251 results about "Creatine" patented technology

The invention discloses bifidobacterium longum subsp.infantis capable of promoting bone growth and a metagen thereof, and belongs to the technical field of biology. The bifidobacterium longum subsp. Infantis CCFM1445 provided by the invention can promote the increase of bone volume fraction, femoral bone trabecula thickness and far-end growth plate thickness of mammals in a growth period, reduce the levels of serum bone resorption markers I-type collagen amino-terminal peptide and tartrate-resistant acid phosphatase, improve the levels of serum bone alkaline phosphatase and parathyroid hormone, and improve the clinical application value of the bifidobacterium longum subsp. Infantis CCFM1445. And the relative abundance of the other bacteria in the intestinal flora and the levels of creatine and indole-3-lactic acid are increased. The thermally inactivated bifidobacterium longum subsp. Infantis CCFM1445 can promote the increase of the volume fraction of femoral bones and the number of bone trabecula of malnourished mice and improve the N-terminal propeptide level of serum I-type procollagen. Therefore, the medicine or health care product prepared from the bifidobacterium longum subsp. Infantis CCFM1445 can be used for assisting in regulating bone metabolism and bone mass, and has a wide market prospect.

Provided herein are ready-to-drink beverages at acidic and near neutral pH comprising creatine, at least one electrolyte.

The invention belongs to the technical field of gestational diabetes diagnosis marker screening, and particularly relates to a biomarker for predicting or diagnosing gestational diabetes and application of the biomarker. The invention discloses a biomarker for predicting or diagnosing gestational diabetes mellitus, the biomarker is selected from one or more of hypoxanthine, cortisol, dihydrotachysterol and cycloleucine, and more preferably, the biomarker comprises hypoxanthine, cortisol, dihydrotachysterol, (3R, 4R)-D-ketolactone, creatine, cycloleucine and L-hydroorotic acid. The seven biomarkers disclosed by the invention can predict or diagnose gestational diabetes mellitus independently or jointly through plasma detection at the early pregnancy stage, effectively improve the early diagnosis rate of gestational diabetes mellitus and improve disease prognosis, and have clinical use and popularization values.

According to the creatine-monohydrate pectin soft sweets and the preparation method, the low-amidation pectin is innovatively used in the field of the creatine-monohydrate pectin soft sweets through corresponding pretreatment, so that the problems caused by reaction and decomposition of an amphoteric compound, namely creatine monohydrate, in an original high-ester pectin system are solved; meanwhile, the problem that conventional low-amidation pectin cannot be gelatinized during pouring is solved, the low-amidation pectin and the creatine monohydrate are innovatively combined, and a gel system capable of containing a high-concentration creatine monohydrate compound is prepared. And meanwhile, by adopting a sugar-free formula technology, the requirement that sports people need to supplement creatine monohydrate but do not wish to intake sugar is met.

Provided herein are maturation media and methods for maturing early embryonic human cardiac organoids to mature human cardiac organoids. The maturation medium comprises a cell growth medium comprising a medium supplement comprising: one or more fatty acids, a triiodothyronine (T3) growth hormone, insulin, one or more antioxidants, a sugar, and carnitine; one or more additional fatty acids; additional carnitine or creatine; and a further T3 growth hormone. The maturation medium may also include one or more additional sugars, additional antioxidants, and growth factors. The methods include contacting an early embryonic human cardiac organoid with one or more maturation media to produce a mature human cardiac organoid.

The invention discloses a metabolic biomarker combination for diagnosing depression. The metabolic biomarker combination comprises proline, betaine, alanine, tryptophan, kynurenine, 5-hydroxytryptamine, creatine, succinic acid, taurine and 2-hydroxybutyric acid. Based on a metabolic biomarker combination, an optimal diagnosis model is screened through a machine learning model algorithm, and the kit for depression diagnosis is constructed.

The invention discloses a preparation method of a dry chemical reagent for creatine content determination. The preparation method comprises the following steps: S1, preparing a buffer reagent and a protective component; s2, dissolving the biological enzyme and the chromophoric substrate to form a dissolving reagent; s3, coating by using a biological film-forming material to form a coating reagent; s4, coating and printing the coating reagent in the pore channel of the specified bottom plate base material in a volume quantitative manner by virtue of equipment; the equipment comprises a single-channel type pipettor, a multi-channel type pipettor, a continuous multi-gear distributor or automatic dot printing equipment. S5, placing the specified bottom plate base material printed with the coating reagent in forced air drying equipment or drying equipment with the same efficiency; and S6, measuring the moisture content after drying and film forming, and judging whether substances containing biologically active enzymes and substrates in the reagent are completely locked to the position of the net-shaped cavernous body structure of the biological film forming material or not based on the moisture content. The invention further discloses application of the dry chemical reagent for determining the creatine content, and the dry chemical reagent is applied to a sample formed by urine, serum or tissue.

