34 results about "Myoglobin" patented technology

The application belongs to the technical field of biological detection, and discloses a cardiovascular marker control article and a preparation method thereof. The cardiovascular marker control article comprises a serum matrix, antigens, a preservative and a stabilizer. The antigens include cardiac troponin I antigens, creatine kinase isoenzyme antigens, myoglobin antigens, amino-terminal brain natriuretic peptide precursor antigens, brain natriuretic peptide antigens, D-dimer antigens, heart-type fatty acid-binding protein antigens, myeloperoxidase antigens and lipoprotein phospholipase A2 antigens. The stabilizer includes at least one of D-trehalose, sucrose, glycine, inositol and polyethylene glycol. The cardiovascular marker control article can cover various cardiovascular disease markers, including heart failure, pulmonary embolism, vasculitis and atherosclerosis, myocardial injury, myocardial infarction and the like. The control article can be used as a third-party control article for indoor and inter-laboratory quality control of a cardiovascular disease screening and auxiliary diagnosis measurement system.

The application discloses a method for detecting the concentration of dissolved CO in fermentation liquor. The method comprises the following steps: (1) preparing CO saturated aqueous solution, oxygen-free aqueous solution, sodium hydrosulfite solution as a reference solution and deoxygenated myoglobin solution; (2) preparing an absorbance-CO saturation standard curve; (3) sampling and detection: taking a sample from the fermentation liquor as a sample to be detected by a sampling device, injecting the sample to be detected into a cuvette, adding 2.5 mL of deoxygenated myoglobin solution in the cuvette in advance and sealing the cuvette, adjusting zero with the reference solution, detecting the absorbance of the cuvette containing the sample to be detected at a wavelength of 415-425 nm by a spectrophotometer, substituting the obtained absorbance into the absorbance-CO saturation standard curve obtained in step (2) to obtain the corresponding CO saturation, and then converting the CO saturation into the concentration of dissolved CO according to the known CO solubility. The detection method has the characteristics of taking and measuring immediately, rapidness and simplicity.

Embodiments of the present disclosure generally relate to protein powder compositions and methods for preparing the same. In one or more embodiments, methods include coating a plurality of protein powder particles containing myoglobin with an aluminum oxide coating to form a plurality of coated particles during an atomic layer coating (ALC) process. The protein powder compositions have improved properties over the corresponding uncoated protein powder. The protein powder compositions have greater flowability, density, and a greater level of recoverability over the corresponding uncoated protein powder.

The invention discloses a plasma protein marker for identifying or assisting in identifying parathyroid cancer and parathyroid adenoma and application of the plasma protein marker. The method comprises the following steps: firstly, collecting plasma specimens of a parathyroid carcinoma patient and a parathyroid adenoma patient before an operation, and screening through an unmarked quantitative proteomics technology to obtain 24 differential expression proteins; on the basis of the other part of plasma specimens of the parathyroid carcinoma patient and the parathyroid adenoma patient collected before the operation, the 24 differential expression proteins are verified through a parallel reaction monitoring technology; finally, three plasma protein markers (myoglobin, coronin-1A and a polyimmunoglobulin receptor) which can be used for identifying the parathyroid carcinoma and the parathyroid adenoma are obtained. Experiments prove that the plasma protein marker or the combination thereof can be used for auxiliary differential diagnosis of parathyroid carcinoma and parathyroid adenoma, and has important significance for improving the accuracy of preoperative diagnosis of parathyroid carcinoma.

1. The name of the design product: packaging kit (medical mussel mucin care solution). 2. The use of the design product: for product packaging. 3. The design points of the design product: in the pattern. 4. The picture or photo that best indicates the design points: main view of kit 2. 5. Other circumstances that need to be explained: The design product is a kit product, so it is used and sold as a set. Kit 1 is an inner packaging bottle, kit 2 is an outer packaging box, and kit 1 is packed into kit 2 for sale and use.

