23 results about "Intact tissue" patented technology

Provided herein are devices, methods, and systems for in situ gene sequencing of a target nucleic acid in a cell in an intact tissue. Methods of screening a candidate agent to determine whether the candidate agent modulates gene expression of a nucleic acid in a cell in an intact tissue are also provided herein.

To provide methods of enhancing the robustness, rapidity, automation, and high-throughput nature of in situ sequencing techniques.SOLUTION: Provided herein are devices, methods and systems for in situ gene sequencing of a target nucleic acid in a cell in an intact tissue. Methods of screening a candidate agent to determine whether the candidate agent modulates gene expression of a nucleic acid in a cell in an intact tissue are also provided herein.SELECTED DRAWING: Figure 1-1

Provided herein are methods for multiplexed in situ analysis of biomolecules in a tissue. In particular, provided herein are methods for multiplexed single-cell in situ protein and nucleic acid profiling in fixed or fresh tissues, that allows the investigation of the different cell compositions and their spatial organizations in intact tissues through consecutive cycles of probe hybridization, fluorescence imaging, and signal removal.

Biological research requires the isolation and analysis of materials, such as RNA, DNA, and proteins, from tissue samples.The methods and compositions described herein allow for high-resolution imaging of large intact tissue samples, and then for the isolation of materials in a precise and location-dependent manner.The methods and compositions described herein can be used, for example, for biomarker discovery, cell population identification, pathological analysis, and the generation of expression data in specific regions of interest.

The present invention discloses a method for three-dimensional spatial omics of tissues based on three-dimensional coordinate spatial positioning. The method includes performing XYZ three-dimensional spatial encoding on analytes such as DNA, RNA, proteins, chromatin, and sugars in tissue cells through multiple rounds of DNA ligation reactions, and then analyzing the corresponding spatial positions and biological information through sequencing technology, thereby realizing three-dimensional spatial omics analysis of tissues. The method for three-dimensional spatial omics of tissues based on three-dimensional coordinate spatial positioning of the present invention has the advantages of convenient operation, low technical threshold, low cost, etc., can obtain the three-dimensional spatial distribution of heterogeneous cells in intact tissues and the expression of corresponding biological information, and is widely applied to research in the directions of neuroscience, pathology, tissue development, etc.

This invention provides a histologic system and method for rapidly and accurately assessing tumor margins for the presence or absence of tumor using machine learning algorithms. This affords a rapid and accurate histologic tumor readout and increase process efficiency and decreases the chance for human error. Advantageously and uniquely, the system and method allows for analyzing the tissue section as complete or incomplete as the first criteria to determine whether a tissue section is clear of tumor. A machine learning process receives whole slide images (WSI) of tissue and determines (a) if each image of the WSI contains complete / incomplete tissue samples and (b) if each image of the WSI contains tumorous tissue or an absence thereof. A reconstruction process generates a model of the tissue that maps types of tissue therein, and a display process provides results of the model or report for use and manipulation by a user.

Provided herein are methods for multiplexed in situ analysis of biomolecules in a tissue. In particular, provided herein are methods for multiplexed single-cell in situ protein and nucleic acid profiling in fixed or fresh tissues, and also allows the investigation of the different cell compositions and their spatial organizations in intact tissues through consecutive cycles of probe hybridization, fluorescence imaging, and signal removal.

Provided herein are methods and systems for sensitive and multiplexed in situ analysis of samples such as biological samples using cleavable fluorescent streptavidin. In particular, provided herein are methods for multiplexed single-cell in situ biomolecule profiling in samples, including fixed or fresh tissues, and also allows the investigation of the different cell compositions and their spatial organizations in intact tissues through consecutive cycles of probe hybridization, fluorescence imaging, and signal removal.

The present invention relates to clinical and industrial scale whole tissue sequencing. Devices, methods, and systems are provided for in situ gene sequencing of a target nucleic acid in a cell within intact tissue. The invention also provides a method of screening a candidate agent to determine whether the candidate agent is capable of modulating gene expression of a nucleic acid in a cell within intact tissue.

