808 results about "PLGA" patented technology

The invention relates to the technical field of fertilizer preparation, and discloses an ecological balance fertilizer for yield increase and disease resistance of peanuts and a preparation method of the ecological balance fertilizer. The preparation process comprises the following steps: constructing a PLGA core containing trichoderma harzianum spores and magnesium carbonate through a micro-fluidic chip low-temperature water-in-oil emulsion method; coating bacillus amyloliquefaciens and potassium silicate with ultraviolet-induced cross-linked temperature-sensitive hydrogel to form a middle layer; loading rhizobium by using aminated mesoporous S < iO2 >, and spraying and assembling an enzyme response shell through a fluidized bed; finally, the hydroxyapatite nanorod and a binder are combined and granulated and formed through a fluidized bed. Precise controlled release of functional bacteria and nutrients is realized by constructing a temperature-sensitive-enzyme response type release system, and the activity of a fungicide is guaranteed to be preserved by virtue of trichoderma harzianum-rhizobium synergistic interaction and silicon bond linkage soil remediation in combination with a low-temperature fluidized bed process, so that a'fertilizer-bacterium-soil 'ecological cycle system is finally constructed.

The invention relates to a pathologically responsive nano-carrier as well as a preparation method and application thereof, belongs to the technical field of biological medicines, and aims to solve the problems that in the prior art, nano-particles are enriched in livers and kidneys in quantity, the risk of damaging organs and tissues exists, toxic and side effects on patients with spinal cord injury are large, the treatment effect is not obvious and the like. The carrier is a self-assembled nanoparticle which takes PLGA as a skeleton structure, is externally modified with hyaluronic acid and is connected with RVG29 through a diselenide bond. The carrier can load an active substance, such as curcumin, can be used for treating spinal cord injury, and can target spinal cord tissues through intravenous administration, penetrate a blood spinal cord barrier, target CD44 through HA at the early stage of injury, increase immune cell uptake and inhibit inflammatory infiltration and inflammatory cascade. In the late stage of injury, the nerve cell uptake is increased through RVG29 targeted acetylcholine receptor (nAchR), and nerve repair and regeneration are promoted.

The invention discloses a method for preparing a fully degradable hydrophilic / hydrophobic double-layer nanofiber membrane based on an electrostatic spinning method. The preparation method comprises the following steps: selecting a degradable high polymer material, taking hexafluoroisopropanol as a solvent, preparing a hydrophilic layer spinning solution from polylactic acid-glycolic acid copolymer (PLGA), methoxypolyethylene glycol (mPEG) and carbon dots (CDs) with antibacterial and anti-inflammatory characteristics, preparing a hydrophobic layer spinning solution from polycaprolactone (PCL), preparing a hydrophilic layer spinning solution from the hydrophilic layer spinning solution, preparing a hydrophilic layer spinning solution from the hydrophobic layer spinning solution, and preparing a hydrophilic layer spinning solution from the hydrophilic layer spinning solution and the hydrophobic layer spinning solution; and preparing the hydrophilic / hydrophobic double-layer nanofiber membrane by adopting an electrostatic spinning machine. The preparation method is simple, and the prepared double-layer nanofiber membrane is uniform in fiber diameter distribution and good in hydrophilic / hydrophobic layer effect and has a good application prospect in the field of biology.

The invention belongs to the technical field of medicine preparation, and relates to PLGA gel used as an injectable long-acting local anesthesia medicine as well as application and a preparation method of the PLGA gel. The PLGA gel comprises the PLGA gel which is loaded with superparamagnetic Fe3O4 particles and a small molecule local anesthetic, namely, levobupivacaine (Levobupi). The PLGA hydrogel containing Fe3O4 and levobupivacaine can be used as an injectable long-acting local anesthesia medicine, and the hydrogel can be changed from liquid to solid after being injected into a living body, so that the release efficiency of the medicine is slowed down, and the local action time of the hydrogel is prolonged. In addition, the hydrogel can undergo a phase change from a solid state to a liquid state under magnetocaloric therapy (MHT), increase pore diameter, promote release of levobupivacaine, and provide enhanced pain relief in vivo. Compared with the prior art, the invention has the beneficial effects of simple synthesis and easy industrialization. The drug release rate is adjusted through the phase change of the hydrogel, super-long-acting analgesia is realized, and the analgesia time is gt; 20 days. The gel can be loaded with various local anesthesia drugs such as L-bupivacaine, and pain management can be carried out on various pain scenes clinically.

