30 results about "Shigella" patented technology

The invention relates to a bacteriophage, in particular to a cross-host multivalent bacteriophage and preparation and application of a bacteriostatic agent of the bacteriophage, the multivalent bacteriophage is preserved in the China Center for Type Culture Collection, and the preservation number is CCTCC NO: M 2026045. The salmonella bacteriophage CCTCC NO: M 2026045 is found for the first time, is a multivalent bacteriophage, can cross-host infect and split multiple important livestock and poultry intestinal pathogens such as salmonella, escherichia coli, shigella, klebsiella pneumoniae and proteus mirabilis, solves the problem of narrow host range of a single bacteriophage, and improves the prevention and control efficiency. By inhibiting the overgrowth of the gram-negative pathogenic bacteria, the intestinal microecological balance can be regulated, so that the aim of effective prevention and control is fulfilled.

The present application belongs to the technical field of wastewater treatment, and particularly relates to a seawater breeding wastewater antibiotic removal method based on oyster shell composite filler. Fine gravel, oyster shell and functional mineral are compounded in a fixed volume ratio of 2:1:1, and are laid in a structure with a limited particle size range and homogeneity without stratification. A salinity-directed salt-tolerant domestication process and a tidal intermittent operation mode of 48h hydraulic retention + 24h emptying and reoxygenation are matched, and salt-tolerant functional bacterial flora of Proteobacteria, Paracoccus and Shigella are enriched. The present application can adapt to a wide range of salinity conditions of 10‰-30‰, relieve high salt inhibition and ion competition, simultaneously and efficiently remove three typical antibiotics of conventional pollutants, sulfamethoxazole, trimethoprim and enrofloxacin, and reduce resistance genes. The sulfamethoxazole removal rate can reach 93.94% under the condition of 30‰ high salinity, the system runs stably and has strong adaptability, and has certain engineering application value and large-scale popularization potential in the field of seawater breeding wastewater treatment.

The present invention provides protected tetrasaccharides, their process of preparation and their use as common scaffold precursors in the synthesis of oligosaccharides representing segments of the O-antigens from diverse S. flexneri serotypes, comprising for example SF2a, SF3a, SF2b, SF1b, SFX and / or SFY.

The invention relates to a primer probe group, a multiplex fluorescent quantitative PCR method and a kit for simultaneously detecting six pathogenic bacteria, and belongs to the technical field of molecular biology, the primer probe group comprises primer sequences and probe sequences shown in SEQ ID No.1-18 in a table 1; the 5'end of each probe sequence is modified with a reporter group, and the 3 'end of each probe sequence is modified with a quenching group; the reporter group is Cy5, FAM and VIC, and the quenching group is Eclipse. The invention provides a method for simultaneously detecting six pathogenic bacteria including vibrio parahaemolyticus, salmonella, staphylococcus aureus, escherichia coli O157: H7, listeria monocytogenes and shigella in the same reaction system by combining a multiple real-time fluorescent quantitative PCR (Polymerase Chain Reaction) technology. The multiplex real-time fluorescent quantitative PCR kit provided by the invention is used for detecting the six pathogenic bacteria, can improve the detection sensitivity and specificity, greatly improves the detection efficiency, and overcomes the defects of complex steps, long detection period and incapability of adapting to large-scale rapid detection in the conventional detection method.

The invention provides a phosphorus-solubilizing synthetic microbiome as well as a preparation method and application thereof, and particularly belongs to the technical field of synthetic microbiome. The phosphorus-solubilizing synthesis microbiome comprises the following microorganisms: bacillus subtilis with the preservation number of CGMCC (China General Microbiological Culture Collection Center) No.36218, bacillus subtilis with the preservation number of CGMCC No.36218, bacillus subtilis with the preservation number of CGMCC No.36218, bacillus subtilis with The preservation number of the serratia marcescens is CGMCC (China General Microbiological Culture Collection Center) No.36217, and the preservation number of the serratia marcescens is CGMCC No.36217. And Shigella flexneri, the preservation number of which is CGMCC (China General Microbiological Culture Collection Center) No.36219, and the preservation number of the Shigella flexneri is CGMCC No.36219. The phosphorus-solubilizing synthetic microbiome has a phosphorus-solubilizing effect, can realize the improvement of the simulated lunar soil, particularly can be used for dissolving insoluble phosphorus in the simulated lunar soil, and can promote the growth of crops in the simulated lunar soil.

