15 results about "Hydroxyethyl starch" patented technology

The invention relates to the technical field of freeze-drying preparations, in particular to a plasma protective agent, a freeze-drying method for keeping the pH of plasma and freeze-dried plasma, and the plasma protective agent comprises the following components: an instant protective agent which comprises 5mg / ml of glycine; the feed additive is prepared from sodium dihydrogen phosphate, trehalose, hydroxyethyl starch, polyethylene glycol, mannitol, taurine, tryptophan lysine or glutamic acid. The sustained-release protective agent comprises antioxidant components encapsulated by lipidosome, wherein the antioxidant components comprise glutathione, adenosine and vitamin C. The invention also discloses a preparation method of the sustained-release protective agent. The novel protective agent vitamin C is adopted, the plasma pH adjusting operation is remarkably simplified, and the vitamin C is composed of multiple components with different functions, so that the stability of key components such as blood coagulation factors in plasma is greatly improved.

The invention discloses a drying protective agent for nuclear signal cell climbing slide. The drying protective agent comprises the following components: 80 g / L of trehalose, 30 g / L of sucrose, 5% of mannitol by volume fraction, 0.5% of TritonX-100 by volume fraction, 30 g / L of hydroxyethyl starch, 40 g / L of BSA, 40 g / L of sodium carboxymethyl cellulose, 40 g / L of polyvinylpyrrolidone, 10 g / L of cysteine and 5-7 g / L of sodium citrate. In the protective agent, TritonX-100 can pass through a cell membrane to release nucleoprotein, sample or antibody incubation can be directly carried out without permeation in subsequent detection, and other effective components can be loaded into cells to play a good stabilizing and protecting role in the cell drying process, so that morphological variation and damage of the cells are effectively avoided, antigen protein conformation is maintained, and the cell quality is improved. Therefore, the stability of the dried cells is improved.

The invention relates to a modified hydroxyethyl starch. According to the invention, the hydroxyethyl starch carries a heptonic acid residue on at least one of its termini. The invention further relates to a method for preparation such a starch and to novel uses of such a starch.

The invention discloses a method for detecting the content of protein in hydroxyethyl starch by adopting a biuret reagent, which comprises the following steps of: 1, preparing reference substance solutions: carrying out biuret reagent reaction on different concentrations of protein content RS2 national standard substances to obtain different concentrations of reference substance solutions with ultraviolet absorption; 2, calculating a linear regression equation, measuring absorbance at the wavelength of 540nm, and calculating the linear regression equation according to the concentration of the reference substance solution and the absorbance corresponding to the reference substance solution; 3, preparing a test sample solution, and carrying out biuret reagent reaction on hydroxyethyl starch to be detected to obtain an ultraviolet absorption test sample solution; and 4, calculating the protein content in the sample solution to be tested according to the linear regression equation, measuring absorbance at the wavelength of 540nm, calculating the protein concentration in the sample solution to be tested according to the linear regression equation, multiplying by the dilution multiple, and dividing by the amount of the hydroxyethyl starch sample, so as to obtain the content of the protein in the sample solution to be tested. The detection method is safe and reliable.

The invention discloses blood substitute based on bletilla striata polysaccharide and a preparation method thereof, and relates to the technical field of blood substitute. The blood substitute is prepared from the following components in parts by weight: 3 to 5 parts of hydroxyethyl starch, 0.1 to 0.5 part of bletilla striata polysaccharide, 0.1 to 0.5 part of dendrobium officinale polysaccharide, 0.1 to 0.5 part of pullulan, 0.1 to 1 part of sodium chloride, 0.01 to 0.05 part of potassium chloride, 0.01 to 0.05 part of calcium chloride dihydrate, 0.01 to 0.05 part of magnesium chloride hexahydrate, 0.1 to 0.5 part of sodium lactate, 0.05 to 0.1 part of sodium acetate and 100 parts of water for injection. The plasma substitute provided by the invention has an excellent capacity expansion effect.

