104 results about "Legionella" patented technology

The present invention provides ketolide derivatives, which can be used as antibacterial agents. In particular, compounds described herein can be used for treating or preventing conditions caused by or contributed to by Gram-positive, Gram-negative or anaerobic bacteria, more particularly against, for example, Staphylococci, Streptococci, Enterococci, Haemophilus, Moraxalla spp. Chlamydia spp., Mycoplasm, Legionella spp., Mycobacterium, Helicobacter, Clostridium, Bacteroides, Corynebaclerium, Bacillus or Enterobactericeae. Also provided are processes for preparing such ketolide derivatives, pharmaceutical compositions thereof, and methods of treating bacterial infections.

The invention provides a kit for detecting 10 respiratory tract infection pathogens and an application method thereof. The kit comprises primers and a TaqMan probe, wherein the primers are used for amplifying the 10 respiratory tract infection pathogens which include mycoplasma pneumoniae, chlamydia pneumoniae, legionella pneumophila, bordetella pertussis, rhinovirus, respiratory tract adenovirus, influenza A virus, influenza B virus, respiratory syncytial virus and parainfluenza virus. The application method includes: mixing 2 microliters of sample DNA / RNA co-extraction template, 20 microliters of RT-PCR buffer, 1 microliter of mixed enzyme liquid and 2 microliter of primer-probe mixed liquid to perform PCR amplification reaction. The kit uses the Taqman probe fluorescent PCR technology to detect the 10 common respiratory tract infection pathogens, a detection result can be obtained within 2 hours, the kit is high in specificity, and the sensitivity of the kit can reach 100copies / microliter.

An efficient composite biocide for killing the bacteria, fungi, algae, legionella, etc in circulated cooling water system is proportionally prepared from glutaric dialdehyde, quaternary ammonium salt, assistant, etc.

The present invention provides Gyrase B and / or Topoisomerase IV par E inhibitors, which can be used as antibacterial agents. Compounds disclosed herein can be used for treating or preventing conditions caused by or contributed by gram positive, gram negative and anaerobic bacteria, more particularly against, for example, Staphylococci, Streptococci, Enterococci, Haemophilus, Pseudomonas spp., Acenetobacter spp., Moraxalla spp., Chlamydia spp., Mycoplasma spp., Legionella spp., Ktycobacterium spp., Helicobacter, Clostridium spp., Bacteroides spp., Corynebacterium, Bacillus spp., Enterobactericeae,’ (E. coli, Klebsiella spp., Proteus spp., etc.) or any combination thereof. Also provided, are processes for preparing compounds disclosed herein, pharmaceutical compositions containing compounds disclosed herein, and methods of treating bacterial infections. (Formula)

The present invention provides ketolide derivatives, which can be used as anti-bacterial agents. Compounds disclosed herein can be used for the treating or preventing conditions caused by or contributed to by gram positive, gram negative or anaerobic bacteria, more particularly against, for example, Staphylococci, Streptococci, Enterococci, Haemophilus, Moraxalla spp., Chlamydia spp., Mycoplasm, Legionella spp., Mycobacterium, Helicobacter, Clostridium, Bacteroides, Corynebacterium, Bacillus, Enterobactericeae or any combination thereof. Also provided are processes for preparing compounds disclosed herein, intermediates used in their synthesis, pharmaceutical compositions thereof, and methods of treating bacterial infections.

The invention relates to a multiplex PCR-based synchronous and rapid method for detecting 13 pathogenic microorganisms in water, which comprises the steps of using multiplex PCR to simultaneously amplify gene-specific fragments of the 13 pathogenic microorganisms including escherichia coli, enterohaemorragic escherichia coli o157:h7, legionella pneumophila, salmonella enteritidis, shigella dysenteriae, staphyloccocus aureus, listeria monoeytogenes, helicobacter pylori, mycobacterium tuberculosis, klebsiella pneumonia, vibrio cholera, bacillus anthracis and yersinia pestis, and detecting PCR amplified products through agarose gel electrophoresis, thereby achieving synchronous and rapid detection for the 13 pathogenic microorganisms.

