391 results about "PLGA" patented technology

The invention discloses bioactive macroporous hydrogel as well as a preparation method and application thereof. The bioactive macroporous hydrogel comprises hydrogel particles and a bioactive load loaded on the hydrogel particles, the hydrogel particles are obtained by mechanically extruding a hydrogel matrix, and the hydrogel matrix is formed by compounding sodium alginate, agarose and methacrylated hyaluronic acid through hydrogen bond crosslinking and photo-crosslinking; the bioactive load comprises: a) small extracellular vesicles of which the surfaces are modified with macrophage targeted CRV peptides; and b) a PLGA (poly (lactic-co-glycolic acid)) microsphere loaded with a cartilage inducer Kartogenin. The bioactive macroporous hydrogel disclosed by the invention has an internally communicated macroporous network, is beneficial to cell infiltration, blood vessel ingrowth and nutrient substance diffusion, and overcomes the defects that the traditional hydrogel is small in pore size and limits cell behaviors. According to the bioactive macroporous hydrogel disclosed by the invention, dual bioactive loads are adopted to synergistically promote tendon-bone healing, so that the treatment effect is better.

The invention belongs to the technical field of drug delivery, and particularly relates to an M-coated PLGA-10BX compound as well as a preparation method and application thereof. The preparation method comprises the following steps: preparing 10B-enriched boron nitride (h-10BN); extracting a cell membrane of the macrophage RAW264.7; polylactic acid-glycolic acid copolymer (PLGA) nano particles loaded with h-10BN are prepared; and finally, the bionic nano platform M (at) PLGA-10BN based on the macrophage membrane is prepared. The bionic nano-platform prepared by the invention is uniform in particle size and morphology, has relatively high biological safety, and has no obvious damage to various tissues and visceral organs. The compound can be used as a general platform for boron compound delivery, can also be used as an immune activator, is suitable for intravenous injection administration, and expands the application of boron neutron capture therapy (BNCT) in treatment of glioblastoma (GBM).

The invention discloses a ceramic artificial bone material and a preparation method thereof, and belongs to the technical field of ceramic material preparation. The method comprises the following steps: constructing an amphiphilic micelle system through a PEG-PLA (Polyethylene Glycol-Polylactic Acid) block copolymer, firstly preparing a micelle solution, then applying the micelle solution to precursor slurry preparation and PLGA pore-forming agent pretreatment (solving the problems of compatibility and agglomeration) in stages, molding and drying, and then carrying out gradient sintering (removing an auxiliary agent at low temperature and promoting densification at high temperature) to obtain the product. The method solves the problems of poor compatibility of a pore-forming agent and a precursor, powder agglomeration and uneven pore structure in a traditional process, and the obtained material has a three-dimensionally communicated and uniformly distributed micropore structure and is uniform in component.

The invention relates to the technical field of spunlace non-woven materials, and particularly discloses a medical high-performance polylactic acid spunlace composite non-woven material and a preparation method thereof.The preparation method comprises the steps that firstly, polylactic acid fibers and crude natural cellulose fibers are modified, and a compatilizer PLGA-PEG-MDI segmented copolymer is prepared; the preparation process of the spunlace composite non-woven material comprises the steps that viscose is subjected to plasma activation, a compatilizer is sprayed to be loosened and mixed with modified polylactic acid fibers, cross lapping is carried out, polylactic acid added with the compatilizer is melt-blown to the surface of the lapping, and the spunlace composite non-woven material is obtained. And finally, paving the modified crude natural cellulose on the surface of the melt-blown layer to form the spunlace composite non-woven material with good antibacterial property, one-way wet permeability and degradability.

