7 results about "Negative control" patented technology

The present disclosure relates to methods of characterizing a system comprising a chromatography column. The methods can comprise introducing a sample comprising a positive control and a negative control into a system comprising a chromatography column, wherein the positive control is a sensitive probe that interacts with the system and the negative control does not substantially interact with the system; detecting the positive control and the negative control after passing the sample through the chromatography column; and determining system suitability by comparing the amount of positive control detected to the negative control. In some embodiments, determining system suitability (e.g., sample inertness to the system) is accomplished by determining the ratio of positive control to negative control detected.

The application discloses a kind of negative control equipment control method and device, when not receiving the detection data sent by negative control equipment in preset time, negative control equipment is determined as offline equipment, and the target electric quantity value corresponding to offline equipment is obtained, the running state of offline equipment is determined according to the detection data and target electric quantity value last sent by offline equipment, and the disposal scheme of offline equipment is determined according to running state, and the equipment state of offline equipment is adjusted according to disposal scheme.The technical problem that the prior art only directly restarts negative control equipment when negative control equipment is offline, and the reason for the drop of negative control equipment is not analyzed, resulting in the reliability of power system operation is reduced.

The invention provides a domain name resolution negative control method based on object storage and a related device, the method is applied to the field of object storage, and the method comprises the following steps: a domain name resolution module in an object storage service node receives an access request, the access request carries a bucket name of a storage bucket, and the access request is used for requesting position information of the storage bucket; according to the bucket names of the storage buckets, under the condition that the position information of the storage buckets does not exist in a pre-judgment database, the number of access requests in a first queue is counted, the first queue is used for storing the access requests which are pre-judged to have no position information of the storage buckets, if the number exceeds a flow control threshold value, a default response is returned, and if the number does not exceed the flow control threshold value, a default response is returned; and adding the access request into a first queue, wherein the access request in the first queue is used for further determining whether the position information of the storage bucket exists or not. By implementing the method provided by the invention, attacks of invalid buckets can be effectively reduced or blocked, so that the object storage service node can process normal businesses without being influenced.

A method for controlling a braking force of a brake system of a vehicle by means of a control device is provided. The method comprises the following steps: if there is a positive control difference between a braking force setpoint value (Bremskraft_Set) and a braking force actual value (Bremskraft_Ist): determining a first torque setpoint value (Drehmoment_Set 1); inputting the torque setpoint value as a manipulated variable (Drehmoment_Set) into a controlled system (14) of the brake system; and adjusting the braking force on the basis of the input manipulated variable (Drehmoment_Set); if there is a negative control difference between the braking force setpoint value and the braking force actual value after a preceding positive control difference: determining a second torque setpoint value (Drehmoment_Set 2) from the control difference; calculating a compensated manipulated variable (Drehmoment_Set) by subtracting a hysteresis value (Drehmoment_Hyst) from the second torque setpoint value (Drehmoment_Set 2); inputting the compensated manipulated variable (Drehmoment_Set) as the manipulated variable into the controlled system (14) of the brake system; and adjusting the braking force on the basis of the input compensated manipulated variable (Drehmoment_Set).

The present invention discloses a rapid identification kit for Salmonella pullorum and Salmonella gallinarum based on the mod gene. The kit includes detection primers, a positive control, a negative control and a blank control. The positive control is Salmonella pullorum ATCC9184 genomic DNA, the negative control is Salmonella pullorum ATCC10398 genomic DNA, and the blank control is sterile double-distilled water. The primers include: For: 5'-TGTTCGGTCGGTTCTTTATAGGA-3'; Rev: 5'-CGGAGATAATCTGGATGTGTTACG-3'. The present invention also discloses a PCR method for rapidly identifying two biotypes of Salmonella pullorum and Salmonella gallinarum in Salmonella gallinarum serotypes using the mod gene as a detection target. The method of the present invention has the advantages of being rapid, simple, highly specific and highly sensitive. Compared with traditional biochemical identification methods for Salmonella pullorum and Salmonella gallinarum, it has great advantages in detection time and detection cost, and is suitable for clinical batch detection.

The kit for detecting KRAS and NRAS gene mutation is characterized by comprising a KRAS detection reaction solution, an NRAS detection reaction solution, a KRAS positive control, an NRAS positive control, a negative control and a blank control, according to the present invention, based on the high multiplex PCR (Polymerase Chain Reaction) and the high precision capillary electrophoresis method, the kit for detecting the KRAS and NRAS gene mutation is developed, wherein the kit has characteristics of high sensitivity and high detection flux. The kit provided by the invention takes FFPE sample human genome DNA as a detection sample, and can be applied to preparation of a detection reagent by detecting KRAS and NRAS gene mutation. The method is high in sensitivity (can reach 0.5%), detection can be completed only in 150 min, and meanwhile the method has the advantages of being good in specificity, high in accuracy, low in price and easy to operate.

The invention provides an online car-hailing travel processing method and device, electronic equipment, a computer readable storage medium and a computer program product, and relates to the technical field of computers, the field of Internet of Vehicles and the technical field of artificial intelligence, and the method comprises the steps: responding to the ending of the online car-hailing travel, and displaying an appeal entrance for the online car-hailing travel; in response to a trigger operation for the appeal entrance, displaying an appeal determination interface which is used for determining the fee of the online car-hailing travel; wherein the appeal determination interface comprises a negative control, and the negative control is used for indicating the fee of the negative online car-hailing travel; in response to a triggering operation aiming at the negative control, when the online car-hailing travel meets a first fee exempting condition, displaying fee exempting information; wherein the expense removal information is used for indicating that at least part of expense of the online car-hailing travel is removed. According to the invention, the efficiency of processing the online car-hailing travel can be improved.