24 results about "Chaotropic agent" patented technology

The present disclosure provides methods for isolating nucleic acids from a sample, comprising: (a) contacting a sample, a lysate of the sample, a supernatant of the lysate, or a portion of the sample, the lysate or the supernatant with one or more first agents (e.g., protein precipitating agents) and one or more second agents (e.g., inhibitor removing agents) to generate a mixture, (b) separating the mixture of step (a) into a solid phase and a liquid phase, wherein the one or more second agents are primarily in the solid phase, and (c) isolating nucleic acids from the liquid phase of step (b). Compositions and kits useful in such methods are also disclosed. Further disclosed are methods, compositions and kits for preparing a lysate using a lytic reagent comprising one or more relatively mild chaotropic agents and one or more phosphates from a sample, especially a complex sample, such as a soil or stool sample.

The invention relates to a unified system for the extraction and purification of biomolecules using a novel Cloudy-Precipitated (CP) Buffer and an integrated extraction device. The CP Solution remains cloudy even when heated and enables single- or multi-step workflows for isolating nucleic acids without requiring multiple buffers or organic solvents. It contains optimized concentrations of detergents, chaotropic agents, salts, and adsorbents to efficiently lyse cells and minimize processing steps. The associated device incorporates a heating and filtration system that allows separation of target molecules from inhibitors or impurities based on molecular weight. It can operate as a single- or double-step configuration with various filter membrane options. This system reduces time, labor, and environmental impact while enhancing sample quality for molecular diagnostics, research, and therapeutic applications.

Protein hydrolysate compositions and methods of making the same are disclosed. The protein hydrolysate composition has a protein-rich organic content. The protein hydrolysate composition may be substantially free of exogenous chelating agents, chaotropic agents and surfactants. The protein hydrolysate composition may be low in ash content. The protein hydrolysate composition is produced by processing a biomass, e.g., a microbial biomass, through a combination of physical, chemical and / or enzymatic treatments. The protein hydrolysate may be sourced via microbial biomass from CCk as a carbon source. Also disclosed are methods of using the protein hydrolysate compositions, e.g., as a biostimulant.

The invention relates to the technical field of nucleic acid detection, in particular to a lysis buffer solution, a kit and an extraction method for extracting free DNA of peripheral blood. The lysis buffer solution for extracting the free DNA of the peripheral blood comprises a first lysis solution and a second lysis solution, the first lysis solution takes guanidine hydrochloride as a main chaotropic agent and at least one of ammonium thiocyanate and guanidine isothiocyanate as an auxiliary chaotropic agent, and is compounded with a metal ion chelating agent, trehalose, dithiothreitol, digestive enzyme, sodium dodecyl sarcosinate, borate and ascorbic acid; the second lysis solution takes guanidine hydrochloride as a main chaotropic agent and urea as an auxiliary chaotropic agent, and is compounded with a metal ion chelating agent, trehalose, dithiothreitol, digestive enzyme, sodium dodecyl sarcosinate, borate, ascorbic acid and polyethylene glycol. The buffer solution of the lysate does not contain SDS (sodium dodecyl sulfate), so that the residue of SDS in an extracted product can be avoided, and the amount of extracted free DNA (deoxyribonucleic acid) is more.

The present disclosure provides compositions and methods for sample processing, particularly for oligonucleotide analysis e.g. analysis of formulated nucleic acid drugs. A composition for pretreating at least one target nucleic acid in a biological mixture provided herein includes a chaotropic agent selected from a substituted guanidine, a substituted amidine, a substituted quaternary amine, or a combination thereof, an optional protease, and / or an optional disulfide-reducing agent. Methods of analyzing at least one target nucleic acid in a biological mixture is also provided herein. Furthermore, the present disclosure provides methods for quantifying at least one target cationic lipid interacting with a nucleic acid.

