104 results about "Shigella" patented technology

The invention belongs to the field of plant endophytic fungus secondary metabolites, particularly relates to a secondary metabolite of fennel endophytic fungus fusarium oxysporum as well as a preparation method and application thereof, and provides a fennel endophytic fungus secondary metabolite with structures as shown in compounds 1-8. Wherein the compound 2 and the compound 7 have inhibitory activity on staphylococcus aureus, escherichia coli, bacillus cereus, bacillus subtilis, candida albicans and shigella, and have a certain inhibitory effect on cervical cancer Hela cell strains.

The present invention relates to Shigella outer membrane vesicles comprising at least one heterologous O-antigen, a recombinant Shigella bacterium comprising at least one heterologous O-antigen, methods for preparing the outer membrane vesicles or recombinant Shigella bacterium, plasmids for use in the methods, immunogenic compositions, vaccines and methods of treatment.

The invention relates to the technical field of microorganisms, and discloses an induction method of VBNC state shigella, a resuscitation medium and application, and the resuscitation medium comprises Rpf recombinant protein, MgSO4, sodium pyruvate, vitamin B12, tryptone, glucose, dipotassium phosphate, monopotassium phosphate, sodium chloride and Tween 80. The resuscitation culture medium obtained by the invention can be used for successfully culturing Shigella in a VBNC state.

The invention discloses stropharia rugoso-annulata polysaccharide as well as a preparation method and application thereof. The monosaccharide of the stropharia rugoso-annulata polysaccharide disclosed by the invention comprises glucose, galactose, mannose and galacturonic acid; the weight-average molecular weight of the stropharia rugoso-annulata polysaccharide is 1.4712 * 10 < 4 > to 1.623 * 10 < 4 > Da; the main chain of the stropharia rugoso-annulata polysaccharide contains-> 6)-alpha-D-(O-Me)-Galp-(1->, 1, 6-Glc, 1, 3-Man and 1.3-Gal exist in the main chain or a side chain, and terminal residues contain T-Glc and T-Gal. According to the invention, by constructing a DSS induced UC mouse model, the influence of Stropharia rugosoannulata polysaccharide (SRP1) on UC mice is investigated. Results show that the stropharia rugoso-annulata polysaccharide has a certain treatment effect on ulcerative colitis. In-vivo experiments show that the stropharia rugoso-annulata polysaccharide disclosed by the invention can increase abundance of bacteroides and thick-wall funguses and reduce abundance of proteobacteria and deferribacterium funguses; in-vitro experiments show that the stropharia rugoso-annulata polysaccharide can increase the abundance of beneficial bacteria bacteroides, reduce the abundance of harmful bacteria Escherichia-Shigella and Enterococcus, and increase the content of short-chain fatty acids.

The invention relates to the technical field of microorganisms, in particular to bacillus licheniformis JSF-9 and a complex microbial inoculant and application thereof.The strain JSF-9 is obtained by being separated from healthy cattle rectum contents by a research group, and it is detected that the strain has the good beta-1, 4-glucanase producing capacity, and the beta-1, 4-glucanase producing capacity is high. The antibacterial effect on staphylococcus aureus, salmonella typhimurium and shigella flexneri is achieved; the strain has a good bagasse fermentation capability, for example, after the strain, bacillus altitudinis MLF-1 and bacillus safensis MLL-5 are prepared into a mixed microbial inoculum, the bagasse fermentation capability is better, and verification shows that the complex microbial inoculum can effectively inhibit the reproduction of harmful bacteria, so that the preservation period of fermented feed is prolonged, and the yield of the fermented feed is improved. Animal feeding experiments find that the nutritional value and the disease resistance of the complex microbial inoculant fermented bagasse are remarkably improved, 10-15% of the complex microbial inoculant fermented bagasse is added into basic ration, and the cost-reducing and effect-increasing effects are achieved for feeding Jersey cattle.

