473 results about "PLGA" patented technology

The invention relates to the technical field of fertilizer preparation, and discloses an ecological balance fertilizer for yield increase and disease resistance of peanuts and a preparation method of the ecological balance fertilizer. The preparation process comprises the following steps: constructing a PLGA core containing trichoderma harzianum spores and magnesium carbonate through a micro-fluidic chip low-temperature water-in-oil emulsion method; coating bacillus amyloliquefaciens and potassium silicate with ultraviolet-induced cross-linked temperature-sensitive hydrogel to form a middle layer; loading rhizobium by using aminated mesoporous S < iO2 >, and spraying and assembling an enzyme response shell through a fluidized bed; finally, the hydroxyapatite nanorod and a binder are combined and granulated and formed through a fluidized bed. Precise controlled release of functional bacteria and nutrients is realized by constructing a temperature-sensitive-enzyme response type release system, and the activity of a fungicide is guaranteed to be preserved by virtue of trichoderma harzianum-rhizobium synergistic interaction and silicon bond linkage soil remediation in combination with a low-temperature fluidized bed process, so that a'fertilizer-bacterium-soil 'ecological cycle system is finally constructed.

The invention aims to provide a preparation method and application of an inhalation drug-loading ultrasonic driving nano motor, and can effectively solve the problems of preparation of the inhalation drug-loading ultrasonic driving nano motor and application of the inhalation drug-loading ultrasonic driving nano motor in preparation of drugs for treating IPF. Comprising the following steps: firstly preparing PLGA-PFD nanoparticles, then preparing PDA-PLGA-PFD nanoparticles, and finally preparing BNN6 / PDA-PLGA-PFD, namely the asymmetric nano motor BPPP with the ultrasonic responsiveness. The product disclosed by the invention is scientific and advanced in preparation method, good in product performance, high in atomization administration and IPF lung pathological barrier penetrating capability, high in medicine utilization rate, good in curative effect, simple and convenient to operate, wide in application prospect and huge in economic and social benefits.

The invention discloses bioactive macroporous hydrogel as well as a preparation method and application thereof. The bioactive macroporous hydrogel comprises hydrogel particles and a bioactive load loaded on the hydrogel particles, the hydrogel particles are obtained by mechanically extruding a hydrogel matrix, and the hydrogel matrix is formed by compounding sodium alginate, agarose and methacrylated hyaluronic acid through hydrogen bond crosslinking and photo-crosslinking; the bioactive load comprises: a) small extracellular vesicles of which the surfaces are modified with macrophage targeted CRV peptides; and b) a PLGA (poly (lactic-co-glycolic acid)) microsphere loaded with a cartilage inducer Kartogenin. The bioactive macroporous hydrogel disclosed by the invention has an internally communicated macroporous network, is beneficial to cell infiltration, blood vessel ingrowth and nutrient substance diffusion, and overcomes the defects that the traditional hydrogel is small in pore size and limits cell behaviors. According to the bioactive macroporous hydrogel disclosed by the invention, dual bioactive loads are adopted to synergistically promote tendon-bone healing, so that the treatment effect is better.

The invention belongs to the technical field of biological medicines and micro-nano motors, and particularly relates to a self-driven micro-motor system for treating helicobacter pylori gastritis and a preparation method of the self-driven micro-motor system. A self-driven micro-motor system with an active targeting function is constructed, the drug coated on the PLGA layer can be any substance with antibacterial activity or capable of promoting gastric mucosa repair, and the drug loading capacity and the sustained release time can be adjusted by adjusting the thickness of the PLGA interlayer. The selection of the enteric coating layer mainly considers the good electrostatic targeting property and pH response release property of the enteric coating layer, and various types of enteric coatings can be selected to adjust pH nodes released by the shell.

