56 results about "Hydroxyethyl starch" patented technology

The invention discloses a chitosan modified attapulgite composite material for sewage treatment as well as a preparation method and application thereof, and relates to the technical field of chitosan modified composite materials and sewage treatment agents. The chitosan modified attapulgite composite material is obtained by loading a glutaraldehyde cross-linked sulfonated chitosan-starch copolymer on the surface of attapulgite. In the preparation process of the glutaraldehyde cross-linked sulfonated chitosan-starch copolymer, sulfonated chitosan is firstly prepared, then the sulfonated chitosan is cross-linked by glutaraldehyde, and then the sulfonated chitosan is cross-linked with hydroxyethyl starch to obtain the glutaraldehyde cross-linked sulfonated chitosan-starch copolymer. The chitosan modified attapulgite composite material for sewage treatment has a good flocculation effect on impurities in sewage, can effectively adsorb heavy metal impurities in the sewage, has excellent low-temperature resistance, can effectively precipitate sediment particles in river sewage, and can be used for treating the sewage. And high sewage treatment efficiency can also be ensured in a low-temperature environment.

The invention relates to the technical field of organ transplantation, and discloses organ preserving fluid, application and organ dynamic supercooling perfusion equipment. The organ preserving fluid is prepared from ribose, glucose, mannitol, lidocaine, adenosine, a high-molecular polymer, L-proline, trehalose, inorganic salt and organic salt. The high-molecular polymer comprises one of hydroxyethyl starch, polyethylene glycol and albumin. According to the organ preserving fluid disclosed by the invention, the freezing point of the perfusate is reduced through the micromolecular cryoprotectant, and meanwhile, the micromolecular cryoprotectant and the high-molecular polymer jointly inhibit formation and growth of ice crystals; the problem of liquid preservation at the temperature below zero is solved from the two dimensions of physics (reducing the freezing point and inhibiting ice crystals) and biology (maintaining cell viability), and feasibility is provided for long-time low-temperature transfer and transplantation of organs. The preserving fluid can be used for static preservation or perfusion preservation of various organs such as kidney, liver, heart, pancreas, lung, small intestine, skin and the like.

The invention discloses a method for calculating the molar substitution degree of hydroxyethyl starch by applying a nuclear magnetic resonance carbon spectrum, and belongs to the technical field of analysis and detection. The method comprises the following steps: carrying out nuclear magnetic resonance test on a sample to be detected to obtain a nuclear magnetic resonance 13C spectrum, and determining the molar substitution degree of hydroxyethyl starch according to the peak integral ratio of hydroxyethyl alpha carbon or beta carbon in hydroxyethyl starch glucose to glucose 1-position heterogenous carbon; compared with a traditional Morgan method and a traditional gas chromatography method, the method has the advantages that the operation process is simplified, time and labor are saved, the consumption of experimental consumables and reagents is greatly reduced, the molar substitution degree can be intuitively calculated on the basis of not damaging the structure of a sample, and the method is simpler, more convenient, high in accuracy, simple and convenient to operate and high in detection speed; the method can be used for detecting the molar substitution degree of hydroxyethyl starch.

The invention discloses a cell freezing medium and application thereof, and belongs to the technical field of biology, the cell freezing medium comprises the following components: 5-10% of DMSO, 50-75% of dextran 40 glucose injection, 15-45% of human serum albumin, 2.5-5% of compound electrolyte solution, 5-20% of hydroxyethyl starch electrolyte injection, 10-40ng / mL of vitamin C, and 10-40ng / mL of vitamin B6.

