28 results about "Tissue defect" patented technology

The invention discloses bi-crosslinking self-reinforced hydrogel based on endogenous glucose in-situ initiation as well as a preparation method and application of the bi-crosslinking self-reinforced hydrogel, and relates to the technical field of biomedical materials. The double-crosslinking self-enhanced hydrogel is prepared from the following components in concentration: 1 to 3 weight percent of hyaluronic acid-tyramine, 0.8 to 1.2 weight percent of sodium alginate, 1 to 100 mmol / L of calcium carbonate, 0.0001 to 10 U / mL of horse radish peroxidase, 20 to 40 U / mL of glucose oxidase and the balance of ultrapure water. The invention also discloses an application of the double-crosslinking self-reinforced hydrogel in preparation of a filling and repairing product for soft tissue defects. The hydrogel disclosed by the invention can be filled in a tissue defect part in an injection manner, glucose in body fluid is utilized to initiate crosslinking in situ to form the hydrogel with excellent mechanical properties and long-term stability, and meanwhile, the hydrogel is good in biocompatibility and relatively high in application value.

The invention discloses an instant plugging agent as well as a preparation method and application thereof, and belongs to the technical field of biological materials. The instant plugging agent comprises a solution A and a solution B, wherein the solution A is an organic solution containing lipoic acid; the solution B is an aqueous solution containing metal ions. The preparation method comprises the following steps: dissolving lipoic acid in an organic solvent to prepare a solution A; dissolving salt of metal ions in water to prepare a solution B; the application refers to the application in preparation of products for stopping bleeding of human bodies or animal bodies and / or sealing tissue defects. The plugging agent has the characteristic of instant curing, and can tolerate bursting pressure exceeding 450 mm Hg; the device is not only suitable for general wound errhysis, but also can effectively deal with acute hemorrhage and emergency plugging of tissue defects in high-pressure and high-flow-rate states.

The application relates to the field of medical devices, in particular to a closure device with positioning and guiding puncture functions, which comprises a delivery catheter, an implant for closing a tissue defect, and a puncture mechanism and a positioning member arranged in the delivery catheter, the puncture mechanism can puncture the tissue to be closed back and forth, the positioning member comprises a connecting member and a positioning support arranged at the distal end of the connecting member, and the positioning support can provide a supporting force for the puncture mechanism when puncturing the tissue to be closed back and forth; in the application, the positioning support can provide bidirectional support for the puncture mechanism when puncturing the tissue back and forth, the puncture precision is improved, the tissue can be prevented from being damaged, and the application has good clinical significance.

This application relates to the field of medical devices, and more particularly to a tissue puncture closure device with a control device, including a delivery system and an implant for closing tissue defects. The control device includes a connection control mechanism and a guide control mechanism. The implant includes a blocking member and a connector connected to the blocking member. The delivery system includes a puncture member and a pusher member for pushing the blocking member. The connection control mechanism includes a first connecting structure disposed on the blocking member and a second connecting structure disposed on the pusher member and detachably connected to the first connecting structure. The blocking member and the pusher member are slidably sleeved within the puncture member. The guide control mechanism includes a first guide structure disposed on the distal portion of the puncture member and a second guide structure disposed on the blocking member and / or the pusher member. The connection control mechanism and the guide control mechanism respectively restrict the axial movement and circumferential rotation of the blocking member relative to the puncture member, ensuring that the blocking member does not prematurely detach from the puncture member or rotate arbitrarily.

This invention provides a calcium phosphate-polycaprolactone composite scaffold, its preparation method, and its applications. The raw materials for preparing the calcium phosphate-polycaprolactone composite include polycaprolactone, β-tricalcium phosphate, and hydroxyapatite. The resulting composite scaffold possesses functions of promoting immunity and regulating bone regeneration. The preparation method of this invention involves first forming a hydroxyapatite / polycaprolactone composite film, which is then broken down and recombined with β-tricalcium phosphate to form a composite solution. The resulting 3D-printed composite scaffold not only possesses good mechanical strength and biocompatibility but also induces stem cell proliferation and osteogenic differentiation, and induces macrophage regeneration and polarization. It can be used to regulate immune cell responses, induce a bone immune microenvironment conducive to bone formation, thereby enhancing bone regeneration, and can be used for the repair and regeneration of tissue defects.

