41 results about "Chaotropic agent" patented technology

The present disclosure provides methods for isolating nucleic acids from a sample, comprising: (a) contacting a sample, a lysate of the sample, a supernatant of the lysate, or a portion of the sample, the lysate or the supernatant with one or more first agents (e.g., protein precipitating agents) and one or more second agents (e.g., inhibitor removing agents) to generate a mixture, (b) separating the mixture of step (a) into a solid phase and a liquid phase, wherein the one or more second agents are primarily in the solid phase, and (c) isolating nucleic acids from the liquid phase of step (b). Compositions and kits useful in such methods are also disclosed. Further disclosed are methods, compositions and kits for preparing a lysate using a lytic reagent comprising one or more relatively mild chaotropic agents and one or more phosphates from a sample, especially a complex sample, such as a soil or stool sample.

A supramolecular histochemical system for staining, the system comprising chaotropic ions and a chaotropic ion complexing agent that accepts chaotropic ions as a molecular subject as a molecular guest. The chaotropic ions facilitate diffusion of probes (e.g., antibodies) into and within the tissue sample, followed by binding or dilution of the guest to the subject to facilitate binding of the antibodies to the target antigen and immunostaining or generation of histochemical signals. Alternatively, the micromolecular fluorescent probe can be used as a molecular guest to be compounded with the supramolecular subject to promote deep penetration of the supramolecular subject. A method for performing histology employs a supramolecular system with deep homogeneous histochemistry. The method is relatively fast, scalable, automated and cost effective.

The invention relates to a unified system for the extraction and purification of biomolecules using a novel Cloudy-Precipitated (CP) Buffer and an integrated extraction device. The CP Solution remains cloudy even when heated and enables single- or multi-step workflows for isolating nucleic acids without requiring multiple buffers or organic solvents. It contains optimized concentrations of detergents, chaotropic agents, salts, and adsorbents to efficiently lyse cells and minimize processing steps. The associated device incorporates a heating and filtration system that allows separation of target molecules from inhibitors or impurities based on molecular weight. It can operate as a single- or double-step configuration with various filter membrane options. This system reduces time, labor, and environmental impact while enhancing sample quality for molecular diagnostics, research, and therapeutic applications.

Protein hydrolysate compositions and methods of making the same are disclosed. The protein hydrolysate composition has a protein-rich organic content. The protein hydrolysate composition may be substantially free of exogenous chelating agents, chaotropic agents and surfactants. The protein hydrolysate composition may be low in ash content. The protein hydrolysate composition is produced by processing a biomass, e.g., a microbial biomass, through a combination of physical, chemical and / or enzymatic treatments. The protein hydrolysate may be sourced via microbial biomass from CCk as a carbon source. Also disclosed are methods of using the protein hydrolysate compositions, e.g., as a biostimulant.

A method of stabilizing a chemokine protein with a C-X-C or a C-C structural motif such as CXCL13 and CCL21 is provided. The method comprises the step of introducing the chemokine proteins into a solution comprising a buffer a non- ionic or amphoteric surfactant; and an aqueous chaotropic agent. A method of detecting a chemokine protein with a C-X-C or a C-C structural motif such as CXCL13 and CCL21 conjugated to antibodies thereto labelled with a fluorescent label in a solution is also provided. The method comprises the following steps: providing a test substrate comprising an elongate nitro cellulose membrane.

The invention relates to the technical field of nucleic acid detection, in particular to a lysis buffer solution, a kit and an extraction method for extracting free DNA of peripheral blood. The lysis buffer solution for extracting the free DNA of the peripheral blood comprises a first lysis solution and a second lysis solution, the first lysis solution takes guanidine hydrochloride as a main chaotropic agent and at least one of ammonium thiocyanate and guanidine isothiocyanate as an auxiliary chaotropic agent, and is compounded with a metal ion chelating agent, trehalose, dithiothreitol, digestive enzyme, sodium dodecyl sarcosinate, borate and ascorbic acid; the second lysis solution takes guanidine hydrochloride as a main chaotropic agent and urea as an auxiliary chaotropic agent, and is compounded with a metal ion chelating agent, trehalose, dithiothreitol, digestive enzyme, sodium dodecyl sarcosinate, borate, ascorbic acid and polyethylene glycol. The buffer solution of the lysate does not contain SDS (sodium dodecyl sulfate), so that the residue of SDS in an extracted product can be avoided, and the amount of extracted free DNA (deoxyribonucleic acid) is more.