The invention relates to the technical field of drug-loaded microparticles, in particular to a preparation method and application of medical stone / PLGA composite drug-loaded microparticles, medical stone and PLGA are prepared into a structure with activated nano medical stone as a core and a PLGA polymer as a shell through the processes of ball milling, acid activation, emulsification, solvent volatilization and the like, and the medical stone and the PLGA are synergistically matched, so that the drug-loaded microparticles have the advantages that the drug-loaded microparticles are uniform in particle size distribution, and the drug-loaded microparticles have good drug-loaded performance. Firstly, the hydrogel has intelligent responsiveness, can accelerate degradation in alkaline and enzyme environments of wound surfaces, and realizes accurate release of drugs as required; secondly, the long-acting controlled release property is shown, and a dual sustained release barrier is constructed through medical stone adsorption and PLGA wrapping, so that the medicine is released for more than 72 hours at a constant speed following zero-order dynamics, and burst release is effectively avoided. And thirdly, when the drug-loaded particles are used for hydrogel, the drug-loaded particles in nanoscale are combined with the hydration effect of a hydrogel matrix, so that the efficient permeability of the drug in the cuticle is greatly improved.

The invention discloses a bipeptide modified bionic nano-vesicle as well as a preparation method and application thereof, and belongs to the field of biological medicines. The dipeptide modified bionic nano-vesicle comprises nano-particles formed by PLGA (poly (lactic-co-glycolic acid)), and the nano-particles are loaded with a medicine with a nerve protection or nerve repair effect; the surface of the nanoparticle is coated with a macrophage membrane for expressing RVG peptide and T7 peptide. The bipeptide modified bionic nano-vesicle simultaneously presents T7 peptide and RVG peptide through an engineered macrophage membrane, the T7 peptide is combined with a blood-brain barrier transferrin receptor through high affinity to realize efficient brain entry, astrocytes in the brain are specifically recognized by virtue of the RVG peptide, accurate recognition and delivery of target cells in a focus area are realized, and the bipeptide modified bionic nano-vesicle has a good application prospect. Meanwhile, the natural inflammation tropism and immune escape ability of a macrophage membrane are reserved, and the problems that a traditional drug delivery system is low in targeting precision, and cross-barrier distribution and intracerebral distribution are difficult to cooperate are solved.

The invention belongs to the field of medicine, and discloses application of POCM-NP-coated A939572 in preparation of a medicine for treating osteoporosis, the POCM-NP-coated A939572 comprises osteoclast precursor cell membrane vesicles and NP-coated A939572 loaded on the osteoclast precursor cell membrane vesicles, and the NP-coated A939572 is PLGA (poly (lactic-co-glycolic acid)) nanoparticles entrapped with the NP-coated A939572. The POCM-NP-coated A939572 obtained by loading the nanoparticles loaded with the A939572 small molecule medicine into the osteoclast precursor cell membrane vesicles has the effect of relieving the osteoporosis, can inhibit osteoclast differentiation and can be used for preventing, treating and relieving the osteoporosis.

The invention provides vitamin B12 modified microspheres capable of efficiently loading His tag recombinant protein as well as a preparation method and application of the vitamin B12 modified microspheres, and belongs to the technical field of biological medicines. The preparation method of the vitamin B12 modified microsphere for efficiently loading the His tag recombinant protein comprises the following steps: (1) carrying out esterification reaction on unsaturated fatty acid containing sulfydryl, disulfide bond or olefinic bond, saturated fatty acid and vitamin B12 to prepare an amphiphilic vitamin B12-lipid conjugate; and (2) taking the amphiphilic vitamin B12-lipid conjugate, PLGA (poly (lactic-co-glycolic acid)), a multi-sulfydryl cross-linking agent and a photoinitiator as raw materials, and preparing the raw materials into microspheres by adopting a microfluidic method, so as to obtain the vitamin B12 modified microspheres capable of efficiently loading various His tag recombinant proteins. The microsphere provided by the invention overcomes the problems of protein load stability and burst release of traditional microspheres, and various different His tag recombinant growth factors can be flexibly selected according to the requirements on different growth factors in treatment of complex bone defects caused by different pathogenesis.

