87 results about "Tissue defect" patented technology

The invention provides an injectable short-fiber-based hydrogel tissue repair material with an electric response characteristic as well as a preparation method and application thereof, and the injectable short-fiber-based hydrogel tissue repair material with the electric response characteristic is prepared by taking a natural polymer material loaded with bioactive factors as a core layer material and a biodegradable material as a shell layer material through a coaxial electrostatic spinning technology. The method comprises the following steps: preparing short fibers with a core-shell structure, uniformly mixing the short fibers with a hydrogel matrix, injecting and filling a tissue defect part, and carrying out ultraviolet curing to form the tissue repair material. The injectable short-fiber-based hydrogel tissue repair material with the electrical response characteristic provided by the invention has piezoelectric characteristic and injectable characteristic, can generate electrical response through mechanical stimulation so as to promote cell proliferation and tissue repair, and also has controllable drug release capacity and good mechanical adaptability; the method is suitable for application scenes of complex tissue damage repair such as diabetes infected wound repair, articular cartilage repair, oral barrier membrane repair, abdominal wall defect repair and the like.

The present invention relates to the field of medical devices, and particularly to a closure device for tissue defects. The closure device includes a locking unit and a release unit located in the proximal region of the locking unit; the locking unit includes at least one or more locking members; by operating the release unit, the locking members are driven to rotate, the locking members approach each other and achieve self-locking, thereby realizing the closure of the tissue defect; the locking unit and the release unit are of an integral structure, and when the locking members achieve self-locking, further operating the release unit causes the release unit to break away from the locking unit.

The invention discloses hydrogel for repairing volumetric muscle defects and a preparation method of the hydrogel, and the hydrogel is prepared from oxidized dextran, an alcohol-polyamino acid-bilirubin polymer and 3, 3 '-dithio-bis (propionyl hydrazide) according to the weight ratio of (50-75): (4-20): 1. The alcohol-polyamino acid-bilirubin polymer is prepared from an alcohol-polyamino acid polymer and bilirubin in a weight ratio of (6-25): 1. The hydrogel prepared by the invention not only has good characteristics of pH / ROS responsiveness, relatively strong tissue adhesion, antibacterial property, oxidation resistance and relatively low cytotoxicity, but also can remarkably promote cell migration, proliferation, new angiogenesis and myoblast differentiation, promote recovery of muscle structures and motor functions after volumetric muscle defects, and improve the recovery rate of muscle tissues and motor functions after the volumetric muscle defects. The material is expected to become a novel bioactive material capable of promoting volume muscle defect repair, and has a good application prospect in clinical treatment of large-volume tissue defect regeneration.

The invention discloses bi-crosslinking self-reinforced hydrogel based on endogenous glucose in-situ initiation as well as a preparation method and application of the bi-crosslinking self-reinforced hydrogel, and relates to the technical field of biomedical materials. The double-crosslinking self-enhanced hydrogel is prepared from the following components in concentration: 1 to 3 weight percent of hyaluronic acid-tyramine, 0.8 to 1.2 weight percent of sodium alginate, 1 to 100 mmol / L of calcium carbonate, 0.0001 to 10 U / mL of horse radish peroxidase, 20 to 40 U / mL of glucose oxidase and the balance of ultrapure water. The invention also discloses an application of the double-crosslinking self-reinforced hydrogel in preparation of a filling and repairing product for soft tissue defects. The hydrogel disclosed by the invention can be filled in a tissue defect part in an injection manner, glucose in body fluid is utilized to initiate crosslinking in situ to form the hydrogel with excellent mechanical properties and long-term stability, and meanwhile, the hydrogel is good in biocompatibility and relatively high in application value.

The invention discloses application of a tumor-derived acellular extracellular matrix in preparation of a medicine for promoting angiogenesis and tissue regeneration. The invention provides a strategy for regulating and controlling tissue regeneration based on a tumor-derived acellular extracellular matrix microenvironment, a novel angiogenesis promoting biological material is constructed by combining the high similarity of a tumor microenvironment and a regeneration microenvironment such as angiogenesis, and high-efficiency regeneration is realized in various tissue defects. In addition, due to the characteristic of rapid proliferation of the tumor tissue, the productivity bottleneck of a traditional acellular extracellular matrix raw material source is broken through, and sustainable biological raw materials are provided for large-scale production. The method successfully solves the problem that a specific tissue-derived acellular extracellular matrix is difficult to regenerate in a complex damaged microenvironment.

