43 results about "Functional modification" patented technology

The invention provides a preparation method of a substrate of an external vesicle functional marker rapid detection kit, and belongs to the technical field of external vesicle functional marker detection.The preparation method comprises the steps that a high-purity coating working solution is prepared, and precise spraying is achieved through a piezoelectric type ink jet system; substrate functional modification is completed through confining liquid treatment and labeled antibody working solution preparation, a double-layer game model containing detection sensitivity optimization and process stability optimization is established, and an optimal parameter combination is obtained through game iterative calculation by combining the synergistic effect of a quality evaluation function and a stability control function. A characteristic parameter database is established to store historical production data and quality detection results, a multi-parameter joint evaluation method is adopted to calculate a comprehensive score and carry out unified product grading, and a quality control system and a standardized parameter optimization mechanism are driven through full-process data. The technical problem that the quality consistency difference is large in the batch production process is solved.

The application provides a preparation method of a dot array biochip. The application forms a nano-pore dot array on a chip substrate through nano-imprinting, uniformly forms a functional modification layer on the dot array surface through a hydrogel, and then performs etching treatment on the substrate by using a deflected ion beam; an angle between an ion beam incident direction and a surface of a slide stage is θ during etching, so as to perform selective etching on the functional modification layer; through the etching with the angle, the functional modification layer of the bottom and part of the sidewall of the nano-pore can be reserved, and the functional modification layer of other parts is etched clean by the ion beam; the ion source of the IBE is argon, which is stable in chemical property and has no obvious influence on the chemical composition of the material, so that the damage of chemical residues to the performance of the functional material is avoided; the IBE does not produce chemical reaction byproducts, so that the risk of surface residual particles and pollution is reduced; the ion beam energy of the IBE is adjustable, the relatively mild etching avoids damage to the substrate, and the influence on the lattice and surface state of the surface layer material is small.

The invention discloses a method for synthesizing millions of unique DNA tags through primer combination and application of the method, and relates to the field of molecular biology. According to the method, partial complementary pairing characteristics of F, X and Y primers are utilized, and under the synergistic effect of T4 DNA polymerase and ligase, millions of unique DNA tags are generated through one-time reaction through annealing-filling-ligation three-step reaction. The label structure comprises 5'end functional modification sites (such as amino and biotin) and a customizable sequencing joint, and is suitable for high-throughput labeling scenes such as single-cell multiomics, space proteomics and antibody coupling (AOC). Compared with a traditional one-by-one synthesis method, the method has the advantages that the single-tag synthesis cost is reduced by 80% or above, a reaction system is compatible with automatic operation, the tag combination complexity can reach 5.6 * 10 (based on 384 * 384 * 384 primer combination), and an efficient solution is provided for large-scale molecular marking.

The invention discloses an up-conversion nano-composite with a core-shell structure, a preparation method and application, and belongs to the technical field of biomedical bimodal nano-probes. According to the nanocomposite, up-conversion nanoparticles (UCNPs) serve as a core, metal-organic frameworks (MOFs) serve as a shell layer, horse radish peroxidase (HRP) is loaded on the shell layer, and UCNP (at) MOF / HRP is formed. Subsequent biological functional modification, such as coupling of an antibody, an antigen, nucleic acid and other substances, can be carried by an activating group or a metal coordination bond carried by a shell ligand of the upconversion nanocomposite MOF with the core-shell structure, so that the functions of specific recognition, targeted capture, specific loading and the like are realized.

The invention provides an iron-based nano material, a preparation method and application, the material comprises magnetic iron oxide nano particles and a functional modification layer on the surface, the magnetic iron oxide nano particles are Fe3O4, the particle size is 10-30 nm, and the magnetic iron oxide nano particles have specific oxygen vacancy concentration, 337 nm laser absorptivity and saturation magnetization; the functional modification layer is an amino-containing polymer layer, such as a polydopamine or cysteine-polyethyleneimine copolymer, and can reduce salt ion interference. The material solves the problems of low molecular weight region background interference, non-uniform crystallization, low ionization efficiency, poor salt tolerance and the like during detection of 100-400Da small molecular metabolites in trace samples such as tears and the like by optimizing magnetic responsiveness, photo-thermal conversion capability and salt resistance of an existing matrix; the method realizes high sensitivity and high repeatability (detection), and is suitable for metabonomics analysis of trace biological samples.

