173 results about "Connective tissue fiber" patented technology

The invention discloses a preparation method and application of a cell culture liver based on a microcarrier. The method comprises the steps of obtaining liver seed cells, preparing the microcarrier, culturing cells in vitro based on the microcarrier, preparing the cell culture liver and the like. The preparation method of the cell culture liver based on the microcarrier is provided for the first time, and the preparation scheme that a cell-microcarrier compound serves as a structural unit and is assembled into the cell culture liver after being mixed with an edible material is adopted, so that on one hand, simulation of a real liver lobule structure can be achieved, and on the other hand, simulation of the liver lobule structure can be achieved; the whole structure relation of liver lobules and connective tissues in liver tissues can be simulated, full reduction of animal liver appearance and structural characteristics is achieved, edible materials used in the preparation process are low in price and good in biocompatibility, and the preparation method is suitable for large-scale industrial production. Liver seed cells can be subjected to efficient amplification and albumin synthesis through three-dimensional culture on a microcarrier prepared on the basis of edible materials, so that the cell culture liver is close to a real animal liver in the aspect of nutrition.

The present invention relates to a part (2) intended to be used in association with an implant (4) which is itself intended to be secured in a jaw, this part comprising, in series from its distal end (20) towards its proximal end (21), a distal portion (22) for securing in the implant, an intermediate portion (23) for attachment to the epithelial and / or connective tissue, and a proximal portion (24), this part also comprising at least two zones whose surfaces have a different structure, the structure of the surface of at least one of these two zones, located in said intermediate portion (23), said first zone being ordered and repetitive, adapted to the growth of the epithelial tissue and / or the connective tissue and having engravings forming corrugations with a depth of less than 0.5 microns.

According to the scheme, the expanded polytetrafluoroethylene artificial blood vessel with the anticoagulant coating and preparation and application are provided, firstly, groups such as carboxyl and amino are formed on the inner surface of the expanded polytetrafluoroethylene artificial blood vessel through co-auto-polymerization reaction of L-dopamine and polyethyleneimine in a Tris aqueous alkali, then epoxy chloropropane serves as a cross-linking agent, and the expanded polytetrafluoroethylene artificial blood vessel with the anticoagulant coating is prepared. Trihydroxymethyl methylamino propanesulfonic acid is cross-linked to the inner surface of the membrane, carboxyl and sulfonic acid groups on the surface of the membrane endow the expanded polytetrafluoroethylene artificial blood vessel with anticoagulation performance, meanwhile, the hydrophobic performance of the outer surface is reserved, anticoagulation finishing is conducted on the inner wall of the expanded polytetrafluoroethylene artificial blood vessel, and the hydrophobic performance of the outer wall of the blood vessel is reserved; the expanded polytetrafluoroethylene artificial blood vessel has an anticoagulation function and does not influence the growth of fiber cells and fiber connective tissues on the surface of the blood vessel.

Various processes, algorithms, and systems are provided herein for assisting physicians in distinguishing among related diseases, such as distinguishing connective tissue associated interstitial lung disease from idiopathic pulmonary fibrosis. Methods for generating such processes, algorithms, and systems are also disclosed. In some embodiments, a preliminary diagnosis of a set of possible diseases is obtained, along with protein count information from a patient's blood sample. Additional, patient-specific information (e.g., age, sex, etc.) may also be obtained. The data is processed by a trained machine learning algorithm, to output a differential diagnosis of which of the set of possible diseases is present for that patient. Based on the diagnosis, a treatment course can be selected, and further information can be tracked regarding the patient's outcome.

The invention discloses composite hydrogel bio-ink with a function of space-time delivery of double growth factors as well as a preparation method and application of the composite hydrogel bio-ink, and belongs to the technical field of biomedical materials and tissue engineering. According to the preparation method, imidazolyl-modified methacrylated gelatin and double-bonded PF127 are compounded, and meanwhile, free vascular endothelial growth factors and connective tissue growth factor-loaded PLGA microspheres are entrapped in a system, so that the bio-ink with excellent printability, high adhesion, swelling resistance and space-time delivery function is formed; the bio-ink physically has the advantages of high printing fidelity, low swelling property, high mechanical strength and strong tissue adhesion of a synthetic material; in biology, the system can be used as an intelligent carrier to realize programmed space-time delivery of various growth factors, so that complex cell biological behaviors are accurately arranged, and real regeneration of functional tissues is guided.

