25 results about "Functional modification" patented technology

The invention provides a preparation method of a substrate of an external vesicle functional marker rapid detection kit, and belongs to the technical field of external vesicle functional marker detection.The preparation method comprises the steps that a high-purity coating working solution is prepared, and precise spraying is achieved through a piezoelectric type ink jet system; substrate functional modification is completed through confining liquid treatment and labeled antibody working solution preparation, a double-layer game model containing detection sensitivity optimization and process stability optimization is established, and an optimal parameter combination is obtained through game iterative calculation by combining the synergistic effect of a quality evaluation function and a stability control function. A characteristic parameter database is established to store historical production data and quality detection results, a multi-parameter joint evaluation method is adopted to calculate a comprehensive score and carry out unified product grading, and a quality control system and a standardized parameter optimization mechanism are driven through full-process data. The technical problem that the quality consistency difference is large in the batch production process is solved.

The invention discloses an up-conversion nano-composite with a core-shell structure, a preparation method and application, and belongs to the technical field of biomedical bimodal nano-probes. According to the nanocomposite, up-conversion nanoparticles (UCNPs) serve as a core, metal-organic frameworks (MOFs) serve as a shell layer, horse radish peroxidase (HRP) is loaded on the shell layer, and UCNP (at) MOF / HRP is formed. Subsequent biological functional modification, such as coupling of an antibody, an antigen, nucleic acid and other substances, can be carried by an activating group or a metal coordination bond carried by a shell ligand of the upconversion nanocomposite MOF with the core-shell structure, so that the functions of specific recognition, targeted capture, specific loading and the like are realized.

The invention provides an iron-based nano material, a preparation method and application, the material comprises magnetic iron oxide nano particles and a functional modification layer on the surface, the magnetic iron oxide nano particles are Fe3O4, the particle size is 10-30 nm, and the magnetic iron oxide nano particles have specific oxygen vacancy concentration, 337 nm laser absorptivity and saturation magnetization; the functional modification layer is an amino-containing polymer layer, such as a polydopamine or cysteine-polyethyleneimine copolymer, and can reduce salt ion interference. The material solves the problems of low molecular weight region background interference, non-uniform crystallization, low ionization efficiency, poor salt tolerance and the like during detection of 100-400Da small molecular metabolites in trace samples such as tears and the like by optimizing magnetic responsiveness, photo-thermal conversion capability and salt resistance of an existing matrix; the method realizes high sensitivity and high repeatability (detection), and is suitable for metabonomics analysis of trace biological samples.

The invention discloses a special membrane material for targeted trapping of macrobrachium larvae in water and a preparation method of the special membrane material, and belongs to the field of water environment biological pollution prevention and control. The membrane material is of a multi-layer composite structure and comprises a nanofiber membrane base material layer, an interface adhesion layer and a functional modification layer. The interface adhesion layer is bionic coacervate gel formed by curing hydrophobic polyelectrolyte and phytic acid; the functional modification layer is covalently grafted with a bionic adhesion protein active peptide fragment and a macrobrachium larva chemical pheromone. The material actively attracts larvae to approach through chemical pheromones, high-strength adhesion capture is achieved through bionic peptide fragments rich in aromatic and cationic amino acids, and trapping is integrated. The preparation method comprises the steps of electrostatic spinning of a base material, surface activation, gel coating, functional modification and the like. The material disclosed by the invention has high selectivity and high capture rate on the larvae of the Limnoperna fortunei, is environment-friendly, long-acting and stable, and is suitable for green prevention and treatment of fouling of the larvae of the Limnoperna fortunei in facilities such as water pipelines and pump stations.

