47 results about "Enzyme complex" patented technology

The invention discloses a multi-enzyme compound noodle stretching agent and application thereof, and belongs to the technical field of food additives. The multi-enzyme compound noodle stretching agent is prepared from the following components in parts by mass: 1 to 10 parts of papain, 1 to 10 parts of trypsin, 1 to 10 parts of glucose oxidase, 1 to 10 parts of lipase and 10 to 30 parts of tea polyphenol. Through the synergistic effect of multienzyme compounding and tea polyphenol, the dough structure and the noodle processing performance are improved, the protein depolymerization and crosslinking degree and the protein oxidation degree of the dough can be regulated and controlled at the same time, the dough strength and ductility are considered, the cooking loss is reduced, meanwhile, the green and natural characteristics are kept, no chemical modifier is added, and the production cost is reduced. And the limitation of the existing single enzyme or chemical modifier is overcome, so that the method has a relatively good application prospect in the technical field of food additives.

The invention belongs to the technical field of synthetic biology and enzyme engineering, and particularly relates to a multi-enzyme in-vivo space co-localization carbon metabolic flux strengthening method, a genetically engineered bacterium and application of the genetically engineered bacterium. According to the invention, a recombinant expression vector is constructed, key enzymes RHLA and RHLB participating in synthesis of a target product are respectively subjected to fusion expression with protein scaffold modules RIAD and RIDD through (G4S) 3 flexible connecting peptide, and are introduced into host chassis bacteria, so that a stable multi-enzyme composite system is formed in cells, the transmission distance of a metabolic intermediate is shortened, and the expression efficiency is improved. Spatial co-localization and concerted catalysis of key catalytic enzymes are realized, and the conversion efficiency of a carbon source to a target product is improved. Compared with the prior art, the genetically engineered bacterium constructed by the invention has the advantages of stable structure, no need of complex regulation, reasonable metabolic flow distribution and the like in the fermentation process, the synthesis efficiency and yield of the target biosurfactant are remarkably improved, and the feasibility of industrial amplification application is enhanced.

This invention discloses a lipophilic yeast strain for synthesizing astaxanthin (Yersinia lipophila). Yarrowia lipolytica This invention relates to an engineered strain, its construction method, and its application. The engineered strain is a *Yersinia lipolytica* strain carrying CrtZ and CrtW. The invention utilizes genetic engineering methods to introduce a *Yersinia lipolytica* strain derived from *Rhodochophora* into a β-carotene-producing strain via genetic engineering. Haematococcus Pluvialis The β-carotene hydroxylase encoding gene (HpCrtZ) originates from the genus Paracoccus ( Paracoccus sp. The gene encoding β-carotene ketolase (PsCrtW) and the gene derived from Saccharomyces cerevisiae (Saccharomyces cerevisiae) Saccharomyces cerevisiae The method involves obtaining a genetically engineered *Yersinia lipolytica* strain that produces astaxanthin by constructing a fusion enzyme complex and fusing it with peroxisomes, endoplasmic reticulum, and lipid droplet-targeting signal peptides to obtain a *Yersinia lipolytica* strain with higher astaxanthin yield. The astaxanthin-producing genetically engineered strain constructed in this invention achieved an astaxanthin yield of 1.43 g / L in a 5 L bioreactor after pH control and continuous feeding optimization.

The invention discloses a multi-enzyme complex of S-adenosylmethionine decarboxylase / spermine synthetase and an application of the multi-enzyme complex in production of spermine. Belongs to the technical field of enzyme engineering. The purpose of the present invention is to increase the yield of spermine. The invention provides a multi-enzyme complex of S-adenosylmethionine decarboxylase / spermine synthetase. The multi-enzyme complex is obtained by connecting the S-adenosylmethionine decarboxylase and the spermine synthetase through a flexible linker. And a theoretical basis is provided for preparation of spermine.

The invention provides a controllable photo-thermal-enzymatic degradation porous material as well as a preparation method and application thereof. The controllable photo-thermal-enzymatic degradation porous material is prepared from the following raw materials: an oil phase mixture and a water phase mixture, the oil phase mixture comprises an enzyme sensitive polymer base material, a random heteropolymer (RHP)-enzyme compound, Janus photo-thermal nanoparticles, an internal phase dispersing agent and a solvent; the aqueous phase mixture comprises water, electrolyte and alginate. According to the porous material provided by the invention, the Janus photo-thermal nanoparticles and the RHP-enzyme compound are coupled, under near-infrared light, RHP conformational change can be triggered to release BC lipase, degradation of the porous material can be triggered under the mild condition of 37 DEG C, and the degradation rate within 48 hours can reach 98%.

