74 results about "Shigella" patented technology

The invention belongs to the field of plant endophytic fungus secondary metabolites, particularly relates to a secondary metabolite of fennel endophytic fungus fusarium oxysporum as well as a preparation method and application thereof, and provides a fennel endophytic fungus secondary metabolite with structures as shown in compounds 1-8. Wherein the compound 2 and the compound 7 have inhibitory activity on staphylococcus aureus, escherichia coli, bacillus cereus, bacillus subtilis, candida albicans and shigella, and have a certain inhibitory effect on cervical cancer Hela cell strains.

The present invention relates to Shigella outer membrane vesicles comprising at least one heterologous O-antigen, a recombinant Shigella bacterium comprising at least one heterologous O-antigen, methods for preparing the outer membrane vesicles or recombinant Shigella bacterium, plasmids for use in the methods, immunogenic compositions, vaccines and methods of treatment.

The invention discloses stropharia rugoso-annulata polysaccharide as well as a preparation method and application thereof. The monosaccharide of the stropharia rugoso-annulata polysaccharide disclosed by the invention comprises glucose, galactose, mannose and galacturonic acid; the weight-average molecular weight of the stropharia rugoso-annulata polysaccharide is 1.4712 * 10 < 4 > to 1.623 * 10 < 4 > Da; the main chain of the stropharia rugoso-annulata polysaccharide contains-> 6)-alpha-D-(O-Me)-Galp-(1->, 1, 6-Glc, 1, 3-Man and 1.3-Gal exist in the main chain or a side chain, and terminal residues contain T-Glc and T-Gal. According to the invention, by constructing a DSS induced UC mouse model, the influence of Stropharia rugosoannulata polysaccharide (SRP1) on UC mice is investigated. Results show that the stropharia rugoso-annulata polysaccharide has a certain treatment effect on ulcerative colitis. In-vivo experiments show that the stropharia rugoso-annulata polysaccharide disclosed by the invention can increase abundance of bacteroides and thick-wall funguses and reduce abundance of proteobacteria and deferribacterium funguses; in-vitro experiments show that the stropharia rugoso-annulata polysaccharide can increase the abundance of beneficial bacteria bacteroides, reduce the abundance of harmful bacteria Escherichia-Shigella and Enterococcus, and increase the content of short-chain fatty acids.

The invention relates to the technical field of microorganisms, in particular to bacillus licheniformis JSF-9 and a complex microbial inoculant and application thereof.The strain JSF-9 is obtained by being separated from healthy cattle rectum contents by a research group, and it is detected that the strain has the good beta-1, 4-glucanase producing capacity, and the beta-1, 4-glucanase producing capacity is high. The antibacterial effect on staphylococcus aureus, salmonella typhimurium and shigella flexneri is achieved; the strain has a good bagasse fermentation capability, for example, after the strain, bacillus altitudinis MLF-1 and bacillus safensis MLL-5 are prepared into a mixed microbial inoculum, the bagasse fermentation capability is better, and verification shows that the complex microbial inoculum can effectively inhibit the reproduction of harmful bacteria, so that the preservation period of fermented feed is prolonged, and the yield of the fermented feed is improved. Animal feeding experiments find that the nutritional value and the disease resistance of the complex microbial inoculant fermented bagasse are remarkably improved, 10-15% of the complex microbial inoculant fermented bagasse is added into basic ration, and the cost-reducing and effect-increasing effects are achieved for feeding Jersey cattle.

The invention provides a primer combination of a multiple detection kit for food-borne pathogenic bacteria and the multiple detection kit. The primer combination comprises probes and primers for detecting nine food-borne pathogenic bacteria: vibrio cholerae general type, vibrio cholerae O1 type, vibrio cholerae O139 type, vibrio parahaemolyticus, salmonella, yersinia enterocolitica, campylobacter, listeria monocytogenes and enterobacter sakakaakii, and the sequence is shown as SEQ1-31; the probe and the primer pair are used for detecting nine characteristic genes, namely a shigella universal type: ipaH, a diarrheogenic Escherichia coli universal type: eltA, sta3, sta and aggR, and enterohemorrhagic Escherichia coli: stx1, stx2, escV and rfbE, and the sequences of the probe and the primer pair are shown as SEQ32-64. According to the invention, by combining a digital PCR multiple coding technology, the detection of 12 food-borne pathogenic bacteria can be realized, and the sensitivity, the specificity and the accuracy are relatively good.

