11702results about "Peptides" patented technology

Compounds or their pharmaceutically acceptable salts can modulate the G12D mutant of Kirsten rat sarcoma (KRAS) protein and are expected to have utility as therapeutic agents, for example, for treating cancer. The disclosure also provides pharmaceutical compositions which comprise compounds disclosed herein or pharmaceutically acceptable salts thereof. The disclosure also relates to methods for use of the compounds or their pharmaceutically acceptable salts in the therapy and prophylaxis of cancer and for preparing pharmaceuticals for this purpose.

The invention relates to the technical field of antibacterial peptides, and particularly discloses a marine antibacterial peptide and application thereof, and the amino acid sequence of the marine antibacterial peptide is as shown in SEQ ID NO.1: MWKLPKWRNGRRRGFKIPRW. The marine antibacterial peptide CD109 provided by the invention has an inhibition effect on staphylococcus aureus, acinetobacter baumannii, pseudomonas aeruginosa and klebsiella pneumoniae, and has extremely low cytotoxicity.

Described herein, inter alia, are GTPase inhibitors and uses thereof.

Provided is a medicine for treating or preventing cancer that contains a first active ingredient and that is used in combination with a second active ingredient, wherein: the first active ingredient is a compound represented by formula (1), a pharmaceutically acceptable salt thereof, or a pharmaceutically acceptable solvate thereof; and the second active ingredient is GDC-6036 or a pharmaceutically acceptable salt thereof.

This disclosure provides cyclic peptide compounds of formula (A) or formula (II) as inhibitors of Ras kinase, and compositions and use of the same in treating diseases associated with Ras kinase.

Systems for plant culture include a chamber featuring one or more walls enclosing a spatial volume internal to the chamber, where the one or more walls include a surface for supporting a plant within the enclosed spatial volume, a gas delivery apparatus with at least one gas source, a nutrient delivery apparatus with a reservoir, a sampling apparatus connected to a port formed in the one or more walls, and a controller configured so that during operation of the system, the controller activates the nutrient delivery apparatus to deliver an aqueous growth medium to the plant, and activates the gas delivery apparatus to deliver into the enclosed spatial volume a mixture of isotopically-substituted gases. Also provided are methods of use of the system for measuring nitrogen in a plant and for identifying microbes capable of providing fixed nitrogen to a plant.

The invention belongs to the technical field of genetic engineering and metabolic engineering, and discloses an escherichia coli genetically engineered bacterium for fermentation production of L-histidine, a method and application of the escherichia coli genetically engineered bacterium for fermentation production of L-histidine. The escherichia coli genetically engineered bacterium is prepared by taking wild escherichia coli MG1655 as a starting strain, and knocking out a gene lacI and a gene purR on a genome; integrating the gene prs, the gene zwf and the gene gnd; the method comprises the following steps: integrating genes hisG, hisD, hisB, hisC, hisA, hisF / H and hisIE; and a gene cgl2458 is integrated. According to the invention, a molecular biological technology is utilized to integrate histidine operon in a serratia marcescens mutant strain with high histidine yield into an escherichia coli genome, and an escherichia coli genetically engineered strain with high histidine yield is obtained by enhancing precursor PRPP supply, histidine efflux and other strategies, so that the problems of thick fermentation liquor, high histidine yield and high histidine yield can be solved. And the method has a relatively good industrial application potential.

The invention belongs to the technical application field of microbial metabolic engineering and synthetic biology, and particularly relates to a pichia pastoris engineering strain for high-yield sclareol as well as a construction method and application of the pichia pastoris engineering strain. The method comprises the following steps: constructing a biosynthetic pathway of sclareol in a host strain, and optimizing an intracellular mevalonic acid metabolic pathway and a central metabolic pathway to obtain an engineering strain A; the host strain is pichia pastoris; or overexpressing or knocking out a metabolism regulation factor in the obtained engineering bacterium A to obtain an engineering bacterium strain B; or, in the obtained engineering bacterium B, a synthetic pathway is targeted to peroxisome and optimized by adopting a cell region compartment strategy, and an engineering bacterium strain C is obtained. The invention provides a pichia pastoris chassis cell for synthesizing a diterpenoid sclareol and an engineering strain for synthesizing sclareol. By introducing and optimizing the expression of a sclareol synthesis pathway, the fermentation yields of sclareol in shake flask batch and bioreactor batch fed-batch fermentation are respectively 631.6 mg / L and 10.5 g / L.

