149 results about "Troponin I" patented technology

A diagnostic tool is disclosed for accurately and rapidly diagnosing the condition of an ailing organ. Although applicable to numerous organ and organ systems, this application particularly illustrates the concept of conjunctive marker utilization as it relates to diagnosing and distinguishing congestive heart failure. The invention particularly relates to the conjunctive utilization of cardiac Troponin I (cTn-I) and natriuretic peptide, e.g. ANP, pro-ANP, BNP, pro-BNP and CNP as a retrospective tool for diagnosing the underlying mechanism of heart failure and as a prospective analytical device for monitoring disease progression and efficacy of therapeutic agents.

The invention is directed to novel combinations of muscle-specific enhancers and promoter elements useful for achieving persistent expression in the muscle or myocyctes. The muscle-specific promoter elements are derived from a muscle creatine kinase promoter, a troponin I promoter, a skeletal alpha-actin promoter, or a desmin promoter. The muscle-specific enhancer elements are derived from either troponin I internal regulatory elements, muscle creatine kinase enhancers, or desmin enhancers.

Antibodies and methods are described for the detection and quantitation of cardiac specific troponin I in samples. Cardiac-specific troponin isoforms exist in various forms in the blood, including free and complexed forms. By selecting antibodies that are insensitive and / or sensitive to these various forms, the present invention can provide immunoassays that more accurately reflect the clinical state of an individual. These described antibodies and methods can be used for providing indicators of myocardial infarction and other cardiac pathologies.

The invention provides methods, compositions, kits, and systems for the sensitive detection of cardiac troponin. Such methods, compositions, kits, and systems are useful in diagnosis, prognosis, and determination of methods of treatment in conditions that involve release of cardiac troponin.

The invention relates to a reagent kit and the preparation method thereof which are used to detect cardiac muscle troponin I; the reagent kit provided by the invention comprises a reagent strip; the reagent strip contains cardiac muscle troponin I monoclonal antibody labeled by colloid gold, unlabeled cardiac muscle troponin I monoclonal antibody and the second antibody of anti-myocardial troponin I monoclonal antibody; the invention also provides the preparation method of the reagent kit of detection. The reagent kit of detection can detect cardiac muscle troponin I in blood sample drawn from human body swiftly, conveniently and delicately; the cardiac muscle troponin I can be used to diagnose myocardial infarction.

Assay systems and specialized antibodies for the detection and quantitation of troponin I and troponin T in body fluids as an indicator of myocardial infarction. Since troponin I and T exist in various conformations in the blood, the ratios of the monomeric troponin I an T and the binary and ternary complexes, as well as which form of troponin present in the blood, may be related to the metabolic state of the heart. Disclosed is a system to determine the presence of a troponin form or a group of troponin forms in a sample of whole blood, serum or plasma.Disclosed is a stabilized composition of troponin; the stabilized composition can comprise a stabilized composition of troponin I, wherein the troponin I is oxidized, the troponin I can be unbound or the troponin I can be in a complex.Disclosed is a method for improving the recovery of troponin I or T from a surface used in immunoassays.Also disclosed are antibodies which recognize, unbound troponin forms, the forms of troponin in binary complexes, the ternary complex of troponin I, T and C, and the conformations of troponin I having intramolecularly oxidized and reduced cysteines.

The invention relates to an anti-human cardiac troponin I hybridoma cell line, with the preservation number of CGMCCNO. 3951, and a monoclonal antibody secreted from the anti-human cardiac troponin I hybridoma cell line CGMCC3951. The class and subclass of immunoglobulin of the monoclonal antibody are respectively IgG and IgG3 and the monoclonal body is specifically combined with human cardiac troponin I; the potency is 1:16000 and the affinity constant is 1.08x10 to 9mol / L. By applying the monoclonal antibody of the invention to clinical diagnosis, the study on complete localization of diagnosis kit for cardiac troponin I is tremendously promoted, correctness of clinical diagnosis and prognosis for cardiovascular diseases is enhanced while suffering of patients and death rate are both reduced, thus prominent economical and social benefits are obtained.

The invention provides methods, compositions, kits, and systems for the sensitive detection of cardiac troponin, Such methods, compositions, kits, and systems are useful in diagnosis, prognosis, and determination of methods of treatment in conditions that involve release of cardiac troponin.