The utility model discloses a creatine monohydrate drying and smashing device which comprises a drying box, the side surface of the drying box is fixedly connected with a controller, the side surface of the drying box is fixedly connected with a first motor, the output end of the first motor is fixedly connected with a lead screw, the side surface of the lead screw is in threaded connection with a sleeve, and the sleeve is fixedly connected with a motor. And the side surface of the sleeve is fixedly connected with a limiting block, the side surface of the sleeve is fixedly connected with a scraping plate, and the side inner wall of the drying box is fixedly connected with a bearing plate. According to the structure, through cooperation of the drying box, the bearing plate, the access door, the insertion plate, the first motor, the lead screw and the scraper, when powder is taken out after being dried, the powder can be pushed out in the scraper moving process without opening the box door, waste caused by hot air loss when the box door is opened is avoided, and meanwhile the situation that hot air burns a human body can be effectively reduced; and due to the existence of the filter screen, the materials can fall down after being completely crushed, and different particle crushing degrees are avoided.

The invention provides an organoid basic culture medium and a method for maintaining and promoting organoid growth, and the organoid basic culture medium comprises a basic culture medium and an additive, the additives comprise taurine sodium 3-hydroxybutyrate, taurine, sodium hypoxanthate, creatinine, L-ornithine, urea, L-sodium lactate, putrescine dihydrochloride, anhydrous citric acid, ethanolamine, N-acetylglycine, alpha ketoglutaric acid, formic acid, malic acid, malonic acid, succinic acid, uric acid, ammonium metavanadate, manganese dichloride, sodium selenite, creatine, glycerol and uridine. Anhydrous sodium acetate and para aminobenzoic acid. When the organ-like basic culture medium is used for treating the organ-like, the activity of the organ-like can be effectively maintained, and basic nutrition and metabolism requirements of the organ-like can be provided, so that the organ-like has the advantages of high activity, fast proliferation and long-term stable passage, and is suitable for various types of organs and wide in application.

The invention provides a preparation method of creatine ethyl ester hydrochloride, and belongs to the technical field of organic synthesis. The method comprises the following steps: dissolving creatine in absolute ethyl alcohol, adding hydrochloric acid to adjust the pH value to 2.5-3.5, then adding a copper-based complex catalyst at 60-80 DEG C, reacting for 0.5-2 hours, and after the reaction is completed, performing post-treatment to obtain a product. The copper-based complex catalyst is obtained by reacting CuSO4. 5H2O with 2, 2 '-dipyridyl in absolute ethyl alcohol to prepare a precursor, loading the precursor on a silicon dioxide carrier with a high specific surface area, and carrying out functionalization treatment through 3-aminopropyltriethoxysilane. The structure of the catalyst is optimized, the selectivity and purity of the product are remarkably improved, and high-purity and high-yield creatine ethyl ester hydrochloride is prepared. In addition, the catalyst is easy to recover and reuse, the production cost is reduced, and the method is suitable for industrial production.

The invention discloses a magnetic resonance brown fat activity imaging method, system and equipment and a storage medium. The method comprises the following steps: acquiring a chemical exchange saturation transfer image of a shoulder and neck region of a human body; performing normalization processing on the acquired chemical exchange saturation transfer image to obtain an original Z-spectrum image, performing primary three-pool Lorentz curve fitting on the original Z-spectrum image, finding out the position of a water peak, and correcting the main magnetic field nonuniformity of the original Z-spectrum image; and carrying out two-pool Lorentz curve fitting on the Z-spectrum image after main magnetic field non-uniformity correction again, extracting a creatine signal contained in the Z-spectrum image, and taking the creatine signal as an image quantitative index for reflecting the brown fat activity level to realize brown fat activity imaging. The method is non-invasive, non-radiative and low in cost, can be used in women and children, and is suitable for large-queue and repeated observation research.

