1687 results about "Negative control" patented technology

A disk drive is disclosed comprising a pulse width modulated (PWM) signal generator for generating PWM control signals applied to the driver switches of a voice coil motor (VCM). The PWM control signals comprise a PWM cycle time, a Tforward time interval of the PWM cycle time wherein a positive control voltage is applied to the VCM, a Treverse time interval of the PWM cycle time wherein a negative control voltage is applied to the VCM, and a Tdead time interval of the PWM cycle time wherein a substantially zero control voltage is applied to the VCM. The Tdead time interval is adjusted to control a magnitude of an actual ripple current flowing through the VCM.

An image analysis workstation for analyzing optical thin film arrays is disclosed. One disclosed embodiment relates to individual arrays that comprise a single optical thin film test surface that provides a plurality of discretely addressable locations, each comprising an immobilized capture reagent for an analyte of interest. These are referred to herein as "arrayed optical thin film test surfaces." Preferably, an individual arrayed optical thin film test surface comprises at least 4, more preferably at least 16, even more preferably at least 32, still more preferably at least 64, and most preferably 128 or more discretely addressable locations. One or more of the discretely addressable locations may provide control signals (e.g., for normalizing signals and / or that act as positive and / or negative controls) or fiducial signals (i.e., information that is used to determine the relative alignment of the arrayed optical thin film test surface within the device.

Vector compositions comprising a myeloproliferative sarcoma virus enhancer, negative control region deleted, dl587rev primer-binding site substituted (MND) promoter operably linked to a chimeric antigen receptor (CAR) are provided.

A method for inspecting hepatitis and AIDS nucleic acid by synchronized amplification and its reagent knit are disclosed. The process is carried out by taking magnetic ball as automatic medium, specific synchronized capturing HBV, CHV and HIV nucleic acid, accelerating biotin primer construction and purification by RNA external label and internal label, real-time synchronized inspecting and T-PCR amplifying based on Tagman probe. The reagent knit consists of dis-inhibitor, cracking liquid, magnetic ball suspension, washing liquor, internal check, RT-PCR reactive liquor, enzyme mixture, fluorescent mixture, positive check and negative check. It's accurate and automatic, has single-tube operation, closed inspection AND synchronized extraction, and it has better sensitivity and specific performance and can be used for large-scale blood screening and large-capacity clinical inspection.

Adaptive rail power amplifier technology processes an audio signal by feeding the audio signal to the power amplifier to produce an output signal, applying positive and negative power supply voltages centered with respect to the audio signal to the positive and negative power supply rails of the power amplifier, comparing the output signal with the positive and negative power supply rail voltages to produce dynamically varying positive and negative control signals, feeding the positive and negative control signals to positive and negative high current charge pumps and adding supplemental positive and negative voltages from the positive and negative charge pumps to the positive and negative power supply rails to produce a linear adaptive rail voltage which tracks the output signal.

The invention discloses a fluorescence quantitative PCR detection kit of hepatitis B virus and an application thereof. The kit is composed of the following independent components: DNA extraction solution I, DNA extraction solution II, DNA extraction solution III, DNA extraction solution IV, positive control interior label, PCR reaction liquid, probe HBV-SP, enzyme mixed liquor containing heat resistant DNA polyase and uracil DNA glycosylase, quantitative hepatitis B virus reference material, hepatitis B virus positive control serum and hepatitis B virus negative control serum, wherein DNA extraction solution I contains 0.2-1.0% of lauryl sodium sulphate (mass / volume), 1.0-4.0% of Triton (volume / volume) and 0.2-1.0mol / L of guanidinium isothiocyanate; DNA extraction solution II contains 100-300mmol / L of 4-HEPES, 100-300mmol / L of sodium chloride with pH of 6.5+ / -0.2 and 100-400 mu g / ml of magnetic beads; DNA extraction solution III contains 0.1-1.0% of Triton (volume / volume) and 100-300mmol / L of sodium chloride; DNA extraction solution IV contains mineral oil. The fluorescence quantitative PCR detection kit of hepatitis B virus of the invention can be used for detecting the HBV-DNA concentration in samples of serum, blood plasma or latex and the like.