The invention relates to a fluorescent protein selection marker staygold-levEr gene as well as a construction method and application thereof, and belongs to the technical field of biological engineering. The invention provides a fluorescent protein selection marker staygold-levEr gene for screening an L-valine production strain and a construction method of a gene fragment, compared with other similar gene selection markers, the selection marker provided by the invention has the advantages that the fluorescence intensity is positively correlated with the concentration of L-valine, and the screening marker has the advantages that the screening marker can be used for screening the L-valine production strain; according to the screening marker, the screening efficiency of the L-valine high-yield strain can be obviously improved, the screening time and cost can be saved, the screening efficiency can be improved, and the screening marker has a wide application prospect.

The invention discloses an antibacterial peptide and antibacterial application thereof. The amino acid sequence of the antibacterial peptide is as shown in SEQ ID No.1, and the C end of the antibacterial peptide is amidated. The antibacterial peptide provided by the invention has the activity of resisting staphylococcus aureus and escherichia coli, and plays a role in inhibiting bacteria by destroying cell membranes. The antibacterial peptide is excellent in antibacterial activity, good in stability and not prone to generating drug resistance, can be used for synthesizing and preparing antibacterial compositions, and has great potential in application in the fields of food and medicine.

Disclosed are a hexapeptide compound and a composition and use thereof, relating to the technical field of polypeptides, the peptide or a salt thereof having a structure as shown in formula (I): R1-Val-Leu-Gln-Trp-Val-Lys-R2 (I). In particular, the invention relates to the peptide or the salt thereof, or a composition of the peptide and the salt thereof, and application of the peptide and the salt in preparation of a composition for nursing or treating skin or mucous membranes.

The invention relates to the field of preparation and application of biological peptides, in particular to a hippocampal swelling peptide and application thereof in preparation of functional products for repairing male reproductive system injury. A green controllable enzymolysis technology is utilized to obtain protein polypeptide with nutritional, health-care and medicinal values, an active peptide sequence with high biological activity is obtained through further research on the basis, high-purity active peptide is obtained through a chemical synthesis mode, and the biological activity of the peptide is verified through mouse spermatogonium.

The invention discloses acid-resistant pichia kudriavzevii and an application of the acid-resistant pichia kudriavzevii. The acid-resistant pichia kudriavzevii is Pichia kudriavzevii S9 and is preserved in the Guangdong Microbial Culture Collection Center on September 20, 2024, and the preservation number of the acid-resistant pichia kudriavzevii S9 is GDMCC.No: 65148. The acid-resistant pichia kudriavzevii S9 has the advantages that the acid-resistant pichia kudriavzevii S9 is used for According to the invention, a low-pH-resistant pichia kudriavzevii strain is screened from surface microorganisms of grapes, and the pichia kudriavzevii strain can tolerate an environment with pH of 1.8 and ferment organic acid under the condition of not adding a neutralizing agent; besides, genetic modification is carried out based on pichia kudriavzevii, including knocking out succinic acid degradation related genes, knocking out ethanol and glycerol production pathways and enhancing succinic acid transporter expression, so that engineering bacteria for high-yield succinic acid are obtained, and the fermentation yield of succinic acid reaches 31.74 g / L under the condition that a neutralizer is not added.

The invention belongs to the technical field of biochemical engineering, and provides a method for increasing L-carnosine yield of escherichia coli by increasing PRPP supply, and the yield and yield of L-carnosine are remarkably increased by up-regulating expression of a prs gene, increasing PRPP content in cells and promoting synthesis of L-histidine. Experiments show that the yield of the L-carnosine in different recombinant strains is increased by 55.56%-140%, and L-histidine and beta-alanine do not need to be added in the production process.

The invention discloses a xanthine oxidase inhibitory peptide with a kidney improving function and application thereof, and belongs to the technical field of biological medicines.The xanthine oxidase inhibitory peptide is screened out by conducting enzymolysis, separation and purification on litopenaeus vannamei processing by-products and utilizing a molecular docking technology, the amino acid sequence of the xanthine oxidase inhibitory peptide is Pro-Leu-Gly-Pro-Pro-Pro, and the amino acid sequence of the xanthine oxidase inhibitory peptide is Pro-Leu-Gly-Pro-Pro-Pro. And the half inhibition concentration of the polypeptide is 2.356 + / -0.2448 mM. The xanthine oxidase inhibitory peptide is proved to have an improvement effect on hyperuricemia mice, the improvement effect is mainly characterized by reducing the content of serum uric acid, creatinine and urea nitrogen of the hyperuricemia mice, inhibiting the activity of xanthine oxidase (XOD) in serum and livers of the mice, inhibiting synthesis of uric acid and improving kidney injury of the mice, and Toxinpred prediction shows that the xanthine oxidase inhibitory peptide has the effect of inhibiting the activity of xanthine oxidase (XOD) in the serum and livers of the mice. The xanthine oxidase inhibitory peptide is free of toxic and side effects, so that the xanthine oxidase inhibitory peptide has a wide application prospect in preparation of food or drugs for improving renal functions.