The invention discloses a composition for treating hyperuricemia nephropathy and application thereof, and belongs to the field of biological medicines.The composition is prepared from, by weight, 28-32 parts of rhizoma smilacis glabrae, 28-32 parts of yam rhizome, 13-17 parts of radix clematidis, 8-12 parts of peach kernels, 8-12 parts of flos carthami, 8-12 parts of herba lycopi, 18-22 parts of rhizoma alismatis, 28-32 parts of raw coix seeds, 28-32 parts of semen plantaginis, 13-17 parts of rhizoma atractylodis and 13-17 parts of pseudobulbus cremastrae seu pleiones. The composition can obviously reduce the uric acid level of a mouse with high uric acid nephropathy and improve kidney injury; meanwhile, intestinal flora of mice with the hyperuricemia nephropathy can be remarkably remodeled, abundance of beneficial bacteria Ackerman's bacteria is up-regulated, abundance of opportunistic pathogenic bacteria such as escherichia coli-shigella, desulfurization vibrio and enterococcus is down-regulated, and important significance is achieved for research and drug development of the hyperuricemia nephropathy.

The invention belongs to the technical field of microorganisms, and discloses a plesiomonas shigelloides bacteriophage with cross-genus splitting decomposition capacity and application of the plesiomonas shigelloides bacteriophage, the plesiomonas shigelloides bacteriophage is classified and named as Plesiomonas virus, the plesiomonas shigelloides bacteriophage is preserved in China General Microbiological Culture Collection Center on March 21, 2025, and the preservation number is CGMCC No.46409; the plesiomonas shigelloides bacteriophage has a splitting decomposition effect on both plesiomonas shigelloides and aeromonas veronii. The bacteriophage has a high cracking rate on plesiomonas shigelloides and also has a cracking effect on aeromonas veronii, is a bacteriophage with cross-genus-species cracking, and is good in alkali resistance stability and good in thermal stability. The compound can be prepared into drugs, environment disinfectants, water additives and the like for preventing and treating plesiomonas shigelloides diseases and / or aeromonas veronii diseases of cultured fishes, so that the use of antibiotics is reduced.

The invention relates to a bacteriophage, in particular to a cross-host multivalent bacteriophage and preparation and application of a bacteriostatic agent of the bacteriophage, the multivalent bacteriophage is preserved in the China Center for Type Culture Collection, and the preservation number is CCTCC NO: M 2026045. The salmonella bacteriophage CCTCC NO: M 2026045 is found for the first time, is a multivalent bacteriophage, can cross-host infect and split multiple important livestock and poultry intestinal pathogens such as salmonella, escherichia coli, shigella, klebsiella pneumoniae and proteus mirabilis, solves the problem of narrow host range of a single bacteriophage, and improves the prevention and control efficiency. By inhibiting the overgrowth of the gram-negative pathogenic bacteria, the intestinal microecological balance can be regulated, so that the aim of effective prevention and control is fulfilled.

The invention provides bacillus mojavensis and belongs to the technical field of microorganisms. The preservation number of the bacillus mojavensis VT275 is CGMCC (China General Microbiological Culture Collection Center) No.29974. The bacillus mojavensis VT275 can be used for effectively inhibiting common skin pathogenic bacteria and food-borne pathogenic bacteria, such as staphylococcus aureus, methicillin-resistant staphylococcus aureus, staphylococcus epidermidis, listeria monocytogenes, shigella, enterococcus faecalis and the like. The bacillus mojavensis VT275 disclosed by the invention can be used for preventing or treating skin diseases such as specific dermatitis or controlling food-borne infection, and has a good application prospect in the fields of medicines, cosmetics, edible microorganisms, feed microorganisms and the like.

The invention relates to the technical field of microorganisms, in particular to Weissella fusiformis JWC120 with a function of inhibiting common harmful bacteria in animal breeding and application of the Weissella fusiformis JWC120. The Weissella fusiformis JWC120 with the function of inhibiting common harmful bacteria in animal breeding, provided by the invention, has the preservation number of CGMCC No.31945, and can effectively inhibit common escherichia coli, salmonella, staphylococcus aureus and shigella in animal breeding; a bacteriocin crude extract of the strain has a remarkable inhibition effect on common staphylococcus aureus in animal breeding, and has a good application prospect in animal disease prevention and treatment and development of livestock and poultry breeding health-care products.

