154 results about "Enterococcus faecium" patented technology

The invention provides an antibacterial peptide cAMP048 sourced from microorganisms in a deep sea environment and application of the antibacterial peptide cAMP048, and belongs to the technical field of peptide inhibitors. The antibacterial peptide cAMP048 has an amino acid sequence as shown in SEQ ID NO: 1 (Sequence Identity Number 1). The antibacterial peptide can inhibit the growth of acinetobacter baumannii at 64 mu M, inhibit the growth of multi-drug-resistant acinetobacter baumannii and multi-drug-resistant enterococcus faecium at 128 mu M and inhibit the growth of escherichia coli, staphylococcus aureus and multi-drug-resistant klebsiella pneumoniae at 256 mu M. The antibacterial peptide can be used for preparing products with antibacterial and / or bactericidal performance, and can be applied to preparation of antibacterial and / or bactericidal products. Such as food, skin care products or medical supplies.

The invention discloses a compound micro-ecological preparation for aquatic products as well as a preparation method and application thereof, and belongs to the technical field of micro-ecological preparations. The microecological preparation disclosed by the invention comprises clostridium butyricum, bacillus licheniformis, enterococcus faecalis and lactobacillus plantarum. The microecological preparation is combined with concentrated cottonseed protein for use, can replace fish meal, is added into feed, can remarkably improve the immunocompetence, oxidation resistance and digestive enzyme activity of animals, and can improve the relative abundance of beneficial bacteria in intestinal tracts, inhibit the growth of pathogenic bacteria and regulate the microecological balance of the intestinal tracts after entering the intestinal tracts of the animals. According to the invention, aerobic bacteria and anaerobic bacteria are cooperatively used, oxygen in the surrounding environment is greatly consumed by bacillus licheniformis and enterococcus faecalis, the proliferation of beneficial bacteria such as clostridium butyricum and lactobacillus plantarum is promoted, and the probiotic effect is fully played.

The invention discloses a compound microbial agent for preventing and treating fusarium wilt of cotton and application of the compound microbial agent, and belongs to the technical field of biological prevention and treatment of plant diseases. The prepared compound microbial agent is obtained by mixed culture of clostridium butyricum JBH-BD1 fermentation centrifugate, enterococcus faecium JBHBIO-BSC1, glucose and bacillus subtilis JBH-BK8, the preparation method is simple, and industrialized production, popularization and application are facilitated. The compound microbial agent has an obvious inhibition effect on cotton fusarium wilt caused by fusarium oxysporum, and the bacteriostasis rate reaches 66.7%. The harm of the cotton fusarium wilt to cotton is effectively reduced by utilizing a biotechnology, meanwhile, compared with a chemical agent, the problems of environmental pollution and the like are effectively avoided, and the application prospect is very wide.

Compositions comprising 5. salivarius strain SALI-10 and / or salivarius 10 peptide are taught. Also taught are methods of treating bacterial infections comprising administering a pharmaceutically acceptable amount of the composition to the patient with a bacterial infection caused by a pathogen from, for example, multi-drug resistant (MDR) Streptococcus pneumoniae, Streptococcus pyogenes, Streptococcus dysagalactiae, vancomycin-resistant Enterococcus faecium, Porphyromonas gingivalis, Tannerella forsythia, and MDR Enterococcus faecalis.

The invention discloses an enterococcus faecalis bacteriophage and application thereof, and relates to the field of bioengineering, on the basis that E. faecalis YN772 is obtained through separation, E. faecalis YN772 is taken as host bacteria to be co-cultured with stomatological sewage which is not subjected to chlorine treatment, enterococcus faecalis lytic bacteriophage PEf772 is separated, the bacteriophage is preserved in China Center for Type Culture Collection (CCTCC), the preservation number is CCTCC NO: M 20241844, the preservation date is August 26, 2024, the preservation number is CCTCC NO: M 20241844, and the preservation number is CCTCC NO: M 20241844. According to the research on physiological characteristics, the phage PEf772 belongs to a tail phage and a myelocaudaceae phage, the infection temperature range is 20-42 DEG C, the optimum temperature is 37 DEG C, and the phage PEf772 has high-temperature tolerance and has activity when the pH value is 3-12. In addition, the bacteriophage PEf772 not only can efficiently and continuously remove an E.faecalis YN772 biological membrane, but also can effectively inhibit the growth of the E.faecalis YN772, and a new thought and a potential treatment strategy are provided for clinically treating the refractory periapical periodontitis caused by enterococcus faecalis.