The present application relates to the field of medical materials, in particular to a preparation method of high-performance medical tantalum coating. A thin-size-low-diffusion tantalum coating is prepared on the surface of a porous titanium alloy substrate by a chemical vapor deposition method through a halide reaction of hydrogen reduction of metallic tantalum; there is an alloy element interdiffusion zone between the tantalum coating and the porous titanium alloy substrate, and the thickness of the interdiffusion zone is less than the thickness of the tantalum coating. The present application obtains a high-performance thin-size tantalum coating with high purity, small grain size and controllable transition layer suitable for the porous titanium alloy substrate, has higher preparation efficiency and lower cost, realizes high bonding strength of the coating and simultaneous improvement of the strength and plasticity of the porous titanium alloy, avoids dissolution of toxic metal ions such as Al and V, and improves biocompatibility and osteogenic performance. The new tantalum coating porous titanium alloy implant device has obvious advantages of higher mechanical, chemical and biological safety and lower cost, and is suitable for the fields of orthopedics, dental implants and tissue defect fillers.

An injectable bifunctional hydrogel has antibacterial activity. The bifunctional hydrogel is a hydrogel prepared by dissolving nucleoside analogues in a solvent. The nucleoside analog has a structure of formula I. It can be a candidate drug for the local injection treatment of periodontal disease, and it also has potential application prospects in the minimally invasive treatment of craniofacial bone tissue defects.

Compositions comprising a condensed mesenchymal cell body and a hydrogel are provided. The compositions may further include drugs or growth factors. The condensed mesenchymal cell body may include a connective tissue cell, or even a progenitor cell capable of producing connective tissue extracellular matrices such collagen and glycosaminoglycan. Also provided are methods of treating connective tissue defects, cartilage injury, and cartilage degradation.

A medical device for approximating and securing tissue without requiring knots includes a lock-head, a strap section, distal protuberance, and a leader section. The device also includes a transition section between the leader and the strap section and a stiffening section proximal to the protuberance. The leader section is used to draw the strap section into the body through small apertures in tissue and the transition section provides a gradual transition in stiffness and size between the leader and the strap section. Tensioner and cutter and a threading tool configured to be slidably removed from a lumen of the tensioner and cutter is provided. A blade within the lumen of the tensioner and cutter is provided to cut excess leader and / or strap when the procedure is completed.

The invention relates to a bionic bone scaffold customized body and a preparation method and application thereof, and belongs to the technical field of biomedical materials. The invention provides a preparation method of a bionic bone scaffold customized body, which comprises the following steps: scanning and recording a cranio-maxillofacial hard tissue defect form, and constructing a digital scaffold model through an algorithm model and / or artificial design based on the recorded data; performing three-dimensional reconstruction on the obtained digital scaffold model to generate bionic bone scaffold customized volume data meeting the personalized requirements of cranio-maxillofacial tissue defects; and converting the obtained customized body data of the bionic bone scaffold into a customized body of the bionic bone scaffold by utilizing a 3D printing technology. Modeling is conducted through real bone defect data, a bionic bone scaffold customized body of the bone defect part is prepared through the 3D printing technology, the outer contour is attached to the bone defect side form, the ideal bone form is reconstructed, the inner contour is a fine substructure of the defect part, and the bionic bone scaffold has a bionic pore structure and biomechanical performance; the method can be used for reconstructing personalized cranio-maxillofacial tissues.

