9 results about "Legionella" patented technology

The invention discloses a secondary water supply quality remote monitoring system, belongs to the technical field of water quality detection, and solves the problem of legionella outbreak caused by hydraulic stagnation and high-temperature environment coupling of a secondary water supply system. However, the problems of detection space randomness, sampling false negative and high-cost monitoring blind area due to random distribution of the pathogenic bacteria in an isolated pipe section are solved. Comprising a main control and calculation module, an electrochemical impedance spectroscopy measurement module, a micro-current measurement module, a multi-channel switching module, a temperature sensing module, a bus power supply and communication module and a three-electrode sensing patch, the master control and calculation module is used for coordinating and controlling the operation time sequence of all the modules, executing an electrochemical impedance spectrum fitting and micro-current trend analysis algorithm and making a risk decision according to a multi-source data fusion result. The water quality at the tail end of each pipeline is automatically and circularly monitored, the biofilm formation risk is evaluated in real time by measuring the temperature, executing electrochemical impedance scanning, analyzing weak metabolic current and fusing data, and finally early warning information is uploaded.

The application discloses a digital image quantitative dual-mode detection system and method based on a cascade enhanced nano-enzyme, which comprises a probe prepared from a membrane nano-enzyme material, an immunochromatography test strip and a signal automatic analysis system, the nano-enzyme material takes molybdenum disulfide nanosheets as a substrate, sequentially loads large-diameter platinum-iridium alloy particles and small-diameter platinum-iridium alloy particles through polyethylene imine-mediated electrostatic self-assembly, and forms a high-density catalytic active site array. The probe is coupled with specific antibodies on the surface of the nano-enzyme material. Based on the inherent light absorption and peroxidase-like activity of the probe, a dual-mode detection strategy of physical color development-catalytic enhancement is established, high-concentration sample visual preliminary screening and low-concentration sample ultra-sensitive quantitative detection are realized, and combined with an adaptive cross-peak colorimetric quantitative algorithm, individual visual perception differences can be effectively eliminated, and on-site rapid quantitative screening of community-acquired pneumonia pathogens such as streptococcus pneumoniae and legionella pneumophila can be realized.

The application provides a quality control for detecting respiratory pathogen nucleic acid, which comprises any one or more of the following respiratory pathogens: coronavirus, influenza virus, adenovirus, Mycoplasma pneumoniae, Streptococcus pneumoniae, rhinovirus or / and Legionella pneumophila. The full-length genome sequence of the coronavirus is divided into 6 target fragments, each with a length of 4000-5500 bp. The quality control of the application covers all detection target sequences (or target points) of the coronavirus, has a wide coverage, comprehensively detects target points, and does not have the phenomenon of missed detection. The quality control of the application contains main respiratory pathogen infections, has a wide range, and uses real virus samples and lentivirus samples as raw materials, and has more accurate detection target points. The preparation of the coronavirus quality control of the application integrates the target gene sequence into the host genome through the lentivirus vector, knocks out the self-replication gene in the preparation process, and has a "self-inactivation" ability, so that the recombinant lentivirus cannot replicate in the target cells and infect other cells.

The invention discloses a concentrated solution for selective preservation of legionella and application of the concentrated solution, and belongs to the technical field of microbiological detection. The concentrated solution provided by the invention is formed by synergistically combining the following components: a) an HEPES buffer system; b) a selective inhibitor combination comprising glycine, a cephalosporin antibiotic, a glycopeptide antibiotic, polymyxin B and an antifungal agent; c) a legionella physiological activity protective agent composition which comprises sodium pyruvate, betaine and L-cysteine; and d) a storage stabilizer combination comprising trehalose and a chemically modified cyclodextrin. The concentrated solution disclosed by the invention can efficiently and selectively inhibit infectious microbes in sample collection and sample preservation stages, and meanwhile, the physiological activity of legionella is effectively protected.

The invention belongs to the technical field of biology and the technical field of environment disinfection, and provides an anti-legionella preparation containing wood frog antibacterial peptide and application of the anti-legionella preparation. The preparation provided by the invention takes the wood frog antibacterial peptide as an effective component, is safer than a chemical disinfectant, has no irritation and metal corrosion problems, achieves a similar legionella killing effect, and is especially suitable for legionella disinfection in air conditioners, rooms and vehicles.

A process for detecting viable waterborne pathogens in a water sample, includes enriching at least a portion of the sample with an enrichment broth and taking a first DNA extract at time T0 and a second DNA extract at time T2 after an incubation period. Real-time polymerase chain reaction performed on the DNA extracts yields respective cycle threshold values Ct. The change in Ct provides an indication of viability of the targeted pathogen in the sample. An order of magnitude quantification of the sample can also be performed using a serial dilution technique on the T0 DNA extract. The process has particular application for detecting Legionella, including viable but not culturable cells.

The present invention relates to a method for determining the risk index for growth of Legionella in water systems of a building, said risk index being expressed as a numerical value, said method comprising the following steps: I) measurement of at least two values corresponding to variables selected from the group comprising physical, physicochemical and biological characteristics of said water system, each of said values corresponding to a risk value; and II) calculation of the overall risk index from the risk values by means of weighted composition using automated means; characterized in that: the method comprises measuring bioactivity in the water system by means of at least one biosensor installed in said water system, and determining by automated means the difference between an expected bioactivity value and the one actually measured; and in that, in the calculation of the risk index in step II, said difference is used as one of said measured risk values from step I to determine the risk index.