300 results about "Connective tissue fiber" patented technology

The invention discloses a preparation method and application of a cell culture liver based on a microcarrier. The method comprises the steps of obtaining liver seed cells, preparing the microcarrier, culturing cells in vitro based on the microcarrier, preparing the cell culture liver and the like. The preparation method of the cell culture liver based on the microcarrier is provided for the first time, and the preparation scheme that a cell-microcarrier compound serves as a structural unit and is assembled into the cell culture liver after being mixed with an edible material is adopted, so that on one hand, simulation of a real liver lobule structure can be achieved, and on the other hand, simulation of the liver lobule structure can be achieved; the whole structure relation of liver lobules and connective tissues in liver tissues can be simulated, full reduction of animal liver appearance and structural characteristics is achieved, edible materials used in the preparation process are low in price and good in biocompatibility, and the preparation method is suitable for large-scale industrial production. Liver seed cells can be subjected to efficient amplification and albumin synthesis through three-dimensional culture on a microcarrier prepared on the basis of edible materials, so that the cell culture liver is close to a real animal liver in the aspect of nutrition.

The invention relates to the technical field of medical biological materials, in particular to an aquatic animal source acellular matrix and a preparation method and application thereof. The aquatic animal source acellular matrix is prepared by performing pretreatment and acellular treatment on connective tissues of aquatic animals; the pretreatment method comprises the following steps: carrying out enzymolysis for 12-60 hours by using biological enzyme at the temperature of 0-15 DEG C, and then removing muscular tissues, basilar membranes and epidermal layers to obtain the miscellaneous tissue-free dermal tissues. The aquatic animal-derived acellular matrix provided by the invention has a bionic structure similar to or consistent with animal original tissues, does not contain components possibly causing immune risks, and is high in clinical safety and effectiveness.

The invention relates to the technical field of elastin peptides, in particular to a method for extracting elastin peptides from animal cartilage, which comprises the following specific steps: removing surface connective tissues and fat from animal cartilage, cleaning with normal saline, grinding and degreasing to obtain pretreated cartilage particles; putting the pretreated cartilage particles into a container, adding ionic liquid and stirring; after stirring is completed, centrifugally collecting precipitates; adding the precipitate into a phosphate buffer solution to form a suspension; adding an immobilized compound enzyme, and carrying out an enzymolysis reaction; after the enzymolysis is completed, raising the temperature for inactivation; filtering to obtain enzymatic hydrolysate; adding macroporous resin into the enzymatic hydrolysate, and filtering to obtain clear liquid; and carrying out ultrafiltration on the clear liquid to obtain the elastin peptide. The ionic liquid interacts with impurities in the animal cartilage, so that the impurities are easier to remove, a purer raw material environment is created for subsequent elastin peptide extraction, and the extraction efficiency is improved.

The present invention relates to a part (2) intended to be used in association with an implant (4) which is itself intended to be secured in a jaw, this part comprising, in series from its distal end (20) towards its proximal end (21), a distal portion (22) for securing in the implant, an intermediate portion (23) for attachment to the epithelial and / or connective tissue, and a proximal portion (24), this part also comprising at least two zones whose surfaces have a different structure, the structure of the surface of at least one of these two zones, located in said intermediate portion (23), said first zone being ordered and repetitive, adapted to the growth of the epithelial tissue and / or the connective tissue and having engravings forming corrugations with a depth of less than 0.5 microns.

According to the scheme, the expanded polytetrafluoroethylene artificial blood vessel with the anticoagulant coating and preparation and application are provided, firstly, groups such as carboxyl and amino are formed on the inner surface of the expanded polytetrafluoroethylene artificial blood vessel through co-auto-polymerization reaction of L-dopamine and polyethyleneimine in a Tris aqueous alkali, then epoxy chloropropane serves as a cross-linking agent, and the expanded polytetrafluoroethylene artificial blood vessel with the anticoagulant coating is prepared. Trihydroxymethyl methylamino propanesulfonic acid is cross-linked to the inner surface of the membrane, carboxyl and sulfonic acid groups on the surface of the membrane endow the expanded polytetrafluoroethylene artificial blood vessel with anticoagulation performance, meanwhile, the hydrophobic performance of the outer surface is reserved, anticoagulation finishing is conducted on the inner wall of the expanded polytetrafluoroethylene artificial blood vessel, and the hydrophobic performance of the outer wall of the blood vessel is reserved; the expanded polytetrafluoroethylene artificial blood vessel has an anticoagulation function and does not influence the growth of fiber cells and fiber connective tissues on the surface of the blood vessel.