The invention discloses a recombinant I-type collagen, a preparation method and application, and belongs to the technical field of binding tissue peptides, the recombinant I-type collagen is formed by sequentially connecting the following modules in series from the N terminal to the C terminal: a signal peptide, a truncated I-type collagen and a His tag; the nucleotide sequence of the truncated I type collagen is as shown in any one of SEQ ID NO.3, SEQ ID NO.4, SEQ ID NO.5 and SEQ ID NO.6; the nucleotide sequence of the truncated I type collagen is as shown in any one of SEQ ID NO.3, SEQ ID NO.4, SEQ ID NO.5 and SEQ ID NO.6; according to the invention, a mammalian expression system and a 293T cell line are used for expressing recombinant I-type collagen, so that collagen with natural biological activity is obtained, and the proliferation efficiency and repair capacity of the collagen for promoting HSF cells can be improved.

The invention relates to the technical field of preparation of stimuli-responsive hydrogel, in particular to sea cucumber ion skin imitating double-dynamic topology hydrogel as well as a preparation method and application of the sea cucumber ion skin imitating double-dynamic topology hydrogel. The thermo-sensitive poly (N-isopropylacrylamide) hydrogel is used as a continuous matrix and is used for simulating the dynamic adaptability of sea cucumber skin; and the ionic conductive microgel is used as a rigid dispersion phase and is used for simulating a collagenous fiber network of a connective tissue layer of the sea cucumber. The microgel is dispersed in the poly (N-isopropylacrylamide) hydrogel and is physically entangled with the poly (N-isopropylacrylamide) hydrogel to form a cross-linking point, so that a dynamic network of'rigid node-flexible matrix 'is constructed. The dynamic network structure endows the double-dynamic topological hydrogel with force and heat double toughening properties and ultralow mechanical hysteresis, so that the double-dynamic topological hydrogel has excellent elasticity, fatigue resistance, low hysteresis and strain rate insensitivity, and further shows wide application prospects in the fields of flexible sensing and wearable equipment.

The invention relates to the technical field of medical information processing, and particularly discloses a method for identifying a recurrent laryngeal nerve and an adjacent anatomical structure thereof in an ultrasonic image, and the method comprises the steps: obtaining a two-dimensional thyroid region ultrasonic image, and carrying out the fine marking of the recurrent laryngeal nerve, the true thyroid envelope, the false thyroid envelope and the connective tissue; performing uniform size adjustment, normalization and data enhancement on the marked image; performing semantic segmentation on the image to obtain a segmented image containing the recurrent laryngeal nerve and an adjacent anatomical structure region; extracting depth features of the local structure of the segmented image; extracting high-dimensional geometric structure features of the segmented image; fusing the extracted features to form a comprehensive feature vector; and inputting the fused comprehensive feature vector into a classification module to perform automatic classification and identification of the recurrent laryngeal nerve and the adjacent structure thereof. According to the invention, the recognition accuracy and stability in the ultrasonic image can be improved.

To provide a compound and the use thereof for the treatment for regeneration of connective tissue following the degeneration process of articular cartilage due to osteoarthritis, in the case of humans, the degeneration process of articular cartilage caused by excessive stress, for the treatment of skin aging, and for the prevention of skin cancer.SOLUTION: For example, an amino acid derivative of glucosamine having the following structure is provided.SELECTED DRAWING: None

Embodiments of the present invention disclose a genetically modified cell that accelerates tissue repair by overexpressing CXCR4. This motile cell can be administered to subjects in need, enhancing tissue regeneration compared to unmodified cells. The cell may include various progenitor cell lines and demonstrates low NF-Kb pathway activity, which suppresses catabolic activity and promotes anabolism in inflammatory environments. The invention also encompasses methods of treatment using these cells, compositions containing them, and kits for their delivery.

An orally administered composition for treating or reducing damage to connective tissue or for treating or reducing inflammatory symptoms associated with damage to connective tissue includes a synergistic combination of (i) avocado / soybean unsaponifiables; and (ii) a lipoic acid or a salt or derivative thereof. A method for treating or reducing damage to connective tissue, for treating or reducing inflammatory symptoms associated with damage to connective tissue, or for reducing the level of one or more inflammatory mediators in connective tissue includes administering the orally administered composition to an avian or mammalian subject.