The invention relates to the field of ultraviolet light absorbers, and discloses a research and development method of an ultraviolet light absorber with a novel structure. Comprising the following raw materials in parts by weight: 25 to 30 parts of pyrene-triazine hybrid, 10 to 20 parts of MOF loaded benzotriazole, 15 to 20 parts of silicon dioxide coated nano zinc oxide, 10 to 15 parts of carbon quantum dot modified titanium dioxide, 8 to 12 parts of PLGA microcapsules, 7 to 13 parts of chitosan-hyaluronic acid composite film, 12 to 18 parts of Eu < 3 + > coated MOF fluorescent material, 8 to 12 parts of gibberellin-nano selenium compound and 5 to 8 parts of ionic liquid PF6. The absorption wavelength range is widened by virtue of a unique structure of a pyrene-triazine hybrid, the stability and dispersity are improved by MOF-loaded benzotriazole through MOF pore channels, the conditions of agglomeration and poor stability of nano-zinc oxide are improved by coating nano-zinc oxide with silicon dioxide, and the photoresponse range of titanium dioxide is expanded by modifying titanium dioxide with carbon quantum dots.

The invention belongs to the technical field of drug delivery, and particularly relates to an M-coated PLGA-10BX compound as well as a preparation method and application thereof. The preparation method comprises the following steps: preparing 10B-enriched boron nitride (h-10BN); extracting a cell membrane of the macrophage RAW264.7; polylactic acid-glycolic acid copolymer (PLGA) nano particles loaded with h-10BN are prepared; and finally, the bionic nano platform M (at) PLGA-10BN based on the macrophage membrane is prepared. The bionic nano-platform prepared by the invention is uniform in particle size and morphology, has relatively high biological safety, and has no obvious damage to various tissues and visceral organs. The compound can be used as a general platform for boron compound delivery, can also be used as an immune activator, is suitable for intravenous injection administration, and expands the application of boron neutron capture therapy (BNCT) in treatment of glioblastoma (GBM).

The invention discloses a ceramic artificial bone material and a preparation method thereof, and belongs to the technical field of ceramic material preparation. The method comprises the following steps: constructing an amphiphilic micelle system through a PEG-PLA (Polyethylene Glycol-Polylactic Acid) block copolymer, firstly preparing a micelle solution, then applying the micelle solution to precursor slurry preparation and PLGA pore-forming agent pretreatment (solving the problems of compatibility and agglomeration) in stages, molding and drying, and then carrying out gradient sintering (removing an auxiliary agent at low temperature and promoting densification at high temperature) to obtain the product. The method solves the problems of poor compatibility of a pore-forming agent and a precursor, powder agglomeration and uneven pore structure in a traditional process, and the obtained material has a three-dimensionally communicated and uniformly distributed micropore structure and is uniform in component.

The invention discloses bee venom-containing skin essence with small irritation and a preparation method of the bee venom-containing skin essence. The skin essence is prepared from PLGA-PEG / liposome nanoparticles, a temperature-sensitive gel matrix, a protective agent, an antioxidant, a stabilizer and ultrapure water, the preparation method comprises the following steps: preparing the PLGA-PEG / liposome nanoparticles, preparing a poloxamer 407 solution, resuspending the nanoparticles, filling and storing. According to the invention, the melittin and the snake venom-like peptide have a synergistic effect on skin wrinkle resistance, so that the skin care effect is improved; pLGA-PEG / liposome nanoparticles are matched with a poloxamer solution to construct a dual sustained-release drug system, so that the irritation of drugs to skin is reduced, the lasting effect of the drug effect is improved, and the obtained skin essence has excellent moisturizing, whitening, anti-wrinkle and antioxidant capacities and is small in irritation.

The invention relates to the technical field of spunlace non-woven materials, and particularly discloses a medical high-performance polylactic acid spunlace composite non-woven material and a preparation method thereof.The preparation method comprises the steps that firstly, polylactic acid fibers and crude natural cellulose fibers are modified, and a compatilizer PLGA-PEG-MDI segmented copolymer is prepared; the preparation process of the spunlace composite non-woven material comprises the steps that viscose is subjected to plasma activation, a compatilizer is sprayed to be loosened and mixed with modified polylactic acid fibers, cross lapping is carried out, polylactic acid added with the compatilizer is melt-blown to the surface of the lapping, and the spunlace composite non-woven material is obtained. And finally, paving the modified crude natural cellulose on the surface of the melt-blown layer to form the spunlace composite non-woven material with good antibacterial property, one-way wet permeability and degradability.