The present application belongs to the technical field of wastewater treatment, and particularly relates to a seawater breeding wastewater antibiotic removal method based on oyster shell composite filler. Fine gravel, oyster shell and functional mineral are compounded in a fixed volume ratio of 2:1:1, and are laid in a structure with a limited particle size range and homogeneity without stratification. A salinity-directed salt-tolerant domestication process and a tidal intermittent operation mode of 48h hydraulic retention + 24h emptying and reoxygenation are matched, and salt-tolerant functional bacterial flora of Proteobacteria, Paracoccus and Shigella are enriched. The present application can adapt to a wide range of salinity conditions of 10‰-30‰, relieve high salt inhibition and ion competition, simultaneously and efficiently remove three typical antibiotics of conventional pollutants, sulfamethoxazole, trimethoprim and enrofloxacin, and reduce resistance genes. The sulfamethoxazole removal rate can reach 93.94% under the condition of 30‰ high salinity, the system runs stably and has strong adaptability, and has certain engineering application value and large-scale popularization potential in the field of seawater breeding wastewater treatment.

The present invention discloses a method for detecting Shigella and its application. The method includes: identifying a sequence rich in AT bases in the Shigella genomic sequence as a target sequence; automatically and high-throughput designing specific primers for the target sequence; designing a specific Tm value calculation formula to calculate the reaction denaturation and annealing temperatures, and setting nucleic acid amplification reaction conditions accordingly; and performing nucleic acid amplification reaction under the condition of local strand separation of the template. The present invention targets sequences rich in AT bases that are usually avoided in traditional nucleic acid amplification methods, greatly broadening the candidate target regions; through specific screening based on a large amount of genomic data and precise calculation of the Tm value for AT-rich regions, local strand separation of double-stranded DNA is achieved, greatly reducing the possibility of non-specific amplification in nucleic acid amplification technology. This method effectively expands the application scope of traditional nucleic acid amplification technology and significantly improves the reaction performance.

The present invention provides protected tetrasaccharides, their process of preparation and their use as common scaffold precursors in the synthesis of oligosaccharides representing segments of the O-antigens from diverse S. flexneri serotypes, comprising for example SF2a, SF3a, SF2b, SF1b, SFX and / or SFY.

The invention relates to a primer probe group, a multiplex fluorescent quantitative PCR method and a kit for simultaneously detecting six pathogenic bacteria, and belongs to the technical field of molecular biology, the primer probe group comprises primer sequences and probe sequences shown in SEQ ID No.1-18 in a table 1; the 5'end of each probe sequence is modified with a reporter group, and the 3 'end of each probe sequence is modified with a quenching group; the reporter group is Cy5, FAM and VIC, and the quenching group is Eclipse. The invention provides a method for simultaneously detecting six pathogenic bacteria including vibrio parahaemolyticus, salmonella, staphylococcus aureus, escherichia coli O157: H7, listeria monocytogenes and shigella in the same reaction system by combining a multiple real-time fluorescent quantitative PCR (Polymerase Chain Reaction) technology. The multiplex real-time fluorescent quantitative PCR kit provided by the invention is used for detecting the six pathogenic bacteria, can improve the detection sensitivity and specificity, greatly improves the detection efficiency, and overcomes the defects of complex steps, long detection period and incapability of adapting to large-scale rapid detection in the conventional detection method.

Provided herein are compositions comprising in vivo succinate-producting microorganisms, e.g., Bacteroides and Prevotella species (preferably Bacteroides thetaiotaomicron (e.g., VPI 5482), Bacteroides vulgatus (e.g., NCTC 11154, Prevotella copri (e.g., DSM 18205)), Parabacteroides (e.g., Parabacteroides distasonis), and Bacilli (e.g. Lactobacillus animalis), and methods of use thereof in treating or reducing risk of developing an intestinal infection, optionally an infection with Campylobacter, Salmonella, E. coli, Shigella, Listeria monocytogenes, Vibrio, Enteropathogenic E. coli, Klebsiella, or Clostridioides difficile, or promoting expansion of colonic tuft cells, in a subject.

