Gene chip-based method for synchronously and rapidly detecting thirteen pathogenic microorganisms in water body

A technology for pathogenic microorganisms and detection methods, which is applied in the determination/inspection of microorganisms, biochemical equipment and methods, and resistance to vector-borne diseases. It can solve the problems of long time, amplification failure, non-specific products, etc. High specificity, simple operation and strong stability

Inactive Publication Date: 2012-10-03
LOGISTICAL ENGINEERING UNIVERSITY OF PLA +1
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  • Abstract
  • Description
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  • Application Information

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Problems solved by technology

Although PCR is a specific, sensitive, simple and rapid detection technology, but this method requires a long time for sample purification process, and once there is a very small amount of exogenous DNA contamination, false positive results may occur; multiple pairs of primers simultan...

Method used

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  • Gene chip-based method for synchronously and rapidly detecting thirteen pathogenic microorganisms in water body
  • Gene chip-based method for synchronously and rapidly detecting thirteen pathogenic microorganisms in water body
  • Gene chip-based method for synchronously and rapidly detecting thirteen pathogenic microorganisms in water body

Examples

Experimental program
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Embodiment

[0050] Taking the drinking water source water sources in the Three Gorges Reservoir area of the Yangtze River as the detection object, the water outlets of the five tap water plants in the Chongqing section of the Yangtze River Basin are selected for the water samples for water samples.The discharge port and landscape waters are collected. This method is used for detection. The water sample information is shown in the table below.

[0051] Table 3 water sample information

[0052] serial number Water sample name Collection point Collection location description 1 Yangtze River Drinking Water Source Water 1 A water plant takes water points At 500m upstream, the water flow by the river is smooth, and the water is taken at 20cm underwater 2 Changjiang Drinking Water Source Water 2 Take a water point for a drink station At 500m upstream, the water flow by the river is smooth, and the water is taken at 20cm underwater 3 Changjiang Drinking Water Source Wat...

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PUM

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Abstract

The invention discloses a gene chip-based method for synchronously and rapidly detecting thirteen pathogenic microorganisms in a water body. The method is characterized in that specific gene segments of the pathogenic microorganisms are amplified simultaneously by adopting multiple polymerase chain reactions (PCR), and then, a detection result is obtained through gene chip hybridization and chip scanning image analysis. The detected thirteen pathogenic microorganisms comprise escherichia coli, Enterohemorrhagic Escherichia coli O157:H7, legionella pneumophila, salmonella enteritidis, Shigella, staphylococcus aureus, Listeria monocytogenes, Helicobacter Pylori, mycobacterium tuberculosis, Klebsiella pneumonia, vibrio cholera, Bacillus anthracis and Yersinia pestis.

Description

Technical field [0001] The invention is environmental science and engineering technology. Background technique [0002] At this stage, the methods commonly used at home and abroad to detect and identify pathogenic microorganisms mainly include traditional biochemical appraisal, immunology detection technology, PCR and other simple molecular biology and immunology methods. [0003] The traditional biochemical appraisal method (such as separation training, biochemical appraisal, etc.) is the most widely used. It is the national standard testing procedure such as "Food Sanitary Microbiology (GB / T 4789-2003)".The qualitative and quantitative results, but most of them need to go through 4 steps: pre -bacterial increase, bacterial increase, selective tablet separation, biochemical test and serum scientific types of identification. The entire process usually takes 3 to 7 days.The detected bacterial proliferation is a visible colonial. In addition, the detection of qualifications, bacter...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12Q1/14C12Q1/10C12Q1/04
CPCY02A50/30
Inventor 方振东张春秀谢朝新敖漉王大勇马颖陈金丝
Owner LOGISTICAL ENGINEERING UNIVERSITY OF PLA
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