The present application belongs to the technical field of extracellular vesicle separation and analysis, and particularly relates to a portable extracellular vesicle separation and analysis system with bubble micro-group self-driven scanning, which comprises a polytetrafluoroethylene micro-flow tube, a dyeing agent, a bubble robot capture carrier with surface antibody functional modification, and a detection module. During operation, the bubble robot is driven by buoyancy to perform reciprocating scanning movement along the axial direction of the tube by tilting the micro-flow tube, so as to actively and efficiently capture target extracellular vesicles in the sample. After capture, the bubble robot is detected by using a fluorescently labeled ssDNA dyeing agent, and the bubble robot transferred to the observation carrier is subjected to fluorescent signal analysis by the detection module. The system integrates new materials and micro-flow control technology, realizes rapid and portable separation and quantitative analysis of extracellular vesicles, solves the problems of existing extracellular vesicle separation technology, such as complicated operation, dependence on large equipment and difficulty in realizing rapid on-site detection, and is particularly suitable for on-site detection of clinical samples.

The application relates to a kind of lipid nanocarriers coupled with GO-203 and loaded with DMAMCL and its preparation method and application, belong to the field of biological medicine and nano-preparation.The lipid nanocarriers include water phase core, lipid bilayer and surface modification layer from inside to outside, wherein water-soluble dimethylamine methyl chloride (DMAMCL) is loaded in the water phase core;The lipid bilayer is wrapped in the water phase core, and coumarin-6 is embedded as fluorescent marker for tracing and detection;Functional modification layer is arranged on the outer surface of the lipid bilayer, including DSPE-PEG2000-COOH and DSPE-PEG2000-GO-203, wherein GO-203 is cell membrane penetrating peptide.The lipid nanocarrier provided by the application can realize the synergistic delivery of GO-203 and DMAMCL, be used for regulating tumor immune microenvironment and be used for the treatment of triple-negative breast cancer.

The invention belongs to the field of food science and microbial engineering, and particularly relates to fusarium venanii protein, low-lipoprotein food as well as a preparation method and application of the fusarium venanii protein and low-lipoprotein food. The invention provides a fusarium venanii TB01 protein with high emulsibility as well as a preparation method and application of the fusarium venanii TB01 protein. According to the method, fusarium venanii TB01 mycelium is adopted as a raw material, composite enzymolysis and ultrasonic treatment are innovatively and sequentially cooperated, and functional modification of a protein structure is achieved while high-content protein (larger than or equal to 82%) is extracted through efficient wall breaking. Tests show that the two key indexes of emulsifying activity and stability of the obtained protein are obviously superior to those of soybean protein, and the emulsifying stability of the obtained protein is superior to that of commercial whey protein. Therefore, the protein obtained by the invention can be used as a high-performance emulsifying ingredient, is used for replacing or partially replacing whey protein or soybean protein, and is applied to food systems such as beverages, plant yoghurt, sauce and the like.

The application provides an electroluminescence-enhanced biosensor and a preparation method thereof. The electroluminescence-enhanced biosensor comprises, from bottom to top, a flexible substrate, a coplanar electrode layer, a mixed luminescence layer, a functional modification layer and a biological recognition layer which are sequentially stacked; the coplanar electrode layer is formed by forming a pair of parallel electrodes on the flexible substrate with a conductive material; the mixed luminescence layer is formed by uniformly coating an alternating current electroluminescence material on the coplanar electrode layer; the functional modification layer comprises an adhesion layer arranged on the mixed luminescence layer and a material layer arranged on the adhesion layer; the adhesion layer is made of a positively charged biocompatible polymer, and the material layer is made of a two-dimensional material containing a phosphate group; the biological recognition layer is formed by fixing specific antibodies on the material layer and then sealing unreacted sites with a sealing material; after a sample to be measured is added to the biological recognition layer, an alternating current voltage is applied, and a detection signal is read.

