185 results about "Nanopore sequencing" patented technology

The present invention provides systems, methods, and compositions for performing molecular tests. In particular, the present invention provides methods, compositions and systems for generating target sequence-linked solid supports (e.g., beads) using a solid support linked to a plurality of capture sequences and capture primers composed of a 3′ target-specific portion and a 5′ capture sequence portion. In certain embodiments, the target sequence linked solid support is used in sequencing methods (e.g., pyrosequencing, zero-mode waveguide type sequencing, nanopore sequencing, etc.) to determine the sequence of the target sequence (e.g., in order to detect the identity of a target nucleic acid in sample).

The invention relates to a method for modifying a template polynucleotide for characterization, especially for nanopore sequencing. The method produces a modified polynucleotide which is complementary to the template polynucleotide at some positions and which contains universal or abasic nucleotides at the other, and in some instances predicable, positions. The resulting modified polynucleotide can then be characterized.

The invention discloses a method for carrying out deceleration and monomolecular capture on a nucleic acid molecule based on a solid-state nano hole. The method comprises the following steps of: adding the nucleic acid molecule to be measured into a nano hole sequencing device filled with an electrolyte, wherein the nano hole sequencing device comprises an electrolytic bath provided with an anode and a cathode, and a solid-state nano hole thin film for separating the anode of the electrolytic bath from the cathode of the electrolytic bath; placing the nucleic acid molecule in a positive chamber of the electrolytic bath; during measurement, introducing a pressure external field into the positive chamber as a driving external field for the perforation of the nucleic acid molecule; applying a voltage between the anode and the cathode as an electric field external field reverse to the pressure external field; and in a measurement process, enabling the acting force of the pressure external field, from which the nucleic acid molecule is suffered in the nano hole to be more than or approximately equal to electric field force according to a deceleration purpose or a capture purpose to be realized by the method. By using the method, on the premise that the signal-to-noise ratio of a measurement signal is not decreased, the speed at which a DNA (deoxyribonucleic acid) molecule penetrates through a nano hole device can be effectively decreased, and a perforation speed can be slowed down for above one order of magnitude.

The invention relates to a method for modifying a template double stranded polynucleotide, especially for characterisation using nanopore sequencing. The method produces from the template a plurality of modified double stranded polynucleotides. These modified polynucleotides can then be characterised.

The invention relates to devices and methods for nanopore sequencing. The invention provides for using the signals from n-mers to provide sequence information, for example where the system has less than single base resolution. The invention includes arrays of nanopores having incorporated electronic circuits, for example, in CMOS. In some cases, the arrays of nanopores comprise resistive openings for isolating the electronic signals for improved sequencing. Methods for controlling translocation of through the nanopore are disclosed.

The invention relates to a method for modifying a template double stranded polynucleotide, especially for characterization using nanopore sequencing. The method produces from the template a plurality of modified double stranded polynucleotides. These modified polynucleotides can then be characterized.