Preparation method of RBM5 FISH probe for lung cancer detection

A probe and lung cancer technology, applied in biochemical equipment and methods, DNA preparation, microbial measurement/inspection, etc., can solve the problems of high mortality of lung cancer patients, lack of early diagnosis technology, etc., to improve accuracy and safety good, repeatable effect

Inactive Publication Date: 2015-03-25
WUCHANG UNIV OF TECH +1
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  • Abstract
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Problems solved by technology

One of the reasons for the high mortality rate of lung cancer patients is the lack of early diagnosis techniques

Method used

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  • Preparation method of RBM5 FISH probe for lung cancer detection
  • Preparation method of RBM5 FISH probe for lung cancer detection
  • Preparation method of RBM5 FISH probe for lung cancer detection

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Embodiment Construction

[0062] The technical solutions in the present invention will be clearly and completely described below in conjunction with the accompanying drawings in the present invention.

[0063] The invention provides a method for preparing an RBM5FISH probe for lung cancer detection, comprising the steps of:

[0064] Step 1. Screening of clones:

[0065] All clones containing the RBM5 gene were retrieved through the NCBI Mapview database, and these clones were screened to select the optimal clone; the optimal clone containing the RBM5 gene was numbered RP11-493K19.

[0066] Purchasing the clone Life Technology RPCI11.C according to the optimal clone number screened out;

[0067] Step 2. Cultivation of clones:

[0068] Pick the clone RPCI11.C with a toothpick, streak it on the LB solid medium containing 25 μg / ml chloramphenicol, and culture the streaked LB solid medium at 37°C for 16-24 hours to obtain a single colony of RPCI11.C;

[0069] Pick a single RPCI11.C colony and transfer it...

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Abstract

The invention provides a preparation method of an RBM5 FISH probe for lung cancer detection. The RBM5 FISH probe can be applied to early diagnosis of lung cancer, can significantly improve the diagnosis accuracy when combined with an existing molecular probe, is applicable to multiple types of detection samples, such as paraffin sections, cell drops, cell smears and sputum cells, and can be applied to detection of various clinical samples. A kit can be prepared from the RBM5 FISH probe, the RBM5 FISH probe can be applied to fields of tumor biology, cytogenetics and the like, and comprehensive assessment on relations between various molecular markers and development of the lung cancer is facilitated.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for preparing an RBM5 FISH probe for lung cancer detection. Background technique [0002] Lung cancer is one of the most common human malignancies with the highest lethality in the world, and its incidence is on the rise worldwide. One of the reasons for the high mortality of lung cancer patients is the lack of early diagnosis techniques. Scientific research has shown that many lung cancer patients are already in the middle and late stages when they present clinical symptoms, and their 5-year survival rate is less than 15%. However, early diagnosis of lung cancer, especially stage I, will significantly increase the 5-year survival rate of patients to 80%. [0003] The occurrence and development of lung cancer are related to the mutation of a series of genes including EGFR, KRAS, ALK, etc. In recent years, it has been found that RBM5 (RNA-binding motif protein 5) plays an im...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/11C12N15/10
CPCC12Q1/6806C12Q1/6841C12Q1/6886
Inventor 吕家燕代建丽
Owner WUCHANG UNIV OF TECH
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