This invention discloses a quadruple Taqman qPCR reagent for detecting foodborne pathogens in aquatic products. The foodborne pathogens are Vibrio parahaemolyticus, Vibrio vulnificus, Shigella, and Staphylococcus aureus. The reagent includes specific primers and Taqman probes corresponding to the four foodborne pathogens. This invention provides a quadruple Taqman qPCR reagent with high efficiency, accuracy, sensitivity, and speed, offering an accurate, rapid, efficient, and economical method for detecting the above four foodborne pathogens in the quality and safety testing of aquatic products and their processed products. This is of great significance for ensuring the safety of aquatic products for consumption in my country.

The invention relates to the technical field of microorganisms and biological medicines, in particular to triple probiotics as well as a preparation method and application thereof, and the triple probiotics comprise at least one probiotic of bacillus, lactobacillus acidophilus and bifidobacterium longum. The triple probiotics provided by the invention form an'oxygen consumption-enzyme production-acid production 'synergistic chain, and through synergistic degradation of uric acid, inhibition of excessive growth of conditioned pathogens and enhancement of intestinal barrier functions, the blood uric acid level of patients with asymptomatic hyperuricemia is reduced, the alpha-diversity of intestinal tracts is improved, the abundance of butyric acid producing bacteria is improved, and the content of the butyric acid producing bacteria is improved. The ratio of escherichia coli to shigella is reduced; sIBO is effectively relieved or reversed, and digestive tract symptoms such as abdominal distension and exhaust are reduced. In addition, the triple probiotics avoid liver and kidney toxicity caused by chemical drugs, and are suitable for long-term administration.

The invention relates to the technical field of animal feed additives, in particular to a composition with bacteriostatic activity and application thereof. A screening antibacterial experiment of the plant essential oil and the organic acid shows that the composition of the plant essential oil and the organic acid, which is reasonably proportioned, has remarkable antibacterial activity on escherichia coli, salmonella typhimurium, shigella flexneri and clostridium perfringens, and particularly has a very good synergistic interaction effect. The composition of the plant essential oil and the organic acid is preferably thymol, carvacrol and sorbic acid, and the optimal compounding ratio is 3: 1: 1. The plant essential oil and organic acid composition provided by the invention has the advantages of being good in antibacterial effect, low in production cost, natural, non-toxic and the like, provides an effective treatment means for weaned piglets with bacterial diarrhea, and has huge potential commercial value.

The application discloses Slackia a new application of sp.D-G6, and particularly relates to Slackia application of sp.D-G6 bacterial powder in pig breeding, and belongs to the field of agricultural animal breeding and microbial technology. The bacterial strain has been preserved in the Guangdong Microbial Culture Collection Center on May 8, 2019, and the preservation number is GDMCC NO: 60661. Research shows that the bacterial powder has no obvious adverse effect on pigs, has good feeding safety, can promote pig feeding and weight gain, improve average daily feed intake and average daily weight gain, and improve growth performance. Further, the bacterial powder can regulate the microbial composition of the ileum of growing pigs, increase the abundance of Firmicutes and Lactobacillus, Lactobacillus and reduce the abundance of Proteobacteria and / or Escherichia-Shigella, Escherichia-Shigella and has application prospect of being developed into a new green feed additive.

