The invention discloses a biological tissue photoacoustic circulating tumor cell detection and diagnosis device. The device is provided with a high-frequency pulse laser, a microscope and a ultrasonic probe, and a detected object is arranged between the microscope and the ultrasonic probe so that laser generated by the high-frequency pulse laser can be focused on the detected object through the microscope. The invention further discloses a detection and diagnosis method of the detection and diagnosis device. Due to the adoption of the technical scheme, the defect that fluorescent markers interfere in the biological tissue physiological environment and have cytotoxicity on normal blood cells and lymphocytes is overcome. Non-invasive detection is achieved, haemospasia is avoided, and marking is not needed; data difference between animal individuals is avoided, and the number of animals needed during an animal experiment is greatly reduced; the application range is wide, and the biological tissue photoacoustic circulating tumor cell detection and diagnosis device can be widely applied to the immunology, hematology, oncology, cytobiology, cytogenetics, biochemistry, clinical medicine and basic medicine research fields; the device is simple in structure, convenient to operate and high in safety.