The invention provides a modified dolomite powder, a preparation method thereof, and a concrete. The modified dolomite powder is prepared from 98% to 99% of a dolomite powder, 0.2% to 0.5% of a chaotropic agent, 0.6% to 1.0% of a dihydrogen phosphate, and 0.2% to 0.5% of a capillary filler according to the mass percentage. The chaotropic agent is one or more of sodium sulfate, potassium sulfate, and ammonium sulfate. By promoting the dissolution of the surface of the dolomite powder, participating in the hydration reaction, and filling capillary pores, the triple modification solves the problems of bleeding, strength, and durability of the dolomite powder concrete in the related art. The obtained modified dolomite powder has good solubility and high chemical activity, and the prepared concrete has high strength and compactness, low porosity, and good durability.

The present disclosure relates to a method for producing an extracellular matrix hydrogel, the method comprising (a) decellularizing a tissue sample to form a decellularized tissue mesh; (b) depolymerizing the decellularized tissue mesh to form a depolymerized protein dispersion, the depolymerizing comprising mixing the decellularized tissue mesh with a depolymerizing solution comprising a chaotropic agent to form a depolymerizing mixture; mechanically homogenizing the depolymerizing mixture at a chilled temperature to form a homogenized mixture; separating the homogenized mixture into (a) a waste precipitate and (b) a supernatant comprising the depolymerized protein dispersion; (c) filtering the depolymerized protein dispersion to form a sol; and (d) gelling the sol to form the extracellular matrix hydrogel, the gelling comprising combining the sol with a gelling solution comprising a kosmotropic agent; and separating a fluid phase away from the extracellular matrix hydrogel as it forms.

The present invention provides an improved manufacturing process for producing a bispecific antibody product comprising at least two binding domains. The process comprises at least a process comprising a step of refolding a construct, wherein one or more domains comprise disulfide bonds, and wherein the construct is contacted with a refolding buffer to refold the construct into its native form, the buffer comprising (i) an oxidizing and / or reducing agent at a concentration of at least 0.1 mM, and (ii) a chaotropic agent at a concentration > 1 M, and wherein the pH of the refolding buffer corresponds to + / - 4.5 of the pi value of the first domain or + / - 4.0 of the pi value of the entire construct.

The invention relates to the technical field of cosmetics, and discloses a silk-core protein fiber aggregate, a silk-core protein carrier and a preparation method and application thereof.The preparation method of the silk-core protein fiber aggregate comprises the steps of mixing, specifically, an extract obtained after degumming of silk and a chaotropic agent aqueous solution are mixed according to the volume ratio, and a silk extract aqueous solution is obtained; a dissolving step: placing the silk extract aqueous solution in a constant-temperature water bath, and continuously magnetically stirring until the silk fibroin is completely dissolved to obtain a dissolved silk fibroin solution; and a cultivation step: carrying out concentration-dilution-temperature cultivation treatment on the silk fibroin solution to obtain the silk fibroin fiber aggregate containing the beta-folders. According to the technical scheme provided by the invention, the problem that an existing liposome carrier has defects in the aspects of stability and irritation can be solved.

Provided is a method for isolating nucleic acids from a plant sample comprising(a) preparing a lysed sample wherein preparing comprises(i) lysing a plant sample by mechanically disrupting the plant sample in a lysis solution which comprises at least one chaotropic agent and one or more solid disrupting particles, and(ii) optionally clearing the lysate;(b) contacting the lysed sample with at least one protein precipitating agent and at least one inhibitor removing agent and providing a mixture;(c) obtaining a liquid phase from the mixture; and(d) isolating nucleic acids from the liquid phase.Also provided is a kit for use in such method.

