52 results about "Myoglobin" patented technology

The present invention relates to a fish or meat substitute or a pet food, comprising a myoglobin from or derived from eastern Pacific bonito, sword fish or Atlantic blue marlin.

The invention relates to methods, devices, and software for distinguishing hemoglobin (Hb) from myoglobin (Mb) in urine samples using peroxidase-reactive test strips. A time-resolved video of the color change on the test pad is recorded, and hue values are extracted and analyzed over time. Diagnostic differentiation between Hb and Mb is achieved by evaluating the hue-time curve, in particular via endpoint hue values and the ratio of integrated hue values in distinct time intervals. The invention further comprises a standardized optical setup for recording, a software-based evaluation algorithm, and a refillable cartridge for in-situ urine analysis devices containing appropriate test fields. The invention enables combined testing with additional urine parameters and is suitable for medical and non-clinical applications such as sport and home diagnostics.

The application belongs to the technical field of biological detection, and discloses a cardiovascular marker control article and a preparation method thereof. The cardiovascular marker control article comprises a serum matrix, antigens, a preservative and a stabilizer. The antigens include cardiac troponin I antigens, creatine kinase isoenzyme antigens, myoglobin antigens, amino-terminal brain natriuretic peptide precursor antigens, brain natriuretic peptide antigens, D-dimer antigens, heart-type fatty acid-binding protein antigens, myeloperoxidase antigens and lipoprotein phospholipase A2 antigens. The stabilizer includes at least one of D-trehalose, sucrose, glycine, inositol and polyethylene glycol. The cardiovascular marker control article can cover various cardiovascular disease markers, including heart failure, pulmonary embolism, vasculitis and atherosclerosis, myocardial injury, myocardial infarction and the like. The control article can be used as a third-party control article for indoor and inter-laboratory quality control of a cardiovascular disease screening and auxiliary diagnosis measurement system.

The application discloses a method for detecting the concentration of dissolved CO in fermentation liquor. The method comprises the following steps: (1) preparing CO saturated aqueous solution, oxygen-free aqueous solution, sodium hydrosulfite solution as a reference solution and deoxygenated myoglobin solution; (2) preparing an absorbance-CO saturation standard curve; (3) sampling and detection: taking a sample from the fermentation liquor as a sample to be detected by a sampling device, injecting the sample to be detected into a cuvette, adding 2.5 mL of deoxygenated myoglobin solution in the cuvette in advance and sealing the cuvette, adjusting zero with the reference solution, detecting the absorbance of the cuvette containing the sample to be detected at a wavelength of 415-425 nm by a spectrophotometer, substituting the obtained absorbance into the absorbance-CO saturation standard curve obtained in step (2) to obtain the corresponding CO saturation, and then converting the CO saturation into the concentration of dissolved CO according to the known CO solubility. The detection method has the characteristics of taking and measuring immediately, rapidness and simplicity.

The invention discloses a medium-temperature beef fresh-keeping production method, and belongs to the technical field of medium-temperature beef production. The method comprises the following steps: segmenting and classifying slaughtering parts; hanging the cut meat for air cooling, and performing gradient cooling at the same time to complete first treatment; controlling the oxygen concentration, and performing cold plasma treatment to complete second treatment; and then carrying out quick-cooling drying, packaging and cold chain management in sequence. The juice loss is inhibited through air cooling control in combination with gradient cooling; hypoxia and cold plasmas cooperate to block myohemoglobin oxidation and maintain the value of redness a; all the steps have a synergistic effect to solve the four industrial pains of water return, decoloration, poor tenderness and short shelf life of the medium-temperature beef.

Embodiments of the present disclosure generally relate to protein powder compositions and methods for preparing the same. In one or more embodiments, methods include coating a plurality of protein powder particles containing myoglobin with an aluminum oxide coating to form a plurality of coated particles during an atomic layer coating (ALC) process. The protein powder compositions have improved properties over the corresponding uncoated protein powder. The protein powder compositions have greater flowability, density, and a greater level of recoverability over the corresponding uncoated protein powder.

