33 results about "Hydroxyethyl starch" patented technology

The invention relates to the technical field of organ transplantation, and discloses organ preserving fluid, application and organ dynamic supercooling perfusion equipment. The organ preserving fluid is prepared from ribose, glucose, mannitol, lidocaine, adenosine, a high-molecular polymer, L-proline, trehalose, inorganic salt and organic salt. The high-molecular polymer comprises one of hydroxyethyl starch, polyethylene glycol and albumin. According to the organ preserving fluid disclosed by the invention, the freezing point of the perfusate is reduced through the micromolecular cryoprotectant, and meanwhile, the micromolecular cryoprotectant and the high-molecular polymer jointly inhibit formation and growth of ice crystals; the problem of liquid preservation at the temperature below zero is solved from the two dimensions of physics (reducing the freezing point and inhibiting ice crystals) and biology (maintaining cell viability), and feasibility is provided for long-time low-temperature transfer and transplantation of organs. The preserving fluid can be used for static preservation or perfusion preservation of various organs such as kidney, liver, heart, pancreas, lung, small intestine, skin and the like.

The invention discloses a method for calculating the molar substitution degree of hydroxyethyl starch by applying a nuclear magnetic resonance carbon spectrum, and belongs to the technical field of analysis and detection. The method comprises the following steps: carrying out nuclear magnetic resonance test on a sample to be detected to obtain a nuclear magnetic resonance 13C spectrum, and determining the molar substitution degree of hydroxyethyl starch according to the peak integral ratio of hydroxyethyl alpha carbon or beta carbon in hydroxyethyl starch glucose to glucose 1-position heterogenous carbon; compared with a traditional Morgan method and a traditional gas chromatography method, the method has the advantages that the operation process is simplified, time and labor are saved, the consumption of experimental consumables and reagents is greatly reduced, the molar substitution degree can be intuitively calculated on the basis of not damaging the structure of a sample, and the method is simpler, more convenient, high in accuracy, simple and convenient to operate and high in detection speed; the method can be used for detecting the molar substitution degree of hydroxyethyl starch.

The invention relates to the technical field of freeze-drying preparations, in particular to a plasma protective agent, a freeze-drying method for keeping the pH of plasma and freeze-dried plasma, and the plasma protective agent comprises the following components: an instant protective agent which comprises 5mg / ml of glycine; the feed additive is prepared from sodium dihydrogen phosphate, trehalose, hydroxyethyl starch, polyethylene glycol, mannitol, taurine, tryptophan lysine or glutamic acid. The sustained-release protective agent comprises antioxidant components encapsulated by lipidosome, wherein the antioxidant components comprise glutathione, adenosine and vitamin C. The invention also discloses a preparation method of the sustained-release protective agent. The novel protective agent vitamin C is adopted, the plasma pH adjusting operation is remarkably simplified, and the vitamin C is composed of multiple components with different functions, so that the stability of key components such as blood coagulation factors in plasma is greatly improved.

The invention provides a bovine embryo refrigerated preservation solution. The bovine embryo refrigerated preservation solution is prepared from hydroxyethyl starch, dimethyl sulfoxide, fetal calf serum, cane sugar, cepharanthine and deionized water. The ratio of the components is further optimized, and the components comprise 5% of hydroxyethyl starch, 5% of dimethyl sulfoxide, 55% of fetal calf serum, 37% of deionized water, 2% of cane sugar and 1% of cepharanthine. Under the optimal raw material ratio condition that the concentration of cepharanthine is 600 [mu] mol / L and the concentration of cane sugar is 2 mol / L, cepharanthine is added into the bovine embryo preserving fluid, through a triple synergistic mechanism of oxidation resistance, apoptosis resistance and immunoregulation, the defect of biochemical damage protection in the prior art is overcome, and full-period protection is provided for embryos.

The invention discloses a drying protective agent for nuclear signal cell climbing slide. The drying protective agent comprises the following components: 80 g / L of trehalose, 30 g / L of sucrose, 5% of mannitol by volume fraction, 0.5% of TritonX-100 by volume fraction, 30 g / L of hydroxyethyl starch, 40 g / L of BSA, 40 g / L of sodium carboxymethyl cellulose, 40 g / L of polyvinylpyrrolidone, 10 g / L of cysteine and 5-7 g / L of sodium citrate. In the protective agent, TritonX-100 can pass through a cell membrane to release nucleoprotein, sample or antibody incubation can be directly carried out without permeation in subsequent detection, and other effective components can be loaded into cells to play a good stabilizing and protecting role in the cell drying process, so that morphological variation and damage of the cells are effectively avoided, antigen protein conformation is maintained, and the cell quality is improved. Therefore, the stability of the dried cells is improved.