The invention relates to the technical field of drug-loaded microparticles, in particular to a preparation method and application of medical stone / PLGA composite drug-loaded microparticles, medical stone and PLGA are prepared into a structure with activated nano medical stone as a core and a PLGA polymer as a shell through the processes of ball milling, acid activation, emulsification, solvent volatilization and the like, and the medical stone and the PLGA are synergistically matched, so that the drug-loaded microparticles have the advantages that the drug-loaded microparticles are uniform in particle size distribution, and the drug-loaded microparticles have good drug-loaded performance. Firstly, the hydrogel has intelligent responsiveness, can accelerate degradation in alkaline and enzyme environments of wound surfaces, and realizes accurate release of drugs as required; secondly, the long-acting controlled release property is shown, and a dual sustained release barrier is constructed through medical stone adsorption and PLGA wrapping, so that the medicine is released for more than 72 hours at a constant speed following zero-order dynamics, and burst release is effectively avoided. And thirdly, when the drug-loaded particles are used for hydrogel, the drug-loaded particles in nanoscale are combined with the hydration effect of a hydrogel matrix, so that the efficient permeability of the drug in the cuticle is greatly improved.

The invention discloses a bipeptide modified bionic nano-vesicle as well as a preparation method and application thereof, and belongs to the field of biological medicines. The dipeptide modified bionic nano-vesicle comprises nano-particles formed by PLGA (poly (lactic-co-glycolic acid)), and the nano-particles are loaded with a medicine with a nerve protection or nerve repair effect; the surface of the nanoparticle is coated with a macrophage membrane for expressing RVG peptide and T7 peptide. The bipeptide modified bionic nano-vesicle simultaneously presents T7 peptide and RVG peptide through an engineered macrophage membrane, the T7 peptide is combined with a blood-brain barrier transferrin receptor through high affinity to realize efficient brain entry, astrocytes in the brain are specifically recognized by virtue of the RVG peptide, accurate recognition and delivery of target cells in a focus area are realized, and the bipeptide modified bionic nano-vesicle has a good application prospect. Meanwhile, the natural inflammation tropism and immune escape ability of a macrophage membrane are reserved, and the problems that a traditional drug delivery system is low in targeting precision, and cross-barrier distribution and intracerebral distribution are difficult to cooperate are solved.

The invention belongs to the field of medicine, and discloses application of POCM-NP-coated A939572 in preparation of a medicine for treating osteoporosis, the POCM-NP-coated A939572 comprises osteoclast precursor cell membrane vesicles and NP-coated A939572 loaded on the osteoclast precursor cell membrane vesicles, and the NP-coated A939572 is PLGA (poly (lactic-co-glycolic acid)) nanoparticles entrapped with the NP-coated A939572. The POCM-NP-coated A939572 obtained by loading the nanoparticles loaded with the A939572 small molecule medicine into the osteoclast precursor cell membrane vesicles has the effect of relieving the osteoporosis, can inhibit osteoclast differentiation and can be used for preventing, treating and relieving the osteoporosis.

The invention provides vitamin B12 modified microspheres capable of efficiently loading His tag recombinant protein as well as a preparation method and application of the vitamin B12 modified microspheres, and belongs to the technical field of biological medicines. The preparation method of the vitamin B12 modified microsphere for efficiently loading the His tag recombinant protein comprises the following steps: (1) carrying out esterification reaction on unsaturated fatty acid containing sulfydryl, disulfide bond or olefinic bond, saturated fatty acid and vitamin B12 to prepare an amphiphilic vitamin B12-lipid conjugate; and (2) taking the amphiphilic vitamin B12-lipid conjugate, PLGA (poly (lactic-co-glycolic acid)), a multi-sulfydryl cross-linking agent and a photoinitiator as raw materials, and preparing the raw materials into microspheres by adopting a microfluidic method, so as to obtain the vitamin B12 modified microspheres capable of efficiently loading various His tag recombinant proteins. The microsphere provided by the invention overcomes the problems of protein load stability and burst release of traditional microspheres, and various different His tag recombinant growth factors can be flexibly selected according to the requirements on different growth factors in treatment of complex bone defects caused by different pathogenesis.

