34 results about "Tissue Graft" patented technology

The present disclosure pertains to membranous tissue grafts comprising one or more pre-made attachment points. The one or more pre-made attachment points may include pre-made markings and / or pre-made suture holes. The membranous tissue grafts can be in the form of a tube. The membranous tissue grafts can also be rectangular in shape and can be used in a nerve repair by wrapping the severed or damaged nerve. In some embodiments, the membranous tissue grafts are suitable for repairing severed nerves that have a short gap or no gap with a gap of less than 5 mm between the severed stumps. Accordingly, methods are provided for repairing a damaged or severed nerve by implanting the membranous tissue grafts on to the damaged or severed nerve.

A tissue graft for soft tissue repair or reconstruction comprising a sheet of a biopolymer-based matrix having a plurality of small perforations and a plurality of large perforations. The small perforations are sized to facilitate clotting and granulation tissue development within the perforations which, in turn, facilitates revascularization and cell repopulation in the patient. The large perforations are sized to reduce the occurrence of clotting and granulation tissue development within the perforations so that extravascular tissue fluids accumulating at the implant site can drain through the tissue graft. The large perforations enhance mammal tissue anchoring by permitting mammal tissue to compress into the perforations increasing mammal tissue contact area.

Described herein are tissue grafts derived from the placenta. The grafts are composed of at least one layer of amnion tissue where the epithelium layer has been substantially removed in order to expose the basement layer to host cells. By removing the epithelium layer, cells from the host can more readily interact with the cell-adhesion bio-active factors located on top of and within the basement membrane. Also described herein are methods for making and using the tissue grafts. The laminin structure of amnion tissue is nearly identical to that of native human tissue such as, for example, oral mucosa tissue. This includes high levels of laminin-5, a cell adhesion bio-active factor shown to bind gingival epithelia-cells, found throughout upper portions of the basement membrane.

The described system designed to promote the biological attachment of fibrous connective tissue to a bone implant features a bone implant with a scaffold defining a porous attachment surface, a suction port, and a manifold that distributes a negative pressure gradient across the attachment surface. The system also includes an extracorporeal vacuum pump and a percutaneous suction hose connecting the suction port of the implant to the vacuum pump. The implant is placed in the patient's body with connective tissue applied over the attachment surface, and the suction hose is attached and connected to the vacuum pump. The vacuum pump, operating outside the body, creates a negative pressure gradient over an extended period, promoting the growth of slow growing Sharpey's fibres for superior biological integration. A highly vascularised tissue graft, such as a pedicled muscle flap, may be applied adjacent to the avascular tendon to enhance endogenous growth factor migration.

A set of a cutting member (100) and a stamping member (200) for mincing biological tissue. The cutting member (100)comprising a cutter body (10) having a plurality of ducts (2) with two open ends, each duct (2) opening on one open end into a circumferential cutting blade (3) for incising a biological tissue, said blade (3) having a circular cross-section or a regular polygonal cross-section. A plurality of circumferential cutting blades form at least a portion of a cutting surface (13) of said cutter body. The cutting member (100) is arranged to cooperate with the stamping member (200) comprising a stamp body (23) with a plurality of stamp rods (22), each stamp rod being dimensioned to match a duct (2) of the cutting member (100). The disclosure further concerns a device comprising the cutting member (100) and the stamping member (200), as well as a method for generating minced tissue graft using the cutting member (100) and the stamping member (200).

Disclosed herein are three-dimensional bone tissue grafts produced from stacked demineralized bone paper. Also disclosed are methods for treating a subject using tissue grafts produced from the disclosed demineralized bone paper. Also disclosed are assay systems that involves culturing bone-promoting cells on the disclosed demineralized bone paper.

Hydrogels comprising a macromolecular matrix and water may be used to augment soft tissue of a human being, promote or support cell or tissue viability or proliferation, create space in tissue, and for other purposes. A macromolecular matrix may comprise a hyaluronic acid component crosslinked to a silk fibroin component.

A system for harvesting bone material from a bone may include a rotary cutter defining a rotary cutter longitudinal axis extending between a rotary cutter proximal end and a rotary cutter distal end. The rotary cutter may have a drive shaft configured to receive input torque, and an osteochondral cutter configured to cut the tissue and receive the tissue material in response to rotation of the osteochondral cutter under pressure against the tissue. The system may further include a bone port defining a bone port longitudinal axis extending between a bone port proximal end and a bone port distal end. The bone port may have a bone port cannulation sized to closely fit over the osteochondral cutter. At least one of the bone port proximal end and the bone port distal end may be securable to the tissue. A stratiform tissue graft may be delivered through the bone port.

Methods for producing allograft tissue by applying an antimicrobial solution to allograft tissue. The antimicrobial solution exhibits antimicrobial activity to make allograft resistant to microbial organisms, such as bacterium. Surface-modified tissue grafts prepared by these methods are also disclosed.

Embodiments relate to a living tissue graft derived from autologous cells used to provide support or to strengthen / reinforce compromised tissue. The living tissue graft comprises both cells and extracellular matrix (ECM) and overcomes problems related to foreign body responses to synthetic materials and rejection reactions to allograft tissue.

