110 results about "Creatine" patented technology

The invention discloses bifidobacterium longum subsp.infantis capable of promoting bone growth and a metagen thereof, and belongs to the technical field of biology. The bifidobacterium longum subsp. Infantis CCFM1445 provided by the invention can promote the increase of bone volume fraction, femoral bone trabecula thickness and far-end growth plate thickness of mammals in a growth period, reduce the levels of serum bone resorption markers I-type collagen amino-terminal peptide and tartrate-resistant acid phosphatase, improve the levels of serum bone alkaline phosphatase and parathyroid hormone, and improve the clinical application value of the bifidobacterium longum subsp. Infantis CCFM1445. And the relative abundance of the other bacteria in the intestinal flora and the levels of creatine and indole-3-lactic acid are increased. The thermally inactivated bifidobacterium longum subsp. Infantis CCFM1445 can promote the increase of the volume fraction of femoral bones and the number of bone trabecula of malnourished mice and improve the N-terminal propeptide level of serum I-type procollagen. Therefore, the medicine or health care product prepared from the bifidobacterium longum subsp. Infantis CCFM1445 can be used for assisting in regulating bone metabolism and bone mass, and has a wide market prospect.

The invention belongs to the technical field of gestational diabetes diagnosis marker screening, and particularly relates to a biomarker for predicting or diagnosing gestational diabetes and application of the biomarker. The invention discloses a biomarker for predicting or diagnosing gestational diabetes mellitus, the biomarker is selected from one or more of hypoxanthine, cortisol, dihydrotachysterol and cycloleucine, and more preferably, the biomarker comprises hypoxanthine, cortisol, dihydrotachysterol, (3R, 4R)-D-ketolactone, creatine, cycloleucine and L-hydroorotic acid. The seven biomarkers disclosed by the invention can predict or diagnose gestational diabetes mellitus independently or jointly through plasma detection at the early pregnancy stage, effectively improve the early diagnosis rate of gestational diabetes mellitus and improve disease prognosis, and have clinical use and popularization values.

The invention belongs to the technical field of biological medicine, and particularly relates to a reagent for improving ovarian development and a preparation method and application thereof. The reagent for improving ovarian development is prepared by mixing the following raw materials in parts by weight: 20-30 parts of N-acetylcysteine; 5 to 10 parts of tranexamic acid; 3 to 8 parts of creatine; and 3000 parts of water. According to the reagent, the mRNA expression of PKD1P6 and follicle stimulating hormone receptors (FSHR) in ovarian tissues is up-regulated, the activity and functions of cumulus granular cells are enhanced, and apoptosis of the cumulus granular cells is inhibited, so that the effects of effectively promoting follicle development and inhibiting the apoptosis of the cumulus granular cells are achieved.

The disclosure is directed to methods related to creatinine, for example, a method for its safe administration to a human.

The invention relates to the technical field of biological medicine, in particular to application of a creatine related gene detection reagent in preparation of breast cancer prognosis products. The creatine related gene is an IGFBP1 gene and / or a TBC1D4 gene. According to the method, the expression levels of IGFBP1 and TBC1D4 genes in breast cancer patient samples are detected, a risk score is calculated by using a random survival forest (RSF) model, and the one-year, two-year and three-year survival rates of breast cancer patients are accurately predicted according to the risk score result. Meanwhile, the invention proves that IGFBP1 and TBC1D4 genes can be used as potential targets for breast cancer treatment, the IGFBP1 and fulvestrant as well as the TBC1D4 and vinblastine have stable binding capacity, and a new basis is provided for selection of individualized treatment medicines for breast cancer.