The invention belongs to the technical field of biological medicine, and particularly relates to a normal-temperature cell protective agent, a normal-temperature cell protective solution and application thereof. The normal-temperature cell protective agent is prepared from human serum albumin, trehalose, 6-amino nicotinamide, hydroxyethyl starch and compound electrolyte. The normal-temperature cell preserving fluid comprises the normal-temperature cell protecting agent and a physiologically acceptable carrier. According to the normal-temperature cell preservation solution, normal-temperature transportation of stem cells can be achieved, the motility rate of the stem cells is not affected by external temperature change in a short time (within 3 days), the cells are well protected within the temperature range of 4-25 DEG C, the cell survival rate is high, the cell survival rate can reach 97% or above within 72 h, and the normal-temperature cell preservation solution is remarkably superior to an existing cell preservation solution.

The invention relates to a modified hydroxyethyl starch. According to the invention, the hydroxyethyl starch carries a heptonic acid residue on at least one of its termini. The invention further relates to a method for preparation such a starch and to novel uses of such a starch.

The invention discloses a hepatocyte cryopreservation solution which comprises a first cryopreservation mixed solution, a second cryopreservation mixed solution and fetal calf serum, the first cryopreservation mixed solution is prepared from a Leibovitz's L-15 culture medium, lactobionic acid, raffinose, reduced glutathione, ademetionine, curcumin, bicyclol, ursodesoxycholic acid, silymarin and glycyrrhizic acid; the second cryopreservation mixed solution comprises cane sugar, trehalose, hydroxyethyl starch and polyene phosphatidylcholine. On the other hand, the invention discloses a cryopreservation method and a recovery method of primary mouse hepatocytes based on the hepatocyte cryopreservation liquid. The cryopreservation method comprises the following steps: pre-culturing, re-suspending, slowly dropwise adding DMSO (dimethylsulfoxide) and cooling and cryopreserving step by step. The resuscitation method comprises the steps of rapid unfreezing, centrifugal purification and resuscitation culture. The liver cell cryopreservation liquid and the cryopreservation and resuscitation method can play roles in regulating osmotic pressure, inhibiting ice crystal formation, stabilizing cell membrane structure, resisting oxidation, promoting protein synthesis and the like, and the resuscitated cells are high in survival rate and strong in adherence capability and functional activity.

The invention discloses a method for detecting the content of protein in hydroxyethyl starch by adopting a biuret reagent, which comprises the following steps of: 1, preparing reference substance solutions: carrying out biuret reagent reaction on different concentrations of protein content RS2 national standard substances to obtain different concentrations of reference substance solutions with ultraviolet absorption; 2, calculating a linear regression equation, measuring absorbance at the wavelength of 540nm, and calculating the linear regression equation according to the concentration of the reference substance solution and the absorbance corresponding to the reference substance solution; 3, preparing a test sample solution, and carrying out biuret reagent reaction on hydroxyethyl starch to be detected to obtain an ultraviolet absorption test sample solution; and 4, calculating the protein content in the sample solution to be tested according to the linear regression equation, measuring absorbance at the wavelength of 540nm, calculating the protein concentration in the sample solution to be tested according to the linear regression equation, multiplying by the dilution multiple, and dividing by the amount of the hydroxyethyl starch sample, so as to obtain the content of the protein in the sample solution to be tested. The detection method is safe and reliable.

The invention discloses blood substitute based on bletilla striata polysaccharide and a preparation method thereof, and relates to the technical field of blood substitute. The blood substitute is prepared from the following components in parts by weight: 3 to 5 parts of hydroxyethyl starch, 0.1 to 0.5 part of bletilla striata polysaccharide, 0.1 to 0.5 part of dendrobium officinale polysaccharide, 0.1 to 0.5 part of pullulan, 0.1 to 1 part of sodium chloride, 0.01 to 0.05 part of potassium chloride, 0.01 to 0.05 part of calcium chloride dihydrate, 0.01 to 0.05 part of magnesium chloride hexahydrate, 0.1 to 0.5 part of sodium lactate, 0.05 to 0.1 part of sodium acetate and 100 parts of water for injection. The plasma substitute provided by the invention has an excellent capacity expansion effect.