The invention relates to the field of preparation of guanidine or derivatives thereof, and particularly discloses high-purity creatine monohydrate and a preparation method thereof. The preparation method comprises the following steps: sequentially carrying out condensation reaction, acidolysis reaction and hydrolysis reaction on dimethyl sulfate and urea, cooling, crystallizing and filtering to obtain O-methylisourea hydrogen sulfate, and then reacting a sarcosine salt aqueous solution with the O-methylisourea hydrogen sulfate under the conditions that the temperature is 45-65 DEG C and the pH value is 10-12 to obtain the sarcosine salt. And performing post-treatment to obtain the high-purity creatine monohydrate. The preparation method has the advantages of being low in raw material cost, easy to transport and store and rich in source, byproducts in the reaction process are few, danger is low, the yield and purity are high, the industrial requirement is met, and the preparation method is suitable for large-scale industrial production.

The present invention discloses the use of tyrosol as an enhancer of the physiological effects of creatine and its application in continuous high-intensity exercise. The enhancement of physiological effects includes, but is not limited to, improves muscle endurance, reduces decline in muscle strength, enhances utilization of creatine by muscles, stimulation of the endurance-enhancing effects of creatine, reduces the decline of endurance levels, accelerates resynthesis of phosphocreatine, increases phosphocreatine levels, improves resistance performance, and enhances endurance exercise performance. Particularly, muscle endurance after continuous high-intensity exercise does not decrease or does not decrease significantly.

The invention belongs to the field of industrial application of microalgae, and provides a method for improving astaxanthin produced by haematococcus pluvialis. Comprising the following steps: 1, culturing haematococcus pluvialis to a logarithmic phase; 2, the haematococcus pluvialis is diluted to a proper concentration, and a seed solution for producing astaxanthin from the haematococcus pluvialis is prepared; 3, respectively adding six pre-prepared inducers including creatine, glycine, 6-aminocaproic acid, glutamic acid, L-hydroxyproline and L-cysteine into the prepared haematococcus pluvialis liquid for induction; and 4, continuously filling sterile air into the haematococcus pluvialis liquid added with the inducer in a photobioreactor, culturing, and inducing haematococcus pluvialis cells to accumulate astaxanthin. The invention discloses a method for improving astaxanthin produced by haematococcus pluvialis. The haematococcus pluvialis can be promoted to accumulate a large amount of astaxanthin, and the production cycle of the astaxanthin is shortened. The method is easy to operate, high in efficiency, low in cost and completely suitable for industrial culture of haematococcus pluvialis.

The present invention relates to an acne removing composition comprising salicylic acid and creatine wherein the weight ratio of creatine to salicylic acid is from about 1: 6 to 6: 4, preferably from about 1: 5 to 5: 4, more preferably from about 1: 4 to 4: 4, still more preferably from about 1: 3 to 3: 4.

The invention discloses an application of creatine in improving the development rate of an in-vitro blastocyst of a sheep oocyte. The concentration of the creatine is 0.5 mmol / L. The creatine is added into an in-vitro maturation system and an in-vitro embryo culture system. The key metabolite creatine is analyzed and screened based on metabonomics of sheep oocytes, early embryos, follicular fluid and fallopian tube fluid. On the basis, the development regulation effect of the creatine on the oocytes and early embryos is researched, the in-vitro blastocyst development rate of the sheep oocytes can be remarkably increased by adding the creatine into an in-vitro maturation system and an in-vitro embryo culture system of the sheep oocytes, and it is proved that the creatine can serve as an additive of the in-vitro culture system; and the creatine is a key metabolite contained in the development microenvironment and is friendly to oocyte and embryo development.

The invention relates to a composition with effects of controlling oil and removing dandruff and application of the composition. The composition with the effects of controlling oil and removing dandruff comprises sophorolipid, myristicamidopropyl PG-dimethyl ammonium chloride phosphate, creatine, a phellinus linteus extract, a hop extract and sodium chloride. According to the composition, the sophorolipid, the myristicamidopropyl PG-dimethyl ammonium chloride phosphate, the creatine, the phellinus linteus extract, the humulus lupulus extract and the sodium chloride are matched for use, the synergistic interaction effect is achieved, the hair cleaning effect can be achieved, meanwhile, healthy water-oil balance is provided for scalp, and the hair care effect is achieved. The completeness of the scalp barrier is favorably kept, so that the sebum secretion of the scalp is better reduced, and the formation of dandruff is reduced.

