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Method for producing 1,5-pentanediamine

A manufacturing method and a technology for pentamethylenediamine, which are applied in the directions of biochemical equipment and methods, carbon-carbon lyases, enzymes, etc., can solve the problem that the fermentation yield of 1,5-pentamethylenediamine is not improved, the economic problems, and the generation of by-products. Lysine etc.

Inactive Publication Date: 2012-12-26
TORAY IND INC
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] However, there are many problems to be solved in the production method of 1,5-pentanediamine by fermentation. During cultivation, lysine is produced as a by-product (see Non-Patent Document 4)
In other words, although lysine is a precursor for the biosynthesis of 1,5-pentanediamine, there is a problem that unreacted lysine appears in a large amount in the culture supernatant
When the by-product lysine is produced as described above, although the precursor can be produced, the fermentation yield of 1,5-pentanediamine does not increase, causing economical problems

Method used

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  • Method for producing 1,5-pentanediamine
  • Method for producing 1,5-pentanediamine

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Embodiment 1、 comparative example 1

[0095] (1) Production of Corynebacterium glutamicum capable of fermenting and producing L-lysine

[0096] In order to produce Corynebacterium glutamicum capable of synthesizing L-lysine, a precursor of 1,5-pentanediamine, L-lysine-fermenting bacteria were created by introducing effective mutations into aspartokinase (AK) . Corynebacterium glutamicum AK-1 strain (hereinafter referred to as AK-1 strain) was prepared referring to the method described in Apppl. Microbiol. Biotechnol., (2002), 58, p.217-223. That is, this operation is specifically performed as follows.

[0097] Genomic DNA solution prepared according to conventional methods from Corynebacterium glutamicum ATCC13032 strain was used as an amplification template, and the oligonucleotide designed with reference to the base sequence of the AK gene (accession number: BA000036) registered in the database (GenBank) Nucleotides (sequence number: 4, 5) were used as a primer set for PCR, and the resulting product was electr...

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Abstract

Disclosed is a method for producing 1,5-pentanediamine through fermentation using a microorganism, said microorganism having LDC gene in the chromosome and showing regulated secondary production of L-lysine. In the method for producing 1,5-pentanediamine whereby 1,5-pentanediamine is produced by a coryneform bacterium having a gene encoding lysine decarboxylase in the chromosome, said coryneform bacterium continuously maintains a lysine decarboxylase activity of 50 mU / mg protein or greater during the culture.

Description

technical field [0001] The invention relates to a method for producing 1,5-pentanediamine by fermentation. Background technique [0002] Polyamide (PA) is an important polymer group used as a raw material for a series of special plastics used in the automotive industry, sports industry, and lifestyle industry, and diamine is an important raw material monomer component of the polyamide. Diamines and dicarboxylic acids condense to form various polymers, where the properties of the polymers are determined by the chain lengths of the diamines and dicarboxylic acids. [0003] In general, diamines are produced from petroleum-derived materials chemically through an intermediate stage of dicarboxylic acids; or by chemical decarboxylation of amino acids (Non-Patent Document 1). Considering the high price of petroleum, a rapid change is expected to the method of synthesizing diamines from renewable resources by biotechnological methods such as fermentation. [0004] Here, a method o...

Claims

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Application Information

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IPC IPC(8): C12P13/00C12N15/09C07C211/09C12N9/88
CPCC12P13/001C12N9/88C12N15/52C12Y401/01018C12P13/00C07C211/09
Inventor 泽井健司渡边志绪美耳塚孝泽井秀树
Owner TORAY IND INC
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