Devices and methods for tissue treatment produce a mechanical stimulation therapy and electromagnetic field therapy. The mechanical stimulation therapy provides stimulation of blood circulation and stimulates treated cells. The electromagnetic field enables thermal treatment of tissue. Combination of both therapies improves soft tissue treatment, mainly connective tissue in the skin area and fat reduction.

Various processes, algorithms, and systems are provided herein for assisting physicians in distinguishing among related diseases, such as distinguishing connective tissue associated interstitial lung disease from idiopathic pulmonary fibrosis. Methods for generating such processes, algorithms, and systems are also disclosed. In some embodiments, a preliminary diagnosis of a set of possible diseases is obtained, along with protein count information from a patient's blood sample. Additional, patient-specific information (e.g., age, sex, etc.) may also be obtained. The data is processed by a trained machine learning algorithm, to output a differential diagnosis of which of the set of possible diseases is present for that patient. Based on the diagnosis, a treatment course can be selected, and further information can be tracked regarding the patient's outcome.

The invention discloses composite hydrogel bio-ink with a function of space-time delivery of double growth factors as well as a preparation method and application of the composite hydrogel bio-ink, and belongs to the technical field of biomedical materials and tissue engineering. According to the preparation method, imidazolyl-modified methacrylated gelatin and double-bonded PF127 are compounded, and meanwhile, free vascular endothelial growth factors and connective tissue growth factor-loaded PLGA microspheres are entrapped in a system, so that the bio-ink with excellent printability, high adhesion, swelling resistance and space-time delivery function is formed; the bio-ink physically has the advantages of high printing fidelity, low swelling property, high mechanical strength and strong tissue adhesion of a synthetic material; in biology, the system can be used as an intelligent carrier to realize programmed space-time delivery of various growth factors, so that complex cell biological behaviors are accurately arranged, and real regeneration of functional tissues is guided.

The invention relates to the technical field of cell separation and culture, in particular to a method for separating and culturing haematochrome cells of plectropomus leopardus. The separation method of the plectropomus leopardus haematochrome cells is created for the first time, the EDTA digestive juice is used for pre-digesting epidermis cells on the outer layers of the plectropomus leopardus fins, then the collagenase digestive juice is used for digesting connective tissues (pigmented cells) of internal dermis, digestive enzymes such as trypsin and other mechanical methods which have great damage to the cells are not used, and the method is suitable for large-scale production of the plectropomus leopardus haematochrome cells. The plectropomus leopardus haematochrome cell can reduce cell damage, can be effectively separated to obtain the plectropomus leopardus haematochrome cell which is high in purity, good in activity and capable of being cultured in vitro, and lays a foundation for subsequent experiments such as single cell sequencing, cell in-vitro culture and molecular function verification.

The invention discloses a recombinant I-type collagen, a preparation method and application, and belongs to the technical field of binding tissue peptides, the recombinant I-type collagen is formed by sequentially connecting the following modules in series from the N terminal to the C terminal: a signal peptide, a truncated I-type collagen and a His tag; the nucleotide sequence of the truncated I type collagen is as shown in any one of SEQ ID NO.3, SEQ ID NO.4, SEQ ID NO.5 and SEQ ID NO.6; the nucleotide sequence of the truncated I type collagen is as shown in any one of SEQ ID NO.3, SEQ ID NO.4, SEQ ID NO.5 and SEQ ID NO.6; according to the invention, a mammalian expression system and a 293T cell line are used for expressing recombinant I-type collagen, so that collagen with natural biological activity is obtained, and the proliferation efficiency and repair capacity of the collagen for promoting HSF cells can be improved.