The application discloses a composition for improving the antioxidant activity of vitamin C and derivatives thereof. By testing vitamin C solutions and vitamin C derivative solutions added with different concentrations of mussel myoglobin without a dopa group, it is found that when the mass ratio of vitamin C or vitamin C derivatives and mussel myoglobin without a dopa group is 1:0.001-1, the stability of vitamin C or vitamin C derivatives can be improved, and a new approach for effectively enhancing the antioxidant activity of vitamin C and derivatives thereof in cosmetics is provided.

The invention discloses a plasma protein marker for identifying or assisting in identifying parathyroid cancer and parathyroid adenoma and application of the plasma protein marker. The method comprises the following steps: firstly, collecting plasma specimens of a parathyroid carcinoma patient and a parathyroid adenoma patient before an operation, and screening through an unmarked quantitative proteomics technology to obtain 24 differential expression proteins; on the basis of the other part of plasma specimens of the parathyroid carcinoma patient and the parathyroid adenoma patient collected before the operation, the 24 differential expression proteins are verified through a parallel reaction monitoring technology; finally, three plasma protein markers (myoglobin, coronin-1A and a polyimmunoglobulin receptor) which can be used for identifying the parathyroid carcinoma and the parathyroid adenoma are obtained. Experiments prove that the plasma protein marker or the combination thereof can be used for auxiliary differential diagnosis of parathyroid carcinoma and parathyroid adenoma, and has important significance for improving the accuracy of preoperative diagnosis of parathyroid carcinoma.

The application provides a clinical rehabilitation data management and evaluation system for rhabdomyolysis patients, and relates to a medical information management system.The system comprises an index acquisition module, a rate detection module, a curvature analysis module, a node identification module and a trend construction module.In the application, the rate difference sequence of creatine kinase and myoglobin and the curvature sequence of urine sodium and blood creatinine are combined to break through the limitations of traditional single-parameter static analysis, realize dynamic capture of multi-index collaborative trend, construct a joint evolution label system by combining the consistency discrimination of creatine kinase and blood potassium concentration change direction, accurately locate the multi-system interaction risk nodes, and statistically distribute the direction consistency proportion of myoglobin and blood creatinine in the correlation period, so as to convert the discrete parameter correlation into a dynamic visual model.The method solves the problems of data dispersion misjudgment and collaborative evolution mode fragmentation in the traditional technology, and provides a multi-dimensional dynamic evaluation framework for the rhabdomyolysis rehabilitation process in clinical practice.

The application discloses a kind of broad-spectrum general-purpose nano-coating probiotics based on Mytilus edulis mucin and a preparation method and application thereof.The method accelerates the deposition of Mytilus edulis mucin on the surface of probiotics by means of non-covalent bond, hydrogen bond, cation-pi and other supramolecular interaction modes between Mytilus edulis mucin and bacteria, and realizes the independent coating of probiotics by complexing and coordinating the catechol group in Mytilus edulis mucin with cations and driving it to self-assemble into nanoscale aggregates.The Mytilus edulis mucin nano-coating provided by the application is not only suitable for encapsulating aerobic bacteria, but also has a protective effect on anaerobic bacteria, and can realize the synchronous release of probiotics.More importantly, the addition of cations enhances the adhesion ability of probiotics while improving the density of the coating network structure, resulting in nano-coating probiotics with high stability and biocompatibility.The nano-coating probiotics provided by the application have simple and efficient preparation process, are suitable for large-scale production, and are expected to become an ideal carrier for probiotic delivery.

1. The name of the design product: packaging kit (medical mussel mucin care solution). 2. The use of the design product: for product packaging. 3. The design points of the design product: in the pattern. 4. The picture or photo that best indicates the design points: main view of kit 2. 5. Other circumstances that need to be explained: The design product is a kit product, so it is used and sold as a set. Kit 1 is an inner packaging bottle, kit 2 is an outer packaging box, and kit 1 is packed into kit 2 for sale and use.