This application provides an endoscopic tissue closure device, including a working part, a transmission part, and a control handle. The working part includes a claw assembly, a flexible fitting wing, a tightening tube, a front end tube, and a traction assembly. The tightening tube and the front end tube are detachably connected to form a movable cavity, and the traction assembly is placed in the movable cavity. At least three elastic claws of the flexible fitting wing and the claw assembly are tightened at the front end of the tightening tube, and the elastic claws surround the outer side of the flexible fitting wing. The transmission part connects the front end tube and the control handle. The traction assembly includes a first traction mechanism and a second traction mechanism. The control handle controls the extension and retraction of the flexible fitting wing and the claw assembly through the two traction mechanisms respectively. After the flexible fitting wing extends, it releases and unfolds, abutting against the outer surface of the wound. After the claw assembly extends, it elastically opens to grasp the edge of the inner wound, and closes under the action of the tightening tube when it retracts. Through the coordinated cooperation of the fitting wing and the claws, precise coverage and all-round grasping of tissue defects are achieved, improving the reliability of closure and simplifying the operation.

The application provides a microgel bio-ink, a microgel scaffold containing a nerve-vascular network and a preparation method and application thereof, and belongs to the technical field of biological medicine. The microgel bio-ink prepared by the application has a significant self-healing ability when printing, ensures high-precision extrusion molding, has excellent shear thinning ability, and the viscosity is significantly reduced with the increase of the shear rate; the microgel bio-ink keeps a solid structure at a physiological temperature and has temperature-sensitive stability; the self-healing effect is significant, the mechanical stability of the printed body is improved, and the microgel bio-ink has good printing extrusion and forming ability. The application realizes the composite printing of neuron-carrying microspheres and vascular endothelial cells, successfully constructs a microgel scaffold containing a nerve-vascular network, and can be used for simulating the microenvironment interaction mechanism in vivo and providing an integrated solution for complex tissue defect repair.

The invention discloses a dual crosslinking mechanical self-adaptive biological adhesion hydrogel. The mechanical self-adaptive biological adhesion hydrogel is prepared from dopamine modified sodium alginate, bovine serum albumin and multi-arm polyethylene glycol-phthalic dicarboxaldehyde through dual covalent crosslinking. The hydrogel is endowed with the mechanical self-adaptive characteristic through the combined action of dopa and protein. Under the stress action and physiological oxidation environment, protein conformation in the hydrogel is expanded and cooperates with gradual oxidation of dopa, so that stress dissipation and adhesion strength self-enhancement along with time are realized while the material keeps flexibility, and long-term effective sealing of tissues can still be maintained under the condition of dynamic deformation. The hydrogel has the characteristics of active oxygen removal, antibiosis and enzyme response degradability, the whole system is composed of biomacromolecules and medical macromolecules, and the hydrogel has good biocompatibility and in-vivo degradability. And a new technical approach is provided for the sealed repair of airway fistula, gastrointestinal fistula and other tissue defects and the development of implantable bioadhesive materials.

To provide an improved strap having one-way locking (knotless) characteristics suitable for gathering tissue, such as closing a hernia defect.SOLUTION: The device 1 includes a locking head 5 at a proximal end, a strap 3 extending from the locking head 5, and a leader 7 extending from the strap 3. The leader 7 may have a loop 9 or other protuberance at its distal end to facilitate grasping during a surgical procedure, or alternatively, the leader 7 may have a distal end without a grasping feature in some embodiments, as it may be easily grasped by some surgical tools even without an engagement feature at the end. In general, leader 7 may be grasped anywhere along its length by any common tool used to grasp sutures.SELECTED DRAWING: Figure 1

The invention provides a material omics strategy combining bioinformatics and mathematical modeling. The material omics strategy is used for regulating extracellular matrix-based hydrogel. The prepared improved material can be particularly used for treating tissue defect diseases such as tendon defects. The hydrogel can be directly used as a transplantation material and other treatment means, and the provided hydrogel can also be indirectly applied to preparation of differentiated stem cells, cell-derived extracellular matrix products or extracellular vesicles. In addition, the invention also provides a preparation method of the hydrogel, the transplantation material, the differentiated stem cell, the extracellular matrix and the vesicle product, and a method for repairing tissue defects by using the materials.

A tissue recruiting device for treating and closing a defect and methods of using the same. The tissue recruiting device includes a tissue recruiting assembly with two grasping devices and a shroud. An actuation assembly couples control actuators to the grasping devices via actuation elements. The grasping devices may be maneuvered to grasp tissue from different locations by actuation of the control actuators. The grasping devices may be retracted into a shroud to substantially close the defect. The grasping devices and the shroud may be decoupled from the remainder of the tissue recruiting assembly to maintain closure of the defect.