The present invention discloses a recombinant collagen and its use in cartilage repair matrix. The recombinant collagen provided by the present invention comprises a sequence shown in SEQ ID No.1; the amino acid sequence of the recombinant collagen comprises N basic repeating units, and the basic repeating unit contains n1 of the following characteristic amino acid sequences: "G‑Xaa1‑Xaa2‑G‑E‑Xaa3"; the 3' end and the 5' end of the basic repeating unit are connected to form the above-mentioned characteristic amino acid sequence. The recombinant collagen provided by the present invention has a relatively obvious integrin binding activity, has the effects of promoting cell adhesion, proliferation, differentiation, repairing cartilage tissue defects, etc., and has good application prospects.

The present disclosure relates generally to devices and methods for treating a tissue defect, for example, by suturing. In some embodiments, a suturing device may include an elongate member having a working channel, a suture channel, and a suture arm extending from the elongate member. The suturing device may further include a needle passer located within the working channel, the needle passer operable to deliver a needle between the elongate member and a distal end of the suture arm for suturing a target tissue, and a suture extending through the suture channel, wherein the suture is coupled to the needle. The suturing device may further include a plurality of imaging devices, wherein a first imaging device is positioned along a distal face of the elongate member, and wherein a second imaging device is positioned along the suture arm.

The invention discloses an instant plugging agent as well as a preparation method and application thereof, and belongs to the technical field of biological materials. The instant plugging agent comprises a solution A and a solution B, wherein the solution A is an organic solution containing lipoic acid; the solution B is an aqueous solution containing metal ions. The preparation method comprises the following steps: dissolving lipoic acid in an organic solvent to prepare a solution A; dissolving salt of metal ions in water to prepare a solution B; the application refers to the application in preparation of products for stopping bleeding of human bodies or animal bodies and / or sealing tissue defects. The plugging agent has the characteristic of instant curing, and can tolerate bursting pressure exceeding 450 mm Hg; the device is not only suitable for general wound errhysis, but also can effectively deal with acute hemorrhage and emergency plugging of tissue defects in high-pressure and high-flow-rate states.

A medical device for approximating and securing tissue without requiring knots includes a lock-head, a strap section, distal protuberance, and a leader section. The device also includes a transition section between the leader and the strap section and a stiffening section proximal to the protuberance. The leader section is used to draw the strap section into the body through small apertures in tissue and the transition section provides a gradual transition in stiffness and size between the leader and the strap section.

The present invention provides a biodegradable, injectable adhesive bone cement for osteoporosis, its preparation method and application. The composition for preparing the injectable bone cement comprises a liquid phase component and a solid phase component, wherein the liquid phase component includes a polyester polymer; the solid phase component includes a phosphate or a phosphate and its derivatives. The injectable bone cement is formed by cross-linking reaction of a polyester polymer, a phosphate and a phosphate derivative, and can be injected, biodegradable, adhesive to bone tissue, capable of regulating the bone microenvironment and treating osteoporosis. The biosafe bone cement of the present invention can highly fit with complex tissue defect sites, has no obvious heat release phenomenon during the curing process, can achieve strong adhesion to bone tissue, and its composition and structure can promote bone tissue repair under osteoporosis pathological conditions and improve the quality of bone repair. It is a bone tissue repair material with clinical application prospects.

The application relates to the field of medical devices, in particular to a closure device with positioning and guiding puncture functions, which comprises a delivery catheter, an implant for closing a tissue defect, and a puncture mechanism and a positioning member arranged in the delivery catheter, the puncture mechanism can puncture the tissue to be closed back and forth, the positioning member comprises a connecting member and a positioning support arranged at the distal end of the connecting member, and the positioning support can provide a supporting force for the puncture mechanism when puncturing the tissue to be closed back and forth; in the application, the positioning support can provide bidirectional support for the puncture mechanism when puncturing the tissue back and forth, the puncture precision is improved, the tissue can be prevented from being damaged, and the application has good clinical significance.

The application discloses application of tumor-derived acellular extracellular matrix in preparation of a drug for promoting angiogenesis and tissue regeneration. The application proposes a strategy for regulating tissue regeneration based on a tumor-derived acellular extracellular matrix microenvironment, combines the high similarity between a tumor microenvironment and a regeneration microenvironment such as angiogenesis, and constructs a novel angiogenesis-promoting biomaterial, so that high-efficiency regeneration is realized in various tissue defects. In addition, the characteristics of rapid proliferation of tumor tissues break through the production capacity bottleneck of a raw material source of a traditional acellular extracellular matrix, and provide sustainable biological raw materials for large-scale production. The application successfully solves the problem that a specific tissue-derived acellular extracellular matrix is difficult to regenerate in a complex damage microenvironment.

