5 results about "Legionella" patented technology

The application provides a quality control for detecting respiratory pathogen nucleic acid, which comprises any one or more of the following respiratory pathogens: coronavirus, influenza virus, adenovirus, Mycoplasma pneumoniae, Streptococcus pneumoniae, rhinovirus or / and Legionella pneumophila. The full-length genome sequence of the coronavirus is divided into 6 target fragments, each with a length of 4000-5500 bp. The quality control of the application covers all detection target sequences (or target points) of the coronavirus, has a wide coverage, comprehensively detects target points, and does not have the phenomenon of missed detection. The quality control of the application contains main respiratory pathogen infections, has a wide range, and uses real virus samples and lentivirus samples as raw materials, and has more accurate detection target points. The preparation of the coronavirus quality control of the application integrates the target gene sequence into the host genome through the lentivirus vector, knocks out the self-replication gene in the preparation process, and has a "self-inactivation" ability, so that the recombinant lentivirus cannot replicate in the target cells and infect other cells.

The invention belongs to the technical field of biology and the technical field of environment disinfection, and provides an anti-legionella preparation containing wood frog antibacterial peptide and application of the anti-legionella preparation. The preparation provided by the invention takes the wood frog antibacterial peptide as an effective component, is safer than a chemical disinfectant, has no irritation and metal corrosion problems, achieves a similar legionella killing effect, and is especially suitable for legionella disinfection in air conditioners, rooms and vehicles.

A process for detecting viable waterborne pathogens in a water sample, includes enriching at least a portion of the sample with an enrichment broth and taking a first DNA extract at time T0 and a second DNA extract at time T2 after an incubation period. Real-time polymerase chain reaction performed on the DNA extracts yields respective cycle threshold values Ct. The change in Ct provides an indication of viability of the targeted pathogen in the sample. An order of magnitude quantification of the sample can also be performed using a serial dilution technique on the T0 DNA extract. The process has particular application for detecting Legionella, including viable but not culturable cells.