The invention discloses a method for improving the texture characteristics of canned abalone. After fresh abalone is taken out, cleaned, water-controlled, shaped and salt-ground, composite protease is used to decompose collagen and elastin in the abalone meat and hydrolyze connective tissue fibers. At the same time, ultrasonic treatment is used to destroy the original muscle structure, which effectively reduces the hardness value of the abalone meat. After the treatment, the abalone meat is cleaned, water-controlled, blanched, tooth-removed and graded, and then placed in a 500V DENBA+ electrostatic wave preservation system and treated at 4°C for 1h. After that, the abalone meat is subjected to metal detection, weighing and canning, vacuum sealing, can washing, sterilization and cooling, and drying. The canned abalone treated by the above steps can improve its rubber meat texture characteristics to the greatest extent, effectively ensure the moisture of the abalone meat, and maintain its nutritional components, good flavor and taste.

The invention relates to the technical field of preparation of stimuli-responsive hydrogel, in particular to sea cucumber ion skin imitating double-dynamic topology hydrogel as well as a preparation method and application of the sea cucumber ion skin imitating double-dynamic topology hydrogel. The thermo-sensitive poly (N-isopropylacrylamide) hydrogel is used as a continuous matrix and is used for simulating the dynamic adaptability of sea cucumber skin; and the ionic conductive microgel is used as a rigid dispersion phase and is used for simulating a collagenous fiber network of a connective tissue layer of the sea cucumber. The microgel is dispersed in the poly (N-isopropylacrylamide) hydrogel and is physically entangled with the poly (N-isopropylacrylamide) hydrogel to form a cross-linking point, so that a dynamic network of'rigid node-flexible matrix 'is constructed. The dynamic network structure endows the double-dynamic topological hydrogel with force and heat double toughening properties and ultralow mechanical hysteresis, so that the double-dynamic topological hydrogel has excellent elasticity, fatigue resistance, low hysteresis and strain rate insensitivity, and further shows wide application prospects in the fields of flexible sensing and wearable equipment.

[Problem] To provide an additional use of a polysaccharide derivative such as a tissue adhesive material[Solution] According to some embodiments, a tissue adhesive material is provided, which is intended to be used for a hard and thick tissue and / or a connective tissue and comprises a polysaccharide derivative having such a structure that a group represented by formula (A) is introduced in an acidic, basic or amphoteric polysaccharide. According to some embodiments, a crosslinked structure and a tissue adhesive material comprising the crosslinked structure are provided, in which the crosslinked structure comprises (a) a polysaccharide derivative having such a structure that a group represented by formula (A) is introduced into an acidic, basic or amphoteric polysaccharide and (b) at least one of an amino-group-containing polymer and an amino-group-containing low-molecular-weight compound each containing at least two primary amino groups, hydrazide groups or aminooxy groups and a group represented by formula (x1), and the crosslinking is achieved by the covalent bonding between a primary amino group, a hydrazide group or an aminooxy group contained in the amino-group-containing polymer and the amino-group-containing low-molecular-weight compound and the group represented by formula (A) contained in the polysaccharide derivative through a Schiff base. According to some embodiments, a tissue adhesive material having a gel-like form is provided, which comprises a polysaccharide derivative having such a structure that a group represented by formula (A-2) is introduced into a polysaccharide containing an amino group, in which the group represented by formula (A-2) is introduced into the polysaccharide by substituting for a hydrogen atom in the amino group in the polysaccharide to form an amide bond.

The present disclosure provides a method and a kit for inducing growth or regeneration of a damaged connective tissue of a subject, or the treatment thereof by applying a clot of blood that is withdrawn from the subject. The connective tissue can be tendon. By one implementation of the present invention, the blood that is withdrawn from the subject is introduced to a lumen of a connective tissue enveloping hollowed element that envelopes the damaged portion of the connective tissue while controllably coagulating it within the lumen to form a clot on the damaged portion of the connective tissue. e.g. by mixing it with coagulation initiators shortly prior to the introduction of the blood into the lumen. The clot, while in physical contact with the damaged portion of the connective tissue, enhances the rehabilitation of the connective tissue and may partially or fully restore its functionality.

Provided herein are compositions and methods of use for using exosomes derived from mesenchymal stem cells cultured on a scaffold consisting of synthetic fibers. The compositions are useful for treating musculoskeletal or connective tissue repair or degenerative conditions, for example, tendinopathy and tendon injury, and pain.

