15 results about "Cytogenetics" patented technology

The present invention belongs to the field of biomedicine, and specifically relates to a prognostic model for predicting the overall survival rate of AML patients based on 9 fatty acid metabolism-related genes. The present invention analyzed the gene expression profiles of 354 AML patients from TCGA and VIZOME, determined the prognosis-related genes based on the fatty acid metabolism-related genes of the TCGA dataset using univariate Cox regression analysis and survival analysis, and established a prognostic model with 9 fatty acid-related genes. This model can evaluate the prognosis of AML patients more specifically and sensitively. Then, using a nomogram, the fatty acid metabolism gene prognostic model, age, and cytogenetic risk degree were integrated into a scoring system to more accurately predict the survival of AML patients, with broad clinical application prospects.

The invention relates to the field of biomedical detection, and provides a novel chromosome analysis smear processing system and method. The system is based on a full-automatic sample treatment platform and comprises an automatic cell inoculation and culture module, an automatic cell harvesting module, an automatic cell dropping module and a cell genetic information management system, the single-batch treatment flux is not less than 192, and the system can be expanded. And precise control of each link is realized through technologies of a high-precision split charging pump, electric field assisted cell separation and the like. The method comprises the steps of automatic inoculation, constant-temperature culture, electric field assisted harvesting, precise dropping and data monitoring and analysis. Compared with a traditional mode, the method solves the problems of complicated equipment, low flux, insufficient standardization and the like, has the advantages of high processing flux, high standardization and informatization degree, small operation error, intelligent quality control and the like, and can effectively improve the cytogenetics analysis efficiency and accuracy.

The invention discloses a peripheral blood lymphocyte culture standardization process, and relates to the technical field of cell culture, and the peripheral blood lymphocyte culture standardization process comprises the following steps: S1, blood sample planting; s2, cell culture; s3, harvesting the cells; s4, wafer dropping; s5, carrying out banding treatment; and S6, scanning and analyzing. The standardized process intends to standardize the whole peripheral blood chromosome preparation process, manual errors are reduced through instrument operation, experience judgment is replaced with specific numerical values, the standard operation process can avoid the slide preparation gap caused by human factors, then the peripheral blood chromosome karyotype analysis resolution is improved, a guarantee is provided for the reliability of clinical results, and the method is suitable for large-scale popularization and application. The step is the key of cytogenetics research, and detailed cell and chromosome data are provided; the standardized process aims to ensure that high-quality data output can be achieved in the culture, treatment and analysis process of the lymphocytes, so that the final analysis result is accurate and reliable.

Genome whole chromosome analysis is a critical gold standard method for clinicians to study molecular lesions at single cell level in diseases and cancers. However, they suffer from low-sample-yielding, labor-intensive procedures that require subjective manual involvement of highly trained professionals. Here we disclose new devices, systems, and methods to automate, standardize, and accelerate the digital cytopathological sample and data generation with improved data quality to enable precision medicine and improve clinical practice. This disclosure describes a family of micro-fluidic devices, and accompanying methods for using said devices, to perform cytogenomic analysis on cells, nucleus, vesicles exosomes and their respective chromosomes, chromatins, nucleic acid polymers, subcellular genomic elements and any other dynamic transitional states between these forms. Collectively, these microfluidic devices allow for the consistent physical manipulation and preparation of macromolecules to substantially improve the quality of the of the cytogenetic information being interrogated.

The invention discloses a flaking method for chromosomes of diptera insects, and relates to the technical field of cytogenetics. The preparation method of the fruit fly chromosome comprises the following steps: by taking a fruit fly insect as an example, taking a male fruit fly subjected to primary eclosion, anesthetizing, performing microinjection of colchicine on the abdomen, and putting the fruit fly with recovered vitality into a centrifugal tube; dissecting the fruit flies which are injected with colchicine for 2-4 hours, taking out testis, putting the testis on a glass slide, and dripping normal saline into the testis; a 0.5-1.5 mol / L HCl solution is dropwise added to the testis treated with the normal saline for dissociation, then a 0.05-0.07 mol / L KCl solution is dropwise added for hypotonic treatment, and then improved phenol fuchsin dye liquor is dropwise added for dyeing; and finally, carrying out color separation, tabletting and microscopic examination. According to the method, the testis are selected as observation materials, colchicine is injected into the abdomen for 2-4 h, then the testis are taken out for flaking, the problems that chromosomes are not clear, observation and research are not facilitated and the like when chromosome flaking is conducted on diptera insects with salivary glands as materials are solved, and finally the chromosomes with clear forms are obtained.