The invention discloses a special membrane material for targeted trapping of macrobrachium larvae in water and a preparation method of the special membrane material, and belongs to the field of water environment biological pollution prevention and control. The membrane material is of a multi-layer composite structure and comprises a nanofiber membrane base material layer, an interface adhesion layer and a functional modification layer. The interface adhesion layer is bionic coacervate gel formed by curing hydrophobic polyelectrolyte and phytic acid; the functional modification layer is covalently grafted with a bionic adhesion protein active peptide fragment and a macrobrachium larva chemical pheromone. The material actively attracts larvae to approach through chemical pheromones, high-strength adhesion capture is achieved through bionic peptide fragments rich in aromatic and cationic amino acids, and trapping is integrated. The preparation method comprises the steps of electrostatic spinning of a base material, surface activation, gel coating, functional modification and the like. The material disclosed by the invention has high selectivity and high capture rate on the larvae of the Limnoperna fortunei, is environment-friendly, long-acting and stable, and is suitable for green prevention and treatment of fouling of the larvae of the Limnoperna fortunei in facilities such as water pipelines and pump stations.

The invention discloses a protein-based multifunctional food preservative film as well as a preparation method and application thereof, and belongs to the technical field of functional modification of natural polymer materials. The preparation method of the protein-based multifunctional food preservative film comprises the following steps: firstly, carrying out hydrolysis pretreatment on zein by utilizing the hydrolysis effect of protease, then grafting catechol into a protein structure by utilizing an acyl transfer reaction of TGase, and finally, carrying out functional grafting modification on the zein by utilizing chitosan by utilizing an oxidation reaction of laccase, so as to obtain the protein-based multifunctional food preservative film. The physical and mechanical properties, the stability and the antibacterial activity of the zein film are further improved, and the protein film is endowed with antioxidant activity, so that the protein-based multifunctional food preservative film material suitable for food preservation is prepared. When the protein-based multifunctional food fresh-keeping film is used for fruit fresh-keeping treatment, the shelf life is remarkably prolonged, and the protein-based multifunctional food fresh-keeping film has a good fresh-keeping effect.

The present application belongs to the technical field of extracellular vesicle separation and analysis, and particularly relates to a portable extracellular vesicle separation and analysis system with bubble micro-group self-driven scanning, which comprises a polytetrafluoroethylene micro-flow tube, a dyeing agent, a bubble robot capture carrier with surface antibody functional modification, and a detection module. During operation, the bubble robot is driven by buoyancy to perform reciprocating scanning movement along the axial direction of the tube by tilting the micro-flow tube, so as to actively and efficiently capture target extracellular vesicles in the sample. After capture, the bubble robot is detected by using a fluorescently labeled ssDNA dyeing agent, and the bubble robot transferred to the observation carrier is subjected to fluorescent signal analysis by the detection module. The system integrates new materials and micro-flow control technology, realizes rapid and portable separation and quantitative analysis of extracellular vesicles, solves the problems of existing extracellular vesicle separation technology, such as complicated operation, dependence on large equipment and difficulty in realizing rapid on-site detection, and is particularly suitable for on-site detection of clinical samples.

The present application relates to the technical field of genetic engineering, and particularly provides a Diels-Alder reaction enzyme mutant as well as a preparation method and application thereof. The present application relates to functional modification and strengthening of a Diels-Alder (D-A) reaction enzyme MaDA1 derived from mulberry, so that the enzyme catalyzes intermolecular D-A reaction of a non-natural dienophile containing a ''handle group'' (carboxyl, ester or a precursor thereof) and various dienophiles, so that further modification of the enzymatic D-A product can be realized through decarboxylation functionalization reaction, and diversification and stereoselective synthesis of a cyclohexene skeleton structure are realized.