We propose a new method to support the physical condition management of athletes. [Solution] The system for supporting the physical condition management of athletes comprises a measuring device for measuring myoglobin contained in the athlete's blood, and a server that records the athlete's exercise load and the athlete's myoglobin measurement value. The server provides the athlete's exercise load and the athlete's measurement value in response to a request from a terminal.

Embodiments of this disclosure generally relate to protein powder compositions and methods for preparing them. In one or more embodiments, the method includes coating a plurality of protein powder particles containing myoglobin with an aluminum oxide coating to form a plurality of coated particles during an atomic layer coating (ALC) process. The protein powder composition has improved properties compared to the corresponding uncoated protein powder. The protein powder composition has greater flowability, density, and a higher level of recoverability compared to the corresponding uncoated protein powder.

The application discloses a kind of myoglobin preparation process for chemiluminescence immunoassay quality control product, it is related to myoglobin preparation technical field, comprising the following steps: raw material pretreatment: after removing impurities of fresh pig heart muscle tissue, it is placed in automatic cleaning cutting equipment and is cut and cleaned, myoglobin extraction: muscle tissue after pretreatment is placed in grinding mechanism, and extraction fluid is added to grind, centrifugal separation: after extraction, mixed liquid is placed in high-speed centrifugal separation equipment and is centrifuged, to obtain supernatant, purification treatment: supernatant is purified by multistage filtration separation equipment, stabilization treatment: after purification, myoglobin solution is added with stabilizer, placed in gradient temperature control stirring system and stirred and mixed, low-temperature preservation, solve the problem that muscle tissue is cut into meat foam by manual shearing mode, grinding is not enough, so that separation effect is poor, leading to the problem that myoglobin purity is not high and activity is easily affected.

The present invention provides a heterophilic antibody blocker comprising a light chain and a heavy chain; the amino acid sequence of the light chain is shown in SEQ ID NO.1, and the amino acid sequence of the heavy chain is shown in SEQ ID NO.2. The present invention also provides a use of the above-mentioned heterophilic antibody blocker in the preparation of a myoglobin detection kit and a corresponding kit. The heterophilic antibody blocker of the present invention is a self-developed product designed specifically for myoglobin detection and can effectively remove interfering substances in serum; the use of this heterophilic antibody blocker can greatly improve the performance of the reagent, reduce the false positive rate, and provide a reliable guarantee for the test results.

The present application relates to a kind of meat product colorant based on double enzyme cascade reaction and coloration method and application, belong to meat science and technology field.The meat product colorant is composed of recombinant glucose dehydrogenase and one oxygen fusion enzyme NOS-YkuN-YumC of producing nitric oxide.The present application is by constructing the nicotinamide adenine dinucleotide phosphate (NADPH) regeneration system based on glucose dehydrogenase in recombinant bacillus subtilis, realize the continuous supply of key cofactor NADPH, to significantly enhance the catalytic activity of one oxygen fusion enzyme NOS-YkuN-YumC of producing nitric oxide under low temperature condition.It is applied to meat product, and the produced one oxygen is combined with myoglobin efficiently, generates nitrosylmyoglobin, so that meat product can obtain ideal red color even in low temperature processing with sodium nitrite treatment.The present application provides efficient and feasible solution for replacing nitrite coloration and improving meat product safety.

The invention discloses application of an iron-containing substance. The invention finds that the iron-containing substance can be used for preparing the medicine for treating leucopenia and / or thrombocytopenia; the iron-containing substance is selected from myoglobin and a derivative thereof, hemoglobin and a derivative thereof, ferrous sulfate, ferrous fumarate, ferrous gluconate, a polyferose compound, ferrous succinate, iron amino acid chelate, heme, heme iron polypeptide, ferric hydroxide polymaltose, ferrous lactate, ferric pyrophosphate and iron dextran; and one or more of iron hydroxymaltose. The iron-containing substance can effectively increase the number of leukocytes and / or platelets.