The invention relates to the field of detection in clinical immunology, in particular to a fluorescent immune chromatographic test strip for quantitively detecting troponin I and a preparation method thereof. The test strip provided by the invention comprises a bottom plate, sample absorbing pads attached to the bottom plate and staggered to each other sequentially, a labeled antibody pad, a coating film and a water absorbing pad. The labeled antibody pad is a troponin monoclonal antibody pad I coated with a fluorescent micro-spherical label and a troponin monoclonal antibody pad II coated with a biotin label for identifying different epitopes. The coating film is provided with a detection strip and a quality control strip. Avidin is fixedly arranged on the detection strip. A rabbit antimouse IgG antibody is fixedly arranged on a quality control line. By improving the test strip, a biotin-avidin amplifying system and a fluorescent immune chromatographic technology are introduced into detection of troponin I. Combined with a fluorescent detector, individual quantitive detection of troponin I is realized, therefore, extreme convenience is provided for clinical use.

The invention provides a turbidimetric rapid detection kit for myocardial infarction nano-immunoenhancement. The turbidimetric rapid detection kit comprises a reaction-detection integrated device, and a detection kit arranged in the reaction device, wherein a reagent R1, a reagent R2 and a calibrator are contained in the detection kit; simultaneously, quantitative detection for five important myocardial infarction-related biomarkers in one sample can be realized, and the five important biomarkers include content of troponin-I, content of D-dimer, content of myoglobin, content of hypersensitive C-reactive protein (hs-CRP) and content of heart-type fatty acid binding protein (FABP); and the turbidimetric rapid detection kit has an important clinical significance in the aspects of early diagnosis and disease course monitoring for myocardial infarction, treatment monitoring for medicines, and the like. The turbidimetric rapid detection kit provided by the invention realizes the integration of the reagents and the reaction device, and is simple, rapid and accurate to operate, high in sensitivity, strong in specificity, and low in detection cost; and the turbidimetric rapid detection kit is a detection / reagent measurement integrated kit suitable for outpatient and emergency treatment / clinical detection, and wide in instrument application range.

The invention relates to preparation of troponin I specific locus antibody and a detection kit thereof. A fixed point hydrolysis method is adopted to obtain the required three peptide sections of human cardiac troponin I (cTnI); the three peptide section are orderly subjected to immunogenicity modification and animal immunization so as to obtain high titer immune serum; the three peptide sections extracted in the hydrolysis are taken as the ligand and the polyacrylamide is taken as genin to prepare an affinity column; and finally the obtained high titer immune serum is subjected to immunoaffinity chromatography so as to obtain polyclonal antibodies having specific recognition on the three peptide sections. The antibodies have the advantages that: the affinity of the antibodies is higher than that of a corresponding monoclonal antibody, the specificity is equal to that of a corresponding monoclonal antibody, moreover, the preparation cost is lower, and the preparation process is simpler, compared to that of a monoclonal antibody. The obtained three polyclonal antibodies can be made into an immunity latex kit for a semi-automatic or automatic generation analysis instrument for detecting the content of cardiac troponin I (cTnI) in serum.

The invention relates to a troponin I latex-enhanced immunological turbidimetry detection kit which particularly comprises a first reagent and a second reagent. The first reagent comprises buffer solution, blocking agents, surface active agents, electrolyte, stabilizers, polyethylene glycol, chaotropic agents and preservatives; the second reagent comprises latex particles coated with human troponin I monoclonal antibodies, buffer solution, surface active agents, stabilizers and preservatives. The kit displays better interference resistance.

A cardiac troponin I ultra-sensitive detection reagent kit, a preparation method, and a detection method. The reagent kit comprises at least one first anti-cardiac troponin I antibody marked with a trace marker and at least one second anti-cardiac troponin I antibody coated on magnetic microspheres, the first anti-cardiac troponin I antibody and cardiac troponin I binding site being different from the second anti-cardiac troponin I antibody and cardiac troponin I binding site. The reagent kit may further comprise a diluent capable of significantly reducing non-specific binding in a detection process, so as to further increase the detection accuracy and sensitivity. The method using the reagent kit to detect cardiac troponin I sensitively and accurately detects the amount of cardiac troponin I in a sample, and provides more timely and reliable information for the early diagnosis and treatment of AMI.