The invention provides a preparation method of lycoris plant root tip chromosomes, and belongs to the technical field of biology. The preparation method of the lycoris plant root tip chromosome comprises the steps of root tip induction culture, root tip collection and pretreatment, enzymolysis treatment, chromosome flaking, microscopic observation and the like. A normal-temperature and low-temperature combined 8-hydroxyquinoline double-stage pretreatment mode is adopted for newborn root tips, and a compound enzymatic hydrolysate and a buffer solution pre-preservation system with an optimized ratio are combined, so that the metaphase division cell proportion and the chromosome image quality are effectively improved. The method has the advantages of simplicity and convenience in operation, high efficiency, strong repeatability and the like, is suitable for preparing chromosomes of different types of lycoris radiate and hybrid materials thereof, and can also be popularized and applied to cytogenetics research of other bulb ornamental plants.

The invention relates to the technical field of plant breeding, in particular to a wheat powdery mildew resistance breeding method based on a double addition substitution line 21W155 and application of the wheat powdery mildew resistance breeding method based on the double addition substitution line 21W155. A progeny group is obtained by adding 2M chromosome immune to powdery mildew resistance and hybridizing and backcrossing the double addition substitution line 21W155 with 6B chromosome substituted by 6U and a susceptible variety Shanxi 225; a fluorescent in-situ hybridization technology of cytogenetics is combined with a powdery mildew resistance identification method, and 24W1666 of a 6U (6B) substitution line and 24W2103 of a 6U additional line which are immune to powdery mildew are obtained through rapid screening; important gene resources and theoretical technologies are provided for breeding new wheat varieties (lines) with ideal germplasm through hybridization means in the future.

The invention provides a treatment response model for acute myelogenous leukemia (AML) accompanied with myelodysplastic syndrome related genetic abnormality (MRGA) and application thereof, and the model combines clinical data, cytogenetics and molecular biology data of patients. Variables significantly related to the complete remission rate of the patient are screened out through Lasso regression and Cox multivariable regression analysis, a treatment response model for predicting the complete remission rate is constructed based on the variables, and a combined scoring system formed by the treatment response model and a constructed survival risk scoring model is used for predicting the response of the patient to induced treatment and 1-3-year survival prognosis. And an individualized basis is provided for clinical treatment decision-making of the AML patient.

The invention discloses a biomarker combination for predicting survival prognosis of an acute myeloid leukemia patient and application of the biomarker combination, and belongs to the technical field of molecular biomedicine. It is found for the first time that the specific N-sugar chain level in a blood sample during definite diagnosis of an AML patient is significantly related to survival prognosis. Through the screened N-carbohydrate chain marker, a Cox regression analysis is utilized to construct an AML prognosis prediction scoring system (AML-GPSS). According to the system, prognosis scores are obtained through a quantitative calculation formula, and patients are divided into different prognosis risk groups according to a preset critical value. The invention provides a brand-new prognosis prediction model based on a protein post-translational modification function, a detection sample is easy to obtain, and the method is stable; an important bioinformatics tool and a new clinical practice scheme are provided for making up for the defects of an existing cytogenetics layering system and achieving early-stage accurate risk layering and individualized treatment decision making of AML patients.

The invention discloses a method for preparing tobacco metaphase chromosomes by utilizing young tobacco plants and application, and belongs to the technical field of preparation of tobacco chromosome specimens. The method comprises the following steps: germinating tobacco seeds at room temperature for 5-7 days, stripping seed shells when the seeds germinate and radicles grow to about 2 mm, sequentially performing N2O treatment on seedlings with radicles, fixing with a stationary liquid, performing enzymolysis in an enzymatic hydrolysate, lightly grinding plant tissues by using a sterile grinding rod until cells are dispersed, centrifuging, airing, adding glacial acetic acid, performing uniform vortex mixing, and performing freeze drying to obtain the tobacco seed suspension. And dripping a small amount of suspension on a clean glass slide to prepare the tobacco metaphase chromosome specimen. According to the method, materials are easy to obtain, the period is extremely short, operation is safe, the chromosome quality is high, and a reliable and efficient standardized scheme is provided for tobacco cytogenetics, molecular cytology and breeding research.