The application relates to a kind of lipid nanocarriers coupled with GO-203 and loaded with DMAMCL and its preparation method and application, belong to the field of biological medicine and nano-preparation.The lipid nanocarriers include water phase core, lipid bilayer and surface modification layer from inside to outside, wherein water-soluble dimethylamine methyl chloride (DMAMCL) is loaded in the water phase core;The lipid bilayer is wrapped in the water phase core, and coumarin-6 is embedded as fluorescent marker for tracing and detection;Functional modification layer is arranged on the outer surface of the lipid bilayer, including DSPE-PEG2000-COOH and DSPE-PEG2000-GO-203, wherein GO-203 is cell membrane penetrating peptide.The lipid nanocarrier provided by the application can realize the synergistic delivery of GO-203 and DMAMCL, be used for regulating tumor immune microenvironment and be used for the treatment of triple-negative breast cancer.

The present invention discloses an adsorbent for removing excess hepcidin and a preparation method thereof. The adsorbent for removing excess hepcidin is made by using EDTA as a cross-linking agent to form cross-linked chitosan microspheres embedded with zirconium phosphate and / or zirconium oxide, and L-glutathione is bonded to the surface of the cross-linked chitosan microspheres. The adsorbent for removing excess hepcidin of the present invention uses chitosan microspheres as a carrier, and while the microspheres are cross-linked, further bonding ligands are introduced, eliminating the need for additional cross-linking agents and ligands. The reaction conditions are mild, the preparation method is simple, and the cross-linked microspheres have suspended carboxyl groups, which can be further coupled with peptides and amino acids to achieve functional modification. The microspheres can withstand high-temperature steam sterilization and effectively remove excess hepcidin and urea nitrogen from the blood.

The invention belongs to the field of food science and microbial engineering, and particularly relates to fusarium venanii protein, low-lipoprotein food as well as a preparation method and application of the fusarium venanii protein and low-lipoprotein food. The invention provides a fusarium venanii TB01 protein with high emulsibility as well as a preparation method and application of the fusarium venanii TB01 protein. According to the method, fusarium venanii TB01 mycelium is adopted as a raw material, composite enzymolysis and ultrasonic treatment are innovatively and sequentially cooperated, and functional modification of a protein structure is achieved while high-content protein (larger than or equal to 82%) is extracted through efficient wall breaking. Tests show that the two key indexes of emulsifying activity and stability of the obtained protein are obviously superior to those of soybean protein, and the emulsifying stability of the obtained protein is superior to that of commercial whey protein. Therefore, the protein obtained by the invention can be used as a high-performance emulsifying ingredient, is used for replacing or partially replacing whey protein or soybean protein, and is applied to food systems such as beverages, plant yoghurt, sauce and the like.

The invention provides a bacterial outer membrane vesicle capable of efficiently targeting tumors and a preparation method of the bacterial outer membrane vesicle, and relates to the technical field of biological medicines. The target protein PDL1 binding peptide is successfully modified on the surface of the bacterial outer membrane vesicles to prepare the bacterial outer membrane vesicles PDL1-OMVs, the particle size range of the bacterial outer membrane vesicles PDL1-OMVs is 20-250 nm, and the bacterial outer membrane vesicles PDL1-OMVs have an obvious saucer-like double-layer membrane structure and accord with the structural characteristics of the bacterial outer membrane vesicles. According to the PDL1-OMVs provided by the invention, on one hand, the defect that the PD-L1 binding peptide is weak in targeting efficacy is improved, the targeting capability of the PD-L1 binding peptide is remarkably improved, on the other hand, targeting function transformation is realized on the bacterial outer membrane vesicles, and the PDL1-OMVs can realize a specific targeting effect on tumor cells, so that conditions are provided for the PDL1-OMVs serving as a carrier for targeted delivery of tumor drugs.