The invention relates to the field of clinical immunology, particularly to colloidal gold test paper for quickly detecting troponin I and a preparation method for the colloidal gold test paper. The colloidal gold test paper comprises a coated film, a colloidal gold pad 1, a colloidal gold pad 2, a sample pad and a water absorbing pad which are mutually attached in a staggered way; the colloidal gold pad 1 is sprayed with a colloidal gold labeled cardiac troponin I resisting antibody; the colloidal gold pad 2 is sprayed with a colloidal gold labeled BSA (Bovine Serum Albumin)-resisting monoclonal antibody or PEG (Polyethylene Glycol)-resisting monoclonal antibody; and the coated film is coated with a cardiac troponin I resisting antibody detection line and a rabbit antimouse antibody quality control line. According to the improvement on the test paper strip, a PEG resisting technology and a BSA-resisting monoclonal antibody technology are introduced into the detection of the cardiac trooping I for the first time, so that the detection sensitivity of the cardiac trooping I is greatly improved and the clinical application of a traditional colloidal gold quick diagnosis test paper is enlarged.

The subject invention relates to antibodies to troponin I as well as methods of use thereof. In particular, such antibodies may be used to detect Troponin I in a patient and may also be used in the diagnosis of, for example, a myocardial infarction or acute coronary syndrome.

The present invention discloses one kind of human myocardial troponin I subunit nucleic acid adaptor and its application. The human myocardial troponin I subunit nucleic acid adaptor has the nucleotide sequence as shown and may be use in the early detection of myocardial troponin I, the clinical diagnosis of myocardial damage and the separation and purification of myocardial troponin I. The human myocardial troponin I subunit nucleic acid adaptor is oligonucleotide with relatively small molecular weight, may be synthesized chemically, and has affinity and specificity higher than that of antibody, easy labeling selectively in different parts, high repeatability and stability, easy preservation and excellent application foreground.

The invention provides a method for detecting cardiac troponin I (cTnI) and a cardiac troponin I detection kit prepared by applying the method. The method comprises the steps of coupling an antibody composition with polystyrene latex particles, then subjecting the coupled material to immunoreaction with corresponding antigen in a sample to be detected to form aggregated particles, and determining turbidity generated by the reactant under a wavelength of 400-700nm to obtain the content of cTnI in the sample to be detected. The antibody composition consists of at least three antibodies, and the antibodies are specific to cTnI without cross reaction with fast skeletal muscle troponin I and slow skeletal muscle troponin I. In addition, if one antibody in the antibody composition is sensitive to a certain factor influencing the accurate determination of cTnI in the sample to be detected, and then at least another antibody is not sensitive to the factor influencing the accurate determination of cTnI. The method of the invention is simple to operate, and has a wide scope of application, high precision, strong capability of resisting disturbance and low production cost.

The application provides a high-sensitivity magnetic particle chemiluminescence immunoassay kit for cardiac troponin I (hs-cTnI). The kit comprises a reagent R1, a reagent R2, a magnetic particle working solution and a cTnI antigen calibrator. The reagent R1 contains a biotin-labeled anti-cTnI polyclonal antibody; the reagent R2 contains two acridinium-ester-labeled anti-cTnI monoclonal antibodiesagainst different cTnI epitopes; the magnetic particle working solution includes streptavidin magnetic beads. In addition, the kit also can include a H2O2 solution and an alkaline solution. Besides,the application also provides a preparation method and purpose of the kit. The kit is capable of identifying multiple epitopes of the cTnI antigen, so that the analytical sensitivity and precision ofthe cardiac troponin I chemiluminescence immunoassay kit are improved.

The invention, which belongs to the technical fields of immunology and food safety analysis, relates to an enzyme-linked immunosorbent assay kit for detecting skeletal muscle troponin I of cattle or sheep, and a preparation method thereof and application thereof. The kit comprises an enzyme label plate coated with a capture antibody, a horseradish-peroxidase-labeled detection antibody, a skeletalmuscle troponin I standard solution of cattle or sheep, a substrate coloured solution, a stop solution, and a concentrated washing solution. The capture antibody is obtained by secretion of a hybridoma cell line 3A8 with the preservation number of CCTCC NO:C2018217; and the detection antibody is obtained by secretion of a hybridoma cell line 3D3 with the preservation number of CCTCC NO:C2018218. The kit has advantages of high sensitivity, high precision, high accuracy, and low cross-reaction rate and is suitable for large-scale sample detection.