Provided herein are methods and systems for sensitive and multiplexed in situ analysis of samples such as biological samples using cleavable fluorescent streptavidin. In particular, provided herein are methods for multiplexed single-cell in situ biomolecule profiling in samples, including fixed or fresh tissues, and also allows the investigation of the different cell compositions and their spatial organizations in intact tissues through consecutive cycles of probe hybridization, fluorescence imaging, and signal removal.

The utility model relates to pathological detection field especially relates to a pathological puncture tissue sample fixing bottle, including the container, the top outer wall of container is equipped with the thread groove, and the container is located the outer wall screw thread connection with the sealing cover at thread groove department, the bottom center place of sealing cover is equipped with the protection subassembly, and the inner wall of container is equipped with fixed component, protection subassembly includes the extension rod welded in the bottom outer wall center place of sealing cover, the storage cylinder welded in the outer wall of extension rod another end and the adding mouth of opening in the outer wall center place of storage cylinder one side, the utility model discloses the sealing cover after rotation is taken out from the connecting seat in the container interior with sealing cover, extension rod and storage cylinder upward, can take out the complete tissue sample, is convenient for the protection sample, is convenient for the quick sample taking out, improves the efficiency, liquid passes through the communication hole and contacts tissue sample and protection cotton, and protection cotton absorbs liquid and fixes tissue sample in the storage cylinder interior, avoids the shaking damage tissue sample.

Provided herein are devices, methods, and systems for in situ gene sequencing of a target nucleic acid in a cell in an intact tissue. Methods of screening a candidate agent to determine whether the candidate agent modulates gene expression of a nucleic acid in a cell in an intact tissue are also provided herein.

Provided herein are methods for multiplexed in situ analysis of biomolecules in a tissue. In particular, provided herein are methods for multiplexed single-cell in situ protein and nucleic acid profiling in fixed or fresh tissues, and also allows the investigation of the different cell compositions and their spatial organizations in intact tissues through consecutive cycles of probe hybridization, fluorescence imaging, and signal removal.

The present disclosure provides methods and systems for the super-resolution (SR) imaging of polysomes in a single cell. Also provided by the present disclosure are methods for diagnosing a disease or disorder in a subject based on a polysome structure profile in a cell, including cells within an intact tissue. Methods of screening for or testing a candidate agent capable of modulating polysome structure are also provided by the present disclosure. The present disclosure also provides methods for treating a disease or disorder in a subject in need thereof. Sets of probes comprising oligonucleotide portions, which may be useful for performing the methods described herein, are also described by the present disclosure. The present disclosure further provides non-naturally-occurring assembled ribosome complexes comprising ribosome subunits and oligonucleotide probes. Additionally, the present disclosure provides kits comprising any of the probes described herein.

Provided herein are methods for multiplexed in situ analysis of biomolecules in a tissue. In particular, provided herein are methods for multiplexed single-cell in situ protein and nucleic acid profiling in fixed or fresh tissues, that allows the investigation of the different cell compositions and their spatial organizations in intact tissues through consecutive cycles of probe hybridization, fluorescence imaging, and signal removal.

The present invention provides a method for physically expanding a sample and spatially profiling mRNA and optionally protein in the sample. An associated kit is also provided. The methods herein are compatible with different samples, sample staining methods, and sequencing technologies, and are suitable for spatial transcriptomic and proteomic profiling of biological samples.

The utility model discloses a linear cutting anastomat device, which belongs to the technical field of medical instruments and comprises a linear cutting anastomat and an anastomosis nail bin, the anastomosis nail bin is mounted on the linear cutting anastomat, the linear cutting anastomat comprises an electric cutter, a gun barrel and clamping forceps, and the electric cutter is connected with the clamping forceps through the gun barrel; a parallel double-edge cutting assembly is arranged in the gun barrel and the electric cutter in a penetrating mode, the parallel double-edge cutting assembly comprises two blades arranged in parallel and a push rod, the two blades arranged in parallel are installed at one end of the push rod, and the end, away from the two blades, of the push rod is connected with a motor. The parallel double-edge cutting device has the advantages that the parallel double-edge cutting assembly is arranged, a complete tissue cutting edge can be directly obtained while tissue is cut, the tissue cutting edge can be rapidly taken out in an operation through reciprocating motion of the cutting assembly, the operation progress is accelerated, and the operation time is shortened; the anastomosis nail bin is widened, anastomosis tissue can be clamped and closed, and a section of complete tissue is reserved.

