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Troponin I latex-enhanced immunological turbidimetry detection kit

A detection kit, troponin technology, used in immunology and in vitro diagnosis, medical field

Active Publication Date: 2018-09-14
BEIJING STRONG BIOTECH INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, most of the detection reagents used in clinical practice have not taken measures to prevent RF interference (Research progress on the interference of rheumatoid factors on immunoassays, Medical Review 2015 Vol 21, 19: 3495)

Method used

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  • Troponin I latex-enhanced immunological turbidimetry detection kit
  • Troponin I latex-enhanced immunological turbidimetry detection kit
  • Troponin I latex-enhanced immunological turbidimetry detection kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Embodiment 1: the preparation of the kit of the present application

[0046] 1. The preparation process of the first reagent is as follows:

[0047] The first reagent contains

[0048]

[0049] At room temperature, the preparation of the first reagent was carried out as described above, and the blocking agent was purified mouse anti-human IgM monoclonal antibody (purchased from Scantibodies Laboratory Inc. 9336 Abraham Way, Santee, 92071, California, USA, Cat. No. 3KC542; the purity was greater than 95 %).

[0050] 2. The preparation process of the second reagent is as follows:

[0051] Add 0.5ml of polystyrene latex solution (concentration 10%) with a diameter of 300nm to 4.5ml 0.05M MES (PH6.0) and 100μl EDC (carbodiimide, concentration 5mg / mL), and stand at 37°C for 1 hour;

[0052] Troponin I monoclonal antibody was diluted with 5ml 0.05M Tris buffer (pH7.5) and immediately added to the above buffer, and reacted at 37°C for 2 hours;

[0053] Add 1ml 0.1M glycin...

Embodiment 2

[0061] Embodiment 2: the preparation of control kit

[0062] The preparation method is the same as that in Example 1, the only difference being that the monoclonal antibody is replaced by a polyclonal antibody.

Embodiment 3

[0063] Example 3. Comparison of Surfactants

[0064] Prepare the kit according to the method of Example 1, the only difference is: replace the Tween 80 in the first reagent with various surfactants of different concentrations in the table below; and test the standards of different concentrations respectively, the results are as follows.

[0065] Table 1. Comparison of Surfactants

[0066]

[0067]

[0068] The data of S1-S6 is the calibration reaction absorbance, the larger the reaction gradient (ie slope), the lower the blank reaction absorbance (ie S1 reaction absorbance), the better. It can be seen from the results that the effect is the best when Tween 80 is 10g / L, followed by 20g / L Tween80.

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Abstract

The invention relates to a troponin I latex-enhanced immunological turbidimetry detection kit which particularly comprises a first reagent and a second reagent. The first reagent comprises buffer solution, blocking agents, surface active agents, electrolyte, stabilizers, polyethylene glycol, chaotropic agents and preservatives; the second reagent comprises latex particles coated with human troponin I monoclonal antibodies, buffer solution, surface active agents, stabilizers and preservatives. The kit displays better interference resistance.

Description

technical field [0001] The disclosure relates to the fields of medicine, immunity and in vitro diagnosis, and specifically relates to a kit for detecting troponin I by immunoturbidimetry. Background technique [0002] Cardiac troponin I (cTn I) is one of the three subunits of cardiac troponin (cTn), the other two subunits are cardiac troponin T (cTnT) and troponin C (TnC). cTnI is composed of approximately 209 amino acid residues, with a molecular weight of 23.9kD and an isoelectric point of 9.87. It is a protein rich in α-helices. Only 3-6% of cTnI exists in the cytoplasm in free form, and most of it exists in the form of complexes on thin myofilaments. After myocardial injury, the cardiac troponin complex is released into the blood, and after 4-6 hours, it begins to increase in the blood, and the elevated troponin I can remain in the blood for 6-10 days. [0003] Of these proteins involved in myocyte contraction, troponin I is highly cardiac-specific and sensitive, and i...

Claims

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Application Information

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IPC IPC(8): G01N33/68G01N33/577
CPCG01N33/577G01N33/6893G01N2333/47G01N2800/325
Inventor 程红党刘瑶刘希
Owner BEIJING STRONG BIOTECH INC
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