The invention discloses a humic acid adsorbent, a kit containing the humic acid adsorbent and an extraction method of soil metagenome DNA. The humic acid adsorbent is prepared from a polypeptide compound, a chaotropic agent and water, wherein in the humic acid adsorbent, the content of the polypeptide compound is 150 to 500g / L, and the content of the chaotropic agent is 0.5 to 5M. According to the humic acid adsorbent and the kit containing the humic acid adsorbent, inhibitors such as humic acid in soil can be efficiently and specifically adsorbed, meanwhile, the adsorption capacity to a nucleic acid macromolecular structure is greatly reduced, and the extracted metagenome DNA of soil microorganisms is good in integrity and high in purity. The method provided by the invention is suitable for extracting the microbial metagenome DNA in soil samples from different regions and different sources, and has broad-spectrum applicability.

The present disclosure provides compositions and methods for sample processing, particularly for oligonucleotide analysis e.g. analysis of formulated nucleic acid drugs. A composition for pretreating at least one target nucleic acid in a biological mixture provided herein includes a chaotropic agent selected from a substituted guanidine, a substituted amidine, a substituted quaternary amine, or a combination thereof, an optional protease, and / or an optional disulfide-reducing agent. Methods of analyzing at least one target nucleic acid in a biological mixture is also provided herein. Furthermore, the present disclosure provides methods for quantifying at least one target cationic lipid interacting with a nucleic acid.

The present disclosure provides a buffer comprising at least one chaotropic agent, at least one chelating agent and at least one non-ionic surfactant, wherein the buffer stabilizes a biological sample at about room temperature for at least about one day.

The invention discloses a method for simultaneously extracting DNA (deoxyribonucleic acid) and RNA (ribonucleic acid), and relates to the technical field of molecular biology, the method comprises the following steps: sample lysis incubation: co-incubating a biological sample with a lysis buffer solution, a reducing agent and proteolytic enzyme; adding a binding solution, namely adding the binding solution into the mixture subjected to lysis incubation, wherein the binding solution at least comprises a chaotropic agent, a nonionic surfactant and alcohol; according to the method, the biological sample is incubated together with the cracking buffer solution which does not contain chaotropic agent and chelating or complexing agent, the reducing agent and the proteolytic enzyme, and the anionic surfactant and the buffer compound in the cracking buffer solution have a synergistic effect and are matched with the reducing agent and the proteolytic enzyme, so that the biological sample can be efficiently cracked without physical crushing treatment; and even complex samples such as fiber tissues and high cell content can be fully cracked, so that DNA and RNA released from the samples are more complete, and nucleic acid loss and breakage caused by insufficient cracking are reduced.

Provided is a method for isolating nucleic acids from a plant sample comprising (a) preparing a lysed sample wherein preparing comprises (i) lysing a plant sample by mechanically disrupting the plant sample in a lysis solution which comprises at least one chaotropic agent and one or more solid disrupting particles, and (ii) optionally clearing the lysate; (b) contacting the lysed sample with at least one protein precipitating agent and at least one inhibitor removing agent and providing a mixture; (c) obtaining a liquid phase from the mixture; and (d) isolating nucleic acids from the liquid phase. Also provided is a kit for use in such method.

The present disclosure provides methods for isolating nucleic acids from a sample, comprising: (a) contacting a sample, a lysate of the sample, a supernatant of the lysate, or a portion of the sample, the lysate or the supernatant with one or more first agents (e.g., protein precipitating agents) and one or more second agents (e.g., inhibitor removing agents) to generate a mixture, (b) separating the mixture of step (a) into a solid phase and a liquid phase, wherein the one or more second agents are primarily in the solid phase, and (c) isolating nucleic acids from the liquid phase of step (b). Compositions and kits useful in such methods are also disclosed. Further disclosed are methods, compositions and kits for preparing a lysate using a lytic reagent comprising one or more relatively mild chaotropic agents and one or more phosphates from a sample, especially a complex sample, such as a soil or stool sample.