This application discloses a multi-enzyme complex based on a Snap-Fit self-assembly tag, its construction method, and its applications, relating to the field of protein engineering technology. The multi-enzyme complex based on the Snap-Fit self-assembly tag comprises: a TLL-FitC fusion protein with the amino acid sequence shown in SEQ ID NO.13, a FitN-AnChOx fusion protein with the amino acid sequence shown in SEQ ID NO.14, and an OleT protein with the amino acid sequence shown in SEQ ID NO.15. PRN - SnapG fusion protein. This multi-enzyme complex employs a Snap-Fit assembly strategy, which allows three different enzymes to be assembled using only one set of tags. When applied to the synthesis of olefins, it helps to improve the transfer efficiency of 1-olefin cascade reaction intermediates between upstream and downstream enzyme active sites, thereby increasing the final yield, and has great application potential.

The invention discloses a multistage enzyme and quorum sensing signal molecule MABR membrane and a preparation method thereof, through a bionic induction strategy, while the anti-pollution characteristic of the MABR membrane is maintained, multistage enzyme and quorum sensing signal molecules are fixed on the surface of the membrane, and the multistage enzyme and quorum sensing signal molecules induce microorganisms to secrete an extracellular polymeric substance (EPS), so that the binding strength of a biological membrane is improved by 40% or more. The preparation method of the multi-stage enzyme and quorum sensing signal molecule MABR membrane comprises the following steps: (1) screening nitrifying bacteria and extracting multi-stage enzyme and quorum sensing signal molecules of the nitrifying bacteria; (2) carrying out composite pretreatment on the surface of the MABR membrane, wherein the composite pretreatment comprises hydrophilic modification, functional group introduction, swelling of the surface of membrane silk and the like; and (3) immobilizing multistage enzyme and quorum sensing signal molecules (including cell membrane fragments, periplasmic space enzyme complexes and the like) in the strain by adopting an immobilization technology to form a bioactive interface layer with the thickness of about 50-200nm.

The application discloses a method for constructing a multi-enzyme complex engineering strain, and has the characteristics that the method comprises the following steps: S1, constructing a light decarboxylase FAP and an oil-producing yeast fatty acid synthase fusion expression vector; S2, electrically shocking and transforming the constructed target vector into an agrobacterium competent AGL1, and introducing the target vector into a starting strain through an agrobacterium-mediated transformation method to obtain an engineering strain that is back-supplemented with a fatty acid defect; the engineering strain that is back-supplemented with the fatty acid defect is obtained, the FAP-FAS2 fusion expression is realized at the same time as the self-assembly of the intracellular FAS1, and a FAP-FAS synthase complex is obtained. The complex not only back-supplements the fatty acid defect function, so that the strain grows normally, but also can synthesize alkanes in the process of blue light irradiation fermentation culture, and realizes the intracellular assembly of protein macromolecules.

The invention relates to a rhodium nano-enzyme compound and application thereof in preparation of drugs for preventing, relieving and / or treating drug-induced liver injury. The rhodium nano-enzyme compound comprises an extracellular vesicle simulant and rhodium nano-enzyme located in the extracellular vesicle simulant. The surface of the rhodium nano-enzyme is coated with the extracellular vesicle simulant, the extracellular vesicle simulant has good biocompatibility and low immunogenicity and can promote tissue repair and regeneration, and the extracellular vesicle simulant can be removed in a non-specific mode and then is enriched in the liver in a targeted mode, so that the rhodium nano-enzyme is enriched in the liver together with the extracellular vesicle simulant. The rhodium nano-enzyme has excellent simulation activity of superoxide dismutase (SOD) and catalase (CAT), can efficiently remove various active oxygen species, and can inhibit inflammatory reaction caused by APAP, so that the rhodium nano-enzyme has a targeted treatment effect on drug-induced liver injury.

