422 results about "Nicotinamide mononucleotide" patented technology

The invention relates to the technical field of biomarkers, in particular to a blood biomarker combination for prediction and auxiliary diagnosis of ovarian reserve hypofunction (DOR), a corresponding detection kit and application of the blood biomarker combination. According to the invention, the content change of the nicotinamide mononucleotide (NMN) and the metabolite combination thereof in serum or plasma is screened for the first time and can be used as a detection index for predicting the ovarian reserve function decline. Based on the biomarker combination, the invention further provides an in-vitro detection kit containing a related standard substance, an internal standard solution and an extracting solution, and a data analysis method combined with a random forest model, so that an individual with a normal ovarian reserve function and an individual with hypofunction can be accurately distinguished. The method is noninvasive, simple, convenient, high in sensitivity and suitable for early recognition and layered screening of clinical ovarian reserve hypofunction.

The invention provides an NAD < + > composition for skin anti-aging and firming cosmetics and a preparation process of the NAD < + > composition. The NAD + composition is prepared from the following components in parts by weight: 40 to 50 parts of dipropylene glycol, 0.1 to 1 part of nicotinamide, 0.001 to 0.01 part of nicotinamide adenine dinucleotide, 0.05 to 0.1 part of beta-nicotinamide mononucleotide, 0.1 to 0.5 part of leontopodium alpinum flower / leaf extract, 0.1 to 0.5 part of leontopodium alpinum callus extract, 0.1 to 0.5 part of leontopodium alpinum yeast fermentation lysate filtrate and 0.1 to 0.5 part of citric acid. The NAD + composition has the advantages that the preparation process is simple, the prepared NAD + composition has excellent synergy when being compared with some natural plant extract compositions, the effects of resisting aging and tightening skin can be better achieved, the NAD + composition can be effectively absorbed by the skin, the cell regeneration and differentiation capacity is promoted, the resilience force of the skin is helped to be improved, the skin is protected, and the skin is protected. The tightening and anti-aging effects are achieved.

The present invention aims to discover a new function of a composition comprising a combination of nicotinamide mononucleotide (NMN) and a food additive reducing agent derived from shellfish and natural zeolite, and to provide a use based on the new function. [Solution] The cell activator contains nicotinamide mononucleotide (NMN) and a food additive reducing agent obtained by adding baked shell powder obtained by pulverizing baked shells and baked zeolite powder obtained by pulverizing baked natural zeolite to water, subjecting them to a heat treatment in the water, and drying them.

The present invention provides a dressing capable of promoting healing of a chronic wound. Specifically, provided is a multifunctional wound dressing, comprising: first active components: β-nicotinamide mononucleotide (NMN), nicotinamide adenine dinucleotide (NAD+), or a combination thereof; and a dressing matrix. Experiments of the present invention prove that the multifunctional wound dressing of the present invention has an excellent effect of promoting healing of chronic refractory wounds such as diabetic feet.

The invention belongs to the field of nano preparations, and discloses a preparation method of nano microspheres coated with a nicotinamide mononucleotide shell membrane structure, which comprises the following steps: coating NMN in a pregel formed by chitosan and sodium tripolyphosphate, heating and stirring, adjusting the pH value to separate out chitosan-coated nano microspheres, drying to remove moisture on the surfaces of the nano microspheres, and drying to obtain the nano microspheres coated with the nicotinamide mononucleotide shell membrane structure. Then selecting a water phase and an oil phase in a proper proportion, preparing a chitosan / sodium alginate composite nano-microsphere with sodium alginate as an outer membrane by adopting an emulsion cross-linking method, and washing and drying to obtain the NMN nano-microsphere with a core-shell structure. According to the invention, NMN is coated in the nano-microsphere which is composed of two materials and has a nuclear membrane structure, so that the stability of NMN in a natural environment can be improved, hydrolysis of NMN by gastric acid and gastrointestinal tract enzyme in a drug delivery process can be prevented, the bioavailability of NMN is improved, and a drug sustained release effect is achieved.