The invention belongs to the technical field of biological medicine, and particularly relates to a CD44 / GSH dual-response eutectic drug delivery system and a preparation method thereof.The CD44 / GSH dual-response eutectic drug delivery system comprises a microneedle array composed of a biodegradable polymer matrix formed by hyaluronic acid (HA) and poly (lactic-co-glycolic acid) (PLGA), and the microneedle array comprises a plurality of microneedles; a microneedle comprising a pharmaceutical formulation of a resveratrol-berberine co-crystal integrated into the polymer matrix; wherein the polymer matrix comprises a disulfide bond that is a glutathione (GSH) responsive switch; wherein the hyaluronic acid is capable of specifically targeting a CD44 receptor; wherein the disulfide bond can be cleaved under the condition that the concentration of glutathione in a target tissue is increased, thereby releasing the resveratrol-berberine eutectic pharmaceutical formulation; and wherein the microneedle array is configured to percutaneously deliver the resveratrol-berberine co-crystal pharmaceutical formulation to the target tissue.

The invention discloses a special controlled-release nitrogen fertilizer containing flavonoid nutrient signal substances for corn. Comprising 1000 parts of a nitrogen fertilizer, 5-10 parts of flavonoid nutrient signal substance microcapsules and 50-100 parts of a coating material. A flavonoid nutrient signal substance and benzoic acid are dissolved in deionized water, the pH is adjusted to be alkaline, a signal substance solution is obtained, a PLGA solution is slowly poured into the signal substance solution, and a water / oil type emulsion is obtained through homogenization and emulsification; adding a polyvinyl alcohol solution into the water / oil type emulsion in a constant-temperature water bath, homogenizing and emulsifying to obtain a water / oil / water type emulsion; and freezing, centrifuging, washing and freeze-drying the water / oil / water type emulsion to obtain the flavonoid nutrient signal substance microcapsule. Compared with the traditional controlled-release fertilizer, the controlled-release nitrogen fertilizer disclosed by the invention can continuously release signal substances into soil during the growth period of corn, stimulate the growth and metabolism of root systems, promote the colonization of nitrogen-fixing microorganisms and improve the nitrogen fertilizer utilization rate of the corn.

The invention provides pet tear stain eye drops containing haematococcus pluvialis extract, the pH value of the eye drops is 6.8-7.2, the osmotic pressure is 280-320 mOsm / kg, the viscosity is 3-8 mPa.s (25 DEG C), and the eye drops comprise the following components in percentage by mass: 0.05-0.5% of haematococcus pluvialis astaxanthin extract (based on the astaxanthin content being greater than or equal to 5%), 0.05-0.5% of a pH regulator (based on the astaxanthin content being greater than or equal to 5%), and the balance of water. 1%-3% of an anti-inflammatory plant compound; 0.5%-1.2% of an isoosmotic adjusting agent; 0.2%-0.8% of a nano carrier stabilizer; 0.01%-0.1% of a pH buffer agent; compared with the prior art, the invention has the following beneficial effects: the corneal permeability of the astaxanthin is increased to 82% (measured by a Franz diffusion cell) through PLGA coating, and is increased by 5.5 times compared with the corneal permeability of the astaxanthin in a free state (15%); natural tocopherol is reserved through supercritical CO2 extraction (the content of the natural tocopherol is larger than or equal to 0.5% through HPLC detection), and the retention rate of the astaxanthin stored at 40 DEG C for 90 days is larger than or equal to 90% through Calendula quercitrin inhibits the expression of IL-6 (enzyme-linked immunosorbent assay (ELISA) detection is reduced by 76%), and chamomile bisabolol blocks a COX-2 pathway.