The invention relates to the technical field of microorganisms, in particular to enterococcus faecalis capable of degrading uric acid and application of the enterococcus faecalis, the enterococcus faecalis is named as Enterococcus faecalis SX3, and the preservation number of the enterococcus faecalis is CCTCC NO: M 2025248. The enterococcus faecalis has the advantages of being wide in antibacterial spectrum, rapid in growth, high in stress resistance, high in biological safety and the like, and important application value is provided for formulating a scheme for degrading uric acid.

The invention discloses a stichopus japonicus fry cultivation method, and belongs to the technical field of stichopus japonicus fry cultivation. Comprising the following steps: splashing a fermented feed containing a bacillus subtilis fermentation liquid and a bacterial liquid containing an enterococcus faecalis fermentation liquid and a lactobacillus casei fermentation liquid into a culture pond of which the pond bottom is paved with a polyethylene corrugated plate substratum; the fermented feed and the bacterial liquid are continuously splashed until the water quality in the culture pond is clear and transparent, the feed on the corrugated plate is fluffy and is in a short villus shape, and a stable beneficial bacterium phase is formed; peeling off the juvenile sea cucumbers on the 40th day after metamorphosis and attachment, distributing the juvenile sea cucumbers into a cultivation pond for cultivation, and feeding fermented feed and bacterial liquid every day. The method is applied to the aspect of stichopus japonicus fry cultivation, the problems that in existing stichopus japonicus fry production, diseases occur frequently, most fry breeding fields face poor fry quality, and the yield is low are solved, a stable cultivation environment dominated by beneficial bacteria is built, external multi-dimensional disease sources are buffered, blocked and eliminated continuously, and the survival rate of the stichopus japonicus fry is increased. The stichopus japonicus seedlings can grow in the environment without stress.

The invention provides a primer group, a kit and a method for simultaneously detecting 11 microorganisms and diagnosing chronic endometritis intrauterine infection. The primer group is used for detecting the existence of 11 chronic endometritis pathogens: chlamydia trachomatis, enterococcus faecalis, escherichia coli, trichomonas vaginalis, klebsiella pneumoniae, mycoplasma hominis, neisseria gonorrhoeae, staphylococcus, streptococcus, reproductive mycoplasma and ureaplasma urealyticum. The primer group is used for performing multiple joint detection on chronic endometritis related pathogens, has high specificity and sensitivity, and is adaptive to an endometrial tissue sample. The invention provides a method for simultaneously detecting 11 microorganisms for diagnosing chronic endometritis intrauterine infection, which comprises the following steps of: collecting an endometrial sample by adopting a disposable sterile endometrial sampler, detecting 11 microorganisms in the endometrial sample by adopting a multiplex PCR (Polymerase Chain Reaction) technology, and accurately detecting the intrauterine microbial infection condition. Meanwhile, more accurate technical support is provided for clinically identifying whether pathogenic bacteria causing chronic endometritis exist or not, and clinical doctors can comprehensively consider medication guidance conveniently.

Provided is a growth inhibitor for Clostridioides difficile. A growth inhibitor for Clostridioides difficile, comprising killed Enterococcus faecalis. Also provided are a parenteral pharmaceutical preparation, a bactericide, a disinfectant, an antibacterial agent, a sanitizer or a detergent for inhibiting the growth of Clostridioides difficile.