The application belongs to the technical field of biological medicine, and particularly relates to a new indocyanine green delivery system targeting mitochondria of tumor cells and a preparation method thereof. The new indocyanine green and diamine are used to prepare aminylated new indocyanine green through a substitution reaction, and then the aminylated new indocyanine green is condensed with 3-propylcarboxyl triphenyl phosphonium bromide to prepare a triphenyl phosphonium salt-new indocyanine green conjugate; hydroxyethyl starch and perfluorooctadecanoic acid are used to prepare perfluorooctadecanoic acid hydroxyethyl starch ester through a condensation reaction, and then the perfluorooctadecanoic acid hydroxyethyl starch ester and the triphenyl phosphonium salt-new indocyanine green conjugate are dissolved in dimethyl sulfoxide to prepare the new indocyanine green delivery system targeting mitochondria of tumor cells. The delivery system has the effect of slow release of the triphenyl phosphonium salt-new indocyanine green conjugate, and has a stronger in-vitro photodynamic effect and a stronger photodynamic killing effect on tumor cells than the triphenyl phosphonium salt-new indocyanine green conjugate.

The invention relates to the technical field of cryopreservation liquid, and discloses a serum-free cryopreservation liquid preparation method, which comprises: sucking 1-20 parts by volume of dimethyl sulfoxide, weighing 1-5 parts by volume of hydroxyethyl starch, weighing 1-5 parts by volume of sucrose, using a phosphate buffer solution as a solvent, mixing, filtering to remove bacteria, and storing to obtain the serum-free cryopreservation liquid, the serum-free cryopreservation liquid is easy for experimental analysis of influence factors of cells, does not contain animal serum and other heterologous proteins, also reduces the possibility that the cells are polluted, keeps the biological characteristics and survival rate of the cells, effectively ensures the differentiation capacity of the cells, is simple to operate and good in cryopreservation effect, can be directly used as an auxiliary material, and has a wide application prospect. The device has the characteristics of convenience in use and low cost.

The invention provides a rapid replacement cell imaging buffer solution, which is prepared by optimizing components such as hydroxyethyl starch, C12-14 fatty alcohol etherified derivatives, polyvinyl alcohol, sodium citrate, polyaspartic acid, water-soluble vitamin E and the like as well as addition proportions. The multifunctional cell imaging rapid replacement buffer solution with good adsorption resistance, suspension dispersion, photobleaching resistance and decontamination cleaning is provided, sample residues can be rapidly and efficiently cleaned and detected, and high-throughput rapid and accurate detection of cell samples is promoted.

The application belongs to the technical field of biological medicine preparation, and particularly relates to a chlorin e6-loaded nanocluster activated by X-rays and a preparation method thereof. Hydroxyethyl starch and succinic anhydride are reacted in the presence of N,N-dimethylaminopyridine to obtain carboxylated hydroxyethyl starch, zinc oxide nanoparticles are assembled on the carboxylated hydroxyethyl starch to form zinc oxide nanoclusters, and then chlorin e6 is assembled on the zinc oxide nanoclusters. The obtained zinc oxide nanocluster loaded with chlorin e6 has a cluster structure, can generate singlet oxygen under X-ray irradiation, and can play an X-ray-induced photodynamic therapy role, and has potential application value in the photodynamic therapy of deep tumors.

The present application relates to a kind of high drug loading density somatostatin oral nanoparticles, the nanoparticles are spherical hydrophobic polyester material as carrier, several hydrophobic somatostatin ion pair complex particles are uniformly wrapped inside, and hydrophilic hydroxyethyl starch is grafted on the outer surface of polyester material, wherein the hydrophobic somatostatin ion pair complex is composed of somatostatin and counterion compound, and the counterion compound is at least one of sodium dodecyl sulfate, 8- (2-hydroxybenzamide) sodium octanoate, sodium oleate, sodium octanoate, sodium caprate, sodium laurate, sodium dodecyl sulfonate, sodium dioctyl sulfosuccinate;The hydrophobic polyester material is lactic acid-glycolic acid copolymer, polycaprolactone, polylactic acid.The nanoparticles are modified by hydroxyethyl starch, can quickly penetrate mucus barrier during oral delivery, and have high enterocyte penetration efficiency, can effectively improve the oral bioavailability of somatostatin.