The invention discloses a special controlled-release nitrogen fertilizer containing flavonoid nutrient signal substances for corn. Comprising 1000 parts of a nitrogen fertilizer, 5-10 parts of flavonoid nutrient signal substance microcapsules and 50-100 parts of a coating material. A flavonoid nutrient signal substance and benzoic acid are dissolved in deionized water, the pH is adjusted to be alkaline, a signal substance solution is obtained, a PLGA solution is slowly poured into the signal substance solution, and a water / oil type emulsion is obtained through homogenization and emulsification; adding a polyvinyl alcohol solution into the water / oil type emulsion in a constant-temperature water bath, homogenizing and emulsifying to obtain a water / oil / water type emulsion; and freezing, centrifuging, washing and freeze-drying the water / oil / water type emulsion to obtain the flavonoid nutrient signal substance microcapsule. Compared with the traditional controlled-release fertilizer, the controlled-release nitrogen fertilizer disclosed by the invention can continuously release signal substances into soil during the growth period of corn, stimulate the growth and metabolism of root systems, promote the colonization of nitrogen-fixing microorganisms and improve the nitrogen fertilizer utilization rate of the corn.

The invention relates to a multistage self-anchoring flexible deep brain electrical stimulation electrode and a preparation method thereof, and relates to the technical field of medical instruments. A bionic root system-octopus whisker composite framework including a main electrode base, a second-stage fractal arm and a third-stage self-anchoring tail end is adopted, intelligent materials such as carbon nano tube / PDMS composite fibers, temperature-sensitive PNIPAAm hydrogel and a degradable PLGA-gelatin composite material are fused, and a mechanical lock catch and biological fusion dual-anchoring mechanism is constructed. The distributed electrode array comprises platinum-iridium alloy, graphene / PDMS and a titanium nitride nano electrode, and cross-scale stimulation from the nuclear group level to the single cell level is achieved. The intelligent regulation and control system solves the problems of brain tissue displacement and chronic inflammation through a pressure feedback degradation and flexible interconnection technology. Compared with a traditional product, the contact area of the electrode is increased by 5-8 times, the stimulation precision reaches the single cell level, and the electrode is suitable for long-term deep brain stimulation treatment of nerve diseases such as Parkinson's disease and epilepsy and has remarkable clinical application value.

The invention relates to the technical field of drug delivery, and discloses a multilayer drug-loaded microsphere for intraocular sustained-release therapy and a preparation process thereof.The multilayer drug-loaded microsphere comprises a core layer, a middle layer and a shell layer, the core layer is composed of a polylactic acid-glycolic acid copolymer (PLGA) and a first hydrophobic drug, the middle layer is formed by wrapping the core layer with carboxymethyl chitosan, and the shell layer is composed of a first hydrophobic drug and a second hydrophobic drug. The first hydrophobic drug comprises a first therapeutic drug, the middle layer comprises a second therapeutic drug, the shell layer is composed of a polylactic acid-caprolactone copolymer PLCL and a third hydrophilic drug, and the first hydrophobic drug, the second therapeutic drug and the third hydrophilic drug are different from one another. Through the layered design of the core layer, the middle layer and the shell layer and in combination with differential degradation rates of PLGA, carboxymethyl chitosan and PLCL materials, gradient release of hydrophobic and hydrophilic drugs is achieved, a drug release curve is matched with the pathological stage of intraocular diseases, macular degeneration rapidly inhibits vascular leakage in the early stage and continuously resists inflammation in the later stage, and the effect of treating macular degeneration is achieved. And the layered slow-release synergistic treatment effect is verified.

The disclosure relates to microparticles and nanoparticles comprising a polymer matrix comprising an uncapped polymer and a net positively charged therapeutic agent at neutral pH. More particularly the disclosure relates to PLGA and / or PLA particles comprising an uncapped polymer for extended, controlled release of positively charged proteins or peptides at neutral pH. Methods of making the particles and administering the particles are also provided.

The invention discloses a compound microorganism freeze-drying protective agent, a preparation method of the compound microorganism freeze-drying protective agent and a use method of the compound microorganism freeze-drying protective agent. Comprising the following raw materials in parts by weight: 0.03 part of disodium ethylene diamine tetraacetate, 5 to 15 parts of trehalose, 2 to 5 parts of sorbitol, 0.5 to 2 parts of ascorbic acid, 0.05 to 0.2 part of N-acetyl-L-cysteine, 0.1 to 0.5 part of polylactic acid-glycolic acid copolymer, 0.01 to 0.05 part of alpha-tocopherol and 1 to 3 parts of bentonite. The PLGA, the alpha-tocopherol and the NAC construct a three-stage synergistic anti-oxidation system from outside to inside: the PLGA is externally blocked, so that rapid consumption of an antioxidant is avoided, and slow release is realized; the alpha-tocopherol realizes oxidative damage repair of cell membranes; nAC maintains the internal reduction environment of cells, reduces oxidative stress and can regenerate alpha-tocopherol at the same time, and reutilization of resources is achieved.