We propose a new method to support the physical condition management of athletes. [Solution] The system for supporting the physical condition management of athletes comprises a measuring device for measuring myoglobin contained in the athlete's blood, and a server that records the athlete's exercise load and the athlete's myoglobin measurement value. The server provides the athlete's exercise load and the athlete's measurement value in response to a request from a terminal.

Embodiments of this disclosure generally relate to protein powder compositions and methods for preparing them. In one or more embodiments, the method includes coating a plurality of protein powder particles containing myoglobin with an aluminum oxide coating to form a plurality of coated particles during an atomic layer coating (ALC) process. The protein powder composition has improved properties compared to the corresponding uncoated protein powder. The protein powder composition has greater flowability, density, and a higher level of recoverability compared to the corresponding uncoated protein powder.

The application discloses a kind of myoglobin preparation process for chemiluminescence immunoassay quality control product, it is related to myoglobin preparation technical field, comprising the following steps: raw material pretreatment: after removing impurities of fresh pig heart muscle tissue, it is placed in automatic cleaning cutting equipment and is cut and cleaned, myoglobin extraction: muscle tissue after pretreatment is placed in grinding mechanism, and extraction fluid is added to grind, centrifugal separation: after extraction, mixed liquid is placed in high-speed centrifugal separation equipment and is centrifuged, to obtain supernatant, purification treatment: supernatant is purified by multistage filtration separation equipment, stabilization treatment: after purification, myoglobin solution is added with stabilizer, placed in gradient temperature control stirring system and stirred and mixed, low-temperature preservation, solve the problem that muscle tissue is cut into meat foam by manual shearing mode, grinding is not enough, so that separation effect is poor, leading to the problem that myoglobin purity is not high and activity is easily affected.

The present application relates to a kind of meat product colorant based on double enzyme cascade reaction and coloration method and application, belong to meat science and technology field.The meat product colorant is composed of recombinant glucose dehydrogenase and one oxygen fusion enzyme NOS-YkuN-YumC of producing nitric oxide.The present application is by constructing the nicotinamide adenine dinucleotide phosphate (NADPH) regeneration system based on glucose dehydrogenase in recombinant bacillus subtilis, realize the continuous supply of key cofactor NADPH, to significantly enhance the catalytic activity of one oxygen fusion enzyme NOS-YkuN-YumC of producing nitric oxide under low temperature condition.It is applied to meat product, and the produced one oxygen is combined with myoglobin efficiently, generates nitrosylmyoglobin, so that meat product can obtain ideal red color even in low temperature processing with sodium nitrite treatment.The present application provides efficient and feasible solution for replacing nitrite coloration and improving meat product safety.

This invention discloses a strain of *Lactobacillus brevis* and its applications, belonging to the field of food microbiology technology. The invention provides a strain of *Lactobacillus brevis* CHOL1, isolated from sauerkraut. After safety evaluation, this strain is suitable for use in food. It exhibits a strong ability to degrade nitrite and can also react with myoglobin to generate nitrosomyoglobin. Inoculation of this strain into fermented meat products revealed that it can reduce nitrite and nitrosamine residues while improving the color of the meat products. As a starter culture, this strain can improve the quality and safety of fermented meat products and can serve as a natural and effective substitute for nitrite in fermented meat products.

The application discloses a kind of bionic recombination III type Mytilus edulis mucin and its preparation method and application.The recombination III type Mytilus edulis mucin is fused by Mfp-3 of natural Mytilus edulis and the protein that constitutes Mytilus edulis silk fiber skeleton.In the preparation process, fusion gene is co-expressed with tyrosinase gene, and the tyrosine residue in recombination Mytilus edulis mucin is modified with dopa using expressed tyrosinase, to obtain the recombination III type Mytilus edulis mucin rich in dopa group, which can be dissolved and stable at neutral pH, and has high yield, high purity, low endotoxin content, fully meet the application needs in the field of drug, medical device and cosmetic.