The application provides an electroluminescence-enhanced biosensor and a preparation method thereof. The electroluminescence-enhanced biosensor comprises, from bottom to top, a flexible substrate, a coplanar electrode layer, a mixed luminescence layer, a functional modification layer and a biological recognition layer which are sequentially stacked; the coplanar electrode layer is formed by forming a pair of parallel electrodes on the flexible substrate with a conductive material; the mixed luminescence layer is formed by uniformly coating an alternating current electroluminescence material on the coplanar electrode layer; the functional modification layer comprises an adhesion layer arranged on the mixed luminescence layer and a material layer arranged on the adhesion layer; the adhesion layer is made of a positively charged biocompatible polymer, and the material layer is made of a two-dimensional material containing a phosphate group; the biological recognition layer is formed by fixing specific antibodies on the material layer and then sealing unreacted sites with a sealing material; after a sample to be measured is added to the biological recognition layer, an alternating current voltage is applied, and a detection signal is read.

The present application belongs to the field of food science and microbial engineering, and specifically relates to a fusarium venenatum protein, a low-fat protein food and a preparation method and use thereof. The present application provides a high-emulsifying fusarium venenatum TB01 protein, a preparation method and application thereof. The present application uses fusarium venenatum TB01 mycelium as raw material, and innovatively realizes the functional modification of protein structure by sequentially cooperating complex enzymolysis and ultrasonic treatment, thereby efficiently breaking the cell wall to extract high-content protein (≥82%) and realizing the functional modification of protein structure. Tests show that the emulsification activity and stability of the obtained protein are significantly better than those of soybean protein, and the emulsification stability is better than that of commercial whey protein. Therefore, the protein obtained by the present application can be used as a high-performance emulsifying ingredient to replace or partially replace whey protein or soybean protein, and is applied to beverage, plant yogurt, sauce and other food systems.

The invention discloses a fertilizer synergist based on marine biogen functional group modification and a preparation method and application thereof, and relates to the technical field of agricultural biotechnology and novel fertilizer additives, and the preparation method comprises the following steps: raw material pretreatment: taking marine algae, shellfish or marine fish processing leftovers as raw materials, cleaning, crushing to 60-100 meshes, and drying to obtain the fertilizer synergist; according to the method, marine biogen is taken as a raw material to obtain marine biogen coarse powder, active functional groups such as sulfonic groups and carboxyl groups are successfully introduced to a molecular skeleton of the marine biogen through alkaline hydrolysis and a specific functional group directional modification process, and a final product has high cation exchange capacity and pH buffer characteristics based on Hexchange and neutralization reaction through ion exchange treatment; the synergist can adsorb and preserve nutrient ions, and after the synergist is applied to soil, through interaction of functional groups of the synergist and ions of a rhizosphere environment, the pH of a micro-domain can be easily adjusted to be neutral, so that favorable conditions are created for effective utilization of nutrients, and meanwhile resource utilization of marine processing leftovers is achieved.

The invention discloses a fluorescence analysis and detection method and system based on quantum dots, and belongs to the technical field of analysis and detection. According to the method, functional modified quantum dots are used as fluorescent probes, a fluorescence detection system is constructed through specific recognition reaction, and high-sensitivity and high-specificity detection of a target detection object is realized by combining collection and quantitative analysis of fluorescence signals. The corresponding system comprises an excitation light source module, a sample reaction module, a fluorescence detection module, a data processing module and a control module, all the modules work cooperatively, key parameters in the detection process can be precisely regulated and controlled, and the detection precision and stability are improved. The method solves the problems of poor probe stability, low quantum yield and high detection limit in a traditional fluorescence detection method, has the advantages of high detection speed, high specificity and wide application range, and can be widely applied to the fields of food safety detection, environmental pollutant monitoring, biomedical diagnosis and the like.