The invention belongs to the technical field of biology, and particularly relates to a microbial marker combination for evaluating the quality of polygonatum sibiricum and application, the microbial marker combination comprises one or more of Aspergillus, Cercophora, Escherichia. Shigilla, Mycoplasm, Muribacaceae, Pseudomonas, Colletotrichum and Asaia, a method and an evaluation model for evaluating the quality of polygonatum sibiricum are established by utilizing the microbial marker combination, the sensitivity is 100.0%, the specificity is 72.7%, the AUC is 89.8%, and the evaluation model is used for evaluating the quality of polygonatum sibiricum. The method has high accuracy, specificity and sensitivity, and the evaluation method is economical, practical and easy and convenient to operate and can be widely applied to quality evaluation of rhizoma polygonati.

The invention establishes a method for simultaneously detecting vibrio parahaemolyticus, staphylococcus aureus, listeria monocytogenes, salmonella, shigella, bacillus cereus and vibrio alginolyticus on the basis of multiple PCR (Polymerase Chain Reaction) combined with a gene chip technology. The method comprises the following steps: selecting a heat-labile hemolysin gene of vibrio parahaemolyticus, a heat-resistant nuclease gene of staphylococcus aureus, a coded hemolysin O gene hlyA of listeria monocytogenes, an invasive protein A gene of salmonella enteritidis and an invasive plasmid antigen H gene of Shigella to carry out quintuple PCR (Polymerase Chain Reaction) amplification; bacillus cereus non-hemolytic toxin genes and vibrio alginolyticus similar Rpos sigma factor genes are selected for double PCR, two groups of multiplex PCR amplification products are hybridized with specific probes on the chip at the same time, and seven food-borne pathogenic bacteria can be detected at the same time. The method for detecting pathogenic bacteria by using the gene chip is high in specificity, high in sensitivity and strong in reliability.

Methods comprising obtaining a biological sample and extracting nucleic acids from it are disclosed. The methods process the nucleic acids for DNA sequencing and identify in the DNA sequencing data a plurality of amplicon sequence variants (ASV). The methods record the relative abundance of each ASV and classify, using a machine learning algorithm, each ASV according to one of a set of taxonomies. The set of taxonomies includes a pathobiont bacteria subset consisting of genus level groups Eggerthella group, Ruminococcus torques group, Ruminococcus gnavus group, Coprobacillus group, Streptococcus group, Bilophila group, Actinomyces group, Desulfovibrio group, Atopobiaceae group, Veillonella group, Enterococcus group, Escherichia-Shigella group, Campylobacter group, Enterobacteriaceae group, and Fusobacterium group. Based on the relative abundance of the ASV classified as pathobiont bacteria, a numerical indication of the health of the subject is calculated. Based on the numerical indication, the methods deliver to the subject a customized intervention.

The application belongs to the technical field of biotechnology and particularly relates to a lactobacillus gasseri LTG1323 and application thereof.The lactobacillus gasseri has strong abilities of bacteria multiplication and acid production, has strong inhibiting abilities on escherichia coli, staphylococcus aureus, bacillus cereus, shigella flexneri and salmonella typhimurium, has strong abilities of colonization in human intestinal tract, can regulate and optimize microenvironment, is sensitive to 6 common types of antibiotics, has high safety, and the prepared freeze-dried powder has characteristics of high number of viable bacteria and long shelf life.

The application discloses a kind of shigella bacteria specific protein antibodies and its application.The antibody is monoclonal antibody C65 and monoclonal antibody A61, monoclonal antibody C65 includes light chain variable region and heavy chain variable region, and the amino acid sequences of light chain variable region and heavy chain variable region are respectively shown as SEQ ID NO:7 and 8;Monoclonal antibody A61 includes light chain variable region and heavy chain variable region, and the amino acid sequences of light chain variable region and heavy chain variable region are respectively shown as SEQ ID NO:9 and 10.The magnetic bead antibody conjugate of the application is coupled by monoclonal antibody C65, monoclonal antibody A61 and activated carboxyl magnetic bead respectively.The magnetic bead antibody conjugate of the application is specific to shigella bacteria, and enrichment efficiency is high, and enrichment operation is simple, can be applied to shigella bacteria metabolite analysis, and also can be used for its qualitative and quantitative analysis.