The invention provides a phosphorus-solubilizing synthetic microbiome as well as a preparation method and application thereof, and particularly belongs to the technical field of synthetic microbiome. The phosphorus-solubilizing synthesis microbiome comprises the following microorganisms: bacillus subtilis with the preservation number of CGMCC (China General Microbiological Culture Collection Center) No.36218, bacillus subtilis with the preservation number of CGMCC No.36218, bacillus subtilis with the preservation number of CGMCC No.36218, bacillus subtilis with The preservation number of the serratia marcescens is CGMCC (China General Microbiological Culture Collection Center) No.36217, and the preservation number of the serratia marcescens is CGMCC No.36217. And Shigella flexneri, the preservation number of which is CGMCC (China General Microbiological Culture Collection Center) No.36219, and the preservation number of the Shigella flexneri is CGMCC No.36219. The phosphorus-solubilizing synthetic microbiome has a phosphorus-solubilizing effect, can realize the improvement of the simulated lunar soil, particularly can be used for dissolving insoluble phosphorus in the simulated lunar soil, and can promote the growth of crops in the simulated lunar soil.

The invention provides a kit for detecting shigella by a Marker method, and the Marker method represents fluorescence intensity corresponding to shigella with different concentrations and is a basis for establishing a Marker graph; the kit comprises a Marker graph, a magnetic aptamer, a detection aptamer, a bacterial lysis solution, an enzyme digestion solution, a binding solution, a washing solution and a magnetic frame. The detection method comprises the following steps: the kit is used for splitting the shigella through a bacterial lysis solution to release DNA, the DNA is cut through an enzyme digestion solution to obtain a fragment rich in an ipaH gene, the magnetic aptamer and the detection aptamer are specifically combined with the ipaH fragment under the action of a binding solution to form a compound, the compound is washed with washing liquid, the fluorescence intensity is detected and compared with a Marker diagram, and quantitative detection of the shigella is realized. The method does not need to treat a sample in advance, shortens the detection time, and has strong specificity and high sensitivity.

The present application relates to the field of agricultural husbandry and veterinary technology, in particular to a protease-resistant antibacterial peptide FR and application thereof. The present application provides a protease-resistant antibacterial peptide FR, and the amino acid sequence of the antibacterial peptide FR is shown as SEQ ID NO. 1. The results of the specific implementation of the present application show that the protease-resistant antibacterial peptide FR provided by the present application has the activity of resisting gram-negative bacteria (Escherichia coli, Shigella flexneri, Salmonella and Campylobacter jejuni, etc.), has low hemolytic activity, can resist the cleavage of high-concentration protease, and has very good application prospect. In summary, the antibacterial peptide FR provided by the present application has the advantages of resisting gastrointestinal environment, resisting high salt and resisting protease cleavage, can realize the killing effect on gram-negative bacteria in the presence of enzymes and salt ions, and has the characteristics of low hemolytic activity, thereby providing a new way for the development of antibiotic substitutes.

This application discloses a combination of gut microbial biomarkers, a system, and its application for detecting liver injury in fish, belonging to the field of molecular detection technology for fish liver injury. The gut microbial biomarker combination includes *Cetobacter* spp. (…). Cetobacterium ), Pseudomonas spp. Pseudomonas ) and Escherichia coli-Shigella spp. Escherichia‑Shigella Using the intestinal microbial biomarker combination described in this application, non-invasive diagnosis of liver damage can be achieved without touching the fish body, with high accuracy and no missed detections, especially avoiding misdiagnosis as liver damage that could lead to overtreatment. This provides a basis for judgment in aquaculture environment control and precise disease prevention and control, and is beneficial for improving the survival rate and profitability of yellow catfish farming.

The invention discloses a screening culture medium and a preparation method and application thereof, the screening culture medium comprises a basic culture medium and additives, and the additives comprise amphotericin B, vancomycin, ox bile salt, sodium deoxycholate and No.3 bile salt. According to the screening culture medium provided by the invention, a special infectious microbe inhibitor is added on the basis of a trisaccharide iron culture medium, so that most gram-negative enterobacter, staphylococcus aureus and part of fungi except salmonella enteritis subspecies and shigella cannot grow; the interference of the infectious microbes on the detection of the salmonella enteritis subspecies and the shigella is avoided, so that the fluorescent probe can be applied to the detection of the salmonella enteritis subspecies and the shigella in a clinical anus swab, and the sensitivity of color development is improved.