Provided are methods of making a chimeric thymus in a subject, including implanting a donor thymic functional unit tissue graft, wherein the donor thymic functional unit tissue graft includes thymic epithelial cells (TEC) and dendritic cells. The chimeric thymus is useful to induce a dual immunologic tolerance to both donor tissue antigens and tissue antigens of the subject.

A tissue graft delivery system and associated devices are described. The tissue graft delivery devices utilized a conveyor belt (122) moved by an actuator mechanism (123, 131) to shuttle a tissue graft, such as bone graft material, from a chute (111) containing the graft into a surgical site, such as an intervertebral disc space. The interactive system and devices eliminate graft packing, inefficient tissue delivery, and other difficulties arising from friction between the bone graft material and graft contact surfaces of existing prior art systems, such as bone funnels.

A dehydrated, partially perforated, placental tissue allograft that can be used in the treatment of wounds. Specifically, the dehydrated, partially perforated placental allograft has the property of being able to be cleaned during processing without disrupting the grafts structural integrity or decreasing the concentration of growth factors found within the finished graft. The dehydrated, partially perforated, placental tissue allograft is reconstituted prior to applying it to the subject and is substantially free of undesirable maternal blood contaminants.

A method of removing senescent cells from donor tissues or cells, organs, or autologous cells or tissues forms a tissue graft. The method comprises adding at least one senolytic therapy to the tissue or cells during processing or prior to storage. The senolytic therapy preferably involves any strategy to reduce senescent cells, reduce senescence phenotype, reduce factors that can cause senescent cells, target known senescence signaling (e.g. inhibitors or RNA therapeutics). The viable tissue graft comprises live tissue with enhanced biological properties. The amount of senescent cells in the graft is reduced by at least 15% as compared to an amount of senescent cells present in normal tissue. The amount of senescence-associated secretory phenotype SASPs in the graft is reduced by at least 10% as compared to an amount of senescence-associated secretory phenotype SASPs present in normal cells.

Oligoesters and polyesters comprising one or more (e.g., a plurality) of crosslinkable and / or crosslinked clickable groups (e.g., capable of or formed by click reactions, respectively). methods of making same. and uses of same. In various examples, a method forms objects by irradiating compositions comprising crosslinkable oligoesters and polyesters and, optionally, solvent (e.g., crosslink-able solvent), crosslinking reagent, and / or crosslinking initiator, with electromagnetic radiation and / or by heating the compositions. In various examples, a method forms films, fibers, and other 3D objects. In various examples, biodegradable and / or elastomeric materials comprising crosslinked oligoesters and polyesters are useful in devices (e.g., microfluidic devices, lab on a chip, drug delivery devices, and the like), scaffolds, tissue grafts, skin patches, and the like. In various examples, methods are additive manufacturing methods, (e.g., 3D printing methods, such as, for example, digital light processing (DLP)).

Microgel assemblies provide an injectable and tunable microporous scaffold for immune cell modulation. Leukocytes from graft recipients can migrate into and integrate within the microgel assembly to induce immunological tolerance for the graft without the need for chronic immunosuppressive therapy. The microgel assembly can be used to treat for tissue graft and to reduce an alloreactive immune response following a tissue graft.

The present invention relates to a vertebral system comprising a vertebral implant (2) and a plurality of inserts, said implant being designed to be implanted in a vertebral segment composed of at least two vertebrae and including a body (20) the walls whereof delimit a cavity (23) leading to the outside of the body (20) through at least one opening in at least one of said walls, at least one passage (21) passing through the implant (2) from the periphery to an upper or lower surface to receive a bone-anchoring device (1) capable of anchoring the implant (2) in at least one of said vertebrae, the system being characterized in that it includes at least two inserts selected from among the following inserts:at least one graft insert (3, 3A, 3B, 4, 5A, 5B, 6A, 6B, 6C, 6D, 202, 250) capable of being colonized by bone tissue and / or receiving at least one bone tissue graft and / or at least one substitute;and / orat least one bone-anchoring insert (210) comprising said passage (21) capable of receiving said bone-anchoring device (1).

The present application discloses a perfusion bioreactor system having an inner cavity and a scaffold with matching porosity to balance the flow of fluid through the bioreactor. The scaffold can be manufactured using additive manufacturing techniques or other manufacturing techniques to match the geometry of the defect (e.g., facial bone abnormalities). The inner cavity is manufactured in a similar manner and has a cavity that matches the shape of the scaffold to form a unified structure when assembled with the scaffold. By matching the shape of the scaffold and the inner cavity, free space within the internal volume of the bioreactor is eliminated. Stem cells can flow through the bioreactor and attach to the scaffold and then be cultured to grow a tissue graft.

A tissue graft product comprising two or more layers of material wherein each layer comprises extracellular matrix (ECM) or polymeric material and wherein the layers are laminated together by interlocking portions of one layer with portions of another layer.