The invention provides a primer combination of a multiple detection kit for food-borne pathogenic bacteria and the multiple detection kit. The primer combination comprises probes and primers for detecting nine food-borne pathogenic bacteria: vibrio cholerae general type, vibrio cholerae O1 type, vibrio cholerae O139 type, vibrio parahaemolyticus, salmonella, yersinia enterocolitica, campylobacter, listeria monocytogenes and enterobacter sakakaakii, and the sequence is shown as SEQ1-31; the probe and the primer pair are used for detecting nine characteristic genes, namely a shigella universal type: ipaH, a diarrheogenic Escherichia coli universal type: eltA, sta3, sta and aggR, and enterohemorrhagic Escherichia coli: stx1, stx2, escV and rfbE, and the sequences of the probe and the primer pair are shown as SEQ32-64. According to the invention, by combining a digital PCR multiple coding technology, the detection of 12 food-borne pathogenic bacteria can be realized, and the sensitivity, the specificity and the accuracy are relatively good.

The invention discloses a composition for treating hyperuricemia nephropathy and application thereof, and belongs to the field of biological medicines.The composition is prepared from, by weight, 28-32 parts of rhizoma smilacis glabrae, 28-32 parts of yam rhizome, 13-17 parts of radix clematidis, 8-12 parts of peach kernels, 8-12 parts of flos carthami, 8-12 parts of herba lycopi, 18-22 parts of rhizoma alismatis, 28-32 parts of raw coix seeds, 28-32 parts of semen plantaginis, 13-17 parts of rhizoma atractylodis and 13-17 parts of pseudobulbus cremastrae seu pleiones. The composition can obviously reduce the uric acid level of a mouse with high uric acid nephropathy and improve kidney injury; meanwhile, intestinal flora of mice with the hyperuricemia nephropathy can be remarkably remodeled, abundance of beneficial bacteria Ackerman's bacteria is up-regulated, abundance of opportunistic pathogenic bacteria such as escherichia coli-shigella, desulfurization vibrio and enterococcus is down-regulated, and important significance is achieved for research and drug development of the hyperuricemia nephropathy.

The invention belongs to the technical field of microorganisms, and discloses a plesiomonas shigelloides bacteriophage with cross-genus splitting decomposition capacity and application of the plesiomonas shigelloides bacteriophage, the plesiomonas shigelloides bacteriophage is classified and named as Plesiomonas virus, the plesiomonas shigelloides bacteriophage is preserved in China General Microbiological Culture Collection Center on March 21, 2025, and the preservation number is CGMCC No.46409; the plesiomonas shigelloides bacteriophage has a splitting decomposition effect on both plesiomonas shigelloides and aeromonas veronii. The bacteriophage has a high cracking rate on plesiomonas shigelloides and also has a cracking effect on aeromonas veronii, is a bacteriophage with cross-genus-species cracking, and is good in alkali resistance stability and good in thermal stability. The compound can be prepared into drugs, environment disinfectants, water additives and the like for preventing and treating plesiomonas shigelloides diseases and / or aeromonas veronii diseases of cultured fishes, so that the use of antibiotics is reduced.

The invention relates to a bacteriophage, in particular to a cross-host multivalent bacteriophage and preparation and application of a bacteriostatic agent of the bacteriophage, the multivalent bacteriophage is preserved in the China Center for Type Culture Collection, and the preservation number is CCTCC NO: M 2026045. The salmonella bacteriophage CCTCC NO: M 2026045 is found for the first time, is a multivalent bacteriophage, can cross-host infect and split multiple important livestock and poultry intestinal pathogens such as salmonella, escherichia coli, shigella, klebsiella pneumoniae and proteus mirabilis, solves the problem of narrow host range of a single bacteriophage, and improves the prevention and control efficiency. By inhibiting the overgrowth of the gram-negative pathogenic bacteria, the intestinal microecological balance can be regulated, so that the aim of effective prevention and control is fulfilled.

The invention provides bacillus mojavensis and belongs to the technical field of microorganisms. The preservation number of the bacillus mojavensis VT275 is CGMCC (China General Microbiological Culture Collection Center) No.29974. The bacillus mojavensis VT275 can be used for effectively inhibiting common skin pathogenic bacteria and food-borne pathogenic bacteria, such as staphylococcus aureus, methicillin-resistant staphylococcus aureus, staphylococcus epidermidis, listeria monocytogenes, shigella, enterococcus faecalis and the like. The bacillus mojavensis VT275 disclosed by the invention can be used for preventing or treating skin diseases such as specific dermatitis or controlling food-borne infection, and has a good application prospect in the fields of medicines, cosmetics, edible microorganisms, feed microorganisms and the like.

