149 results about "Chaotropic agent" patented technology

The present invention is generally directed to methods of producing an increase in the enrichment or recovery of preferred forms of IgG proteins. More particularly, the invention relates to subjecting preparations of such recombinant IgG proteins with a reduction / oxidation coupling reagent and optionally a chaotropic agent.

This invention provides a method of inactivating human noroviruses and other acid stable viruses. The method includes the step of contacting the virus with a virucidally-enhanced alcoholic composition that includes an alcohol, and an enhancer selected from cationic oligomers and polymers, chaotropic agents, and mixtures thereof.

This invention provides a method of inactivating non-enveloped virus particles. The method includes the step of contacting the virus with a virucidally-enhanced alcoholic composition that includes an alcohol, and an enhancer selected from the group consisting of cationic oligomers and polymers, proton donors, chaotropic agents, and mixtures thereof.

This invention provides a method of inactivating non-enveloped virus particles. The method includes the step of contacting the virus with a virucidally-enhanced alcoholic composition that includes an alcohol, and an enhancer selected from the group consisting of cationic oligomers and polymers, proton donors, chaotropic agents, and mixtures thereof.

The present invention is generally directed to methods of producing an increase in the enrichment or recovery of preferred forms of IgG proteins. More particularly, the invention relates to subjecting preparations of such recombinant IgG proteins with a reduction / oxidation coupling reagent and optionally a chaotropic agent.

The present invention is directed to a method for adsorbing, i.e. non-covalently binding, nucleic acids to a solid phase using a two-step procedure. Furthermore, the present invention pertains to a method for isolating nucleic acids from a biological sample. In the first step of the procedure, lysis is effected by mixing the biological sample with an aequous lysis buffer containing a chaotropic agent and incubating the mixture; in the second step, the concentration of the chaotropic agent in the mixture is increased and the mixture is contacted with the solid phase, whereby the nucleic acids in the liquid phase is adsorbed to the solid phase.

The present invention relates to a technique for efficient isolation of long nucleic acids and short nucleic acids from a sample containing long nucleic acids and short nucleic acids via safe and convenient operations. Specifically, long nucleic acids and short nucleic acids are isolated from a sample containing nucleic acids by mixing a chaotropic agent with the sample containing nucleic acids, allowing the mixed solution to pass at least twice through a first solid phase containing silica that has passage pores having predetermined pore sizes, allowing the mixed solution to pass at least twice through a second solid phase containing silica that has passage pores having pore sizes smaller than those of the first solid phase containing silica, and separately recovering nucleic acids that have bound to the first solid phase containing silica and those that have bound to the second solid phases containing silica.

The present invention is directed to a method for adsorbing, i.e. non-covalently binding, nucleic acids to a solid phase using a two-step procedure. Furthermore, the present invention pertains to a method for isolating nucleic acids from a biological sample. In the first step of the procedure, lysis is effected by mixing the biological sample with an aqueous lysis buffer containing a chaotropic agent and incubating the mixture; in the second step, the concentration of the chaotropic agent in the mixture is increased and the mixture is contacted with the solid phase, whereby the nucleic acids in the liquid phase is adsorbed to the solid phase.

A method for purifying a protein using Protein A chromatography comprising a) absorbing the protein to Protein A immobilized on a solid support; b) removing contaminants by washing the immobilized Protein A containing the absorbed protein with a buffer comprising one or more chaotropic agents in combination with one or more hydrophobic modifiers and having a pH of at least 7.0; and c) eluting the protein from the Protein A immobilized on the solid support.

Treated peroxycarboxylic acid compositions and methods of using the same are provided to eliminate or reduce malodors associated therewith. Peroxycarboxylic acid compositions are treated with odor reducing agents, including various chaototropic agents. The invention further relates to methods employing the reduced odor peroxycarboxylic acid compositions.

The invention relates to a troponin I latex-enhanced immunological turbidimetry detection kit which particularly comprises a first reagent and a second reagent. The first reagent comprises buffer solution, blocking agents, surface active agents, electrolyte, stabilizers, polyethylene glycol, chaotropic agents and preservatives; the second reagent comprises latex particles coated with human troponin I monoclonal antibodies, buffer solution, surface active agents, stabilizers and preservatives. The kit displays better interference resistance.