The invention provides a cryopreservation agent and method suitable for a cell-hydrogel precursor system, the cryopreservation agent comprises 2 vol%-11 vol% of a permeable cryopreservation agent, a non-permeable cryopreservation agent, 1 vol%-3 vol% of a hydrogel precursor and a solvent, and does not comprise dimethyl sulfoxide and serum; the permeable cryoprotectant is at least two of glycerol, propylene glycol, ethylene glycol and 3-OMG, and the volume fraction of each of the glycerol, the propylene glycol, the ethylene glycol and the 3-OMG is less than 6%; the non-permeable cryoprotectant comprises 0.1 to 0.6 M of non-permeable micromolecular carbohydrates and 1 to 2 vol% of non-permeable macromolecular substances; the impermeable high-molecular substance is at least two of dextran, polyethylene glycol, polyvinyl alcohol, hydroxyethyl starch and antifreeze protein, and the volume fraction of each of the dextran, the polyethylene glycol, the polyvinyl alcohol, the hydroxyethyl starch and the antifreeze protein is less than 1.5%; the cryopreservation agent is suitable for cryopreservation of a cell-hydrogel precursor system and can be directly injected into gel after resuscitation.

The present invention provides a cryopreservation solution for Koi herpesvirus, as well as its preparation method and application, relating to the field of virus preservation technology. The solution comprises 2-8 w / v% hydroxyethyl starch, 3-5 w / v% sucrose, and 1-3 w / v% bovine serum albumin. The Koi herpesvirus has an activity of ≥90% after resuscitation, can withstand five freeze-thaw cycles, can be stably frozen at -20°C, and has a shelf life of up to two years. This solution solves the technical problems in the prior art, such as the need to store cryopreservation solutions at ultra-low temperatures, the risk of osmotic pressure imbalance and viral envelope rupture, and low activity after cryopreservation and resuscitation.

The invention relates to the technical field of freeze-drying preparations, in particular to a plasma protective agent, a freeze-drying method for keeping the pH of plasma and freeze-dried plasma, and the plasma protective agent comprises the following components: an instant protective agent which comprises 5mg / ml of glycine; the feed additive is prepared from sodium dihydrogen phosphate, trehalose, hydroxyethyl starch, polyethylene glycol, mannitol, taurine, tryptophan lysine or glutamic acid. The sustained-release protective agent comprises antioxidant components encapsulated by lipidosome, wherein the antioxidant components comprise glutathione, adenosine and vitamin C. The invention also discloses a preparation method of the sustained-release protective agent. The novel protective agent vitamin C is adopted, the plasma pH adjusting operation is remarkably simplified, and the vitamin C is composed of multiple components with different functions, so that the stability of key components such as blood coagulation factors in plasma is greatly improved.

The invention discloses a cryopreservation method and application of an NK cell cryopreservation solution, and belongs to the technical field of cell cryopreservation. The NK cell cryopreservation solution comprises DMSO, a PBS buffer solution and a hydroxyethyl starch solution; the volume fraction of the DMSO in the NK cell freezing medium is 2.8 to 3.2 percent; the cryopreservation method of the NK cell cryopreservation liquid comprises the following steps: culturing activated NK cells by using a complete culture medium added with IL-15 and IL-18, centrifuging, cleaning, preparing the NK cell cryopreservation liquid, re-suspending to a preset cell concentration by using the NK cell cryopreservation liquid, cooling, freezing, and preserving in liquid nitrogen. The NK cell cryopreservation liquid used in the invention is a universal NK cell cryopreservation liquid, and the cryopreservation method provided by the invention has a good cryopreservation effect and can be used instantly.

The present invention discloses a chitosan-modified attapulgite composite material for sewage treatment, its preparation method and application, which relate to the technical fields of chitosan-modified composite materials and sewage treatment agents. The chitosan-modified attapulgite composite material is obtained by loading a glutaraldehyde-crosslinked sulfonated chitosan-starch copolymer on the surface of attapulgite. In the preparation process of the glutaraldehyde-crosslinked sulfonated chitosan-starch copolymer, sulfonated chitosan is first prepared, then sulfonated chitosan is crosslinked with glutaraldehyde, and then crosslinked with hydroxyethyl starch. The chitosan-modified attapulgite composite material for sewage treatment of the present invention has a good flocculation effect on impurities in sewage, can effectively adsorb heavy metal impurities in sewage, and has excellent low-temperature resistance. It can effectively precipitate sediment particles in river sewage and ensure a high sewage treatment efficiency even in a low-temperature environment.