Disclosed are methods of lyophilizing a tissue sample comprising obtaining a tissue sample, contacting the tissue sample with a lyoprotectant solution, freezing the tissue sample, performing a first drying step of the tissue sample after freezing, and performing a second drying step of the tissue sample after the first drying step. Disclosed are lyophilized tissues prepared using the disclosed methods of lyophilizing a tissue sample comprising obtaining a tissue sample, contacting the tissue sample with a lyoprotectant solution, freezing the tissue sample, performing a first drying step of the tissue sample after freezing, and performing a second drying step of the tissue sample after the first drying step. Disclosed are methods of treating a wound or tissue defect comprising administering a reconstituted lyophilized tissue to the wound or tissue defect.

A coiled needle and deployment methods of the same are disclosed herein. The coiled needle includes a coiled body with one or more complete turns and at least one sharp end. The coiled needle may be used to close a patent foramen ovale (PFO) by advancing the coiled needle to a location adjacent to the PFO, positioning a first end of the coiled needle against tissue adjacent to the PFO, puncturing the tissue with the first end of the coiled needle, and rotating the coiled needle to advance the coiled needle through tissue of both a septum primum and a septum secundum defining the PFO. PFO closure systems and suture delivery systems including such coiled needles are further described.

The invention provides recombinant humanized VII type collagen as well as a production method and application thereof, and belongs to the technical field of genetic engineering. The recombinant VII type humanized collagen has excellent cell adhesion activity and good biocompatibility, can be used for tissue defect repair, and does not generate immunological rejection and anaphylactic reaction when being applied to a human body.

A medical device for approximating and securing tissue without requiring knots includes a lock-head, a strap section, distal protuberance, and a leader section. The device also includes a transition section between the leader and the strap section and a stiffening section proximal to the protuberance. The leader section is used to draw the strap section into the body through small apertures in tissue and the transition section provides a gradual transition in stiffness and size between the leader and the strap section.

A medical device for approximating and securing tissue without requiring knots includes a lock-head, a strap section, distal protuberance, and a leader section. The device also includes a transition section between the leader and the strap section and a stiffening section proximal to the protuberance. The leader section is used to draw the strap section into the body through small apertures in tissue and the transition section provides a gradual transition in stiffness and size between the leader and the strap section.

The application discloses a composite hydrosol and a preparation method and application thereof, and belongs to the technical field of biological materials. The composite hydrosol is obtained by heating and dissolving a mixed dispersion liquid containing lipoic acid and lipoic acid salt and cooling, and has good biocompatibility, biodegradability and anti-inflammatory activity. The composite hydrosol can be used as a tissue adhesive for adhesion of defects of various tissues and can be used as a tissue filler for repairing irregular tissue defects. In addition, the composite hydrosol can be used as a carrier material to load various bioactive components and has application prospects in drug delivery and the like.

The application relates to the field of medical devices, in particular to a closure device with a pre-positioning mechanism, which comprises a delivery catheter, an implant for closing a tissue defect, and a puncture mechanism and a positioning mechanism arranged in the delivery catheter, the positioning mechanism comprising a connecting mechanism, a support member arranged at the distal end of the connecting mechanism, and a pre-positioning mechanism arranged on the support member, the pre-positioning mechanism being located in the left atrium and hanging on the secondary septal tissue after the support member reaches a predetermined position, and the support member can provide a support force for the puncture mechanism when the puncture mechanism reciprocally punctures the tissue to be closed; in the application, the support member can provide bidirectional support for the puncture mechanism when the puncture mechanism reciprocally punctures the tissue, the puncture precision is improved, the tissue can be prevented from being damaged, the pre-positioning mechanism can prevent the positioning mechanism from deviating, and the application has good clinical significance.