The present application belongs to the field of food science and microbial engineering, and specifically relates to a fusarium venenatum protein, a low-fat protein food and a preparation method and use thereof. The present application provides a high-emulsifying fusarium venenatum TB01 protein, a preparation method and application thereof. The present application uses fusarium venenatum TB01 mycelium as raw material, and innovatively realizes the functional modification of protein structure by sequentially cooperating complex enzymolysis and ultrasonic treatment, thereby efficiently breaking the cell wall to extract high-content protein (≥82%) and realizing the functional modification of protein structure. Tests show that the emulsification activity and stability of the obtained protein are significantly better than those of soybean protein, and the emulsification stability is better than that of commercial whey protein. Therefore, the protein obtained by the present application can be used as a high-performance emulsifying ingredient to replace or partially replace whey protein or soybean protein, and is applied to beverage, plant yogurt, sauce and other food systems.

The invention discloses a fluorescence analysis and detection method and system based on quantum dots, and belongs to the technical field of analysis and detection. According to the method, functional modified quantum dots are used as fluorescent probes, a fluorescence detection system is constructed through specific recognition reaction, and high-sensitivity and high-specificity detection of a target detection object is realized by combining collection and quantitative analysis of fluorescence signals. The corresponding system comprises an excitation light source module, a sample reaction module, a fluorescence detection module, a data processing module and a control module, all the modules work cooperatively, key parameters in the detection process can be precisely regulated and controlled, and the detection precision and stability are improved. The method solves the problems of poor probe stability, low quantum yield and high detection limit in a traditional fluorescence detection method, has the advantages of high detection speed, high specificity and wide application range, and can be widely applied to the fields of food safety detection, environmental pollutant monitoring, biomedical diagnosis and the like.

The invention discloses a nano-microsphere time-resolved fluorescent probe as well as a preparation method and application thereof. The preparation method comprises the following three steps: preparation of core-layer nano microspheres, shell-layer functional modification and fluorescence enhancement and stabilization treatment. A polylactic acid-glycolic acid copolymer (PLGA) is used as a core-layer carrier to load a rare earth ion complex, and a SiO2 coating layer and a targeting ligand are combined for modification, so that the nano-microsphere fluorescent probe with a core-shell structure is prepared. The probe has the characteristics of long fluorescence lifetime (5-8ms), high quantum yield (greater than or equal to 35%), strong stability, excellent targeting property and the like, and can be widely applied to food safety detection (such as quinolone antibiotics and microcystin MC-LR) and environmental pollution detection (such as heavy metal ions such as Hg < 2 + > and Pb < 2 + >), and the detection limit is as low as 0.05 ng / mL or 0.1 nmol / L. The problems that a traditional fluorescent probe is low in loading capacity, poor in stability and insufficient in targeting property are solved, and the method has remarkable industrial application value.

The application provides a kind of extracellular vesicle quantitative detection method based on self-interference spectrum reconstruction, it is related to the intersection field of biomedical detection and optical engineering.The method comprises: adding clinical sample into digital microfluidic chip, and separating sample droplet from droplet with functional modification magnetic bead by electrode;After mixing two droplets, add vesicle binding solution and mix, then incubate;Through magnetic control fixed magnetic bead, remove solution, then add deionized water to clean magnetic bead;After removing magnetic bead cleaning solution, add vesicle eluent and incubate with magnetic bead;Separate out the droplet containing extracellular vesicle;Transfer the droplet containing extracellular vesicle to the upper of gold-coated glass, remove droplet after incubation;Use deionized water to clean hybridized gold-coated glass;Use self-interference spectrum imaging system to detect vesicle quantity and its particle size distribution.The application can realize extracellular vesicle quantitative counting, particle size measurement and surface protein marker detection simultaneously, and improve single vesicle quantitative imaging precision and detection efficiency.