The invention provides a bovine embryo refrigerated preservation solution. The bovine embryo refrigerated preservation solution is prepared from hydroxyethyl starch, dimethyl sulfoxide, fetal calf serum, cane sugar, cepharanthine and deionized water. The ratio of the components is further optimized, and the components comprise 5% of hydroxyethyl starch, 5% of dimethyl sulfoxide, 55% of fetal calf serum, 37% of deionized water, 2% of cane sugar and 1% of cepharanthine. Under the optimal raw material ratio condition that the concentration of cepharanthine is 600 [mu] mol / L and the concentration of cane sugar is 2 mol / L, cepharanthine is added into the bovine embryo preserving fluid, through a triple synergistic mechanism of oxidation resistance, apoptosis resistance and immunoregulation, the defect of biochemical damage protection in the prior art is overcome, and full-period protection is provided for embryos.

The invention discloses a drying protective agent for nuclear signal cell climbing slide. The drying protective agent comprises the following components: 80 g / L of trehalose, 30 g / L of sucrose, 5% of mannitol by volume fraction, 0.5% of TritonX-100 by volume fraction, 30 g / L of hydroxyethyl starch, 40 g / L of BSA, 40 g / L of sodium carboxymethyl cellulose, 40 g / L of polyvinylpyrrolidone, 10 g / L of cysteine and 5-7 g / L of sodium citrate. In the protective agent, TritonX-100 can pass through a cell membrane to release nucleoprotein, sample or antibody incubation can be directly carried out without permeation in subsequent detection, and other effective components can be loaded into cells to play a good stabilizing and protecting role in the cell drying process, so that morphological variation and damage of the cells are effectively avoided, antigen protein conformation is maintained, and the cell quality is improved. Therefore, the stability of the dried cells is improved.

The invention provides a low-temperature cryopreservation protective solution for koi herpesvirus as well as a preparation method and application thereof, and relates to the technical field of virus preservation, the low-temperature cryopreservation protective solution comprises 2-8w / v% of hydroxyethyl starch, 3-5w / v% of sucrose and 1-3w / v% of bovine serum albumin, the activity of the koi herpesvirus after recovery is greater than or equal to 90%, the koi herpesvirus can tolerate five freeze-thaw cycles, the koi herpesvirus can be stably frozen at-20 DEG C, and the storage life is prolonged to 2 years. The technical problems that in the prior art, cryopreservation liquid needs to be stored under the ultralow temperature condition, osmotic pressure is unbalanced, virus envelopes are broken, and the activity is low after cryopreservation recovery are solved.

The invention belongs to the technical field of biological medicine, and particularly relates to a normal-temperature cell protective agent, a normal-temperature cell protective solution and application thereof. The normal-temperature cell protective agent is prepared from human serum albumin, trehalose, 6-amino nicotinamide, hydroxyethyl starch and compound electrolyte. The normal-temperature cell preserving fluid comprises the normal-temperature cell protecting agent and a physiologically acceptable carrier. According to the normal-temperature cell preservation solution, normal-temperature transportation of stem cells can be achieved, the motility rate of the stem cells is not affected by external temperature change in a short time (within 3 days), the cells are well protected within the temperature range of 4-25 DEG C, the cell survival rate is high, the cell survival rate can reach 97% or above within 72 h, and the normal-temperature cell preservation solution is remarkably superior to an existing cell preservation solution.

The invention relates to a modified hydroxyethyl starch. According to the invention, the hydroxyethyl starch carries a heptonic acid residue on at least one of its termini. The invention further relates to a method for preparation such a starch and to novel uses of such a starch.