The invention discloses magnetic biochar as well as a preparation method and application thereof. The preparation method comprises the following steps: calcining agricultural wastes containing organic matters to obtain a biochar initial material; soaking the biochar initial material in a mixed solution containing an iron source, a reducing agent and an alkalinity regulator; and then carrying out hydrothermal reaction. The biochar precursor is prepared from agricultural wastes containing organic matters, such as comprehensive solid particles in aquaculture tail water, and complex components and original characteristics of the biochar precursor are reserved to the greatest extent. Meanwhile, the biochar is subjected to magnetic functional modification, the dual advantages of rapid adsorption and convenient recovery are achieved, and the problem that conventional biochar is difficult to separate, recover and reuse from the water body is solved. According to the method, resource utilization of solid particles in the breeding tail water can be achieved, meanwhile, antibiotic pollutants can be effectively removed, and the spreading risk of drug-resistant bacteria is reduced.

The invention discloses a nano-microsphere time-resolved fluorescent probe as well as a preparation method and application thereof. The preparation method comprises the following three steps: preparation of core-layer nano microspheres, shell-layer functional modification and fluorescence enhancement and stabilization treatment. A polylactic acid-glycolic acid copolymer (PLGA) is used as a core-layer carrier to load a rare earth ion complex, and a SiO2 coating layer and a targeting ligand are combined for modification, so that the nano-microsphere fluorescent probe with a core-shell structure is prepared. The probe has the characteristics of long fluorescence lifetime (5-8ms), high quantum yield (greater than or equal to 35%), strong stability, excellent targeting property and the like, and can be widely applied to food safety detection (such as quinolone antibiotics and microcystin MC-LR) and environmental pollution detection (such as heavy metal ions such as Hg < 2 + > and Pb < 2 + >), and the detection limit is as low as 0.05 ng / mL or 0.1 nmol / L. The problems that a traditional fluorescent probe is low in loading capacity, poor in stability and insufficient in targeting property are solved, and the method has remarkable industrial application value.

The application provides a kind of extracellular vesicle quantitative detection method based on self-interference spectrum reconstruction, it is related to the intersection field of biomedical detection and optical engineering.The method comprises: adding clinical sample into digital microfluidic chip, and separating sample droplet from droplet with functional modification magnetic bead by electrode;After mixing two droplets, add vesicle binding solution and mix, then incubate;Through magnetic control fixed magnetic bead, remove solution, then add deionized water to clean magnetic bead;After removing magnetic bead cleaning solution, add vesicle eluent and incubate with magnetic bead;Separate out the droplet containing extracellular vesicle;Transfer the droplet containing extracellular vesicle to the upper of gold-coated glass, remove droplet after incubation;Use deionized water to clean hybridized gold-coated glass;Use self-interference spectrum imaging system to detect vesicle quantity and its particle size distribution.The application can realize extracellular vesicle quantitative counting, particle size measurement and surface protein marker detection simultaneously, and improve single vesicle quantitative imaging precision and detection efficiency.

The invention discloses a bio-based antibacterial agent based on yeast fermentation and a preparation method of the bio-based antibacterial agent in the field of antibacterial agents. The bio-based antibacterial agent comprises the following components in parts by weight: 2-4 parts of modified chitosan, 1 part of modified polyethylene glycol, 0.2-0.3 part of pig gastric mucoprotein and 0.6-0.8 part of sodium alginate. According to the invention, a gene corresponding to an amino acid sequence of tachyplesin is optimized; according to the invention, a pYES2 / CT vector is used as a target gene, an EcoR I / Xho I restriction enzyme cutting site is introduced to a 5 ' / 3' end, it is ensured that the target gene can be accurately inserted into the pYES2 / CT vector, Tachyplesin-1 is a natural antibacterial peptide, a killing effect can be realized by destroying cell membranes of harmful bacteria and interfering metabolism of the harmful bacteria, and Tachyplesin breaks through intestinal environment limitation and accurately acts on the harmful bacteria through coating and functional modification of a modified vector.