The present invention relates to a method of increasing nicotinamide mononucleotide (NMN) and / or nicotinamide adenine dinucleotide (NAD +) in the intestinal tract of a subject, the present invention relates to a method for treating a subject by administering to the subject a combination of at least one Bifidobacterium sp. Capable of growing on a sialylated HMO and having a functional de novo nicotinamide adenine dinucleotide (nad operon (nadC, nadD, and nadE) and at least one fucosylated oligosaccharide.

The invention provides anti-aging moisturizing essence and a preparation method thereof, and belongs to the technical field of cosmetics. The essence is prepared from a plant extract, sodium pyrrolidone carboxylate, hexapeptide, nicotinamide mononucleotide, vitamin B5, galactose yeast fermentation product filtrate, b-glucan, sodium hyaluronate, glycerol, tranexamic acid, blue copper peptide, arbutin, nicotinamide, vitrin, a bifidus yeast fermentation product, a bifidus yeast fermentation lysate and the like. Wherein the plant extract is prepared by mixing rosemary, frankincense resin, tea flowers, sandalwood and jasmine flowers through ultrasonic extraction, comprehensive skin care is achieved from multiple angles of anti-aging, whitening, moisturizing and repairing, the overall health of the skin is guaranteed, and the skin care cream is suitable for various skin types.

The invention relates to the technical field of biology, in particular to a recombinant strain for producing nicotinamide mononucleotide and application of the recombinant strain. According to the method, accumulation of the NMN is improved through metabolic flux redirection and gene knockout, a synthesis path of the NMN is successfully established in escherichia coli, and further, the yield of the NMN is effectively improved and reaches 7.0 g / L by optimizing a transport system of NAM-NMN and a PRPP synthesis path. The invention also optimizes the production process of the NMN, realizes the highest yield of the NMN of 28.5 g / L in a fed-batch fermentation system, and obviously improves the yield compared with the traditional batch fermentation.

The invention belongs to the technical field of bioengineering, and particularly relates to a polyphosphate kinase mutant and application thereof in synthesis of beta-nicotinamide mononucleotide, and the polyphosphate kinase mutant is obtained by single-point or multi-point mutation of 99th, 186th, 190th and 204th amino acids of polyphosphatase with an amino acid sequence shown as SEQ ID NO.1. The invention also relates to a preparation method of the polyphosphate kinase mutant, and the application of the polyphosphate kinase mutant in synthesis of beta-nicotinamide mononucleotide in synthesis of beta-nicotinamide mononucleotide in synthesis of beta-nicotinamide mononucleotide in synthesis of beta-nicotinamide mononucleotide. Polyphosphokinase derived from Lampropedia Hyalina DSM 16112 is subjected to molecular modification to obtain multiple mutants, the enzyme activity of the mutants is improved by 1.5-14 times compared with that of wild type polyphosphokinase, the preference of the mutants to AMP is improved by 1.08-4.88 times compared with that of the wild type polyphosphokinase, and an ATP regeneration system constructed by the mutants can remarkably reduce the use amount of enzyme and reduce the fermentation capacity and cost; the reaction time can be greatly shortened, the influence of AMP on the enzyme activity is reduced, the requirements of large-scale industrial production of NMN prepared by adopting a biological enzyme method can be met, and the method has a wide application prospect.

A composition capable of resisting inflammation and promoting skin repair, includes platelet-rich plasma(PRP) and further includes β-nicotinamide mononucleotide(NMN) and sialic acid(NANA). The composition can significantly accelerate wound healing. Compared with separate use of the platelet-rich plasma, the NMN or the sialic acid,the composition promotes faster wound healing. Moreover, after wound scar repair, the resulting scar appears lighter in color.