The application belongs to the technical field of biological medicine, and particularly relates to a new indocyanine green delivery system targeting mitochondria of tumor cells and a preparation method thereof. The new indocyanine green and diamine are used to prepare aminylated new indocyanine green through a substitution reaction, and then the aminylated new indocyanine green is condensed with 3-propylcarboxyl triphenyl phosphonium bromide to prepare a triphenyl phosphonium salt-new indocyanine green conjugate; hydroxyethyl starch and perfluorooctadecanoic acid are used to prepare perfluorooctadecanoic acid hydroxyethyl starch ester through a condensation reaction, and then the perfluorooctadecanoic acid hydroxyethyl starch ester and the triphenyl phosphonium salt-new indocyanine green conjugate are dissolved in dimethyl sulfoxide to prepare the new indocyanine green delivery system targeting mitochondria of tumor cells. The delivery system has the effect of slow release of the triphenyl phosphonium salt-new indocyanine green conjugate, and has a stronger in-vitro photodynamic effect and a stronger photodynamic killing effect on tumor cells than the triphenyl phosphonium salt-new indocyanine green conjugate.

The invention relates to the technical field of cryopreservation liquid, and discloses a serum-free cryopreservation liquid preparation method, which comprises: sucking 1-20 parts by volume of dimethyl sulfoxide, weighing 1-5 parts by volume of hydroxyethyl starch, weighing 1-5 parts by volume of sucrose, using a phosphate buffer solution as a solvent, mixing, filtering to remove bacteria, and storing to obtain the serum-free cryopreservation liquid, the serum-free cryopreservation liquid is easy for experimental analysis of influence factors of cells, does not contain animal serum and other heterologous proteins, also reduces the possibility that the cells are polluted, keeps the biological characteristics and survival rate of the cells, effectively ensures the differentiation capacity of the cells, is simple to operate and good in cryopreservation effect, can be directly used as an auxiliary material, and has a wide application prospect. The device has the characteristics of convenience in use and low cost.

The invention provides a rapid replacement cell imaging buffer solution, which is prepared by optimizing components such as hydroxyethyl starch, C12-14 fatty alcohol etherified derivatives, polyvinyl alcohol, sodium citrate, polyaspartic acid, water-soluble vitamin E and the like as well as addition proportions. The multifunctional cell imaging rapid replacement buffer solution with good adsorption resistance, suspension dispersion, photobleaching resistance and decontamination cleaning is provided, sample residues can be rapidly and efficiently cleaned and detected, and high-throughput rapid and accurate detection of cell samples is promoted.

The application belongs to the technical field of biological medicine preparation, and particularly relates to a chlorin e6-loaded nanocluster activated by X-rays and a preparation method thereof. Hydroxyethyl starch and succinic anhydride are reacted in the presence of N,N-dimethylaminopyridine to obtain carboxylated hydroxyethyl starch, zinc oxide nanoparticles are assembled on the carboxylated hydroxyethyl starch to form zinc oxide nanoclusters, and then chlorin e6 is assembled on the zinc oxide nanoclusters. The obtained zinc oxide nanocluster loaded with chlorin e6 has a cluster structure, can generate singlet oxygen under X-ray irradiation, and can play an X-ray-induced photodynamic therapy role, and has potential application value in the photodynamic therapy of deep tumors.

Disclosed are a cell cryopreservation protection solution and a use thereof. The cell cryopreservation protection solution comprises the following components: polyethylene glycol, hydroxyethyl starch, human serum albumin, and trehalose. The molecular weight of polyethylene glycol is 200-600. In the cell cryopreservation protection solution, a safe and stable cryopreservation environment is provided for cells by means of the combined use of polyethylene glycol, hydroxyethyl starch, human serum albumin, and trehalose having proper molecular weights, and the damage of a cryoprotectant to the cells is reduced, so that the cells subjected to cryopreservation and resuscitation have high cell viability and stability. The cell cryopreservation protection solution is especially suitable for cryopreservation of mesenchymal stem cells. In addition, the cell cryopreservation protection solution provided by the present invention does not contain DMSO, glycerol, animal serum and other reagents that are limited in clinical application, and can be directly injected into patients without requiring the process of centrifugation and washing to obtain cells, thereby providing feasibility for the clinical application of mesenchymal stem cells.