The use of a divalent metal lactate in products for promoting tissue regeneration, growth, healing and damage repair, belonging to the technical field of biological medicines. Preferably, the divalent metal lactate comprises one or a combination of calcium lactate, magnesium lactate, zinc lactate and ferrous lactate. Provided is a new manner of treatment for tissue damage and repair, and prevention and treatment of tissue fibrosis and / or scar tissue generation, said manner of treatment having wide prospects of application in terms of damage treatment and repair of tissue such as skin, connective tissue, tendons and fascia.

The invention belongs to the technical field of biological medicines, and particularly relates to application of divalent metal lactate in a product for promoting tissue regeneration, growth, healing and damage repair. Preferably, the divalent metal lactate comprises one or a combination of more of calcium lactate, magnesium lactate, zinc lactate and ferrous lactate. The invention provides a new treatment mode for tissue injury and repair, and prevention and treatment of tissue fibrosis and / or scar tissue generation, and has wide application prospects in the aspects of injury treatment and repair of tissues such as skin, connective tissues, tendons, fascia and the like.

The invention discloses an application of a meroterpenoid compound I in preparation of an anti-heart failure medicine. The meroterpenoid I separated and purified from marine fungi Aspergillus terreus GZU-311 is used for treating a mouse heart failure model induced by adriamycin or coronary artery left anterior descending branch ligation, and it is found that the meroterpenoid I can improve the heart function of a mouse, reduce myocardial cell damage, inhibit myocardial connective tissue thickening and improve myocardial cell arrangement disorder, and the meroterpenoid I can be used for treating the heart failure of the mouse induced by adriamycin or coronary artery left anterior descending branch ligation, so that the meroterpenoid I can be used for treating the heart failure of the mouse induced by adriamycin or coronary artery left anterior descending branch ligation. Inflammatory cell infiltration is reduced, and the effect of resisting heart failure is achieved. Therefore, the invention provides the application of the meroterpenoid I in preparing the heart failure resisting medicine. According to the invention, medicine source molecules for treating heart failure are enriched, and development and clinical treatment of anti-heart-failure medicines are facilitated.

The invention relates to the technical field of medical information processing, and particularly discloses a method for identifying a recurrent laryngeal nerve and an adjacent anatomical structure thereof in an ultrasonic image, and the method comprises the steps: obtaining a two-dimensional thyroid region ultrasonic image, and carrying out the fine marking of the recurrent laryngeal nerve, the true thyroid envelope, the false thyroid envelope and the connective tissue; performing uniform size adjustment, normalization and data enhancement on the marked image; performing semantic segmentation on the image to obtain a segmented image containing the recurrent laryngeal nerve and an adjacent anatomical structure region; extracting depth features of the local structure of the segmented image; extracting high-dimensional geometric structure features of the segmented image; fusing the extracted features to form a comprehensive feature vector; and inputting the fused comprehensive feature vector into a classification module to perform automatic classification and identification of the recurrent laryngeal nerve and the adjacent structure thereof. According to the invention, the recognition accuracy and stability in the ultrasonic image can be improved.

Provided is an “all natural” biologic-enhancing beverage (BioBeverage), which contains, in addition to purified water, electrolytes, vitamins, anti-oxidants, micronutrients, natural fruit infusion, natural sweetener to improve taste, enhance GI Biome viability, provide antimicrobial benefits and reduce oxidative stress, Citicoline, to enhance brain / neurologic function, neurologic maintenance, repair and regeneration, CoQ10, to enhance cardiovascular energy and health, Hydroxyproline, to enhance connective tissue maintenance, repair and regeneration, and a niacinamide adenine dinucleotide (NAD+) precursor to promote overall health, including neurologic, cardiovascular and connective tissues maintenance and repair, and if present, reduce risks and symptoms of multiple neurodegenerative disorders by maintenance of healthy mitochondrial function.

To provide a compound and the use thereof for the treatment for regeneration of connective tissue following the degeneration process of articular cartilage due to osteoarthritis, in the case of humans, the degeneration process of articular cartilage caused by excessive stress, for the treatment of skin aging, and for the prevention of skin cancer.SOLUTION: For example, an amino acid derivative of glucosamine having the following structure is provided.SELECTED DRAWING: None

Embodiments of the present invention disclose a genetically modified cell that accelerates tissue repair by overexpressing CXCR4. This motile cell can be administered to subjects in need, enhancing tissue regeneration compared to unmodified cells. The cell may include various progenitor cell lines and demonstrates low NF-Kb pathway activity, which suppresses catabolic activity and promotes anabolism in inflammatory environments. The invention also encompasses methods of treatment using these cells, compositions containing them, and kits for their delivery.