The present disclosure relates to methods of treating, preventing, or modulating diabetes in a patient in need thereof using a device for transplanting cells into a host body, specifically a method of treating diabetes in a patient in need thereof, comprising: implanting a device in the patient, wherein the device comprises: a porous scaffold comprising an immunologically compatible polymer mesh forming the walls of at least one chamber, an opening at either or both of a proximal end and a distal end of the chamber, at least one removable, non-porous plug configured to be positioned within the lumen of the at least one chamber, maintaining the device in the patient's body until the device is infiltrated with vascular and connective tissues; and infusing the chamber with cells, wherein at least some of the cells express insulin

The invention relates to a multifunctional collagen-based edible food packaging film with pH responsiveness and preparation, and belongs to the field of biodegradable packaging materials. The method comprises the following steps: swelling collagen, wherein the collagen is obtained by extracting animal tendons or connective tissues or animal skins; mixing the turbid liquid with a grape skin red pigment with pH responsiveness to obtain a collagen mixed solution; and adding the ganoderma lucidum hypha liquid into the collagen mixed liquid, pouring into a mold, and drying to obtain the pH-responsive multifunctional collagen-based food packaging film. The food film designed by the invention has pH responsiveness, the film can be changed from pink to yellow along with the change of environment pH from acidity to alkalinity, and the preservation and dynamic monitoring of food materials are realized; the film materials are all biodegradable materials, are green and environment-friendly, are simple in process, and have the advantages of high mechanical strength, excellent oxidation resistance and antibacterial property, greenness, environment friendliness and no toxicity.

Disclosed is a composition for treating a joint disease or a connective tissue disease, a composition for cartilage regeneration, or a composition for treating an inflammatory disease, each of the compositions containing dextran, poloxamer or a mixture thereof. The composition stays in the joint or connective tissue for a long time due to the shock-absorbing effect, coating effect or anti-inflammation effect, relieves the shock, covers a damaged portion in a specific manner thereto, or reduces inflammation of an adhered portion. Thus, the composition may be useful for the treatment of the joint disease, the connective tissue disease, or for the cartilage regeneration.

[Problem] To provide a new technical means that enables manufacturing a heated meat processed product containing a meat chunk having a good texture. [Solution] Provided is a method for manufacturing a heated meat processed product containing a chicken chunk, the method comprising a step for preparing a meat material to be processed, the meat material containing a chicken chunk, and a step for heating the meat material to be processed under heating conditions predetermined to achieve the following criteria: the center temperature of the chicken chunk reaches 85°C or higher; the degree of connective tissue degradation in the chicken chunk reaches 5% or more; and the yield of the heated meat processed product from the meat material to be processed (heated meat processed product / meat material to be processed) reaches 90 mass% or more.

To provide a device for preventing periodontal disease, which aims to prevent periodontal disease by increasing the blood flow in the gums through brushing with a toothbrush, rectifying the tissue fluid produced in the gums and guiding it to the attached subepithelial connective tissue, and causing the tissue fluid to seep into the gingival sulcus through the gaps between attached epithelial cells, thereby generating a pseudo self-cleaning action of the gingival sulcus. [Solution] Using a motor for an arrow feather-shaped brush, the arrow feather-shaped brush is continuously rotated from the attached gum through the cervical area toward the gingival sulcus opening, with the product of the number of rows of brush bristles that make up the feather portion of the arrow feather-shaped brush 1 and the number of rotations per minute of the motor being in the range of 240 times per minute or less, and the rotating arrow feather-shaped brush is vibrated using a vibration motor 12.

The described system designed to promote the biological attachment of fibrous connective tissue to a bone implant features a bone implant with a scaffold defining a porous attachment surface, a suction port, and a manifold that distributes a negative pressure gradient across the attachment surface. The system also includes an extracorporeal vacuum pump and a percutaneous suction hose connecting the suction port of the implant to the vacuum pump. The implant is placed in the patient's body with connective tissue applied over the attachment surface, and the suction hose is attached and connected to the vacuum pump. The vacuum pump, operating outside the body, creates a negative pressure gradient over an extended period, promoting the growth of slow growing Sharpey's fibres for superior biological integration. A highly vascularised tissue graft, such as a pedicled muscle flap, may be applied adjacent to the avascular tendon to enhance endogenous growth factor migration.

Ultrasound emitting device for applying selective treatments to hypodermic tissue (adipose and connective tissue) in rejuvenation and / or body contouring processes, comprising an electrical pulse generator, an ultrasonic transducer (3), an electronic control unit (6) equipped with specific software that controls the frequency, voltage, and duty cycle of the pulse generator, and application means (4) for orienting and directing the acoustic field onto the area to be treated on the patient / user, wherein said transducer emits in each pulse an acoustic field with an energy density not exceeding 0.7W / cm2 and a minimum emission duration of 100ms. Preferably, the transducer emits either at a single frequency or in a frequency sweep, in a frequency range between 185 kHz and 333 kHz, and even more preferably at 244 kHz.