The invention belongs to the field of biological catalysis, and particularly relates to a method for preparing oxidized nicotinamide adenine dinucleotide phosphate through double-enzyme coupling. According to the invention, through construction and optimization of a one-pot double-enzyme coupled catalytic system, a genetically engineered bacterium containing nicotinamide mononucleotide adenosine transferase NMNAT and a genetically engineered bacterium containing NAD kinase are used as a whole-cell catalyst, nicotinamide mononucleotide NMN is used as a starting substrate, adenosine triphosphate, polyphosphate and divalent metal ion salt are added, and the whole-cell enzyme is prepared through a one-pot method. The oxidized nicotinamide adenine dinucleotide phosphate NADP < + > is efficiently and catalytically synthesized by a one-pot method, and the whole-cell catalytic reaction liquid is separated and purified to obtain the NADP < + >. The double-enzyme catalysis method disclosed by the invention is short in reaction route, high in atom economy, capable of reducing the existing production cost, short in production period, easy for downstream separation and purification, and suitable for large-scale and large-scale production of NADP < + >.

The invention discloses a method for producing nicotinamide mononucleotide through double-enzyme'one-pot process' catalysis. According to the method, under the optimal conditions that EBY-NRK with the concentration of 25 mg / mL, EBY-PPK with the concentration of 10 mg / mL, NR with the concentration of 5 mM, ATP with the concentration of 1.5 mM, a phosphoric acid donor with the concentration of 15 mM and a Mg < 2 + >-containing substance with the concentration of 2 mM can be catalytically generated into 82.8% of NMN at the temperature of 30 DEG C and the pH value of 6.5, the ATP cost is reduced by 75%, and after a whole-cell enzyme preparation is repeatedly used for five times, the enzyme activity is still larger than 50%. The invention further develops a whole-cell catalyst displayed on the surface of the recombinant yeast, the problem of conversion inhibition caused by accumulation feedback of by-products in the NMN biosynthesis process is solved by coupling an ATP regeneration system, and the cost is effectively reduced while a good NMN product generation rate is obtained.

A nutraceutical composition, consisting of Nicotinamide Mononucleotide in a range 250-1000 mg, Spermidine in a range 0.5-10 mg, Rhodiola Rosea in a range 200-600 mg, Cacao in a range 1000-10,000 mg, L-Theanine in a range 50-200 mg, Glucosamine in a range 500-3000 mg, and Hyaluronic acid in a range 50-240 mg, or consisting of Epigallocatechin-3-gallate in a range 100-500 mg, Quercetin in a range 250-1000 mg, Resveratrol in a range 150-1000 mg, Sulforaphane in a range 1-50 mg, and Calcium Alphaketoglutarate in a range 300-2000 mg, or consisting of Microdose Lithium in a range 1-5 mg, Trimethylglycine in a range 500-2000 mg, Glutathione in a range 250-1000 mg, Fisetin in a range 100-1000 mg, Urolithin A in a range 250-2000 mg, Sodium Butyrate in a range 100-3000 mg, Berberine in a range 500-1500 mg, Apigenin in a range 50-500 mg, Cinnamon in a range 1000-6000 mg, and Malate in a range 1000-6000 mg.

The invention discloses a long-acting anti-aging composition capable of quickly removing dark circles and application of the composition. The composition is prepared from the following components: beta-nicotinamide mononucleotide, glutathione, acetyl hexapeptide-8, acetyl tetrapeptide-5, snake venom-like peptide, arginine / lysine polypeptide, tocopheryl acetate and ergothioneine. According to the present invention, multiple functions are adopted, such that the effects of dark circle removing, repair, elasticity recovery, aging resistance and the like are provided so as to block the generation of dark circle, and the normal function of skin is ensured. The composition disclosed by the invention has the effect of quickly removing dark circles, and when the eye cream contains the composition, the eye cream can effectively remove the dark circles and has an obvious and quick effect within 15 minutes; after being used for a long time, the eye mask can remove dark circles and play a good control role.

The invention provides a nicotinamide phosphoribosyltransferase mutant and an application of the nicotinamide phosphoribosyltransferase mutant in preparation of beta-nicotinamide mononucleotide. According to the nicotinamide phosphoribosyltransferase mutant, 259th lysine (K) of an amino acid sequence shown in SEQ ID NO: 2 is mutated into 259th valine (V). The invention overcomes the technical problems of slow reaction, low yield, high energy consumption and NMN feedback inhibition of the product in the process of producing NMN by escherichia coli, and the constructed nicotinamide phosphoribosyltransferase mutant expression system is used for fermentation production of nicotinamide mononucleotide, and slows down the feedback inhibition effect.

