This invention discloses a
rapid detection method for MNSs
blood typing based on
multiplex digital PCR and a dedicated probe and primer set, belonging to the field of clinical laboratory
medicine and
molecular diagnostics technology. The method involves mixing a PCR reaction mix, the sample to be tested,
enzyme-free
purified water, M / N upstream primer, M / N downstream primer, S / s upstream primer, S / s downstream primer, upstream internal control primer, downstream internal control primer, M probe, N probe, S probe, s probe, and internal control probe to obtain a dPCR
reaction system. This dPCR
reaction system is then added to a
microfluidic chip to generate ≥30,000 homogeneous water-in-oil droplets, followed by PCR amplification. The
fluorescence signal is then detected using a droplet analyzer, achieving accurate and efficient detection of MNSs
blood typing. This method addresses the shortcomings of existing molecular biological detection methods for MNSs
blood typing, such as insufficient specificity, sensitivity, and
throughput, as well as complex operation and high cost, providing reliable
technical support for clinical transfusion safety.