Patents
Literature
2 results about "Troponin I" patented technology
Efficacy Topic
Property
Owner
Technical Advancement
Application Domain
Technology Topic
Technology Field Word
Patent Country/Region
Patent Type
Patent Status
Application Year
Inventor
Troponin I is a cardiac and skeletal muscle protein family. It is a part of the troponin protein complex, where it binds to actin in thin myofilaments to hold the actin-tropomyosin complex in place. Because of it, myosin cannot bind actin in relaxed muscle. When calcium binds to the troponin C it causes conformational changes which lead to dislocation of troponin I and finally tropomyosin leaves the binding site for myosin on actin leading to contraction of muscle. The letter I is given due to its inhibitory character. It is a useful marker in the laboratory diagnosis of heart attack. It occurs in different plasma concentration but the same circumstances as troponin T - either test can be performed for confirmation of cardiac muscle damage and laboratories usually offer one test or the other.
High sensitivity troponin i detection method
This invention discloses a highly sensitive method for detecting troponin I. This method utilizes the reusable nature of a biosensor to perform multiple fluorescence signal reads. Based on these multiple reads, it selects the most accurate concentration range for determining concentrations in the low-to-medium concentration range, achieving higher resolution detection results in both low and medium concentration areas. Furthermore, it incorporates chemiluminescence detection, employing higher-resolution chemiluminescence to determine the final concentration in the high-concentration region. Ultimately, this method achieves better resolution detection results across high, medium, and low concentration regions, enabling accurate identification of sample concentrations across all concentration ranges. It boasts a sensitivity of 0.824 ng / L and a detection range of 0.824 ng / L–100,000 ng / L. The method significantly reduces sample volume, requiring only 20 μL for accurate detection, thus reducing blood volume and improving blood collection efficiency in physical examinations.
Owner:星童医疗技术(苏州)有限公司
An antibody for detecting cardiovascular disease and use thereof
The application discloses an antibody for detecting cardiovascular diseases and application thereof, and belongs to the technical field of biological medicine. The monoclonal antibody or antigen binding fragment thereof provided by the application can specifically bind to a specific antigen epitope (SEQ ID NO:1) of human cardiac troponin I (cTnI) and has no cross reaction with skeletal muscle troponin I. The application further discloses the CDR sequences of the heavy chain and light chain variable regions of the antibody, a coding gene, a preparation method and application thereof. An ELISA detection kit containing the antibody can specifically recognize cTnI in a blood sample, has good linearity in the range of 0.01-50 ng / mL, has a false negative rate of 0 for serum detection of patients with acute myocardial infarction, and has a low false positive rate for serum detection of healthy people. The antibody and the kit have important application value in rapid and accurate diagnosis of cardiovascular diseases such as acute myocardial infarction.
Owner:THE THIRD AFFILIATED HOSPITAL OF GUANGZHOU MEDICAL UNIVERSITY (GUANGZHOU SEVERE MATERNAL TREATMENT CENTER GUANGZHOU ROUJI HOSPITAL)