The invention discloses a multi-target-point CRISPR / Cas9 expression vector based on bacteriostasis and sterilization. The gene sequence of the multi-target-point CRISPR / Cas9 expression vector is SEQ ID NO1 and the gene sequence of a Cas9 expression vector is SEQ ID NO2. A construction method of the expression vector comprises: using a DNA segment of an oriented RNA specific recognition gene, mediating Cas9 protein to cut a DNA double strand to produce a double strand incision and break a DNA sequence, and therefore breaking the principle of DNA coding of functional protein to construct the multi-target-point CRISPR / Cas9 expression vector. Through comparison with a negative contrast, after CRISPR / Cas9 acts on DNA gyrase and dihydrofolate reductase, the survival rate of bacteria is only 10%, and the sterilization effect can reach no less than 90%.

The invention discloses a security certification method and a security certification device for power grid equipment and a negative control terminal. The method comprises the following steps: receiving electric power information comprising security certification data and instruction data and sent by master station equipment in a power grid; performing security certification on the power grid equipment according to the security certification data to generate a security certification result; and executing the operation corresponding to the instruction data according to the security certification result. The invention also discloses the security certification device for the power grid equipment. The device comprises an information receiving unit, a security certification unit and an instruction execution unit, wherein the information receiving unit is used for receiving the electric power information comprising the security certification data and the instruction data and sent by the master station equipment in the power grid; the security certification unit is used for performing security certification on the power grid equipment according to the security certification data to generate the security certification result; and the instruction execution unit is used for executing the operation corresponding to the instruction data according to the security certification result. Simultaneously, the invention also discloses the negative control terminal.

The invention discloses a monoclonal antibody blocking enzyme-linked immunosorbent assay (ELISA) kit and a method for detecting the nonstructural protein (NSP) antibody of a foot-and-mouth disease virus (FMDV) (FMD NSP B-ELISA); the kit comprises ELISA reaction plates, serum diluent, 25 times concentrated detergent, substrate solution, 100* concentrated ELISA detecting antibody, stop buffer, positive control serum and negative control serum; the ELISA reaction plates are two 96-pore high-affinity ELISA reaction plates, firstly 6* groups of amino acid monoclonal antibody or NSP 2C polyclonal antibody, and then FMDV 3ABC or 2C3AB NSP which is expressed by pronucleus and is provided with 6* groups of amino acid labels is captured through the monoclonal antibody or the polyclonal antibody; and compared with other similar kits, the method has higher coincidence rate and higher positive serum detection rate, and is applicable to detecting the serum of cattle, sheep, pigs and other susceptible animals.

The present invention provides a microporous membrane for detecting at least one target analyte in a sample. The membrane includes an array that comprises at least one capture element and the at least one control element printed on the membrane surface, the at least one capture element corresponding to and being able to bind a target analyte, the plurality of control elements, when present including: i) at least one fiduciary marker, ii) at least one negative control to monitor background signal, iii) at least one negative control to monitor assay specificity, iv) at least one positive colorimetric control, v) at least one positive control to monitor assay performance or any combination thereof.

A method for calculating distances between stimulus response curves (e.g., dose response curves) allows classification of stimuli. The response curves show how the phenotype of one or more cells changes in response to varying levels of the stimulus. Each “point” on the curve represents quantitative phenotype or signature for cell(s) at a particular level of stimulus (e.g., dose of a therapeutic). The signatures are multivariate phenotypic representations of the cell(s). They include various features of the cell(s) obtained by image analysis. To facilitate the comparison of stimuli, distances between points on the response curves are calculated. First, the response curves may be aligned on a coordinate representing a separate distance, r, from a common point of negative control (e.g., the point where no stimulus is applied). Integration on r may be used to compute the distance between two response curves. The distance between response curves is used to classify stimuli.

The invention discloses a male multi-tumor marker detection protein chip and a kit thereof. The chip comprises a substrate, protein markers distributed in an array type and point coatings of contrasts, wherein the substrate is a glass substrate or a film substrate; and the tumor markers and the point coatings of the contrasts are seven protein markers of AFP, CEA, NSE, CYFRA21-1, CA19-9, tPSA and SCC-ag, a positive contrast and a negative contrast which are uniformly distributed and latticed on the substrate. A reaction result of various indexes can be obtained only through one reaction by utilizing the protein chip, and the following tumors can be simultaneously screened: primary liver cancer, prostatic cancer, pancreatic cancer, lung cancer, esophageal cancer, gastric cancer and colorectal cancer. The invention is particularly suitable for the general examination of malignant tumors of male asymptomatic groups and high risk groups.