The invention discloses a bio-based antibacterial agent based on yeast fermentation and a preparation method of the bio-based antibacterial agent in the field of antibacterial agents. The bio-based antibacterial agent comprises the following components in parts by weight: 2-4 parts of modified chitosan, 1 part of modified polyethylene glycol, 0.2-0.3 part of pig gastric mucoprotein and 0.6-0.8 part of sodium alginate. According to the invention, a gene corresponding to an amino acid sequence of tachyplesin is optimized; according to the invention, a pYES2 / CT vector is used as a target gene, an EcoR I / Xho I restriction enzyme cutting site is introduced to a 5 ' / 3' end, it is ensured that the target gene can be accurately inserted into the pYES2 / CT vector, Tachyplesin-1 is a natural antibacterial peptide, a killing effect can be realized by destroying cell membranes of harmful bacteria and interfering metabolism of the harmful bacteria, and Tachyplesin breaks through intestinal environment limitation and accurately acts on the harmful bacteria through coating and functional modification of a modified vector.

The application provides a kind of high-efficiency tumor-targeting bacterial outer membrane vesicle and its preparation method, it is related to biological medicine technical field.The target protein PDL1 binding peptide is successfully modified on the surface of bacterial outer membrane vesicle to prepare bacterial outer membrane vesicle PDL1-OMVs, its particle size range is between 20-250nm, with obvious tea tray like double membrane structure, meet the structural characteristics of bacterial outer membrane vesicle.The PDL1-OMVs provided by the application improves the weak targeting efficiency of PD-L1 binding peptide on the one hand, significantly improves the targeting ability of PD-L1 binding peptide, on the other hand, realizes the targeted functional modification of bacterial outer membrane vesicle, and PDL1-OMVs can realize specific targeting effect on tumor cells, which provides conditions for it as a targeted delivery tumor drug carrier.

The application discloses a kind of peptide micropipe / In2O3 / Au composite gas-sensitive materials, its preparation method and application, belong to gas-sensitive material technical field.The material is self-assembled peptide micropipe (SPMFs) as three-dimensional support skeleton, by solution mixing and functional modification, make In2O3 nanoparticle uniform dispersion, Au nanoparticle surface load, form ternary composite system.SPMFs porous structure provides stable load platform for nanoparticle and accelerates gas diffusion, the LSPR effect of Au nanoparticle can enhance visible light absorption and surface reactivity, the heterojunction formed by In2O3 and SPMFs regulates electron transport.Under the synergistic effect of the three, material can detect ethanol at room temperature under visible light excitation, with high selectivity, high sensitivity and fast adsorption and desorption characteristics, detection lower limit is as low as 1 ppm, meet the demand of trace ethanol real-time monitoring.

The present application relates to the technical field of dye preparation data processing, and particularly relates to an application method of liquid disperse dye based on nano material modification, which significantly improves the performance and application efficiency of the liquid disperse dye through surface functional modification of the nano material and multi-parameter collaborative optimization of the genetic algorithm. The modified nano surface amino functional group forms directional chemical bonding with the dye molecule, enhances the adsorption strength of the dye on the fiber surface and the thermal migration stability under high temperature environment, generates a globally optimal process combination through multi-objective iterative optimization, breaks through the limitations of low parameter matching efficiency and poor adaptability of the traditional empirical method, and reduces the COD value of dyeing wastewater. The present application realizes precise regulation and control of dyeing process parameters, efficient use of resources and closed-loop control of clean production, and takes into account dyeing quality, energy consumption optimization and environmental protection requirements.

The invention discloses a nano biosensor based on functionalized graphdiyne, construction of the nano biosensor and application of the nano biosensor in bacterial extracorporeal vesicle detection, and belongs to the technical field of nano biosensors. The invention relates to a nano biosensor based on functionalized graphdiyne (GDY). The nano biosensor comprises GDY-metal oxide nano enzyme, graphdiyne is used as a substrate material, and metal oxide nanoparticles are loaded on the surface of the substrate material to form the heterogeneous composite material with a stable structure. According to the sensor, through rational design of a GDY-based nano sensing interface, efficient capture of bacterial extracellular vesicles is realized under the condition of not depending on ultracentrifugation, and eukaryotic extracellular vesicles and bacterial-derived extracellular vesicles are effectively distinguished. In order to reduce the detection cost, various metal nanoparticles are adopted for functional modification, and the capture process can be completed without using expensive biological recognition molecules.