The present application relates to a kind of high drug loading density somatostatin oral nanoparticles, the nanoparticles are spherical hydrophobic polyester material as carrier, several hydrophobic somatostatin ion pair complex particles are uniformly wrapped inside, and hydrophilic hydroxyethyl starch is grafted on the outer surface of polyester material, wherein the hydrophobic somatostatin ion pair complex is composed of somatostatin and counterion compound, and the counterion compound is at least one of sodium dodecyl sulfate, 8- (2-hydroxybenzamide) sodium octanoate, sodium oleate, sodium octanoate, sodium caprate, sodium laurate, sodium dodecyl sulfonate, sodium dioctyl sulfosuccinate;The hydrophobic polyester material is lactic acid-glycolic acid copolymer, polycaprolactone, polylactic acid.The nanoparticles are modified by hydroxyethyl starch, can quickly penetrate mucus barrier during oral delivery, and have high enterocyte penetration efficiency, can effectively improve the oral bioavailability of somatostatin.

Described herein are DMSO formulations and methods for cell therapy. Methods of treating a cell with these formulations are also provided. The embodiments provide a formulation that maintains the suspension and viability of a T-cell during cell therapy (CT) manufacturing and through a cryopreservation thaw cycle, including dimethyl sulfoxide (DMSO); sucrose or trehalose; human semm albumin (HSA); and dextran 40 or hydroxyethyl starch (HES). wherein the formulation maintains the suspension and viability of T-cells during CT manufacturing and through a cryopreservation thaw cycle. The embodiments provide a method for maintaining a suspension and cell viability, including subjecting a T-cell during a cell therapy manufacturing cryopreservation thaw cycle to a formulation including DMSO; sucrose or trehalose; human semm albumin (HSA); and polysaccharide or HES wherein the formulation maintains the suspension and viability of a T-cell during cell therapy manufacturing and through a cryopreservation thaw cycle.

Cryopreservation of endothelial cell monolayers is one of the major challenges in the cryopreservation of complex tissues. Human umbilical vein endothelial cells (HUVECs) in suspension are available commercially and recently their post-thaw cell membrane integrity was significantly improved by cryopreservation in 5% dimethyl sulfoxide (DMSO) and 6% hydroxyethyl starch (HES). However, cryopreservation of cells in monolayers has been elusive. The exact mechanisms of damage during cell monolayer cryopreservation are still under investigation. Here, we show that a combination of different factors contribute to significant progress in cryopreservation of cell monolayers. The addition of 2% chondroitin sulfate to 5% DMSO and 6% HES and cooling at 0.2 or 1° C. / min led to high membrane integrity (97.3±3.2%) immediately after thaw when HUVECs were cultured on a substrate with a coefficient of thermal expansion similar to that of ice. The optimized cryopreservation protocol was applied to monolayers of primary porcine corneal endothelial cells, and resulted in high post-thaw viability (95.9±3.7% membrane integrity) with metabolic activity 12 hours post-thaw comparable to unfrozen control.

The present invention provides a method for isolating and culturing umbilical cord blood-derived hematopoietic stem cells, the method comprising preparing hematopoietic stem cells using a hydroxyethyl starch (HES) precipitation method, wherein a sodium selenite and phosphodipeptide are contained in a hydroxyethyl starch solution used. The hematopoietic stem cells obtained by the method of the present invention have increased recovery rate and increased removal rate of red blood cells.

This invention discloses a composite talc paper filler, its preparation method, and its application, belonging to the field of papermaking technology. The invention involves dissolving polylactic acid and didecyl dimethyl ammonium chloride in a dichloromethane / acetone mixed solvent, adding talc and polyethylene glycol octadecyl ether, and dispersing them evenly to obtain an oil phase. The oil phase is then added dropwise to a polyvinyl alcohol / hydroxyethyl starch aqueous solution and homogenized to obtain an emulsion. The organic solvent is removed from the emulsion to obtain solidified microspheres, which are then centrifuged, washed, and dried to obtain modified talc. The prepared composite talc paper filler has a core-shell structure and possesses cationic group characteristics on its surface, which is beneficial for improving the retention of fillers and fine fibers. Furthermore, the use of this composite talc paper filler improves paper performance, such as smoothness, surface strength, whiteness, and opacity, and enhances paper sizing and ink absorption.

The present invention relates to a conjugate represented by the formula [D—L—Y—(CH2)n-0]m-P—T wherein T is a protein; P is a polymer selected from the group consisting of dextran, mannan, pullulan, hyaluronic acid, hydroxyethyl starch, chondroitin sulphate, heparin, heparin sulphate, polyalkylene glycol, Ficoll, polyvinyl alcohol, amylose, amylopectin, chitosan, cyclodextrin, pectin and carrageenan, or a derivative thereof; m is at least 1; n is in the range of 1 to 10; each Y is independently selected from the group consisting of S, NH and 1,2,3-triazolyl, wherein 1,2,3-triazolyl is optionally substituted; each L is independently absent or comprises a linker group covalently joining D and Y; and each D is a payload molecule.