Provided herein are ketolide derivatives, which can be used as antibacterial agents. Compounds described herein can be used for treating or preventing conditions caused by or contributed to by gram positive, gram negative or anaerobic bacteria, more particularly against, for example, Staphylococci, Streptococci, Enterococci, Haemophilus, Moraxalla spp., Chlamydia spp., Mycoplasm, Legionella spp., Mycobacterium, Helicobacter, Clostridium, Bacteroides, Corynebacterium, Bacillus, Enterobactericeae, Propionibacterium acnes or any combination thereof. Also provided are processes for preparation of compounds described herein, pharmaceutical compositions thereof, and methods of treating bacterial infections.

The invention discloses a novel sputasol for isolated culture of Legionnella, which can be used for sputamentum digestion in isolated culture of Legionnella and can be also used as a digestive juice for conventional bacterial culture of clinical sputamentum. The sputasol comprises the following components: 2.0g of dithiothreitol (DTT), 0.894g of ethylene diamine tetraacetic acid (EDTA), 0.4g of potassium chloride, 3.6g of dipotassium hydrogen phosphate, 0.4g of potassium dihydrogen phosphate and 100g of water. By using the DTT, the sputasol breaks disulfide bonds in mucoproteins, thereby degrading the mucoproteins and digesting the sputamentum. By adding the EDTA into the sputasol, the EDTA can react with alkali metal, rare earth elements, transition metal and the like to form stable water-soluble complexes, thereby avoiding the poison of heavy metallic salts on the Legionnella. A buffer system comprises KCl, K2HPO4, KH2PO4 and other potassium salts and avoids the inhibiting effect of Na<+> ions on the Legionnella, thereby improving the positive rate in isolated culture of the Legionnella.

Provided herein are substituted aromatic compounds, which are tRNA synthetase inhibitors, and hence can be used as antimicrobial agents. Compounds described herein can be used for the treatment or prevention of a condition caused by or contributed to by gram positive, gram negative, anaerobic bacteria or fungal organisms, more particularly against bacterium, for example, Staphylococci, Enterococci, Streptococci, Haemophilus, Moraxalla, Escherichia, Chlamydia, Mycoplasm, Legionella, Mycobacterium, Helicobacter, Clostridium, Bacteroides, Corynebacterium, Bacillus or Enterobactericeae, and fungal organisms, for example, Aspergillus, Blastomyces, Candida, Coccidiodes, Cryptococcus, Epidermophyton, Hendersonula, Histoplasma, Microsporum, Paecilomyces, Paracoccidiodes, Pneumocystis, Trichophyton, or Trichosporium. Processes for the preparation of these compounds, pharmaceutical compositions thereof, and methods of treating microbial infections are also provided.

A method of controlling Legionella bacteria in cooling water systems is described. The method involves the heating of recirculating cooling water to temperatures of 70° C. for sufficient time to kill the bacteria. The method functions best in combination with chemical bactericides.

The invention discloses a primer group for detecting respiratory tract microorganisms based on a nanopore sequencing method. The nucleotide sequences of the primer group are as shown in SEQ ID NO:1-20. The microorganisms are streptococcus pneumoniae, staphylococcus aureus (resisting to methicillin), klebsiella pneumoniae, pseudomonas aeruginosa, acinetobacter baumannii, stenotrophomonas maltophilia, candida albicans, haemophilus influenzae, legionella pneumophila, enterococcus faecium, chlamydia psittaci, cryptococcus gattii, aspergillus fumigatus and pneumocystis jiroveci. According to the invention, sequencing optimization is carried out on different types of samples of the respiratory tract microorganisms, so that the detection method, the kit and the like are suitable for various typesof respiratory tract samples; and a targeted amplification method is adopted, so that the detection requirements of sputum, alveolar lavage and other sample types can be met. In addition, parallel sequencing can be performed, so that the flux of the detected samples is increased, the sequencing time is shortened, and the contradiction between the flux of detected pathogenic species and the cost and time is further relieved.

A growth inhibitor of bacteria of the genus Legionella contains as an active ingredient a Macaranga tanarius extract extracted from Macaranga tanarius with an extraction solvent including at least an organic solvent. Alternatively, the growth inhibitor of bacteria of the genus Legionella contains as an active ingredient at least one selected from nymphaeol-A, nymphaeol-B, and nymphaeol-C. The growth inhibitor of bacteria of the genus Legionella is used by being blended to, for example, a bath agent or a cleaning agent.