The invention relates to a primer and probe combination, a kit and a method for detecting gastrointestinal tract pathogenic bacteria. The gastrointestinal pathogenic bacteria comprise vibrio cholerae, vibrio parahaemolyticus, salmonella, shigella, diarrheagenic escherichia coli, campylobacter, yersinia enterocolons, clostridium difficile, cronobacter sakazakii, aeromonas hydrophila, plesiomonas shigelloides and vibrio fluvialis. The primer and the probe group can be independently used for PCR amplification detection and can be combined in the same reaction system and reaction procedure for multiple PCR amplification detection, and the primers and the probes aiming at different pathogenic bacteria cannot interfere with each other during multiple PCR amplification detection. The primer and probe combination covers various types of gastrointestinal pathogenic bacteria, and is high in sensitivity, low in detection limit, good in specificity and wide in application prospect.

The invention discloses a detection method for detecting enteropathogenic bacteria, and relates to the field of molecular biology, specific genes are searched in genomes of salmonella, shigella, vibrio parahaemolyticus, campylobacter jejuni, diarrheagenic escherichia coli, vibrio cholerae and staphylococcus aureus, a beta-actin gene is adopted as a reference gene, and the enteropathogenic bacteria are detected. Primer and probe sequences are designed, a probe group consisting of the primer and probe sequences is used for carrying out real-time fluorescent PCR amplification reaction on nucleic acid in a detected sample, then melting curve analysis is carried out, and whether the pathogenic bacteria exist in the nucleic acid sequence to be detected or not can be judged according to a Tm value. The detection method provided by the invention has the advantages of rapidness, high throughput, accuracy and high sensitivity.

The invention relates to the technical field of microorganisms, in particular to Weissella fusiformis JWC120 with a function of inhibiting common harmful bacteria in animal breeding and application of the Weissella fusiformis JWC120. The Weissella fusiformis JWC120 with the function of inhibiting common harmful bacteria in animal breeding, provided by the invention, has the preservation number of CGMCC No.31945, and can effectively inhibit common escherichia coli, salmonella, staphylococcus aureus and shigella in animal breeding; a bacteriocin crude extract of the strain has a remarkable inhibition effect on common staphylococcus aureus in animal breeding, and has a good application prospect in animal disease prevention and treatment and development of livestock and poultry breeding health-care products.

The present application belongs to the technical field of wastewater treatment, and particularly relates to a seawater breeding wastewater antibiotic removal method based on oyster shell composite filler. Fine gravel, oyster shell and functional mineral are compounded in a fixed volume ratio of 2:1:1, and are laid in a structure with a limited particle size range and homogeneity without stratification. A salinity-directed salt-tolerant domestication process and a tidal intermittent operation mode of 48h hydraulic retention + 24h emptying and reoxygenation are matched, and salt-tolerant functional bacterial flora of Proteobacteria, Paracoccus and Shigella are enriched. The present application can adapt to a wide range of salinity conditions of 10‰-30‰, relieve high salt inhibition and ion competition, simultaneously and efficiently remove three typical antibiotics of conventional pollutants, sulfamethoxazole, trimethoprim and enrofloxacin, and reduce resistance genes. The sulfamethoxazole removal rate can reach 93.94% under the condition of 30‰ high salinity, the system runs stably and has strong adaptability, and has certain engineering application value and large-scale popularization potential in the field of seawater breeding wastewater treatment.

