This invention belongs to the technical field of
plant extract nanoemulsion preparation. To address the current limited research on flavonoids in
jujube seed, particularly regarding the extraction, separation, and purification of total flavonoids from
jujube seed, this invention provides a
jujube seed total
flavonoid nanoemulsion, its preparation method, and applications. The process involves extracting fatty oil from jujube seed, enzymatically hydrolyzing jujube seed
powder, fermenting the jujube seed enzymatic
hydrolysate, extracting total flavonoids, purifying the total flavonoids, and preparing the nanoemulsion using a self-emulsification method. The resulting jujube seed total
flavonoid nanoemulsion exhibits high purity, a simple process, and is
environmentally friendly and safe. It shows promising applications in the preparation of drugs to improve
insomnia, depression, and
anxiety. It provides excellent encapsulation and delivery capabilities, protecting the total flavonoids from hydrolytic enzymes, gastrointestinal pH, and other environmental conditions, promoting
drug absorption, and improving
drug bioavailability and brain penetration. It easily crosses intestinal intercellular spaces and the
epithelial barrier, increasing the cellular
uptake rate of jujube seed total flavonoids and enhancing the
brain targeting and effectiveness of
in vivo drug administration.