The invention discloses an intestinal microbial marker combination and system for detecting fish liver injury and application, and belongs to the technical field of molecular detection of fish liver injury. The invention relates to an enteric microbial marker combination, which is characterized in that the enteric microbial marker combination comprises the following components: cetyl, pseudomonas and Escherichia-Shigella, and the enteric microbial marker combination comprises the following components: bacillus, pseudomonas and Escherichia-Shigella, and the enteric microbial marker combination comprises the following components: bacillus, pseudomonas and Escherichia-Shigella. By utilizing the intestinal microbial marker combination, non-invasive diagnosis of liver injury can be realized on the premise of not invading a fish body, the accuracy is high, missing detection is avoided, and particularly, the condition that liver injury is misjudged to cause excessive treatment is avoided. A judgment basis is provided for breeding environment regulation and control and precise disease prevention and treatment, the breeding survival rate of the pelteobagrus fulvidraco is increased, and the breeding benefit is improved.

The invention relates to the technical field of microorganisms, in particular to pichia ethanol yeast JSL-7 and application thereof.The pichia ethanol yeast JSL-7 is obtained by being separated from the body of a cattle, it is detected that the strain has a good antibacterial effect on escherichia coli, staphylococcus aureus and / or shigella flexneri, in addition, the strain is free of in-vivo toxicity, and the strain has a good application prospect. The strain has good cholate resistance and acid resistance, can be well colonized in intestines and stomachs of animals and has good probiotic performance, besides the probiotic performance, through analysis of metabolites, it is found that the strain can produce menthol without gene modification, which is the characteristic that a strain in the prior art does not have, and the menthol can be produced by the strain without gene modification. And after a fermentation culture medium is optimized, an optimal fermentation culture medium for preparing menthol by fermenting the strain is also obtained, so that the production time of menthol can be effectively shortened, the production process is simplified, and a new strain and a new idea are provided for producing menthol by a fermentation method in the future.

This invention discloses a quadruple Taqman qPCR reagent for detecting foodborne pathogens in aquatic products. The foodborne pathogens are Vibrio parahaemolyticus, Vibrio vulnificus, Shigella, and Staphylococcus aureus. The reagent includes specific primers and Taqman probes corresponding to the four foodborne pathogens. This invention provides a quadruple Taqman qPCR reagent with high efficiency, accuracy, sensitivity, and speed, offering an accurate, rapid, efficient, and economical method for detecting the above four foodborne pathogens in the quality and safety testing of aquatic products and their processed products. This is of great significance for ensuring the safety of aquatic products for consumption in my country.

The invention relates to the technical field of microorganisms and biological medicines, in particular to triple probiotics as well as a preparation method and application thereof, and the triple probiotics comprise at least one probiotic of bacillus, lactobacillus acidophilus and bifidobacterium longum. The triple probiotics provided by the invention form an'oxygen consumption-enzyme production-acid production 'synergistic chain, and through synergistic degradation of uric acid, inhibition of excessive growth of conditioned pathogens and enhancement of intestinal barrier functions, the blood uric acid level of patients with asymptomatic hyperuricemia is reduced, the alpha-diversity of intestinal tracts is improved, the abundance of butyric acid producing bacteria is improved, and the content of the butyric acid producing bacteria is improved. The ratio of escherichia coli to shigella is reduced; sIBO is effectively relieved or reversed, and digestive tract symptoms such as abdominal distension and exhaust are reduced. In addition, the triple probiotics avoid liver and kidney toxicity caused by chemical drugs, and are suitable for long-term administration.

The invention belongs to the technical field of biological medicine, and particularly relates to application of pseudo-ginseng carbon quantum dots in preparation of a medicine for treating renal ischemia-reperfusion injury, the carbon quantum dots are synthesized by taking pseudo-ginseng as a raw material through a hydrothermal method, and the pseudo-ginseng carbon quantum dots have the antioxidant enzyme activity property, can effectively relieve cell oxidative stress and inhibit secretion of proinflammatory cytokines, and can be used for preparing a medicine for treating renal ischemia-reperfusion injury. The pseudo-ginseng carbon quantum dots specifically gather in the damaged kidney, so that the renal ischemia reperfusion injury of the mouse is obviously relieved; the pseudo-ginseng carbon quantum dots can relieve kidney injury through the following ways: reducing abundance of escherichia coli-shigella, reducing the level of indol sulfate in the kidney, regulating tryptophan-indole metabolism, regulating an AHR-Raf-ERK signal channel, down-regulating the levels of TNF-alpha and malonaldehyde, and regulating the level of glutathione. A catalytic mechanism and a multi-omics regulatory pathway of the pseudo-ginseng carbon quantum dots in renal ischemia reperfusion injury are disclosed, and experimental evidence is provided for'intestinal tract-kidney axis' in kidney disease treatment.