The invention relates to the technical field of probiotics, in particular to lactobacillus rhamnosus FJS004 for inhibiting food-borne pathogenic bacteria, lactein of lactobacillus rhamnosus FJS004 and application of lactobacillus rhamnosus FJS004. The lactobacillus rhamnosus FJS004 is preserved in the China General Microbiological Culture Collection Center (CGMCC) on December 26, 2022, the preservation address is No.3, No.1 yard, Beichen West Road, Chaoyang District, Beijing, and the preservation number is CGMCC No.26253. The lactobacillus rhamnosus FJS004 can be used for preparing the lactobacillus rhamnosus FJS004. The lactobacillus rhamnosus FJS004 has the advantages that the lactobacillus rhamnosus FJS004 can be used for preparing the lactobacillus rhamnosus FJS004; the lactobacillus rhamnosus FJS004 and the lactein of the lactobacillus rhamnosus FJS004 provided by the invention have a good bacteriostatic or bactericidal effect on escherichia coli, salmonella, shigella, staphylococcus aureus, listeria monocytogenes, pseudomonas aeruginosa, bacillus cereus and enterobacter sakazakii.

The present invention discloses a method for detecting Shigella and its application. The method includes: identifying a sequence rich in AT bases in the Shigella genomic sequence as a target sequence; automatically and high-throughput designing specific primers for the target sequence; designing a specific Tm value calculation formula to calculate the reaction denaturation and annealing temperatures, and setting nucleic acid amplification reaction conditions accordingly; and performing nucleic acid amplification reaction under the condition of local strand separation of the template. The present invention targets sequences rich in AT bases that are usually avoided in traditional nucleic acid amplification methods, greatly broadening the candidate target regions; through specific screening based on a large amount of genomic data and precise calculation of the Tm value for AT-rich regions, local strand separation of double-stranded DNA is achieved, greatly reducing the possibility of non-specific amplification in nucleic acid amplification technology. This method effectively expands the application scope of traditional nucleic acid amplification technology and significantly improves the reaction performance.

The present invention provides protected tetrasaccharides, their process of preparation and their use as common scaffold precursors in the synthesis of oligosaccharides representing segments of the O-antigens from diverse S. flexneri serotypes, comprising for example SF2a, SF3a, SF2b, SF1b, SFX and / or SFY.

The invention relates to a primer probe group, a multiplex fluorescent quantitative PCR method and a kit for simultaneously detecting six pathogenic bacteria, and belongs to the technical field of molecular biology, the primer probe group comprises primer sequences and probe sequences shown in SEQ ID No.1-18 in a table 1; the 5'end of each probe sequence is modified with a reporter group, and the 3 'end of each probe sequence is modified with a quenching group; the reporter group is Cy5, FAM and VIC, and the quenching group is Eclipse. The invention provides a method for simultaneously detecting six pathogenic bacteria including vibrio parahaemolyticus, salmonella, staphylococcus aureus, escherichia coli O157: H7, listeria monocytogenes and shigella in the same reaction system by combining a multiple real-time fluorescent quantitative PCR (Polymerase Chain Reaction) technology. The multiplex real-time fluorescent quantitative PCR kit provided by the invention is used for detecting the six pathogenic bacteria, can improve the detection sensitivity and specificity, greatly improves the detection efficiency, and overcomes the defects of complex steps, long detection period and incapability of adapting to large-scale rapid detection in the conventional detection method.

Provided herein are compositions comprising in vivo succinate-producting microorganisms, e.g., Bacteroides and Prevotella species (preferably Bacteroides thetaiotaomicron (e.g., VPI 5482), Bacteroides vulgatus (e.g., NCTC 11154, Prevotella copri (e.g., DSM 18205)), Parabacteroides (e.g., Parabacteroides distasonis), and Bacilli (e.g. Lactobacillus animalis), and methods of use thereof in treating or reducing risk of developing an intestinal infection, optionally an infection with Campylobacter, Salmonella, E. coli, Shigella, Listeria monocytogenes, Vibrio, Enteropathogenic E. coli, Klebsiella, or Clostridioides difficile, or promoting expansion of colonic tuft cells, in a subject.

The invention discloses a nucleic acid detection kit for typhoid fever, paratyphoid fever, cholera, shigella and dysentery. The nucleic acid detection kit comprises a multiple fluorescent PCR detection system, and the multiple fluorescent PCR detection system comprises a typhoid fever salmonella primer probe, a paratyphoid fever salmonella primer probe, a vibrio cholera primer probe, a dysentery amoeba primer probe and a shigella primer probe; according to the kit, specific and conservative characteristic genes of different bacteria species are selected for primer probe design, so that the specificity, the accuracy and the sensitivity of detection are ensured.