The preparation method comprises the following steps: modifying and connecting IL-15 and PD-1 protein molecules by using biotin, modifying PLGA-PEG by using avidin, and finally preparing the double-targeting nano-micelle through specific binding of the biotin and the avidin and a self-assembly effect of the PLGA-PEG. The dual-targeting nano-micelle IL-15 / PD-1 / PEG-PLGA, which can efficiently target and activate NK cells and target surface high-expression PD-L1 tumor cells, is synthesized and prepared. The mole number of IL-15 and PD-1 loaded on the surface of the dual-targeting nano-micelle is close to 1: 1, and the dual-targeting nano-micelle is regular in form, uniform in dispersion and in the shape of a sphere with the particle size of 254.76 + / -28.02 nm. Meanwhile, it is verified that the dual-targeting nano-micelle IL-15 / PD-1 / PEG-PLGA can effectively improve the recognition and killing efficiency of the NK cells on tumor cells in vivo and in vitro.

The invention describes an extended-release composition comprising PLGA or PLA polymeric microspheres comprising an isoxazoline, preferably sarolaner, and a macrocyclic lactone, preferably moxidectin, and wherein the composition further comprises at least one pharmaceutically acceptable excipient; and uses thereof.

The invention discloses a gradient degradation type personalized absorbable sternum fracture fixing plate and a preparation process, and belongs to the technical field of medical implant materials. Comprising an anatomical adaptive plate body and an integrated locking assembly, the plate body is of a partitioned structure of a middle supporting area and an edge buffering area, a gradient pore array penetrating through the thickness direction is formed in the surface of the plate body, and a hydroxyapatite-collagen composite coating is loaded on the inner wall of a pore; the plate body is made of a composite absorbable material through 3D printing forming and hot pressing post-treatment, and the composite absorbable material comprises, by weight, 60%-70% of polylactic acid-glycolic acid copolymer (PLGA, LA / GA = 75 / 25), 15%-20% of silanized magnesium alloy microwires, 5%-10% of hydroxyapatite nano-particles and 5%-10% of polycaprolactone (PCL). Space-time matching of the degradation rate and the bone healing process is achieved through the partition gradient structure, the magnesium alloy microwires enhance the mechanical property and release active ions to promote osteogenesis, and the method has the advantages of personalized adaptation, gradient degradation, bone regeneration promotion and the like.

The invention discloses an MKP7 targeted bionic nanoparticle preparation as well as a preparation method and application thereof. The MKP7 targeted bionic nanoparticle preparation is a bionic nanoparticle siMKP7 (at) NP-M-pHLIP, wherein the bionic nanoparticle siMKP7 (at) NP-M-pHLIP is coated with a macrophage membrane modified by pHLIP and is loaded with siMKP7. According to the preparation, a PLGA-PEI copolymer is used as a carrier core to wrap siMKP7, an outer layer is coated with a macrophage membrane, the surface of the membrane is modified with pHLIP targeting peptide, and spherical nanoparticles with bionic camouflage and pH sensitive targeting delivery functions are constructed. In-vitro experiments show that the nanoparticles can be effectively ingested by macrophages, and macrophage lipid deposition induced by ox-LDL is remarkably inhibited; in-vivo experiments prove that the preparation can be delivered to atherosclerotic plaque parts in a targeted manner, and by inhibiting expression of MKP7 in macrophages, the carotid plaque area is effectively reduced, the serum inflammatory factor level is reduced, and the blood lipid metabolism is regulated, so that development of atherosclerosis is inhibited, and the curative effect of atherosclerosis is improved. A high-efficiency and low-toxicity nucleic acid drug delivery system is provided for treatment of atherosclerosis.

