The present invention relates to a method for separating and preparing
milk fat globule
membrane protein from buttermilk, a by-product of butter, comprising: controlling the temperature of the buttermilk, a by-product of butter, at 25 to 30 °C; adjusting the pH value to 4.5 to 4.7 and centrifuging; performing thermal
calcium treatment and centrifuging; performing ultrasonic treatment and then cooling to
room temperature; performing
dialysis treatment; adjusting the pH value to 6-8, and then performing
ultrafiltration treatment, and collecting the solution obtained by the
ultrafiltration treatment as the
milk fat globule
membrane protein. The method of the present invention can increase the removal rate of
casein to 95%, avoid the effective
protein being dialyzed or filtered out, greatly reduce the
loss rate of the
milk fat globule
membrane protein, make the extraction rate reach more than 70%, and the purity of the prepared
milk fat globule membrane reaches 87.7%. Moreover, the
raw material selected in the method of the present invention is buttermilk, a by-product of industrial butter production, which is usually discarded or made into
animal feed. The present invention reasonably utilizes this
raw material, which can not only improve the
utilization rate of buttermilk, but also save costs.