The invention relates to the technical field of biological peptides, and discloses a weight-losing peptide with high activity as well as a preparation method and application thereof, and the weight-losing peptide is prepared from an Arg34-GLP-1 (7-37) main chain, an acylating agent, an N-acetyl-L-arginine chaotropic regulator, a sodium benzoate interface regulator, a zinc salt masking agent and a reaction solvent. The preparation method comprises the following steps: carrying out in-situ coordination masking on the N-terminal of polypeptide by using zinc ions in a water phase, then feeding a reaction solvent to implement solvent polarity switching so as to construct a homogeneous reaction system, then carrying out high-selectivity acylation reaction, and finally carrying out metal decomplexation and deprotection treatment. According to the invention, the technical problems of serious N-terminal competitive side reaction, easy racemization of histidine and easy aggregation and precipitation of polypeptide in a mixed solvent in the modification process are effectively solved by utilizing the synergistic effect of metal coordination locking and a chaotropic regulator, and the obtained product is high in regioselectivity and complete in biological activity.

Disclosed herein are novel buffers for rapid and simplified nucleic acid extraction, and methods of their use. The buffers comprise a chaotropic agent, such as guanidine hydrochloride, sodium chloride, or lithium chloride, combined with Tris-EDTA buffer at a specific pH. The buffers facilitate efficient lysis of cells and viruses, inactivate nucleases, and enable binding of nucleic acids to various silica surfaces without the need for multiple wash steps or specialized equipment.

The invention belongs to the technical field of medicines, and particularly relates to application of combination of gelatin and chaotropic agent in preparation of a medicine for treating androgenetic alopecia. The invention provides a chaotropic agent-gelatin collaborative engineering strategy, and a protein microneedle composed of gelatin and a chaotropic agent is constructed. Through the screening preparation method, the protein microneedle has high mechanical properties and high skin puncture efficiency. When the protein microneedle is used for treating androgenetic alopecia, compared with a single NaI or gelatin protein microneedle, the protein microneedle has a synergistic effect in the aspects of improving new hair, improving the number of hair follicles and promoting hair follicles in a resting period to be converted into a growing period, and the effect of the protein microneedle is superior to that of minoxidil medicine; the combination of the gelatin and chaotropic agent has a good application prospect in preparation of the medicine for treating androgenetic alopecia.

The invention relates to a unified system for the extraction and purification of biomolecules using a novel Cloudy-Precipitated (CP) Buffer and an integrated extraction device. The CP Solution remains cloudy even when heated and enables single- or multi-step workflows for isolating nucleic acids without requiring multiple buffers or organic solvents. It contains optimized concentrations of detergents, chaotropic agents, salts, and adsorbents to efficiently lyse cells and minimize processing steps. The associated device incorporates a heating and filtration system that allows separation of target molecules from inhibitors or impurities based on molecular weight. It can operate as a single- or double-step configuration with various filter membrane options. This system reduces time, labor, and environmental impact while enhancing sample quality for molecular diagnostics, research, and therapeutic applications.

The application belongs to the technical field of medicine, and particularly relates to a chaotropic agent triggered fast absorbable protein microneedle, an anesthetic protein microneedle and a preparation method and application thereof. Iodine ions are selected as typical chaotropic agents, and gelatin is selected as a protein, and a new protein microneedle of interaction between the chaotropic agent and the protein is constructed through a two-step method. The protein microneedle has the characteristics of rheology, high mechanical strength and in-vivo fast absorption, and realizes the balance of 'processability, high mechanical strength and fast dissolution behavior'. The chaotropic agent triggered fast absorbable protein microneedle is applied to the fields of immunoregulation and local anesthesia respectively, and the system realizes the transition of the microneedle from a 'passive carrier' to an 'active therapeutic agent', and has a good application prospect.

