The invention discloses a kit for detecting genetically modified soybean in the field of test items. The kit for detecting genetically modified soybean comprises a primer combination and a membrane chip. The kit for detecting genetically modified soybean is characterized in that the primer combination is a seven polymerase chain reaction (PCR) primer combinations; each pair of primers comprise Pat, Bar, Cry1Ab, Cry105, EPSPS, P35S and a soybean endogenous gene sequence Lectin; the membrane chip is seven groups of probe sequences, which are orderly fixed on the surface of a support membrane and a group of positive control probe sequences, the probe sequences are respectively a Pat probe, a Bar probe, a Cry1Ab probe, a Cry105 probe, an EPSPS probe, a P35S probe, a Lectin probe and a Positive probe; an asymmetric PCR amplification primer is formed in the primer combination, and is a Tag primer with a biotin marker (biotin) at the 5' end. By adopting the kit for detecting genetically modified soybean, the defects of time and labor waste, and high cost in the prior art are overcome; the provided kit for detecting genetically modified soybean is simple to operate, quick and sensitive, large in detection throughput, visual in detection result and low in cost.