The present invention relates to methods for purifying Fc-containing proteins. The present invention relates to methods for purifying proteins containing an Fc region, such as antibodies and Fc fusion proteins. In particular, the present invention relates to a purification method resulting in reduced levels of aggregate proteins, the method comprising adsorbing a protein containing an Fc region to a temperature-responsive protein A resin, and eluting the protein from the resin at a temperature below about 35C with an elution buffer comprising a chaotropic agent, a sugar alcohol, and at least one amino acid. Also described is a method for separating fully assembled antibodies from their half-antibody forms using an elution buffer.

The invention relates to the technical field of plasma free DNA extraction, and particularly discloses a lysis binding solution, a kit and an extraction method for plasma free DNA extraction. The cracking binding liquid comprises the following components: chaotropic agent, chelating agent, buffering agent, defoaming agent and detergent; wherein the content of the defoaming agent in the cracking combination liquid is 0.0005 to 0.01 weight percent; the content of the detergent in the cracking combination liquid is 1 to 10 weight percent. The extraction kit comprises the lysis binding solution. In the extraction method, the cracking binding liquid is used. The synergistic effect of a defoaming agent and a detergent in the lysis binding liquid remarkably improves the extraction efficiency of free DNA in plasma, and later nucleic acid detection is facilitated.

The invention provides a modified dolomite powder, a preparation method thereof, and a concrete. The modified dolomite powder is prepared from 98% to 99% of a dolomite powder, 0.2% to 0.5% of a chaotropic agent, 0.6% to 1.0% of a dihydrogen phosphate, and 0.2% to 0.5% of a capillary filler according to the mass percentage. The chaotropic agent is one or more of sodium sulfate, potassium sulfate, and ammonium sulfate. By promoting the dissolution of the surface of the dolomite powder, participating in the hydration reaction, and filling capillary pores, the triple modification solves the problems of bleeding, strength, and durability of the dolomite powder concrete in the related art. The obtained modified dolomite powder has good solubility and high chemical activity, and the prepared concrete has high strength and compactness, low porosity, and good durability.

The invention relates to a unified system for the extraction and purification of biomolecules using a novel Cloudy-Precipitated (CP) Buffer and an integrated extraction device. The CP Solution remains cloudy even when heated and enables single- or multi-step workflows for isolating nucleic acids without requiring multiple buffers or organic solvents. It contains optimized concentrations of detergents, chaotropic agents, salts, and adsorbents to efficiently lyse cells and minimize processing steps. The associated device incorporates a heating and filtration system that allows separation of target molecules from inhibitors or impurities based on molecular weight. It can operate as a single- or double-step configuration with various filter membrane options. This system reduces time, labor, and environmental impact while enhancing sample quality for molecular diagnostics, research, and therapeutic applications.

The present disclosure relates to a method for producing an extracellular matrix hydrogel, the method comprising (a) decellularizing a tissue sample to form a decellularized tissue mesh; (b) depolymerizing the decellularized tissue mesh to form a depolymerized protein dispersion, the depolymerizing comprising mixing the decellularized tissue mesh with a depolymerizing solution comprising a chaotropic agent to form a depolymerizing mixture; mechanically homogenizing the depolymerizing mixture at a chilled temperature to form a homogenized mixture; separating the homogenized mixture into (a) a waste precipitate and (b) a supernatant comprising the depolymerized protein dispersion; (c) filtering the depolymerized protein dispersion to form a sol; and (d) gelling the sol to form the extracellular matrix hydrogel, the gelling comprising combining the sol with a gelling solution comprising a kosmotropic agent; and separating a fluid phase away from the extracellular matrix hydrogel as it forms.

The present invention provides an improved manufacturing process for producing a bispecific antibody product comprising at least two binding domains. The process comprises at least a process comprising a step of refolding a construct, wherein one or more domains comprise disulfide bonds, and wherein the construct is contacted with a refolding buffer to refold the construct into its native form, the buffer comprising (i) an oxidizing and / or reducing agent at a concentration of at least 0.1 mM, and (ii) a chaotropic agent at a concentration > 1 M, and wherein the pH of the refolding buffer corresponds to + / - 4.5 of the pi value of the first domain or + / - 4.0 of the pi value of the entire construct.