The invention discloses a hepatocyte cryopreservation solution which comprises a first cryopreservation mixed solution, a second cryopreservation mixed solution and fetal calf serum, the first cryopreservation mixed solution is prepared from a Leibovitz's L-15 culture medium, lactobionic acid, raffinose, reduced glutathione, ademetionine, curcumin, bicyclol, ursodesoxycholic acid, silymarin and glycyrrhizic acid; the second cryopreservation mixed solution comprises cane sugar, trehalose, hydroxyethyl starch and polyene phosphatidylcholine. On the other hand, the invention discloses a cryopreservation method and a recovery method of primary mouse hepatocytes based on the hepatocyte cryopreservation liquid. The cryopreservation method comprises the following steps: pre-culturing, re-suspending, slowly dropwise adding DMSO (dimethylsulfoxide) and cooling and cryopreserving step by step. The resuscitation method comprises the steps of rapid unfreezing, centrifugal purification and resuscitation culture. The liver cell cryopreservation liquid and the cryopreservation and resuscitation method can play roles in regulating osmotic pressure, inhibiting ice crystal formation, stabilizing cell membrane structure, resisting oxidation, promoting protein synthesis and the like, and the resuscitated cells are high in survival rate and strong in adherence capability and functional activity.

The utility model relates to a hydroxyethyl starch closed etherification precise control system, and belongs to the technical field of hydroxyethyl starch processing. The system comprises a nitrogen storage tank, an ethylene oxide storage tank and an etherification tank, one end of the first connecting pipeline is communicated with the nitrogen storage tank, the other end of the first connecting pipeline is communicated with the etherification tank, and a first pneumatic valve, a pressure gauge, a branch pipeline interface and a temperature and pressure regulating valve are sequentially arranged from the nitrogen storage tank to the etherification tank; one end of the second connecting pipeline is communicated with the ethylene oxide storage tank, and the other end of the second connecting pipeline is communicated with the branch pipeline interface; an automatic flow regulating valve is arranged on the second connecting pipeline close to the interface of the branch pipeline; the PLC is electrically connected with the first pneumatic valve, the pressure gauge, the temperature and pressure regulating valve, the metering flowmeter and the automatic flow regulating valve. According to the utility model, through the closed automatic gas transmission system, the accurate metering and safe use of ethylene oxide are realized, and the safety risk in the production process is reduced.

The invention relates to the technical field of epidemic disease detection of aquatic animals, in particular to a composite freeze-drying protective agent for a giant salamander iridovirus detection reagent and a preparation method of the reagent. The composite freeze-drying protective agent is mainly prepared from the following components: trehalose, glucan and hydroxyethyl starch according to the mass ratio of (3 to 5) to (0.8 to 1.2) to (0.3 to 0.7). The three-component composite protective agent is adopted, trehalose, dextran 40 and hydroxyethyl starch are mixed for use, protein is effectively protected, the glass transition temperature is prevented from being too high or too low, and redissolution and caking are inhibited. In the freeze-drying process of the detection reagent based on the protective agent, Q92R / E118K double mutation is performed on Taq enzyme, so that a dual protection mechanism for freeze-drying damage is formed; stepwise heating is introduced in the sublimation stage, and the freeze-drying time is shortened to 12 h; the detection reagent is treated through the gradient annealing freeze-drying process, so that the enzyme retention rate can reach 95% or above. The zero turbidity (OD600 is less than 0.05) of the redissolved reagent is ensured, and the interference of fluorescence detection is eliminated.

The invention discloses a preparation method of concentrated red blood cells from umbilical cord blood, and belongs to the field of biomedical engineering.According to the technical scheme, the preparation method comprises the following steps that umbilical cord blood is collected, whole umbilical cord blood is directly connected with a triple bag and then subjected to 600 * g centrifugation for 7 min at the temperature of 4 DEG C, umbilical cord blood plasma, umbilical cord blood mononuclear cells and suspended red blood cells are separated out, and the umbilical cord blood mononuclear cells and the suspended red blood cells are collected; and continuously centrifuging the obtained suspension red blood cells at 4 DEG C for 7min by 5000 * g, further concentrating the red blood cells, and removing upper plasma to obtain the concentrated red blood cells of the umbilical cord blood. According to the invention, 6% hydroxyethyl starch which is conventionally used is not added during preparation, but after technical optimization, aggregation and precipitation of red blood cells due to structural change are avoided, and the oxygen storage and transportation functions of the red blood cells are reserved, so that the red blood cells can be used as a red blood cell resource source. The hematocrit of the concentrated red blood cells obtained by the method is higher than 80%, the hemoglobin content recovery rate is higher than 55%, and the concentrated red blood cells can be used for autologous retransfusion of red blood cells during anemia of neonates, especially premature infants.