A method for screening a substance that inhibits the onset of anti-cardiac troponin I autoantibody-related disease, a pharmaceutical composition and a base material for therapy of cardiac disease that contains the substance obtained by aforesaid method thereof, a therapeutic apparatus that removes anti-troponin I autoantibody for aforesaid antibody related disease, a method of making an animal model for evaluating cardiac disease characterized by administrating anti-cardiac troponin I antibody, a method of selection of a therapeutic substance for cardiac disease characterized by using aforesaid animals, and a diagnosis of dilated cardiomyopathy characterized by measuring anti-cardiac troponin I autoantibody. An apparatus of the present invention that removes anti-cardiac troponin I antibody and a pharmaceutical composition for therapy of the antibody related disease may be useful for a therapy and / or prevention of cardiac disease.

The invention relates to a preparation method and application of a multilevel micro-meter cubic zinc stannate composite material based photoelectrochemical sensor to cardiac troponin I and belongs tothe field of photoelectrochemical sensors. Polypyrrole / nitrogen subtracted graphite phase carbon nitride is taken as a template for synthesis of a multilevel structural Zn2SnO4 cube; nitrogen and sulfur doped graphene quantum dot N,S-GQDs is used for sensitizing Zn2SnO4 to improve its visible light absorption; the process of in-situ growth of CdS nanoparticles is carried out to obtain a multilevel micro-meter cubic zinc stannate composite material Zn2SnO4 / N,S-GQDs / Cds with photoelectric activity improved greatly; cardiac troponin I antibodies, bovine serum albumin and cardiac troponin I antigens are assembled to the composite material Zn2SnO4 / N,S-GQDs by means of the layer by layer self-assembly; thus, according to the excellent photoelectric activity of Zn2SnO4 / N,S-GQDs and binding of specificity of cardiac troponin I antigens and cardiac troponin I antibodies, ultra-sensitive detection to cardiac troponin I is achieved, which has a great significance in analysis and detection of cardiac troponin I.

Polypeptides corresponding to stable, circulating degradation products of troponin I (TnI) are described. The fragments comprise a sequence of the N-terminus of native cardiac TnI with 95-115 amino acid and additionally include fragments lacking about the 20-30 N-terminal amino acids. Utilities of these fragments and antibodies thereto include sensitive detection of myocardial infarction and purification of antibodies sensitive to the detection of troponin I degradation products.

The invention discloses an immune fluorescent test strip component for quickly quantitatively detecting troponin I, a detection card component comprising the immune fluorescent test strip component and preparation methods for the immune fluorescent test strip component and the detection card component. The test strip component comprises a test strip and an independently packaged platinum porphyrin labelled specific antibody, wherein the test strip comprises a substrate, an absorbent pad, an enveloping analysis film and a sample pad; the enveloping analysis film is provided with a detection line and a quality control line; a specific antibody enveloped by the detection line is a troponin I resistant monoclonal antibody; and a specific antibody enveloped by the quality control line is a rabbit IgG antibody. The detection card component comprises the test strip, a card box consisting of a cover plate and a back plate, and an independently packaged platinum porphyrin labelled specific antibody. An immune fluorescent rapid detection technology is adopted for detecting the level of the troponin I in a human body, the cost is low, the operation is simple, rapid and sensitive, the specificity is high, and the invention has significance for preventing cardiovascular and cerebrovascular diseases.

The invention relates to a detection kit for cardiac troponin I and a preparation method thereof. The detection kit for cardiac troponin I disclosed by the invention comprises an detection antibody and tracer-marked capture antibodies, wherein the capture antibodies are respectively two monoclonal antibodies combining different loci of the cardiac troponin I, and the detection antibody is a polyclonal antibody of the cardiac troponin I. The detection kit for cardiac troponin I and the preparation method thereof provided by the invention obviously improve the detection sensitivity and linear range, ensure accuracy and degree of precision at the same time, and can be used for efficient detection of cardiac troponin I by screening proper capture antibodies, detection antibody and combinationthereof detected by immunochromatography of the cardiac troponin I, and optimizing diluent components of the kit synchronously.