The present invention relates to a method of producing a collagen membrane that has particular mechanical properties. In particular, the present invention relates to a method A of producing a collagen membrane comprising the steps of (i) isolating a collagen-containing tissue and incubating same in an ethanol solution; (ii) incubating the collagen-containing tissue from step (i) in a first solution comprising an inorganic salt and an anionic surfactant in order to denature non-collagenous proteins contained therein; (iii) incubating the collagen-containing tissue produced in step (ii) in a second solution comprising an inorganic acid until the collagen in said material is denatured; and (iv) incubating the collagen-containing tissue produced in step (iii) in a third solution comprising an inorganic acid with simultaneous mechanical stimulation for sufficient time to enable the collagen bundles in said collagen-containing tissue to align; wherein the mechanical stimulation comprises applying tension cyclically to the collagen-containing tissue.

A tissue repair device, system, and method for grasping tissue, such as to repair the tissue (e.g., a defect in tissue), by anchoring a tissue-penetrating arm along a first location along the tissue, moving the anchored tissue-penetrating arm to a second location along the tissue, and grasping tissue at the second location with a tissue-grasping arm. The tissue-penetrating arm remains in a substantially axial straight configuration to facilitate tissue penetration. The tissue-grasping arm is movable towards and away from the tissue-penetrating arm to grasp tissue and hold the grasped tissue between the tissue-grasping arm and the tissue-penetrating arm. The tissue repair device may be left in place holding the tissue in the grasped configuration.

The present application relates to a kind of tissue defect plugging device and tissue defect plugging device.Tissue defect plugging device includes plugging device body, plugging device body is memory material.When plugging device body is in natural relaxed state, all clamping ring is in the same plane, or, adjacent two clamping ring is provided with gap in the direction of straight line perpendicular to its plane, can produce original shape restoring torque, so that the first atrial septum and the second atrial septum produce mutual close tendency, and then make the first atrial septum and the second atrial septum mutually close and close, finally reach the purpose of closing foramen ovale, plugging effect is better, while operation is relatively simple.In addition, because the caliber of clamping ring is different, so as to cancel the flow blocking film, simple structure, also obtain the effect of plugging foramen ovale;In addition, using the above structure design form, the metal material implanted in body is less, can avoid the complication of thrombosis, i.e. not easy to cause adverse reaction.

This invention discloses a microgel-assembled bilayer scaffold for integrated osteochondral repair and its preparation method. The preparation method includes the following steps: (1) synthesizing gelatin modified with carbon-carbon double bonds and dopamine; (2) preparing modified gelatin composite microgels loaded with bioactive glass and chondroitin sulfate respectively; (3) preparing hyaluronic acid modified with carbon-carbon double bonds and phenylboronic acid; (4) suspending the modified gelatin composite microgels loaded with bioactive glass and the modified gelatin composite microgels loaded with chondroitin sulfate in modified hyaluronic acid solutions respectively, and loading them into two different syringes; (5) injecting the two microgel suspensions sequentially into a mold, and constructing the microgel-assembled bilayer scaffold in situ under ultraviolet light. The scaffold of this invention has an open porous structure and good cell compatibility, which can promote the bidirectional differentiation of bone marrow mesenchymal stem cells into chondrogenic and osteogenic cells, and can be used for integrated regeneration and repair of osteochondral tissue defects.

This application relates to the field of medical devices, and more particularly to a tissue puncture closure device with a control device, including a delivery system and an implant for closing tissue defects. The control device includes a connection control mechanism and a guide control mechanism. The implant includes a blocking member and a connector connected to the blocking member. The delivery system includes a puncture member and a pusher member for pushing the blocking member. The connection control mechanism includes a first connecting structure disposed on the blocking member and a second connecting structure disposed on the pusher member and detachably connected to the first connecting structure. The blocking member and the pusher member are slidably sleeved within the puncture member. The guide control mechanism includes a first guide structure disposed on the distal portion of the puncture member and a second guide structure disposed on the blocking member and / or the pusher member. The connection control mechanism and the guide control mechanism respectively restrict the axial movement and circumferential rotation of the blocking member relative to the puncture member, ensuring that the blocking member does not prematurely detach from the puncture member or rotate arbitrarily.