The invention provides a piezoelectric composite stent, a preparation method thereof and application of the piezoelectric composite stent in tracheal defect repair, and belongs to the technical field of tissue engineering. The preparation method of the piezoelectric composite stent comprises the following steps: (1) immersing a PLLA stent into an FF polypeptide solution to prepare a PLLA-FF stent; (2) the PLLA-FF stent is immersed in a tannic acid solution, and a PLLA-FF-TA composite stent is prepared; (3) preparing oxygen-containing PLGA (poly (lactic-co-glycolic acid)) nano microbubbles, and mixing the GelMA with the oxygen-containing PLGA nano microbubbles to obtain a Gel-NB hydrogel precursor; (4) injecting the Gel-NB hydrogel precursor into the PLLA-FF-TA composite scaffold, and carrying out ultraviolet light cross-linking on the Gel-NB hydrogel precursor and the PLLA-FF-TA composite scaffold; and carrying out ultrasonic irradiation to obtain the PLLA-FF-TA (at) Gel-Nb + US composite scaffold. The piezoelectric hydrogel stent is successfully prepared, can be well used for tracheal defect repair, has the advantages of good degradation performance, high piezoelectric performance, long-term stability and high strength, and is beneficial to clinical transformation and application.

The invention aims to provide a preparation method and application of an inhalation drug-loading ultrasonic driving nano motor, and can effectively solve the problems of preparation of the inhalation drug-loading ultrasonic driving nano motor and application of the inhalation drug-loading ultrasonic driving nano motor in preparation of drugs for treating IPF. Comprising the following steps: firstly preparing PLGA-PFD nanoparticles, then preparing PDA-PLGA-PFD nanoparticles, and finally preparing BNN6 / PDA-PLGA-PFD, namely the asymmetric nano motor BPPP with the ultrasonic responsiveness. The product disclosed by the invention is scientific and advanced in preparation method, good in product performance, high in atomization administration and IPF lung pathological barrier penetrating capability, high in medicine utilization rate, good in curative effect, simple and convenient to operate, wide in application prospect and huge in economic and social benefits.

The invention discloses a range-limited light-operated anti-tumor composite hydrogel drug reservoir and a preparation method and application thereof.The light-responsive drug-loaded nano hydrogel is loaded into a temperature-sensitive hydrogel system to form the range-limited hydrogel drug reservoir with light-operated drug release, the preparation method comprises the steps that PEG, LA and GA are added into a three-necked flask for an oil bath reaction, and the temperature-sensitive hydrogel system is obtained; after the reaction, purifying and freeze-drying to obtain a PLGA-PEG-PLGA block copolymer; the PLGA-PEG-PLGA segmented copolymer is dissolved in ultrapure water, and a PLGA-PEG-PLGA sol is obtained; and dissolving the drug-loaded PDA (at) P (NIPAAm-co-AM) nano hydrogel into the PLGA-PEG-PLGA sol, so as to obtain the confined light-operated anti-tumor composite hydrogel drug reservoir. The confined light-operated anti-tumor composite hydrogel drug reservoir has light-responsive drug delivery performance, not only has space-time controllability of drug release, but also weakens the problems of photothermal effect and the like in light-assisted therapy, so that accurate tumor therapy can be realized.

The invention discloses a multi-layer sustained-release microsphere with an anti-wrinkle effect as well as a preparation method and application of the multi-layer sustained-release microsphere. The multilayer sustained release microsphere provided by the invention is prepared from the following components in percentage by weight: 5 to 15 percent of alligator oil, 65 to 80 percent of polylactic acid-glycolic acid copolymer (PLGA), 5 to 15 percent of chitosan and 0.1 to 5 percent of natural antioxidant. The multi-layer sustained release microsphere sequentially comprises an alligator oil-PLGA core sphere, a natural antioxidant-PLGA middle layer and a chitosan outer layer from inside to outside. The multilayer sustained-release microsphere prepared by adopting an electrostatic spinning technology has a porous multilayer structure, can realize accurate sustained release of different active components in each layer, prolongs the duration of a repairing effect, effectively reduces a burst release effect, and can ensure the stability of each active substance through layering treatment among the active substances, so that the stability of the active substances is improved. All the layers are released according to a specific rate and synergistically act on the skin, so that the utilization rate of active ingredients can be increased, waste is reduced, and meanwhile, the irritation risk to the skin is also reduced.

