706 results about "Blood serum" patented technology

The application discloses a construction method and application of an ovary cell line of Quasipaa spinosa. The method comprises the following steps: selecting metamorphosis period larva ovary tissue, disinfecting and obtaining the ovary tissue, and then using collagenase I digestion solution to digest the ovary tissue at 26 DEG C for 1 hour; after the tissue block adheres to the wall, using a low-osmotic-pressure complete culture medium to carry out primary culture at 25-27 DEG C, wherein the culture medium is 60% diluted DMEM / F12 culture medium containing 10%-15% fetal bovine serum; when the cell confluence degree is higher than 70%, using trypsin-EDTA to digest the cells for 1-2 minutes to subculture, and the subculture culture medium contains 30% cell conditional culture medium; and finally, the cells are cryopreserved through programmed cooling. The ovary epithelial cell line of Quasipaa spinosa is successfully established for the first time, the survival rate of the cells after recovery is high, and the karyotype is stable. The constructed cell line can be used for amphibian reproductive biology research, compound screening and in-vitro conservation of germplasm resources.

This invention provides a data storage method and system for tuberculosis detection, relating to the field of tuberculosis detection technology. The data storage method for tuberculosis detection includes the following steps: S1. Collecting whole-genome sequencing data of Mycobacterium tuberculosis, host serum IgG titer, and drug sensitivity test results; S2. Calculating genetic distance D based on a reverse evolution model to generate four-dimensional spatiotemporal coordinates (t, x, y); S3. Performing data partitioning and storage based on the drug target barrier value β; S4. Generating dynamic metadata using a host-pathogen dynamics model and compressing and storing it. This invention implements a dynamic storage entropy adjustment algorithm at the hardware and software collaborative level, continuously optimizing the matching efficiency of data compression and physical storage. This results in an intelligent data hub that can perceive the evolutionary pulse of pathogens and autonomously optimize resources, providing support for clinical tuberculosis prevention and control decisions with temporal depth, spatial correlation, and risk evolution.

The application provides a biological analysis method capable of accurately, simply and quickly detecting the concentration of adenosylcobalamin in human serum. The method is a liquid chromatography tandem mass spectrometry method, octadecylsilane bonded silica gel is used as the column filler, the liquid phase conditions, mass spectrometry parameters and sample processing method are designed and optimized, and the application first proposes and discloses a biological analysis method capable of accurately and quickly detecting the concentration of adenosylcobalamin in human serum: after simple treatment (need to avoid light) such as protein precipitation and nitrogen blowing dry, the sample can be injected, the analysis time is only 4.00 min, the lower limit of quantification is as low as 5 pg / mL, and the sample dosage is only 100 muL. The application can quickly monitor the change of vitamin B12 in the body, meet the requirements of adenosylcobalamin in vivo drug monitoring, pharmacokinetics and bioequivalence, and can effectively improve the safety and reliability of the drug.

This invention discloses a traditional Chinese medicine composition for treating male infertility due to liver stagnation and kidney deficiency, its preparation method, and its application, belonging to the fields of traditional Chinese medicine pharmaceutical technology and andrological reproductive health technology. The composition is formulated with Bupleurum chinense, Tribulus terrestris, Cynomorium songaricum, Cuscuta chinensis, Cornus officinalis, and Epimedium brevicornu in specific weight proportions, following the principles of soothing the liver and relieving stagnation, tonifying the kidney and replenishing essence, and unblocking the meridians to treat impotence. This invention employs an alcohol-water dual-phase fractional extraction process, and can be prepared into various dosage forms such as granules, capsules, and tablets. Pharmacological experiments have confirmed that the composition of this invention can increase sperm density and progressively motile sperm rate, improve penile tissue nitric oxide synthase (NOS) activity, regulate reproductive hormones, and reduce testicular oxidative damage; serum pharmacochemistry and metabolomics have clarified the active components entering the bloodstream and their pathways of action. This invention features a refined formulation, abundant medicinal resources, and simple preparation, making it suitable for large-scale production and clinical application in treating male infertility due to liver stagnation and kidney deficiency.