The invention relates to a degradable nerve repair membrane and a preparation method thereof, and belongs to the technical field of nerve repair membranes, the degradable nerve repair membrane comprises a biological composite structure inner layer, a support outer layer and a bonding layer, and the biological composite structure inner layer and the support outer layer are bonded through the bonding layer. According to the invention, polypyrrole with excellent electrochemical activity, environmental stability, controllable conductivity and poor mechanical properties and collagen nanofibers with high biocompatibility and capable of forming a nano-scale fiber network are adopted to form a conductive-biological composite structure as an inner layer through electrostatic spinning, and the PLGA porous membrane is prepared through 3D printing. A PLGA porous membrane with parallel axial microchannels on the surface is used as an outer layer, so that the membrane body structure can effectively guide directional growth of axons while ensuring conductivity, accurate preparation can be realized according to needs, the degradation period is matched with the nerve regeneration rate, and the degradation time can be regulated and controlled through the proportion of a copolymer; the repairing effect is prevented from being influenced by too fast or too slow degradation.

The invention relates to the technical field of biological medicines, in particular to an anti-tumor compound as well as a preparation method and application thereof. The nano-diamond cannot effectively kill tumor cells due to good biocompatibility, and the ferrocene with an aromatic ring structure is combined with the nano-diamond, so that a good anti-tumor effect can be fully exerted. The TAT-PEG-PLGA has the advantages that the TAT-PEG-PLGA is high in membrane penetrating capability, water solubility, biocompatibility, stability and targeting property; the TAT-PEG-PLGA with adsorption force on the surface is used for loading the ferrocene-nano-diamond and the adriamycin to obtain the anti-tumor compound, so that the ferrocene-nano-diamond and the adriamycin can effectively enter tumor cells to jointly play an anti-tumor effect. The obtained anti-tumor compound can effectively inhibit the activity of melanoma cells A375, is an effective tumor inhibitor, and can provide a new treatment strategy and choice for tumor treatment.

The invention discloses difunctional nano drug-loaded eye drops for xerophthalmia and a preparation method of the difunctional nano drug-loaded eye drops, and belongs to the field of pharmaceutical preparation technologies and ophthalmology pharmacy. The core of the invention is to construct a composite nanoparticle which has a core-shell structure and has long-acting adhesion lubrication and targeted control drug release functions. The preparation method comprises the following steps: firstly, encapsulating a hydrophobic immunosuppressant such as cyclosporine A in a biodegradable pad polymer matrix such as polylactic acid-glycolic acid copolymer (PLGA) by an emulsification-solvent evaporation method to form a drug-loaded polymer nano core; through the exquisite integrated design of the structure and the function, collaborative targeted therapy of two core pathological links of'tear film instability 'and'immune inflammation' of xerophthalmia is achieved, the bioavailability of the medicine is remarkably improved, the medication frequency and eye irritation are reduced, and the curative effect of xerophthalmia is improved. A more efficient and safer administration scheme with better patient compliance is provided for clinical treatment of xerophthalmia.

The application discloses an STM2457-PLGA nano drug, an artificial lens and a preparation method and application thereof, and relates to the technical field of biological medicine. The preparation method of the STM2457-PLGA nano drug comprises the following steps: preparing nano microspheres by injecting an oil phase comprising STM2457 and PLGA and a water phase comprising PVA into a microfluidic chip, and obtaining the STM2457-PLGA nano drug after freeze-drying of the nano microspheres, wherein the particle size of the STM2457-PLGA nano drug is 50-300 nm. The long-acting release of STM2457 carried by the surface-modified artificial lens is realized through the controlled release effect of the PLGA nano microspheres, and the slow-release period can cover the high-incidence period of postoperative complications of cataract surgery through process optimization, far exceeding the traditional short-term release system, and can continuously meet the long-term prevention and treatment needs after surgery, and effectively reduce the risk of complications such as after-cataract.

