174 results about "Corynebacterium amycolatum" patented technology

L-Arginine is produced by culturing a coryneform bacterium in which an arginine repressor involved in L-arginine biosynthesis is deleted by disrupting a gene coding for the repressor, and which has L-arginine producing ability in a medium to produce and accumulate L-arginine in the medium, and collecting the L-arginine from the medium.

A method of producing coryneform bacteria having improved amino acid or nucleic acid productivity comprising the steps of introducing a mutation in a promoter sequence of amino acid- or nucleic acid-biosynthesizing genes on a chromosome of a coryneform bacterium to make it close to a consensus sequence, or introducing a change in a promoter sequence of amino acid- or nucleic acid-biosynthesizing genes on a chromosome of a coryneform bacterium by gene recombination to make it close to a consensus sequence, to obtain mutants of the coryneform amino acid- or nucleic acid-producing microorganism, culturing the mutants and selecting a mutant capable of producing the intended amino acid or nucleic acid in a large amount. This method allows the construction of a mutant capable of enriching or controlling the expression of an intended gene without using a plasmid and to promote production of amino acids in a high yield by recombination or mutation.

A plasmid which is able to be isolated from Corynebacterium thermoaminogenes, and which comprises a gene coding for a Rep protein having the amino acid sequence shown in SEQ ID NO: 4 or an amino acid sequence having homology of 90% or more to the amino acid sequence shown in SEQ ID NO: 4. and has a size of about 4.4 kb or about 6 kb, or a derivative thereof.

A colibacillus-corynebacterium inducible expression carrier pDXW-8 and a building method thereof belong to the technical field of genetic engineering. The inducible expression carrier pDXW-8 can be copied and stably exists in colibacillus and corynebacterium and comprises a tac promoter, a terminator rrnBT1T2, a resistance marker card kanamycin mark resistance gene, a negative regulation gene laclPE104 and a multi-cloning site, wherein the tac promoter plays a transcript promoting function in the colibacillus and the corynebacterium, the terminator rrnBT1T2 has a transcript stopping function, the resistance marker card kanamycin mark resistance gene is used for selecting transformants of the corynebacterium, the negative regulation gene is used for severely controlling expression of the tac promoter, and the multi-cloning site includes 11 signal limited incision enzyme sites. In the corynebacterium, the carrier is moderate in foreign protein expression, and is particularly adaptable to study on corynebacterium metabolic engineering.

The object of the present invention is to provide a method of producing a heterologous protein by making a coryneform bacterium to produce and efficiently extracellularly secrete (secreto-production) an industrially useful heterologous protein. According to the present invention, a genetic construct is used where a gene sequence encoding an intended protein which is ligated to the downstream of a sequence encoding the signal peptide derived from a coryneform bacterium, the gene construct is introduced into a mutant coryneform bacterium which has a capacity of secreting the heterologous protein at least 2-fold higher than the wild type Corynebacterium glutamicum ATCC 13869, the mutant coryneform bacterium is cultured and the extracellularly released heterologous protein is recovered.

The invention discloses a construction method and an application of a corynebacterium gene continuous knockout system, belonging to the technical field of genetic engineering. The corynebacterium gene continuous knockout system comprises a template plasmid and a temperature-sensitive expression plasmid, wherein the template plasmid provides a kan fragment (also known as a kan box) with loxp or variant loxL/R sites at two ends; and the temperature-sensitive expression plasmid carries a Cm resistance gene cat and a temperature-sensitive C. glutamicum replicon, expresses a Cre recombinant enzyme and is used for removing a Km resistance gene kan. The corynebacterium continuous knockout system disclosed by the invention can be generally used for continuous gene transformation of corynebacterium and has the advantages of simplicity and convenience in operation and high efficiency. By utilizing the knockout system, gene (continuous) knockout of three major typical subspecies genomes of the corynebacterium can be successfully completed, and the system is proved to be applicable to research and production of corynebacterium metabolites.

A composition including a purified lipid cell-wall component or analog or derivative thereof and a suitable pharmaceutical carrier, medium, excipient or adjuvant is described. The composition is useful in prophylactic and therapeutic methods of treating a microbial infection in a subject, typically a mycobacterial infection such as tuberculosis, and immune disorders, inflammatory conditions and allergies in a subject, typically autoimmune diseases. It is also useful in diagnostic methods. The purified lipid cell-wall component is typically a purified mycolic acid or a mixture of purified mycolic acids from a bacterium which produces mycolic acids. The bacterium is from Mycobacterium, Corynebacterium, Nicardia or Rhodococcus.

The invention discloses an intestinal flora maker for intestinal cancer and an application of the intestinal flora maker, and belongs to the technical fields of microorganisms and genetic engineering.The intestinal flora maker comprises megasphaera, veillenella, streptococci, bilophila sp., haemophilus, corynebacterium or Escherichia-Shigella. Through detection by the intestinal flora maker, risks of individuals suffering from intestinal cancer can be assessed, and auxiliary reference can be provided for disease diagnosis and prognosis monitoring.