The application relates to a centrifugal device and method for detecting animal serum samples and relates to the technical field of serum sample detection. The device comprises a body, a spline is rotationally connected to the bottom of the body cavity through a motor, a bracket is connected to the upper end of the spline in a concave-convex abutting mode, an electrically conductive slip ring is arranged on the outside of the motor output end of the bottom of the body cavity, and a stator on the outside of the electrically conductive slip ring is fixed in the cavity of the body through a connecting rod. Through cooperation of a clamping assembly, a sleeve, a clamping seat and a tube clamp, the clamping seat is driven to rotate by rotating the rotating sleeve, the tube clamp is opened and closed in an interlaced mode through meshing of the connecting plate and the gear, various sizes of serum test tubes can be clamped, special parts need not be replaced, work efficiency is improved, meanwhile, when the number of test tubes is odd, the gravity sensor senses the weight, the electric push rod pushes the connecting frame on the symmetrical position to adjust the position, the gravity center is adjusted through cooperation of the rotating structure, the bracket is balanced, sample spilling and pollution are avoided.

This invention discloses a rapid mass spectrometry method and kit for the simultaneous detection of multiple components in body fluids, belonging to the field of biological detection and identification technology. It can rapidly detect trace compounds such as drugs and biomarkers in complex biological samples. This kit mainly utilizes loaded functional materials to achieve extraction and enrichment of target analytes and is equipped with a quick connector for coupling with a detector to achieve rapid detection. Compared with traditional methods, the significant advantages of the method established by this kit are that it does not require complex sample pretreatment, allows for micro-quantitative sampling, requires small sample volumes, facilitates convenient storage of biological samples, is easy to operate, does not require specialized technical personnel, and enables rapid on-site detection when coupled with portable mass spectrometry. This kit has been successfully used for the detection of quinine, procainamide hydrochloride, atenolol, and nortriptyline in complex samples such as blood and serum, and has significant clinical and economic value.

The main objective of this invention is to provide a novel manufacturing method capable of efficiently and directly converting or inducing somatic cells into insulin-producing cells. As an example, a method for manufacturing insulin-producing cells is provided, which involves directly differentiating and inducing insulin production from somatic cells. This method includes a step of culturing somatic cells in a serum-free differentiation-induction medium; or a step of culturing somatic cells in a differentiation-induction medium containing 5 μg / mL or more of insulin. According to this invention, insulin-producing cells with high insulin secretion capacity can be efficiently and directly manufactured from somatic cells. The insulin-producing cells obtained by this invention are useful in regenerative medicine and the like.

The present invention relates to MSLN-specific binding constructs comprising a designed ankyrin repeat domain with binding specificity for MSLN and a chelator capable of bonding to a radionuclide, as well as to such MSLN-specific binding constructs comprising a half-life extending moiety with binding specificity for serum albumin. The invention further relates to methods of producing such radio-labelled MSLN-specific binding constructs, pharmaceutical compositions comprising such constructs, and the use of such constructs or pharmaceutical compositions in methods for treating, imaging or diagnosing diseases, such as cancer.

This article provides methods for treating progressive familial intrahepatic cholestasis of blood (PFIC), including, for example, PFIC2 (bial salt export pump (BSEP) deficiency), using ileal bile acid transport (IBAT) inhibitors (such as ozexibart or a pharmaceutically acceptable salt thereof). These methods may include identifying or selecting subjects with a higher percentage of unconjugated serum bile acids relative to a reference level and administering an IBAT inhibitor to the identified or selected subject.