According to the invention, the traditional fermented koumiss in Xinjiang is collected, two novel multifunctional probiotic strains, namely, the lactobacillus plantarum SR6 GDMCC NO.66817 strain and the enterococcus faecalis SM5 GDMCC NO.66816 strain with good compatibility are separated and screened out, and the two strains have the characteristics of reducing blood fat, reducing cholesterol, removing triglyceride, resisting oxidation and antagonizing pathogenic bacteria, and have the characteristics of good cholate and gastrointestinal tract tolerance, high survival rate and good safety. The growth characteristics and compatibility of the two strains are improved; the inhibition rate of cholesterol esterase and pancreatic lipase; the method comprises the following steps: evaluating the triglyceride removal rate, antioxidant activity, pathogenic bacterium resistance, artificially simulated gastrointestinal fluid tolerance, strain drug resistance and safety, compounding lactobacillus plantarum SR6 and enterococcus faecalis SM5 strains according to a ratio of 1: 1 to prepare a freeze-dried bacterial agent, and keeping good survival rate (pgt; 0.05) and functional characteristics, the strain can be used as a functional direct vat set starter and is directly applied to production of fermented dairy products, and the biological activity of food is improved.

The invention discloses an enterococcus faecium XL, and the preservation number of the enterococcus faecium XL is CGMCC (China General Microbiological Culture Collection Center) No. 28133. The enterococcus faecium XL and the application thereof have the following beneficial effects: 1, the enterococcus faecium XL provided by the invention can grow and metabolize under an oil reservoir condition to generate lactic acid organic acid, dissolve carbonate in a rock stratum, increase the permeability of the rock stratum, degrade heavy components in crude oil, relieve crude oil blockage in an oil layer, increase the liquid amount and improve the oil reservoir quality; the oil recovery rate is improved; 2, the enterococcus faecium XL is applied to microorganism single-well throughput, the average single-well oil increasing amount is greater than 400 tons, and the validity period is more than 200 days; and 3, the enterococcus faecium XL is applied to microbial oil displacement, the average daily oil increase of a well group is more than 5 tons, and the water content drop amplitude is more than 5%.

The invention relates to a biomarker for individual recognition and application thereof, and relates to the technical field of biology. The biomarker comprises at least one of the following microorganisms: propionibacterium acnes, staphylococcus epidermidis, marine paracoccus, streptococcus, rhodococcus fence celebrating, staphylococcus, pseudomonas, streptococcus sanguineus, achromobacter xylosoxidans, acinetobacter calcoaceticus, acinetobacter johnsonii, staphylococcus lentus, pseudomonas toralaris and brevundimonas vesicularis. Or enterococcus faecium. The biomarker can provide analysis with higher resolution in the aspect of differential strain information, has a better distinguishing effect, higher accuracy and sensitivity on individual recognition, and can be used as a new technical means for supplementing forensic individual recognition.

The invention discloses a humic acid type straw source microbial agent which comprises the following strains: enterococcus faecium, bacillus subtilis, lactobacillus plantarum and saccharomyces cerevisiae. The method has the advantages that turfy soil, biogas residues, biogas slurry and a certain amount of agricultural waste are added into compost with straw microbial agents and livestock and poultry manure as main materials to adjust a proper carbon nitrogen ratio, and more importantly, a compound microbial agent for strengthening the process of the humic acid type straw source microbial agent is inoculated; compared with natural composting fermentation, the composting efficiency and stability are improved, and the fertilizer efficiency and fertility of the organic fertilizer and the resource utilization efficiency of waste are improved.

The invention provides an application of GSK2018682 in preparation of a medicine for resisting gram-positive bacterial infection, and the GSK2018682 has a CAS number of 1034688-30-6. The invention also provides an application of the GSK2018682 in preparation of a medicine for resisting gram-positive bacterial infection. According to the technical scheme, the novel medical application of the GSK2018682 is disclosed, and the GSK2018682 has good antibacterial activity and anti-biofilm activity on various gram-positive bacteria and can inhibit growth of staphylococcus aureus, staphylococcus epidermidis, enterococcus faecalis, enterococcus faecium and the like and formation of biofilms.