This invention provides an organ fibrosis-related micropeptide, OFMP, and its applications. This type of micropeptide, OFMP, is a translational product with endogenous biological activity and stable existence, encoded by an open reading frame (ORF) in a long non-coding RNA. It is the first time that the long non-coding RNA encoding the micropeptide has been found to be significantly related to organ fibrosis and can encode the micropeptide. Synthetic peptides prepared based on the aforementioned OFMP micropeptide show significant inhibitory effects on several key indicators of organ fibrosis, including type I collagen α1 chain gene, α-smooth muscle actin, transforming growth factor β1, and connective tissue growth factor. This indicates that the OFMP micropeptide, the nucleotide sequence (ORF) encoding the micropeptide, and the long non-coding RNA sequence containing the nucleotide sequence have important clinical diagnostic and therapeutic value for organ fibrosis.

In some examples, a tool for, e.g., creating a sub-sternal tunnel in a patient or other use, is described. The tool may include a handle and a tunneling shaft coupled to the handle. The tunneling shaft extends from a proximal end to a distal end, and at least a portion of the tunneling shaft extends in a curved orientation between the first end to the distal end. The distal end of the tunneling shaft includes a cutting tool having a sharp edge. The cutting tool is moveable from a recessed position in which the sharp edge of the cutting tool is recessed into the distal end of the tunneling shaft to a deployed position in which the sharp edge of the cutting tool extends beyond the distal end of the tunneling shaft in the deployed position, e.g., to cut pericardium, scar tissue, and / or connective tissue with the sharp edge.

The invention relates to the technical field of medical materials, in particular to a composition for soft tissue increment and a preparation method thereof, and the composition for soft tissue increment comprises a plasma matrix compressed material and a microvesicle concentrated solution. The composition for soft tissue increment provided by the invention comprises a plasma matrix compressed material and a microvesicle concentrated solution, the plasma matrix compressed material is rich in effective substances such as platelets and immune cells, has a compact three-dimensional fibrous protein network structure, and can provide a required scaffold for soft tissue regeneration, so that the soft tissue regeneration effect is improved. The microvesicle concentrated solution contains a large number of effective biological components such as cell factors and signal molecules, regeneration of local soft tissue can be further regulated and controlled, the microvesicle concentrated solution and the microvesicle concentrated solution have a synergistic effect, the excellent soft tissue regeneration promoting capacity is achieved, a complete autologous source is achieved, connective tissue transplantation surgery is replaced, and the application prospect is wide. The excellent performance is shown in the clinical application of soft tissue regeneration.

This invention belongs to the field of biomedicine and relates to the application of a detection reagent for a marker of mixed connective tissue diseases. This invention discloses... MEF2C Genes can serve as biomarkers for mixed connective tissue disease; they are found in the peripheral blood of patients with mixed connective tissue disease. MEF2C Gene expression levels were significantly higher in the group than in the healthy group. This invention provides a detection reagent for a mixed connective tissue disease marker, comprising an upstream primer with the nucleotide sequence shown in SEQ ID NO.3 and a downstream primer with the nucleotide sequence shown in SEQ ID NO.4, which can detect... MEF2C Gene expression levels, which can then be used to develop products for the early diagnosis of mixed connective tissue diseases.

A joint rotational exercise and stretching tool for strengthening muscles and connective tissues, particularly for shoulder internal and external rotation, with further applicability to the wrist, elbow, and hip. The device includes a mount, torque-generating mechanism, and ergonomic lever arm configured to align with anatomical joint axes. It enables concentric, eccentric, isometric, and passive modes of use. Unlike traditional tools that provide linear resistance or partial-range torque, this invention delivers continuous rotational resistance across the full arc of motion. Configurable mounting and ergonomic adjustments allow use across various joints, limb lengths, and exercise positions. Suitable for rehabilitation, injury prevention, and performance training in clinical, home, or athletic environments.

The present invention discloses a rotator cuff patch, its preparation method and application, belonging to the technical field of composite biomaterials in medical engineering. The present invention prepares an oriented nanofiber patch by electrospinning a synthetic biodegradable high polymer and a natural bioactive protein of biological origin. Calcium silicate nanoparticles are in-situ coprecipitated at the distal end of the oriented nanofiber patch, and connective tissue growth factor is loaded at the proximal end of the oriented nanofiber patch to obtain the rotator cuff patch. It is a gradient composite rotator cuff patch with a tendon-promoting region loaded with connective tissue growth factor, a cartilage-promoting region co-loaded with connective tissue growth factor and calcium silicate, and a bone-promoting region with calcium silicate deposition. It can be used for the treatment of acute rotator cuff tears, effectively realizing multi-tissue regeneration of tendon, cartilage and bone, and then synchronously completing tendon-bone interface healing and promoting rotator cuff injury repair.

