8 results about "Troponin I" patented technology

The invention relates to the field of antibodies, in particular to an anti-troponin I monoclonal antibody and application thereof, performance analysis of a purified electrophoretogram of the antibody and a kit coupled with a monoclonal antibody HK401 coated magnetic particle component shows that the monoclonal antibody has excellent performance in cTni detection, and the cTni detection sensitivity is high. The method can be used as an object for further production and optimization.

The invention relates to the field of intelligent medical treatment, in particular to a pregnancy disease risk prediction method and device based on biochemical indexes and a program product. Comprising the following steps: S1, acquiring serum biochemical index data of a pregnant woman; s2, comparing the serum biochemical indexes with a preset threshold value; the serum biochemical indexes include aspartate aminotransferase, total protein, albumin, lactic dehydrogenase, alkaline phosphatase, total bile acid, creatine kinase, blood sugar, urea nitrogen, uric acid, creatinine, glomerular filtration rate, potassium, chlorine, total calcium, serum magnesium, inorganic phosphorus, total cholesterol, triglyceride, high-density lipoprotein cholesterol, low-density lipoprotein cholesterol and homocysteine. When one or more of the myoglobin, the hypersensitive troponin I, the prealbumin, the cystatin C and the creatine kinase isoenzyme MB are abnormal, judging that the pregnancy eclampsia exists. The method is simple, convenient and rapid to operate and low in cost, can be clinically popularized to predict the health state of the pregnant woman on a large scale, and has good clinical value.

The invention relates to a spatial proximity chemiluminescence kit for detecting troponin I and a preparation method thereof, and relates to the technical field of biology. The kit comprises an enzyme marker, a luminous marker, a luminous auxiliary agent and a triggering agent, the enzyme marker comprises an anti-cTnI monoclonal antibody marked by horse radish peroxidase, and the luminous marker comprises an anti-cTnI monoclonal antibody marked by 9, 10-dihydroacridine. The kit adopts a space adjacent chemical double-antibody sandwich method, is simple to operate, can realize full-automatic detection, does not need magnetic beads and washing, reduces the reagent cost, shortens the detection time, adopts a homogeneous reaction, and has the advantages of high detection accuracy, high sensitivity, high sensitivity and the like by being compared with other methods such as enzyme-linked immunosorbent assay and fluorescence immunochromatography. The influence caused by the traditional chemiluminescence magnetic separation technology is avoided, so that the detection result is more accurate and stable.

This invention discloses a highly sensitive method for detecting troponin I. This method utilizes the reusable nature of a biosensor to perform multiple fluorescence signal reads. Based on these multiple reads, it selects the most accurate concentration range for determining concentrations in the low-to-medium concentration range, achieving higher resolution detection results in both low and medium concentration areas. Furthermore, it incorporates chemiluminescence detection, employing higher-resolution chemiluminescence to determine the final concentration in the high-concentration region. Ultimately, this method achieves better resolution detection results across high, medium, and low concentration regions, enabling accurate identification of sample concentrations across all concentration ranges. It boasts a sensitivity of 0.824 ng / L and a detection range of 0.824 ng / L–100,000 ng / L. The method significantly reduces sample volume, requiring only 20 μL for accurate detection, thus reducing blood volume and improving blood collection efficiency in physical examinations.

The application discloses an antibody for detecting cardiovascular diseases and application thereof, and belongs to the technical field of biological medicine. The monoclonal antibody or antigen binding fragment thereof provided by the application can specifically bind to a specific antigen epitope (SEQ ID NO:1) of human cardiac troponin I (cTnI) and has no cross reaction with skeletal muscle troponin I. The application further discloses the CDR sequences of the heavy chain and light chain variable regions of the antibody, a coding gene, a preparation method and application thereof. An ELISA detection kit containing the antibody can specifically recognize cTnI in a blood sample, has good linearity in the range of 0.01-50 ng / mL, has a false negative rate of 0 for serum detection of patients with acute myocardial infarction, and has a low false positive rate for serum detection of healthy people. The antibody and the kit have important application value in rapid and accurate diagnosis of cardiovascular diseases such as acute myocardial infarction.

The invention discloses a method for detecting high-sensitivity troponin I. According to the method, fluorescence signals are read for multiple times by utilizing the characteristic that a biosensor can be moved for multiple times, and how to more accurately determine the concentration in a medium-concentration interval and a low-concentration interval is selected according to the fluorescence signals read for multiple times. A detection result with a higher separation degree can be obtained in a low-concentration region and a medium-concentration region, chemiluminescence detection is further combined, the final concentration is determined by adopting chemiluminescence with a higher separation degree in a high-concentration region, and finally the detection result with a better separation degree can be obtained in the high-concentration region, the medium-concentration region and the low-concentration region. Therefore, the method provided by the invention can accurately identify the concentrations of the samples with different concentrations in each concentration region. The method has the advantages that the sensitivity reaches 0.824 ng / L, and the detection range is between 0.824 ng / L and 100000 ng / L. According to the method disclosed by the invention, the sample dosage is greatly reduced, accurate detection can be realized only by 20 microliters, the blood sampling amount is favorably reduced, and the blood sampling physical examination is improved.

An assay cartridge enabling improved measurement sensitivity of a target component (Troponin I or T) in the presence of high amount of albumin in a test sample. The assay cartridge includes a cartridge body including a sample inlet adapted to receive a patient sample and a reaction chamber coupled to the sample inlet, magnetic particles contained in the reaction chamber comprising a first antibody with a specific affinity towards the target component, electrodes provided at a location configured to be in contact with a rehydrated test sample containing the magnetic particles in the reaction chamber, and a sensor surface of the reaction chamber comprising a second antibody with a specific affinity towards the target component. Methods of quantifying a target component (e.g., a Troponin component) in immunoassay testing are provided, as are assay devices adapted to quantify a target component (e.g., Troponin I or T).

The invention relates to a lateral flow device for the detection of at least one protein (AAD- protein) released during an event leading to an acute aortic syndrome / acute aortic dissection (AAS / AAD) in a sample of bodily fluid of a human / patient, the device comprising a strip, a sample pad for receiving the sample of the bodily fluid, a first conjugate pad with a conjugate comprising a protein antibody binding to said AAD-protein and a first detection band comprising a first conjugate-binding protein so that the presence of said AAD-protein in the sample is indicated by a visible color change, wherein the AAD-protein is selected from a group of biomarkers for AAS / AAD, preferably HMGI-C or D-Dimer. The lateral flow device further comprises a MI-protein antibody binding to a protein indicative for acute myocardial infarction (MI-protein) wherein the MI-protein is troponin I. In addition, the invention relates in other aspects also to a method for the detection of an AAD-protein in the blood of a patient using the lateral flow device according to the invention, a kit comprising this device as well as a method for determination of the presence of AAD in a patient using an immunoassay.