The invention relates to a multistage self-anchoring flexible deep brain electrical stimulation electrode and a preparation method thereof, and relates to the technical field of medical instruments. A bionic root system-octopus whisker composite framework including a main electrode base, a second-stage fractal arm and a third-stage self-anchoring tail end is adopted, intelligent materials such as carbon nano tube / PDMS composite fibers, temperature-sensitive PNIPAAm hydrogel and a degradable PLGA-gelatin composite material are fused, and a mechanical lock catch and biological fusion dual-anchoring mechanism is constructed. The distributed electrode array comprises platinum-iridium alloy, graphene / PDMS and a titanium nitride nano electrode, and cross-scale stimulation from the nuclear group level to the single cell level is achieved. The intelligent regulation and control system solves the problems of brain tissue displacement and chronic inflammation through a pressure feedback degradation and flexible interconnection technology. Compared with a traditional product, the contact area of the electrode is increased by 5-8 times, the stimulation precision reaches the single cell level, and the electrode is suitable for long-term deep brain stimulation treatment of nerve diseases such as Parkinson's disease and epilepsy and has remarkable clinical application value.

The invention relates to the technical field of drug delivery, and discloses a multilayer drug-loaded microsphere for intraocular sustained-release therapy and a preparation process thereof.The multilayer drug-loaded microsphere comprises a core layer, a middle layer and a shell layer, the core layer is composed of a polylactic acid-glycolic acid copolymer (PLGA) and a first hydrophobic drug, the middle layer is formed by wrapping the core layer with carboxymethyl chitosan, and the shell layer is composed of a first hydrophobic drug and a second hydrophobic drug. The first hydrophobic drug comprises a first therapeutic drug, the middle layer comprises a second therapeutic drug, the shell layer is composed of a polylactic acid-caprolactone copolymer PLCL and a third hydrophilic drug, and the first hydrophobic drug, the second therapeutic drug and the third hydrophilic drug are different from one another. Through the layered design of the core layer, the middle layer and the shell layer and in combination with differential degradation rates of PLGA, carboxymethyl chitosan and PLCL materials, gradient release of hydrophobic and hydrophilic drugs is achieved, a drug release curve is matched with the pathological stage of intraocular diseases, macular degeneration rapidly inhibits vascular leakage in the early stage and continuously resists inflammation in the later stage, and the effect of treating macular degeneration is achieved. And the layered slow-release synergistic treatment effect is verified.

The invention discloses a preparation method of a gradient pore-forming magnesium-containing calcium phosphate bone repair material, which comprises the following steps: S1, mixing nano-hydroxyapatite with polylactic acid-glycolic acid copolymer (PLGA) powder, adding dichloromethane as a solvent, continuously stirring to form a solution, and freeze-drying to obtain an nHA / PLGA raw material, and carrying out high-temperature fused deposition through a 3D biological printer to prepare the degradable nPLGA stent. S2, weighing the calcium phosphate bone cement powder and the magnesium metal microspheres according to a preset mass ratio, stirring the slurry and homogenizing to obtain calcium phosphate bone cement slurry containing the magnesium metal microspheres; s3, injecting the calcium phosphate bone cement slurry obtained in the step S2 into the nPLGA stent prepared in the step S1 to obtain an MC / nPLGA compound; gradient micropores are generated through hydration reaction of the magnesium microspheres, the gradient micropores and macropores formed by degradation of PLGA form a complementary structure, and the total porosity reaches 68% and is increased by 3.4 times compared with traditional CPC.

The invention discloses a PLGA (poly (lactic-co-glycolic acid))-coated curcumin nano preparation as well as a preparation method and application thereof.The PLGA and curcumin are dissolved in DMSO (dimethylsulfoxide) solutions respectively to form basic solutions with the mass concentration of 20 mg / mL respectively; the preparation method comprises the following steps: adding a basic solution of PLGA (poly (lactic-co-glycolic acid)) into a basic solution of curcumin in a mass ratio of (1: 2.5)-(1: 10) in a water bath ultrasonic state to obtain a mixed solution A; slowly adding the mixed solution A into a PVA aqueous solution with the concentration of 2% in a water bath ultrasonic state to obtain a mixed solution B; and putting the mixed solution B into an aqueous solution, and dialyzing overnight to obtain the PLGA coated curcumin nano preparation. The curcumin composition effectively overcomes the problems of poor water solubility and low oral bioavailability of curcumin, has a good protection effect on resisting PEDV infection of piglets, effectively reduces the diarrhea rate of the piglets, and can be widely applied to prevention and control of PEDV in livestock and poultry farms.