The invention discloses a method for fermenting vinasse feed through mixed bacteria, and belongs to the field of microbial fermentation. The invention firstly provides a microbial agent for increasing the content of crude protein in fermented vinasse. The microbial agent contains a bacillus subtilis liquid, a corynebacterium glutamicum liquid, a saccharomyces cerevisiae liquid, an enterococcus faecalis liquid and a bifidobacterium animalis liquid. The invention also provides an application of the microbial agent in preparation of a fermented vinasse feed. The contents of crude protein, crude fat and amino acid in the fermented vinasse feed are increased. Through mixed fermentation, the content of amino acid in the vinasse is increased, the content of lysine is increased by 108.33%, and the content of threonine is increased by 59.09%.

The invention relates to the technical field of microorganisms, in particular to mixed bacteria fermentation liquor as well as a preparation method and application thereof. Bacterial strains in the mixed fermentation liquor comprise clostridium butyricum, enterococcus faecium and bacillus. The preparation method of the mixed bacteria fermentation liquor comprises the following steps: culturing the strains in the mixed bacteria fermentation liquor in a mixed bacteria fermentation culture medium to obtain the mixed bacteria fermentation liquor; wherein the bacterial strains comprise clostridium butyricum, enterococcus faecium and bacillus. According to the mixed bacterium fermentation liquor and the preparation method and application thereof, coexisting culture of donkey source enterococcus faecium, donkey source bacillus and donkey source clostridium butyricum can be achieved through the mixed bacterium fermentation liquor, and meanwhile the mixed bacterium fermentation liquor has the excellent effects of producing compound enzyme and inhibiting pathogenic bacteria and can serve as a feed additive to improve the reproductive performance of a female donkey and improve the growth performance of a donkey foal; the occurrence of donkey foal diarrhea is reduced, so that the economic benefit of donkey breeding is increased.

The invention provides an enterococcus faecium probiotic-prebiotics composite tablet and a preparation method thereof, and belongs to the technical field of microbial preparations. The tablet is prepared by taking enterococcus faecium (preservation number: CGMCC NO.33919) as a core active component, matching prebiotics adapted to the intestinal tracts of old people, a high-stability protective agent and mild auxiliary materials through a specific process. The tablet can efficiently adjust the intestinal flora structure of the old people, inhibit the proliferation of harmful bacteria in the intestinal tract and enhance the intestinal barrier function of the old people, so that the functional diarrhea and antibiotic-related diarrhea of the old people are relieved, and the tablet is moderate in hardness, easy to swallow, free of intestinal irritation and high in normal-temperature storage stability; the problems that an existing intestinal preparation is poor in adaptability to intestinal tracts of old people, strain activity is prone to inactivation, and swallowing is difficult are solved.

The invention discloses an enterococcus faecalis bacteriophage vBEfaPFA2, a bacteriophage composition and application of the enterococcus faecalis bacteriophage vBEfaPFA2, the enterococcus faecalis bacteriophage vBEfaPFA2 has a specific lysis effect on enterococcus faecalis and also has a cross-species lysis effect on salmonella, the acid-base adaptability of the bacteriophage is high, and the bacteriophage vBEfaPFA2 and the bacteriophage composition can be used for preparing the enterococcus faecalis bacteriophage The enterococcus faecalis bacteriophage can be used as an active component to be prepared into the bacteriophage composition or the bacteriophage pharmaceutical preparation, the bacteriophage composition or the bacteriophage pharmaceutical preparation is applied to preparation of a medicine or a bacteriostatic agent for preventing and treating enterococcus faecalis and salmonella infection, the enterococcus faecalis bacteriophage and the bacteriophage composition are safe to use, high in yield and easy to industrially produce, and due to the cross-species lysis performance, the bacteriophage can be The method has the advantages of wide splitting spectrum, wide application range and good application prospect.

The invention discloses a walnut probiotic fermented beverage and a preparation method thereof. The beverage is prepared from walnut kernels as a preparation raw material through probiotic fermentation. The walnut kernels are used as preparation raw materials, the beverage is prepared through probiotic fermentation, and the beverage has the advantages of being good in taste, rich in nutrient substance, high in oxidation resistance and the like. The lactobacillus plantarum subsp. Plantarum RF17 and the lactobacillus rhamnosus GOLDGUT-M520 are combined for fermentation, so that the content of total flavonoids in the fermented beverage is increased, and the enterococcus faecalis 2010-JR-01 and the lactobacillus paracasei LCX25 are combined for fermentation, so that the content of total phenols in the fermented beverage is increased.

