7 results about "Cytogenetics" patented technology

Genome whole chromosome analysis is a critical gold standard method for clinicians to study molecular lesions at single cell level in diseases and cancers. However, they suffer from low-sample-yielding, labor-intensive procedures that require subjective manual involvement of highly trained professionals. Here we disclose new devices, systems, and methods to automate, standardize, and accelerate the digital cytopathological sample and data generation with improved data quality to enable precision medicine and improve clinical practice. This disclosure describes a family of micro-fluidic devices, and accompanying methods for using said devices, to perform cytogenomic analysis on cells, nucleus, vesicles exosomes and their respective chromosomes, chromatins, nucleic acid polymers, subcellular genomic elements and any other dynamic transitional states between these forms. Collectively, these microfluidic devices allow for the consistent physical manipulation and preparation of macromolecules to substantially improve the quality of the of the cytogenetic information being interrogated.

The invention provides a treatment response model for acute myelogenous leukemia (AML) accompanied with myelodysplastic syndrome related genetic abnormality (MRGA) and application thereof, and the model combines clinical data, cytogenetics and molecular biology data of patients. Variables significantly related to the complete remission rate of the patient are screened out through Lasso regression and Cox multivariable regression analysis, a treatment response model for predicting the complete remission rate is constructed based on the variables, and a combined scoring system formed by the treatment response model and a constructed survival risk scoring model is used for predicting the response of the patient to induced treatment and 1-3-year survival prognosis. And an individualized basis is provided for clinical treatment decision-making of the AML patient.

The invention discloses a biomarker combination for predicting survival prognosis of an acute myeloid leukemia patient and application of the biomarker combination, and belongs to the technical field of molecular biomedicine. It is found for the first time that the specific N-sugar chain level in a blood sample during definite diagnosis of an AML patient is significantly related to survival prognosis. Through the screened N-carbohydrate chain marker, a Cox regression analysis is utilized to construct an AML prognosis prediction scoring system (AML-GPSS). According to the system, prognosis scores are obtained through a quantitative calculation formula, and patients are divided into different prognosis risk groups according to a preset critical value. The invention provides a brand-new prognosis prediction model based on a protein post-translational modification function, a detection sample is easy to obtain, and the method is stable; an important bioinformatics tool and a new clinical practice scheme are provided for making up for the defects of an existing cytogenetics layering system and achieving early-stage accurate risk layering and individualized treatment decision making of AML patients.

The invention discloses a method for preparing tobacco metaphase chromosomes by utilizing young tobacco plants and application, and belongs to the technical field of preparation of tobacco chromosome specimens. The method comprises the following steps: germinating tobacco seeds at room temperature for 5-7 days, stripping seed shells when the seeds germinate and radicles grow to about 2 mm, sequentially performing N2O treatment on seedlings with radicles, fixing with a stationary liquid, performing enzymolysis in an enzymatic hydrolysate, lightly grinding plant tissues by using a sterile grinding rod until cells are dispersed, centrifuging, airing, adding glacial acetic acid, performing uniform vortex mixing, and performing freeze drying to obtain the tobacco seed suspension. And dripping a small amount of suspension on a clean glass slide to prepare the tobacco metaphase chromosome specimen. According to the method, materials are easy to obtain, the period is extremely short, operation is safe, the chromosome quality is high, and a reliable and efficient standardized scheme is provided for tobacco cytogenetics, molecular cytology and breeding research.

The embodiment of the present application discloses a kind of intelligent chromosome harvesting systems, belong to the technical field of cytogenetics detection.It includes: oscillation module, for executing oscillation operation to test tube;Centrifugal module, for executing centrifugation operation;Liquid extraction module, for extracting supernatant;Image acquisition module, for collecting sample image after oscillation and / or after centrifugation;Intelligent analysis module, for identifying test tube boundary frame, and analyzing image in frame to identify the mixing state and / or centrifugation state of sample;Control module, for: when sample is not mixed, drive oscillation module to repeat oscillation until mixing;When it has been mixed, drive centrifugal module to centrifuge;When sample is not layered, drive centrifugal module to repeat centrifugation or alarm;When it has been layered, drive liquid extraction module to extract supernatant.The present application can realize closed loop oscillation mixing, automatically judge layering and full-process automatic control.

The invention belongs to the technical field of cell culture, and discloses a bone marrow cell culture medium and a preparation method thereof, and a method for in-vitro culture of bone marrow cells, the bone marrow cell culture medium is composed of a basic culture medium, streptomycin, gentamicin, a platelet lysis buffer, and glutamate enzyme; the final concentration of streptomycin is 50-100 [mu] g / mL, the final concentration of gentamicin is 20-50 [mu] g / mL, the final concentration of platelet lysate is 5-15% (v / v), and the final concentration of glutamate enzyme is 0.1-1 mmol / L. The bone marrow cell culture medium can efficiently support in-vitro proliferation of bone marrow cells, through specific combination and appropriate concentration control of the culture medium, the medium-term cell acquisition amount can be remarkably increased, and medium-term cell chromosomes are complete in morphology and good in dispersity; the standard requirements of clinical bone marrow cell chromosome karyotype analysis, blood disease diagnosis and cell genetics experiments in the scientific research field can be met.

The present application relates to the technical field of plant chromosome preparation, and specifically discloses a method for increasing the number of mitotic metaphase chromosomes of sugarcane or sugarcane relative genus root tips, comprising: soaking sugarcane or sugarcane relative genus stem double bud segments in an indole-3-acetic acid solution with a concentration of 60-120 mg / L, and culturing in sand or peat substrate; root tip pretreatment: culturing for 2-3 days, selecting healthy and tender root tips, and placing the root tips in saturated p-dichlorobenzene aqueous solution for low-temperature treatment at 8-12 DEG C for 3-5 h; using Carnoy's fixative for fixation; enzymolysis of the root tip growth point; smearing the root tip on a glass slide for preparation; and staining treatment. The method for preparing the present application can achieve the fastest time of only 4 days from root tip culture to chromosome observation, has a short cycle, high efficiency, is convenient and fast for sampling, has a large number of metaphase division phases in the chromosomes, clear morphology, a clean glass slide background, and is convenient for chromosome counting observation and karyotype analysis, and has important significance for the development of sugarcane cytogenetics research.