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3 results about "Chaotropic agent" patented technology
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A chaotropic agent is a molecule in water solution that can disrupt the hydrogen bonding network between water molecules (i.e. exerts chaotropic activity). This has an effect on the stability of the native state of other molecules in the solution, mainly macromolecules (proteins, nucleic acids) by weakening the hydrophobic effect. For example, a chaotropic agent reduces the amount of order in the structure of a protein formed by water molecules, both in the bulk and the hydration shells around hydrophobic amino acids, and may cause its denaturation.
Downstream processing of bispecific antibody constructs
ActiveCN112789058BSerum immunoglobulinsPeptide preparation methodsDisulfide bondingDownstream processing
The present invention provides an improved manufacturing process for producing a bispecific antibody product comprising at least two binding domains. The process comprises at least a process comprising a step of refolding a construct, wherein one or more domains comprise disulfide bonds, and wherein the construct is contacted with a refolding buffer to refold the construct into its native form, the buffer comprising (i) an oxidizing and / or reducing agent at a concentration of at least 0.1 mM, and (ii) a chaotropic agent at a concentration > 1 M, and wherein the pH of the refolding buffer corresponds to + / - 4.5 of the pi value of the first domain or + / - 4.0 of the pi value of the entire construct.
Owner:AMGEN INC
Rapid nucleic acid extraction from biofluids
Disclosed herein are novel buffers for rapid and simplified nucleic acid extraction, and methods of their use. The buffers comprise a chaotropic agent, such as guanidine hydrochloride, sodium chloride, or lithium chloride, combined with Tris-EDTA buffer at a specific pH. The buffers facilitate efficient lysis of cells and viruses, inactivate nucleases, and enable binding of nucleic acids to various silica surfaces without the need for multiple wash steps or specialized equipment.
Owner:THE ARIZONA BOARD OF REGENTS ON BEHALF OF THE UNIV OF ARIZONA
A chaotropic agent-triggered fast absorbable protein microneedle, an anesthetic protein microneedle and a preparation method and use thereof
PendingCN122097234AOrganic active ingredientsInorganic non-active ingredientsGelatinLocal anesthesia
The application belongs to the technical field of medicine, and particularly relates to a chaotropic agent triggered fast absorbable protein microneedle, an anesthetic protein microneedle and a preparation method and application thereof. Iodine ions are selected as typical chaotropic agents, and gelatin is selected as a protein, and a new protein microneedle of interaction between the chaotropic agent and the protein is constructed through a two-step method. The protein microneedle has the characteristics of rheology, high mechanical strength and in-vivo fast absorption, and realizes the balance of 'processability, high mechanical strength and fast dissolution behavior'. The chaotropic agent triggered fast absorbable protein microneedle is applied to the fields of immunoregulation and local anesthesia respectively, and the system realizes the transition of the microneedle from a 'passive carrier' to an 'active therapeutic agent', and has a good application prospect.
Owner:SICHUAN UNIV