The invention discloses a hydrogel composition for three-dimensional culture of tumor cells and a preparation method of the hydrogel composition. The hydrogel composition is prepared from poly (lactic acid-glycolic acid) copolymer-polyethylene glycol-poly (lactic acid-glycolic acid) copolymer PLGA-PEG-PLGA and methylacryloyl gelatin GelMA, the mass ratio of the poly (lactic acid-glycolic acid) copolymer-polyethylene glycol-poly (lactic acid-glycolic acid) copolymer PLGA-PEG-PLGA to the methylacryloyl gelatin GelMA is (2-5): 10. According to the hydrogel composition, methacrylated gelatin GelMA is used as a main substrate, so that good biocompatibility and cell adhesion are provided; according to the invention, polylactic acid-glycolic acid copolymer-polyethylene glycol-polylactic acid-glycolic acid copolymer PLGA-PEG-PLGA is used as an auxiliary component, lactic acid is generated through biodegradation of the polylactic acid-glycolic acid copolymer-polyethylene glycol-polylactic acid-glycolic acid copolymer-PEG-PLGA, the pH of a microenvironment is dynamically reduced to 6.5-6.9, tumor acidic conditions are simulated, and pores formed after degradation can provide more growth and invasion spaces for tumor cells.

The present application relates to microspheres having a physiologically active substance uniformly dispersed therein and sustained-release preparations containing the same. In the present application, microspheres are provided, which are microspheres having a physiologically active substance uniformly dispersed therein with a lactic acid / glycolic acid copolymer (PLGA) as a main component, characterized in that the average volume-based particle diameter of the microspheres is 1 μm or more and 150 μm or less, and no lump or pore of the physiologically active substance of 1.5 μm or more is present in the microspheres. The microspheres of the present application can appropriately control the initial release amount of the physiologically active substance and the release rate during the period thereafter, and continuously release the physiologically active substance in vivo for a certain period.

The invention belongs to the technical field of medical biological dressings, and provides a composite dressing with oxygen release and insulin delivery functions and a preparation method thereof.The composite dressing comprises a supporting base layer, a function slow release layer and a protective film layer which are sequentially compounded from bottom to top; the supporting base layer is a medical porous polyurethane film modified by plasma; the functional sustained release layer is composed of hyaluronic acid-chitosan composite gel, oxygen release microspheres and insulin liposome; the protective film layer is a polyethylene terephthalate film of which the surface is provided with a sawtooth edge easy to tear; through an integrated three-layer patch structure, by combining the synergistic effect of PLGA microsphere controlled-release oxygen, liposome low-molecular chitosan insulin delivery and gel matrix, the skin injury microenvironment is effectively improved, inflammation is inhibited, and the healing time is shortened.

The invention provides special gel for medical surgical site ice compress and a preparation method thereof, and relates to the technical field of medical treatment. The preparation raw materials at least comprise: a main polymer: polyvinyl alcohol (PVA), bacterial nanocellulose (BNC), and poly N-isopropylacrylamide; the functional additives comprise double modified starch (OSA + phosphate), nano-silver (the particle size is 20-50 nm), a thrombin-fibrinogen compound and EGF / bFGF microspheres (PLGA); and the auxiliary components comprise glycerol, citric acid and purified water. According to the multifunctional ice compress gel, a structure which is loose outside and tight inside is formed through freeze drying, the cold compress uniformity and the air circulation performance are improved, the double modified starch and the cross-linking agent cooperate, the degradation period is prolonged to 72 h, the replacement frequency is reduced, the multifunctional ice compress gel has the functions of physical cooling, hemostasis repair, antisepsis and anti-inflammation, the cooperative requirement of postoperative cold compress and wound surface nursing is met, and the multifunctional ice compress gel is worthy of popularization and application. The device is suitable for postoperative incision cold compress, burn / acute stage cooling and hemostasis of wounds and auxiliary repair of chronic wounds.