The invention provides a high-protein feed raw material and a preparation method thereof, and belongs to the field of protein feeds.The preparation method comprises the steps that animal-derived organs are selected, an animal-derived organ enzyme solution is obtained through a pretreatment means, and natural protein components in the animal-derived organ enzyme solution are reserved to serve as crude protein nutritional ingredients; step 2, adding a substrate and a methyl donor into the animal-derived visceral organ enzyme liquid, and generating guanidinoacetic acid and creatine through an enzymatic synthesis reaction to obtain a mixed liquid; and step 3, carrying out enzyme system inactivation, centrifugal filtration, drying and crushing on the mixed solution to obtain a finished product. According to the invention, guanidinoacetic acid and creatine are synthesized by in-situ catalysis of animal-derived visceral organ natural enzyme systems, and can be naturally fused with protein components, so that the problem of compatibility is avoided, and growth functional factors do not need to be added from an external source; natural enzyme systems of animal livers and kidneys are directly utilized, industrial enzymes do not need to be purchased, and the natural enzyme systems are more adaptive to a raw material system; protein, natural enzyme systems and mineral substances in the raw materials are synchronously utilized, so that one material has multiple purposes.

This invention provides a continuous method for preparing high-purity creatine monohydrate, comprising the following steps: a) adding chloroacetic acid aqueous solution dropwise to an aqueous methylamine solution, stirring at room temperature for 2 hours after the addition is complete; b) heating to 50-60°C and holding for 1 hour to remove most of the methylamine, then heating to 95°C to evaporate the methylamine aqueous solution for 1 hour; c) adding sodium hydroxide solution dropwise to the solution from step b) at 95°C, then heating to 105°C and concentrating under reduced pressure for 1.5 hours to approximately half the original volume, obtaining a creatine solution with a concentration of 30%-40%; d) cooling the solution from step c) to 40-60°C and adding a catalyst; e) drying to obtain pure creatine monohydrate. This invention reduces the methylamine equivalent, saving costs, and eliminates the need for purifying creatine for direct use in the next reaction; it can simultaneously lower the system reaction temperature and pH value to inhibit the formation of byproducts dicyandiamide and creatinine, and eliminates the need for debittering agents for purification and debittering, thus saving production costs.

The application relates to the technical field of component detection, and discloses an analysis and detection equipment based on a creatine sodium finished product, which comprises a detection mounting table, a telescopic assembly, a rotating assembly, a power assembly, a temporary storage assembly, a mixing pretreatment equipment and an analysis and detection equipment; the temporary storage assembly comprises a compression bag, a flexible liquid inlet, a one-way valve and a pressure relief valve; through the flexible compression bag and the one-way valve structure of the temporary storage assembly, the application realizes pressure-free injection of the sample, avoids poor liquid inlet or early liquid leakage caused by air pressure imbalance, adopts a servo motor and a speed reducer in cooperation with the power assembly, realizes accurate indexing in cooperation with the rotating assembly, ensures that the temporary storage assembly is stably aligned with the liquid outlet position, realizes controllable pressure liquid outlet through mechanical extrusion of the telescopic assembly, realizes stable sample delivery, non-deviation and uniform flow, and realizes smooth connection with the mixing pretreatment equipment, so that the creatine sodium detection precision and efficiency are greatly improved, and the structure is stable and reliable and easy to maintain.

It suppresses the decrease in creatine content over time. [Solution] The cosmetic composition contains zinc element (A), creatine (B), and creatinine (C).

This invention relates to an anti-wrinkle repair composition containing red camellia extract and its application. The anti-wrinkle repair composition of this invention comprises the following components in weight percentages: polyol 1: 1-25%; red camellia extract: 0.1-15%; creatine: 0.1-2%; carnosine: 0.01-0.5%; recombinant collagen: 0.01-5%; tetrahydrocurcumin inclusions: 1-20%; ergothioneine: 0.0001-1%; water: balance. This invention combats aging through multiple pathways and approaches, providing multi-dimensional skin anti-oxidation and anti-glycation, scavenging free radicals, promoting the production of type I collagen in the skin's extracellular matrix, and strengthening the skin barrier. This composition can be used as an active ingredient in various types of cosmetics, including aqueous solutions, lotions, creams, and shampoos.

The subject matter of the present invention is a creatine agglomerate with improved solubility characteristics in aqueous systems and improved handling, thus simplifying the intake of creatine. The agglomerate is characterized in that it contains 30 to 99.9 wt. % of ground creatine and / or ground creatine derivatives and / or ground creatine salts and 0.1 to 30 wt. % of a binder containing at least one oligosaccharide, in particular maltodextrin, based on the total weight of the agglomerate.