The present disclosure relates to a method for preparing an extracellular matrix hydrogel, the method comprising (a) decellularizing a tissue sample to form a decellularized tissue web; (b) depolymerizing the decellularized tissue web to form a depolymerized protein dispersion, the depolymerizing comprising mixing the decellularized tissue web with a depolymerizing solution comprising a chaotropic agent to form a depolymerized mixture; mechanically homogenizing the depolymerized mixture at a cooling temperature to form a homogenized mixture; separating the homogenized mixture into (a) a waste precipitate and (b) a supernatant comprising the depolymerized protein dispersion; (c) filtering the depolymerized protein dispersion to form a sol; and (d) gelling the sol to form the extracellular matrix hydrogel, the gelling comprising combining the sol with a gelling solution comprising a lyophilic agent; and separating a liquid phase from the extracellular matrix hydrogel during the formation of the extracellular matrix hydrogel.

The present invention provides a method of isolating nucleic acids from a plant sample comprising: (a) preparing a lysed sample wherein the preparing comprises: (i) lysing the plant sample by mechanically disrupting the plant sample in a lysing solution comprising at least one chaotropic agent and one or more solid disruption particles, and (ii) optionally clarifying the lysate; (b) contacting the lysed sample with at least one protein precipitating agent and at least one inhibitor removing agent and providing a mixture; (c) obtaining a liquid phase from the mixture; and (d) isolating the nucleic acid from the liquid phase. A kit for use in the method is also provided.

The application discloses a pathogen enrichment treatment method for blood culture positive samples, and belongs to the field of blood sample treatment. The method provided by the application comprises the following steps: providing an enrichment diluent containing an aqueous solution of an alkaline chaotropic agent, wherein the aqueous solution of the alkaline chaotropic agent comprises an aqueous solution of thiourea and / or an aqueous solution of guanidine salt; mixing a positive blood culture sample with the enrichment diluent, discarding the supernatant and reserving the precipitate after standing and centrifugation; and resuspending the precipitate to obtain a pathogen concentrated product. The aqueous solution of the alkaline chaotropic agent selectively lyses blood cells and protects the morphology of pathogens, effectively removes background interference and efficiently enriches pathogens. The obtained concentrated product can be used in multiple scenes such as microscopic examination, molecular detection and drug sensitivity test, and can significantly improve the recognition degree of pathogens and the timeliness of reports in the microscopic examination scene. The method is simple to operate, has low biological safety risk, and has a wide clinical application prospect.

The present invention provides a method of recovering RNA from a sample, the method comprising: (a) preparing a lysate sample wherein the preparation of the lysate comprises contacting the sample with: (i) at least one chaotropic agent, preferably a phosphate; (ii) at least one RNA enzyme inhibitor; (iii) at least one protein precipitating agent, and (iv) at least one inhibitor removing agent, (b) clarifying the lysate; (c) contacting the clarified lysate with at least one protein precipitating agent and at least one inhibitor removing agent and providing a mixture; (d) obtaining an RNA-containing liquid phase from the mixture; and (e) recovering RNA from the liquid phase. The method enables the isolation of high purity, high yield RNA from inhibitor-rich samples, such as soil, feces and wastewater samples. The method provided by the invention can effectively remove inhibitory pollutants.

The invention relates to the technical field of protein engineering and molecular detection, in particular to a fusion protein with DNA polymerase activity, reverse transcriptase activity and RNase inhibitory activity and application thereof. The fusion protein provided by the invention comprises a DNA polymerase functional domain and an RNase inhibitory protein functional domain, the amino acid sequence of the RNase inhibitory protein functional domain is as shown in SEQ ID NO: 2. Through an innovative protein fusion strategy, multiple key enzyme activities such as reverse transcription and nucleic acid amplification are integrated into a single protein, and the problems that a traditional multi-enzyme system is poor in compatibility and conditions are difficult to unify are fundamentally solved. The fusion protein shows excellent tolerance to various known PCR inhibitors such as high-concentration chaotropic agents, RNase and heparin, and the reaction stability and the success rate in complex samples are remarkably improved.