The application provides a kind of high-efficiency tumor-targeting bacterial outer membrane vesicle and its preparation method, it is related to biological medicine technical field.The target protein PDL1 binding peptide is successfully modified on the surface of bacterial outer membrane vesicle to prepare bacterial outer membrane vesicle PDL1-OMVs, its particle size range is between 20-250nm, with obvious tea tray like double membrane structure, meet the structural characteristics of bacterial outer membrane vesicle.The PDL1-OMVs provided by the application improves the weak targeting efficiency of PD-L1 binding peptide on the one hand, significantly improves the targeting ability of PD-L1 binding peptide, on the other hand, realizes the targeted functional modification of bacterial outer membrane vesicle, and PDL1-OMVs can realize specific targeting effect on tumor cells, which provides conditions for it as a targeted delivery tumor drug carrier.

The application belongs to the technical field of organic synthesis and protein modification, and particularly relates to a 3-acyl-4-pyrone derivative as a coupling reagent and application thereof in preparation of polypeptide and protein conjugates. The 3-acyl-4-pyrone derivative can rapidly and efficiently chemoselectively undergo O to N insertion substitution reaction with a lysine residue in a polypeptide or protein sequence under near physiological conditions, so as to realize coupling of the polypeptide and the protein. The 3-acyl-4-pyrone derivative has a simple synthesis method, simple and convenient modification operation for the protein, novel structure, and has not been reported to be applied to polypeptide and protein modification. The 3-acyl-4-pyrone derivative provides a new technology and a new way for various functional modification of polypeptides and proteins.

The application discloses a kind of peptide micropipe / In2O3 / Au composite gas-sensitive materials, its preparation method and application, belong to gas-sensitive material technical field.The material is self-assembled peptide micropipe (SPMFs) as three-dimensional support skeleton, by solution mixing and functional modification, make In2O3 nanoparticle uniform dispersion, Au nanoparticle surface load, form ternary composite system.SPMFs porous structure provides stable load platform for nanoparticle and accelerates gas diffusion, the LSPR effect of Au nanoparticle can enhance visible light absorption and surface reactivity, the heterojunction formed by In2O3 and SPMFs regulates electron transport.Under the synergistic effect of the three, material can detect ethanol at room temperature under visible light excitation, with high selectivity, high sensitivity and fast adsorption and desorption characteristics, detection lower limit is as low as 1 ppm, meet the demand of trace ethanol real-time monitoring.

The present application relates to the technical field of dye preparation data processing, and particularly relates to an application method of liquid disperse dye based on nano material modification, which significantly improves the performance and application efficiency of the liquid disperse dye through surface functional modification of the nano material and multi-parameter collaborative optimization of the genetic algorithm. The modified nano surface amino functional group forms directional chemical bonding with the dye molecule, enhances the adsorption strength of the dye on the fiber surface and the thermal migration stability under high temperature environment, generates a globally optimal process combination through multi-objective iterative optimization, breaks through the limitations of low parameter matching efficiency and poor adaptability of the traditional empirical method, and reduces the COD value of dyeing wastewater. The present application realizes precise regulation and control of dyeing process parameters, efficient use of resources and closed-loop control of clean production, and takes into account dyeing quality, energy consumption optimization and environmental protection requirements.

The invention discloses a nano biosensor based on functionalized graphdiyne, construction of the nano biosensor and application of the nano biosensor in bacterial extracorporeal vesicle detection, and belongs to the technical field of nano biosensors. The invention relates to a nano biosensor based on functionalized graphdiyne (GDY). The nano biosensor comprises GDY-metal oxide nano enzyme, graphdiyne is used as a substrate material, and metal oxide nanoparticles are loaded on the surface of the substrate material to form the heterogeneous composite material with a stable structure. According to the sensor, through rational design of a GDY-based nano sensing interface, efficient capture of bacterial extracellular vesicles is realized under the condition of not depending on ultracentrifugation, and eukaryotic extracellular vesicles and bacterial-derived extracellular vesicles are effectively distinguished. In order to reduce the detection cost, various metal nanoparticles are adopted for functional modification, and the capture process can be completed without using expensive biological recognition molecules.