The invention provides methods for removing a contaminant or inhibitor from a nucleic acid-comprising sample, wherein the contaminant or inhibitor inhibits the amplification or hybridization of the nucleic acid in the sample, or inhibits an enzymatic reaction utilizing the nucleic acid in the sample, the method comprising the steps of: (a) providing a reaction mixture comprising the sample, a chaotropic agent, ammonium acetate or an equivalent, and a detergent, (b) isolating the nucleic acid and remaining contaminants and inhibitors from the reaction mixture in a supernatant; and (c) contacting the nucleic acid supernantant with a flocculant resulting in the further removal of the contaminant or the inhibitor from the supernatant. The invention also provides kits that comprise the components necessary to carry out the method.

The invention relates to a high-efficiency extraction agent for extracting H acid waste liquid, which is prepared from the following raw materials in parts by weight: 2-5 parts of complexing agent, 4-10 parts of diluting agent and 0.5-1.5 parts of chaotropic agent, wherein the mixing temperature of the complexing agent, the diluting agent and the chaotropic agent is 25-60 DEG C. The extraction ratio and the back extraction ratio reach more than 99%, and the extraction agent can be recycled. After extraction, the sewage is clear and transparent and can be used as bottom water, dilution water and washing water in production, thereby achieving the purpose of zero discharge of the sewage. The extraction agent has high efficiency and universality in the aspect of extracting mother liquor of H acid products.

The invention discloses a process for treating dinaphthol wastewater by using a complexation extraction method. A process core includes that trioctylamine or trialkyl tertiary amine serves as a complexing agent, sulfonated kerosene serves as a diluent, and chloric acid tributyl ester serves as a chaotropic agent to be mixed according to a certain ratio into an extraction agent, the extraction agent comprises, by volume, 20% to 30% of the complexing agent, 60% to 70% of the diluent and 5% to 15% of the chaotropic agent. Dinaphthol production wastewater is extracted when the temperature is in a range of 20 DEG C to 30 DEG C and the pH is 1, aqueous phase: oil phase is 1: 1 (volume ratio), the removal rate of first extraction CODcr can reach 97%, naphthalene sulfonate in wastewater can be extracted through multistage extraction, and simultaneously water is discharged up to standard. Water phases of back extraction can be recycled as production materials; and organic phases are mixtures of the extraction agent and the diluent and can be recycled.

The present invention provides a universally applicable lysis buffer comprising a chaotropic 5 agent, a reducing agent, and a proteolytic enzyme suitable for processing a wide variety o different sample types, such as different types of bodily samples relevant for the diagnosis o a respiratory disease. Furthermore, the present invention provides the use of a chaotropic agent, a reducing agent, and a proteolytic enzyme for the lysis of a broad spectrum of bodily samples. Moreover, the present invention provides a method for processing bodily samples which is universally applicable to the lysis of a variety of different types of bodily samples. Furthermore, the present invention provides methods for analyzing a bodily sample or for detecting the presence of a pathogen in a bodily sample, preferably, for diagnosing a respiratory disease, such as pneumonia or tuberculosis. Preferably, these methods are universally applicable to a variety of sample types, are applicable as one-tube-processes, are 15 suitable for performance in a high-throughput setting, and are automatable.