The invention provides a pump controller for a construction machine that allows an operator to set the maximum speed of a specific actuator arbitrarily without altering the negative control and acquire a smooth operation. The pump controller comprises a plurality of control valves to supply actuators with a controlled pressure oil, a variable displacement hydraulic pump to supply the plural control valves with the pressure oil in parallel, a center bypass connected in parallel to the control valves, and a restrictor to generate a control pressure for negatively controlling the variable displacement hydraulic pump installed at a terminal end of the center bypass. The pump controller further comprises pressure sensors to recognize the operation of the actuator, a controller that sets a pump discharge characteristic individually to the control valve for the actuator in accordance with the operation state of the actuator, calculates a pump discharge corresponding to the control pressure using the set pump discharge characteristic, and controls the angle of inclination of a regulator.

The invention discloses an electric wireless private network channel access control method based on service classification. The method is characterized in that an electric wireless private network comprises a master station computer, a front-end machine, a base station and data collection terminals, the data collection terminals are used for achieving electric negative control information collection, the data collection terminals are connected with the base station in a wireless self-organization network mode, the base station is connected with the master station computer through a wired link, messages are divided into at least six kinds in a wireless self-organization network formed by the data collection terminals and the base station according to service types, the message of each type corresponds to one priority level, and different waiting time intervals exist between the messages of different types in the channel access process according to the sequence of the priority levels. According to the method, the information transmission capacity of the electric wireless private network can be improved, and the real-time performance and reliability of emergency messages can be guaranteed.

The invention is an image processing-based dynamic target auto zooming system, mainly comprising: variable focal lens to regulate focus of camera; camera to shoot target image; image processing control unit to receive image data from the camera and divide the target to obtain image size of the target, and determine to send control command to the lens drive control circuit according to the comparative result of target size and set size; lens drive control circuit connected with the image processing control unit through serial interface and connected with positive and negative control lines of the variable focal lens through two cables and receiving the control command from the image processing control unit to shift the variable focal lens so as to regulate the focus of the camera. And the invention has auto zooming function and advantages of high efficiency and high accuracy.

A differential line driver includes a plurality of driver cells. Control logic outputs positive and negative control signals to the driver cells so as to match a combined output impedance of the driver cells at (Vop, Von). Each driver cell includes an input Vip and an input Vin, an output Vop and an output Von, a first PMOS transistor and a first NMOS transistor having gates driven by the input Vip, and a second PMOS transistor and a second NMOS transistor having gates driven by the input Vin. A source of the first PMOS transistor is connected to a source of the second PMOS transistor. A source of the first NMOS transistor is connected to a source of the second NMOS transistor. First and second resistors are connected in series between the first PMOS transistor and the first NMOS transistor, and connected together at Von. Third and fourth resistors are connected in series between the second PMOS transistor and the second NMOS transistor, and connected together at Vop. A first output switch is driven by a corresponding positive control signal and connected between a supply voltage and the sources of the first and second PMOS transistors. A second output switch driven by a corresponding negative control signal and connected between a ground and the sources of the first and second PMOS transistors.

The device comprises: a sample application zone, a reagent zone, a detection zone with positive and negative contrast zones and the combination zone for detected target, and a reagent strip. Wherein, the positive contrast zone comprises one or more material to show the first color on dried condition and the second color when on wet condition. If there is detected target in sample, the interactive action of the combination zone and contrast zone forms the legible symbols.

The invention provides a kit for jointly detecting respiratory tract pathogen through a multiple fluorescent PCR method. The kit comprises six components: reaction liquid A, reaction liquid B, reaction liquid C, enzyme mixed liquid, positive control and negative control, and comprises 11 common respiratory tract pathogen detections (general type of influenza virus A, influenza virus B, respiratory syncytial virus, 1 / 2 / 3 type of human parainfluenza virus, adenovirus, mycoplasma pneumoniae, chlamydia pneumonia, legionella pneumophila, streptococcus pneumonia, haemophilus influenza, A streptococcal); the amplification is performed through three reaction buffers, and each reaction buffer contains four fluorescent channels, 90% pathogen infection on the clinic can be checked.