To provide a gene construct comprising a nucleic acid encoding a protein, in a meat substitute comprising the myoglobin protein, a host cell comprising the gene construct, and a method for producing myoglobin or a meat substitute.SOLUTION: A method for producing a meat substitute comprising bovine myoglobin, the method comprising: a): a step of providing a host cell comprising a gene construct, wherein the gene construct is represented by a specific sequence; b) a step of culturing the host cell in a medium suitable for producing bovine myoglobin represented by a specific sequence different from the foregoing; c) a step of recovering the myoglobin; d) a step of purifying the myoglobin; and e) a step of blending the purified myoglobin into the meat substitute.SELECTED DRAWING: None

To provide RcoM protein-based carbon monoxide scavengers and preparations for treating carbon monoxide poisoning.SOLUTION: Disclosed herein is a method for rapid elimination of carbon monoxide (CO) from CO-bound hemoglobin, myoglobin and cytochrome C oxidase in a subject with CO poisoning. The disclosed therapeutic method comprises use of a rationally designed and modified regulatory factor of CO metabolism (RcoM) protein and pharmaceutical compositions thereof, which scavenge carbon monoxide from poisoned tissue. The recombinant RcoM composition is infused into blood to rapidly sequester carbon monoxide and limit the toxic effects of carbon monoxide on cellular respiration, oxygen transport and oxygen utilization.SELECTED DRAWING: None

The present invention discloses a method for precision engineering of protein bioconjugates by harnessing complex multi-equilibria systems. The method allows for exclusive control over reaction pathways, achieving highly selective outcomes, even with complex substrates such as proteins. By manipulating proteinogenic functionalities, the invention enables pathway selectivity, chemoselectivity, and site selectivity, leading to single-site modifications in proteins. The versatility of the present invention is demonstrated through diverse protein examples, including insulin, RNase A, ubiquitin, cytochrome C, myoglobin, α-lactalbumin, and serum albumins, showcasing its broad applicability in protein engineering. Additionally, the method facilitates the synthesis of antibody-fluorophore conjugates (AFC) and antibody-drug conjugates (ADC), providing promising prospects for therapeutic applications.

This invention discloses a strain of *Lactobacillus brevis* and its applications, belonging to the field of food microbiology technology. The invention provides a strain of *Lactobacillus brevis* CHOL1, isolated from sauerkraut. After safety evaluation, this strain is suitable for use in food. It exhibits a strong ability to degrade nitrite and can also react with myoglobin to generate nitrosomyoglobin. Inoculation of this strain into fermented meat products revealed that it can reduce nitrite and nitrosamine residues while improving the color of the meat products. As a starter culture, this strain can improve the quality and safety of fermented meat products and can serve as a natural and effective substitute for nitrite in fermented meat products.

The application discloses a kind of bionic recombination III type Mytilus edulis mucin and its preparation method and application.The recombination III type Mytilus edulis mucin is fused by Mfp-3 of natural Mytilus edulis and the protein that constitutes Mytilus edulis silk fiber skeleton.In the preparation process, fusion gene is co-expressed with tyrosinase gene, and the tyrosine residue in recombination Mytilus edulis mucin is modified with dopa using expressed tyrosinase, to obtain the recombination III type Mytilus edulis mucin rich in dopa group, which can be dissolved and stable at neutral pH, and has high yield, high purity, low endotoxin content, fully meet the application needs in the field of drug, medical device and cosmetic.

The invention relates to methods, devices, and software for distinguishing hemoglobin (Hb) from myoglobin (Mb) in urine samples using peroxidase-reactive test strips. A time-resolved video of the color change on the test pad is recorded, and hue values are extracted and analyzed over time. Diagnostic differentiation between Hb and Mb is achieved by evaluating the hue-time curve, in particular via endpoint hue values and the ratio of integrated hue values in distinct time intervals. The invention further comprises a standardized optical setup for recording, a software-based evaluation algorithm, and a refillable cartridge for in-situ urine analysis devices containing appropriate test fields. The invention enables combined testing with additional urine parameters and is suitable for medical and non-clinical applications such as sport and home diagnostics.