The application relates to a skin model, in particular to a serum-free 3D skin model culture medium and application; a serum-free in-vitro 3D skin model culture medium of a system, which comprises an under-liquid culture solution, a gas-liquid surface culture solution and a freezing protective solution; the freezing protective solution takes MCDB153 culture solution as a basic culture medium, and HEPES, sodium bicarbonate, L-glutamine, a bacteriostatic agent, propylene glycol, sucrose, polyvinylpyrrolidone, hydroxyethyl starch, reduced glutathione, Y-27632 and vitamin E are added, and the beneficial effect is that: through pure product compounding of clear components, natural complex components of serum are replaced, and component uncertainty and batch difference caused by animal source factors are eliminated; by virtue of the design of no animal source components, the risk of carrying exogenous pollutants by serum is completely avoided.

The invention discloses an organoid cryopreservation solution, an organoid cryopreservation method, a kit and application thereof, and relates to the technical field of organoid cryopreservation. The invention relates to a D-trehalose freeze-dried powder which only contains the following raw materials: 8%-12% (w / w) of D-trehalose, 10% (w / w) of hydroxyethyl starch, 10% (w / w) of BSA (Bovine Serum Albumin), 10-12 [mu] M of Y27632 and 4%-10% of DMSO (Dimethylsulfoxide) in percentage by volume. The organoid cryopreservation liquid provided by the invention can effectively retain the biological characteristics of the organoid before cryopreservation and retain the original activity of the organoid.

The invention relates to a skin model, in particular to a serum-free 3D skin model culture medium and application. The invention discloses an in-vitro 3D skin model culture medium of a serum-free system. The in-vitro 3D skin model culture medium comprises an under-liquid culture solution, a gas-liquid surface culture solution and a freezing protection solution, the freezing protection liquid takes an MCDB153 culture solution as a basic culture medium, and HEPES, sodium bicarbonate, L-glutamine, a bacteriostatic agent, propylene glycol, cane sugar, polyvinylpyrrolidone, hydroxyethyl starch, reduced glutathione, Y-27632 and vitamin E. The freezing protection liquid has the beneficial effects that pure products with definite components are compounded to replace natural complex components of serum; component uncertainty and batch difference caused by animal source factors are eliminated; by means of the design of animal-source-free components, the risk that the serum carries exogenous pollutants is thoroughly avoided.

This invention discloses a method for preparing a biogenic vein containing venous valves, belonging to the field of biomedical functional technology. The method includes the following steps: cutting a porcine or bovine jugular vein containing intact valves, cleaning, disinfecting, and rinsing it for later use; sequentially performing cell lysis, cell digestion, and nucleic acid digestion; then immersing it in a 1 mol / L hydroxylamine sulfate solution for heparin covalent cross-linking; and finally immersing it in a treatment solution to obtain a biogenic vein containing venous valves. The treatment solution is a PBS solution containing 4 wt% hydroxyethyl starch and 0.1 wt% polyhexamethylene biguanide. The method for preparing a biogenic vein containing venous valves provided by this invention significantly solves the technical problems of insufficient dehydration and antibacterial activity mentioned in the background art by introducing a treatment solution containing 4 wt% hydroxyethyl starch and 0.1 wt% polyhexamethylene biguanide during the preparation process. Simultaneously, the valve function of the biogenic vein is significantly improved.

The invention relates to a serum-free, animal-source-component-free and DMSO-free cell freezing medium and a preparation method and application thereof, and relates to the technical field of cell and biological engineering, and the serum-free, animal-source-component-free and DMSO-free cell freezing medium comprises the following components (according to the concentration range): 10-50mM of lacturonic acid; 50 to 200 mM of raffinose; 2-8% (w / v) of hydroxyethyl starch; 1 to 5 mM of monopotassium phosphate; 0.5 to 2 mM of magnesium sulfate; 1 to 5 mM of glutathione; allopurinol: 0.1 to 1 mM; 0.5 to 2 mM of adenosine; insulin: 0.1 to 1 [mu] g / mL; 50 to 150 mM of trehalose; proline: 20 to 100 mM; 10-20% (v / v) of glycerol; and a pH buffer. The application does not contain serum, animal-derived substances and DMSO, and avoids the problems of toxicity, immunogenicity and ethics.