Described herein are compositions, methods, and kits for detecting diarrhea-causing pathogens from a patient, food, or environmental sample. One embodiment described herein is a primer pair and probe for a multiplex polymerase chain reaction (PCR)-based assay for the detection of diarrhea-causing pathogens, such as Campylobacter spp., Salmonella spp., Shigella spp. / Enteroinvasive Escherichia coli (EIEC), Escherichia coli stx1 / Shiga toxin A, or Escherichia coli stx2 / Shiga toxin B. Other embodiments include methods and kits for detecting diarrhea-causing pathogens.

The invention belongs to the technical crossing field of microbiomics, and relates to a method for constructing a steady-state microbiome on the surface of a pear based on serratia rubra to inhibit postharvest diseases. The method comprises the following steps: firstly, preparing serratia rubra B11 suspension, and spraying the serratia rubra B11 suspension on the surfaces of pear fruits; the method comprises the following steps: standardly collecting epiphytic microorganisms on the surfaces of fruits by adopting a mode of combining physical elution and vacuum filtration, extracting total DNA, respectively carrying out high-throughput amplicon sequencing on a 16S rRNA gene V3-V4 region and a fungus ITS2 region, and carrying out dynamic analysis on alpha diversity, beta diversity, community composition and key class groups, so as to determine the content of the epiphytic microorganisms on the surfaces of the fruits. And finally, associating with the fruit rotting rate and the physiological quality index, establishing a set of full-process standardized system of multi-dimensional bioinformatics analysis, and revealing an ecological mechanism that beneficial bacteria realize disease inhibition by remodeling a fruit surface microbial community structure and regulating and controlling key function class groups. And reliable technical support is provided for mechanism research, efficiency evaluation and application development of the biocontrol microbial inoculum.

The invention discloses application of enzyme powder prepared from bamboo shoots to improvement of ulcerative colitis, which comprises the following steps: S1, pretreatment culture and grouping: 72 C57BL / 6J male mice are adaptively fed for one week and then are divided into 6 groups, each group comprises 12 male mice, and the number of the male mice is 12; the method comprises the following steps: sequentially naming a normal control group (NC), a model group (DSS), a sulfasalazine group (SASP, 300 mg / kg), a square bamboo shoot enzyme low-dose group (CUJ-L), a square bamboo shoot enzyme medium-dose group (CUJ-M) and a square bamboo shoot enzyme high-dose group (CUJ-H); experimental data of mice show that the square bamboo shoot enzyme treatment can significantly improve colon shortening and DAI, improve weight loss and reduce the colitis symptom factor level; besides, the chimonobambusa quadrangularis ferment intervention improves the diversity and the structure of intestinal bacteria, the composition of intestinal microbiota is remodeled, the abundance of Firmicite gate is improved, and the abundance of Proteobacteria and Verrucomicrobiota, including Escherichia-Shiga, Akkermansia and other species, is reduced, and the application of the chimonobambusa quadrangularis ferment intervention in the preparation of the chimonobambusa quadrangularis ferment intervention in the preparation of the chimonobambusa quadrangularis ferment intervention in the preparation of the chimonobambusa quadrangularis ferment intervention in the preparation of the chimonobambusa quadrangularis ferment is promoted. The intestinal metabolite and the intestinal flora have a close relationship; according to the method, 32 key metabolites are screened out through cecum metabolite analysis, and the key metabolites mainly relate to a Propionate metabolite metabolic pathway; the result shows that the square bamboo shoot enzyme has the potential of treating colitis.

The present invention provides protected tetrasaccharides, their process of preparation and their use in the synthesis of oligosaccharides, in particular fragments of O-antigens from Shigella flexneri, for example of serotype 1a, 1b, 2a, 2b, 3a, X, 4a, 4b, 5a, 5b, 7a or 7b.