The invention relates to the field of bio-enzyme catalysis, and discloses a bio-enzyme catalysis process for reducing agent modification, which comprises the following steps: S1, enzyme solution preparation: mixing a bio-enzyme preparation with a buffer solution with the pH value of 5-8 to prepare an enzyme solution with the enzyme concentration of 0.5-5.0 g / L; the biological enzyme preparation is a compound enzyme composed of one or more of cellulase, hemicellulase and lignin degrading enzyme; s2, enzymolysis modification: mixing a carbonaceous reducing agent raw material with the enzyme solution prepared in the step S1 according to a mass ratio of 1: 5-1: 20, carrying out an enzymolysis reaction at 40-60 DEG C, and meanwhile, applying ultrasonic waves to carry out auxiliary treatment; the power of the ultrasonic waves is 100-500 W, and the frequency of the ultrasonic waves is 20-40 kHz And S3, solid-liquid separation: after the reaction is finished, separating the modified reducing agent from the enzymatic hydrolysate to obtain the solid modified reducing agent. Compared with the prior art, the method has the advantages that a biological enzyme catalysis process for modifying the reducing agent is provided, the energy consumption is low, the environment is protected, the cost is low, and the performance of the reducing agent can be remarkably improved.

The invention relates to the field of biological catalysis and enzyme engineering, and discloses a pH response type metal ligand-mutant metal dependent enzyme compound as well as a preparation method and application thereof. The compound is formed by compounding a mutant metal-dependent enzyme and a pH response type metal-ligand material, the mutant metal-dependent enzyme is obtained by directionally mutating amino acid residues in the metal-dependent enzyme, and the pH response type metal-ligand material is obtained by compounding the mutant metal-dependent enzyme and the pH response type metal-ligand material. The repulsive action of the amino acid residues on target metal ions in a metal ion transport channel of the metal-dependent enzyme is reduced or the amino acid residues and the target metal ions attract each other; the pH response metal-ligand material is formed by target metal ions and polydentate organic ligands through coordination, and the target metal ions can be complexed or released by adjusting pH. The efficiency that metal ions enter an enzyme activity center can be greatly improved, on-demand release and cyclic complexing of the metal ions are achieved, reutilization can be achieved through pH callback, the cost is effectively reduced, and excellent catalytic performance and environment friendliness are achieved.

The invention discloses a GSPT1 molecular glue degradation agent compound and application thereof, and particularly provides a compound shown as a formula I or pharmaceutically acceptable salt thereof. The invention aims to solve the technical problem that a high-selectivity and high-efficacy GSPT1 molecular glue degradation agent which can be used by GSPT1 dependent tumor patients is absent clinically at present, and provides a GSPT1 molecular glue degradation agent compound. The compound disclosed by the invention can be combined with an E3 ligase CRBN compound and change the specificity of the compound so as to induce ubiquitination and degradation of a compound related protein GSPT1, is novel in structure and has high selectivity and high degradation efficacy on the GSPT1 protein.

The invention discloses an engineering bacterium and a method for synthesizing 3 '-sialic acid lactose based on an RIAD-RIDD self-assembled multienzyme compound. The present invention provides a recombinant microorganism for the production of 3 '-sialic acid lactose wherein the recombinant microorganism has (a) enhanced enzymatic activity of CMP-N-acetylneuraminic acid synthetase and / or expression level of coding gene thereof compared to a wild-type microorganism or an original strain; (b) enhancing the enzymatic activity of the alpha-1, 3-fucosyltransferase and / or the expression level of the encoding gene of the alpha-1, 3-fucosyltransferase; and (c) the CMP-N-acetylneuraminic acid synthetase and the [alpha]-1, 3-fucosyltransferase enzyme form a complex. The recombinant microorganism provided by the invention can improve the intermediate channelization efficiency and yield, has improved substrate utilization rate and is strong in universality.

The invention relates to the field of natural product chemistry, sugar chemistry and biological medicine, and particularly provides glycosaminoglycan and / or a series of derivatives thereof as well as a preparation method and application of the glycosaminoglycan and / or the series of derivatives thereof, the glycosaminoglycan is extracted from starfish glabrata, and the preparation method comprises the following steps: extracting by adopting a papain combined alkaline hydrolysis method; the invention discloses an ALHX-2M series derivative and application of the ALHX-2M series derivative in prevention or treatment of thrombotic diseases, and the ALHX-2M series derivative is obtained by performing separation and purification through ion exchange column chromatography and molecular exclusion column chromatography to obtain ALHX-2M, and further performing deacetylation, deamination and depolymerization treatment and gel column chromatography separation and purification on the ALHX-2M series derivative and the application of the ALHX-2M series derivative in prevention or treatment of thrombotic diseases. The glycosaminoglycan and the derivative thereof take an endogenous blood coagulation factor X enzyme complex (FXase) as an action target, the anticoagulation activity and the antithrombotic activity of the glycosaminoglycan and the derivative thereof are superior to those of low-molecular-weight heparin, the bleeding risk under the equivalent antithrombotic dosage is remarkably reduced, and the glycosaminoglycan and the derivative thereof can be used for medicines or functional foods for preventing and treating thrombotic cardiovascular and cerebrovascular diseases.