A broadly protective Shigella-ETEC vaccine includes components to cover multiple Shigella species and serotypes as well as multiple different colonization factors of ETEC. ETEC colonization factors CS2 and CS3 are among the most prevalent CFs found on ETEC isolates associated with diarrhea. These two factors are important components of a vaccine that can confer broad protection against ETEC. High level expression of these heterologous antigens in Shigella live vectors results in stronger immune responses. This specification describes the genetic engineering of DNA sequences encoding upstream sequences, including promoter and ribosome binding sites, that results in increased expression of CS2 and CS3 in Shigella live vectors and that leads to stronger immune responses when used as a vaccine in animal models.

A strain of Streptomyces pseudo-light gray XH-1, its microecological preparation and its preparation method. The Streptomyces pseudo-light gray XH-1 of the present invention was deposited in the China Center for Type Culture Collection on August 26, 2024, with the strain deposit number CCTCC NO: M 20241850; the classification name is: Streptomyces pseudo-light gray XH-1, the Latin name Streptomyces pseudogriseolus XH‑1. Its microecological preparation is made by fermenting Streptomyces pseudogriseus XH‑1. The present invention also includes a method for preparing a microecological preparation of Streptomyces pseudogriseus XH‑1. Feeding fish with a fermentation liquid of an appropriate concentration of the strain can significantly improve the immune performance of the fish, especially the specific immune performance. The microecological preparation prepared by the strain also has an antibacterial effect on common fish pathogens such as Aeromonas vernix, Shigella-like Pseudomonas, and Aeromonas anomala. When Streptomyces pseudogriseus XH‑1 is added to the feed to feed fish, it is found that it can significantly increase the expression of specific immune factors and improve the disease resistance of fish.

The invention relates to the technical field of animal feed additives, in particular to a composition with bacteriostatic activity and application thereof. A screening antibacterial experiment of the plant essential oil and the organic acid shows that the composition of the plant essential oil and the organic acid, which is reasonably proportioned, has remarkable antibacterial activity on escherichia coli, salmonella typhimurium, shigella flexneri and clostridium perfringens, and particularly has a very good synergistic interaction effect. The composition of the plant essential oil and the organic acid is preferably thymol, carvacrol and sorbic acid, and the optimal compounding ratio is 3: 1: 1. The plant essential oil and organic acid composition provided by the invention has the advantages of being good in antibacterial effect, low in production cost, natural, non-toxic and the like, provides an effective treatment means for weaned piglets with bacterial diarrhea, and has huge potential commercial value.

The application discloses Slackia a new application of sp.D-G6, and particularly relates to Slackia application of sp.D-G6 bacterial powder in pig breeding, and belongs to the field of agricultural animal breeding and microbial technology. The bacterial strain has been preserved in the Guangdong Microbial Culture Collection Center on May 8, 2019, and the preservation number is GDMCC NO: 60661. Research shows that the bacterial powder has no obvious adverse effect on pigs, has good feeding safety, can promote pig feeding and weight gain, improve average daily feed intake and average daily weight gain, and improve growth performance. Further, the bacterial powder can regulate the microbial composition of the ileum of growing pigs, increase the abundance of Firmicutes and Lactobacillus, Lactobacillus and reduce the abundance of Proteobacteria and / or Escherichia-Shigella, Escherichia-Shigella and has application prospect of being developed into a new green feed additive.

The present invention provides a highly sensitive and rapid colorimetric detection method for foodborne pathogenic bacteria, comprising the following steps: preparing silver nanoparticles, gold nanoparticles and dendritic gold nanoparticles; modifying the silver nanoparticles, gold nanoparticles and dendritic gold nanoparticles with Salmonella antibodies, Staphylococcus aureus antibodies and Shigella antibodies respectively; preparing dopamine-coated modified aqueous magnetic fluid; sample detection, enriching the microorganisms in the detection sample by positive charging of the surface of magnetic nanoparticles; separating the microorganisms and matrix in the detection sample by applying an external magnetic field; using Salmonella, Staphylococcus aureus and Shigella as targets, and then detecting the pathogenic bacteria by dark field microscopy after reacting with the antibody-modified nanoparticles. The highly sensitive and rapid colorimetric detection method for foodborne pathogenic bacteria provided by the present invention does not require bacteria expansion for detection, has high detection sensitivity, and can achieve multi-target detection.