An orally administered composition for treating or reducing damage to connective tissue or for treating or reducing inflammatory symptoms associated with damage to connective tissue includes a synergistic combination of (i) avocado / soybean unsaponifiables; and (ii) a lipoic acid or a salt or derivative thereof. A method for treating or reducing damage to connective tissue, for treating or reducing inflammatory symptoms associated with damage to connective tissue, or for reducing the level of one or more inflammatory mediators in connective tissue includes administering the orally administered composition to an avian or mammalian subject.

The present invention relates to a dental / medical device consisting of an absorbable synthetic membrane, preferably in the color of white to gray, comprising poly (dioxanone) and optionally graphene and stem cells for guided tissue reconstruction and / or regeneration of bone defects and other connective tissues, such as gingiva, skin, cartilage or cornea. Furthermore, the invention relates to the use of said device for the above related applications and to a method for producing said device.

The present disclosure relates to compositions and methods for treating vascular Ehlers Danlos Syndrome and associated connective tissue disorders.

The present disclosure relates to methods of treating, preventing, or modulating diabetes in a patient in need thereof using a device for transplanting cells into a host body, specifically a method of treating diabetes in a patient in need thereof, comprising: implanting a device in the patient, wherein the device comprises: a porous scaffold comprising an immunologically compatible polymer mesh forming the walls of at least one chamber, an opening at either or both of a proximal end and a distal end of the chamber, at least one removable, non-porous plug configured to be positioned within the lumen of the at least one chamber, maintaining the device in the patient's body until the device is infiltrated with vascular and connective tissues; and infusing the chamber with cells, wherein at least some of the cells express insulin

The invention relates to a multifunctional collagen-based edible food packaging film with pH responsiveness and preparation, and belongs to the field of biodegradable packaging materials. The method comprises the following steps: swelling collagen, wherein the collagen is obtained by extracting animal tendons or connective tissues or animal skins; mixing the turbid liquid with a grape skin red pigment with pH responsiveness to obtain a collagen mixed solution; and adding the ganoderma lucidum hypha liquid into the collagen mixed liquid, pouring into a mold, and drying to obtain the pH-responsive multifunctional collagen-based food packaging film. The food film designed by the invention has pH responsiveness, the film can be changed from pink to yellow along with the change of environment pH from acidity to alkalinity, and the preservation and dynamic monitoring of food materials are realized; the film materials are all biodegradable materials, are green and environment-friendly, are simple in process, and have the advantages of high mechanical strength, excellent oxidation resistance and antibacterial property, greenness, environment friendliness and no toxicity.

Disclosed is a composition for treating a joint disease or a connective tissue disease, a composition for cartilage regeneration, or a composition for treating an inflammatory disease, each of the compositions containing dextran, poloxamer or a mixture thereof. The composition stays in the joint or connective tissue for a long time due to the shock-absorbing effect, coating effect or anti-inflammation effect, relieves the shock, covers a damaged portion in a specific manner thereto, or reduces inflammation of an adhered portion. Thus, the composition may be useful for the treatment of the joint disease, the connective tissue disease, or for the cartilage regeneration.

A device for attenuating attachment of acicular bones to muscles or adipose tissue in fillets. An electrode coupled to the power supply and in electrical contact with the first end of the needle bone conducts current to the needle bone. The needle bone conducts current from a first end of the needle bone to a second end of the needle bone. The acicular bone has a current resistivity that results in Joule heating that produces sufficient heat in connective tissue that attaches the acicular bone to muscle or adipose tissue in the fillet to degrade or denature the connective tissue and thereby attenuate attachment of the acicular bone to the muscle or adipose tissue.

An orally administrable composition for treating or reducing damage to connective tissue or for treating or reducing inflammatory symptoms associated with damage to connective tissue includes a synergistic combination of: (i) avocado / soybean unsaponifiables; and (ii) lipoic acid, or a salt or derivative thereof. Methods for treating or reducing damage to connective tissue, for treating or reducing inflammatory symptoms associated with damage to connective tissue, or for reducing levels of one or more inflammatory mediators in connective tissue include administering the orally administrable composition to an avian or mammalian subject.