The invention discloses a strain of shigella flexneri CY16, which is classified and named as shigella flexneri, and the preservation number of the shigella flexneri CY16 is CGMCC (China General Microbiological Culture Collection Center) No.33215. The strain provided by the invention can effectively reduce the morbidity of bacterial wilt of tomatoes, and has an effect of promoting the growth of tomato plants.

This invention relates to the field of microbial technology, and particularly to Pichia pastoris JSL-7 and its applications. The JSL-7 strain of this invention was isolated from cattle. Testing showed that this strain exhibits good antibacterial effects against Escherichia coli, Staphylococcus aureus, and / or Shigella flexneri. Furthermore, this strain is non-toxic in vivo, possesses good bile salt and acid resistance, and can colonize well in the animal's gastrointestinal tract, exhibiting good beneficial bacteria properties. In addition to its beneficial bacteria properties, analysis of its metabolites revealed that this strain can produce menthol without genetic modification, a characteristic not found in existing strains. Furthermore, through optimization of the fermentation medium, the optimal fermentation medium for the preparation of menthol by this strain was determined. This discovery can effectively shorten the production time of menthol, simplify the production process, and provide new strains and ideas for the future fermentation production of menthol.

The present invention relates to highly vesiculating Shigella strains. Highly vesiculating Shigella strains are produced by disrupting one or more components of the Tol-pal system. The blebs derived from these strains are useful immunogens in vaccines. Individual proteins present in these blebs can also be used as immunogens.

This invention provides a strain of Bacillus mojavensis, belonging to the field of microbial technology. The Bacillus mojavensis VT275 of this invention, with accession number CGMCC No. 29974, can effectively inhibit common skin pathogens and foodborne pathogens, such as Staphylococcus aureus, methicillin-resistant Staphylococcus aureus, Staphylococcus epidermidis, Listeria monocytogenes, Shigella, and Enterococcus faecalis. The Bacillus mojavensis VT275 of this invention can be used to prevent or treat skin diseases such as atopic dermatitis or to control foodborne infections, and has good application prospects in the fields of pharmaceuticals, cosmetics, edible microorganisms, and feed microorganisms.

The purpose of the (Fort's Gloves, Dry and Hold Pail) is to prevent moisture that causes the spread of bacteria from restrooms that is the cause of influenza, streptococcus, E coli, Hepatitis, MRSA, Salmonella, Shigella, and Norovirus. The pail is a new creation except for the authentic manuscript and cursive text. There is a distinct difference from the usual and traditional utility pails each community uses, because of the holder for the moisture elimination products that prevents contaminated water from spilling to the floor. Fecal and Trash water could potentially cause mildew growth on the gloves and the pail. The Dry & Hold container overall is a new Invention and a cleaner choice for the environment because the design prevents disease, foul odors.

The invention relates to the technical field of biological detection, and discloses an oligonucleotide composition, a kit and application. The composition disclosed by the invention can be used for rapidly and simultaneously detecting salmonella, shigella, entamoeba histolytica, vibrio cholerae, hepatitis A virus and hepatitis E virus in a sample with high specificity, and has relatively high sensitivity and stability. Compared with the traditional detection methods such as pathogen separation, genome sequencing and the like, the efficiency of identifying and detecting salmonella, shigella, entamoeba histolytica, vibrio cholerae, hepatitis A virus and hepatitis E virus by adopting the composition disclosed by the invention is effectively improved, the detection process is simple and convenient to operate, and the composition is suitable for large-scale popularization and application.

The invention discloses a probiotic compound preparation which is composed of probiotics loaded with biomass carbon dots. The probiotic compound preparation provided by the invention utilizes the biomass carbon dots to carry out functional modification on the probiotics, and compared with single use of lactobacillus reuteri, the compound preparation can significantly improve the survival rate of thalli in a harsh digestive tract environment (gastric acid and bile salt), effectively ensures that enough viable bacteria reach intestinal tracts and realize colonization, and improves the survival rate of the thalli in the harsh digestive tract environment (gastric acid and bile salt). The problem that a traditional probiotic preparation is low in efficiency is solved, the synergistic interaction of the carbon dots and the lactobacillus reuteri is achieved, and the L.r-coated CDs show multiple intestinal micro-ecological regulation functions.