The invention relates to 4 serum peptide patterns for diagnostics of intracranial hemorrhagic stroke (ICH) in humans and differential diagnosis from acute ischemic stroke that are defined using a list of the 34 quantitative peptides that in sera of patients with ICH are increased 2 folds or more as compared with individuals without stroke and patients with acute ischemic stroke and a list of 16 qualitative peptides detectable in sera of the patients with ICH but not detectable in the sera of individuals without stroke and in sera of patients with acute ischemic stroke.

The present application relates to a kind of detection system and detection method based on microfluidic chip.The detection system based on microfluidic chip includes microfluidic chip for blood detection, centrifugal processing device, chip fluorescence scanning device.The present application also provides the detection method based on the above system.The detection system and detection method provided by the present application have small sample requirement, and the existing blood detection method generally needs 2mL serum (about 4-5mL whole blood) when detecting, the microfluidic chip used in the present application generally only needs 0.3mL whole blood (corresponding to 60 detections).At the same time, with the aid of centrifugal processing device, chip fluorescence scanning device, high-throughput, high-efficiency operation can be realized.

The application discloses a new use of Leonurine or a pharmaceutically acceptable salt thereof in the preparation of a medicament for treating radiation-induced brain injury, specifically, providing a safe and effective therapeutic drug for iron death, neuroinflammation, neuron damage and lipid metabolism disorder accompanying the radiation-induced brain injury, solving the technical problem of lack of specific treatment means in the prior art, and having a wide clinical application prospect. Experiments adopt a C57BL / c mouse 30 Gy whole brain X-ray radiation model, and results show that Leonurine can significantly improve the body weight decrease and survival rate of the model mice, reduce the serum IL-6 and IFN-alpha levels, inhibit the activation of microglial cells in the cerebral cortex and hippocampus, protect and repair damaged neurons, and improve the ultrastructure of mitochondria and synapses; meanwhile, the expression of GPX4 and SLC7A11 proteins in the brain tissue is up-regulated, the GSH content is increased to inhibit iron death, and the levels of GPE, GPS and GPC related to lipid metabolism are restored.

This invention discloses a low-astringency prickly pear lactic acid bacteria fermented beverage and its application in lowering blood lipids and antioxidation, relating to the fields of microbial application and food fermentation technology. The fermented beverage of this invention uses prickly pear juice as raw material and is prepared by fermentation with *Lactobacillus plantarum* R-21. It can significantly reduce the tannin content of prickly pear juice, improve taste, and increase the polysaccharide content and antioxidant properties of the beverage. In vivo experiments show that it can reduce the levels of triglycerides, total cholesterol, and low-density lipoprotein cholesterol in mouse liver and serum, inhibit fat accumulation and fatty degeneration in liver cells, and enhance the activity of antioxidant enzymes in the liver, demonstrating important application value in lowering blood lipids and antioxidation.

The application relates to an electrochemical sensor based on antibody-aptamer synergic recognition and a construction method and application thereof, and belongs to the technical field of electrochemical analysis and biomarker AZGP1 detection technology. The sensor has strong resistance to high-concentration endogenous interference, and has good selectivity when interference and AZGP1 exist. Compared with the prior art, the sensor has the advantages of high sensitivity, high selectivity, simple operation, rapid detection, low cost and the like, and successfully realizes the detection of AZGP1 in a clinical patient serum sample, and provides a new and efficient method for clinical detection of biological recognition molecules.

The application provides a full-passive microfluidic whole blood sample preparation device, which comprises, from bottom to top, a bottom support layer, a bottom hydrophilic layer, a bottom adhesive layer, a middle main body layer, a liquid outlet channel layer, an upper surface hydrophilic layer of the liquid outlet channel, a first intermediate adhesive layer, an upper layer main body layer, a second intermediate adhesive layer, a top cover layer and a product collection and storage cavity sealing patch which are laminated and assembled in sequence. The application does not need any external driving force or active surface treatment, and only relies on the material properties and ingenious structure design, so that the plasma / serum can be automatically and efficiently separated from trace whole blood, and the application has the advantages of simple structure, low cost, convenient operation, one-time use, instant detection application and the like.

