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Treponema pallidum antibody diagnostic kit and preparation method thereof

A technology of Treponema pallidum and diagnostic kit, applied in the field of immunodiagnosis, to achieve high sensitivity and good specificity

Active Publication Date: 2010-06-16
威海威高生物科技有限公司
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  • Abstract
  • Description
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  • Application Information

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Problems solved by technology

However, at present, there is no chemiluminescence diagnostic reagent produced for the detection of Treponema pallidum antibody in China. At present, there is an urgent need for a chemiluminescent diagnostic reagent with high sensitivity and specificity, which can be used as a substitute for ELISA reagents and other reagents for the diagnosis of syphilis.

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  • Treponema pallidum antibody diagnostic kit and preparation method thereof
  • Treponema pallidum antibody diagnostic kit and preparation method thereof

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Embodiment Construction

[0015] The kit of the invention adopts the chemiluminescent immunoassay technology to detect whether there is Treponema pallidum antibody in the serum. This example specifically describes the Treponema pallidum antibody diagnostic kit and its preparation method. A Treponemal pallidum antibody diagnostic kit, which includes an anti-TP reaction plate, an anti-TP enzyme conjugate, a chemiluminescence substrate solution, a concentrated washing solution, a negative control and a positive control, the anti-TP reaction plate 48 wells or 96 wells, loaded with Treponema pallidum-specific recombinant antigens TP15, TP47; 1 bottle of anti-TPase conjugate, containing recombinant antigens TP15, TP47 and horseradish peroxidase synthesized by sodium periodate method Marker; 2 bottles of chemiluminescence substrate solution, which is chemiluminescence substrate solution A solution: 0.2M pH7.8 Tris-Hcl, 2.0g / L luminol, 0.5g / L sodium tetraphenylborate, chemiluminescence substrate Solution B: 0...

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Abstract

The invention belongs to the technical field of immunologic diagnosis, in particular to a treponema pallidum antibody diagnostic kit by a chemiluminescence method and a preparation method thereof. The kit comprises an anti-TP test reaction plate, an anti-TP test enzyme complex, chemiluminescence substrate liquid, concentrated washing liquor, a negative contrast and a positive contrast. The invention also discloses a preparation method of the diagnostic kit, which adopts a chemiluminescence immunoassay technology; compared with ELISA (enzyme-linked immuno sorbent assay), the method has higher sensitivity and specificity, is suitable for the auxiliary diagnosis of clinical syphilis and screening of blood donors and fills a blank of the production of a treponema pallidum antibody diagnostic reagent detected by the domestic chemiluminescence method.

Description

technical field [0001] The invention relates to an immunodiagnostic technique, in particular to a Treponema pallidum antibody diagnostic kit and a preparation method thereof. Background technique [0002] We know that syphilis (Syphilis) is a chronic systemic infectious disease caused by Treponema pallidum (Treponema pallidum). It is one of the classic venereal diseases, and sexual contact transmission accounts for 95%. Infectious diseases are currently one of the top ten infectious diseases in my country. The clinical manifestations of this disease are complicated, the course of the disease is long, and it can invade almost every tissue and organ of the whole body. Syphilis has been basically eliminated in my country in 1959, but since the 1970s, after the reform and opening up, with the development of society and the increase of exchanges with foreign countries, sexually transmitted diseases have come back to life in our country, especially the number of syphilis cases has...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/571
Inventor 蒙红胜贾向阳贾毅周雷赵海英
Owner 威海威高生物科技有限公司
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