The embodiment of the present application discloses a kind of intelligent chromosome harvesting systems, belong to the technical field of cytogenetics detection.It includes: oscillation module, for executing oscillation operation to test tube;Centrifugal module, for executing centrifugation operation;Liquid extraction module, for extracting supernatant;Image acquisition module, for collecting sample image after oscillation and / or after centrifugation;Intelligent analysis module, for identifying test tube boundary frame, and analyzing image in frame to identify the mixing state and / or centrifugation state of sample;Control module, for: when sample is not mixed, drive oscillation module to repeat oscillation until mixing;When it has been mixed, drive centrifugal module to centrifuge;When sample is not layered, drive centrifugal module to repeat centrifugation or alarm;When it has been layered, drive liquid extraction module to extract supernatant.The present application can realize closed loop oscillation mixing, automatically judge layering and full-process automatic control.

The invention discloses a preparation method of a leukemia bone marrow chromosome specimen, and belongs to the field of biomedicine. According to the preparation method, the chromosome diffusion effect can be effectively improved, and the chromosome morphology is optimized; the repeatability is high, and the personnel operation error is low; and the safety is good, and no open fire is needed. Meanwhile, the success rate and the anomaly detection rate of bone marrow chromosomes are greatly improved. Therefore, the preparation method disclosed by the invention has important significance on leukemia bone marrow chromosome preparation and technical universality, and can effectively improve the value of cytogenetics in the aspects of diagnosis, treatment and prognosis of leukemia.

The invention belongs to the technical field of cell culture, and discloses a bone marrow cell culture medium and a preparation method thereof, and a method for in-vitro culture of bone marrow cells, the bone marrow cell culture medium is composed of a basic culture medium, streptomycin, gentamicin, a platelet lysis buffer, and glutamate enzyme; the final concentration of streptomycin is 50-100 [mu] g / mL, the final concentration of gentamicin is 20-50 [mu] g / mL, the final concentration of platelet lysate is 5-15% (v / v), and the final concentration of glutamate enzyme is 0.1-1 mmol / L. The bone marrow cell culture medium can efficiently support in-vitro proliferation of bone marrow cells, through specific combination and appropriate concentration control of the culture medium, the medium-term cell acquisition amount can be remarkably increased, and medium-term cell chromosomes are complete in morphology and good in dispersity; the standard requirements of clinical bone marrow cell chromosome karyotype analysis, blood disease diagnosis and cell genetics experiments in the scientific research field can be met.

The application discloses a preparation method of leukemia bone marrow chromosome specimens and belongs to the biomedical field. The preparation method can effectively improve chromosome diffusion effect and optimize chromosome morphology, has high repeatability, low personnel operation error, good safety and no need of open fire. Meanwhile, the success rate of bone marrow chromosome and the abnormal detection rate are greatly improved. Therefore, the preparation method has important significance for leukemia bone marrow chromosome preparation and technology popularization, and can effectively improve the value of cytogenetics in leukemia diagnosis, treatment and prognosis.

The present application relates to the technical field of plant chromosome preparation, and specifically discloses a method for increasing the number of mitotic metaphase chromosomes of sugarcane or sugarcane relative genus root tips, comprising: soaking sugarcane or sugarcane relative genus stem double bud segments in an indole-3-acetic acid solution with a concentration of 60-120 mg / L, and culturing in sand or peat substrate; root tip pretreatment: culturing for 2-3 days, selecting healthy and tender root tips, and placing the root tips in saturated p-dichlorobenzene aqueous solution for low-temperature treatment at 8-12 DEG C for 3-5 h; using Carnoy's fixative for fixation; enzymolysis of the root tip growth point; smearing the root tip on a glass slide for preparation; and staining treatment. The method for preparing the present application can achieve the fastest time of only 4 days from root tip culture to chromosome observation, has a short cycle, high efficiency, is convenient and fast for sampling, has a large number of metaphase division phases in the chromosomes, clear morphology, a clean glass slide background, and is convenient for chromosome counting observation and karyotype analysis, and has important significance for the development of sugarcane cytogenetics research.