The present application relates to the application of Bifidobacterium longum subsp.longum BL21 in the preparation of a preparation for improving hematopoietic dysfunction, wherein the Bifidobacterium longum subsp.longum BL21 is a Bifidobacterium longum subsp.longum BL21 strain with a preservation number of CGMCC No.10452.The present application develops a new microbial strategy for improving hematopoietic dysfunction, and uses the Bifidobacterium longum subsp.longum BL21 in the preparation of a preparation for improving hematopoietic dysfunction.It is found that the Bifidobacterium longum subsp.longum BL21 can increase the number of peripheral blood cells (red blood cells and white blood cells), the content of platelets or the content of hemoglobin, reverse the weight loss caused by hematopoietic dysfunction, and improve the serum inflammatory response and the spleen inflammatory response caused by hematopoietic dysfunction.The present application also provides a new idea for the wide application of the Bifidobacterium longum subsp.longum BL21.

This disclosure relates to a sample diluent and its preparation method. Specifically, this disclosure relates to a sample diluent composed of Tris, Tween-20, BSA, mixed serum, and PC300. A key improvement is that the mixed serum is prepared by mixing goat serum and fetal bovine serum at a volume ratio of 2:1, and the mixed serum accounts for 40% of the sample diluent. The sample diluent of this disclosure is designed for the detection of lambda and kappa type free light chains, significantly reducing sample matrix interference, improving detection specificity and stability, and solving the technical problem of disproportionate dilution after high-value sample dilution. It is suitable for clinical testing, biomedical research, and other scenarios.

FIELD: pharmacology.SUBSTANCE invention can be used to determine the concentration of an anti-tuberculosis drug in blood serum. The method for determining the concentration of bedaquiline in the blood serum in patients with tuberculosis or mycobacteriosis is that whole blood samples are centrifuged at 3000 rpm for 20 minutes, then the blood plasma is collected in sterile 1.5 mL Eppendorf tubes, then 900 mcL of acetonitrile are added to 300 mcL of plasma and centrifuged at 13500g for 15 minutes, then 1 mL of the supernatant is transferred to a chromatographic vial and make the assay by UHPLC MS / MS using an Athena UHPLC C18, 1.8 mcM, 120A, 2.1×100 mm chromatography column, when using an aqueous solution containing 5 g / L of ammonium acetate, 25 ml / L of concentrated acetic acid, 2 ml / L trifluoroacetic acid as mobile phase A and 100% acetonitrile as mobile phase B, the concentration of bedaquiline is determined using a pre-plotted calibration curve.EFFECT: determination of bedaquiline in human blood plasma in a time not exceeding 6 minutes.1 cl, 9 dwg, 6 tbl

This invention relates to a method for constructing a mouse orthotopic model of highly aggregated glioblastoma, belonging to the fields of biomedicine and experimental animal model technology. Glioblastoma cells are resuspended in a composite carrier of a specific ratio of matrix gel and serum-free culture medium, and a low-temperature injection technique is used. Utilizing the thermosensitivity of matrix gel in the mouse intracranial environment (i.e., its phase transition from liquid to gel at a certain temperature), precise colonization and physical locking of tumor cells at target coordinate points are achieved. This method improves upon existing glioblastoma mouse orthotopic model construction techniques that suffer from tumor cell diffusion and loss, low tumor formation rate, irregular tumor morphology, large intragroup variability, and needle reflux due to low viscosity of the inoculation carrier. It provides a good disease model for subsequent screening or evaluation of anti-glioblastoma drugs.

The application belongs to the technical field of medicines, and discloses a mannose-modified nano silver cluster, a preparation method and application of the mannose-modified nano silver cluster as a probe, wherein the mannose-modified nano silver cluster is obtained by coupling bovine serum albumin-nano silver clusters and modified mannose through acid-amine condensation reaction, 14 modified mannose molecules are coupled on each bovine serum albumin molecule, the mannose-modified nano silver cluster can be applied as a probe in biological recognition and target labeling, a new visual tool is provided for receptor-ligand interaction research and drug delivery monitoring, and the application development of the sugar probe in biomedical detection and diagnosis and treatment is promoted.