An immunomodulator having suppressive activity on IgE antibody production is provided and contains bacterial cells, or their decomposition materials. It can be taken as a food. Bacterial cells such as Corynebacterium, Brevibacterium, Microbacterium, or bacterial cells of mutant strains of these bacteria, or decomposition products of these bacteria are used.

Disclosed is a method for producing 1,5-pentanediamine through fermentation using a microorganism, said microorganism having LDC gene in the chromosome and showing regulated secondary production of L-lysine. In the method for producing 1,5-pentanediamine whereby 1,5-pentanediamine is produced by a coryneform bacterium having a gene encoding lysine decarboxylase in the chromosome, said coryneform bacterium continuously maintains a lysine decarboxylase activity of 50 mU/mg protein or greater during the culture.

The invention relates to isolated nucleotide sequences from Coryneform bacteria which code for the pck gene encoding the enzyme phosphoenol pyruvate carboxykinase (PEP carboxykinase). The invention also relates a process for the fermentative preparation of L-amino acids, in particular L-lysine, L-threonine, and L-glutamate by attenuation of the pck gene.

A method of treating cancers by administering to a patient an anti-tumor effective amount ot at least one of a thermostable macromolecular antigen complex or a fragment of such a complex which is interspecific for microorganisms of the Mycobacteria, Nocardia and Corynebacteria group and which exhibits after immunoelectrophoresis an immunoelectrophoretic precipitation pattern corresponding to that of the antigen complex 60 of Mycobacteria bovis Calmette Guerin Bacillus strain. Preferably, the patient is also treated with an additional therapeutic agent which is specific against the patient's cancer such as a tumoral antigen or a non-proliferative tumor cell.

The invention relates to the field of microbial technology, in particular to a recombinant strain, a method for preparing the recombinant strain and a method for producing L-valine from the recombinant strain. According to the recombinant strain, corynebacterium serves as a starting strain for transformation, and the transformation process includes the steps of conducting point mutation on the ilvN gene and knocking out the avtA gene. The recombinant strain is obtained by conducting point mutation on the ilvN in a valine metabolism path and knocking out the avtA gene for transaminase coding; through fermentation culture, the yield of L-valine can reach 12.2 g/L, and generation of the by-product alanine is greatly reduced.

The invention provides a recombinant bacterial strain for high-yield production of L-lysine, and a construction method and application of the recombinant bacterial strain. A polynucleotide sequence comprises a modified pobB gene coding polynucleotide sequence as shown in SEQ ID NO:2, and the invention provides a recombinant protein coded by the polynucleotide sequence, the recombinant bacterial strain containing the polynucleotide sequence, a construction method of the recombinant bacterial strain, and an application of the recombinant bacterial strain in fermentation production of L-lysine. According to the recombinant strain disclosed by the invention, point mutation is carried out on the nucleotide sequence of an open reading frame coding region of the pobB gene in wild-type corynebacterium glutamicum to obtain the recombinant strain modified by the coding region of the pobB gene, and the yield of L-lysine is realized compared with the yield of L-lysine by using wild bacterial strains.

The invention relates to a corynebacterium glutamicum establishment method for increasing the yield of L-lysine and improving the stability of the L-lysine. According to the method, after a Tnp7a geneof corynebacterium glutamicum is inactivated, the corynebacterium glutamicum is established, and an amino acid sequence of the Tnp7a gene is shown in SEQ ID NO:1. According to the corynebacterium glutamicum establishment method for increasing the yield of the L-lysine and improving the stability of the L-lysine, the endogenous gene Tnp7a of the established corynebacterium glutamicum is inactivated, the transposition effect, caused when an insertion sequence is activated due to an environment change, of the corynebacterium glutamicum in the preservation and fermentation process is lowered, thestrain degeneration progress, caused by mutation or frame shift generated by transposition or adjustment on expression of adjacent genes through promoters carried by a genome, of the genome is blocked, and the yield of the L-lysine is increased.

The present invention discloses a non-cell corynebacterium parvum preparation (NCP) and its application in preparation of medicine for curing tuberculosis, in which the described NCP is a non-cell preparation prepared by breaking corynebacterium parvum, its grain size is less than 100 nm, and its heat source is less than 160 Eu/ml, protein content is 10-40% (g/g), nucleic acid content is 3-25% (g/g), the rest is polysaccharide. The tests show that it can obtain good therapeutic effect.

A DNA encoding for a mutant of LysE protein, or a homologous protein thereof, of a coryneform bacterium, wherein the mutant, when introduced into a methanol-assimilating bacterium imparts resistance to L-lysine analogue. The DNA encoding for a mutant of LysE protein, or a homologous protein thereof, is introduced into a methanol-assimilating bacterium to improve L-lysine and L-arginine productivity of the methanol-assimilating bacterium.

The present invention discloses a non-cell corynebacterium preparation (NCP). It is a non-cell preparation prepared from corynebacterium parvum (CP), its heat source is less than 160 EU/ml, protein content is 10-40 % (g/g), nucleic acid content is 3-25% (g/g), and the rest is polysaccharide, and its grain size is less than 100 nm. Said invented NCP is a nano preparation made up by breaking CP to nano-grade, and is a good immune regulating agent.