7 results about "Coryneform bacterium" patented technology

A new technique for improving the secretion production of a heterologous protein by a coryneform bacterium is developed, and thus a method for the secretion production of a heterologous protein is provided. A coryneform bacterium having a secretion production ability of a heterologous protein and modified to maintain a phoS gene having a specific mutation is cultured to produce a heterologous protein by secretion.

The application discloses a method for increasing 2-pyrrolidone synthesis and belongs to the technical field of bioengineering. tacm The CoA transferase from Butyricicoccus faecihominis is expressed in the coryneform bacterium glutamicum, and the CoA transferase is secreted and expressed by means of P The promoter and the NS signal peptide strengthen the secretory expression of the CoA transferase, so that the constructed strain can ferment 2-pyrrolidone in a glucose carbon source and in a pH 7.0 environment, and the yield of the 2-pyrrolidone can reach 12.13 g / L in 96 h of flask fermentation.

A novel technique for improving secretory production of a heterologous protein by coryneform bacteria is provided, and thereby a method for secretory production of a heterologous protein is provided. A coryneform bacterium having an ability of secretory producing a heterologous protein and having been modified so that an activity of a Mdh protein is reduced is cultured to produce the heterologous protein by secretory production.

The present invention addresses the problem of providing a method for secreting and producing a heterologous protein by developing a novel technique for improving the secreting and producing of a heterologous protein by coryneform bacteria. The present invention relates to a method for secreting and producing a heterologous protein, comprising culturing a coryneform bacterium which has the ability to secrete and produce a heterologous protein and which has been modified such that the activity of an Mdh protein is reduced, and secreting and producing the heterologous protein.

The invention relates to the technical field of microbial engineering, and discloses an L-hydroxyproline-producing de novo synthesis type corynebacterium glutamicum and a construction method thereof, and the corynebacterium glutamicum strengthens the biosynthesis flux from glucose to L-proline by overexpressing a key enzyme gene of an endogenous proline synthesis pathway; by introducing an exogenous proline-4-hydroxylase gene, the accumulated L-proline is efficiently converted into the L-hydroxyproline; meanwhile, by knocking out key genes of a competitive pathway, generation of by-products such as lysine and threonine is reduced, and metabolic interference is relieved. The invention further provides a construction method of the engineering bacterium. The construction method comprises the steps of vector construction, gene knockout and overexpression, transformation, screening and the like. According to the method, an economical and feasible foundation is laid for industrial biological manufacturing of the L-hydroxyproline, the overall conversion efficiency from a carbon source to a final product is improved, and the utilization rate of raw materials and the yield of the L-hydroxyproline are improved.

The present invention provides an artificial chromosome which can replicate in a coryneform bacterium such as Corynebacterium glutamicum, a method for editing a genome of a coryneform bacterium such as Corynebacterium glutamicum using the artificial chromosome, and a system and a kit comprising the artificial chromosome.

The present invention provides a promoter suitable for Corynebacterium stagnantum and its application. The nucleotide sequence of the promoter suitable for Corynebacterium stagnantum is shown in SEQ ID NO: 7, SEQ ID NO: 5, and / or SEQ ID NO: 6. By introducing the promoter into Corynebacterium stagnantum, the present invention achieves high-level expression of a target protein in Corynebacterium stagnantum, overcoming the problems of low strength of existing promoters, difficulty in expressing metabolites and proteins in coryneform bacteria, and low expression efficiency, thereby further improving target protein synthesis and achieving GFP protein and α-amylase expression levels 2.63 and 2.36 times higher than those of the existing Corynebacterium Psod promoter.