The present disclosure relates to methods, compositions, and systems for detecting whether a subject exposed to a coronavirus has developed a neutralizing antibody response. Also disclosed are methods for determining whether a patient infected by a coronavirus is likely to respond to treatment with an antibody preparation. Also disclosed are methods for detecting the level of neutralizing antibody response in a sample of serum from a subject exposed to a coronavirus or to a coronavirus vaccine.

The application relates to the field of biological medicine, and particularly discloses application of saliva TP17, TP47 and TP15 specific antibodies in preparation of an auxiliary diagnostic product for neurosyphilis. Based on specific antibody immunoglobulin G (IgG) of Treponema pallidum in saliva, combined detection of saliva TP17 / IgG, TP47 / IgG and TP15 / IgG outputs a risk value of neurosyphilis of a subject, or a four combined Logistic regression discriminant model is constructed by combining serum TRUST titers, and the subject is stratified into a low-risk or high-risk group based on a preset judgment threshold, so that the comprehensive recognition capability for neurosyphilis is improved. The saliva is used as a detection sample, and the application has the advantages of non-invasiveness, convenience, easy popularization, repeatable collection and the like, and can be used for early screening, auxiliary diagnosis and clinical path management of neurosyphilis.

This invention relates to extracts of traditional Chinese medicine, and particularly to the application of a compound extract with lipid-regulating effects in the preparation of lipid-regulating drugs. Experimental studies of this invention have found that a compound traditional Chinese medicine lipid-regulating formula, administered for 14 weeks, can effectively reduce serum total cholesterol levels in hyperlipidemic mice. Furthermore, the lipid-lowering effects of the compound formula at 7.08 g / kg (high-dose group) and 3.54 g / kg (low-dose group) in hyperlipidemic mice are similar to those of simvastatin. Moreover, the dosage of the lipid-regulating compound formula is lower. This important compound lipid-regulating formula described in this invention can be applied to the prevention and treatment of hyperlipidemia, providing a new option for the prevention and treatment of hyperlipidemia.

The present invention belongs to the technical field of sensorineural hearing. Provided is the use of SPTBN1 as a target in the treatment of GJB2-related sensorineural hearing loss. The use comprises: the construction of a stably transfected cell line, wherein: HEK293T cells are cultured using a DMEM culture medium supplemented with 10% fetal bovine serum and 1% penicillin-streptomycin in a humidified incubator at 37ºC with 95% air and 5% CO2, and when the cells reach 80%-90% confluence, cell passage is performed; IP-MS analysis, wherein: protein complexes are purified using Protein A / G immunoprecipitation magnetic beads, a portion of the extracted proteins is used as input, and then 2 μg of anti-Cx26 antibody is added to the remaining protein extract, same are gently pipetted and mixed, and incubated on a rotating shaker at 4℃ overnight; immunofluorescence observation of the co-localization of Cx26 and SPTBN1, wherein: a stably transfected cell line expressing WT-Cx26 and Mut-Cx26 is constructed; and co-immunoprecipitation (Co-IP) validation, wherein: a stably transfected cell line expressing WT-Cx26 is constructed. The present invention overcomes the limitations of therapeutic methods, such as the relatively low targeting specificity and short therapeutic time windows associated with full-length protein supplementation via gene therapy, thereby providing new insight into GJB2-related hearing loss, and a new target and a new treatment for same.