The invention discloses a manufacturing method of Tibetan paper for student exercise books, and relates to the field of Tibetan paper. Comprising the following steps: pretreatment of raw materials: stellera chamaejasme bast is soaked in plant ash liquid to be cooked, and then ozone bleaching is adopted; composite pulping: respectively pulping stellera chamaejasme bast fibers, bamboo pulp fibers and waste workbook recycled fibers, and then mixing; adding an auxiliary agent, namely adding the auxiliary agent into the mixed slurry; papermaking, namely diluting the pulp, forming on a net, dehydrating, squeezing, carrying out gradient drying, and finally, carrying out surface sizing; and post-treatment: carrying out calendaring treatment and slitting treatment on the glued raw materials. According to the method, the whiteness is improved through ozone bleaching, the ink absorption speed is increased through 5% hydroxyethyl starch surface sizing, meanwhile, gradient drying and calendaring are adopted, the wear resistance is improved, in addition, constant-pressure squeezing control is adopted, the thickness deviation is reduced, meanwhile, recycled fibers are adopted for replacement, and the cost is reduced.

The invention discloses a method for detecting the content of protein in hydroxyethyl starch by adopting a biuret reagent, which comprises the following steps of: 1, preparing reference substance solutions: carrying out biuret reagent reaction on different concentrations of protein content RS2 national standard substances to obtain different concentrations of reference substance solutions with ultraviolet absorption; 2, calculating a linear regression equation, measuring absorbance at the wavelength of 540nm, and calculating the linear regression equation according to the concentration of the reference substance solution and the absorbance corresponding to the reference substance solution; 3, preparing a test sample solution, and carrying out biuret reagent reaction on hydroxyethyl starch to be detected to obtain an ultraviolet absorption test sample solution; and 4, calculating the protein content in the sample solution to be tested according to the linear regression equation, measuring absorbance at the wavelength of 540nm, calculating the protein concentration in the sample solution to be tested according to the linear regression equation, multiplying by the dilution multiple, and dividing by the amount of the hydroxyethyl starch sample, so as to obtain the content of the protein in the sample solution to be tested. The detection method is safe and reliable.

The invention discloses blood substitute based on bletilla striata polysaccharide and a preparation method thereof, and relates to the technical field of blood substitute. The blood substitute is prepared from the following components in parts by weight: 3 to 5 parts of hydroxyethyl starch, 0.1 to 0.5 part of bletilla striata polysaccharide, 0.1 to 0.5 part of dendrobium officinale polysaccharide, 0.1 to 0.5 part of pullulan, 0.1 to 1 part of sodium chloride, 0.01 to 0.05 part of potassium chloride, 0.01 to 0.05 part of calcium chloride dihydrate, 0.01 to 0.05 part of magnesium chloride hexahydrate, 0.1 to 0.5 part of sodium lactate, 0.05 to 0.1 part of sodium acetate and 100 parts of water for injection. The plasma substitute provided by the invention has an excellent capacity expansion effect.