The invention belongs to the technical field of cosmetics, and discloses skin-tightening and anti-wrinkle essence. The skin-tightening anti-wrinkle essence is prepared from the following raw materials in percentage by mass: 1.5 to 2.5 percent of acetyl hexapeptide-8, 2.0 to 3.0 percent of palmitoyl pentapeptide-4, 3.0 percent of hydroxypropyl tetrahydropyrantriol, 2.0 percent of lipidosome-coated vitamin C ethyl ether, 0.5 percent of resveratrol PLGA microspheres, 0.8 percent of ceramide NP, 1.2 percent of pachymaran, 5.0 percent of glyceryl polyether-26, 0.3 percent of sodium hyaluronate, 0.5 percent of p-hydroxyacetophenone and 1, 3-propanediol, 0.5 percent of sodium hyaluronate, 0.5 percent of sodium hyaluronate, 0.5 percent of sodium hyaluronate and the balance of water. The 1, 2-hexanediol accounts for 0.6%, and the balance is deionized water. Multi-target anti-wrinkle, deep moisturizing and long-acting repairing are achieved by scientifically compounding active ingredients such as acetyl hexapeptide-8 and palmitoyl pentapeptide-4 and combining a lipidosome wrapping technology and a nano-microsphere slow release technology, the preparation process comprises the steps of lipidosome homogenization, nano-microsphere multiple emulsion method and low-temperature emulsification, the ingredient stability and transdermal efficiency are ensured, and the skin care effect is good. Experiments prove that the essence can significantly improve skin elasticity and reduce wrinkles, and has a significant anti-aging effect.

The invention provides a quick-release alfacalcidol soft capsule and a preparation method thereof, and relates to the technical field of pharmaceutical preparations, the preparation raw materials comprise a PLGA microsphere content containing vitamin D3 and a capsule shell liquid containing beta-cyclodextrin, the preparation method of the quick-release alfacalcidol soft capsule comprises the following steps: preparing the capsule content and the capsule shell liquid into the soft capsule by using a full-automatic soft capsule machine, shaping in cold air at the temperature of 20 DEG C and the humidity of 45%, washing pills, drying and packaging to obtain the alfacalcidol soft capsule.

The invention discloses bioactive macroporous hydrogel as well as a preparation method and application thereof. The bioactive macroporous hydrogel comprises hydrogel particles and a bioactive load loaded on the hydrogel particles, the hydrogel particles are obtained by mechanically extruding a hydrogel matrix, and the hydrogel matrix is formed by compounding sodium alginate, agarose and methacrylated hyaluronic acid through hydrogen bond crosslinking and photo-crosslinking; the bioactive load comprises: a) small extracellular vesicles of which the surfaces are modified with macrophage targeted CRV peptides; and b) a PLGA (poly (lactic-co-glycolic acid)) microsphere loaded with a cartilage inducer Kartogenin. The bioactive macroporous hydrogel disclosed by the invention has an internally communicated macroporous network, is beneficial to cell infiltration, blood vessel ingrowth and nutrient substance diffusion, and overcomes the defects that the traditional hydrogel is small in pore size and limits cell behaviors. According to the bioactive macroporous hydrogel disclosed by the invention, dual bioactive loads are adopted to synergistically promote tendon-bone healing, so that the treatment effect is better.

The invention discloses a lung cancer cell membrane bionic targeting precious metal-based PLGA nano drug-loaded particle, which comprises a drug-loaded inner core and a lung cancer cell membrane coating the inner core, the drug-loaded inner core structure sequentially comprises an inner core layer and an intermediate core layer, the inner core layer is a chemotherapeutic drug cisplatin, the intermediate core layer is PLGA with good biocompatibility, and the drug-loaded inner core structure sequentially comprises an outer core layer and an inner core layer. The outer surface of the PLGA is modified with noble metal, and the noble metal is one or two of gold, ruthenium and platinum. The invention also discloses a preparation method of the lung cancer cell membrane bionic targeting noble metal-based PLGA nano drug-loaded particle. According to the drug-loaded nanoparticle and the preparation method thereof, tumor cells can be effectively targeted, heat is generated under near-infrared irradiation, the drug cis-platinum loaded in the PLGA NPs can be exposed and released from the inside, and the effect of chemotherapy-photo-thermal synergistic treatment is achieved.