This invention relates to the field of peptide preparation technology, specifically disclosing an immune-enhancing polypeptide derived from *Houttuynia cordata*, its preparation method, and its applications. The amino acid sequence of the polypeptide is shown in SEQ ID NO.1. This invention obtains the polypeptide through enzymatic extraction, chromatographic separation and purification, activity tracking screening, and mass spectrometry identification. Zebrafish experiments have confirmed that this polypeptide can significantly increase the activities of alkaline phosphatase, acid phosphatase, superoxide dismutase, and lysozyme content, exhibiting excellent immune-enhancing activity. In a *Shigella spp.* challenge experiment, the survival rate of zebrafish in the 200 mg / kg dose group reached 90.00%, far exceeding the 6.67% of the model control group. The *Houttuynia cordata*-derived immune-enhancing polypeptide of this invention has a well-defined structure and significant activity, and can be safely and efficiently used to enhance the immunity of aquatic animals and resist *Shigella spp.* infection, leaving no residue and unlikely to induce drug resistance, showing broad application prospects.

The invention discloses a leuconostoc mesenteroides exopolysaccharide for relieving diarrhea by directionally enriching intestinal beneficial bacteria, and belongs to the technical field of microorganisms. The exopolysaccharide is separated and purified from leuconostoc mesenteroides CCFM1516, is acidic heteropolysaccharide, is composed of galactosamine, glucosamine, galactose, glucose, mannose, galacturonic acid and glucuronic acid, is uniform in molecular weight distribution, and contains alpha-glucosidic bonds. The extracellular polysaccharide disclosed by the invention has a targeted regulation effect on intestinal flora, can be used for directionally enriching potential beneficial bacteria such as gParabacteroides, gParasuttera, gBacteroides and the like, can be used for reducing the abundance of a harmful bacterium gEscherichia-Shigiella, and has an important application value.

Technologies for the prevention and / or treatment of Shigella infections.

The invention belongs to the technical field of biology, and particularly relates to a microbial marker combination for evaluating the genuine nature of polygonatum sibiricum and application, the microbial marker combination is composed of Escherichia. Shigilla, Acremonium, Pseudomonas, Serratia, Mortierella, Wallemia, Engydonium, Sporidesmium, Ceratobasidium, Clonostachys and Ralstachys, an evaluation method for the genuine nature of polygonatum sibiricum is established, the sensitivity is 91.7%, the specificity is 100.0%, the AUC is 93.8%, and the method has the advantages of being high in sensitivity, strong in specificity and high in accuracy and can be used for evaluating the genuine nature of polygonatum sibiricum. The method can be used for judging whether the production area of the polygonatum sibiricum is a genuine Suhua production area or not, and can be widely applied to genuine evaluation of the polygonatum sibiricum.

This invention discloses a dual-channel quadruple ddPCR detection method and kit for the simultaneous detection of diarrheal bacteria in wastewater based on a proportional fluorescent probe strategy. Compared with traditional quadruple detection methods using four different fluorescent channels, the quadruple ddPCR detection method of this invention achieves quadruple detection only through the difference in probe concentration within two fluorescent channels. Wastewater, as a key interface between excrement and the environment, is not only an important site for the storage and transmission of pathogens but also a sentinel for early warning. The dual-channel quadruple ddPCR method established in this invention can simultaneously detect Salmonella in wastewater. Salmonella spp., SM), Shigella ( Shigella spp., SH), Vibrio parahaemolyticus ( V.parahaemolyticus, VP) and Vibrio cholerae ( Vibrio cholerae, VC (Vacuum-Containing Microorganisms) not only improves wastewater monitoring technology and methods, but also enables the detection of potential epidemic risks before disease outbreaks occur, allowing for timely implementation of public health prevention and control measures.