The invention provides an antibacterial peptide RB3 and application thereof. The amino acid sequence of the antibacterial peptide RB3 is as shown in SEQ ID NO: 1. The antibacterial peptide RB3 has remarkable antibacterial activity on five endometritis pathogenic bacteria, namely, E. coli, K. pneumoniae, P. mirabilis, P. aeruginosa and E. faecium, can be used for inhibiting the growth and proliferation of the endometritis pathogenic bacteria and maintaining a normal flora structure and an immune microenvironment of endometrium, and can be used for preparing the antibacterial peptide RB3. The antibacterial peptide RB3 has the advantages that the antibacterial peptide RB3 has obvious antibacterial activity on the five endometritis pathogenic bacteria, namely, E. coli, K. pneumoniae, P. mirabilis, P. aeruginosa, P. mirabilis, P. aeruginosa and E. faecium; according to the present invention, the antibacterial peptide RB3 can be directly obtained through solid-phase synthesis, such that the effective prevention and treatment of endometritis can be achieved, the important significance on the prevention and the clinical treatment of endometritis can be provided, and the antibacterial peptide RB3 can be directly obtained through solid-phase synthesis so as to easily achieve the large-scale preparation and the clinical large-scale application of the antibacterial peptide RB3.

The invention provides a beta-diketoamide compound and application thereof in preparation of a gram infection resistant drug, the structural formula of the beta-diketoamide compound is as shown in formula (1), in the formula (1), R1 is H or halogen; r2 is nitro, alkyl, halogen or methoxyl. The beta-diketoamide compound disclosed by the invention comprises gram-positive bacteria such as staphylococcus aureus, enterococcus faecalis, enterococcus faecium and staphylococcus epidermidis and other clinical isolates, and shows antibacterial activity; after being combined with polymyxin, the compound has synergistic bacteriostatic activity on gram-negative bacteria such as acinetobacter baumannii, klebsiella pneumoniae, escherichia coli and pseudomonas aeruginosa, and can remove biofilms formed by staphylococcus aureus and enterococcus faecalis.

The use of a probiotic, particularly the Enterococcus faecium strain NCIMB 10415 which is named SF68® as probiotic active ingredient, in combination with butyric acid or a salt thereof to increase the enterocyte's health and consequently the wellness of pets and livestock is described.

The invention provides a natto food antibacterial preservative, a preparation method and application, and belongs to the technical field of food preservation, the preservative is mainly prepared from the following raw materials: mixed beans, protease, bacillus natto and enterococcus faecium; wherein the mixed beans consist of the following components in parts by mass: 30-50 parts of soybeans, 40-50 parts of peas, 5-10 parts of oats and 10-20 parts of wheat; the use amount of the protease is 0.1-0.5% of the total mass of the mixed beans; the use amount of the bacillus natto is 1%-3% of the total mass of the mixed beans; the dosage of the enterococcus faecium is 1%-3% of the total mass of the mixed beans. Through scientific compatibility, the antibacterial and preservative effects of the natto paste are improved, and the technical blank of organic natto preservatives in the field of long-acting bacteriostasis of food bodies is filled.

The invention discloses an antibacterial peptide sesquidendrin and an application of the antibacterial peptide sesquidendrin. The invention specifically relates to eight sesquistigma polypeptide compounds. The compounds 1-8 show different degrees of antibacterial activity, the compound 2 has broad-spectrum antibacterial activity, the minimum inhibitory concentration of the compound 2 to staphylococcus aureus ATCC 6538 and bacillus cereus CMCC (B) 63301 reaches 20 [mu] g / mL, and the MIC value of the compound 2 to enterococcus faecium 160119481, salmonella typhimurium CMCC (B) 50071 and salmonella typhimurium CMCC (B) 50115 is 40 [mu] g / mL. As staphylococcus, bacillus, enterococcus and salmonella bacteria are common food-borne pathogenic bacteria which seriously threaten food safety, the compounds 1-8 can be used for preparing novel antibacterial peptides which are applied to the field of food safety preservation.