The invention relates to the technical field of thrombus drug resistance detection, and discloses a thrombus diagnosis and treatment method for detecting thrombus drug resistance and performing accurate medication, which comprises the following steps: step (1), designing and constructing a nano diagnosis and treatment system; (2) thrombus drug resistance detection; and (3) carrying out precise thrombolysis medication treatment. According to the scheme, a nanometer material is used as a carrier, a nanometer diagnosis and treatment system is constructed through functional modification of targeted recognition, change response, thrombolytic drugs and the like, the thrombus drug resistance is diagnosed by detecting nucleic acid aptamer structure change caused by interaction of the nanometer diagnosis and treatment system and thrombus components, and accurate medication treatment is carried out according to the detection result.

The invention belongs to the technical field of environmental functional materials and environmental pollution treatment, and particularly relates to an alumina microsphere structure and surface characteristic regulation and control method and application thereof. According to the method, a hydrothermal synthesis method is taken as a core, and the sea urchin-shaped Al2O3 microspheres with three different structures, namely a solid structure, a core-shell structure and a hollow structure, are prepared by regulating and controlling the temperature and the reaction time of a hydrothermal reaction system. On the basis, directional regulation and control on the surface characteristics (specific surface area, surface charge and surface functional groups) of the Al2O3 product are further realized by changing the type of a solvent for washing the intermediate boehmite (AlOOH), and finally, the adsorption performance of the Al2O3 microspheres on tetracycline (TC) is remarkably improved. Therefore, the Al2O3 structure and the surface regulation strategy constructed by the invention not only can provide an innovative idea for functional modification of a traditional Al2O3 material, but also can provide a technical scheme with practical application value for efficient purification treatment of a tetracycline-polluted water body.

The invention discloses a method for detecting a tumor marker CA72-4 based on a sandwich-type electrochemical immunosensor, which comprises the following specific steps: constructing an rGO-TEPA / ZIF67-ZIF8 / Au functional modification layer as a CA72-4 capture antibody Ab1 fixing carrier by using a glassy carbon electrode as a substrate, and coupling AuPdRu ternary nanoparticles and a CA72-4 detection antibody Ab2 to form a signal probe; specific recognition of the tumor marker CA72-4 is realized through an Ab1 / CA72-4 / Ab2 sandwich structure, and high-sensitivity selective detection of a target antigen, namely the tumor marker CA72-4, is realized by utilizing an amplified current signal of bimetal / trimetal synergistic catalysis H2O2 reaction. By combining multi-metal catalysis and the high-conductivity composite substrate, bidirectional signal amplification is realized, and the electrochemical immunodetection sensitivity is remarkably improved. The method is simple and convenient to operate, high in sensitivity and rapid in response, and can be popularized and applied to detection of other tumor markers.

The application discloses a kind of special membrane material for targeting trapping Corbicula fluminea larvae in water and a preparation method thereof, and belongs to the field of water environmental biological pollution prevention and control.The membrane material is a multilayer composite structure, including a nanofiber membrane substrate layer, an interface adhesion layer and a functional modification layer.The interface adhesion layer is a biomimetic condensed layer gel formed by hydrophobic polyelectrolyte and phytic acid solidification;The functional modification layer is covalently grafted with a biomimetic adhesion protein active peptide segment and a Corbicula fluminea larva chemical information substance.The material actively lures larvae by chemical information substance, and realizes high-strength adhesion capture by using a biomimetic peptide segment rich in aromatic and cationic amino acids, and is integrated with "trapping body".The preparation method includes the steps of electrospinning substrate, surface activation, coating gel and functional modification, etc.The material has high selectivity and high capture rate for Corbicula fluminea larvae, and is environmentally friendly, long-acting and stable, and is suitable for green prevention and control of Corbicula fluminea larvae pollution in facilities such as water conveying pipeline and pump station.