The invention relates to the technical field of cosmetics, and discloses a silk-core protein fiber aggregate, a silk-core protein carrier and a preparation method and application thereof.The preparation method of the silk-core protein fiber aggregate comprises the steps of mixing, specifically, an extract obtained after degumming of silk and a chaotropic agent aqueous solution are mixed according to the volume ratio, and a silk extract aqueous solution is obtained; a dissolving step: placing the silk extract aqueous solution in a constant-temperature water bath, and continuously magnetically stirring until the silk fibroin is completely dissolved to obtain a dissolved silk fibroin solution; and a cultivation step: carrying out concentration-dilution-temperature cultivation treatment on the silk fibroin solution to obtain the silk fibroin fiber aggregate containing the beta-folders. According to the technical scheme provided by the invention, the problem that an existing liposome carrier has defects in the aspects of stability and irritation can be solved.

A formulation comprising an isothiocyanate (ITC) with a sulfoxide moiety and a combination of at least two of the following compounds: - organic acids, - amino acids, - ectoine and 5-hydroxyectoine, - compounds comprising a tetramethyl ammonium moiety, - phenols, - glycols, - glycerol, - sugar alcohols, - simple saccharides, - terpenoids, - chaotropic agents, wherein the above-mentioned compounds may be mixed in any combination with the selected isothiocyanate. Products comprising said formulation. A method of preparing said formulation. A method of stabilizing isothiocyanate in said formulation.

Provided is a method for isolating nucleic acids from a plant sample comprising(a) preparing a lysed sample wherein preparing comprises(i) lysing a plant sample by mechanically disrupting the plant sample in a lysis solution which comprises at least one chaotropic agent and one or more solid disrupting particles, and(ii) optionally clearing the lysate;(b) contacting the lysed sample with at least one protein precipitating agent and at least one inhibitor removing agent and providing a mixture;(c) obtaining a liquid phase from the mixture; and(d) isolating nucleic acids from the liquid phase.Also provided is a kit for use in such method.

The invention relates to the technical field of biological peptides, and discloses a weight-losing peptide with high activity as well as a preparation method and application thereof, and the weight-losing peptide is prepared from an Arg34-GLP-1 (7-37) main chain, an acylating agent, an N-acetyl-L-arginine chaotropic regulator, a sodium benzoate interface regulator, a zinc salt masking agent and a reaction solvent. The preparation method comprises the following steps: carrying out in-situ coordination masking on the N-terminal of polypeptide by using zinc ions in a water phase, then feeding a reaction solvent to implement solvent polarity switching so as to construct a homogeneous reaction system, then carrying out high-selectivity acylation reaction, and finally carrying out metal decomplexation and deprotection treatment. According to the invention, the technical problems of serious N-terminal competitive side reaction, easy racemization of histidine and easy aggregation and precipitation of polypeptide in a mixed solvent in the modification process are effectively solved by utilizing the synergistic effect of metal coordination locking and a chaotropic regulator, and the obtained product is high in regioselectivity and complete in biological activity.

Disclosed herein are novel buffers for rapid and simplified nucleic acid extraction, and methods of their use. The buffers comprise a chaotropic agent, such as guanidine hydrochloride, sodium chloride, or lithium chloride, combined with Tris-EDTA buffer at a specific pH. The buffers facilitate efficient lysis of cells and viruses, inactivate nucleases, and enable binding of nucleic acids to various silica surfaces without the need for multiple wash steps or specialized equipment.

The invention belongs to the technical field of medicines, and particularly relates to application of combination of gelatin and chaotropic agent in preparation of a medicine for treating androgenetic alopecia. The invention provides a chaotropic agent-gelatin collaborative engineering strategy, and a protein microneedle composed of gelatin and a chaotropic agent is constructed. Through the screening preparation method, the protein microneedle has high mechanical properties and high skin puncture efficiency. When the protein microneedle is used for treating androgenetic alopecia, compared with a single NaI or gelatin protein microneedle, the protein microneedle has a synergistic effect in the aspects of improving new hair, improving the number of hair follicles and promoting hair follicles in a resting period to be converted into a growing period, and the effect of the protein microneedle is superior to that of minoxidil medicine; the combination of the gelatin and chaotropic agent has a good application prospect in preparation of the medicine for treating androgenetic alopecia.