The invention belongs to the technical field of analytical chemistry, and discloses a method for detecting creatinine in a creatine product, which comprises the following steps: (1) precisely weighing a sample, adding a buffer solution, carrying out vortex oscillation dissolution, volume metering, shaking up, centrifuging, and passing through a water phase filter membrane to obtain a creatine test solution; (2) a creatine test solution is taken, a liquid chromatograph is adopted for liquid phase detection, and the liquid chromatography conditions are as follows: an SCX chromatographic column is adopted, an ammonium dihydrogen phosphate solution is taken as a mobile phase for isocratic elution, an ultraviolet detector is adopted, and the content of creatinine is obtained according to a liquid phase detection result. According to the method, the situation that the detection result of the creatinine in the creatine product is virtually high can be remarkably reduced, accurate quantification of the creatinine byproduct creatinine is achieved, and a more perfect data basis is provided for quality control of the creatine product.

This invention discloses a creatine amidoside hydrolase mutant for creatinine detection, along with methods for selecting, purifying, and detecting the mutant site. The mutant is a single-point mutation of the wild-type full-length amino acid sequence of the creatine amidoside hydrolase protein (Af-CRE) from Alcaligenes faecalis. This invention utilizes machine learning-assisted site-directed mutagenesis to create a creatine amidoside hydrolase mutant with enhanced activity, obtaining a mutant enzyme with improved activity and certain thermostability. Furthermore, it provides methods for purifying and detecting the mutant, overcoming the shortcomings of existing creatine amidoside hydrolases with poor activity that cannot meet the requirements for reagent applications. This lays the foundation for further expanding the industrial applications of creatine amidoside hydrolases.

Techniques includes measuring, using a creatine sensor, a creatine sensor current signal (ΔI2) of a first calibration solution (CS2). A creatine sensor sensitivity (Slope) for the creatine sensor is based on the creatine sensor current signal (ΔI2). The first calibration solution (CS2) has a known concentration of creatine (CR_CS2), a known concentration of creatinine (CREA_CS2), and a stable ratio of creatine to creatinine over a range of temperatures for a predefined shelf-life of the first calibration solution (CS2). The Techniques include measuring, using the creatine sensor, a measured creatine concentration (MCR_CS3) of a second calibration solution (CS3). The second calibration solution (CS3) has an initial known creatine concentration (CR_CS3), an initial known creatinine concentration (CREA_CS3), and an unstable ratio of creatine to creatinine that changes over the predefined shelf-life. Concentrations of creatine and creatinine in a sample are thereafter estimated.

The invention discloses a biosynthesis process of creatine monohydrate, and belongs to the technical field of bioengineering. The invention discloses a creatine monohydrate biosynthesis method, which is characterized in that sarcosine invertase is utilized to catalyze chloroacetic acid and monomethylamine to generate N-alkylation reaction so as to prepare sarcosine, and then creatine monohydrate is generated under the catalysis of the creatine invertase, the process is mild in reaction condition, the temperature is 25-37 DEG C, the pH is 6.0-8.0, the energy consumption is lower, the conversion efficiency is high, the environment is protected, and the economical efficiency is high. The invention provides a new synthesis method for sustainable synthesis of creatine monohydrate, and provides a new idea for industrial production of creatine monohydrate.

Provided is a combination of at least two of creatine, bifida ferment lysate and sechium edule fruit extract to repair DNA damage to the skin caused by light in the range 280-1400 nm. These agents are combined with UV filters and optionally one or more blue light and / or one or more IR reflector(s) in cosmetic compositions used to prevent sun-induced damage to the skin, in particular to prevent senolytic or senomorphic senescence and / or appearance of the visible signs of skin aging, in particular one or more of fine lines, wrinkles, age spots, skin dryness, thinning of the skin and loss of elasticity.

