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Method for manufacturing L-amino acid

An amino acid and serine technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve problems such as unknown activity associations

Active Publication Date: 2015-10-28
AJINOMOTO CO INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the link between the activity of these phosphate transporters and the production of L-amino acids is unknown

Method used

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  • Method for manufacturing L-amino acid
  • Method for manufacturing L-amino acid
  • Method for manufacturing L-amino acid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0303] Example 1: Glu production culture using a pitA-enhanced strain

[0304] In this example, Glu production was performed using a Glu-producing strain of Corynebacterium glutamicum in which expression of the pitA gene was enhanced, and the effect of enhanced expression of the pitA gene on Glu production was evaluated.

[0305] The strains used are described below.

[0306] Corynebacterium glutamicum 2256ΔldhAΔsucA yggB * / pVK9

[0307] Corynebacterium glutamicum 2256ΔldhAΔsucA yggB * / pVK9-Plac-pitA

[0308] (1) Strain construction method

[0309] Using Corynebacterium glutamicum 2256 strain (ATCC 13869) as a parent strain, 2256ΔldhAΔsucA yggB was constructed by the following method * strains served as model Glu-producing strains. The primers used are shown in Table 1.

[0310] Table 1

[0311]

[0312] First, using the chromosomal DNA of the 2256 strain as a template, a pair of primers 1 and 2, and a pair of primers 3 and 4 were used to amplify the DNA fragment ...

Embodiment 2

[0327] Example 2: Glu production culture using pitA mutant strain

[0328] In this example, Glu production was performed using a Glu-producing strain of Corynebacterium glutamicum in which a mutation was introduced into the pitA gene, and the effect of the mutation of the pitA gene on Glu production was evaluated.

[0329] The strains used are described below.

[0330] Corynebacterium glutamicum 2256ΔldhAΔsucA yggB *

[0331] Corynebacterium glutamicum 2256ΔldhAΔsucA yggB * pit Amut

[0332] (1) Strain construction method

[0333] The model Glu production strain constructed in Example 1, that is, Corynebacterium glutamicum 2256ΔldhAΔsucA yggB * As a parent strain, construct 2256ΔldhAΔsucA yggB with a mutation introduced into the pitA gene * pitAmut strain. The primers used are shown in Table 4.

[0334] Table 4

[0335]

[0336] The plasmid pBS4S-pitAmut for introducing the itA mutation was constructed by the following method. Using the chromosomal DNA of the Glu-...

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Abstract

A method for producing an L-amino acid is provided. An L-amino acid is produced by culturing a coryneform bacterium having an L-amino acid-producing ability, which has been modified so that the activity of a phosphate transporter is increased, in a medium, and collecting the L-amino acid from the medium.

Description

technical field [0001] The present invention relates to a method for producing L-amino acid using coryneform bacteria. L-amino acid is industrially useful as an additive for animal feed, a seasoning or a component of food and drink, or an amino acid infusion solution. Background technique [0002] L-amino acid can be industrially produced, for example, by a fermentation method using various microorganisms capable of producing L-amino acid. Examples of methods for producing L-amino acids by fermentation include methods using wild-type microorganisms (wild strains), methods using auxotrophic strains derived from wild strains, and methods using various chemical antibiotics derived from wild strains. A method of metabolically regulating mutant strains, a method of using a strain having properties of both an auxotrophic strain and a metabolically regulating mutant strain. [0003] In addition, in recent years, microorganisms whose L-amino acid production capacity has been enhan...

Claims

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Application Information

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IPC IPC(8): C12N15/09C12N1/21C12P13/04C12P13/14C12R1/15
CPCC12P13/14C07K14/34C12P13/04
Inventor 平野圣子林和之
Owner AJINOMOTO CO INC
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