This invention relates to the field of biotechnology, specifically disclosing a freeze-drying protection system for Enterococcus faecalis, its preparation method, and its applications. The freeze-drying protection system comprises arginine, skim milk, and glycerol, combined with low-temperature stress pretreatment. The freeze-drying protection system provided by this invention significantly increases the freeze-drying survival rate of Enterococcus faecalis L118 to 87.90±1.28%, and the survival rate remains above 90% after 28 days of storage at -20℃. The core probiotic characteristics of the freeze-dried Enterococcus faecalis L118 strain, such as acid production capacity, simulated gastric juice, and NaCl tolerance, are well preserved, providing an effective solution for preparing highly active and stable Enterococcus faecalis preparations.

A bithionol derivative, and a preparation method therefor and a use thereof. The bithionol derivative is as represented by any one of chemical structural formulas I-1 to I-7. The bithionol derivative as represented by the structural formula exhibits better bacteriostatic activity against clinical isolates of gram-positive bacteria such as Staphylococcus aureus, Enterococcus faecalis, Enterococcus faecium, and Staphylococcus epidermidis and the effect of inhibiting the formation of Staphylococcus aureus biofilms. As compared with bithionol and other bithionol derivatives, the bithionol derivative as represented by the structural formula has a lower inhibitory concentration, a better antibacterial effect, and potential application value in clinical antibacterial infection treatment.

The application discloses a cat oral cavity nursing probiotic composition and application thereof, and the probiotic composition comprises animal bifidobacterium and lactic acid bacteria, wherein the lactic acid bacteria is any one or a combination of multiple of salivarius, lactobacillus casei, enterococcus faecalis, lactobacillus rhamnosus, lactobacillus plantarum, lactobacillus acidophilus and lactobacillus reuteri; compared with single probiotic, the probiotic composition has better effect on oral cavity odor removal, can effectively improve cat oral cavity source halitosis, and can also reduce dental plaque formation, relieve gingivitis and dental caries, and can be applied to preparation of a cat oral cavity odor cleaner, and has good application prospect.

The invention provides a harmless treatment method and application of chicken manure, and relates to the technical field of waste recycling. Specifically, the method comprises an ozone oxidation process, a drying process, an enzymolysis process, an aerobic fermentation process and an anaerobic fermentation process which are carried out in sequence, wherein in the aerobic fermentation process, a pre-product is mixed with charcoal, a cellulose source, plant ash, wine grape skin and rice bran meal for fermentation; in the anaerobic fermentation process, compound bacteria including clostridium butyricum, pediococcus acidilactici, bacillus coagulans and enterococcus faecium are added into a pre-product, and sealed fermentation is performed. The chicken manure is subjected to targeted detoxification treatment according to the characteristics of the chicken manure, pathogenic microorganisms and complex heavy metals in the chicken manure can be effectively removed, the content of mycotoxin in the chicken manure is reduced, nutrient substances in the chicken manure are decomposed, resource application in the field of fertilizer or feed is achieved, and the method has good application prospects.

The invention belongs to the technical field of PCR detection, and particularly relates to a composition for detecting pathogenic bacteria, a kit, application and a detection method. The pathogenic bacteria are any one or more of enterococcus faecalis, escherichia coli, klebsiella pneumoniae, enterobacter cloacae, enterococcus faecium and shigella flexneri pathogens, and the composition comprises any one or more of a component A, a component B, a component C, a component D, a component E and a component F; the composition comprises forward and reverse primers and probes aiming at enterococcus faecalis, escherichia coli, klebsiella pneumoniae, enterobacter cloacae, enterococcus faecium and shigella flexneri. The composition provided by the invention can simultaneously detect and distinguish enterococcus faecalis, escherichia coli, klebsiella pneumoniae, enterobacter cloacae, enterococcus faecium and shigella flexneri, has high sensitivity, specificity and precision, is helpful to take treatment measures in time and customize personalized treatment schemes, reduces unnecessary use of antibiotics, and has a good application prospect. The generation of drug resistance is reduced.