The wart cutting device comprises a sleeve body and a sliding plate, the sliding plate is arranged at the middle end in the sleeve body in a sliding mode, and a limiting plate is fixedly arranged at the lower end in the sleeve body. According to the device, firstly, through the design of the clamping function, the wart body can be fixed in the clamped state, then through the design of the lifting function, the wart body is pulled upwards, so that the root of the wart body is fully exposed to the outside, the subsequent wart body cutting effect is ensured, and in addition, through the design of the limiting function, the wart body can be conveniently cut off. The limiting plates are arranged, so that the intact tissues at the periphery of the wart body are limited by the limiting plates, the wart body and the peripheral tissues are prevented from being pulled up, and the skin tissues at the periphery of the wart body are protected. Through the mechanical linkage design, after the wart body is lifted, the medical staff presses the connecting plates on the left side and the right side of the outer portion of the sleeve body, so that the connecting plates are in linkage with the blades to cut off the root of the wart body, then the wart body is completely cut off, and the wart body cutting convenience of the medical staff is improved.

The present disclosure provides methods and systems for the simultaneous profiling of the RNA transcriptome and translatome in a single cell. Also provided by the present disclosure are methods for diagnosing a disease or disorder in a subject based on a profile of the RNAs being translated in a cell, including cells within an intact tissue. Methods of screening for or testing a candidate agent capable of modulating translation of one or more RNAs are also provided by the present disclosure. The present disclosure also provides methods for treating a disease or disorder in a subject in need thereof. Sets of probes comprising oligonucleotide portions, which may be useful for performing the methods described herein, are also described by the present disclosure. The present disclosure further provides non-naturally- occurring assembled ribosome complexes comprising ribosome subunits and oligonucleotide probes. Additionally, the present disclosure provides kits comprising any of the probes described herein.

The present invention relates to polynucleotides comprising barcodes detectable by optical and sequencing methods, and methods of obtaining spatial and transcriptomic information of cells within intact tissue samples. In one embodiment, the polynucleotide sequence of the barcode or each segment of the barcode has an edit distance of more than 16 nucleotides relative to the endogenous human and mouse sequences. In another embodiment, the spatial information comprises location, arrangement and distance of one or more cells in the population of cells in the intact tissue sample, and the transcriptomic information comprises gene expression of one or more cells in the population of cells in the intact tissue sample.

Provided herein are methods for multiplexed in situ analysis of biomolecules in a tissue. In particular, provided herein are methods for multiplexed single-cell in situ protein and nucleic acid profiling in fixed or fresh tissues, and also allows the investigation of the different cell compositions and their spatial organizations in intact tissues through consecutive cycles of probe hybridization, fluorescence imaging, and signal removal.

Provided herein are devices, methods, and systems for in situ gene sequencing of a target nucleic acid in a cell in an intact tissue. Methods of screening a candidate agent to determine whether the candidate agent modulates gene expression of a nucleic acid in a cell in an intact tissue are also provided herein.

Investigations of fine tissue structures, such as those in non-neural, non-bony tissues or organs, are best carried out in intact tissues. Compositions and methods for rendering tissues transparent for subsequent three-dimensional analysis are described herein. The inventive compositions as tissue clearing compositions consist of four core components: (1) homogenizing agents such as N-methylglucamine, urea, thiourea, guanidine, guanidine chloride, lithium perchlorate, ethylenediamine and its derivatives; (2) water-soluble regulators such as iohexol, sodium thiosulfate, polyethylene glycol and its derivatives; (3) lipid-soluble regulators such as 2,2'-thiodiethanol, propylene glycol and its derivatives; and (4) borate compounds such as boric acid, tetraboric acid, disodium tetraborate and its derivatives. The disclosed tissue clearing compositions are particularly suitable for non-neural, non-bony tissues or organs. These tissues or organs can be fresh, archived or recovered from paraffin-embedded tissues.