The application discloses a kind of heat insulation coating based on phase change material and its preparation method and application, belong to functional coating technical field.The coating includes A component and B component, A component includes water-based resin, film-forming aid, antifreezing agent and deionized water;B component includes self-repairing elastic phase change microcapsule, pigment, heat insulation composite filler and functional aid.The self-repairing elastic microcapsule adopts PLGA and polycaprolactone diol composite shell, core material uses phase change material and self-repairing resin, the heat insulation composite filler includes boron nitride, nanometer silicon dioxide and montmorillonite, the coating prepared simultaneously has excellent heat insulation performance, self-repairing function, interface bonding strength, mechanical property and storage stability, can be widely applied in building energy saving, industrial equipment protection, traffic vehicle and electronic appliance etc.

The invention discloses a dog head roundworm rTcMUCs-PLGA nano vaccine as well as a preparation method and application thereof, and the dog head roundworm rTcMUCs-PLGA nano vaccine is characterized in that the nano vaccine takes PLGA nano microspheres as a carrier and is loaded with TcMUC-2 and / or TcMUC-3 recombinant protein. Animal protection test results show that compared with a PBS and PLGA control group, the number of L3-stage larvae of the dog head roundworm in livers and lungs of mice inoculated with the rTcMUC-2-PLGA and the rTcMUC-3-PLGA in an immune group is obviously reduced, the number of L3-stage larvae of the dog head roundworm in the immune group is 55.31% and 53.61% compared with the number of L3-stage larvae of mice inoculated with the rTcMUC-2 and the number of L3-stage larvae of the mice inoculated with the rTcMUC-3 in an immune group taking saponin as an adjuvant, and after the rTcMUC-2 and the rTcMUC-3 are coated with the PLGA as a nano-carrier, the L3-stage larvae of the dog head Compared with the prior art, the PLGA nano-vaccine has the advantages that the immune frequency is reduced from three times to two times, the worm reduction rates respectively reach 65.13% and 55.9%, and the result proves that the PLGA nano-vaccine can be used as an adjuvant of recombinant protein to reduce the immune cycle and induce a host to generate better immune protection force to inhibit invasion and migration of larvae of the dog head roundworm.

Disclosed herein is a polysialic acid (PSA)-polymer conjugated compound represented by Structural Formula (I): or a pharmaceutically acceptable salt thereof, wherein P is a poly (lactide-co-glycolide)-poly (ethylene glycol) copolymer (PLGA-PEG) and p is an integer from 4 to 200; nanoparticles comprising the same; and methods of treating ophthalmic diseases using the same. (I)

The invention relates to the technical field of nano-micelles, in particular to rifapentine-loaded bone targeting nano-micelles as well as a preparation method and application thereof.The rifapentine-loaded bone targeting nano-micelles are prepared by accurately controlling a synthesis process (shading, catalyst ratio, temperature and feed ratio) of a bone targeting nano-micelle carrier (ALN-PLGA-mPEG) modified by alendronate sodium (ALN). The bone targeting ability of alendronate sodium (ALN) is combined with the drug loading and long circulation advantages of polymer mPEG-PLGA micelles to construct a bone targeting nano-micelle carrier (ALN-PLGA-mPEG), so that the obtained rifapentine-loaded bone targeting nano-micelle (ALN-PLGA-mPEG (at) RPT) can significantly improve the bone targeting and slow release performance, and provides a new strategy with high efficiency and low toxicity for precise treatment of bone tuberculosis.

The utility model provides a fibrous membrane for guiding periodontal tissue regeneration, and relates to the technical field of medical instruments. Preparing a PLGA double-layer fiber membrane loaded with drug-loaded sodium hyaluronate particles through the cooperative work of electrostatic spinning and electrostatic spraying; the sodium hyaluronate particles can greatly improve the loading rate of hydrophilic anti-inflammatory antibacterial and bone regeneration promoting medicines; a PLGA compact layer in the PLGA double-layer fiber membrane is loaded with first sodium hyaluronate particles loaded with anti-inflammatory and antibacterial drugs, and when the first sodium hyaluronate particles make contact with gingival tissue, cell adhesion and permeation can be hindered, and the anti-inflammatory and antibacterial effects can be achieved; a PLGA loose layer in the PLGA double-layer fiber membrane carries second sodium hyaluronate particles loaded with a bone regeneration promoting medicine, and the second sodium hyaluronate particles can promote osteoblast adhesion and facilitate dentale regeneration when making contact with bone tissue.