The invention discloses a macrophage hybrid scaffold as well as a construction method and application thereof, and belongs to the technical field of biological materials. The construction method comprises the following steps: extracting peripheral blood mononuclear cells, inducing the peripheral blood mononuclear cells into mature macrophages, loading the mature macrophages on a tissue engineering scaffold, and adding an immunoregulation factor to regulate the phenotype of the mature macrophages to obtain the M2 type macrophage hybrid scaffold. According to the invention, the macrophages with an immunoregulation effect are introduced into the tissue engineering scaffold, the macrophage hybrid scaffold is constructed together, and the macrophage hybrid scaffold can be used for repairing tissue defects, such as defect tissues such as skin, mucous membrane, viscera, cartilage or hard bone and the like; the macrophage hybrid scaffold can meet various requirements of providing mechanical support and bioremediation functions for tissue defect areas.

Local tissue defects, liquefaction, infection, and necrosis of skin resulted from various reasons may lead to skin damage and vascular regeneration disorders. Wound hypoperfusion, metabolic disorders, and delayed epithelial cell formation disrupt the process of normal wound healing, thereby leading to the formation of chronic ulcer. To solve the aforementioned problems, a use of polymer dot is provided. The polymer dot may be used for manufacture of a component for the treatment of skin to a subject in need, in order to restore skin damage of the subject, minimize a scar on the damaged skin of the subject, and enhance recovery of the skin of the subject after cosmetic or dermatologic surgery. The polymer dot includes a carbon dot.

The application relates to the field of medical devices, in particular to a closed system with a supporting function, which comprises a first delivery tube, an implant for closing a tissue defect, and a puncture assembly and a second delivery tube arranged in the first delivery tube, the puncture assembly can reciprocally puncture the tissue to be closed, the second delivery tube is provided with a first connecting rod and a supporting device arranged at the distal end of the first connecting rod, the supporting device has a preset shape, wherein, when the supporting device restores to the preset shape, the supporting device forms a first supporting area and a second supporting area, and the first supporting area and the second supporting area respectively provide support for the puncture assembly when reciprocally puncturing the tissue to be closed; in the application, the supporting device is simple in structure, large in supporting area and easy to recycle.

This invention provides a calcium phosphate-polycaprolactone composite scaffold, its preparation method, and its applications. The raw materials for preparing the calcium phosphate-polycaprolactone composite include polycaprolactone, β-tricalcium phosphate, and hydroxyapatite. The resulting composite scaffold possesses functions of promoting immunity and regulating bone regeneration. The preparation method of this invention involves first forming a hydroxyapatite / polycaprolactone composite film, which is then broken down and recombined with β-tricalcium phosphate to form a composite solution. The resulting 3D-printed composite scaffold not only possesses good mechanical strength and biocompatibility but also induces stem cell proliferation and osteogenic differentiation, and induces macrophage regeneration and polarization. It can be used to regulate immune cell responses, induce a bone immune microenvironment conducive to bone formation, thereby enhancing bone regeneration, and can be used for the repair and regeneration of tissue defects.

The present application relates to the field of medical materials, in particular to a preparation method of high-performance medical tantalum coating. A thin-size-low-diffusion tantalum coating is prepared on the surface of a porous titanium alloy substrate by a chemical vapor deposition method through a halide reaction of hydrogen reduction of metallic tantalum; there is an alloy element interdiffusion zone between the tantalum coating and the porous titanium alloy substrate, and the thickness of the interdiffusion zone is less than the thickness of the tantalum coating. The present application obtains a high-performance thin-size tantalum coating with high purity, small grain size and controllable transition layer suitable for the porous titanium alloy substrate, has higher preparation efficiency and lower cost, realizes high bonding strength of the coating and simultaneous improvement of the strength and plasticity of the porous titanium alloy, avoids dissolution of toxic metal ions such as Al and V, and improves biocompatibility and osteogenic performance. The new tantalum coating porous titanium alloy implant device has obvious advantages of higher mechanical, chemical and biological safety and lower cost, and is suitable for the fields of orthopedics, dental implants and tissue defect fillers.

An injectable bifunctional hydrogel has antibacterial activity. The bifunctional hydrogel is a hydrogel prepared by dissolving nucleoside analogues in a solvent. The nucleoside analog has a structure of formula I. It can be a candidate drug for the local injection treatment of periodontal disease, and it also has potential application prospects in the minimally invasive treatment of craniofacial bone tissue defects.