The invention relates to methods, devices, and software for distinguishing hemoglobin (Hb) from myoglobin (Mb) in urine samples using peroxidase-reactive test strips. A time-resolved video of the color change on the test pad is recorded, and hue values are extracted and analyzed over time. Diagnostic differentiation between Hb and Mb is achieved by evaluating the hue-time curve, in particular via endpoint hue values and the ratio of integrated hue values in distinct time intervals. The invention further comprises a standardized optical setup for recording, a software-based evaluation algorithm, and a refillable cartridge for in-situ urine analysis devices containing appropriate test fields. The invention enables combined testing with additional urine parameters and is suitable for medical and non-clinical applications such as sport and home diagnostics.

The invention belongs to the technical field of biology, and relates to a colorimetric probe for detecting myoglobin and a detection method. The colorimetric probe comprises a reagent A and a reagent B: a solution containing iodide ions; the preparation method of the reagent A comprises the following steps: adding a reducing agent into a solution containing a copper salt and a coordination stabilizer, stirring, adding a water-soluble gold salt or gold acid when the solution turns from red to green, and continuously stirring for 1-100 minutes to obtain a copper-gold nanoparticle solution; the thioacetamide is subjected to a hydrolysis reaction in an acid solution to generate hydrogen sulfide, then the copper-gold nanoparticle solution obtained in the step S1 is added for a reaction, and a hydrogen sulfide modified copper-gold nanoparticle solution is obtained. Through a coupling mechanism of acidic H2S in-situ release-Au-S bond binding-I-etching, nanomole-level, high-selectivity and label-free colorimetric detection of myoglobin is realized, and the method has sensitivity, reliability, economical efficiency and field applicability, and can be widely applied to rapid screening.

Described herein a method for flavouring a meat substitute. The invention in particular relates to a method wherein the steps comprises adding an myoglobin composition and a fat composition.

1. Name of the product in this design: Packaging kit (Medical mussel adhesive protein care solution). 2. Purpose of this design: for product packaging. 3. The key design feature of this product is its pattern. 4. The image or photograph that best illustrates the design highlights: the main view of Suite 2. 5. Other situations requiring explanation: This design product is a kit product, so it is used and sold as a set. Kit 1 is a packaging bottle, and kit 2 is a packaging box. Kit 1 is placed inside kit 2 for sale and use.

1. The name of the design product: packaging box (recombinant mussel myosin repair dressing). 2. The use of the design product: for the packaging of medicines. 3. The design points of the design product: the combination of patterns and colors. 4. The picture or photo that best shows the design points: perspective view. 5. The design for which protection is sought contains colors.

The invention provides a mirror image aptamer 5 '-Biotin L-PEA1-20 combined with myoglobin, the mirror image aptamer 5'-Biotin L-PEA1-20 combined with myoglobin shows excellent combining capacity in the aspect of myoglobin combination, the unique mirror image structure of the mirror image aptamer also endows the mirror image aptamer 5 '-Biotin L-PEA1-20 with extremely high enzyme digestion and degradation resistance, and the mirror image aptamer can keep high stability in the presence of DNase I and EXO I. The method has important significance in related research of myoglobin, can be widely applied to multiple fields of medical diagnosis, drug development, biotechnology and the like, and particularly has remarkable advantages in accurate detection and targeted research of myoglobin in a complex physiological environment.

Protein separation can be provided based on a High Performance Countercurrent Membrane Purification (HPCMP), which can exploit highly selective diffusive transport across thin walls of a hollow fiber membrane for separation of proteins. Embodiments of an HPCMP system can separate mixtures of multiple proteins (e.g. separate BSA and Mb) or other biological material and provide high yields (e.g. achieving greater than 98% yield of both proteins with purification factors greater than 100-fold, etc.). Embodiments of a HPCMP system can be configured for high resolution separations in the preparation of biopharmaceuticals and natural protein products. Other embodiments can be utilized in other protein separation environments or biological material separation environments.

This document discloses binding moiety (e.g., antibody)-oligonucleotide (e.g., PMO) conjugates and pharmaceutical compositions that induce alterations in misspliced ​​myoglobin mRNA transcripts to induce DMD exon 45 skipping. The description also includes methods for treating muscular dystrophy, including Duchenne muscular dystrophy, comprising administering such conjugates or pharmaceutical compositions that induce alterations in misspliced ​​myoglobin mRNA transcripts to induce DMD exon 45 skipping.