The invention belongs to the technical field of biological medicine and nanotechnology, and particularly relates to nanoparticles loaded with curcumin and antibacterial peptide as well as a preparation method and application of the nanoparticles. The nanoparticles loaded with the curcumin and the antibacterial peptide are prepared by the following steps: taking PEI-PLGA and the curcumin as raw materials, and preparing PEI-PLGA-Cur nanoparticles; pEI-PLGA-Cur nanoparticles and the antibacterial peptide are used as raw materials, and the nanoparticles loaded with the curcumin and the antibacterial peptide are prepared. The nanoparticles loaded with the curcumin and the antibacterial peptide provided by the invention have a photodynamic therapy effect, the drug loading capacity of the curcumin in the nanoparticles is relatively high, the loading rate of the antibacterial peptide is relatively large, auxiliary materials are safe, the preparation process is simple, the particle size of the nanoparticles is relatively small and uniform, the blood compatibility is good, the nanoparticles can be used for treating infection of drug-resistant bacteria, and the application prospect is wide. The potential of becoming a novel nano antibacterial drug and the clinical application prospect are realized.

The invention discloses a degradable electrospinning dressing, and belongs to the technical field of biological materials.The degradable electrospinning dressing is prepared by dispersing aminosilane modified FeO in a chitosan solution, adding a gelatin solution, stirring and mixing evenly, conducting gradient freezing under the action of a rotating magnetic field, conducting permeation with a PLGA solution, then conducting electrospinning, conducting crosslinking with a genipin solution, conducting washing and conducting drying. And sterilizing to obtain the degradable electrospinning dressing. According to the degradable electrospinning dressing prepared by the preparation method disclosed by the invention, an electrospinning auxiliary material is obtained through three-layer gradient electrospinning by modifying a Fe3O4 enhanced chitosan-gelatin composite base material with amino silane and combining magnetic field assistance and gradient freezing forming, a bionic fiber structure is constructed, wound exudation dynamic balance and long-acting antibiosis are realized, the optimal moisture balance of a wound part is kept, and the antibacterial property of the wound part is improved. And wound healing is effectively promoted.

The disclosure relates to microparticles and nanoparticles comprising a polymer matrix comprising an uncapped polymer and a net positively charged therapeutic agent at neutral pH. More particularly the disclosure relates to PLGA and / or PLA particles comprising an uncapped polymer for extended, controlled release of positively charged proteins or peptides at neutral pH. Methods of making the particles and administering the particles are also provided.

The invention discloses a compound microorganism freeze-drying protective agent, a preparation method of the compound microorganism freeze-drying protective agent and a use method of the compound microorganism freeze-drying protective agent. Comprising the following raw materials in parts by weight: 0.03 part of disodium ethylene diamine tetraacetate, 5 to 15 parts of trehalose, 2 to 5 parts of sorbitol, 0.5 to 2 parts of ascorbic acid, 0.05 to 0.2 part of N-acetyl-L-cysteine, 0.1 to 0.5 part of polylactic acid-glycolic acid copolymer, 0.01 to 0.05 part of alpha-tocopherol and 1 to 3 parts of bentonite. The PLGA, the alpha-tocopherol and the NAC construct a three-stage synergistic anti-oxidation system from outside to inside: the PLGA is externally blocked, so that rapid consumption of an antioxidant is avoided, and slow release is realized; the alpha-tocopherol realizes oxidative damage repair of cell membranes; nAC maintains the internal reduction environment of cells, reduces oxidative stress and can regenerate alpha-tocopherol at the same time, and reutilization of resources is achieved.

This invention belongs to the field of drug carrier technology and discloses a brain-targeted nanocarrier, its preparation method, and application. The primary raw materials for preparing the brain-targeted nanocarrier include PLGA-PEG-NHS, D-mannosamine, and a sulfide donor. This brain-targeted nanocarrier utilizes the dual targeting effects of mannose and sulfide to not only target the brain but also effectively improve blood-brain barrier permeability. With its high targeting and high permeability, it can be used as a carrier to effectively deliver therapeutic drugs to the brain to exert therapeutic effects, and has promising application prospects.