The application provides a high-activity iPSC exosome and a preparation method and application thereof, relates to the technical field of biological medicine, and the preparation method comprises the following steps: obtaining induced pluripotent stem cells; performing flow cytometry sorting to obtain an iPSC subpopulation; performing suspension expansion culture on the iPSC subpopulation to collect a cell culture solution; sequentially performing clarification filtration, ultrafiltration concentration and CD9 antibody affinity chromatography purification, and then eluting and collecting an eluent containing exosomes. The induced pluripotent stem cells overcome donor dependence and batch difference. The specific screening of a cell subpopulation that is positive for CD105, CD73 and CD90 and maintains pluripotency eliminates heterogeneity and guarantees the high activity and uniform efficacy of the exosomes. The use of microcarriers for serum-free suspension culture breaks through the yield bottleneck and realizes large-scale production. Finally, the high-purity and high-activity exosomes are efficiently extracted through ultrafiltration and CD9 antibody affinity chromatography purification.

This invention relates to a traditional Chinese medicine combination for treating intrahepatic cholestasis, which is made from the following raw materials in parts by weight: Artemisia capillaris and Glycyrrhiza uralensis 5:1 and 3:1. This invention also provides the preparation of the above-mentioned medicine and its use in treating intrahepatic cholestasis. Its advantages are: the traditional Chinese medicine composition of this invention (Artemisia capillaris and Glycyrrhiza uralensis decoction) has a significant ameliorative effect on intrahepatic cholestasis (non-obstructive type) induced by a 1% CA-containing diet in mice. Artemisia capillaris and Glycyrrhiza uralensis decoction can alleviate weight loss in mice and improve liver necrosis; significantly reduce serum ALT and ALP levels in mice; reduce the content of total bile acids in the liver and decrease the proportion of toxic bile acid taurine deoxycholic acid (TDCA) in the bile acid pool; upregulate liver BSEP protein expression to promote bile acid efflux; and downregulate liver NLRP3 protein expression to alleviate liver necrosis.

This invention discloses gold nanoparticle-modified metal oxide framework materials, their preparation methods, and applications, relating to the fields of material preparation and detection technology. The preparation method of the gold nanoparticle-modified metal oxide framework material includes: in-situ reduction of chloroauric acid on the surface of ZIF-67 via a reduction method to obtain an Au / ZIF-67 composite material; and calcination of the Au / ZIF-67 composite material using a programmed temperature rise process to obtain the final product. This invention also provides applications of this material in serum metabolite detection and in screening metabolic biomarkers for esophageal cancer diagnosis. The composite material prepared by this invention possesses a unique hollow porous structure and uniformly distributed gold nanoparticles, providing a large specific surface area for enriching metabolites in serum. It also enhances the ultraviolet absorption and photothermal conversion efficiency of the nanocomposite material, while improving the material's conductivity, thereby significantly improving laser desorption / ionization efficiency and achieving high-sensitivity detection.

The application discloses a hemodialysis sufficiency evaluation method based on sodium balance, and relates to the technical field of hemodialysis, and the method comprises the following steps: obtaining the body weight, dry body weight and serum sodium concentration of a patient before dialysis; calculating a target sodium removal amount Na_target=DeltaW* [Na]s / 0.93, and establishing a quantitative equivalent relationship between the body weight increase and sodium accumulation; obtaining or calculating an actual sodium removal amount (including two ways of convection and diffusion); calculating a sodium removal ratio R_Na=Na_actual / Na_target; and evaluating the dialysis sufficiency according to the R_Na. The application firstly changes the dialysis sufficiency evaluation from 'urea removal' to'sodium and water removal', and directly aims at the main pathological burden of kidney failure patients, i.e. sodium and water retention. The sodium removal ratio R_Na is more directly related to the volume state and cardiovascular prognosis of the patient than the traditional Kt / V index. The method only needs the body weight and serum sodium before and after dialysis to be calculated, and can be integrated into the existing dialysis information system.

The present invention relates to a method of identifying rejuvenating factor(s) by culturing bone marrow cells wherein age-specific human serum is added to the bone marrow cells and to a method of testing active agents to augment the effect of the one or more rejuvenating factor(s) identified.