The application discloses a method for culturing human periodontal ligament stem cells by using self-assembled mussel myoglobin. The method comprises the following steps: dissolving the self-assembled mussel myoglobin in PBS to adjust the pH to 7.0-8.0, and filtering and sterilizing; adding the self-assembled mussel myoglobin into a cell culture medium to prepare a working culture medium with a final concentration of 10-100 mu g / mL; resuspending the cells after digesting hPDLSCs, and adjusting the density to 3.0*10<5>-3.5*10<5> per mL; using the hanging drop culture method, 20-30 mu L of each drop is inoculated in the inner side of a culture dish cover, and human periodontal ligament stem cells are obtained after 2-5 days of culture. The wet adhesion characteristics of the self-assembled mussel myoglobin are used to significantly improve the structural stability of stem cell spheres and the internal cell survival rate, and to enhance the stemness maintenance, paracrine function, immunoregulatory capacity and multi-directional differentiation potential of stem cells. The cell spheres prepared by the method can be used for tissue repair, and especially show excellent repair effect in the treatment of thin endometrium.

The invention relates to the technical field of cosmetic preparations, in particular to a skin repair polypeptide composition which is composed of a signal peptide group A and a synergistic carrier group B, the group A signal peptide group comprises palmitoyl tripeptide-1, palmitoyl pentapeptide-4, palmitoyl heptapeptide-3, acetyl hexapeptide-8, acetyl octapeptide-3, acetyl decapeptide-5, tripeptide-1, hexapeptide-11 and nonapeptide-1; the group A signal peptide group comprises a group B and a group C; the group B synergistic carrier is prepared from medical-grade mussel mucin, schizophyllum commune polysaccharide, an oily component and water. According to the invention, the medical-grade mussel mucin is used as a polypeptide synergistic carrier, so that the transdermal absorption rate of the polypeptide is remarkably improved, the problem of low transdermal absorption rate of the traditional polypeptide is solved, and the active ingredients can better permeate into the deep layer of the skin to play a role.

The application discloses a one-oxygen-based fusion enzyme color developing agent and application. The color developing agent comprises a one-oxygen-based fusion enzyme, and the fusion enzyme is combined by a nitric oxide synthase, a flavoprotein and a flavoprotein reductase in sequence and two by two through a connecting peptide. The fusion enzyme provided by the application has high enzyme activity, can catalyze a large amount of one-oxygen, and can effectively combine myoglobin in meat products to generate nitroso-myoglobin, effectively improve the red color of the meat products, and obtain a color developing effect equivalent to that of sodium nitrite, thereby providing a very practically applicable solution for replacing the sodium nitrite color developing of the meat products and improving the safety of the meat products.

The present application relates to a kind of soluble expression of mussel mucin polypeptide and its preparation method and application, the soluble expression of mussel mucin polypeptide is at least one of MAP-PEP1, MAP-PEP2, the amino acid sequence of the MAP-PEP1 as shown in SEQ ID NO.1, the amino acid sequence of the MAP-PEP2 as shown in SEQ ID NO.2.The mussel mucin polypeptide MAP-PEP1, MAP-PEP2 rich in positive charge and DOPA group obtained by the present application can effectively destroy the cell membrane of gram-positive bacteria and gram-negative bacteria, induce oxidative stress response, inhibit bacterial proliferation;Also can promote cell adhesion, migration and proliferation, help wound healing, also have certain efficacy in free radical scavenging, have the great potential to become the next generation of multifunctional bioactive material, provide theoretical basis for being widely used in wound care, implant material, drug delivery, cosmetics and other fields.

The present disclosure provides methods of producing heme proteins in transgenic plants, plant tissues, or plant cells, as well as describing the expression of these heme proteins in seeds. Also, the present disclosure provides transgenic plants expressing the heme proteins, myoglobin and hemoglobin, by introducing and integrating the recombinant DNA constructs into the host genetic material of the subject plants. The specific combination of regulatory elements disclosed herein allows for high heme protein expression level in seeds.