The invention belongs to the technical field of biology, and particularly relates to application of creatine in relieving heat stress injury of bovine mammary epithelial cells. It is innovatively found for the first time that creatine can improve the activity of bovine mammary epithelial cells suffering from heat stress, reduce the apoptosis amount, caused by heat stress, of the bovine mammary epithelial cells, inhibit expression of pro-apoptosis genes and promote expression of anti-apoptosis genes, and the method provides a new method for relieving or treating bovine heat stress injury, and has the advantages that the creatine is used for treating bovine heat stress injury; the composition can also be used as a protective agent for cattle to resist heat stress.

The invention provides a food composition for enhancing exercise tolerance as well as a preparation method and application of the food composition, and relates to the technical field of foods. The invention discloses a food composition. The composition is prepared from the following components in parts by weight: 6 to 16 parts of bovine blood oligopeptide, 10 to 20 parts of collagen, 1 to 3 parts of beta-carotene, 15 to 26 parts of radix codonopsis extract, 13 to 25 parts of pleurotus nebrodensis polysaccharide, 10 to 30 parts of isomaltooligosacharide, 5 to 10 parts of creatine, 4 to 10 parts of taurine, 0.2 to 0.6 part of vitamin B1, 0.2 to 0.6 part of vitamin B6, 5 to 15 parts of guarana extract, 4 to 12 parts of green tea extract and 4 to 12 parts of green coffee extract. 2-5 parts of L-carnitine, 5-12 parts of cordyceps militaris, 5-12 parts of red ginseng and 5-12 parts of lucid ganoderma. The obtained food composition has the effects of resisting fatigue and improving hypoxia tolerance, and is applied to preparation of food with the effects of enhancing exercise tolerance, resisting hypoxia and relieving fatigue.

This invention provides urolithin A cocrystals, their preparation methods, compositions, and applications. Specifically, this invention provides a urolithin A cocrystal with high bioavailability, wherein the urolithin A cocrystal is selected from the group consisting of: urolithin A-proline cocrystals, urolithin A-carnitine cocrystals, urolithin A-nicotinamide cocrystals, or urolithin A-creatine cocrystals. Compared to urolithin itself, the urolithin A cocrystals of this invention exhibit high stability, a significantly lower melting point, and significantly improved solubility and bioavailability. Furthermore, the preparation method is simple, easy to control, and has good reproducibility, allowing for stable acquisition of the target cocrystal. The composition containing the cocrystal, prepared by grinding, has high yield, low cost, and is suitable for large-scale production.

This application discloses the application of creatine in the preparation of drugs or feed additives for the treatment or prevention of pathogenic diarrhea in pigs. The drugs are creatine or creatine as an active ingredient with added excipients to form oral liquids, granules, tablets, or powders. The dosage is 30–300 mg / kg of pig body weight; or it can be directly added to feed at a creatine content of 0.05%–0.5% of the total weight of the pig feed. The drugs or feed additives provided in this application are highly safe, have no toxic side effects, and can effectively prevent and treat pathogenic diarrhea caused by one or more pathogens, including porcine epidemic diarrhea virus, porcine transmissible gastroenteritis virus, porcine rotavirus, and enterotoxigenic Escherichia coli. They improve the intestinal morphology and absorption function of sick pigs and reduce the morbidity and mortality of piglets.

The invention provides a method for analyzing a creatine monohydrate molecule hydrogen bond network and a vibration mode based on a terahertz spectrum. The method comprises the following steps: carrying out terahertz spectrum measurement on a creatine monohydrate sample at different temperatures (for example, the temperature is reduced from 300 K to 4.5 K) to obtain absorption spectrum data in a wave band of 1-14 THz; analyzing the spectral data, and dividing the absorption peaks according to a characteristic frequency of about 9.95 THz: defining the absorption peaks lower than the frequency as a hydrogen bond network vibration region, and defining the absorption peaks higher than the frequency as an intramolecular vibration region; the crystal structure of creatine monohydrate and the stability of the hydrogen bond network are evaluated and identified by observing a blue shift phenomenon of an absorption peak of a vibration area of the hydrogen bond network along with temperature reduction and local vibration characteristic peaks appearing at 6.58 THz and 7.97 THz at a specific temperature (such as lower than 200K). According to the method, a technical means for directly and nondestructively representing the dynamic characteristics of the creatine monohydrate crystal lattice is provided by establishing the corresponding relation among the temperature, the frequency and the vibration mode.

