10 results about "Bacterial inhibition" patented technology

The present invention discloses a method for detecting the inhibition of antibiotics against bacteria, a bacteria counting device, and the method. The inhibition detection method includes the steps of: adding a predetermined concentration of antibiotic to a target bacterium to form a bacteria-drug mixture; and using a target bacterium without the antibiotic as a positive control; acquiring the current number of the bacteria in the bacteria-drug mixture and the current number of the bacteria in the positive control at a first predetermined time interval after the antibiotic is added; and determining whether the predetermined concentration of antibiotic inhibits, partially inhibits, or does not inhibit the bacteria based on the ratio of the current number of the bacteria in the bacteria-drug mixture to the current number of the bacteria in the positive control. The method is suitable for rapid drug susceptibility testing.

The application discloses a bacterial QS inhibitor and application thereof, and the active ingredient of the bacterial QS inhibitor is apigenin, and the use concentration is a sub-inhibitory concentration of target bacteria. The application first discovers and systematically proves that apigenin has a wide and significant inhibitory effect on a variety of bacterial QS systems and a spoilage phenotype regulated by the QS systems. Experiments show that under a sub-inhibitory concentration, the inhibition rate of apigenin on biofilm formation of pectobacterium is up to 96.33%, the inhibition rate of apigenin on synthesis of a signal molecule is up to 88.42%, and the inhibition rate of apigenin on a key virulence enzyme is more than 70%; the fresh-keeping effect of apigenin on food is significant, and the application of apigenin in the field of food fresh-keeping is expanded; and apigenin is a natural dietary ingredient and has high safety.

Compositions and methods are described demonstrating the ability to 1) use a virus assisted directed evolution platform to significantly improve the activity of engineered nonsense-suppressor tRNAs in mammalian cells, 2) provide mutants of E. coli tyrosyl-tRNAs that show remarkably improved Uaa incorporation efficiency in mammalian cells, and 3) use these tRNAs to express recombinant proteins in mammalian cells incorporating Uaas at significantly improved yields.

The invention discloses a nano-scale silver ion and traditional Chinese medicine extract composite bactericide, and relates to the technical field of composite bactericides, the nano-scale silver ion and traditional Chinese medicine extract composite bactericide comprises 30 g of sophora flavescens, 10 g of phellodendron, 30 g of glabrous greenbrier rhizome, 15 g of common cnidium fruit, 10 g of oriental wormwood, 10 g of honeysuckle, and 20 g of silver ions with a silver content of 5000 ppm, and 1000 ml of the nano-scale silver ion and traditional Chinese medicine extract composite bactericide is prepared. Silver ions are positively charged cations formed when metal silver loses one or more electrons and comprise Ag < 1 + >, Ag < 2 + > and Ag < 3 + > which exist in the form of an aqueous solution, and the preparation method of the nano-scale silver ion and traditional Chinese medicine extract composite bactericide comprises the following steps that S1, six traditional Chinese medicines including radix sophorae flavescentis, cortex phellodendri, rhizoma smilacis glabrae, fructus cnidii, herba artemisiae scopariae and honeysuckle flowers are taken and extracted with water for use. The traditional Chinese medicine composition has a good killing effect on vibrio, mould, aeromonas hydrophila and the like through the combination of the traditional Chinese medicine and the silver ions, compared with a single preparation, the dosage of the traditional Chinese medicine composition is better than that of a single preparation, and the traditional Chinese medicine composition is better in killing bacteria and inhibiting drug resistance of viruses and is higher in cost performance.

This invention relates to the field of *Paenibacillus polymyxa*, addressing the problem of low production of immune signaling proteins in existing *Paenibacillus* strains. It provides a new *Paenibacillus polymyxa* strain, deposited at the China General Microbiological Culture Collection Center (CGMCC) with accession number CGMCC NO.36323. This invention provides a novel *Paenibacillus* strain that possesses both high production of immune signaling proteins and natural biocontrol and growth-promoting properties. It can secrete polymyxin-like antibacterial substances and simultaneously synthesize PR proteins and selenomethionine, overcoming the limitations of traditional *Paenibacillus* strains that only inhibit bacterial growth and lack immune signal transduction. The preparation method provided by this invention involves the strain converting sodium selenite into nano-selenium, which combines with the secreted PR protein to form nano-selenoprotein. Simultaneously, it synergistically produces polymyxins, achieving a dual effect of immune signal activation and direct bacterial inhibition, solving the problems of weak signaling and lack of synergistic antibacterial effects of traditional inducers.