The invention relates to an immune cell serum-free cryopreservation solution and a preparation method thereof, and belongs to the technical field of cell biology and biological pharmacy. According to the cryopreservation liquid, DMSO and DPBS are mixed according to the volume ratio of 10: 90 to form a buffer solution, hydroxyethyl starch, heparin sodium, trehalose, glutamine, HEMA and taurine are added into the buffer solution, a serum-free cryopreservation formula with definite components and high stability is formed, and the preparation method comprises the five steps of accurate component weighing, buffer solution preparation, dissolution and uniform mixing, constant volume and pH adjustment and sterilization and filtration. Wherein the component concentration can be adjusted within a preset interval according to experimental requirements, the cryopreservation liquid is suitable for immune cell preservation experiments under different conditions, the cryopreservation liquid does not contain serum components, the heterologous protein pollution risk can be remarkably reduced, and the survival rate and the biological function retention rate of cryopreserved cells are increased. The cell cryopreservation device is suitable for medium and long term cryopreservation of various immune cells such as CAR-T cells and NK cells, and has good biological safety and clinical application prospects.

The invention relates to the technical field of biological agents, in particular to a menstrual blood preserving fluid, which is characterized in that components such as modified hydroxyethyl starch, glucose, human serum albumin and the like are compounded to serve as components of the cryopreservation fluid according to a menstrual blood source mesenchymal stem cell cryopreservation method; the preparation method comprises the following steps: firstly, carrying out amination treatment on hydroxyethyl starch by adopting bromopropylamine hydrobromic acid to obtain aminated hydroxyethyl starch, and sequentially reacting the aminated hydroxyethyl starch with activated heparin sodium and proline so as to sequentially introduce the heparin sodium and the proline to obtain modified hydroxyethyl starch; according to the present invention, the modified hydroxyethyl starch is added, such that the hydration ability and the water binding ability of the modified hydroxyethyl starch can be significantly enhanced, and the binding water is difficult to form the large and sharp ice crystal during the freezing process so as to physically inhibit the growth of the ice crystal and reduce the damage of the ice crystal generated by the cryopreservation liquid during the freezing process to the cell membrane of the cell;

The invention discloses a plasma substitute surface engineering bioprosthetic valve material and a preparation method thereof, the preparation method comprises the following steps: S1, obtaining a bioprosthetic valve material, and pretreating the bioprosthetic valve material; s2, activating the pretreated bioprosthetic valve material by adopting an amide condensing agent to obtain a carboxyl activated bioprosthetic valve material; s3, grafting the bioprosthetic valve material after the carboxyl is activated by adopting 2-aminoethyl methacrylate hydrochloride to obtain a bioprosthetic valve material grafted with a methacrylate group; and S4, under the action of an initiator, carrying out polymerization reaction on methacrylated hydroxyethyl starch and the bioprosthetic valve material grafted with the methacrylate group, and cleaning to obtain the plasma substitute surface-engineered bioprosthetic valve material. According to the invention, the defects of toxicity, calcification, immunoreaction and the like caused by glutaraldehyde crosslinking of the existing bioprosthetic valve can be overcome, and the defects of thrombus and immunoreaction caused by heterogeneous collagen matrix of the bioprosthetic valve material can be overcome.

The invention discloses a cryopreservation system for functional parenchymal hepatic cells and a cryopreservation elution method of the cryopreservation system, and belongs to the technical field of biological medicine, and the cryopreservation system comprises an equilibrium solution, a cryopreservation solution and an elution solution. The cryopreservation liquid comprises a cryoprotectant composition, an amino acid stabilizer and a buffer solution, and the cryoprotectant composition is at least two of dimethyl sulfoxide, propylene glycol, glycerol, polyvinylpyrrolidone and hydroxyethyl starch. The cryopreservation and elution method comprises the steps of balancing, cryopreservation and elution. According to the invention, by reducing the content of dimethyl sulfoxide or completely replacing dimethyl sulfoxide, the potential toxic damage to cells caused by higher content in the traditional cryopreservation liquid is reduced, and the cell survival rate is improved. And moreover, the use of animal-derived components is completely eliminated, the permeation injury and toxic injury possibly suffered by the cells in the cryopreservation and recovery processes are reduced, and the method has important significance in promoting the research and application of the functional parenchymal hepatic cells in the fields of drug screening, disease model construction, cell therapy and the like.