A water hygiene improving system and method are provided which may include and utilize one or more water hygiene improving devices which may be coupled to a building water supply network at any number of locations so that water passing through each water hygiene improving device is returned to the building water supply network. The water hygiene improving device may include one or more membrane filtration systems and optionally one or more bioreactors. The system and method include a new and innovative approach for surviving or improving water hygiene within the whole water supply network of new or existing buildings, for prevention of their contamination with Legionella or other pathogens, general limitation of biofilm growth and their negative effects on water hygiene, also at point of use (POU), and water distribution within new or existing buildings by limitation of incoming nutrients, bacteria and other microorganisms at point of entry (POE) of buildings or at other locations of the water supply network within the building.

An electrolytic assembly and a method for the bacterial disinfection of water or wastewater is disclosed. Water circulating in cooling towers such as those that discharge heat from air conditioning; ships' ballast water; or wastewater with a dryness varying from 0.01 to 3%; can be treated. The assembly comprises one or more electrolytic units comprising at least one Dimensionnally Stable Anode commonly known as DSA, or a Boron Doped Diamond anode, also named BDD anode. The electrolytic treatment at least partially kill the bacteria present in the water. It has been shown that the electrolytic treatment breaks the cell membrane of bacteria present in the water. The treatment is particularly adapted for eliminating Legionella and others microorganisms, such as E. coli.

The invention provides a primer composition for detecting legionella in clinical and environmental specimens. The primer composition comprises a pair of primers (shown in the Seq ID No.1-2) for PCR specific amplification of a gene mip and a pair of primers (shown in the Seq ID No.3-4) for PCR specific amplification of 16S rRNA. The invention also provides a detection kit containing the primer composition. A test on 320 groups of phlegm and alveolar irrigation solutions of pneumonia patients and 210 groups of environmental water specimens proves that the primer composition has singularity of 100% and a lowest detection limit of 2*10<1> cfu / ml. The dual PCR system can be successfully used in bacterial strain identification and clinical and environmental specimen detection. The PCR detection method built based on the dual PCR system has the characteristics of simpleness, fastness and reliability and can be widely used in epidemiology preliminary investigation, environmental water monitoring and clinical specimen detection.

A method for providing biological control to a flow of water in a recirculating potable hot water system. In such a system, water is subjected to an initial chemical treatment using an oxidizing biocide such as chlorine dioxide or peracetic acid that rapidly kills biofilm and Legionella bacteria. This treatment is continued until existing Legionella bacteria and biofilm are controlled. Once the bacteria and biofilm are controlled the chemical treatment is stopped. Starting concurrently with the chemical treatment or at the termination of the chemical treatment, non-chemical water treatment is provided. The non-chemical water treatment maintains the water system in a condition which is free of Legionella bacteria and biofilm. Chemical water treatment may be repeated intermittently should it prove desirable or necessary.

The invention discloses a gene chip-based method for synchronously and rapidly detecting thirteen pathogenic microorganisms in a water body. The method is characterized in that specific gene segments of the pathogenic microorganisms are amplified simultaneously by adopting multiple polymerase chain reactions (PCR), and then, a detection result is obtained through gene chip hybridization and chip scanning image analysis. The detected thirteen pathogenic microorganisms comprise escherichia coli, Enterohemorrhagic Escherichia coli O157:H7, legionella pneumophila, salmonella enteritidis, Shigella, staphylococcus aureus, Listeria monocytogenes, Helicobacter Pylori, mycobacterium tuberculosis, Klebsiella pneumonia, vibrio cholera, Bacillus anthracis and Yersinia pestis.

An antibacterial agent in the form of crystal for killing the Legionella in the air channel of centralized air conditioner, pneumobacillus, typhoid bacillus, staphylococcus aureus, and more than 30 moulds is prepared from Paris rhizome, garlic oil and pulsatilla root.

A method of controlling protozoa trophozoites and cysts is disclosed. The method comprises the addition of guanidine or biguanidine salts to aqueous systems harboring protozoa trophozoites and cysts. Addition of such materials to aqueous systems was found to be effective at controlling both the protozoa trophozoites and cysts. By effectively controlling both the protozoa trophozoites and cysts, organism such as Legionella cells harbored in both the trophozoites and cysts are in turn controlled.

