53 results about "Cell survival" patented technology

The invention discloses an alpha nuclide drug in-vitro cellular response prediction method and system based on multi-module mathematical modeling and a medium, and the method comprises the steps: constructing a basic parameter input module, a pharmacokinetic prediction module, a cell absorbed dose calculation module and a biological effect prediction module which are connected in sequence; a three-compartment dynamical model and a micro-dose learning point kernel convolution and DNA damage repair dynamical model are integrated, and full-chain and quantitative simulation from drug distribution, microscopic energy deposition to cell survival is achieved. The method overcomes the defects that an existing empirical model cannot accurately reflect the high LET characteristic of alpha particles, neglects the bystander effect and repair dynamics and the like, and the prediction precision is remarkably improved. According to the method, the curative effects of different drugs, cell lines and dosage schemes can be quickly simulated on a computer, the research and development cost and period are greatly reduced, and an efficient theoretical tool is provided for new drug screening and dosage optimization.

The invention discloses application of lycium barbarum polysaccharide in preparation of a medicine for inhibiting methylamphetamine-induced nerve injury, and relates to the technical field of biological medicines. It is found and proved that lycium barbarum polysaccharide can inhibit methylamphetamine-induced nerve injury, and the effect is remarkable. From the aspect of cell survival, the survival rate of the microglial cells pretreated by the lycium barbarum polysaccharide after METH stimulation is obviously improved. In the molecular mechanism, the lycium barbarum polysaccharide can effectively lower the protein expression level of the METH-induced pyroptosis marker, the content of inflammatory factors IL-1beta and IL-18 can be reduced, and the release of lactic dehydrogenase is reduced. The result shows that the lycium barbarum polysaccharide can alleviate inflammatory response by inhibiting NLRP3 / ASC pathway mediated microglial cell pyroptosis, thereby inhibiting METH-induced nerve injury. The invention provides a new direction for developing METH neurotoxicity intervention medicines.

The invention belongs to the field of biological medicine, and discloses a membrane binding type IL7 fusion protein, an engineered immune cell for expressing the membrane binding type IL7 fusion protein and application of the membrane binding type IL7 fusion protein. The fusion protein comprises an IL7 region and a transmembrane domain and can be expressed on a cell membrane, the tumor cell killing ability and the T cell survival ability of T cells expressing the fusion protein are both enhanced, and the aims of improving the tumor immune cell treatment effect and reducing the toxic and side effects are achieved. Particularly, the IL7 fusion protein anchors and expresses IL7 on the surface of a cell through transmembrane domains such as CD80 or PD-L1 and the like, so that (1) immune cells can be accurately regulated and controlled, the possibility that an excessive IL7 signal possibly causes autoimmune response or aggravates CRS is reduced, and the safety of the IL7 to immune cells such as T cells and the like is enhanced; (2) the half-life period of IL7 is prolonged, and the anti-tumor effect of adoptive immune cells is enhanced; and (3) the transmembrane fragment is linked with the IL7 through a hinge region of the flexible linker G4S, CD80 or PD-L1, so that the flexibility of the IL7 is enhanced, and the proliferation and killing functions of the IL7 on T cells are enhanced.

The invention discloses siRNA capable of knocking down Pr18a9 gene expression and application of the siRNA, and relates to the technical field of biological medicine. The invention provides a positive-sense strand sequence and an antisense strand sequence of the siRNA, and the siRNA is used for preparing a preparation for promoting Schwann cell survival. According to the invention, the Prl8a9 gene of SCs is knocked down by virtue of a small interfering RNA technology; by improving the proliferation and migration capabilities of the Schwann cells, the apoptosis rate of the Schwann cells is reduced, and the survival rate of the Schwann cells is further improved. Meanwhile, a transcriptome sequencing technology is applied, key genes and pathways of Schwann cells treated by siPr18a9 are deeply excavated, and a theoretical support is provided for research on a repair mechanism after peripheral nerve injury.

The present application relates to the technical field of genetic engineering, and specifically discloses a Chlamydomonas reinhardtii SnRK2.7 gene and application thereof in adaptation to osmotic stress and maintenance of cell survival. The present application first discloses SnRK2.7 the key biological function of the gene in maintaining Chlamydomonas cell homeostasis. By knocking out the gene through CRISPR / Cas9 gene editing technology and adopting an expression frame driven by an endogenous promoter for genetic back complementation, it is confirmed that SnRK2.7 the gene positively regulates cell osmotic stress tolerance and maintains cell long-term survival. In addition, the present application first discovers that the SnRK2.7 protein is specifically located in the Chlamydomonas expansion vesicle, which provides direct spatial evidence for the cell function. The above discovery not only expands the understanding of the osmotic stress signal transduction mechanism of Chlamydomonas, but also provides a new gene target and research tool for the improvement of microalgae stress adaptability, and has important basic research value and biological technology application potential.