The invention discloses a three-enzyme-inorganic hybrid nanoflower as well as a preparation method and an application method thereof, and belongs to the technical field of enzyme immobilization. The three enzymes in the material are creatininase, creatinase and sarcosine oxidase, and the carrier is calcium phosphate. According to the three-enzyme-inorganic hybrid nanoflower, creatininase, creatinase and sarcosine oxidase are loaded on the same carrier, and the three-enzyme-inorganic hybrid nanoflower can be generated on the surface of a working electrode in situ and can also be directly dropwise added to the surface of the working electrode for electrochemical precise detection of creatinine. The method has the advantages of being simple in operation, green, environmentally friendly and the like, and the prepared three-enzyme-inorganic nanoflower material is high in stability and rapid in detection and has a good application prospect in the field of biosensing.

The invention relates to the technical field of creatine monohydrate production, in particular to a creatine monohydrate conveying and feeding device for creatine monohydrate food processing.The creatine monohydrate conveying and feeding device comprises a bearing base and a feeding assembly, and the feeding assembly comprises a height adjusting piece, a connecting base, a feeding hopper, a material guiding frame, a screening piece, two spiral feeding rods, two transmission shafts and a driving mechanism; a creatine monohydrate raw material is put into the feeding hopper, then impurity screening is conducted on the creatine monohydrate raw material through the screening piece, the creatine monohydrate raw material is dispersed in the screening process and then falls into the feeding hopper, the driving mechanism is controlled to drive the two transmission shafts to drive the two spiral feeding rods to rotate, and then the creatine monohydrate raw material is fed into the feeding hopper through the two rotating spiral feeding rods. And the screened creatine monohydrate raw material is dispersed and thrown out through the material guiding frame, so that the problem that the follow-up processing efficiency is poor due to the fact that the raw material is not dispersed in the feeding process of an existing feeding device and is excessively concentrated when being thrown into processing equipment is solved.

The utility model relates to the technical field of solution purification, and discloses a creatine monohydrate purification device capable of recycling mother liquor, which comprises a heat preservation enamel kettle shell, a stirring enamel kettle shell is fixedly connected in the heat preservation enamel kettle shell, and a driving end of a driving component is fixedly connected with a working gear I; a connecting shaft is rotatably connected to the interior of the gear protection block, a first transmission gear is fixedly connected to the exterior of the connecting shaft, a forward stirring shaft is fixedly connected to the interior of the first transmission gear, and a reverse stirring shaft is rotatably connected to the exterior of the forward stirring shaft. According to the utility model, the rotating motor is started to drive the working gear I, the working gear I drives the transmission gear I to rotate through the internal gear ring which is externally coupled and connected, then the transmission gear II which is connected with the bottom of the connecting shaft drives the working gear II to rotate, then the working gear II drives the forward stirring shaft, and the stirring fan blades are matched with the kettle bottom stirring block; and the solution is fully stirred.

This invention belongs to the field of organic synthesis technology, specifically relating to a creatine glycinate and its preparation method, comprising the following steps: pretreatment, drying creatine monohydrate, passing glycine through a 100-mesh sieve and storing it in a desiccator for later use, and selecting a reaction solvent; raw material dissolution, adding the reaction solvent to a reaction vessel, starting stirring and heating, adding creatine monohydrate, and stirring at a constant temperature until completely dissolved, finally obtaining a creatine solution; complexation reaction, adding glycine, increasing the stirring rate and heating after the addition is complete, maintaining the temperature for the complexation reaction, using staged stirring until the complexation reaction is complete; after the reaction is complete, using a gradient cooling method; crystallization and ripening, followed by constant temperature and low-speed stirring for 2 hours, then allowing the crystals to settle completely; after filtration, vacuum drying is performed to finally obtain creatine glycinate. This application solves the problems of uneven reaction, poor crystal quality, and low purity and yield in existing processes, achieving efficient and high-quality preparation of creatine glycinate.