Provided is a method for isolating nucleic acids from a plant sample comprising(a) preparing a lysed sample wherein preparing comprises(i) lysing a plant sample by mechanically disrupting the plant sample in a lysis solution which comprises at least one chaotropic agent and one or more solid disrupting particles, and(ii) optionally clearing the lysate;(b) contacting the lysed sample with at least one protein precipitating agent and at least one inhibitor removing agent and providing a mixture;(c) obtaining a liquid phase from the mixture; and(d) isolating nucleic acids from the liquid phase.Also provided is a kit for use in such method.

The invention discloses nanoparticles for efficiently and stably delivering cannabidiol as well as a preparation method and application of the nanoparticles, and belongs to the technical field of bioactive substance delivery. The method is characterized in that a soybean protein structure is induced to generate controllable folding and re-folding by adopting a pH migration method, cannabidiol is efficiently encapsulated by utilizing the hydrophobic effect of protein in the process, and composite nanoparticles are formed through self-assembly. According to the method, when the mass ratio of the soybean protein to the cannabidiol is 10: 1, the encapsulation efficiency can reach 96.01%, the particle size of the obtained nanoparticles is uniform, and the average particle size is smaller than or equal to 100 nm. The stability of the cannabidiol can be effectively improved, the retention rate of the cannabidiol is still larger than or equal to 90% after the cannabidiol is heated in water at 80 DEG C for 2 hours, the antioxidant activity of the cannabidiol is remarkably enhanced, and the in-vitro bioavailability of the cannabidiol is improved. The whole preparation process is mild in condition, an organic solvent or chaotropic agent does not need to be used, safety is achieved, the green production concept is met, and the obtained nanoparticles have wide application prospects in medicine.

The present invention relates to a method for producing an extracellular matrix hydrogel. The method comprising decellularizing a tissue sample to form a decellularized tissue mesh, the decellularizing comprising mixing the tissue sample with a wash solution to form a decellularizing mixture, wherein the tissue sample is derived from a tumor having a fibrosis content ranging from about 40 % to about 90 %, and separating the decellularizing mixture into a cellular waste fluid and a decellularized tissue mesh; depolymerizing the decellularized tissue mesh to form a depolymerized protein dispersion, the depolymerizing comprising mixing the decellularized tissue mesh with a depolymerizing solution comprising a chaotropic agent to form a depolymerizing mixture, mechanically homogenizing the depolymerizing mixture at a chilled temperature to form a homogenized mixture, separating the homogenized mixture into a waste precipitate and a supernatant comprising the depolymerized protein dispersion; filtering the depolymerized protein dispersion to form a sol; and gelling the sol to form the extracellular matrix hydrogel, the gelling comprising combining the sol with gelling solution comprising a kosmotropic agent, and separating a fluid phase away from the extracellular matrix hydrogel as it forms.

The invention discloses a method for detecting related substances of trazodone hydrochloride sustained release tablets, and belongs to the technical field of pharmaceutical analysis. According to the detection method, high performance liquid chromatography is adopted, and the chromatographic condition is that an octadecyl bonded silica gel column is used as a chromatographic column; the column temperature is 25-35 DEG C, and the flow velocity is 0.8-1.2 ml / min; the sample size is 5-15 [mu] l, and the detection wavelength is 252-256 nm; the mobile phase A is a potassium hexafluorophosphate buffer solution; the mobile phase B is acetonitrile; and carrying out gradient elution. Compared with trazodone hydrochloride sustained-release tablet imported drug registration standards and United States Pharmacopoeia related substance detection methods of trazodone hydrochloride bulk drugs, the method abandons a tailing-sweeping agent triethylamine suitable for an alkaline sample, adjusts the pH value of a mobile phase to be acidic, improves the peak shape and increases retention of trazodone hydrochloride and impurities through a chaotropic agent potassium hexafluorophosphate buffer solution, and improves the quality of trazodone hydrochloride sustained-release tablets. The method has the advantages of moderate impurity retention, good separation effect, excellent peak shape, long service life of the chromatographic column and reduction of the analysis cost. The method has the advantages of strong specificity, high sensitivity, high accuracy and good durability.