This disclosure relates to mRNA therapy for the treatment of maple syrup urine disease (MSUD). mRNAs for use in the invention, when administered in vivo, encode branched chain α-ketoacid dehydrogenase complex (BCKDC) E1α, E1β, or E2mRNA therapies of the disclosure increase and / or restore deficient levels of E1α, E1β, or E2 expression and / or BCKDC activity in subjects. mRNA therapies of the invention further decrease abnormal accumulation of branched chain amino acids associated with deficient BCKDC activity in subjects.

The invention discloses a kit for catalyzing Friedel-Crafts acylation reaction through an enzyme method, application and a preparation method. The kit for catalyzing the Friedel-Crafts acylation reaction by the enzyme method comprises 1) a Friedel-Crafts acylation reaction buffer solution and 2) a thiolase complex MucABC or a functional fragment and a variant thereof. The kit for catalyzing the Friedel-Crafts acylation reaction does not depend on coenzyme A compounds as acyl donors, and can realize regional specific C-acylation modification on various lactam compounds by taking acyl phenyl esters with different chain lengths as the acyl donors under mild conditions; a novel green and efficient biological catalysis tool is provided for Friedel-Crafts acylation reaction, and the method has important value and significance for drug synthesis and chemical production.

The invention provides an application of an inhibitor of a targeted FIGNL1-FIRRM-MACIR complex in preparation of a tumor drug and a composition of the inhibitor of the targeted FIGNL1-FIRRM-MACIR complex. According to the research, the MACIR is identified as a bridge-like adaptive subunit of a FIGNL1-FIRRMAAA + ATP (Adenosine Triphosphate) enzyme complex. The MACIR is directly combined with the FIRRM and the DNA to promote the FIGNL1 mediated RAD51 filamentous structure to be unfolded, so that the damage repair of cross-linking (ICLs) between DNA chains induced by aldehydes or platinum drugs is promoted, and the genome stability is maintained. Pharmacological inhibition is carried out on the FIGNL1-FIRRM-MACIR complex by using two inhibitors, namely, 4-{2-[(5-chloro-2-methoxyanilino) carbonyl] anilino}-4-oxobutyric acid and 2-{[5-(2-ethoxyethyl)-4-hydroxy-6-oxo-1, 6-dihydro-2-pyrimidinyl] sulfenyl}-N-(3-methoxyphenyl) acetamide, which are used for the FIGNL1-FIRRM-MACIR complex, so that the FIGNL1-FIRRM-MACIR complex can be prepared by using the two inhibitors. The cytotoxic effect of platinum drugs in ovarian cancer can be obviously enhanced.

A general-purpose selenium-enriched pearl-based basic skincare mother liquor, by weight, is composed of pearl active peptides, selenium yeast extract, and an enzyme complex in a weight ratio of 1:(0.00625~0.1):(0.0375~0.25). The pearl active peptides have a molecular weight <1kDa, an amino acid content ≥90mg / mL, and are colorless and transparent. The selenium yeast extract has a particle size <80nm, selenocysteine ​​≥60%, and a Zeta potential of +18 to +23mV, and is a colorless and transparent dispersion. The enzyme complex contains SOD ≥2000U / mg and CAT ≥1500U / mg, and is encapsulated in a chitosan-sodium alginate bilayer, making it colorless and semi-transparent. This basic skincare mother liquor addresses the problems of low dissolution and delivery rates of pearl's effective components, poor stability of selenium components, and lack of multi-effect synergistic mechanisms. It can be used to rapidly prepare various types of cosmetics, such as serums (for external or oral use) and masks.