The present invention relates to methods for preparing an isolated biological sample containing at least one of DNA and RNA, such that the DNA and / or RNA is preserved in the sample at ambient temperatures for at least thirty days, the method comprising: contacting the isolated biological sample with a composition comprising a chaotropic agent, and subjecting the contacted sample to microbial cell lysis; and optionally, contacting the lysed biological sample with a slurry of size-selected silicon dioxide to form at least one of DNA-silicon dioxide complexes or RNA-silicon dioxide complexes in the sample; isolating at least one of DNA-silicon dioxide complexes or RNA-silicon dioxide complexes from the sample; and, separating at least one of DNA and RNA from the silicon dioxide and collecting at least one of the DNA and RNA.The present invention further relates to methods for preparing an isolated biological sample, the method comprising, separating the components in an isolated biological sample according to their size, wherein the components are at least one of DNA and RNA; purifying and isolating SSU rRNA from the biological sample using a composition comprising a ribonuclease inhibitor and a deoxyribonuclease to remove DNA from the sample, reverse transcribing the SSU rRNA into ds cDNA using random primers for SSU rRNA.The present invention also relates to computer implemented methods comprising, receiving an isolated sample prepared according to the methods of the invention, sequencing the sample, and providing the sequence with a sequence identifier (ID), the sequence comprising a plurality of groups of k-mers, each group of k-mers defining a node in a multi-level hierarchy which defines the relationship between the groups of k-mers; providing each group of k-mers with a respective group identifier (ID), determining the frequency of the k-mers in each group; generating a group signature array for each group of k-mers, each group signature array comprising the k-mers in each group that have the most increased frequency compared with the sibling k-mers; generating a signature map comprising each group signature array and at least one of the identifiers, the identifier of at least one parent group and the identifier of at least one child group; and outputting the signature map to be used to classify the sequence.

A novel approach is described for reversing aggregation and increasing refolding by application of hydrostatic pressure. A protein of interest in an aggregated, or inclusion body, or other non-native or inactive state is subjected to high hydrostatic pressure. This treatment denatures the protein to states (or conformations) competant for refolding and results in increased formation of native protein once pressure is released. The technique can facilitate conversion non-native proteins, including inclusion bodies and aggregates to native proteins without addition of chaotropic agents, changes in buffer, or large-scale dilution of reagents required for traditional refolding methods.

A method of sanitizing chromatographic media is provided. The method includes contacting the media with an acidic chaotropic agent, at low temperature and low pH. The method provides pathogen removal and / or inactivation, including viral inactivation in particular embodiments.

This invention provides a method of inactivating non-enveloped virus particles. The method includes the step of contacting the virus with a virucidally-enhanced alcoholic composition that includes an alcohol, and an enhancer selected from the group consisting of cationic oligomers and polymers, proton donors, chaotropic agents, and mixtures thereof.

The invention provides a virus sample preservation solution as well as a preparation method and application thereof, and relates to the technical field of biology. The virus sample preservation solution provided by the invention is prepared from a buffer compound with specific concentration, a chaotropic agent, a detergent and a chelating agent. Through the mutual matching of the specific components with the specific concentration, the virus sample preservation solution provided by the invention has the advantages of safety, stability and high efficiency; viruses can be inactivated within 10 minutes; the virus sample nucleic acid can be stably preserved. In addition, the preservation on the virus sample can be realized without ultra-low temperature environment; and the virus sample preservation solution can be applicable to the virus sample collection and transportation in clinic and field environment.

The present invention relates to the solubilization and recovery in high yield, of inclusion body proteins from host cells using an appropriate denaturating agent. The process avoids the use of high concentration of chaotropic agents such as guanidine hydrochloride or urea.

The invention relates to a fluorescence dye for detecting fungus and dermatozoon. The technical problems that three dyeing liquid ingredients of a current fluorescence dye method cannot stably coexist for a long time, and need to be prepared and stored respectively, operating is tedious, the dyeing time is long, limitation exists in detected samples, some samples with the small impurities can only be dissolved, and the clinical application range is limited are solved. The fluorescence dye is prepared from water, a fluorescent whitening agent, alkali, a background redyeing agent, a chaotropic agent and a moisturizing agent. As the fluorescence dye is a single dosage form, the fluorescent whitening agent, strong alkali and the background redyeing agent can stably coexist; the stability of a product is good, dyeing can be completed only through one step, clinical using is more convenient and fast, original detection which can be completed in 5 minutes or above can be completed in tens of seconds through the fluorescence dye, and the detection efficiency of the fungus and the dermatozoon is greatly improved.