The invention relates to a respiratory tract common pathogen multiple PT-PCR combined gene chip detection kit. The kit detects one or more of 22 respiratory tract common pathogens by the usage of multiple specific conservative degenerate primer combination and probe combination and provided with endogenous control, positive control and negative control at the same time. According to the detection kit, the coverage rate for a high mutant pathogen subject sequence is increased, the problem of nonspecific cross reaction between the multiple primers and the probe is avoided, one single reaction system can detect more than 20 respiratory tract common pathogens simultaneously, and a detection tool which is simple and sensitive, rapid and large in flux and capable of carrying out multi-index parallel detection is provided for respiratory tract common pathogen detection.

The invention discloses a processing method of fluorescence intensity data in fluorescence droplet detection. The method is used for the nucleic acid detection based on fluorescence droplet. The method comprises the steps of step 1, acquiring the fluorescence intensity data of all fluorescence droplets, and preprocessing the data, step 2, conducting a primary classification of the data, step 3, based on the result of the primary classification, acquiring the fluorescent intensity distribution of the negative droplets and the positive droplets, step 4, calculating final decision threshold value t, step 5, using the decision threshold value t for a second time classification of data, step 6, calculating a sample concentration. The processing method accurately describes the distribution of the fluorescent intensity of digital PCR fluorescent micro-drop, and can automatically determine the suitable threshold value automatically to identify the quantity of the positive micro-droplets without the need of negative control. The processing method can effectively enhance the accuracy of data classification, thus considerably enhancing the accuracy of the fluorescence droplet detection result.

Apparatus, methods, and computer programs for semiconductor processing in a capacitively-coupled plasma chamber are provided. A chamber includes a bottom radio frequency (RF) signal generator, a top RF signal generator, and an RF phase controller. The bottom RF signal generator is coupled to the bottom electrode in the chamber, and the top RF signal generator is coupled to the top electrode. Further, the bottom RF signal is set at a first phase, and the top RF signal is set at a second phase. The RF phase controller is operable to receive the bottom RF signal and operable to set the value of the second phase. Additionally, the RF phase controller is operable to track the first phase and the second phase to maintain a time difference between the maximum of the top RF signal and the minimum of the bottom RF signal at approximately a predetermined constant value, resulting in an increase of the negative ion flux to the surface of the wafer.

The invention discloses an indirect ELISA diagnosing reagent box for pig II type circular virus (PCV2) antibody. There arranged with antibody detecting board in the reagent box, enzyme combination liquid, positive comparison, negative comparison, sample diluter, 10X condensed detergent, developing liquid A and B, and stopping liquid, the detecting board of the reagent box is removable 96-apertures enzyme labeling board enclosed with PCV2 reconstructed enucleation localizing signal capsid protein (dCap), the enzyme combination liquid is goat-anti-pig IgG multi-clone antibody labeled with HRP, the positive comparison is pig PCV 2 standard positive serum, the negative comparison is pig standard negative serum. The sensitivity of the invention is high, simple, and easy to be wide applied.

The invention relates to a kit for utilizing a magnetic bead-RNA concentrating technology to extract purified target RNA as well as testing chlamydia trachomatis (CT) by using constant temperature nucleic acid simultaneous amplification detection technology (SAT). The kit comprises urine sample preservation solution, nucleic acid extracting solution, cleaning solution, CT reaction solution, CT detection solution, STA enzyme liquid, CT positive control and CT negative control. The kit has high specificity and sensitivity; furthermore, the amplified product RNA is easy for degradation in natural environment with little pollution.

The invention belongs to the technical field of immunologic diagnosis, in particular to a treponema pallidum antibody diagnostic kit by a chemiluminescence method and a preparation method thereof. The kit comprises an anti-TP test reaction plate, an anti-TP test enzyme complex, chemiluminescence substrate liquid, concentrated washing liquor, a negative contrast and a positive contrast. The invention also discloses a preparation method of the diagnostic kit, which adopts a chemiluminescence immunoassay technology; compared with ELISA (enzyme-linked immuno sorbent assay), the method has higher sensitivity and specificity, is suitable for the auxiliary diagnosis of clinical syphilis and screening of blood donors and fills a blank of the production of a treponema pallidum antibody diagnostic reagent detected by the domestic chemiluminescence method.