The invention provides a phosphorus-solubilizing synthetic microbiome as well as a preparation method and application thereof, and particularly belongs to the technical field of synthetic microbiome. The phosphorus-solubilizing synthesis microbiome comprises the following microorganisms: bacillus subtilis with the preservation number of CGMCC (China General Microbiological Culture Collection Center) No.36218, bacillus subtilis with the preservation number of CGMCC No.36218, bacillus subtilis with the preservation number of CGMCC No.36218, bacillus subtilis with The preservation number of the serratia marcescens is CGMCC (China General Microbiological Culture Collection Center) No.36217, and the preservation number of the serratia marcescens is CGMCC No.36217. And Shigella flexneri, the preservation number of which is CGMCC (China General Microbiological Culture Collection Center) No.36219, and the preservation number of the Shigella flexneri is CGMCC No.36219. The phosphorus-solubilizing synthetic microbiome has a phosphorus-solubilizing effect, can realize the improvement of the simulated lunar soil, particularly can be used for dissolving insoluble phosphorus in the simulated lunar soil, and can promote the growth of crops in the simulated lunar soil.

The invention provides a kit for detecting shigella by a Marker method, and the Marker method represents fluorescence intensity corresponding to shigella with different concentrations and is a basis for establishing a Marker graph; the kit comprises a Marker graph, a magnetic aptamer, a detection aptamer, a bacterial lysis solution, an enzyme digestion solution, a binding solution, a washing solution and a magnetic frame. The detection method comprises the following steps: the kit is used for splitting the shigella through a bacterial lysis solution to release DNA, the DNA is cut through an enzyme digestion solution to obtain a fragment rich in an ipaH gene, the magnetic aptamer and the detection aptamer are specifically combined with the ipaH fragment under the action of a binding solution to form a compound, the compound is washed with washing liquid, the fluorescence intensity is detected and compared with a Marker diagram, and quantitative detection of the shigella is realized. The method does not need to treat a sample in advance, shortens the detection time, and has strong specificity and high sensitivity.

The present application relates to the field of agricultural husbandry and veterinary technology, in particular to a protease-resistant antibacterial peptide FR and application thereof. The present application provides a protease-resistant antibacterial peptide FR, and the amino acid sequence of the antibacterial peptide FR is shown as SEQ ID NO. 1. The results of the specific implementation of the present application show that the protease-resistant antibacterial peptide FR provided by the present application has the activity of resisting gram-negative bacteria (Escherichia coli, Shigella flexneri, Salmonella and Campylobacter jejuni, etc.), has low hemolytic activity, can resist the cleavage of high-concentration protease, and has very good application prospect. In summary, the antibacterial peptide FR provided by the present application has the advantages of resisting gastrointestinal environment, resisting high salt and resisting protease cleavage, can realize the killing effect on gram-negative bacteria in the presence of enzymes and salt ions, and has the characteristics of low hemolytic activity, thereby providing a new way for the development of antibiotic substitutes.

This application discloses a combination of gut microbial biomarkers, a system, and its application for detecting liver injury in fish, belonging to the field of molecular detection technology for fish liver injury. The gut microbial biomarker combination includes *Cetobacter* spp. (…). Cetobacterium ), Pseudomonas spp. Pseudomonas ) and Escherichia coli-Shigella spp. Escherichia‑Shigella Using the intestinal microbial biomarker combination described in this application, non-invasive diagnosis of liver damage can be achieved without touching the fish body, with high accuracy and no missed detections, especially avoiding misdiagnosis as liver damage that could lead to overtreatment. This provides a basis for judgment in aquaculture environment control and precise disease prevention and control, and is beneficial for improving the survival rate and profitability of yellow catfish farming.

The present disclosure provides a cell-free method for synthesizing an Invasion Plasmid Antigen B (IpaB) antigen associated with a Shigella bacterium comprising exogenous addition of the purified chaperone protein IpgC to the cell-free synthesis mixture. The disclosure further provides IpaB antigen mutants comprising non-natural amino acids incorporated during cell-free synthesis, enabling covalent conjugation to a Shigella O-antigen polysaccharide. Further provided are Ipa B antigens and conjugates thereof, as well as immunogenic compositions prepared with the synthesized IpaB antigens and conjugates thereof and methods of use.