The invention belongs to the field of synthetic biology, and discloses an artificial carbon dioxide fixation method and application thereof. According to the method, an artificial carbon dioxide immobilization metabolic pathway is constructed, the pathway is a linear autocatalytic cycle pathway LATCH, acetyl coenzyme A and derivative products thereof can be synthesized by using carbon dioxide or bicarbonate, the catalytic cycle pathway takes two molecules of CO2 or HCO3 <-> as a substrate, one molecule of acetyl coenzyme A is generated through ten enzymatic steps, operation of the pathway depends on ATP and NADPH, and the acetyl coenzyme A can be synthesized by using the linear autocatalytic cycle pathway LATCH. Or is driven by an energy and reducing power supply system, such as organic matters such as glucose, formic acid and the like, high-energy compounds such as polyphosphoric acid, phosphocreatine and the like, or a photoreaction enzyme complex on a plant capsule membrane. An in-vitro multi-enzyme system and recombinant escherichia coli experiment prove that the acetyl coenzyme A and the derivative polyhydroxybutyric acid thereof can be efficiently synthesized, and the application prospect in the fields of carbon neutralization, green manufacturing and the like is wide.

The invention discloses an expanded artificial scaffold protein, an artificial fibrosome and a preparation method and application thereof, and belongs to the technical field of enzyme engineering. According to the invention, the scaffold proteins containing different numbers of adhesion modules are designed, and the two scaffold proteins are modularly assembled by using a covalent linkage system, so that the oversized artificial scaffold protein of which the number of adhesion modules can reach 19 is successfully constructed, and the scale of the oversized artificial scaffold protein exceeds that of the known natural scaffold protein. The scaffold protein can efficiently carry various catalytic subunits through specific interaction of an adhesion module and a docking module to form an artificial fibrosome. Experiments show that the artificial cellulosome can efficiently degrade cellulose substrates such as crystalline cellulose and the like especially when the scaffold protein contains an extremely high amount of cohesin. The invention provides a novel efficient enzyme complex tool for efficiently degrading lignocellulose biomass, and has a wide application prospect in the fields of biofuel, bio-based chemical production and the like.

The application relates to an ordered sensing interface based on a DNA-enzyme composite nanostructure and a construction method and application thereof. The ordered sensing interface comprises an electrode and a DNA-enzyme composite nanostructure connected to the electrode; the DNA-enzyme composite nanostructure comprises a DNA electronic transmission module and an enzyme catalysis module; the DNA electronic transmission module comprises a mediator-containing DNA sheet structure; the enzyme catalysis module comprises an enzyme substrate complex connected to the DNA sheet structure; and the enzyme substrate complex, the mediator-containing DNA sheet structure and the electrode are sequentially and orderly distributed in space in the order of enzyme substrate complex-mediator-containing DNA sheet structure-electrode. Through the design of the enzyme substrate complex and the DNA sheet structure, the enzyme is orderly fixed, the influence of spatial interference and non-specific interaction on electron transmission is reduced, and the embedded mediator can improve the electrode interface electron transmission rate.

Provided is a composition for performing gene editing on cells. The composition comprises: a nickase complex or an encoding nucleic acid molecule thereof, wherein the nickase complex comprises a nickase, an optional guide nucleic acid molecule, and a first recruitment component; a target nucleic acid molecule containing a target site homologous sequence, wherein the target nucleic acid molecule can be linked to or bind to a second recruitment component, the first recruitment component and the second recruitment component are suitable for forming specific binding, the nickase complex is suitable for generating a nick in a single strand of DNA in a genome without cleaving the double strands of the DNA, and the nickase complex is configured to be linked to the first recruitment component; and a target nucleic acid molecule containing homologous arms at both ends and a repair template, wherein the target nucleic acid molecule is configured to be linked to or bind to the second recruitment component, and the first recruitment component and the second recruitment component are suitable for forming specific binding.

The invention discloses a preparation method of a kit for detecting human papilloma, and particularly relates to the technical field of gene fragment detection.The kit comprises a micro-fluidic chip, independent reaction units are arranged in the chip in a partitioned mode, and each unit comprises a primer group for specific subtypes of HPV, isothermal amplification enzyme, a CRISPR-Cas12a compound and a quantum dot fluorescent probe; the freeze-drying reagent comprises the primer group wrapped by a freeze-drying protective agent, an isothermal amplification enzyme, a CRISPR-Cas12a (clustered regularly interspaced short palindromic repeats-associated 12a) compound and a quantum dot fluorescent probe. Through cooperation of microfluidic integration, isothermal amplification, CRISPR verification and quantum dot labeling, the bottleneck of traditional HPV detection in sensitivity, specificity and convenience is broken through, meanwhile, the clinical diagnosis value is improved through the double-target design, and the application scene is expanded through the freeze-drying process.