The invention discloses a multi-modal fusion maternal and child medical image intelligent identification and diagnosis auxiliary system and method, and belongs to the technical field of medical health, and the system comprises a data obtaining module which is used for obtaining a medical image of a related part of a patient, the related part comprises an area corresponding to gland tissue, related processing is carried out on the medical image, and the medical image is obtained; the correlation processing includes distinguishing the medical image into an image corresponding to a gland and an image corresponding to connective tissue. According to the method, by obtaining and distinguishing gland and connective tissue medical images, extracting edge features and monitoring area changes, the lesion site can be accurately judged, the distance change between the blood vessel and the blood vessel group can be quantitatively evaluated, then the lesion development trend is scientifically judged, a comprehensive and objective diagnosis basis is provided for doctors, and the diagnosis efficiency is improved. And the diagnosis accuracy and the clinical decision scientificity are effectively improved.

Provided is an "all natural" biologic-enhancing beverage (BioBeverage), which contains, in addition to purified water, electrolytes, vitamins, anti-oxidants, micronutrients, natural fruit infusion, natural sweetener to improve taste, enhance GI Biome viability, provide antimicrobial benefits and reduce oxidative stress, Citicoline, to enhance brain / neurologic function, neurologic maintenance, repair and regeneration, CoQ10, to enhance cardiovascular energy and health, Hydroxyproline, to enhance connective tissue maintenance, repair and regeneration, and a niacinamide adenine dinucleotide (NAD+) precursor to promote overall health, including neurologic, cardiovascular and connective tissues maintenance and repair, and if present, reduce risks and symptoms of multiple neurodegenerative disorders by maintenance of healthy mitochondrial function.

Embodiments of the instant disclosure relate to an in vitro system for generating 3D skin equivalent, compositions, and methods for modeling and analyzing potential therapeutic treatments of Ehlers-Danlos Syndrome (EDS), including personalized therapies and regimens. In certain embodiments, the in vitro system can be utilized to facilitate diagnosis, drug discovery, investigation of disease mechanisms, and treatment of EDS and other connective tissue diseases. In certain embodiments, compositions, and methods for making and using matrix metalloproteinase 9 (MMP-9) inhibitors are disclosed to treat EDS. In some embodiments, a subject has hypermobility type EDS (hEDS).

The present invention relates to the field of biomedicine. Specifically, the present invention relates to an aptamer targeting a connective tissue growth factor (CTGF) protein, an aptamer conjugate targeting the CTGF protein, and a use thereof in inhibiting the activity of the CTGF protein.

The invention relates to an edible composite membrane of water-soluble dietary fiber and collagen as well as preparation and application of the edible composite membrane, and belongs to the technical field of biological materials. Preparing a culture medium from konjac glucomannan and a nitrogen source, and sterilizing; adding edible and medicinal fungus lucid ganoderma into the culture medium for culture and fermentation, and then centrifuging to remove precipitate to obtain hydrolyzed konjac oligosaccharide; after collagen is swelled, the collagen is extracted from tendon or connective tissue of an animal, or the collagen is extracted from animal skin; adding konjac oligosaccharide to obtain a film forming solution; and pouring the film forming liquid into a mold, and drying to obtain the edible composite film of the water-soluble dietary fiber and the collagen. The edible composite film has the advantages of high thermal stability, high antibacterial activity, compact and smooth surface, good moisture resistance and the like. Konjac oligosaccharide is soluble dietary fiber and has the physiological activity of reducing blood sugar and blood fat, preventing cancers and the like, and the edible film synthesized from the konjac oligosaccharide can improve the basic performance defect of collagen and can also endow the film with the health-care effect.

This invention relates to a method for producing extracellular matrix using connective tissue cell culture and to the uses of the produced extracellular matrix. More specifically, it relates to a method for culturing connective tissue cells using a serum-free and heterogeneous culture medium, which can be effectively used to increase the amount of extracellular matrix produced. Furthermore, the extracellular matrix produced by the production method of this invention is derived from human cells and does not contain any heterogeneous components such as fetal bovine serum, making it safe and useful for future development of cell therapy agents, medical supplies, medical devices for tissue treatment, or cosmetic raw materials.