The invention provides an immunity diagnosis test kit for detecting II-type dengue virus antigen, which comprises a porous reaction plate covering monoclonal antibody DV2-M6, a sample treatment liquid, a monoclonal antibody DV2-M15 marked with a label, a positive contrast, a negative contrast, a concentration washing liquid, a develop liquid and a termination liquid, wherein the monoclonal antibodies DV2-M6 and DV2-M14 of the test kit can be specifically combined with NS1 protein of II-type dengue virus, without cross reaction with other three kinds of serotype dengue viruses NS1 and respectively combined with different antigen points of NS1, while the check sensitivity of NS1 protein of II-type dengue virus can reach 3ng / ml and the check sensitivity of culture supernatant of II-type dengue virus infection cell is 8 power of Pan-E dengue early elisa test kit, thereby improving the sensitivity of clinical serum sample check.

The invention discloses an enzyme-linked immunoassay kit of a structural protein antibody for a seneca valley virus. The kit comprises an elisa plate, positive control serum, negative control serum, an HRP-conjugated antibody, a sample diluent, a 20-fold concentrated detergent, a substrate solution A, a substrate solution B and a stop solution, wherein the elisa plate is coated with a structural protein epitope polypeptide composition for the seneca valley virus. The epitope polypeptide composition is one or any combination of more than two of a polypeptide as shown in a sequence 1, a polypeptide as shown in a sequence 2, a polypeptide as shown in a sequence 3 or a polypeptide as shown in a sequence 4 in the sequence table. The elisa plate is coated with a chemical synthetic antigen peptide, so that the kit is low in antigen dosage and high in sensitivity and specificity, and whether the structural protein antibody is infected by the seneca valley virus or not can be efficiently detected. The kit is high in sensitivity, good in specificity, convenient in operation, and has a good market prospect.

The invention discloses a pig mycoplasma pneumonia recombination antigen ELISA detection Kit. The Kit is provided with an antibody detection plate, enzyme conjugate treatment fluid, a positive control, a negative control, sample diluent, 10x condensed cleaning solution, developing solution A, developing solution B and termination solution. The detection plate of the Kit is a detachable 96-pore enzyme label plate enveloped by the mutational pig mycoplasma pneumonia membrane protein P46 gene protein antigen, the enzyme conjugate treatment fluid is a rabbit anti-pig antibody labeled by horse radish peroxidase, the positive control serum is taken from a pig which is detected positive through indirect hemagglutination and the ELISA Kit of IDEXX and has obvious pig mycoplasma pneumonia lesions in the lungs after anatomy, and the negative control serum is taken from a pig which is detected negative through indirect hemagglutination and the ELISA Kit of IDEXX and has no pig mycoplasma pneumonia lesions in the lungs after anatomy. The pig mycoplasma pneumonia recombination antigen ELISA detection Kit has the advantages of strong specificity, high sensitivity, simple operation, easy large-scale generation and application, and broad market prospect.

The invention discloses a blocking ELISA kit for detecting an NDV (Newcastle disease virus) antibody. The blocking ELISA kit for detecting the NDV antibody comprises an ELISA plate coated with an NDV inactivated antigen, an NDV positive control serum, an NDV negative control serum and a horseradish peroxidase labeled NDV NP protein monoclonal antibody, wherein the horseradish peroxidase labeled NDV NP protein monoclonal antibody is secreted by a hybridoma cell strain with the preservation number being CCTCC NO: C2016180. The blocking ELISA kit for detecting the NDV antibody can detect serum samples which are infected with the suspected NDV and are from different species, can distinguish an MG7-deficient vaccine from an NDV serum after being infected with a wild virus, and has no cross reaction with a common avian viral pathogen positive serum, thereby being high in sensitivity and specificity, good in reproducibility and suitable for high-throughput detection of serum samples.

The invention discloses a toxoplasma gondii IgM antibody detection kit combined with the FITC-anti-FITC indirect coating technology and the chemiluminescent immunoassay technology, and a preparation method thereof. The kit of the invention is composed of a negative control, a positive control, solid-phase vectors for anti-FITC antibodies, anti-human Mu-chain monoclonal antibodies of FITC markers, toxoplasma gondii antigens which are marked by horse radish peroxidase, chemiluminescent substrates and concentrated washing solutions. The kit of the invention can be used as the aided detection index for prenatal prepotency diagnosis, and has vital significances for improving the birth population quality and doing the family planning and the prepotency well.