The invention discloses a degradable polyurethane film and a preparation method thereof. The polyurethane film is prepared from the following components in parts by mass: 7.5 to 11 parts of polyurethane, 78 to 85 parts of water, 2.4 to 4.5 parts of hydroxyethyl starch, 1.3 to 2.5 parts of methyl cellulose, 16 to 3.2 parts of acetyl tributyl citrate, 0.85 to 1.3 parts of plasticizer, 0.1 to 0.16 part of cross-linking agent and 0.5 to 1.6 parts of reinforcing agent. According to the invention, polyurethane is used as a matrix, hydroxyethyl starch and methyl cellulose are cooperatively modified, repeated freezing and thawing are carried out, and a coating film-forming process is adopted to prepare the coating film. By regulating and controlling the compounding ratio and the cross-linked network structure of the material, the balance of high mechanical strength (the tensile strength is greater than or equal to 10MPa) and rapid degradation performance (the weight loss ratio is greater than 90% within 60 days in a natural environment) is realized, and the functional expansion in environmental monitoring is realized. Compared with a traditional PE film, the product has the advantages of degradability, barrier property and cost, is suitable for the fields of medical packaging and the like, and has remarkable environmental protection value and industrialization prospect.

The present invention discloses a method for preparing lyophilized exosome powder, which belongs to the technical field of preparation of lyophilized exosome powder. The lyophilization protectant in the present invention includes an active component and a solution for dissolving the active component, wherein the active component includes trehalose, polyvinyl pyrrolidone, compound amino acids, taurine and hydroxyethyl starch, and the solution is a PBS solution. When preparing lyophilized exosome powder, the thawed exosome stock solution is mixed with the lyophilization protectant, and then the mixture is placed in a freeze dryer and freeze-dried under a set freezing program. The use of the cryoprotectant and freeze-drying method in this application can effectively protect the exosomes from external damage during the freeze-drying process, and can effectively ensure the biological activity of the exosomes.

The application belongs to the technical field of biological medicine, and particularly relates to a new indocyanine green delivery system targeting mitochondria of tumor cells and a preparation method thereof. The new indocyanine green and diamine are used to prepare aminylated new indocyanine green through a substitution reaction, and then the aminylated new indocyanine green is condensed with 3-propylcarboxyl triphenyl phosphonium bromide to prepare a triphenyl phosphonium salt-new indocyanine green conjugate; hydroxyethyl starch and perfluorooctadecanoic acid are used to prepare perfluorooctadecanoic acid hydroxyethyl starch ester through a condensation reaction, and then the perfluorooctadecanoic acid hydroxyethyl starch ester and the triphenyl phosphonium salt-new indocyanine green conjugate are dissolved in dimethyl sulfoxide to prepare the new indocyanine green delivery system targeting mitochondria of tumor cells. The delivery system has the effect of slow release of the triphenyl phosphonium salt-new indocyanine green conjugate, and has a stronger in-vitro photodynamic effect and a stronger photodynamic killing effect on tumor cells than the triphenyl phosphonium salt-new indocyanine green conjugate.

The present invention belongs to the field of modern bio-agriculture, and particularly relates to the application of alginate oligosaccharide as a nutritional regulator in crops. The nutritional regulator is composed of the following active ingredients in parts by weight: 10 parts of alginate oligosaccharide, 2-4 parts of ellagic acid, 4-8 parts of sodium hyaluronate, 5-10 parts of catechin, 5-10 parts of tocopheryl acetate, 5-10 parts of compound amino acid, and 10-20 parts of hydroxyethyl starch. The crops are woody vine plants. The present invention also discloses a preparation method and a specific usage method of the above nutritional regulator. The nutritional regulator of the present invention has low production cost, is convenient for transportation and storage, has no chemical residue, and can effectively promote the yield and disease resistance of woody vine plant economic crops, especially grapes and kiwifruits.

The invention discloses a preparation method of a biogenic vein containing a venous valve, and belongs to the technical field of biomedical functions.The preparation method of the biogenic vein containing the venous valve comprises the following steps that firstly, a pig or cattle jugular vein pipeline containing a complete valve is cut, cleaned, disinfected and flushed for use; sequentially carrying out cell lysis, cell digestion and nucleic acid digestion treatment; immersing into a 1 mol / L hydroxylamine sulfate solution to carry out heparin covalent cross-linking; immersing into a treating fluid to obtain a biogenic vein containing a venous valve; wherein the treating fluid is a PBS (Phosphate Buffer Solution) containing 4wt% of hydroxyethyl starch and 0.1 wt% of polyhexamethylene biguanide. According to the preparation method of the biogenic vein containing the venous valve, provided by the invention, the treatment liquid containing 4wt% of hydroxyethyl starch and 0.1 wt% of polyhexamethylene biguanide is introduced in the preparation process, so that the technical problem of insufficient dehydration and bacteriostasis in the background technology is remarkably solved, and meanwhile, the valve functionality of the biogenic vein is remarkably improved.