The invention belongs to the technical field of biological medicine, and particularly relates to a gene-edited immune cell and application thereof. The invention provides a composition. The composition comprises a cytokine and a chemokine receptor, the amplification and / or survival ability of cells containing or expressing the composition is enhanced, and the composition has a synergistic effect in promoting the survival and / or proliferation of the cells.

Methods are provided for generating unlimited immune cells with increased lifespan and high proliferation rate by modifying them to express BCL6 and genes that promote cell survival. Further methods are provided for generating and using said unlimited immune cells to treat diseases such as cancer.

The present invention relates to a chimeric protein of IL-2 protein and IL-15 protein, a variant thereof, a fusion protein comprising the same, and / or a genetically engineered cell modified to express any one or more thereof. In the present invention, the binding to IL-2Rβ is weakened, the side effects caused by the excessive immune cell activity of IL-2 can be inhibited, and the rapid exhaustion of natural killer cells is prevented, thereby allowing the appropriate level of cell activity and cell survival ability to be maintained for a long time. Accordingly, the present invention can be used for modulating the immune system and treating immune-related diseases, such as cancer, infectious diseases, and autoimmune diseases.

The invention relates to the technical field of medical engineering, in particular to an NK cell delivery data processing method and system based on magnetic targeting. The method comprises the following steps: inquiring an immune receptor protein structure of natural killer cells; injecting a sodium chloride solution, adding an immune receptor protein structure, and reacting to generate an aptamer structure; the method comprises the following steps: combining streptavidin in a pre-prepared glycosyl magnetic microsphere to obtain a high-affinity magnetic bead; carrying out molecular docking on the high-affinity magnetic beads and the aptamer structure, recording a docking score, and generating an optimized aptamer; recording the magnetic bead mass ratio of the optimized aptamer; capturing the natural killer cells by adopting the optimized aptamer, and shooting to obtain a fluorescent microscopic image; and recording the dyeing condition of the fluorescent microscopic image, determining the survival state of the NK cells, marking the position information of the living cells, and generating an NK cell delivery efficiency table by using the mass ratio of the magnetic beads. Based on a medical engineering technology, the homing rate and the delivery efficiency of the NK cells at the liver injury part are improved.

The present disclosure relates to the field of drug discovery and therapeutics, including systems and methods to predict drug function, to classify or prioritize drugs based on predicted function, and to test known and novel compounds for function in a vertebrate system. The present disclosure also relates to compounds identified using the disclosed systems and methods, the novel mechanisms of action of the compounds, and applications of the compounds as therapeutics, e.g., as cancer therapeutics.

This invention relates to the field of gene vector technology, and more particularly to an ionizable comb-like polymer, its preparation method, and its applications. This comb-like polymer is a block polymer, comprising at least one block unit. Each block unit includes a first block, a second block, and a third block linked by covalent bonds. It exhibits high molecular flexibility and readily binds to nucleic acid-like substances to form polymer / nucleic acid complexes. Due to the presence of easily degradable intracellular glycosidic bonds in its structure, the polymer demonstrates high transfection efficiency. Furthermore, cell experiments show that the polymer / nucleic acid complex exhibits low toxicity to cells and significantly improves cell survival compared to the positive control. Therefore, it holds promise for widespread application in cell transfection. The preparation method provided by this invention utilizes a redox amination tandem reaction to directly prepare the target product, eliminating the need for intermediate product purification processes. This simple process facilitates large-scale production.

This disclosure provides methods and compositions for genetically modifying lymphocytes, such as T cells and / or NK cells. [Solution] In some embodiments, the method comprises a reaction mixture produced using whole blood or its components other than PBMCs, and further comprising T cells and recombinant retroviral particles having polynucleotides encoding CARs, as well as the resulting cell preparation. In some embodiments, the modified lymphocytes are reintroduced subcutaneously into the subject. In some embodiments, polynucleotides are provided that confer to the T cells the ability to regulate cell survival and proliferation in response to binding to CARs.

The present invention provides a cell composition for treating detrusor underactivity and a method for preparing the same. The cell composition comprises a cell spheroid formed by seeding a plurality of mesenchymal stem cells in a culture device comprising a plurality of culture wells, wherein each of the culture wells has a surface coated with a low-protein-adhesion layer, and culturing the cells under the conditions to induce spheroid formation. Through the cell composition of the present invention, three-dimensional structured cell spheroids are formed to promote intercellular interactions and physiological function expression, thereby enhancing cell survival rates and functional performance after transplantation. In particular, the cell composition effectively facilitates the repair and restoration of contractile function of bladder muscle tissue, thereby improving the clinical symptoms associated with detrusor underactivity.