The invention discloses a double-ligand Eu-MOF and application thereof in detection of nicotinamide mononucleotide, and belongs to the technical field of analysis and detection. The preparation method of Eu-TATB-BDC-NH2 comprises the following steps: dissolving 2, 4, 6-tri (4-carboxyphenyl)-1, 3, 5-triazine, 2-aminoterephthalic acid and europium nitrate hexahydrate in a mixed solution of water and N, N-dimethylformamide (DMF), adding nitric acid to adjust the pH value, ultrasonically dispersing uniformly, reacting in a high-pressure reaction kettle (polytetrafluoroethylene lining), cooling, centrifuging, washing and drying to obtain pink solid Eu-TATB-BDC-NH2; when the Eu-MOF is used for detecting the NMN, the method is simple, convenient, rapid and high in sensitivity, and a new strategy is provided for detecting the NMN in an actual sample.

The invention relates to a compound NMNH calcium salt, in particular to an amorphous form of a reduced β-nicotinamide mononucleotide calcium salt, a preparation method therefor, a use thereof as a pharmaceutical ingredient, a health care product ingredient and a cosmetic ingredient or as a food additive, and a formulation containing the salt, belonging to the field of medicines, health care products, cosmetics and food additives. Specifically, described in the invention is a NMNH calcium salt amorphous compound. The compound shows long-term continuous stability, and compared with NMNH disodium salt, has significantly superior oxidation resistance and stability, and anti-hygroscopic properties, and is more beneficial to long-term storage, promotion and market application. The NMNH calcium salt amorphous compound is simple in preparation process, easy to control and suitable for large-scale production.

The invention relates to a compound NMNH calcium salt, in particular to a polymorphic substance of a reduced beta-nicotinamide mononucleotide calcium salt, a preparation method of the polymorphic substance, application of the polymorphic substance as a pharmaceutical ingredient, a health care product ingredient, a cosmetic ingredient or a food additive, and preparations containing the salts. Belongs to the fields of medicines, health-care products, cosmetics and food additives. Specifically, the invention describes an NMNH calcium salt polymorphic substance, the crystal shows long-term continuous stability, the stability performance and moisture absorption resistance of the crystal are obviously superior to those of NMNH disodium salt, and the crystal is more beneficial to long-term storage, popularization and marketing. The preparation process of the NMNH calcium salt crystal is simple, easy to control and suitable for large-scale production.

The invention is applied to agricultural production, and particularly relates to a biostimulant formula and application of the biostimulant formula in promoting or regulating crop growth and inducing disease resistance in agricultural production. The biostimulant active component contains lactulose and nicotinamide mononucleotide; wherein the weight ratio of lactulose to nicotinamide mononucleotide is (1-10): (1-10). The formula of the biostimulant has the functions of promoting crop growth, improving crop quality and inducing disease resistance at the same time. The raw materials required by the formula are low in price and easy to obtain, the preparation process is simple, the equipment requirement is low, and the formula can be applied to the field of agricultural production.

The invention relates to a natural plant functional component based on cell function regulation, a natural plant composition and application thereof. The natural plant composition is prepared from the following raw material components in parts by weight: 1000 to 1600 parts of phospholipid, 200 to 400 parts of cholesterol, 200 to 400 parts of prinsepia utilis royle oil, 100 to 300 parts of ergothioneine and 200 to 500 parts of nicotinamide mononucleotide. Compared with the prior art, the composition not only can cover multi-target composite components with ROS removal, mitochondrial autophagy activation and NAD + level improvement, but also can improve the component permeability by optimizing the delivery technology, thereby effectively improving the skin anti-aging effect and the like.