The invention discloses a disperse wet sizing digital printing paste composition for a Single Pass digital printing machine. The disperse wet sizing digital printing paste composition comprises hydroxyethyl starch, a dispersing agent, a gellan gum thickening agent, an ethyl hydroxyethyl cellulose thickening agent, a humectant, a pH regulator, a defoaming agent and a preservative. A preparation method of a disperse wet sizing digital printing paste composition for a Single Pass digital printing machine comprises the following steps: sequentially adding hydroxyethyl starch, a dispersing agent, a gellan gum thickening agent, an ethyl hydroxyethyl cellulose thickening agent, a humectant, a pH regulator, a defoaming agent and a preservative into a stirring kettle, and uniformly stirring under the stirring condition at the speed of 350-550r / min to obtain the disperse wet sizing digital printing paste composition for the Single Pass digital printing machine. The stirring time is 40-50 minutes, so as to obtain the final digital printing paste composition. The sizing agent has excellent ink locking capacity, efficient sizing effect and strong fabric adhesive force, has remarkable advantages in the aspects of color yield and definition, can effectively improve the digital printing quality, and meets the requirements of the textile printing and dyeing industry for sizing of high-quality digital printing fabric.

The invention relates to the technical field of cell freezing medium. The invention provides a mesenchymal stem cell cryopreservation solution and a cell cryopreservation and recovery method. The mesenchymal stem cell cryopreservation solution comprises a liquid component and a solid component, the liquid component comprises water, DMSO (dimethylsulfoxide), ethylene glycol, glycerol and human serum albumin injection; the solid component is prepared from mannose, hydroxyethyl starch, acetylchitosamine, trehalose and bilobalide. According to the cell cryopreservation liquid, by improving the formula, the dosage of DMSO is effectively reduced, the toxicity of DMSO is remarkably reduced, animal source components are avoided through the serum-free design, and the requirement for clinical safety is met. Meanwhile, specific programmed cooling equipment is not needed, the using method is simple and convenient, preservation is convenient, and the recovery survival rate after cryopreservation is larger than or equal to 90%.

The invention belongs to the technical field of biological medicines, and particularly relates to an amniotic mesenchymal stem cell injection and a large-scale construction method thereof. The amniotic mesenchymal stem cell injection is prepared from amniotic mesenchymal stem cells, a compound electrolyte injection, hydroxyethyl starch, low-molecular-weight heparin calcium, vitamin C, trehalose, ophiopogonic acid, ganoderma capense glycopeptide and water. The amniotic mesenchymal stem cell injection provided by the invention can effectively maintain the cell viability and osteogenic differentiation capacity of amniotic mesenchymal stem cells, and additives are clinically common drugs, so that the amniotic mesenchymal stem cell injection is safe and has no side effects.

The invention relates to the technical field of cryopreservation liquid, and discloses a serum-free cryopreservation liquid preparation method, which comprises: sucking 1-20 parts by volume of dimethyl sulfoxide, weighing 1-5 parts by volume of hydroxyethyl starch, weighing 1-5 parts by volume of sucrose, using a phosphate buffer solution as a solvent, mixing, filtering to remove bacteria, and storing to obtain the serum-free cryopreservation liquid, the serum-free cryopreservation liquid is easy for experimental analysis of influence factors of cells, does not contain animal serum and other heterologous proteins, also reduces the possibility that the cells are polluted, keeps the biological characteristics and survival rate of the cells, effectively ensures the differentiation capacity of the cells, is simple to operate and good in cryopreservation effect, can be directly used as an auxiliary material, and has a wide application prospect. The device has the characteristics of convenience in use and low cost.