The invention belongs to the technical field of biological medicines and micro-nano motors, and particularly relates to a self-driven micro-motor system for treating helicobacter pylori gastritis and a preparation method of the self-driven micro-motor system. A self-driven micro-motor system with an active targeting function is constructed, the drug coated on the PLGA layer can be any substance with antibacterial activity or capable of promoting gastric mucosa repair, and the drug loading capacity and the sustained release time can be adjusted by adjusting the thickness of the PLGA interlayer. The selection of the enteric coating layer mainly considers the good electrostatic targeting property and pH response release property of the enteric coating layer, and various types of enteric coatings can be selected to adjust pH nodes released by the shell.

The invention relates to the field of ultraviolet light absorbers, and discloses a research and development method of an ultraviolet light absorber with a novel structure. Comprising the following raw materials in parts by weight: 25 to 30 parts of pyrene-triazine hybrid, 10 to 20 parts of MOF loaded benzotriazole, 15 to 20 parts of silicon dioxide coated nano zinc oxide, 10 to 15 parts of carbon quantum dot modified titanium dioxide, 8 to 12 parts of PLGA microcapsules, 7 to 13 parts of chitosan-hyaluronic acid composite film, 12 to 18 parts of Eu < 3 + > coated MOF fluorescent material, 8 to 12 parts of gibberellin-nano selenium compound and 5 to 8 parts of ionic liquid PF6. The absorption wavelength range is widened by virtue of a unique structure of a pyrene-triazine hybrid, the stability and dispersity are improved by MOF-loaded benzotriazole through MOF pore channels, the conditions of agglomeration and poor stability of nano-zinc oxide are improved by coating nano-zinc oxide with silicon dioxide, and the photoresponse range of titanium dioxide is expanded by modifying titanium dioxide with carbon quantum dots.

The invention belongs to the technical field of medical machinery, and particularly relates to an inflammation response type controllable degradation antibacterial body stent and a preparation method thereof.The preparation method of the antibacterial body stent comprises the following steps that PEG is heated in an oil bath in a vacuum and sealed environment and stirred for 1-3 h, the mixture is cooled to the room temperature, protective gas is introduced, and the antibacterial body stent is obtained. Sequentially adding lactide, glycolide and stannous iso-octoate into PEG (Polyethylene Glycol), continuously heating in the oil bath and stirring for 6-8 hours to obtain PEG-PLGA sol; adding the PEG-PLGA sol into dichloromethane for dissolving to obtain a dichloromethane solution of the PEG-PLGA, and adding an ice diethyl ether solution into the dichloromethane solution of the PEG-PLGA, so that the PEG-PLGA is separated out from the mixed solution. L-lysine (Lys) is grafted to a PEG-PLGA molecular chain end, a topological structure of Ag nanocrystals is formed on the surface, Lys-PEG-PLGA / AgCl particles are prepared, the antibacterial body stent is prepared through a 3D printing technology, the matching degree of the antibacterial body stent and an appendix focus in a patient is improved, and meanwhile controllable degradation is achieved with pH adjustment as an anchor point.

The invention belongs to the technical field of drug delivery, and particularly relates to an M-coated PLGA-10BX compound as well as a preparation method and application thereof. The preparation method comprises the following steps: preparing 10B-enriched boron nitride (h-10BN); extracting a cell membrane of the macrophage RAW264.7; polylactic acid-glycolic acid copolymer (PLGA) nano particles loaded with h-10BN are prepared; and finally, the bionic nano platform M (at) PLGA-10BN based on the macrophage membrane is prepared. The bionic nano-platform prepared by the invention is uniform in particle size and morphology, has relatively high biological safety, and has no obvious damage to various tissues and visceral organs. The compound can be used as a general platform for boron compound delivery, can also be used as an immune activator, is suitable for intravenous injection administration, and expands the application of boron neutron capture therapy (BNCT) in treatment of glioblastoma (GBM).