The invention discloses a preparation method of ternary copolymerized cationic polyacrylamide, including the following steps: stirring to dissolve cationic monomer diallyl dimethyl ammonium chloride,methacrylamide, propyl trimethylammonium chloride, nonionic monomer acrylamide, a metal ion chelating agent, and a chaotropic agent in water, adding a chain transfer agent and an initiating agent in turn after injecting nitrogen for removing oxygen, which evenly mixes the chain transfer agent and initiating agent and reaction liquid by effect of the nitrogen flow to obtain the ternary copolymerized cationic polyacrylamide colloid product, obtaining the colloidal particles by prilling the colloid product, adding anti-crosslinking agent solution into the colloidal particles for mixing, drying and milling the colloidal particles into powder, after sieving, obtaining powder product of the ternary copolymerized cationic polyacrylamide. The ternary copolymerized cationic polyacrylamide has significant hydrolysis resistance property, flocculent property is maintained and improved, the product economy is optimized relative to copolymerized products of MAPTAC and acrylamide. The method is applicable to large kettle production, which is easy to implement industrialization.

A novel approach is described for reversing aggregation and increasing refolding by application of hydrostatic pressure. A protein of interest in an aggregated, or inclusion body, or other non-native or inactive state is subjected to high hydrostatic pressure. This treatment denatures the protein to states (or conformations) competant for refolding and results in increased formation of native protein once pressure is released. The technique can facilitate conversion non-native proteins, including inclusion bodies and aggregates to native proteins without addition of chaotropic agents, changes in buffer, or large-scale dilution of reagents required for traditional refolding methods.

A homogeneous agglutination immunoassay method wherein a sample possibly containing the analyte to be measured is mixed with a binding partner for the analyte and with at least one chaotropic agent, monovalent ions in form of at least one salt, or with a combination of at least one chaotropic agent and monovalent ions in form of at least one salt, measuring at least one signal related to interactions of the binding partner with the analyte over a reaction time and calculating from at least one of the signals measured the concentration of analyte in the sample.

The present invention belongs to the technical field of functional food preparation and discloses a protein self-assembly embedding insoluble active material nano product and a preparation method thereof. Proteins are dispersed in water to obtain a protein dispersion solution; then a chaotropic agent is added for a reaction to obtain a denatured protein solution; insoluble active materials are directly added or added into the denatured protein solution before being dissolved in a chaotropic agent aqueous solution; the materials are stirred and mixed evenly before reaction; after the reaction,the chaotropic agent is removed using dialysis; and insoluble materials are removed by centrifugation to obtain the protein self-assembly embedding insoluble active material nano product. The structural characteristic of the protein and the chaotropic agent of urea are utilized to induce encapsulation and embedding of hydrophobic materials by the protein; based on the premise of not adding any organic solvents, a high-stability insoluble active material aqueous solution can be successfully obtained to form the high-load protein nano product.

A method for purifying a protein using Protein A chromatography comprising a) absorbing the protein to Protein A immobilized on a solid support; b) removing contaminants by washing the immobilized Protein A containing the absorbed protein with a buffer comprising one or more chaotropic agents in combination with one or more hydrophobic modifiers and having a pH of at least 7.0; and c) eluting the protein from the Protein A immobilized on the solid support.

To encapsulate indocyanine green (ICG) at a high concentration in a liposome without causing the agglomeration of ICG so that ICG remains as a monomer in an ICG-containing particle to be used as, for example, a contrast agent for fluorescence imaging or photoacoustic imaging, provided is a method of producing an indocyanine green-containing particle, including the step of mixing indocyanine green, a particle, and a solution containing 1 mM or more and 10 M or less of a chaotropic agent.

This invention discloses a fuel which substitutes diesel oil, the fuel contains methanol, diesel oil, additive for combustion, potentiator, the weight and shares ratio of used methanol, diesel oil, additive for combustion and potentiator are better selected as 50-95:5-10:0.2-1: 0.2-0.7. The fuel which substitutes diesel oil of this invention is optimized from what contain one or more kinds of what is selected from accessory ingredient such as: chaotropic agent, carbon reduction agent, limiting smoke agent, removing flavor agent, cleaner, noise reduction agent and antifreezer as so on..