The invention relates to a method and a system for identifying Shigella and / or diarrheagenic Escherichia coli. The method comprises the following steps: collecting mass spectrum data of known strains through paper spray ionization; generating a training sample based on mass spectrum data of a known strain; constructing a strain prediction model, and training the strain prediction model by using the training sample; collecting mass spectrum data of the strain to be detected through paper spray ionization; and predicting a to-be-detected strain by using the trained strain prediction model based on the mass spectrum data of the to-be-detected strain, and determining the type of the to-be-detected strain. The method and the system can directly identify the strain sample, do not need sample pretreatment, are low in analysis cost and simple and convenient to operate, can identify the strain category in high throughput and real time, and are high in identification result accuracy, high in reliability and high in identification speed.

The invention discloses a screening culture medium and a preparation method and application thereof, the screening culture medium comprises a basic culture medium and additives, and the additives comprise amphotericin B, vancomycin, ox bile salt, sodium deoxycholate and No.3 bile salt. According to the screening culture medium provided by the invention, a special infectious microbe inhibitor is added on the basis of a trisaccharide iron culture medium, so that most gram-negative enterobacter, staphylococcus aureus and part of fungi except salmonella enteritis subspecies and shigella cannot grow; the interference of the infectious microbes on the detection of the salmonella enteritis subspecies and the shigella is avoided, so that the fluorescent probe can be applied to the detection of the salmonella enteritis subspecies and the shigella in a clinical anus swab, and the sensitivity of color development is improved.

The invention discloses an intestinal microbial marker combination and system for detecting fish liver injury and application, and belongs to the technical field of molecular detection of fish liver injury. The invention relates to an enteric microbial marker combination, which is characterized in that the enteric microbial marker combination comprises the following components: cetyl, pseudomonas and Escherichia-Shigella, and the enteric microbial marker combination comprises the following components: bacillus, pseudomonas and Escherichia-Shigella, and the enteric microbial marker combination comprises the following components: bacillus, pseudomonas and Escherichia-Shigella. By utilizing the intestinal microbial marker combination, non-invasive diagnosis of liver injury can be realized on the premise of not invading a fish body, the accuracy is high, missing detection is avoided, and particularly, the condition that liver injury is misjudged to cause excessive treatment is avoided. A judgment basis is provided for breeding environment regulation and control and precise disease prevention and treatment, the breeding survival rate of the pelteobagrus fulvidraco is increased, and the breeding benefit is improved.

The invention relates to the technical field of microorganisms, in particular to pichia ethanol yeast JSL-7 and application thereof.The pichia ethanol yeast JSL-7 is obtained by being separated from the body of a cattle, it is detected that the strain has a good antibacterial effect on escherichia coli, staphylococcus aureus and / or shigella flexneri, in addition, the strain is free of in-vivo toxicity, and the strain has a good application prospect. The strain has good cholate resistance and acid resistance, can be well colonized in intestines and stomachs of animals and has good probiotic performance, besides the probiotic performance, through analysis of metabolites, it is found that the strain can produce menthol without gene modification, which is the characteristic that a strain in the prior art does not have, and the menthol can be produced by the strain without gene modification. And after a fermentation culture medium is optimized, an optimal fermentation culture medium for preparing menthol by fermenting the strain is also obtained, so that the production time of menthol can be effectively shortened, the production process is simplified, and a new strain and a new idea are provided for producing menthol by a fermentation method in the future.

This invention discloses a quadruple Taqman qPCR reagent for detecting foodborne pathogens in aquatic products. The foodborne pathogens are Vibrio parahaemolyticus, Vibrio vulnificus, Shigella, and Staphylococcus aureus. The reagent includes specific primers and Taqman probes corresponding to the four foodborne pathogens. This invention provides a quadruple Taqman qPCR reagent with high efficiency, accuracy, sensitivity, and speed, offering an accurate, rapid, efficient, and economical method for detecting the above four foodborne pathogens in the quality and safety testing of aquatic products and their processed products. This is of great significance for ensuring the safety of aquatic products for consumption in my country.