The invention provides a preparation method of fusidic acid emulsifiable paste, and relates to the field of pharmaceutical preparations, and the preparation method comprises the following steps: step 1, preparation of fusidic acid nanoparticles, step 2, preparation of an oil phase of the emulsifiable paste, step 3, preparation of a water phase of the emulsifiable paste, step 4, emulsification, and step 5, mixing. According to the fusidic acid nanoparticles, firstly, a composite carrier is prepared, then fusidic acid, PLGA, the composite carrier and absolute ethyl alcohol are mixed, a nanoparticle oil phase is obtained, then PVA, PVP, sodium dodecyl glucoside sulfonate and dipotassium ethylenediaminetetraacetate are mixed with deionized water, a nanoparticle water phase is obtained, and finally a nanoparticle suspension is obtained through mixing. The fusidic acid emulsifiable paste prepared by the method can effectively solve the problems that the traditional emulsifiable paste bulk drugs are easy to agglomerate, the drug molecule particle size is large and the like, and the transdermal absorption rate and bioavailability of fusidic acid are improved, so that the antibacterial effect is enhanced.

The invention belongs to the technical field of drug sustained release, and particularly relates to a preparation method and application of a BMP-2 controllable sustained-release flocculent body based on lysozyme self-regulation. The preparation method comprises the following steps: preparing a chitosan solution; sequentially adding a protein stabilizer and glycerol into the chitosan solution obtained in the step A1 to prepare a chitosan composite solution; obtaining a non-specific binding solution; preparing BMP-2 / chitosan solid particles by adopting a freeze drying method; the preparation method comprises the following steps: preparing a PLGA / PEG composite solution to obtain a suspension, and carrying out post-treatment on the suspension to form the BMP-2 controllable slow-release flocculent. The carrier in the BMP-2 controllable slow-release flocculent body prepared by the preparation method can be specifically degraded by lysozyme in a human body, so that the release amount and the release speed of BMP-2 can be automatically adjusted according to a bone healing process, and long-time continuous release of the BMP-2 controllable slow-release flocculent body is realized. The problems that in the prior art, the slow release effect of a hydrophilic material on BMP-2 is poor, sudden release of factors is easily formed, and the slow release time and the slow release dosage are not controllable are solved.

A radioactive thermogel suspension, including a thermogel and a plurality of insoluble radioactive isotope phosphate particles, such as yttrium phosphate, suspended in the thermogel. The thermogel is PLGA-g-PEG. The thermogel contains less than 65 ppm stannous octanoate. The plurality of radioactive yttrium phosphate particles are between 0.03 μm and 10 μm in diameter. The plurality of radioactive yttrium phosphate particles are generally spherical. The YPO4 particle concentration is in the range of 3 mg / ml to 100 mg / ml.

The present invention provides a conjugate comprising: (a) a CNS targeting ligand, (b) a hydrophilic polymer of polyethylene glycol (PEG), polylactic acid (PLA), polylactic acid-co-glycolic acid (PLGA), or glucan, and (c) a flavonoid. The present invention also provides a micelle nanoparticle composition comprising: (a) an outer shell comprising the conjugate, optionally (b) an inner shell comprising oligomeric (-)-epigallocatechin gallate (OEGCG), and optionally (c) a CNS disease treatment molecule encapsulated in the inner shell. The invention also provides a method of treating a CNS disease by administering to a subject an effective amount of a nanoparticle composition of the invention. The CNS targeting ligand targets a CNS tissue and delivers an active ingredient to the CNS tissue for treating a CNS pathological condition.

The invention discloses a ceramic artificial bone material and a preparation method thereof, and belongs to the technical field of ceramic material preparation. The method comprises the following steps: constructing an amphiphilic micelle system through a PEG-PLA (Polyethylene Glycol-Polylactic Acid) block copolymer, firstly preparing a micelle solution, then applying the micelle solution to precursor slurry preparation and PLGA pore-forming agent pretreatment (solving the problems of compatibility and agglomeration) in stages, molding and drying, and then carrying out gradient sintering (removing an auxiliary agent at low temperature and promoting densification at high temperature) to obtain the product. The method solves the problems of poor compatibility of a pore-forming agent and a precursor, powder agglomeration and uneven pore structure in a traditional process, and the obtained material has a three-dimensionally communicated and uniformly distributed micropore structure and is uniform in component.

