4 results about "Troponin I" patented technology

The invention relates to the field of antibodies, in particular to an anti-troponin I monoclonal antibody and application thereof, performance analysis of a purified electrophoretogram of the antibody and a kit coupled with a monoclonal antibody HK401 coated magnetic particle component shows that the monoclonal antibody has excellent performance in cTni detection, and the cTni detection sensitivity is high. The method can be used as an object for further production and optimization.

This invention discloses a highly sensitive method for detecting troponin I. This method utilizes the reusable nature of a biosensor to perform multiple fluorescence signal reads. Based on these multiple reads, it selects the most accurate concentration range for determining concentrations in the low-to-medium concentration range, achieving higher resolution detection results in both low and medium concentration areas. Furthermore, it incorporates chemiluminescence detection, employing higher-resolution chemiluminescence to determine the final concentration in the high-concentration region. Ultimately, this method achieves better resolution detection results across high, medium, and low concentration regions, enabling accurate identification of sample concentrations across all concentration ranges. It boasts a sensitivity of 0.824 ng / L and a detection range of 0.824 ng / L–100,000 ng / L. The method significantly reduces sample volume, requiring only 20 μL for accurate detection, thus reducing blood volume and improving blood collection efficiency in physical examinations.

The application discloses an antibody for detecting cardiovascular diseases and application thereof, and belongs to the technical field of biological medicine. The monoclonal antibody or antigen binding fragment thereof provided by the application can specifically bind to a specific antigen epitope (SEQ ID NO:1) of human cardiac troponin I (cTnI) and has no cross reaction with skeletal muscle troponin I. The application further discloses the CDR sequences of the heavy chain and light chain variable regions of the antibody, a coding gene, a preparation method and application thereof. An ELISA detection kit containing the antibody can specifically recognize cTnI in a blood sample, has good linearity in the range of 0.01-50 ng / mL, has a false negative rate of 0 for serum detection of patients with acute myocardial infarction, and has a low false positive rate for serum detection of healthy people. The antibody and the kit have important application value in rapid and accurate diagnosis of cardiovascular diseases such as acute myocardial infarction.

The invention discloses a method for detecting high-sensitivity troponin I. According to the method, fluorescence signals are read for multiple times by utilizing the characteristic that a biosensor can be moved for multiple times, and how to more accurately determine the concentration in a medium-concentration interval and a low-concentration interval is selected according to the fluorescence signals read for multiple times. A detection result with a higher separation degree can be obtained in a low-concentration region and a medium-concentration region, chemiluminescence detection is further combined, the final concentration is determined by adopting chemiluminescence with a higher separation degree in a high-concentration region, and finally the detection result with a better separation degree can be obtained in the high-concentration region, the medium-concentration region and the low-concentration region. Therefore, the method provided by the invention can accurately identify the concentrations of the samples with different concentrations in each concentration region. The method has the advantages that the sensitivity reaches 0.824 ng / L, and the detection range is between 0.824 ng / L and 100000 ng / L. According to the method disclosed by the invention, the sample dosage is greatly reduced, accurate detection can be realized only by 20 microliters, the blood sampling amount is favorably reduced, and the blood sampling physical examination is improved.