The invention relates to a method for screening and preparing a horse milk whey protein source antioxidant peptide and application of the horse milk whey protein source antioxidant peptide. The polypeptide with potential antioxidant activity is screened out by predicting information of the polypeptide through virtual enzymolysis and a computer and then performing molecular docking with the keap1 protein. The feasibility of obtaining the antioxidant peptide is verified by simulating gastrointestinal digestion with pepsin, trypsin and chymotrypsin. Finally, in-vitro chemical antioxidant activity verification is carried out through chemical synthesis of the high-purity antioxidant peptide, and it is found that the antioxidant peptide has good free radical scavenging activity. An oxidative damage model is induced by H2O2 on cells, and it is found that the antioxidant peptide can resist cell oxidative damage by exciting the activity of a series of antioxidant enzymes. The natural antioxidant peptide screened by the invention has wide application prospects in the fields of foods, medicines, cosmetics and the like, and an efficient way is provided for development of horse milk whey protein source functional polypeptide products.

The invention relates to an engineering bacterium of high-yield ornamental blue as well as a construction method and application of the engineering bacterium, and belongs to the technical field of natural dye synthesis by a biological method. The engineering bacterium capable of highly producing the ornamental blue, disclosed by the invention, expresses icd, bpsA, glnAY405F and gd hA; acnR, yggB, glsK, aceA and ldh are knocked out from the engineering bacteria. According to the application, yggB and aceA genes are knocked out, site-directed mutagenesis (Y405F) is carried out on a glnA gene, a glsK gene is replaced by a glnAY405F gene, an acnR gene is replaced by an icd gene, an ldh gene is replaced by a gdhA gene, and a bpsA gene is integrated, so that the formation of by-products such as lactate and succinic acid in a blue-viewing synthesis process can be blocked; the flow direction of a passage from citric acid to isocitric acid to alpha-ketoglutaric acid to glutamic acid to glutamine is improved, so that the concentration of glutamine in cells is improved, and the yield of the ornamental blue is improved.

The invention relates to the technical field of antibacterial peptide application, in particular to an antibacterial peptide for inhibiting drug-resistant acinetobacter baumannii as well as a preparation method and application of the antibacterial peptide. The antibacterial peptide is cd77AHP-2, and the amino acid sequence of the antibacterial peptide is shown as SEQ ID NO.1. The antibacterial peptide cd77 obtained through artificial chemical synthesis is modified, distribution of cationic amino acid is optimized, and the antibacterial activity of the antibacterial peptide is remarkably improved. The antibacterial peptide cd77AHP-2 has a remarkable antibacterial effect on acinetobacter baumannii which are gram-negative bacteria and multi-drug resistant acinetobacter baumannii, and the antibacterial peptide cd77AHP-2 has a remarkable antibacterial effect on the acinetobacter baumannii which are gram-negative bacteria. The antibacterial peptide cd77AHP-2 provided by the invention has relatively low cytotoxicity to human bronchial epithelial cells, and has the potential of preparing a drug for resisting drug-resistant acinetobacter baumannii. The invention provides a novel functional polypeptide for the field of antibacterial peptides, and the novel functional polypeptide has important practical application value.

The invention discloses a streptomycete recombinant engineering strain for producing mycosporine-like metabolites, a gene expression cassette is integrated in a genome of the streptomycete recombinant engineering strain, the gene expression cassette is recombined to an attB site of a chromosome in a streptomycete chassis strain, the gene expression cassette is an operon controlled by a single promoter, and the operon is an operon controlled by a single promoter. The operon comprises 2-4 biosynthetic gene elements in MysA, MysB, MysC and MysD or mysDp334 in a synthetic pathway of mycosporine-like metabolites. The invention also discloses a construction method of the streptomyces recombinant engineering strain and an application of the streptomyces recombinant engineering strain in preparation of the mycosporine-like metabolites. A mycosporine-like metabolite is efficiently produced by heterologous expression of a mycosporine-like metabolite synthesis gene element in streptomyces, and four mycosporine-like metabolite products with high yield and strong ultraviolet absorption activity can be obtained by reconstruction of the biosynthesis gene element and recombinant expression in streptomyces pluvialis.