The invention provides a superbacteria antibiotic disinfectant, a preparation method and a purpose thereof. The antibiotic disinfectant comprises the following components by content: 1-100 g / L of a composite ion antibacterial material, 10-15 g / L of nano ZnO-modified dihydroxy diphenyl silane, 1-100 g / L of a film forming agent, 1-100 g / L of a thickening agent, 1-10 g / L of a plant extraction solvent, 1-10 g / L of a solubiliser, 1-10 g / L of a refrigerant, and the balance of water. The superbacteria antibiotic disinfectant can be used for killing methicillin-resistant staphylococcus aureus (super bacterium), escherichia coli, legionella bacillus, pyogenic coccus, hepatitis virus, candida albicans and staphylococcus aureus in various epidemic infectious diseases and bacterial spores, and is used for article surface and environment disinfection in high-speed rail, airplane, steamship, subway, hospital, school, kindergarten, large-scale stadium, large-scale entertainment facility and other public places as well as used for epidemic area disinfection.

The invention provides a reagent for legionella pneumophila detection according to an in-vitro nested loop-mediated isothermal amplification method. The reagent comprises an outer primer F3, an outer primer B3, an inner primer FIP, an inner primer BIP, a loop primer Loop-f, a loop primer Loop-r, a nested primer FNP and a nested primer BNP. The invention further provides a novel efficient in-vitro isothermal amplification based legionella pneumophila detection method adopting the reagent. Quickness and accuracy in legionella pneumophila detection are realized by adoption of the method, sample detection can be finished in 30-45 minutes approximately, LAMP (loop-mediated isothermal amplification) reaction time is shortened by about 30%-50% as compared with that of a method with loop primers added, and great practical significance to quick disease diagnosis is achieved.

The invention provides an indoor air conditioner for purifying polluted air and eliminating legionella. The whole indoor air conditioner is cylindrical and formed by sequentially sleeving an upper air purifying section, an intermediate draught fan section and a lower air conditioner cooling and heating coil section. The indoor air conditioner can efficiently and quickly purify PM2.5, and eliminate legionella, virus, influenza virus, germs, mould and acarid. The indoor air conditioner is characterized in that a unique layering stacking form is adopted; a unique ventilating channel is arranged; a unique purifying section enables direct viewing of pollutants; unique stacked trays are used for storing nanogel which is used as a purifying agent; no ice is generated in refrigerating, and the air volume of an air outlet is stable. Therefore, the indoor air conditioner is superior to traditional packaged air conditioners, hanging air conditioners, automobile air conditioners and central air conditioners. The indoor air conditioner has the advantages that the quantity of the pollutant PM2.5 can be directly viewed at any time while the indoor polluted air enters the purifying section, and the stacked trays can be replaced at any time according to the pollution level, so that the indoor air cleanliness can be effectively protected, and the circulating air is free of PM2.5; refrigerating and heating pipes and blades of the draught fan are free of dirty gel, and pollutants are not attached to the refrigerating and heating pipes and the blades of the draught fan; the size of the air conditioner is small; the air volume is large; the noise is low; a motor is long in service life; more electricity can be saved; the refrigerating and heating are quick; the cost is low; the pollutant absorbing and purifying capacity is high; the stacked trays can be flexibly increased and decreased according to indoor pollution level; the air conditioner has a wide market prospect.

A method of controlling Legionella bacteria in cooling water systems is described. The method involves the heating of recirculating cooling water to temperatures of 70° C. for sufficient time to kill the bacteria. The method functions best in combination with chemical bactericides.

The invention relates to an early warning method for Legionella, belonging to an automatic detecting method. The system of the invention comprises an early warning centre which is connected with multiple detecting subsystems mounted on the detection field through a public communication network, wherein each detecting subsystem comprises a signal transmitting and receiving device connected with the public communication network and connected with a control and signal processing device; and the control and signal processing device is connected with a physical and chemical detecting subsystem which is mounted on a monitored water source and used for detecting the survival environment of the Legionella, and a biochemical detecting subsystem which can be used for extracting a field water sample from the monitored water source and detecting the water sample according to colloidal gold. By using the early warning centre provided by the invention, hundreds of or even thousands of detecting subsystems can be detected on schedule through the public communication network, thereby realizing early warning of the Legionella in a large city and largely saving manpower and material resources. By means of the early warning system and method disclosed by the invention, automatic and normalized Legionella detection can be realized and the outbreaks of epidemic diseases caused by the Legionella can be effectively prevented.