The invention provides a nano drug delivery system, which comprises (a) an active component, which comprises an anti-tumor active substance or a mixture of the anti-tumor active substance and a substance capable of synergistically generating an anti-tumor effect with the anti-tumor active substance; (b) a carrier material, wherein the carrier material comprises an mPEG-PLGA-HA material which is modified or not modified with a PD-1 or PD-L1 monoclonal antibody; optionally (c) a lipid material selected from the group consisting of lecithin (PC), cephalin (PE), mPEG-PE, mPEG-DSPE, mPEG-DPPE, DOP-DEDA, or a combination thereof; and the active ingredient is wrapped by the carrier material. The nano drug delivery system has transmembrane capability, and can deliver active components to the outside of cells, cytoplasm and / or cell nucleus.

The present invention discloses a PLGA-minoxidil microneedle with near-infrared response and a preparation method thereof. The PLGA-minoxidil microneedle with near-infrared response comprises: PLGA-minoxidil microspheres, lanthanum hexaboride nanoparticles, a matrix, and a needle body; wherein the PLGA-minoxidil microspheres and lanthanum hexaboride nanoparticles are distributed in the needle body; and the materials of the matrix and the needle body are a combination of PVA 1788 and PVA 1799. The present invention combines PLGA-minoxidil microspheres with hydrogel microneedles with near-infrared response. In terms of microstructure, the microporous structure of the microspheres and the near-infrared response of the lanthanum hexaboride nanoparticles cooperate to achieve controlled release of MXD, effectively reduce the side effects of the drug, better maintain the drug concentration in the body, and achieve better therapeutic effects. Macroscopically, the prepared hydrogel system has good mechanical properties and can provide a stable drug delivery channel and drug delivery efficiency.

The preparation method comprises the following steps: modifying and connecting IL-15 and PD-1 protein molecules by using biotin, modifying PLGA-PEG by using avidin, and finally preparing the double-targeting nano-micelle through specific binding of the biotin and the avidin and a self-assembly effect of the PLGA-PEG. The dual-targeting nano-micelle IL-15 / PD-1 / PEG-PLGA, which can efficiently target and activate NK cells and target surface high-expression PD-L1 tumor cells, is synthesized and prepared. The mole number of IL-15 and PD-1 loaded on the surface of the dual-targeting nano-micelle is close to 1: 1, and the dual-targeting nano-micelle is regular in form, uniform in dispersion and in the shape of a sphere with the particle size of 254.76 + / -28.02 nm. Meanwhile, it is verified that the dual-targeting nano-micelle IL-15 / PD-1 / PEG-PLGA can effectively improve the recognition and killing efficiency of the NK cells on tumor cells in vivo and in vitro.

The invention describes an extended-release composition comprising PLGA or PLA polymeric microspheres comprising an isoxazoline, preferably sarolaner, and a macrocyclic lactone, preferably moxidectin, and wherein the composition further comprises at least one pharmaceutically acceptable excipient; and uses thereof.

The invention discloses a gradient degradation type personalized absorbable sternum fracture fixing plate and a preparation process, and belongs to the technical field of medical implant materials. Comprising an anatomical adaptive plate body and an integrated locking assembly, the plate body is of a partitioned structure of a middle supporting area and an edge buffering area, a gradient pore array penetrating through the thickness direction is formed in the surface of the plate body, and a hydroxyapatite-collagen composite coating is loaded on the inner wall of a pore; the plate body is made of a composite absorbable material through 3D printing forming and hot pressing post-treatment, and the composite absorbable material comprises, by weight, 60%-70% of polylactic acid-glycolic acid copolymer (PLGA, LA / GA = 75 / 25), 15%-20% of silanized magnesium alloy microwires, 5%-10% of hydroxyapatite nano-particles and 5%-10% of polycaprolactone (PCL). Space-time matching of the degradation rate and the bone healing process is achieved through the partition gradient structure, the magnesium alloy microwires enhance the mechanical property and release active ions to promote osteogenesis, and the method has the advantages of personalized adaptation, gradient degradation, bone regeneration promotion and the like.