This invention belongs to the field of organic synthesis technology, specifically relating to creatine nitrate and its preparation method, including the following steps: raw material pretreatment, pretreating creatine monohydrate; adding 600g of reaction solvent to a reaction vessel, stirring, adding 150g of purified creatine monohydrate from step 100, mixing evenly to obtain a reaction solution, and heating to 50-55℃; slowly adding 110g of 65% nitric acid dropwise to below the surface of the reaction solution, and after the addition is complete, maintaining the temperature at 50-55℃ for 2 hours to obtain a mixed solution; cooling the mixed solution to 0-5℃ at a cooling rate, adjusting the stirring rate, and stirring at a constant temperature for 2 hours to crystallize; filtering the above crystallization system, washing the filter cake 2-3 times with a cold solvent at 0-5℃; vacuum drying the filter cake, and sieving after drying to obtain the creatine nitrate product. This invention achieves efficient and stable preparation of creatine nitrate, significantly improving product yield and purity.

The present disclosure relates to the technical field of cosmetics, in particular to a high-temperature-resistant color-changing composition for cosmetics and application thereof, the high-temperature-resistant color-changing composition comprises the following components with mass percentage: 5-10% of transaminic acid or a derivative thereof, 0.3-0.8% of a saccharide compound, 0.5-1.4% of creatine or a derivative thereof, 0.3-1.2% of an imidazolinone compound, 0.3-0.4% of a lactam carboxylate, 0-1% of a pH regulator, and the balance is water.

The present invention provides a bovine sex-sorted sperm sorting solution mediated by a small molecule compound and its application. The sperm sorting solution comprises a sperm sorting protection solution and a sex control functional component. The sperm sorting protection solution contains sodium chloride, potassium chloride, calcium chloride dihydrate, sodium dihydrogen phosphate, glucose, sodium pyruvate, sodium bicarbonate, penicillin-streptomycin, and phenol red. The sex control functional component includes creatine and a 24e drug. The concentration of creatine is 450 μM-550 μM, and the concentration of the 24e drug is 0.27 μM-0.33 μM. The 24e drug is a pyrido [3,2-d]pyrimidine-based TLR7&8 dual agonist. The present invention can meet the large demand for sex-sorted semen in production. Moreover, the sorting process is carried out under the suitable conditions for sperm and does not require complex treatment of sperm. It takes less time for sorting and causes less damage to sperm.

The utility model relates to a kind of creatinine electrochemical detection test paper, belong to detection equipment technical field, especially creatinine electrochemical detection equipment technical field.The utility model is equipped with two groups of detection electrode with non-closed frame electrode structure and two independent blood tank channels opened by double-sided adhesive layer, the reaction of creatinine in blood sample and the reaction of creatine are independently carried out in two blood tanks, creatinine and creatine signals in blood sample can be detected simultaneously without mutual interference, so that the detection of creatinine is more accurate.In addition, the compact multilevel test paper structure design of the utility model not only reduces the volume of test paper, but also the design and layout of reasonable blood tank channel, escape gas port and detection electrode can facilitate the precise use of detector.

The utility model relates to the technical field of food processing, and discloses a granularity-adjustable creatine monohydrate special crusher which comprises a bottom plate, the top of the bottom plate is fixedly connected with a first supporting plate, the front side of the first supporting plate is fixedly connected with a fixing plate, the inner side of the first supporting plate is fixedly connected with a granularity-adjustable crushing mechanism, and the top of the bottom plate is fixedly connected with a second supporting plate. A first supporting plate is fixedly connected to the top of the fixing plate, a second supporting plate is fixedly connected to the top of the first supporting plate, a smashing pretreatment mechanism is fixedly connected to the inner side of the second supporting plate, the smashing mechanism comprises a smashing machine shell, the outer side of the smashing machine shell is fixedly connected to the inner side of the first supporting plate, and a driving assembly is fixedly connected to the top of the fixing plate. According to the creatine monohydrate crusher, the two connecting rods slide in the grooves formed in the connecting strips and the grooves formed in the crusher shell, the angle of the baffle is adjusted, the granularity of the crushed creatine monohydrate can be accurately controlled according to requirements, and the crusher can adapt to diversified production requirements.