Described herein are DMSO formulations and methods for cell therapy. Methods of treating a cell with these formulations are also provided. The embodiments provide a formulation that maintains the suspension and viability of a T-cell during cell therapy (CT) manufacturing and through a cryopreservation thaw cycle, including dimethyl sulfoxide (DMSO); sucrose or trehalose; human semm albumin (HSA); and dextran 40 or hydroxyethyl starch (HES). wherein the formulation maintains the suspension and viability of T-cells during CT manufacturing and through a cryopreservation thaw cycle. The embodiments provide a method for maintaining a suspension and cell viability, including subjecting a T-cell during a cell therapy manufacturing cryopreservation thaw cycle to a formulation including DMSO; sucrose or trehalose; human semm albumin (HSA); and polysaccharide or HES wherein the formulation maintains the suspension and viability of a T-cell during cell therapy manufacturing and through a cryopreservation thaw cycle.

Cryopreservation of endothelial cell monolayers is one of the major challenges in the cryopreservation of complex tissues. Human umbilical vein endothelial cells (HUVECs) in suspension are available commercially and recently their post-thaw cell membrane integrity was significantly improved by cryopreservation in 5% dimethyl sulfoxide (DMSO) and 6% hydroxyethyl starch (HES). However, cryopreservation of cells in monolayers has been elusive. The exact mechanisms of damage during cell monolayer cryopreservation are still under investigation. Here, we show that a combination of different factors contribute to significant progress in cryopreservation of cell monolayers. The addition of 2% chondroitin sulfate to 5% DMSO and 6% HES and cooling at 0.2 or 1° C. / min led to high membrane integrity (97.3±3.2%) immediately after thaw when HUVECs were cultured on a substrate with a coefficient of thermal expansion similar to that of ice. The optimized cryopreservation protocol was applied to monolayers of primary porcine corneal endothelial cells, and resulted in high post-thaw viability (95.9±3.7% membrane integrity) with metabolic activity 12 hours post-thaw comparable to unfrozen control.

The application relates to a skin model, in particular to a serum-free 3D skin model culture medium and application; a serum-free in-vitro 3D skin model culture medium of a system, which comprises an under-liquid culture solution, a gas-liquid surface culture solution and a freezing protective solution; the freezing protective solution takes MCDB153 culture solution as a basic culture medium, and HEPES, sodium bicarbonate, L-glutamine, a bacteriostatic agent, propylene glycol, sucrose, polyvinylpyrrolidone, hydroxyethyl starch, reduced glutathione, Y-27632 and vitamin E are added, and the beneficial effect is that: through pure product compounding of clear components, natural complex components of serum are replaced, and component uncertainty and batch difference caused by animal source factors are eliminated; by virtue of the design of no animal source components, the risk of carrying exogenous pollutants by serum is completely avoided.

The invention discloses an organoid cryopreservation solution, an organoid cryopreservation method, a kit and application thereof, and relates to the technical field of organoid cryopreservation. The invention relates to a D-trehalose freeze-dried powder which only contains the following raw materials: 8%-12% (w / w) of D-trehalose, 10% (w / w) of hydroxyethyl starch, 10% (w / w) of BSA (Bovine Serum Albumin), 10-12 [mu] M of Y27632 and 4%-10% of DMSO (Dimethylsulfoxide) in percentage by volume. The organoid cryopreservation liquid provided by the invention can effectively retain the biological characteristics of the organoid before cryopreservation and retain the original activity of the organoid.

The application discloses a kind of cell spheroid serum-free cryopreservation solution and its preparation method and application, steps are mixed to complete mixing with a certain mass of DMSO, ethylene glycol and glycerol, as composite osmotic protection phase;A certain mass of methyl cellulose, trehalose and hydroxyethyl starch are added in a certain volume of ultrapure water and stirred to dissolve, then a certain mass of cholesterol, Y27632 and glutathione are stirred to completely dissolve, as non-osmotic protection phase;A certain mass of composite osmotic protection phase and non-osmotic protection phase are mixed, and a certain mass of KOSR serum substitute, L-alanyl-L-glutamine and insulin is added, slowly stirred and filtered to remove bacteria, to obtain cell spheroid serum-free cryopreservation solution.The cell spheroid cryopreservation solution prepared by the application combines low-toxicity composite osmotic protection and multiple non-osmotic protection, realizes high recovery survival rate and stable structure maintenance, and the process is simple, which is very suitable for the cryopreservation of multiple types of cell spheroids.