The invention discloses a method and special kit for authenticating the legionella toxicity. The method comprises the following steps: (1) setting up parameters: Spectral acquisition setup; Grating scan type: Extended; Confocality: Standard; Spectrum Range: Low 100.00; Centre Raman shit / cm<-1>; High 2000.00; Configuration: Laser name 532nm edge; Grating name 18001 / mm; Exposure time / s: 10.00; Accumulations: 1; Objective: 50; Laser power%: 100; (2) detecting; and (3) acquiring a Roman spectrogram. Within an x-coordinate range of 500-2000, if more than one peak with the intensity of 4000 above exists, the to-be-detected legionella is a legionella virulent strain; if the conditions above are not met, the to-be-detected legionella is a legionella attenuated strain. The method disclosed by the invention is rapid, simple and convenient and is low in detection cost.

A method is disclosed, which is capable of subjecting microorganisms to sterilizing or bacteriostatic treatment with good efficiency as compared with the conventional sterilizing or bacteriostatic method using a noble metal electrode. Microorganism-containing water to be treated is electrochemically treated using an anode having conductive diamond to bring the microorganism into contact with the anode, thereby undergoing sterilization. Since the conductive diamond has a high oxidation potential as compared with other electrode substances, direct oxidation reaction due to contact between a microorganism in water to be treated, such as Legionella bacteria, and the anode surface occurs strongly as compared with other electrodes, thereby enabling effective sterilization. The conductive diamond has a high ability to generate ozone and has an excellent formation efficiency with respect to the generation of hydrogen peroxide and radicals. Accordingly, an indirect sterilizing effect can also be expected.

A device and method for qualitatively and / or quantitatively detecting Legionella in water or aqueous solutions comprising at least one inlet for introducing a sample volume into a filtration cell, a filtration device, a device for separating immunomagnetically bonded Legionella, a detection cell, a device for transferring immunomagnetically bonded Legionella into the detection cell, a device for exciting a fluorescence of Legionella having fluorescent marking, and a detector unit for detecting the fluorescence of Legionella having fluorescent marking is provided. The microorganisms present in a sample volume can be filtered out of the sample volume by means of the filtration device in the filtration cell, immunomagnetic compounds for selectively marking Legionella can be introduced into the retentate of the filtration cell, and the immunomagnetically bonded Legionella can be separated from other microorganisms by means of the device and transferred into the detection cell by means of a transfer device.

The invention provides a bactericidal cleaner. The bactericidal cleaner is prepared by mixing the following raw materials in parts by weight: 2-8 parts of sodium benzene sulfonate, 1-4 parts of trifluoroethanol, 1-2 parts of sodium hydroxide, 1-10 parts of sodium chloride, 5-15 parts of ethanol, 5-10 parts of lightyellow sophora root, 8-10 parts of camphor, 4-10 parts of 1,2-dichloroethylene, 10-20 parts of mixture of trichloroethylene and tetrachloroethylene and 10-20 parts of alcohol ether. The bactericidal cleaner has the following advantages and positive effects that 1. the finished product does not contain phosphorus or organic solvents, and the residual liquid after cleaning can not pollute the ecological environment; 2. the bactericidal cleaner has strong detergency and good cleaning effect and has bactericidal effects on acarids, legionella and the like; and 3. the bactericidal cleaner has good wettability and can elute various oil from various adsorbents easily.

The invention relates to a filtration system for microorganisms such as legionella in an air duct of a central air conditioner and a filtering method thereof, and belongs to the field of air conditioning and purification. The filtration system is arranged at an entrance of a central air conditioner cabinet, a primary filtration device, a secondary filtration device and air dewater device and a cooling device are used. Hot water using solar energy and hot water flowing into a cooling tower as heat sources is used as a primary filtration device of the legionella, lithium chloride is used as a secondary filtration device of the legionella and dewater device of air, and cooling water flowing out of the cooling tower is used as a cooling device of air. The filtration system for the microorganisms such as the legionella in the air duct of the central air conditioner is high in integration level and good in filtering effect, and can effectively prevent the diffusion of bacteria and the microorganisms in a central air-conditioning system and can prevent the cooling and cracking of a coil pipe in the air-conditioning cabinet in winter.