The invention discloses a preparation method and application of prostate lineage progenitor cells obtained by multi-stage induction of human induced pluripotent stem cells. The method comprises the following steps: sequentially inducing to form a definitive endoderm, a posterior intestinal urogenital sinus progenitor cell and a prostate progenitor cell, and at the third stage, adopting a collaborative regulation system in which LDN193189 and CHIR99021 are simultaneously added, so that the lineage specialization efficiency and the cell amplification capacity are remarkably improved. And further sorting through a CD49f high-expression membrane marker combination, a Trop2 positive membrane marker combination, a CD26 low-expression membrane marker combination and a CD117 negative membrane marker combination, so that a cell population with high purity, low heterogeneity and immunoregulation potential can be obtained. The cell can reconstruct a gland structure and secrete prostate specific protein in an animal model, and meanwhile, the inflammatory microenvironment is improved through immune tolerance related pathways. The process can realize batch production and maintain product activity and stability, and is suitable for treating chronic prostatitis, postoperative tissue defect, dysfunction and other diseases.

The application provides a digital pathology image enhancement method and system based on HSV-RGB combined disturbance and rough random occlusion, solves problems such as digital pathology image analysis, and comprises the following steps: S1, color disturbance; S2, tissue defect simulation; and S3, sample output. The application has the advantages of good compatibility, high flexibility and the like.

The invention relates to the technical field of biological scaffolds, in particular to a silk fibroin 4D intelligent hydrogel biological scaffold and a preparation method thereof.The silk fibroin 4D intelligent hydrogel biological scaffold is prepared by taking silk fibroin as a main component and combining with genipin as a cross-linking agent through a printing technology, and the genipin and the silk fibroin form a temperature-sensitive physical cross-linked network; the prepared biological scaffold has the 4D intelligent characteristic and is cured into a temporary shape on a low-temperature platform, and when the hydrogel biological scaffold is implanted into a body (37 DEG C), under stimulation of body temperature, the hydrogel biological scaffold can spontaneously change so as to adapt to and be tightly attached to a specific tissue defect part, so that the tissue defect part is prevented from being damaged. The technical problem that the matching degree of a traditional static stent and a defect part is poor is solved, personalized customization can be performed on damaged tissues of different patients, and the stent has good biocompatibility, excellent mechanical strength, biodegradability and intelligent response characteristics, can promote cell adhesion, proliferation and tissue regeneration, and is particularly suitable for soft tissue repair.

The invention belongs to the field of stem cell genetic engineering, and provides application of NLRP12 in anti-inflammatory osteogenesis promotion of periodontal ligament stem cells. According to the NLRP12 overexpression PDLSCs, the anti-inflammatory gene is applied to periodontal regeneration treatment, and the anti-inflammatory capacity and the tissue defect repairing capacity after the PDLSCs are transplanted into a body are effectively improved. An exogenous gene is transferred into PDLSCs through a virus vector, stable transfection and efficient and continuous expression of an anti-inflammatory gene are achieved, and the repair effect of periodontitis tissue defects is promoted. In-vitro experiments show that NLRP12 overexpression in an inflammatory microenvironment alleviates the inflammatory response and osteogenic differentiation inhibition of PDLSCs, which shows that the expression of proinflammatory factors IL-6 and IL-8 is down-regulated, the expression levels of anti-inflammatory factors IL-10 and osteogenic related proteins RUNX2, COL1 and BMP2 are up-regulated, ALP dyeing and alizarin red dyeing are deepened, and mineralized nodule formation is increased. The NLRP12 overexpression PDLSCs is applied to a rat experimental periodontitis model in vivo, inflammatory reaction is remarkably relieved, and periodontal tissue regeneration of periodontitis rats is promoted. Mechanism research shows that the NLRP12 alleviates the inflammatory response of PDLSCs and osteogenic differentiation inhibition by inhibiting an NF-kappa B pathway. The NLRP12 overexpression PDLSCs are suitable for repairing periodontal tissue defects, and have a good clinical application prospect.

A system containing an electroceutical patch (ePATCH) in combination with a catheter having retractable electrodes (CATRE) which can be used to treat tissue defects, such for the repair of lumen defects, is described herein. The system was shown to be able to seal lumen defects on both synthetic and wet tissue substrates.