9 results about "Intact tissue" patented technology

Biological research requires the isolation and analysis of materials, such as RNA, DNA, and proteins, from tissue samples.The methods and compositions described herein allow for high-resolution imaging of large intact tissue samples, and then for the isolation of materials in a precise and location-dependent manner.The methods and compositions described herein can be used, for example, for biomarker discovery, cell population identification, pathological analysis, and the generation of expression data in specific regions of interest.

The utility model relates to pathological detection field especially relates to a pathological puncture tissue sample fixing bottle, including the container, the top outer wall of container is equipped with the thread groove, and the container is located the outer wall screw thread connection with the sealing cover at thread groove department, the bottom center place of sealing cover is equipped with the protection subassembly, and the inner wall of container is equipped with fixed component, protection subassembly includes the extension rod welded in the bottom outer wall center place of sealing cover, the storage cylinder welded in the outer wall of extension rod another end and the adding mouth of opening in the outer wall center place of storage cylinder one side, the utility model discloses the sealing cover after rotation is taken out from the connecting seat in the container interior with sealing cover, extension rod and storage cylinder upward, can take out the complete tissue sample, is convenient for the protection sample, is convenient for the quick sample taking out, improves the efficiency, liquid passes through the communication hole and contacts tissue sample and protection cotton, and protection cotton absorbs liquid and fixes tissue sample in the storage cylinder interior, avoids the shaking damage tissue sample.

Provided herein are methods for multiplexed in situ analysis of biomolecules in a tissue. In particular, provided herein are methods for multiplexed single-cell in situ protein and nucleic acid profiling in fixed or fresh tissues, and also allows the investigation of the different cell compositions and their spatial organizations in intact tissues through consecutive cycles of probe hybridization, fluorescence imaging, and signal removal.

The present disclosure provides methods and systems for the super-resolution (SR) imaging of polysomes in a single cell. Also provided by the present disclosure are methods for diagnosing a disease or disorder in a subject based on a polysome structure profile in a cell, including cells within an intact tissue. Methods of screening for or testing a candidate agent capable of modulating polysome structure are also provided by the present disclosure. The present disclosure also provides methods for treating a disease or disorder in a subject in need thereof. Sets of probes comprising oligonucleotide portions, which may be useful for performing the methods described herein, are also described by the present disclosure. The present disclosure further provides non-naturally-occurring assembled ribosome complexes comprising ribosome subunits and oligonucleotide probes. Additionally, the present disclosure provides kits comprising any of the probes described herein.

Provided herein are methods for multiplexed in situ analysis of biomolecules in a tissue. In particular, provided herein are methods for multiplexed single-cell in situ protein and nucleic acid profiling in fixed or fresh tissues, that allows the investigation of the different cell compositions and their spatial organizations in intact tissues through consecutive cycles of probe hybridization, fluorescence imaging, and signal removal.

The wart cutting device comprises a sleeve body and a sliding plate, the sliding plate is arranged at the middle end in the sleeve body in a sliding mode, and a limiting plate is fixedly arranged at the lower end in the sleeve body. According to the device, firstly, through the design of the clamping function, the wart body can be fixed in the clamped state, then through the design of the lifting function, the wart body is pulled upwards, so that the root of the wart body is fully exposed to the outside, the subsequent wart body cutting effect is ensured, and in addition, through the design of the limiting function, the wart body can be conveniently cut off. The limiting plates are arranged, so that the intact tissues at the periphery of the wart body are limited by the limiting plates, the wart body and the peripheral tissues are prevented from being pulled up, and the skin tissues at the periphery of the wart body are protected. Through the mechanical linkage design, after the wart body is lifted, the medical staff presses the connecting plates on the left side and the right side of the outer portion of the sleeve body, so that the connecting plates are in linkage with the blades to cut off the root of the wart body, then the wart body is completely cut off, and the wart body cutting convenience of the medical staff is improved.

The present disclosure provides methods and systems for the simultaneous profiling of the RNA transcriptome and translatome in a single cell. Also provided by the present disclosure are methods for diagnosing a disease or disorder in a subject based on a profile of the RNAs being translated in a cell, including cells within an intact tissue. Methods of screening for or testing a candidate agent capable of modulating translation of one or more RNAs are also provided by the present disclosure. The present disclosure also provides methods for treating a disease or disorder in a subject in need thereof. Sets of probes comprising oligonucleotide portions, which may be useful for performing the methods described herein, are also described by the present disclosure. The present disclosure further provides non-naturally- occurring assembled ribosome complexes comprising ribosome subunits and oligonucleotide probes. Additionally, the present disclosure provides kits comprising any of the probes described herein.

The present invention relates to polynucleotides comprising barcodes detectable by optical and sequencing methods, and methods of obtaining spatial and transcriptomic information of cells within intact tissue samples. In one embodiment, the polynucleotide sequence of the barcode or each segment of the barcode has an edit distance of more than 16 nucleotides relative to the endogenous human and mouse sequences. In another embodiment, the spatial information comprises location, arrangement and distance of one or more cells in the population of cells in the intact tissue sample, and the transcriptomic information comprises gene expression of one or more cells in the population of cells in the intact tissue sample.

Provided herein are devices, methods, and systems for in situ gene sequencing of a target nucleic acid in a cell in an intact tissue. Methods of screening a candidate agent to determine whether the candidate agent modulates gene expression of a nucleic acid in a cell in an intact tissue are also provided herein.