The invention discloses recombinant escherichia coli for inactivating or weakly expressing SPMB family inner membrane protein and application of the recombinant escherichia coli in production of L-amino acid. The invention relates to the technical field of gene engineering, and provides recombinant escherichia coli, and SPMB family inner membrane protein in the recombinant escherichia coli is inactivated or reduced in activity. According to the invention, the yjiG gene (coding gene of SPMB family inner membrane protein) in escherichia coli is knocked out, so that the expression intensity of the yjiG gene is weakened, and fermentation production strains of L-threonine, L-tryptophan, L-arginine and L-valine, which are more cost-effective, can be produced. The invention has important significance for improving the fermentation yield of bacteria, namely L-threonine, L-tryptophan, L-arginine and L-valine.

The invention discloses a sea cucumber protein heptapeptide with functions of reducing blood pressure and blood sugar and application of the sea cucumber protein heptapeptide, and belongs to the technical field of bioactive peptide biology. The amino acid sequence of the sea cucumber protein heptapeptide is LPPGPFP. The sea cucumber protein heptapeptide can significantly inhibit the activity of ACE through a competitive inhibition mode, tolerates the gastrointestinal tract digestion environment, also has DPP-IV inhibition activity, can relieve hypertension and type 2 diabetes mellitus at the same time, and has an application value in preparation of products with the efficacy of reducing blood pressure and blood sugar due to multiple functions of the sea cucumber protein heptapeptide. The sea cucumber protein heptapeptide provided by the invention can significantly inhibit the activity of ACE through a competitive inhibition mode, has DPP-IV inhibition activity, has the characteristics of simple structure, safety, strong activity and the like, and has a wide application prospect.

The invention provides an antibacterial peptide cAMP048 sourced from microorganisms in a deep sea environment and application of the antibacterial peptide cAMP048, and belongs to the technical field of peptide inhibitors. The antibacterial peptide cAMP048 has an amino acid sequence as shown in SEQ ID NO: 1 (Sequence Identity Number 1). The antibacterial peptide can inhibit the growth of acinetobacter baumannii at 64 mu M, inhibit the growth of multi-drug-resistant acinetobacter baumannii and multi-drug-resistant enterococcus faecium at 128 mu M and inhibit the growth of escherichia coli, staphylococcus aureus and multi-drug-resistant klebsiella pneumoniae at 256 mu M. The antibacterial peptide can be used for preparing products with antibacterial and / or bactericidal performance, and can be applied to preparation of antibacterial and / or bactericidal products. Such as food, skin care products or medical supplies.

The invention discloses hydrolyzed egg yolk powder rich in growth factor-like polypeptide and a preparation method and application thereof, relates to the technical field of biology and the field of nutrition and health, and particularly relates to the hydrolyzed egg yolk powder rich in growth factor-like polypeptide and application of the hydrolyzed egg yolk powder as a medicament for promoting bone growth. The hydrolyzed egg yolk powder is extracted from egg yolk through a specific enzymolysis technology, and is rich in growth factor-like polypeptides with a remarkable osteogenesis promoting function. The hydrolyzed egg yolk powder and the growth factor-like polypeptide can be used as food additives or medicine components, can effectively promote bone growth, and have remarkable effects on treating or preventing bone health related diseases such as osteogenesis imperfecta, osteoporosis and arthrosis deformans.

The invention relates to the technical field of genetic engineering, and particularly discloses a genetic engineering bacterium for producing melanin as well as a construction method and application of the genetic engineering bacterium. According to the application, the yield of the melanin can be effectively increased by modifying a metabolic pathway of the genetically engineered bacterium, and the yield of the melanin reaches 6.2 g / L and 18.5 g / L respectively through shake-flask culture and 5L fermentation tank culture. Compared with the prior art, the yield of melanin produced by the genetically engineered bacterium is improved by 30% at the shake flask fermentation level, the fermentation yield of a fermentation tank is improved by 4.3 times, in addition, expensive tyrosine is changed into glucose as a fermentation precursor substance, the production cost is greatly reduced, and an efficient and feasible solution is provided for large-scale production of melanin.

The invention discloses a method for improving beef quality, an action mechanism and an experimental method, in a cattle body, vitamin A can activate the expression of EBF2 through an active metabolite RA of the vitamin A, and the EBF2 can inhibit the transcription process of CYP26B1 in a targeted manner to maintain the activity of a retinol signal channel, so that PPAR gamma and downstream lipid metabolism related genes thereof are activated, and the activity of the retinol signal channel is improved. Fatty acid transport and lipid accumulation in fat cells in the cattle muscle are promoted, fat deposition in the cattle muscle is promoted, and the beef quality is improved. By constructing the molecular network for regulating and controlling the formation of the fat in the cattle muscle, a complex regulation and control mechanism for controlling the fat deposition in the cattle muscle can be understood more deeply, a method for improving the beef quality based on regulating and controlling the vitamin A mediated key factor EBF2 is provided, and a new way is provided for improving the beef quality in a targeted manner.

