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Reagent kit for detecting troponin I and preparation and use method thereof

A troponin and kit technology, which is applied in the field of protein detection methods and kits, can solve the problems that it is not suitable for small hospitals and medical points, cannot realize single-person detection, and cannot detect troponin, so as to achieve convenient promotion Use, fast detection speed, good specificity

Inactive Publication Date: 2012-09-19
王迎峰
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  • Abstract
  • Description
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  • Application Information

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Problems solved by technology

The detection sensitivity of enzyme-linked immunosorbent assay is high, but the operation process is relatively complicated, and the detection takes a long time; it needs special equipment such as microplate reader to cooperate with it, and the operator needs professional training; the cost of detection is relatively high. High, unable to achieve single-person detection
The chemiluminescence method has good precision and sensitivity, but requires special equipment, and the instruments and reagents are relatively expensive, so it is not suitable for small hospitals and medical centers
The operation of colloidal gold immunochromatography is simple, the time required for detection is short, no professional technicians are required to operate, and the detection cost is low. However, this method can only achieve qualitative or semi-quantitative detection, and the detection sensitivity is low. Troponin I

Method used

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  • Reagent kit for detecting troponin I and preparation and use method thereof
  • Reagent kit for detecting troponin I and preparation and use method thereof
  • Reagent kit for detecting troponin I and preparation and use method thereof

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Embodiment Construction

[0040] Specific embodiments of the present invention will be described below in conjunction with the accompanying drawings.

[0041]In one embodiment of the present invention, the kit includes a bottom plate, and sequentially connected sample pads, cross-linked product pads, link pads, nitrocellulose membranes, and absorbent paper are provided from one end to the other end of the bottom plate. The cross-linked material pad is coated with fluorescent silica nanoparticles-anti-troponin I monoclonal antibody cross-linked material, and the nitrocellulose membrane is provided with a detection strip and a quality control strip, and the detection strip is provided with The side close to the connecting pad contains anti-TnI monoclonal antibody, and the quality control strip is arranged on the side away from the connecting pad and contains goat anti-mouse IgG.

[0042] In a preferred embodiment of the present invention, with reference to the attached figure 1 In the method, the sample...

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Abstract

The invention relates to a reagent kit for detecting troponin I and a preparation and use method thereof. The reagent kit for detecting the troponin I comprises a base plate, a sample pad, a cross-linked substance pad, a connecting pad, a nitrocellulose membrane and absorbent paper which are connected sequentially are arranged on the base plate from one end to the other end, the cross-linked substance pad envelops cross-linked substances of fluorescent silica nanoparticle-anti-troponin I monoclonal antibodies, the nitrocellulose membrane is provided with a detection band and a quality control band, the detection band is arranged on one side close to the connecting pad and contains anti-troponin I monoclonal antibodies, and the quality control band is arranged on the other side away from the connecting pad and contains goat anti-mouse IgG (immunoglobulin G). The reagent kit for detecting the troponin I can achieve quantitative detection and high-sensitivity detection only by adopting a portable fluorescence detector, is simple in use, high in detection speed and low in cost and can be used for bedside diagnosis.

Description

technical field [0001] The invention relates to a protein detection method and a kit, in particular to a kit for detecting troponin I and a preparation method thereof, and also to a method for detecting troponin I with the kit. Background technique [0002] Myocardial injury markers refer to biochemical substances that can be measured in circulating blood and can sensitively and specifically reflect myocardial injury (abnormality) and its severity, so they can be used for screening of myocardial injury (abnormality) , diagnosis, assessment of prognosis and follow-up treatment. Under normal conditions, myocardial injury markers are mainly or exclusively present in the heart (myocardium), and are released in large quantities by the heart under cardiac or cardiovascular abnormalities. Cardiac markers mainly include myoglobin, troponin (cTn I and cTn T), creatine kinase isoenzyme (CK-MB), etc. Among them, cTn I and cTn T were unanimously rated as highly specific and highly sen...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N33/577G01N33/558G01N33/533
Inventor 雷文武
Owner 王迎峰
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