The invention discloses an application of creatine in preparation of a medicine or a feed additive for treating or preventing pig pathogenic diarrhea, the medicine is creatine or is prepared into oral liquid, granules, tablets and powder by taking the creatine as an active ingredient and adding auxiliary materials, and the use dosage is that the content of the creatine is 30-300mg / kg of pig body weight; or the feed additive is directly added into the feed, and the adding amount is that the content of creatine accounts for 0.05-0.5% of the total weight of the pig feed. The medicine or the feed additive provided by the invention is high in safety and free from toxic and side effects, can effectively prevent and treat pathogenic diarrhea caused by infection of one or more pathogens of porcine epidemic diarrhea virus, porcine transmissible gastroenteritis virus, porcine rotavirus and enterotoxigenic escherichia coli, improves the intestinal morphological structure and the absorption function of diseased pigs, and improves the immunity of the pigs. The morbidity and mortality of piglets are reduced.

The invention belongs to the technical field of biological medicine, and particularly relates to a reagent for improving ovarian development and a preparation method and application thereof. The reagent for improving ovarian development is prepared by mixing the following raw materials in parts by weight: 20-30 parts of N-acetylcysteine; 5 to 10 parts of tranexamic acid; 3 to 8 parts of creatine; and 3000 parts of water. According to the reagent, the mRNA expression of PKD1P6 and follicle stimulating hormone receptors (FSHR) in ovarian tissues is up-regulated, the activity and functions of cumulus granular cells are enhanced, and apoptosis of the cumulus granular cells is inhibited, so that the effects of effectively promoting follicle development and inhibiting the apoptosis of the cumulus granular cells are achieved.

The present invention relates to a creatine agglomerate having improved dissolution properties in an aqueous system and having improved handling properties, thereby making creatine readily ingested. The agglomerate is characterized in that it comprises, based on the total weight of the agglomerate, 30 to 99.9% by weight of ground creatine and / or ground creatine derivatives and / or ground creatine salts, and 0.1 to 30% by weight of a binder comprising at least one oligosaccharide, in particular maltodextrin.

The disclosure is directed to methods related to creatinine, for example, a method for its safe administration to a human.

Devices, kits, and methods for testing and monitoring creatine phosphokinase (CPK) in biological samples are provided.

To provide a method for stabilizing a creatinine-containing substance in which, while a calibration substance of creatinine measurement in a sample, a standard substance of creatinine measurement in a sample or a reference substance of accuracy control of creatinine measurement in a sample, or the like that is a creatinine-containing substance to be used in creatinine measurement in a sample, needs to be in a liquid state during use, a concentration of creatinine varies with time in a creatinine-containing substance in the liquid state, and therefore the stability has been inadequate.SOLUTION: In a creatinine-containing substance of a liquid state and a refrigerated condition, in which a solvent is blood serum, blood plasma, or a protein-containing solution, creatine is made to co-exist with creatinine, and further, made to co-exist such that a molar ratio obtained by dividing the creatine concentration (mol / L) by the creatinine concentration (mol / L) falls in the range of 2.0-2.8.SELECTED DRAWING: None

The invention relates to the technical field of biological medicine, in particular to application of a creatine related gene detection reagent in preparation of breast cancer prognosis products. The creatine related gene is an IGFBP1 gene and / or a TBC1D4 gene. According to the method, the expression levels of IGFBP1 and TBC1D4 genes in breast cancer patient samples are detected, a risk score is calculated by using a random survival forest (RSF) model, and the one-year, two-year and three-year survival rates of breast cancer patients are accurately predicted according to the risk score result. Meanwhile, the invention proves that IGFBP1 and TBC1D4 genes can be used as potential targets for breast cancer treatment, the IGFBP1 and fulvestrant as well as the TBC1D4 and vinblastine have stable binding capacity, and a new basis is provided for selection of individualized treatment medicines for breast cancer.

The present invention provides a method for improving exercise performance or preventing or relieving fatigue in mammal, including administration to the mammal a composition including: (i) an effective amount of BAIBA, or a pharmaceutically acceptable salt, acid, ester, polymer, analog or derivative thereof; and (ii) an effective amount of creatine. The present invention also provides a composition, including (i) an effective amount of BAIBA, or a pharmaceutically acceptable salt, acid, ester, polymer, analog or derivative thereof; and (ii) an effective amount of creatine, for improving exercise performance or preventing or relieving fatigue in a mammal. The methods and compositions of the invention can be used for enhancing muscle strength, increasing muscle content, improving muscle endurance; preventing body fatigue after exercise, accelerating recovery after exercise.