The present invention describes a composition comprising an effective amount of a combination of two or more components selected from acacetin, ACT1 peptide, α-lipoic acid, alprostadil, anisomycin, apigenin, ascorbic acid, astragalus, berberine, β-lapachone, β-hydroxy-β-methylbutyrate, bacopa monnieri, catechins, catechols, chamomile, chrysin, coumestrol, curcumin, dinitrophenol, dinoprost, ellagic acid, (-)-epigallocatechin gallate, green tea extract, fisetin, genistein, ginsenoside RE, glabridin, 18-α-glycyrrhetinic acid, 18-β-glycyrrhetinic acid, glycyrrhizin, hydroquinone, isoquercetin (EMIQ), shan Phosphate, black bean anthocyanins, leucine, lithium, luteolin, luteolin, luteolin, melatonin, menadione, 1‑methylnicotinamide (MNA), methyl salicylate, myricetin, nicotinamide adenine dinucleotide, nicotinic acid (vitamin B3), nicotinamide (NAM), nicotinamide mononucleotide (NMN), nicotinamide riboside (NR), nicotinic acid adenine dinucleotide (NaAD), nicotinic acid mononucleotide (NaMN), parsley, phenylephrine, pokeweed mitogen, 15‑Δ prostaglandin J2, puromycin, quercetin, quinolinic acid, retinoic acid, trichostatin A, troxerutin, rutin, tryptophan, vitamin D3, withaferin A, wortmannin, and zinc (including its salts).

The invention relates to a compound NMNH calcium salt, in particular to an amorphous form of a reduced β-nicotinamide mononucleotide calcium salt, a preparation method therefor, a use thereof as a pharmaceutical ingredient, a health care product ingredient and a cosmetic ingredient or as a food additive, and a formulation containing the salt, belonging to the field of medicines, health care products, cosmetics and food additives. Specifically, described in the invention is a NMNH calcium salt amorphous compound. The compound shows long-term continuous stability, and compared with NMNH disodium salt, has significantly superior oxidation resistance and stability, and anti-hygroscopic properties, and is more beneficial to long-term 10 storage, promotion and market application. The NMNH calcium salt amorphous compound is simple in preparation process, easy to control and suitable for large-scale production.

The invention provides a red ginseng extract special care essence and a preparation method thereof, and a red ginseng extract composition. The red ginseng extract composition comprises a red ginseng extract, deer bone collagen peptide, a red camellia extract, beta-nicotinamide mononucleotide, a rose extract, a moutan bark extract and a ligusticum wallichii extract. According to the invention, the red ginseng extract, the deer bone collagen peptide, the red camellia flower extract, the beta-nicotinamide mononucleotide, the rose flower extract, the moutan bark extract and the ligusticum wallichii extract are adopted, so that the prepared special care essence with the red ginseng extract has better oxidation resistance and aging resistance.

The invention belongs to the field of biopharmacy and biotransformation, and particularly relates to a nicotinamide mononucleotide adenosine transferase mutant and application thereof. According to the invention, NMN is taken as a substrate, ATP disodium salt is taken as an auxiliary substrate, and NAD < + > is prepared under the catalysis of the nicotinamide mononucleotide adenosine transferase mutant under the action of magnesium ions, so that the enzymatic activity is improved, few byproducts are generated, and the reaction rate is high. The nicotinamide mononucleotide adenosine transferase mutant disclosed by the invention has better catalytic efficiency, and when NMN is taken as a substrate for catalytic reaction, the conversion rate is higher than that of an original enzyme, and the NAD < + > yield is also increased to a certain extent. The process overcomes the defects of various process steps, harsh reaction conditions, difficulty in separation and purification and the like of a chemical method for synthesizing NAD < + >, and is an efficient, low-cost and environment-friendly production mode.

The invention discloses a targeted energy metabolism composition for improving the anti-aging effect of skin as well as a preparation method and application of the targeted energy metabolism composition. The targeted energy metabolism composition is prepared from the following components in parts by mass: 0.1 to 5 parts of saccharomycetes rice fermentation filtrate, 0.1 to 5 parts of a tremella aurantialba sporocarp extract and 0.01 to 5 parts of nicotinamide mononucleotide NMN liposome. According to the targeted energy metabolism composition for improving the anti-aging effect of the skin and the preparation method and application of the targeted energy metabolism composition, effective permeation and precise delivery of functional components can be achieved, and the anti-aging, whitening and moisturizing effects of the skin are improved.