The invention discloses a bran coat polypeptide GR-12 with an immunoregulation function and an application of the bran coat polypeptide GR-12, and the amino acid sequence of the bran coat polypeptide is Gly-Gln-Pro-Trp-Pro-Pro-Ala-Ser-Phe-Ala-Cys-Arg (GQPWPPASFACR, which is called GR-12 for short). Experiments find that the GR-12 can significantly enhance the proliferation activity and phagocytic activity of macrophages RAW264.7 and promote the macrophages RAW264.7 to secrete cytokines; meanwhile, it is found that the GR-12 can significantly enhance the immunomodulatory function of an immunosuppressive mouse induced by cyclophosphamide, specifically, the body weight, thymus index and spleen index of the immunosuppressive mouse are significantly recovered; and the secretion of cytokines and immune globulins in mouse serum is obviously increased. The bran coat polypeptide GR-12 is expected to be further researched and developed into a related medicine product with an immune regulation function, and has a wide application prospect.

The invention discloses an antibacterial peptide SAMP4 or a derivative thereof, a pharmaceutical composition and application, and belongs to the technical field of biology, the amino acid sequence of the antibacterial peptide SAMP4 is as shown in SEQ ID NO: 1, and the antibacterial peptide SAMP4 has a broad-spectrum antibacterial effect and can be used for preparing antibacterial peptide SAMP4. The compound has a strong bacteriostatic effect on various gram-positive bacteria such as staphylococcus aureus, enterococcus and streptococcus and various gram-negative bacteria such as escherichia coli, acinetobacter baumannii and pseudomonas aeruginosa, and is strong in antibacterial activity, wide in antibacterial spectrum and low in toxicity. The invention also provides a derivative and a salt of the antibacterial peptide SAMP4 and application of the antibacterial peptide SAMP4 and the derivative and the salt of the antibacterial peptide SAMP4 to research, development and preparation of various antibacterial products or / and antibacterial drugs and treatment of bacterial infectious diseases, and the antibacterial peptide SAMP4 and the derivative and the salt of the antibacterial peptide SAMP4 are remarkable in advantages and wide in application prospect.

The invention discloses a walnut meal-derived antibacterial polypeptide and application thereof, and belongs to the technical field of biology. The amino acid sequence of the walnut meal-derived antibacterial polypeptide disclosed by the invention is shown as any one of SEQ ID NO.1-3. The walnut meal is adopted as the raw material, the antibacterial polypeptide is extracted and separated, the utilization rate of the walnut meal can be effectively increased, resource waste is reduced, a new polypeptide variety is provided for the antibacterial polypeptide field, and new resources are provided for development of novel antibacterial agents, bacterial lysis solutions, food preservatives and anti-infection drugs.

The invention relates to the technical field of antibacterial peptide application, in particular to an antibacterial peptide for inhibiting drug-resistant staphylococcus aureus and application of the antibacterial peptide. The antibacterial peptide is obtained by performing N-terminal acetylation modification and C-terminal amidation modification on polypeptide as shown in SEQ ID NO.1. The invention further discloses a preparation method of the antibacterial peptide. The antibacterial peptide provided by the invention is a novel cationic antibacterial peptide, the minimum inhibitory concentrations of the antibacterial peptide to pathogenic bacteria staphylococcus aureus ATCC12600 and staphylococcus aureus ATCC33591 are respectively 4 [mu] g / mL and 32 [mu] g / mL, the biological safety is extremely high, and due to the characteristics, the antibacterial peptide shows extremely high medical drug antibacterial value, and can be used for preparing the antibacterial peptide. Alternative medicinal resources are provided for clinically resisting the two human pathogenic bacteria in the future.

The invention discloses an active polypeptide Tigerin-OL1 capable of promoting healing of oral ulcer and application of the active polypeptide Tigerin-OL1. The active polypeptide Tigerin-OL1 capable of promoting healing of the oral ulcer has an amino acid sequence ICTMIPISRCY as shown in SEQ ID No.1 (sequence identifier number 1). The application of the polypeptide Tigerin-OL1 is the application of the polypeptide Tigerin-OL1 in preparation of drugs for promoting healing of oral ulcer. The polypeptide Tigerin-OL1 provided by the invention has excellent oral ulcer healing promoting activity in vivo and in vitro, only contains 11 amino acids and is easy to synthesize, and a new way is provided for development of drugs for promoting oral ulcer healing.