Composite membrane for guiding bone tissue regeneration and preparation method thereof

A technology of bone tissue regeneration and composite membrane, applied in coating, medical science, surgery, etc., can solve the problems of unsatisfactory clinical effect, increased chance of trauma, insufficient biological activity, etc., to avoid unstable interface bonding and excellent mechanical properties. Performance, the effect of excellent biological activity

Inactive Publication Date: 2013-05-08
AFFILIATED STOMATOLOGICAL HOSPITAL OF NANJING MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the late 1970s and early 1980s, Nyman, Gottlow and Becket et al successively conducted research on non-degradable materials such as polyacetal, polytetrafluoroethylene (PTFE), and silicone membranes. Biocompatibility, but because it cannot be absorbed by the tissue, a second operation is required to remove the material, which increases the chance of trauma, is diffi

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] A composite film for guiding bone tissue regeneration, comprising a base layer, an intermediate modified layer and a functionalized surface layer; the base layer is a PLGA film, and the intermediate modified layer is polydopamine formed by polymerization of dopamine and adsorbed to the PLGA film Surface formation, the functionalized surface layer is formed by the reaction of rat tail tendon type I collagen and collagen / chitosan mixture with active groups in polydopamine, and the thickness of the PLGA film is 100 microns.

[0026] The preparation method of this kind composite film, its preparation step comprises as follows:

[0027] (1) Weigh PLGA and dissolve it in chloroform to obtain a PLGA chloroform solution with a concentration of 1g / 10ml;

[0028] (2) Pour the PLGA chloroform solution prepared in step (1) into a polytetrafluoroethylene container, quickly transfer it to a laminar flow cabinet at 4°C, take it out after 10 hours, place it at room temperature for 72 h...

Embodiment 2

[0036] A composite film for guiding bone tissue regeneration, comprising a base layer, an intermediate modified layer and a functionalized surface layer; the base layer is a PLGA film, and the intermediate modified layer is polydopamine formed by polymerization of dopamine and adsorbed to the PLGA film Surface formation, the functionalized surface layer is formed by the reaction of type I collagen and collagen / chitosan mixture with active groups in polydopamine, and the thickness of the PLGA film is 300 microns.

[0037] The preparation method of this kind composite film, its preparation step comprises as follows:

[0038] (1) Weigh PLGA and dissolve it in chloroform to obtain a PLGA chloroform solution with a concentration of 1g / 20ml;

[0039](2) Pour the PLGA chloroform solution prepared in step (1) into a polytetrafluoroethylene container, quickly transfer it to a laminar flow cabinet at 4°C, take it out after 8 hours, place it at room temperature for 24 hours, and then tra...

Embodiment 3

[0047] A composite film for guiding bone tissue regeneration, comprising a base layer, an intermediate modified layer and a functionalized surface layer; the base layer is a PLGA film, and the intermediate modified layer is polydopamine formed by polymerization of dopamine and adsorbed to the PLGA film On the surface, the functionalized surface layer is formed by the reaction of rat tail tendon type I collagen and collagen / chitosan mixture with active groups in polydopamine, and the thickness of the PLGA film is 200 microns.

[0048] The preparation method of this composite film, its preparation step comprises as follows:

[0049] (1) Weigh PLGA and dissolve it in chloroform to obtain a PLGA chloroform solution with a concentration of 1g / 15ml;

[0050] (2) Pour the PLGA chloroform solution prepared in step (1) into a polytetrafluoroethylene container, quickly transfer it to a laminar flow cabinet at 4°C, take it out after 9 hours, place it at room temperature for 48 hours, and...

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PUM

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Abstract

The invention discloses a composite membrane for guiding bone tissue regeneration and a preparation method thereof. The composite membrane comprises a basal layer, an intermediate modified layer and a functional surface layer, wherein the basal layer is a poly lactic-co-glycolic acid (PLGA) membrane, the intermediate modified layer is formed by polymerizing dopamine to form polydopamine and adsorbing the polydopamine to the surface of the PLGA membrane, and the functional surface layer is formed by reaction of I-type collagen or a collagen/ chitosan mixture and active groups in the polydopamine. The preparation method for the composite membrane comprises a series of steps of membrane preparation, rinsing, airing and the like. The artificially synthesized macromolecular polymer is used as a basal material, the surface of the membrane obtained after the basal material is grafted with a natural polymer to undergo surface function activation and functional modification has a nano structure and active groups which are not owned by the original synthesized polymer, the affinity of osteoblasts is improved, and adhesion and proliferation of cells are promoted; and by using the synthesized macromolecular polymer as the base, high mechanical strength and controllable degradability are provided, and the grafted natural polymer is combined with the base firmly by pre-modification of the surface of the synthesized macromolecular polymer.

Description

technical field [0001] The invention relates to the field of polymer biomedical materials, in particular to a surface-functionalized composite film for guiding bone tissue regeneration and a preparation method thereof. Background technique [0002] The concept of guided tissue regeneration (guided tissue regeneration, GTR) was first proposed by Nyman et al. It refers to relying on mechanical barriers to selectively guide cells to attach and proliferate to damaged parts to achieve the purpose of tissue repair. Guided tissue regeneration is a theory and technology to promote tissue regenerative healing developed on the basis of in vitro, in vivo and clinical experimental research in the 1980s. Therefore, as a new material with the function of guiding tissue regeneration, the medical guided tissue regeneration material will play a great role in clinical medicine, and its development and research are attracting great attention from the material and medical circles. [0003] Me...

Claims

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Application Information

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IPC IPC(8): A61L31/12A61L31/06A61L31/04A61L31/14C08J7/12C08J7/04C08J5/18C08L67/04
Inventor 章非敏夏阳陈刚顾宁
Owner AFFILIATED STOMATOLOGICAL HOSPITAL OF NANJING MEDICAL UNIV
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