The invention discloses a sensitizing drug for resisting breast cancer by radiotherapy and a screening method and application thereof, and belongs to the field of biomedicine.The method comprises the steps that an in-vitro radiotherapy resistance model is constructed, a clinical segmentation radiotherapy dose increasing mode is simulated, stable acquired radiotherapy resistance is induced to be generated, and a carrier is provided for drug screening; natural plant small molecule compounds are used for treatment, and through concentration and time gradient dual control, the reversion ability of the natural plant small molecule compounds to radiotherapy resistance phenotypes is investigated; a multi-dimensional joint detection system is established, multi-level comprehensive evaluation is performed from cell survival and the like, the limitation of a single index is broken through, and the screening accuracy and reliability are improved; dNA damage repair is dynamically monitored through immunofluorescence, comet electrophoresis and the like, and the drug action mechanism is revealed. The medicine screened by the method is suitable for primary and acquired radiotherapy resistance patients, can effectively improve the radiotherapy curative effect, provides a new medicine source and scheme for clinical problems, and opens up the application of the compound in preparation of radiosensitizers or adjuvant therapy medicines.

The present application relates to the field of cell cryopreservation, and discloses a whole-process management method for cell cryopreservation, comprising the following steps: firstly, multi-dimensionally collecting cell survival indexes, screening high-quality cells in combination with a clustering algorithm, and completing standardized pretreatment; secondly, introducing a low-temperature tolerance response model algorithm to realize intelligent dynamic compounding of cryopreservation protectants; then, performing blockchain identity authentication and archiving on cell cryopreservation samples, and adopting an ice crystal targeting inhibition variable gradient freezing mode in combination with hierarchical intelligent storage management and control to ensure long-term storage stability of the samples; and finally, combining a time sequence evolution algorithm to perform activity attenuation prediction and periodic sampling evaluation on the stored samples, and dynamically grasping the storage state of the samples. The present application solves the technical defects of traditional manual cryopreservation, such as poor standardization, weak adaptability, low cell survival rate, sample management confusion, and inability to predict activity attenuation.

The present disclosure relates to manipulation of splicing of the Fas (tumor necrosis factor (TNF) receptor superfamily, member 6) gene in non-naïve cell of the T lineage for enhanced skipping of exon 6. The manipulated non-naïve cells predominantly express a soluble form of Fas (sFas) and display reduced expression of the membranal Fas (mFas), increased cytokine secretion, increased expression of activation markers, increased cell survival, increased cytotoxicity, and / or reduced expression of exhaustion markers. The present disclosure further provides specific splicing modulatory agents comprising gene editing systems and / or splice switching antisense oligonucleotide (SSO), methods and therapeutic uses thereof.

A method to prevent a disease, comprising: (1) administering a peptide comprising Delta6PV into a subject comprising a human or an animal; wherein the disease comprises one of more of Type 1 Diabetes, Rheumatoid arthritis, Lupus, Sjogren's syndrome, Multiple Sclerosis, Grave's disease, Giant cell artereitis, Ankylosing spondylitis, and Guillian Barre Syndrome; and (2) monitoring the disease in the subject.

The present application relates to a kind of enrichment method of stem cell high purity positive cell group, belong to the field of biotechnology and cell engineering technology.It aims to solve the technical problems such as the cell damage of existing screening technology, low screening efficiency, easy to affect stem cell pluripotency and genome stability, etc.The present application forms 23-cell small mass after H9 human embryonic stem cells infected by lentivirus are digested gently, after gradient dilution static culture, high expression positive clone is screened under fluorescence microscope, and target cell group is directly enriched by visual mechanical scraping.The present application does not change cell genome, and is high in safety;Small mass inoculation significantly improves cell survival and cloning efficiency at low density;Selective scraping operation is accurate, fast and low in cost;The proportion of reporter gene (EYFP) positive cells in the obtained positive cell group can reach 93.2%, and good pluripotency and function are maintained, suitable for efficient enrichment of stem cells and immune cells.