Disclosed is a system for supporting health management of an athletic player, comprising: a measuring device configured to measure myoglobin contained in blood of the player; and a server configured to record an exercise load of the player and a measured value of the myoglobin of the player, wherein the server is configured to provide the exercise load of the player and the measured value of the player in response to a request from a terminal.

Provided is a method for measuring heme pigments in meat more easily than in the past without using special equipment.　Heme pigments are extracted from meat to obtain an extract, and the post-oxidation extract is dispensed to obtain a first extract and a second extract. The pH of the first extract is adjusted to 5.0-7.5 and the pH of the second extract is adjusted to 8.0-10.0, the extracts are oxidized, and measurements are taken of a first absorbance, which is the absorbance of the first extract at a predetermined wavelength, and a second absorbance, which is the absorbance of the second extract. The myoglobin concentration and the hemoglobin concentration in the meat are calculated from the difference between the measurements taken for the first absorbance and second absorbance, whereby the heme pigments in the meat is measured.

The invention relates to a meat product color former based on double-enzyme cascade reaction, a color development method and application, and belongs to the technical field of meat science. The color former for meat products is composed of recombinant glucose dehydrogenase and fusion enzyme NOS-YkuN-YumC capable of producing nitric oxide. According to the invention, a glucose dehydrogenase-based nicotinamide adenine dinucleotide phosphate (NADPH) regeneration system is constructed in recombinant bacillus subtilis, and continuous supply of a key cofactor NADPH is realized, so that the catalytic activity of a fusion enzyme NOS-YkuN-YumC for producing nitric oxide under a low-temperature condition is remarkably enhanced. When the method is applied to meat products, generated nitric oxide is efficiently combined with myoglobin to generate nitrosylmyoglobin, so that the meat products can obtain ideal red color equivalent to that treated by sodium nitrite even in low-temperature processing. The invention provides an efficient and feasible solution for replacing nitrite color development and improving the safety of meat products.

The application discloses umami peptides derived from Penaeus monodon and application thereof, and belongs to the technical field of bioactive peptides. The umami peptides are 11 in number, and the amino acid sequences are respectively ACWVPCEK, QRMMQ, DAKKACW, DRM, RMM, PDPDPT and the like, as shown in SEQ ID NO. 1-11. The umami peptides are applied in umami agents. The umami peptides are obtained from the myoglobin of Penaeus monodon by using a rapid screening technology, wherein the umami intensity of QRMMQ is the strongest, the umami threshold of PDPDPT is the lowest, and ACWVPCEK has a synergistic umami effect. The application enriches the umami peptide library of marine origin, improves the economic value of Penaeus monodon, and simultaneously studies key amino acids and an action mechanism of umami peptide binding with umami receptors, thereby providing a reference for studying the umami mechanism of umami peptides.

The application provides an adsorbent for removing myoglobin and a preparation method thereof, and the preparation method of the adsorbent for removing myoglobin comprises the following steps: mutual solubility of styrene monomers, polyvinyl cross-linking agents, halogen-containing monomers, initiators and pore-forming agents to obtain an oil phase, dissolving a dispersing agent in water to obtain an aqueous phase, mutual solubility of the aqueous phase and the oil phase to prepare an oil-in-water suspension, suspension polymerization reaction of the oil-in-water suspension to prepare a styrene macroporous resin containing halogenated hydrocarbons; and nucleophilic substitution reaction of the styrene macroporous resin containing halogenated hydrocarbons and an amino acid containing a benzene ring to graft the amino acid to the styrene macroporous resin to prepare the adsorbent. The removal rate and removal speed of the adsorbent prepared by the application to myoglobin are obviously improved, and when the adsorbent is applied to whole blood perfusion treatment, the adsorbent can quickly reduce the myoglobin concentration in the body of a patient in the early stage, and the whole blood perfusion treatment effect on the patient is improved.