope that stem cells will pr...

AI Technical Summary

Benefits of technology

0023] As stated, Human umbilical cord blood is a fetal product and a waste product during childbirth. During the gestation of the child in the mother's womb, the placenta and the blood present in the placenta nourish the developing fetus and are therefore rich in several growth promoting factors. The invent...

Problems solved by technology

In recent years although significant advances have been made in bone marrow transplantation (BMT), the basic problem of finding a suitable matching donor still remains.

Further bone marrow harvesting is a painful and invasive procedure and many donors are unwilling to donate marrow.

However, the collection procedure is invasive, time-consuming, requires an anaesthetic and is painful for the donor.

However, these conventional culture media are associated with shortcomings and risks.

The pathogens present in FBS are difficult to screen for likely causative agents of diseases in humans.

Hence, using such cells in a human can be life threatening as there is every chance of a pathogen getting transmitted along with these cells.

Human adult blood serum also supports growth of several cells, however, it cannot substitute for FBS, since it does not provide growth factors, present in FBS.

However, the success is limited and it has been shown conclusively that 2% v / v of the tissue culture media should be made up of FBS for optimal growth of the cells.

However, no work has been done in using umbilical cord blood as a source for growing and differentiating stem cells in neural precursors.

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