The invention discloses a method for detecting a tumor marker CA72-4 based on a sandwich-type electrochemical immunosensor, which comprises the following specific steps: constructing an rGO-TEPA / ZIF67-ZIF8 / Au functional modification layer as a CA72-4 capture antibody Ab1 fixing carrier by using a glassy carbon electrode as a substrate, and coupling AuPdRu ternary nanoparticles and a CA72-4 detection antibody Ab2 to form a signal probe; specific recognition of the tumor marker CA72-4 is realized through an Ab1 / CA72-4 / Ab2 sandwich structure, and high-sensitivity selective detection of a target antigen, namely the tumor marker CA72-4, is realized by utilizing an amplified current signal of bimetal / trimetal synergistic catalysis H2O2 reaction. By combining multi-metal catalysis and the high-conductivity composite substrate, bidirectional signal amplification is realized, and the electrochemical immunodetection sensitivity is remarkably improved. The method is simple and convenient to operate, high in sensitivity and rapid in response, and can be popularized and applied to detection of other tumor markers.

The application discloses a kind of special membrane material for targeting trapping Corbicula fluminea larvae in water and a preparation method thereof, and belongs to the field of water environmental biological pollution prevention and control.The membrane material is a multilayer composite structure, including a nanofiber membrane substrate layer, an interface adhesion layer and a functional modification layer.The interface adhesion layer is a biomimetic condensed layer gel formed by hydrophobic polyelectrolyte and phytic acid solidification;The functional modification layer is covalently grafted with a biomimetic adhesion protein active peptide segment and a Corbicula fluminea larva chemical information substance.The material actively lures larvae by chemical information substance, and realizes high-strength adhesion capture by using a biomimetic peptide segment rich in aromatic and cationic amino acids, and is integrated with "trapping body".The preparation method includes the steps of electrospinning substrate, surface activation, coating gel and functional modification, etc.The material has high selectivity and high capture rate for Corbicula fluminea larvae, and is environmentally friendly, long-acting and stable, and is suitable for green prevention and control of Corbicula fluminea larvae pollution in facilities such as water conveying pipeline and pump station.

The invention relates to a two-dimensional metal polyphenol framework material as well as a preparation method and application thereof. The framework material is a two-dimensional sheet structure material formed by coordination of metal ions and flavonoid polyphenol molecules. Wherein the molar ratio of the metal ions to the flavonoid polyphenol is (5-20): 1; the particle size of the two-dimensional metal polyphenol framework material is 200 to 800 nm; and the thickness of the two-dimensional metal polyphenol framework material is 2 to 10 nm. Compared with the prior art, the two-dimensional metal polyphenol framework material prepared by the invention is simple, convenient and green in synthesis process, and does not need high temperature, high pressure and toxic organic solvents; the material has excellent biocompatibility and physiological stability and is suitable for biomedical application; the specific surface area is high, and drug loading and functional modification are facilitated; the structure, the size and the composition are adjustable, the flavonoid polyphenol endows the compound with various biological activities such as oxidation resistance and inflammation resistance, and the compound has wide potential in the fields of drug delivery, biological imaging, catalysis, energy environment and the like.

The invention discloses a preparation method of a hematoxylin imprinted nano material based on an ultralow-density magnetic carrier. The preparation method comprises the following steps: firstly, preparing a nano magnetic hollow carrier by utilizing a pore-foaming agent and a solvothermal method, and then carrying out boric acid functional modification on the surface of the carrier through chemical modification. And finally, adding the nano boric acid nano magnetic hollow carrier and hematoxylin into a solvent for prepolymerization, then adding a cross-linking agent and a catalyst, and preparing the hematoxylin imprinted material by using a surface molecular imprinting technology. The hematoxylin imprinted material prepared by the invention has an ultralow-density magnetic carrier, shows excellent magnetic response enrichment capacity, has excellent specific recognition capacity and efficient adsorption capacity on hematoxylin, and can be used for rapid, efficient and selective adsorption and detection of hematoxylin in a complex environment.