The invention provides a high-protein feed raw material and a preparation method thereof, and belongs to the field of protein feeds.The preparation method comprises the steps that animal-derived organs are selected, an animal-derived organ enzyme solution is obtained through a pretreatment means, and natural protein components in the animal-derived organ enzyme solution are reserved to serve as crude protein nutritional ingredients; step 2, adding a substrate and a methyl donor into the animal-derived visceral organ enzyme liquid, and generating guanidinoacetic acid and creatine through an enzymatic synthesis reaction to obtain a mixed liquid; and step 3, carrying out enzyme system inactivation, centrifugal filtration, drying and crushing on the mixed solution to obtain a finished product. According to the invention, guanidinoacetic acid and creatine are synthesized by in-situ catalysis of animal-derived visceral organ natural enzyme systems, and can be naturally fused with protein components, so that the problem of compatibility is avoided, and growth functional factors do not need to be added from an external source; natural enzyme systems of animal livers and kidneys are directly utilized, industrial enzymes do not need to be purchased, and the natural enzyme systems are more adaptive to a raw material system; protein, natural enzyme systems and mineral substances in the raw materials are synchronously utilized, so that one material has multiple purposes.

This invention provides a continuous method for preparing high-purity creatine monohydrate, comprising the following steps: a) adding chloroacetic acid aqueous solution dropwise to an aqueous methylamine solution, stirring at room temperature for 2 hours after the addition is complete; b) heating to 50-60°C and holding for 1 hour to remove most of the methylamine, then heating to 95°C to evaporate the methylamine aqueous solution for 1 hour; c) adding sodium hydroxide solution dropwise to the solution from step b) at 95°C, then heating to 105°C and concentrating under reduced pressure for 1.5 hours to approximately half the original volume, obtaining a creatine solution with a concentration of 30%-40%; d) cooling the solution from step c) to 40-60°C and adding a catalyst; e) drying to obtain pure creatine monohydrate. This invention reduces the methylamine equivalent, saving costs, and eliminates the need for purifying creatine for direct use in the next reaction; it can simultaneously lower the system reaction temperature and pH value to inhibit the formation of byproducts dicyandiamide and creatinine, and eliminates the need for debittering agents for purification and debittering, thus saving production costs.

The present invention provides an electrode for electrochemical detection of creatinine, including an electrode material base layer; the outer surface of the electrode material base layer is covered with an electron mediator enhancement layer; the outer surface of the electron mediator enhancement layer is fixed with an enzyme composition that can react with creatinine to generate hydrogen peroxide; the electrode material base layer is composed of carbon paste or graphite containing Prussian blue; the enzyme composition is composed of creatinase, creatinase and sarcosine oxidase in a ratio of 3.75-6.25:1.25-2:0.75-1.25. The present invention also provides a test paper and a biosensor based on the electrode. The present invention also provides a preparation method for the electrode and the test paper, and a method for detecting creatinine levels using the biosensor. The test paper of the present invention can be used in a variety of portable and fast small electrochemical detection equipment, and can achieve rapid and convenient clinical detection or home monitoring of blood and urine creatinine.

The application relates to the technical field of component detection, and discloses an analysis and detection equipment based on a creatine sodium finished product, which comprises a detection mounting table, a telescopic assembly, a rotating assembly, a power assembly, a temporary storage assembly, a mixing pretreatment equipment and an analysis and detection equipment; the temporary storage assembly comprises a compression bag, a flexible liquid inlet, a one-way valve and a pressure relief valve; through the flexible compression bag and the one-way valve structure of the temporary storage assembly, the application realizes pressure-free injection of the sample, avoids poor liquid inlet or early liquid leakage caused by air pressure imbalance, adopts a servo motor and a speed reducer in cooperation with the power assembly, realizes accurate indexing in cooperation with the rotating assembly, ensures that the temporary storage assembly is stably aligned with the liquid outlet position, realizes controllable pressure liquid outlet through mechanical extrusion of the telescopic assembly, realizes stable sample delivery, non-deviation and uniform flow, and realizes smooth connection with the mixing pretreatment equipment, so that the creatine sodium detection precision and efficiency are greatly improved, and the structure is stable and reliable and easy to maintain.

It suppresses the decrease in creatine content over time. [Solution] The cosmetic composition contains zinc element (A), creatine (B), and creatinine (C).