The invention relates to the technical field of insole preparation, in particular to a sterilization physiotherapy insole and a preparation method thereof. The preparation method comprises the following steps: preparing an upper composite insole layer; preparing a power supply control pad; preparing a lower supporting layer; the upper composite insole layer, the power control layer and the lower supporting layer are sequentially subjected to hot-pressing composition, and the sterilization physiotherapy insole is obtained. And the preparation raw materials of the upper composite insole layer comprise titanium dioxide and a micro ultraviolet LED. In the application, the upper composite insole layer, the power supply control pad and the lower supporting layer are prepared step by step, and then the functional layers are integrated through hot-pressing compounding, titanium dioxide in the upper composite insole layer is matched with a micro ultraviolet LED to play a role in bacteriostasis, and in combination with the synergistic effect of the subsequent functional layers, the functions of sterilization and physical therapy can be integrated; moreover, an irritant antibacterial agent is not needed, the use safety and the health care effect are both considered, and the comprehensive requirements of daily wearing on foot bacterium inhibition and acupuncture point care are met.

The invention belongs to the technical field of polymer functional materials, and particularly relates to a PPS antibacterial composite fiber membrane and a preparation method thereof.The composite fiber membrane is composed of a PPS matrix membrane, a PDA initiation layer wrapping the outer side of the PPS matrix membrane and an Ag-loaded ZIF functional layer combined with the PDA initiation layer, and the Ag-loaded ZIF functional layer is an Ag-loaded Cu and Zn bimetal ZIF functional layer. The composite fiber membrane has obvious structural characteristics, combines the initiation adhesion layer of PDA, the porous structure of ZIF (Zn / Cu) and the antibacterial property of Ag nanoparticles, and has excellent performance in bacterial inhibition and durability application by relying on the high mechanical strength and chemical stability of the PPS base membrane.

The present invention relates to direct feeding microorganisms for inhibiting Escherichia coli, Salmonella and / or Clostridium in an animal. More specifically, the present invention relates to isolated Bacillus strains CM256 (NRRL number B-67706), CM857 (NRRL number B-67705), CM978 (NRRL number B-67703), CM1004 (NRRL number B-67708) and MDG1607 (NRRL number B-67666), as well as to the use of strains having all of the identifying characteristics of these strains, comprising the above use. The present invention also relates to isolated Bacillus strains CM256 (NRRL number B-67706), CM857 (NRRL number B-67705), CM978 (NRRL number B-67703) and CM1004 (NRRL number B-67708) and the use of strains having all identifying characteristics of these strains in the treatment of plants suffering from a disease caused by E. coli, Salmonella and / or Clostridium.

The invention belongs to the technical field of human body lubricants, and particularly relates to a preservative-free human body lubricant and a preparation method thereof. The lubricant comprises the following components in percentage by mass: 60%-75% of polyol, 5%-10% of betaine, 0.1%-0.5% of hyaluronic acid, 0.5%-2% of a thickening agent and the balance of deionized water. The preparation method comprises the following steps: mixing and stirring under specific conditions; according to the invention, a high osmotic pressure system is constructed by polyol and betaine to inhibit the growth of bacteria, the diameters of inhibition zones on escherichia coli and other bacteria are greater than or equal to 12.0 mm, the moderate and strong antibacterial grade is reached, and a sterile production environment and a special sterilization process are not needed in the preparation process; and compared with the existing preservative-free lubricant, the production period is shortened by more than 25%, and the production cost is reduced by 30-40%.

The application belongs to the technical field of marine chemical engineering, and particularly relates to N,N,N-trimethyl chitosan quaternary ammonium salt containing glycine Schiff base, a preparation method and application thereof. The N,N,N-trimethyl chitosan quaternary ammonium salt containing glycine Schiff base is shown in formula (1). The application has the advantages of simple reaction equipment, easy control of reaction process, low cost of raw materials, high safety, easy popularization and industrial application. Research proves that the compound has good antioxidant activity and bacterial inhibition activity, and can be applied to the fields of agriculture, medicine and environmental protection.