The application discloses a biological preparation method of a zein film and belongs to the technical field of natural high polymer material functional modification. In the application, a reducing agent L-cysteine is used to reduce disulfide bonds in zein, so that the molecular structure is loose, and more glutamine residues are exposed. On the basis, a transglutaminase catalytic acyl transfer reaction is used to introduce epsilon-polylysine rich in primary amino groups into the protein structure, so that the protein film with high physical and mechanical properties, water-soluble stability and high antibacterial activity is prepared, and the application effect of the protein film as food preservation material is improved.

The invention discloses a method for promoting CAR (chimeric antigen receptor) aggregation by forming a kinetic trap effect through DNA (deoxyribonucleic acid) nanogel, and relates to the technical field of crossing of immunotherapy and biomechanics, and the method comprises the following steps: (1) preparing DNA nanogel NG which is formed by self-assembling two Y-type DNA monomers, namely YMA and YMB through complementary cohesive end sequences, and bridging through a DNA linker LK to form a cross-linked network; and (2) carrying out modification and anti-nuclease protection treatment on the NG, namely carrying out functional modification on the NG by adopting NHS ester so that the NG is covalently bound with free sulfydryl on the surface of the CAR-T cell, and wrapping the NG by using a comb-type cationic copolymer CCC so as to enhance the stability. According to the method for promoting CAR aggregation by forming the dynamic trap effect through the DNA nanogel, the dynamic trap effect of the DNA nanogel (NG) is utilized to regulate and control CAR spatial distribution, CAR receptors are forced to aggregate through physical steric hindrance, and the limitation of a traditional chemical modification method is overcome.

The application discloses a particle sorting chip and method based on chromatography and DLD cooperative work, and belongs to the field of micro-nano fluidic chips and biological particle sorting. The chip comprises a chip substrate, an inlet convergence area, a separation channel and an outlet separation area; the separation channel is provided with a periodically staggered micro-pillar array, and the surface of the micro-pillar array is provided with a functional modification layer. The application combines the size sorting principle of DLD with the chromatography separation principle based on surface interaction, breaks the flow field symmetry through the periodically staggered micro-pillar array (offset of adjacent columns) of DLD, realizes size-dependent particle separation, and makes the target particles and the columns produce selective interaction through the functional modification layer on the surface of the columns, so as to regulate the transverse deflection angle / migration speed of the target particles. The application breaks through the limitation of traditional DLD technology which only depends on size difference through the dual sorting dimensions of size screening and surface interaction, can efficiently separate biological particles with similar sizes but different physical and chemical properties, and significantly improves the separation purity and resolution.

The present application relates to the technical field of bio-based formaldehyde-free adhesive, and aims to provide a preparation method of high-strength and high-toughness bio-based adhesive and application thereof. The present application constructs a boron nitride nanosheet based on thiol click chemistry functional modification through interface molecular structure design, innovates interface mechanical interlocking and chemical multi-network crosslinking reinforcement and toughening integrated technology, provides a preparation method of boron nitride nanosheet intercalation modification of plant protein adhesive, significantly improves the poor interfacial adhesion of the adhesive and the wood surface, and obtains a bio-based adhesive with good water resistance, high strength, excellent toughness and wider application range. The high-performance, high-strength and high-toughness bio-based adhesive prepared by the present application has a dry shear strength of 2.56 MPa, a wet shear strength of 1.32 MPa and a wet debonding work of 1800 mJ when hot-pressed into plywood, showing excellent strength and toughness, and the thermal stability and flame retardance of the adhesive are significantly enhanced, which has a broad industrial application prospect.