The invention provides wine gastrodia elata derived extracellular vesicles and application thereof in cerebral ischemia reperfusion injury, relates to the technical field of biological medicines, and provides application of the wine gastrodia elata derived extracellular vesicles in preparation of medicines for preventing and / or treating cerebral ischemia reperfusion injury. The traditional Chinese medicine composition can improve injury of nerve cells caused by oxygen deficit, inhibit phenotypic polarization of microglial cells to M1, promote phenotypic polarization of microglial cells to M2, reduce the area of cerebral infarction and improve neurological impairment after cerebral ischemia reperfusion. The invention reveals and verifies that the wine gastrodia elata-derived extracellular vesicle has an excellent nerve protection effect for the first time, and the vesicle can obviously relieve the injury of an anoxic environment to nerve cells, effectively maintain the cell activity and improve the survival ability of the cells in a stress state, so that the protection on brain tissue injury is realized from the cellular level.

The application discloses a kind of single-cell metabolite in vivo in situ detection method, which comprises the following steps: establishing single-cell cytoplasm sampling volume / single-cell volume relative quantification method, obtaining the relative maximum cytoplasm extraction volume when maintaining single-cell survival;Build composite ion source, obtain the minimum sampling volume of 150 metabolites detected from single cytoplasm, obtain the sampling volume of single-cell cytoplasm in the in vivo in situ detection of metabolome;Through picoliter pump probe microscope fixed-point positioning puncture cell membrane, extract the cell cytoplasm of the above sampling volume;Metabolites in the extracted single-cell cytoplasm are ionized using composite ion source;Metabolites after ionization are detected by mass spectrometry.The application can realize multiple single-cell cytoplasm extraction of in vivo embryo during development, mass spectrometry metabolite detection, while ensuring cell survival, multiple sampling, while realizing accurate detection of trace metabolites in single cell.

To provide compounds that prevent differentiation of cells and promote cell survival, including but not limited to, when the cells are isolated or are otherwise outside their normal medium or tissue milieu, in relation to compounds for stabilizing cells and their method of use.SOLUTION: Two classes of small molecular compounds shown in the selected drawing, by somewhat different mechanisms, both are useful as prophylactic and therapeutic compounds for a number of different disease indications, including but not limited to, cancer, tissue damage, and stroke.SELECTED DRAWING: Figure 1A

The invention relates to a mutton sheep frozen semen protective agent and a preparation method and application method thereof, and the mutton sheep frozen semen protective agent comprises a multi-pillar synergistic protection system, the protection system comprises a basic buffer system, an energy and impermeable protection system, a penetrable cryoprotection system, a mitochondrial targeting protection system, an epigenetic regulation system, a biological nano composite system, a membrane stabilization and cell survival signal regulation system and an auxiliary system.

The application belongs to the technical field of hydrogel and provides a dynamic cross-linking hydrogel and a preparation method and application thereof, and comprises the following steps: cross-linking oxidized dextran and carboxymethyl chitosan containing a phenylboronic acid modified group; adding dextranase before hydrogel formation, and introducing polydopamine-coated calcium peroxide nanoparticles; wherein the mass concentration of the oxidized dextran is 2-10% w / v, the mass concentration of the carboxymethyl chitosan containing the phenylboronic acid modified group is 2% w / v, the dextranase concentration is 10-100 U, and the concentration of the polydopamine-coated calcium peroxide nanoparticles is 0.1-1% w / v. The hydrogel of the application shows good cell compatibility, pro-angiogenic capacity and biological activity for promoting dental pulp regeneration in a plurality of in-vitro and in-vivo experiments, is suitable for the field of tissue engineering such as dental pulp injury repair, and effectively solves technical problems such as insufficient cell survival support of conventional hydrogels in three-dimensional culture.

The invention belongs to the technical field of biological medicines, and particularly discloses a polypeptide for protecting lung vascular endothelial cells and relieving lung injury and application of the polypeptide. The polypeptide is Humanin-G (HNG), and the amino acid sequence of the polypeptide is MAPRGFSCLLLLTGEIDLPVKRRA. The polypeptide is targeted to mitochondria of pulmonary vascular endothelial cells, provides protection from energy supply and cell survival roots, and has profound significance in improving functions of the pulmonary vascular endothelial cells and reducing the death rate of sepsis-induced acute lung injury (ALI). Researches show that when the polypeptide is used in the acute injury stage, lung inflammation of a model animal can be relieved by 25%-50%, the lung injury HE score can be reduced by about 50%, pulmonary edema can be relieved by about 30%, mitochondrial functions can be recovered by about 70%, the ATP yield can be increased by 30%, and the effect is excellent. Therefore, the invention provides a brand new strategy for maintaining the mitochondrial homeostasis by using the polypeptide so as to relieve lung injury.