The invention discloses a troponin I resisting monoclonal antibody. An amino acid residue sequence of a light chain of the antibody is shown in SEQ ID No.1 and the amino acid residue sequence of a heavy chain is shown in SEQ ID No.2. The antibody is generated by a hybridoma cell line 5G9A10E3 with a preservation number of CCTCC NO.C201059 through secretion. The troponin I resisting monoclonal antibody can be applied to preparing various detection kits of the troponin I. A detection kit prepared by using the monoclonal antibody and a colloid gold immunochromatographic assay can ensure that the detection sensitivity reaches 97.74 percent, the detection specificity reaches 94.74 percent and the detection accuracy reaches 96.72 percent, and ensure that the Kappa detection value reaches 0.927 and far more than 0.75, has higher diagnosis consistency with the clinical diagnosis and can be used for auxiliary diagnosis for the acute myocardial imfarction disease.

The invention relates to a troponin I detection reagent and a preparation method thereof. The troponin I detection reagent is prepared from a first reagent and a second reagent in the volume ratio of 5: 1, wherein the first reagent contains 20-50mmol / L of buffer solution, 2%w / v of coagulant, 2%w / v of BSA and 0.1%-0.5%w / v of preservative; the second reagent contains 0.1%-0.5%w / v of colloid gold particles marked with anti-human cTnI antibodies, 2%-5%w / v of stabilizer, 20-50mmol / L of buffer solution and 0.1%-0.5%w / v of preservative; the diameter of the colloid gold particles marked with the anti-human cTnI antibodies ranges from 60nm to 90nm. The troponin I detection reagent has the advantages of being good in operability, high in analytical sensitivity, high in linearity, and quick, simple and convenient.

The invention belongs to the technical field of in-vitro detection, and particularly relates to a detection kit and a preparation method and application thereof. The detection kit comprises a magneticseparation reagent and an enzyme-labeled reagent; the magnetic separation reagent is prepared from troponin T antibody-coated gold magnetic particles or prepared from streptavidin-coated gold magnetic particles and a biotin-labeled troponin T antibody; and the enzyme-labeled reagent is prepared from an enzyme-labeled antibody polymer, wherein the enzyme-labeled antibody polymer is prepared from at least two enzyme-labeled antibodies and carbon bridges; the carbon bridges connect any two or more, adjacent or non-adjacent enzyme-labeled antibodies, and each carbon bridge has at least two connecting loci for connecting the enzyme-labeled antibodies; and each enzyme-labeled antibody is prepared from a detection antibody and a labeling enzyme coupled to the detection antibody, and the connecting loci are connected with the detection antibodies and / or the labeling enzymes. When chemiluminescence detection is conducted, a reaction signal value of a chemiluminescence method is amplified, thesensitivity of an antigen captured by the detection reagent can be enhanced, and the detection sensitivity is improved.

The invention relates to a reagent kit for detecting troponin I and a preparation and use method thereof. The reagent kit for detecting the troponin I comprises a base plate, a sample pad, a cross-linked substance pad, a connecting pad, a nitrocellulose membrane and absorbent paper which are connected sequentially are arranged on the base plate from one end to the other end, the cross-linked substance pad envelops cross-linked substances of fluorescent silica nanoparticle-anti-troponin I monoclonal antibodies, the nitrocellulose membrane is provided with a detection band and a quality control band, the detection band is arranged on one side close to the connecting pad and contains anti-troponin I monoclonal antibodies, and the quality control band is arranged on the other side away from the connecting pad and contains goat anti-mouse IgG (immunoglobulin G). The reagent kit for detecting the troponin I can achieve quantitative detection and high-sensitivity detection only by adopting a portable fluorescence detector, is simple in use, high in detection speed and low in cost and can be used for bedside diagnosis.

CTnI synthetic polypeptide antibody, its production and use are disclosed. The process is carried out by synthesizing polypeptide by 98-110 amino-acid at N end of amino-acid sequence RQLHARVDKVDEE, taking it as partial antigen to connect with protein carrier, preparing artificial immunogen, immunizing for animal, inducing organism effectively, generating immune response to obtain antibody, specific combined reacting cTnI synthetic polypeptide antibody with natural myoepicardial protein I molecule and coating cellulose nitrate film by the antibody, and reducing by tri-sodium citrate to obtain the final product. It's fast and has better sensitivity and specificity, and it can be used for acute myocardial infarction early diagnostic technology.