The invention discloses a preparation method and application of a GS-loaded mesenchymal stem cell exosome, and belongs to the technical field of biology. In order to solve the problems of slow onset and long recovery period of a diabetic skin tissue defect healing method in the prior art, the invention provides a preparation method of a GS-loaded mesenchymal stem cell exosome, the yield of the MSCs exosome is induced to be greatly increased through high-frequency ultrasonic stimulation and a microfluidic circulation technology, and the GS-loaded mesenchymal stem cell exosome is prepared. An original cell membrane structure is broken by using an acoustic fluid nano directional coupling technology, phospholipid bimolecules are driven to aggregate and self-assemble to form exosomes which are wrapped in GS, and effective drug delivery and synergistic tissue repair are realized; according to the method, nucleic acid, protein, saccharide, polypeptide and various small molecule compounds can be loaded in the exosome, and certain universality is achieved. The GS-loaded mesenchymal stem cell exosome provided by the invention can be applied to preparation of drugs for promoting healing of skin tissue defects and promoting regeneration of diabetic skin tissues.

Compositions comprising a condensed mesenchymal cell body and a hydrogel are provided. The compositions may further include drugs or growth factors. The condensed mesenchymal cell body may include a connective tissue cell, or even a progenitor cell capable of producing connective tissue extracellular matrices such collagen and glycosaminoglycan. Also provided are methods of treating connective tissue defects, cartilage injury, and cartilage degradation.

A medical device for approximating and securing tissue without the need for knots includes a locking head, a strap portion, a distal ridge, and a leader portion. The device also includes a transition portion between the leader and strap portion and a reinforcing portion proximal to the ridge. The leader portion is used to pull the strap portion through a small opening in the tissue and into the body, and the transition portion provides a gradual transition in stiffness and size between the leader and strap portion. A tensioner and cutter are provided, as well as a threading tool configured to be slidably removed from the lumens of the tensioner and cutter. A blade is provided within the lumens of the tensioner and cutter for cutting off excess leader and / or strap when the procedure is complete.

A medical device for approximating and securing tissue without requiring knots includes a lock-head, a strap section, distal protuberance, and a leader section. The device also includes a transition section between the leader and the strap section and a stiffening section proximal to the protuberance. The leader section is used to draw the strap section into the body through small apertures in tissue and the transition section provides a gradual transition in stiffness and size between the leader and the strap section. Tensioner and cutter and a threading tool configured to be slidably removed from a lumen of the tensioner and cutter is provided. A blade within the lumen of the tensioner and cutter is provided to cut excess leader and / or strap when the procedure is completed.

The invention relates to a bionic bone scaffold customized body and a preparation method and application thereof, and belongs to the technical field of biomedical materials. The invention provides a preparation method of a bionic bone scaffold customized body, which comprises the following steps: scanning and recording a cranio-maxillofacial hard tissue defect form, and constructing a digital scaffold model through an algorithm model and / or artificial design based on the recorded data; performing three-dimensional reconstruction on the obtained digital scaffold model to generate bionic bone scaffold customized volume data meeting the personalized requirements of cranio-maxillofacial tissue defects; and converting the obtained customized body data of the bionic bone scaffold into a customized body of the bionic bone scaffold by utilizing a 3D printing technology. Modeling is conducted through real bone defect data, a bionic bone scaffold customized body of the bone defect part is prepared through the 3D printing technology, the outer contour is attached to the bone defect side form, the ideal bone form is reconstructed, the inner contour is a fine substructure of the defect part, and the bionic bone scaffold has a bionic pore structure and biomechanical performance; the method can be used for reconstructing personalized cranio-maxillofacial tissues.

It is an object of the present disclosure to provide a novel dental preparation for use in the treatment of a tissue defect. The dental preparation contains a shaped body obtained by drying a raw material aqueous solution containing collagen and / or gelatin (A), a free form and / or alkali metal salt of bromfenac (B), and a water-soluble divalent metal salt (C).

The invention discloses an injectable intelligent interface hydrogel microsphere with sequential controlled release as well as a preparation method and application of the injectable intelligent interface hydrogel microsphere. The microspheres are prepared from methacrylated gelatin, carboxymethyl cellulose nanocellulose and brain-derived neurotrophic factors, and the microspheres are treated by chitosan, so that the microspheres are shrunk and loaded with more brain-derived neurotrophic factors at the same time. On the basis, through polydopamine modification, the obtained microspheres have a sequential controlled release effect, and are beneficial to improving local inflammation after tissue defect and promoting regeneration and repair of defect tissues. In addition, the microspheres also have injectable characteristics, so that the microspheres can be conveniently applied to defect parts needing to be repaired. Acute-stage inflammatory response and subacute-stage and chronic-stage nerve fiber cell response can be regulated and controlled by utilizing the action of the sequential release biological factors of the microspheres and the multi-layer space structure, and spinal cord regeneration is promoted.