The invention provides a traditional Chinese medicine composition for preventing, treating and / or improving brain diseases directly related to brain microvascular endothelial cells and application of the traditional Chinese medicine composition, and belongs to the technical field of traditional Chinese medicine compositions. The traditional Chinese medicine composition is prepared from the following raw materials in parts by weight: ginseng under forest for more than 10 years, fructus lycii, fructus rosae laevigatae, rhizoma polygonati, semen cuscutae, wine-processed glossy privet fruit, radix paeoniae alba, herba epimedii, honey-fried licorice root, honey-fried astragalus membranaceus, fried malt and honey. The honey-fried licorice root is prepared from wild licorice root; the radix astragali preparata is prepared by processing high-quality organic radix astragali. According to the traditional Chinese medicine composition, the brain microvascular endothelial cell protection activity of the traditional Chinese medicine composition can be effectively improved by improving the source and grade of key medicinal materials of the traditional Chinese medicine composition; the traditional Chinese medicine composition disclosed by the invention is more outstanding in the aspects of relieving oxidative damage, improving the cell survival state and reducing oxidative stress and apoptosis.

A human Vγ9Vδ2T cell proliferation method and a culture medium. The method comprises: first stimulating a human Vγ9Vδ2T cell with a culture medium containing interleukin-2 and phosphonic acid compounds, and then culturing the human Vγ9Vδ2T cell with a culture medium added with interleukin-15 and vitamin C to achieve proliferation culture. The culture medium additionally comprises interleukin-15 and vitamin C. Compared with a conventional proliferation method and a conventional culture medium, the method and the culture medium can improve the proliferation efficiency and cell purity of the human Vγ9Vδ2T cell. The human Vγ9Vδ2T cell obtained by culture in the method has stronger anti-apoptotic ability and longer cell survival time, and moreover, the expression level of critical killer molecules NKG2D thereof is higher, thereby having stronger killing ability to tumor cells.

This invention belongs to the field of chemical medicine, specifically relating to a phenylpropanoid compound, its preparation method, and its application. The phenylpropanoid compound specifically refers to the compound, solvate, optically pure isomer, stereoisomer, or pharmaceutically acceptable salt thereof, or mixtures thereof, represented by general formula (Ⅰ), wherein X is selected from O or N; R1, R2, R3, R4, and R5 are the same or different, and are independently selected from hydrogen, hydroxyl, halogen, alkoxy, and alkylthio groups; R 13 Selected from hydrogen or C1-C4 alkyl, or R 13 Connect R5 to form a loop; R 14 The compounds are selected from cycloalkyl groups with 5-6 substituted carbon atoms. The phenylpropanoid compounds synthesized in this invention exhibit significant inhibitory effects on glioma cells, and their inhibitory effect is superior to that of the positive control drugs temozolomide and the original chlorogenic acid. Simultaneously, some of the synthesized phenylpropanoid compounds significantly improve neuronal cell survival rates, and their protective effect is superior to that of the positive control drug edaravone, demonstrating potential for treating stroke.

The invention relates to the technical field of biology, and discloses a composition for enhancing the immunomodulatory function of stem cells, and the composition comprises a ferrous ion source, a ferroptosis inhibitor and L-arginine in a basic culture medium. According to the method, a substrate (L-arginine) synthesized by nitric oxide (NO), a necessary cofactor (divalent iron ions) for activating a key enzyme iNOS and a ferroptosis inhibitor are connected in parallel to avoid the toxicity of iron ions and guarantee cell survival, the three components have a synergistic effect, an optimized intracellular environment is constructed, and the method can be used for preparing an anti-inflammatory drug under the condition of not depending on the stimulation of inflammatory factors. The immunoregulation function of the stem cells is safely and efficiently enhanced. By using the composition disclosed by the invention, the treatment efficacy of the stem cells can be remarkably improved, meanwhile, the immunogenicity of the stem cells is effectively prevented from being improved, and a new technical path is provided for developing safer cell treatment products.

Described are methods, devices, and systems for measuring cellular responses to differential stimuli using one or more analyzers (e.g., optical, fluidic / hydrodynamic, electrokinetic, magnetic and / or acoustic force-based analyzers). The methods may include receiving a selection of an initial samples (e.g., containing treated biological cells), performing measurements (e.g., force-based measurements) on the samples, and at least one of: developing a metric to describe the cellular fingerprint based on one or more optical or fluidic force-based parameters, or correlating the cellular response to a cellular property of interest (e.g., cell viability and / or vitality).

The present invention generally relates to a therapeutic hydrogel device. More particularly, the present invention describes various embodiments of a hydrogel macrodevice, such as a planar hybrid hydrogel macrodevice that can achieve spatially controlled distribution of microtissues and support establishment of intra-device vasculature for enhanced cell survival, and individually encapsulated microtissues, and methods of use.