The invention relates to crystalline forms of a β-nicotinamide mononucleotide, methods of their preparation, and related pharmaceutical preparations thereof. The invention also relates to preparations suitable for nutraceutical, veterinary, and agriculturally-relevant uses.

The invention relates to an application of beta-nicotinamide mononucleotide (NMN) in prevention and treatment of liver injury caused by aflatoxin B1 (AFB1), the NMN has a good protection effect on the liver injury caused by exposure of the AFB1, and particularly, the serum transaminase activity and liver tissue pathological injury of a mouse using an NMN + AFB1 group are improved compared with those of a mouse in the AFB1 group, and the NMN has a good protection effect on the liver injury caused by exposure of the AFB1 group. Namely, the NMN has a certain protection effect on the mouse liver injury induced by exposure of the AFB1; the content of IL-6, IL-1beta and TNF-alpha in the liver of a mouse in an AFB1 group is remarkably increased, and the expression of IL-6, IL-1beta and TNF-alpha in the liver of the mouse infected with AFB1 is remarkably reduced by the NMN, namely, the NMN relieves the inflammatory response induced by the AFB1. The NMN can effectively relieve the development of mouse AFB1 subchronic poisoning and gradually recover the mouse AFB1 subchronic poisoning to a normal level, so that a new clue and an experimental basis are provided for research and development of an additive or a medicine for resisting AFB1 poisoning.

The invention relates to the technical field of pharmaceutical preparations, and discloses a biological beauty skin care product containing nicotinamide adenine dinucleotide (NAD < + >) and cell exosomes, and the biological beauty skin care product comprises the following components by weight: 10-50 parts of a pH responsive nicotinamide mononucleotide liposome; 1 to 10 parts of cell exosome sustained release microspheres; 0.1 to 2 parts of ergothioneine; 0.1 to 1 part of copper peptide; 1 to 10 parts of phytosphingosine; 0.01 to 0.5 part of water-soluble fullerene; 1-8 parts of 1, 2-pentanediol as a transdermal enhancer; 0.05 to 0.5 part of acetylated polypeptide-8; wherein the particle size of the pH response type nano-liposome is 50-200 nm, and the particle size of the hyaluronic acid microspheres is 100-500 nm, so that the skin health is promoted, and the metabolic function is regulated.

The invention discloses a method for detecting the content of nicotinamide mononucleotide, ergothioneine and nicotinamide. According to the method, the content of nicotinamide mononucleotide, ergothioneine and nicotinamide in a sample is analyzed by adopting a high performance liquid chromatography; according to the method, a CAPCELL PAK ADME chromatographic column is used, and the molecular weight of the CAPCELL PAK ADME chromatographic column is 100 And an acetonitrile-buffer solution is used as a mobile phase. Chromatographic peaks obtained by the method are sharp and symmetrical in shape, good in area reproducibility, good in separation degree, simple, rapid, high in specificity, short in detection period and high in sensitivity; the contents of nicotinamide mononucleotide, ergothioneine and nicotinamide in a sample can be rapidly, efficiently, accurately and simultaneously detected.

The utility model discloses a raw material oscillation device for beta-nicotinamide mononucleotide production, and belongs to the technical field of beta-nicotinamide mononucleotide production. The raw material oscillation device for beta-nicotinamide mononucleotide production comprises an oscillation base and an oscillation platform, a fixing plate is arranged in the oscillation base, a driving motor is installed below the fixing plate, the output end of the driving motor is connected with a hexagonal shaft, the hexagonal shaft is sleeved with a rotating disc, and a first wave face is arranged on the lower surface of the rotating disc. According to the raw material oscillation device for beta-nicotinamide mononucleotide production, the driving motor controls the rotating disc to rotate, under the action of the first wave surface, the second wave surface, the first limiting ball and the second limiting ball, vertical vibration and multidirectional inclined superposition are achieved, jolting and swinging composite motion is formed, the actually measured mixing uniformity is improved, and the production efficiency is improved. The wave crest number of the first wave surface is integral multiples of the number of the first limiting balls, so that the vertical vibration synchronism is ensured.