5 results about "Coryneform bacterium" patented technology

A new technique for improving the secretion production of a heterologous protein by a coryneform bacterium is developed, and thus a method for the secretion production of a heterologous protein is provided. A coryneform bacterium having a secretion production ability of a heterologous protein and modified to maintain a phoS gene having a specific mutation is cultured to produce a heterologous protein by secretion.

The application discloses a method for increasing 2-pyrrolidone synthesis and belongs to the technical field of bioengineering. tacm The CoA transferase from Butyricicoccus faecihominis is expressed in the coryneform bacterium glutamicum, and the CoA transferase is secreted and expressed by means of P The promoter and the NS signal peptide strengthen the secretory expression of the CoA transferase, so that the constructed strain can ferment 2-pyrrolidone in a glucose carbon source and in a pH 7.0 environment, and the yield of the 2-pyrrolidone can reach 12.13 g / L in 96 h of flask fermentation.

A novel technique for improving secretory production of a heterologous protein by coryneform bacteria is provided, and thereby a method for secretory production of a heterologous protein is provided. A coryneform bacterium having an ability of secretory producing a heterologous protein and having been modified so that an activity of a Mdh protein is reduced is cultured to produce the heterologous protein by secretory production.

The invention relates to the technical field of microbial engineering, and discloses an L-hydroxyproline-producing de novo synthesis type corynebacterium glutamicum and a construction method thereof, and the corynebacterium glutamicum strengthens the biosynthesis flux from glucose to L-proline by overexpressing a key enzyme gene of an endogenous proline synthesis pathway; by introducing an exogenous proline-4-hydroxylase gene, the accumulated L-proline is efficiently converted into the L-hydroxyproline; meanwhile, by knocking out key genes of a competitive pathway, generation of by-products such as lysine and threonine is reduced, and metabolic interference is relieved. The invention further provides a construction method of the engineering bacterium. The construction method comprises the steps of vector construction, gene knockout and overexpression, transformation, screening and the like. According to the method, an economical and feasible foundation is laid for industrial biological manufacturing of the L-hydroxyproline, the overall conversion efficiency from a carbon source to a final product is improved, and the utilization rate of raw materials and the yield of the L-hydroxyproline are improved.

The present invention provides an artificial chromosome which can replicate in a coryneform bacterium such as Corynebacterium glutamicum, a method for editing a genome of a coryneform bacterium such as Corynebacterium glutamicum using the artificial chromosome, and a system and a kit comprising the artificial chromosome.