3 results about "Asymmetric PCR" patented technology

The present invention relates to methods and compositions for preparing a sample enriched for single stranded nucleic acids. The single stranded nucleic acids find use as templates for synthesizing Xpandomer copies of the nucleic acids. The Xpandomer copies may be passed through a nanopore sensor to determine the nucleic acid sequence of the nucleic acids. The methods include asymmetric PCR, amplicon immobilization melt, exonuclease-mediated partial or complete digestion of one or both strand of an amplicon and linear amplification.

The application discloses an ABO genotyping composition and kit based on a multiple asymmetric PCR probe melting curve and application. The application can realize accurate ABO genotyping in one batch of PCR reactions, and the whole detection process is simple, rapid, accurate and efficient, and has high practical value. The application makes up for the shortcomings of the existing serological detection method, has the advantages of standardization, low cost and low technical barrier for clinical popularization, and has a wide application prospect in ABO genotyping for Chinese population.

The present application relates to the application of graphene oxide in the preparation of single-stranded DNA secondary library for aptamer screening. Specifically, graphene oxide is incubated with the product after asymmetric PCR amplification, and the single-stranded DNA secondary library on graphene oxide can be obtained after centrifugation and washing. The method of the present application is simple, does not affect the specificity and affinity of aptamer, has the advantages of low cost and high efficiency, overcomes the problems of existing single-stranded DNA secondary library preparation methods, such as many steps, high operation requirements and high cost, and helps to screen high-quality aptamer.