Tissue grafts with reduced regenerative potential, methods of preparing such grafts, and related kits and methods of treatment are described. The method may include treating tissue with a digestion solution comprising trypsin, alpha-chymotrypsin (ACT) and optionally ethylenediaminetetraacetic acid (EDTA) to substantially remove one or more susceptible proteins from the tissue. The method may also include washing the treated tissue with a buffer solution and / or with a serine-containing serum. Nerve grafts prepared according to the disclosed methods may inhibit, or lessen (e.g., provide for reduced) neuroma formation and / or axonal outgrowth after implantation.

The present disclosure pertains to membranous tissue grafts comprising one or more pre-made attachment points. The one or more pre-made attachment points may include pre-made markings and / or pre-made suture holes. The membranous tissue grafts can be in the form of a tube. The membranous tissue grafts can also be rectangular in shape and can be used in a nerve repair by wrapping the severed or damaged nerve. In some embodiments, the membranous tissue grafts are suitable for repairing severed nerves that have a short gap or no gap with a gap of less than 5 mm between the severed stumps. Accordingly, methods are provided for repairing a damaged or severed nerve by implanting the membranous tissue grafts on to the damaged or severed nerve.

A vertebral tethering device is described herein comprising a tethering component. wherein the tethering component comprises a synthetic layer and a tissue graft layer, wherein the tethering component is affixed to vertebrae using a securing component.

The present application relates to the technical field of tissue engineering, and particularly relates to an organoid double-layer three-dimensional tissue graft for treating retinal pigment degeneration and a preparation method thereof.The organoid tissue graft of the technical scheme comprises a retinal precursor stem cell layer, a retinal pigment epithelial cell monolayer cell layer and an electrospun silk support layer arranged in sequence.The retinal precursor stem cell layer is formed by solidification of a low-temperature agarose cell suspension containing retinal precursor stem cells.The organoid tissue graft of the scheme can supplement a plurality of degenerative missing cells, can form interaction between transplanted cells and can maintain the activity of the transplanted cells.The three-dimensional retinal stereoscopic structure formed can guarantee correct nerve circuit and improve the visual function of the patient.The electrospun silk material provided by the scheme forms support for cells, and prevents deformation of the graft in the later period.The technical scheme can solve the technical problem that the treatment effect of the organoid tissue graft of the prior art on retinal pigment degeneration is not ideal, and has an ideal popularization and application prospect.

Tissue grafts with reduced regenerative potential, methods of preparing such grafts, and related kits and methods of treatment are described. The method may include treating tissue with a digestion solution comprising trypsin, alpha-chymotrypsin (ACT) and optionally ethylenediaminetetraacetic acid (EDTA) to substantially remove one or more susceptible proteins from the tissue. The method may also include washing the treated tissue with a buffer solution and / or with a serine-containing serum. Nerve grafts prepared according to the disclosed methods may inhibit, or lessen (e.g., provide for reduced) neuroma formation and / or axonal outgrowth after implantation.

A flexible bag for the preparation of an adipose tissue graft provided with a proximal end and a distal end and comprising: a front wall and a rear wall, which are connected to one another to define a receiving space on the inside; a first filter arranged inside the bag to divide the receiving space into a first chamber and a second chamber and to micro-fragment the adipose tissue to obtain a micro-fragmented adipose tissue; and a second filter arranged inside the second chamber to obtain a third chamber and to obtain the graft by separating a waste material from the micro-fragmented adipose tissue. The first filter comprises a distal edge which is connected to the front wall between the proximal end and the distal end and the second filter comprises a proximal edge which is connected to the rear wall between the proximal end and the distal end.

Devices and systems are provided for associating secondary materials with tissue grafts, both ex-situ and in-situ. The secondary materials may be medicants, an external cellular matrix factor, a cell, an enzyme, or an element or compound, such as oxygen. In a method, different secondary materials are delivered to a tissue graft via a delivery device at different times, such as at different stages of a healing process.

The present disclosure relates to methods for detecting, predicting, diagnosing, and / or monitoring the status of xenografts in transplant recipients, as well as methods for monitoring, administering, and adjusting immunosuppressive therapy. The present disclosure describes highly sensitive, non-invasive methods and kits for detecting, predicting, diagnosing, and / or monitoring the health status of transplants, as well as for detecting, predicting, and / or monitoring the transplant rejection status in recipients of organ, cell, or tissue transplants from donors, where the donor and recipient belong to different species. Such methods and kits are based on the detection and quantification of donor-specific nucleic acids, such as DNA or RNA, in body fluids, including, but not limited to, blood, serum, plasma, or urine, after transplantation.

The invention provides method for preparing amnion tissue grafts, as well as the grafts themselves. In specific embodiments, the tissue graft comprises a single layer of dried amnion from an umbilical cord.

Methods for producing allograft tissue by applying an antimicrobial solution to allograft tissue. The antimicrobial solution exhibits antimicrobial activity to make allograft resistant to microbial organisms, such as bacterium. Surface-modified tissue grafts prepared by these methods are also disclosed.