The application discloses a rice large-scale metabolic regulation sub-DGP1 and crop metabolic quality design. The application finds that a rice DGP1 family protein TIGR01589 domain targets multiple metabolic enzymes and promotes the transport of the metabolic enzymes to the nucleus; the domain also interferes with the light respiration pathway by changing the composition of the glycine decarboxylase complex in mitochondria. Based on the molecular mechanism of DGP1, a chimeric protein ST containing the domain and coupled with a chloroplast localization signal is designed to realize the genetic engineering modification of Chinese cabbage. The modification strategy can not only guide the substrate flow of the central glycolytic pathway to flavonoid biosynthesis, but also promote the accumulation of photosynthetic product-derived sugars in leaves.

The invention relates to a method for labeling antigen protein by biotin. The method comprises the following steps: (1) providing a ligase compound formed by Ni < 2 + > chelated polypeptide and recombinant Turbo ID protein; (2) providing a recombinant antigen protein with HisTag (HisTag); and (3) the recombinant antigen protein with HisTag reacts with biotin in the presence of the ligase complex, ATP and Mg < 2 + > to obtain the biotin-labeled antigen protein, in the step (1), the amino acid sequence of the polypeptide is shown as SEQ ID NO: 1, and the amino acid sequence of the recombinant Turbo ID protein is shown as SEQ ID NO: 2. According to the method disclosed by the invention, the labeling efficiency when the antigen protein is labeled by biotin is improved, the biotin utilization rate is improved, and the positive detection rate of specific detection of the antibody in a clinical sample is improved.

This invention relates to the field of aspartic acid technology, specifically to an aspartic acid raw material and its preparation method. The method includes loading a substrate solution and an enzyme complex into an immobilized reactor for reaction, collecting the reaction products, crystallizing the reaction products to precipitate aspartic acid crystals, and then refining the aspartic acid crystals to form purified aspartic acid. The nano-titanium tube mineralization treatment in this invention significantly improves the stability of the enzyme complex, while the dual modification with silane and dopamine enhances the dispersibility and biocompatibility of ammonium fumarate, promoting enzyme-catalyzed reaction efficiency. Simultaneously, the integrated crystallization-refining process, through pH control and gradient cooling, achieves efficient precipitation and purification of aspartic acid crystals. The entire preparation process is green and environmentally friendly, with filtrate recycling reducing wastewater discharge, meeting the sustainable development needs of the modern pharmaceutical industry and demonstrating significant economic and environmental benefits.

The invention discloses a gel composition for repairing female genital tracts as well as a preparation process and application of the gel composition. The composition comprises three functional core systems: a polysaccharide-enzyme compound A which is formed by self-assembly of superoxide dismutase and oxidative degradation modified aloe polysaccharide and has antioxidant and microenvironment regulation functions; a metal ion-polysaccharide slow-release antibacterial body B formed by coordination of zinc ions and low-molecular-weight chitosan provides instant and long-acting antibacterial protection; the plant polyphenol-protein tissue repair body C is formed by non-covalent binding of epigallocatechin gallate and hydrolyzed collagen, and tissue regeneration and functional reconstruction are promoted. The three components are compounded according to a mass ratio of (1-2): (0.5-1): (2-4), and are integrated with a carbomer matrix to form stable gel. The invention further provides a specific preparation process and a synergistic effect mechanism of all the components. The gel has good stability, biocompatibility and repairing efficiency, and is suitable for treating or improving female genital tract mucosa lesion, inflammation or flora imbalance.

The application belongs to the technical field of food engineering, and particularly relates to a food-grade emulsion enzyme catalysis system, a construction method and application in functional lipid synthesis. The food-grade Pickering emulsion is formed by taking water-insoluble protein-chitin nanoparticles and a lipase CRL compound as the water phase and taking a reaction substrate fatty acid as the oil phase, and is used for preparing phytosterol esters. The water-insoluble protein-chitin nanoparticles have excellent dispersibility, stability and high surface positive charge, the lipase is adsorbed on the surface of the nanoparticles through electrostatic and hydrogen bond interaction, has reduced interfacial tension and enhanced interfacial activity, the lipase in the Pickering emulsion stabilized by the nanoparticles is distributed on the oil-water interface, the catalytic efficiency of the lipase is effectively improved, and the food-grade Pickering emulsion interface catalysis is used to efficiently synthesize phytosterol esters.