The invention discloses bee venom-containing skin essence with small irritation and a preparation method of the bee venom-containing skin essence. The skin essence is prepared from PLGA-PEG / liposome nanoparticles, a temperature-sensitive gel matrix, a protective agent, an antioxidant, a stabilizer and ultrapure water, the preparation method comprises the following steps: preparing the PLGA-PEG / liposome nanoparticles, preparing a poloxamer 407 solution, resuspending the nanoparticles, filling and storing. According to the invention, the melittin and the snake venom-like peptide have a synergistic effect on skin wrinkle resistance, so that the skin care effect is improved; pLGA-PEG / liposome nanoparticles are matched with a poloxamer solution to construct a dual sustained-release drug system, so that the irritation of drugs to skin is reduced, the lasting effect of the drug effect is improved, and the obtained skin essence has excellent moisturizing, whitening, anti-wrinkle and antioxidant capacities and is small in irritation.

The invention relates to the technical field of spunlace non-woven materials, and particularly discloses a medical high-performance polylactic acid spunlace composite non-woven material and a preparation method thereof.The preparation method comprises the steps that firstly, polylactic acid fibers and crude natural cellulose fibers are modified, and a compatilizer PLGA-PEG-MDI segmented copolymer is prepared; the preparation process of the spunlace composite non-woven material comprises the steps that viscose is subjected to plasma activation, a compatilizer is sprayed to be loosened and mixed with modified polylactic acid fibers, cross lapping is carried out, polylactic acid added with the compatilizer is melt-blown to the surface of the lapping, and the spunlace composite non-woven material is obtained. And finally, paving the modified crude natural cellulose on the surface of the melt-blown layer to form the spunlace composite non-woven material with good antibacterial property, one-way wet permeability and degradability.

The invention provides a 3D printing bone scaffold based on a TPMS structure, the bone scaffold is prepared by taking Ti-6Al-4V powder as a building raw material through a 3D printing mode, the bone scaffold is a TPMS G unit structure, the aperture range is 420-630 microns, the inner layer porosity is 55%, the peripheral porosity is 90%, the diameter of the scaffold is 15 mm, and the height of the scaffold is 2 mm. Moxifloxacin / rifampicin PLGA microspheres and BMP-2 are loaded in the bone scaffold and are fixed by hydrogel, so that the 3D printing anti-infection bone scaffold based on the TPMS structure is formed. The prepared 3D printing anti-infection bone scaffold highlights excellent performance in all aspects, has good capacity of resisting infection and promoting bone formation, and provides a new material and thought for treatment of infectious bone defects. In addition, the invention further discloses a preparation method and application of the stent.

The invention relates to the technical field of drug-loaded microparticles, in particular to a preparation method and application of medical stone / PLGA composite drug-loaded microparticles, medical stone and PLGA are prepared into a structure with activated nano medical stone as a core and a PLGA polymer as a shell through the processes of ball milling, acid activation, emulsification, solvent volatilization and the like, and the medical stone and the PLGA are synergistically matched, so that the drug-loaded microparticles have the advantages that the drug-loaded microparticles are uniform in particle size distribution, and the drug-loaded microparticles have good drug-loaded performance. Firstly, the hydrogel has intelligent responsiveness, can accelerate degradation in alkaline and enzyme environments of wound surfaces, and realizes accurate release of drugs as required; secondly, the long-acting controlled release property is shown, and a dual sustained release barrier is constructed through medical stone adsorption and PLGA wrapping, so that the medicine is released for more than 72 hours at a constant speed following zero-order dynamics, and burst release is effectively avoided. And thirdly, when the drug-loaded particles are used for hydrogel, the drug-loaded particles in nanoscale are combined with the hydration effect of a hydrogel matrix, so that the efficient permeability of the drug in the cuticle is greatly improved.

The invention discloses a bipeptide modified bionic nano-vesicle as well as a preparation method and application thereof, and belongs to the field of biological medicines. The dipeptide modified bionic nano-vesicle comprises nano-particles formed by PLGA (poly (lactic-co-glycolic acid)), and the nano-particles are loaded with a medicine with a nerve protection or nerve repair effect; the surface of the nanoparticle is coated with a macrophage membrane for expressing RVG peptide and T7 peptide. The bipeptide modified bionic nano-vesicle simultaneously presents T7 peptide and RVG peptide through an engineered macrophage membrane, the T7 peptide is combined with a blood-brain barrier transferrin receptor through high affinity to realize efficient brain entry, astrocytes in the brain are specifically recognized by virtue of the RVG peptide, accurate recognition and delivery of target cells in a focus area are realized, and the bipeptide modified bionic nano-vesicle has a good application prospect. Meanwhile, the natural inflammation tropism and immune escape ability of a macrophage membrane are reserved, and the problems that a traditional drug delivery system is low in targeting precision, and cross-barrier distribution and intracerebral distribution are difficult to cooperate are solved.