The present invention discloses a novel water treatment technology, specifically a nanofiltration membrane made by pre-reaction of trace monomers. The present invention is to mix a low-carbon chain alkane solvent and a high-carbon chain alkane to form a mixed organic phase solvent; then dissolve a polyacyl chloride monomer into the mixed organic phase solvent to prepare an organic solution A; then dissolve a trace reaction monomer and a chaotropic agent into another portion of the same volume of mixed organic phase solvent to prepare an organic solution B; cool, mix, and heat the organic solution A and the organic solution B respectively to prepare an organic solution C; further dissolve a polyamine monomer in water to prepare an aqueous phase solution; finally, place an ultrafiltration base membrane into the aqueous phase solution and the organic solution C, and then dry it. The advantage of the present invention is that it can effectively change the charge and hydrophilicity and hydrophobicity properties in the nanofiltration membrane structure, achieve precise control of the nanofiltration membrane performance in a wide range, and is stable, reliable, and easy to implement.

A method for producing a natural formaldehyde-free adhesive for wood panels, which improves the crosslinking reactions between the crosslinking agent and the binding agent, comprising reacting a plant protein selected from soy, lupine and legumes, together with a crosslinking agent selected from 100% of an oxidized saccharide or a combination of an oxidized saccharide and an unoxidized or pure saccharide; the method comprises the steps of: an oxidation step of a saccharide; a preparation step of plant protein, in which the plant protein is reacted with a chaotropic agent or alkali or by temperature control or a combination of these conditions; and a mixing step.

The invention relates to the technical field of biological medicines, in particular to an eluent and application thereof in purification of long-chain RNA (Ribonucleic Acid). The eluent is formed by mixing an acidic buffer solution 1 with the pH value of 3.0-6.6 and a monobasic strong base solution 2 with the concentration of 0.2-4M in proportion. Experiments show that the eluent provided by the invention realizes anion exchange chromatography purification of large-fragment RNA (greater than 300nt) in a normal temperature / room temperature environment (10-37 DEG C). A chaotropic agent (such as urea) is added into a chromatographic system before the eluent is used for elution, or the chaotropic agent is further added into the eluent for elution, the purification method provided by the invention is stable and easy to amplify, and a foundation is laid for subsequent large-scale RNA drug development.

A reagent combination for extracting free DNA in plasma, a kit comprising the reagent combination, and a method for extracting free DNA in plasma by using the reagent combination or the kit. The reagent combination comprises a binding solution and a first washing solution, wherein the binding solution comprises a first chaotropic agent and a first alcohol, and the first washing solution comprises a second chaotropic agent and a second alcohol. The present invention is suitable for large-scale clinical detection, and has a relatively high DNA yield and DNA product purity.

The present invention provides a two-piece assembly for continuous treatment of solutions and / or solids through a substrate, the assembly having an inner vial holding and containing the substrate and an outer vial configured to receive the inner vial in an upper or lower parking position, to allow or prevent the solution from passing through the substrate of the upper vial, respectively. The captured molecules can be treated in situ in the matrix with enzymes and / or chemicals without the need for the use of strong chaotropic agents such as urea or detergents such as SDS.

The invention relates to a unified system for the extraction and purification of biomolecules using a novel Cloudy-Precipitated (CP) Buffer and an integrated extraction device. The CP Solution remains cloudy even when heated and enables single- or multi-step workflows for isolating nucleic acids without requiring multiple buffers or organic solvents. It contains optimized concentrations of detergents, chaotropic agents, salts, and adsorbents to efficiently lyse cells and minimize processing steps. The associated device incorporates a heating and filtration system that allows separation of target molecules from inhibitors or impurities based on molecular weight. It can operate as a single- or double-step configuration with various filter membrane options. This system reduces time, labor, and environmental impact while enhancing sample quality for molecular diagnostics, research, and therapeutic applications.