The transgenic plants engineered to express animal-derived proteins, such as myoglobin, casein, chymosin, hemoglobin and actin and other animal-derived proteins, and the nucleic acids encoding the same are described herein. Methods of making and identifying the transgenic plants, processing the seeds from the transgenic plants that contain the expressed animal-derived proteins, isolating and purifying the expressed proteins, and utilizing the expressed proteins in food are provided. Food and food ingredients that include myoglobin, casein, chymosin, hemoglobin and actin are also disclosed herein. Genes encoding myoglobin, casein, hemoglobin and actin forms that have been modified to improve performance as components of food are described.

The invention belongs to the technical field of biology, and relates to a colorimetric probe for detecting myoglobin and a detection method. The colorimetric probe comprises a reagent A and a reagent B: a solution containing iodide ions; the preparation method of the reagent A comprises the following steps: adding a reducing agent into a solution containing a copper salt and a coordination stabilizer, stirring, adding a water-soluble gold salt or gold acid when the solution turns from red to green, and continuously stirring for 1-100 minutes to obtain a copper-gold nanoparticle solution; the thioacetamide is subjected to a hydrolysis reaction in an acid solution to generate hydrogen sulfide, then the copper-gold nanoparticle solution obtained in the step S1 is added for a reaction, and a hydrogen sulfide modified copper-gold nanoparticle solution is obtained. Through a coupling mechanism of acidic H2S in-situ release-Au-S bond binding-I-etching, nanomole-level, high-selectivity and label-free colorimetric detection of myoglobin is realized, and the method has sensitivity, reliability, economical efficiency and field applicability, and can be widely applied to rapid screening.

Described herein a method for flavouring a meat substitute. The invention in particular relates to a method wherein the steps comprises adding an myoglobin composition and a fat composition.

The invention provides an application of a mitochondrial targeting antioxidant in improving muscle lipid oxidation, and belongs to the technical field of meat preservation. According to the invention, the muscle is only soaked in the mitoquinone solution, so that the oxidation of lipid in the muscle can be obviously improved. Meanwhile, the release of mitochondria ROS (reactive oxygen species) can be remarkably inhibited by the treatment of the mitoquinone, the total antioxidant capacity T-AOC of the muscle is improved, the TBARS value of the slaughtered muscle can be remarkably reduced, and the lipid oxidation is remarkably improved; main lipid molecules PE, DG and PC of the mitochondrial membrane can be protected, further mitochondria is protected, the mitochondrial double-layer membrane can still keep a complete structure and a regular shape within a specific storage time, the biological characteristics of the mitochondrial double-layer membrane are guaranteed, bad flavor substances are reduced, myoglobin discoloration is delayed, natural distribution of lipid is reserved, and the mitochondrial membrane has a good application prospect. The method maintains the bright color, delicate tenderness and mellow flavor of meat products, breaks through the limitation of the traditional technology, and provides a new method for meat preservation.

1. Name of the product in this design: Packaging kit (Medical mussel adhesive protein care solution). 2. Purpose of this design: for product packaging. 3. The key design feature of this product is its pattern. 4. The image or photograph that best illustrates the design highlights: the main view of Suite 2. 5. Other situations requiring explanation: This design product is a kit product, so it is used and sold as a set. Kit 1 is a packaging bottle, and kit 2 is a packaging box. Kit 1 is placed inside kit 2 for sale and use.

The invention relates to methods, devices, and software for distinguishing hemoglobin (Hb) from myoglobin (Mb) in urine samples using peroxidase-reactive test strips. A time-resolved video of the color change on the test pad is recorded, and hue values are extracted and analyzed over time. Diagnostic differentiation between Hb and Mb is achieved by evaluating the hue-time curve, in particular via endpoint hue values and the ratio of integrated hue values in distinct time intervals. The invention further comprises a standardized optical setup for recording, a software-based evaluation algorithm, and a refillable cartridge for in-situ urine analysis devices containing appropriate test fields. The invention enables combined testing with additional urine parameters and is suitable for medical and non-clinical applications such as sport and home diagnostics.