The invention belongs to the technical field of biomedicine, and particularly relates to a bionic PLGA composite nano-material and a preparation method and application thereof.The bionic PLGA composite nano-material is characterized in that after curcumin and iloumab are co-loaded and packaged through a polylactic acid-glycolic acid copolymer, a bionic membrane is disguised on the outer layer of the polylactic acid-glycolic acid copolymer; and modifying the surface of the biomimetic membrane with phosphatidylated hyaluronic acid. According to the biomimetic PLGA composite nano-material disclosed by the invention, by utilizing the design characteristics of biomimetic membrane and hyaluronic acid modification and combining two anti-atherosclerosis effects of curcumin and iloumab with different mechanisms, the specific aggregation and permeation of a drug at a diseased region are realized, and the bioavailability of a target cell to the drug is improved; the purpose of treating atherosclerosis through synergistic interaction is achieved.

The invention discloses a glucan modified bionic nanoparticle as well as a preparation method and application thereof. Preparing nanoparticles based on PLGA as an inner core; the tumor cell membrane is extracted from B16F10 melanoma cells and is used as an outer capsid; glucan is coupled with a hydrophobic long chain through reductive amination and is modified on the surface of a biomimetic nanoparticle cell membrane through a hydrophobic effect. The dextran nano delivery system is successfully constructed, cell membrane specific antigens and dextran are efficiently and jointly delivered to lymph nodes, tumor cell membranes provide epitopes for immune activation of nanoparticles, and beta-dextran provides molecular support for nanoparticle targeting immune cells. In-vivo and in-vitro activity experiments prove that the glycolipid modified bionic nanoparticles can effectively activate innate immunity through macrophages and NK cells, can promote dendritic cell antigen presentation, and initiate potent adaptive immune response. The bionic nanoparticles have huge development potential of novel tumor vaccines, and a new thought is provided for immunotherapy of cancers.

The invention belongs to but not limited to the technical field of biological medicine, and discloses a target-mediated nucleic acid nano preparation and a preparation method thereof, the target-mediated nucleic acid nano preparation comprises a cationic lipid: C6A1 lipid, a polymer: PLGA, a modified lipid: DSPEPEG2000, and a target: a specific antibody or peptide fragment; a composite structure of cationic lipid and polymer nanoparticles is adopted, a target is modified on the surface of a nano preparation to realize targeted delivery, the target comprises an antibody, a peptide or a small molecule ligand, PEG is modified on the surfaces of the liposome and the nanoparticles, the stability is improved, and the circulation time is prolonged. The targeted nucleic acid nano preparation prepared by the invention can realize efficient and safe nucleic acid and drug delivery.

The invention relates to the technical field of pharmacy, in particular to a saxagliptin oral preparation and a preparation method thereof. The preparation is prepared from the following components in parts by weight: 5 to 15 parts of saxagliptin or salt thereof, 15 to 30 parts of sodium alginate, 10 to 20 parts of chitosan, 10 to 25 parts of polylactic acid-glycolic acid copolymer (PLGA), 5 to 15 parts of polyvinyl alcohol, 3 to 8 parts of N-acetylcysteine, 2 to 6 parts of vitamin E and 1 to 5 parts of sodium lauryl sulfate. According to the present invention, the drug stability is significantly improved, the chitosan and the sodium alginate are subjected to the polyelectrolyte composite reaction to form the stable gel so as to effectively protect the active components of the drug, and the freeze drying treatment ensures the uniform dispersion and the efficient dissolution of the drug, improves the bioavailability, and enables the drug to be rapidly and fully absorbed and utilized; according to the preparation method, the flexibility of the preparation is improved, the preparation can be easily processed into different dosage forms such as tablets and capsules, the requirements of different patients are met, all the components act synergistically, and the medicine performance is jointly improved.