35 results about "Bacterial inhibition" patented technology

The invention discloses a bacterial inhibitor for sea bass preservation, the bacterial inhibitor is bacteriocin generated by fermentation of lactobacillus plantarum L13 (the preservation number is CGMCC NO.27398), the bacteriocin comprises active polypeptide xylAB, the amino acid sequence of the xylAB is LLSSLATTGAIALGATAAKA, the preservation number of the bacterial inhibitor is CGMCC NO.27398, the preservation number of the bacterial inhibitor is CGMCC NO.27398, and the preservation number of the bacterial inhibitor is CGMCC NO.27398. The fresh-keeping agent prepared by the invention has a good fresh-keeping effect, can prolong the fresh-keeping period, and has huge potential as a natural food preservative in the food industry.

The invention discloses a method for promoting photodegradation of coexisting ciprofloxacin and reducing ecological toxicity of the coexisting ciprofloxacin by active oxygen generated by photoaging polystyrene microplastics. The polystyrene micro-plastic PS-MPs is subjected to light irradiation to be aged to a certain degree, and surface physicochemical property changes of the PS micro-plastic PS-MPs are represented through SEM, FTIR and XPS. Especially in the coexistence of aged PS, the degradation rate (67%) of CIP is almost three times of the degradation rate (21%) of CIP in the presence of original PS. Quenching experiments and steady-state concentration experiments prove that the enhancement of CIP photodegradation by aged PS-MPs is related to reactive oxygen species (ROS) generated on the surfaces of the aged PS-MPs, and < 1 > O2 plays a leading role in the photodegradation process of CIP. Bacteria inhibition experiments prove that in the presence of aged PS, the biotoxicity of CIP is weakened. The method also shows a relatively strong application effect in actual water bodies.

The application discloses a microbial drug sensitivity detection method. The application comprises: loading a microbial database, establishing a corresponding relationship between secondary microbial names and primary categories; loading a drug breakpoint database based on the "antimicrobial drug sensitivity experiment execution standard"; placing a standard culture dish in an image acquisition device to acquire an image, and calibrating an image analysis system with a vernier caliper; inputting sample information to be detected, acquiring and uploading the image; an image analysis system extracts the position of a drug sensitivity paper sheet, identifies the edge of a bacterial inhibition zone and calculates the diameter; the system extracts corresponding drug breakpoint thresholds based on the microbial category, compares the measured diameter and determines the sensitivity classification (S / I / R); and finally, the result is uploaded to a LIS system. The method can realize the automation, standardization and high efficiency of drug sensitivity detection.

The present invention discloses a sesquiterpene glycoside compound and its preparation method and application. A new sesquiterpene glycoside ligulariatinside A is isolated and identified from the n-butanol extraction part of 95% ethanol extract from the root of Ligularia glossata by multiple chromatographic methods such as macroporous resin column chromatography, positive and reverse phase silica gel column chromatography and high performance liquid chromatography, as well as spectral methods. The molecular formula of the new sesquiterpene glycoside ligulariatinside A is determined to be C 21 H 34 O7, the structure is (3 S ,4 R ,5 R ,7 R )‑11‑hydroxy‑11,12‑dihydronootkatone‑11‑ O ‑ β ‑D‑glucopyranoside enriches the chemical diversity of Ligularia officinalis. Bacterial inhibition experiments revealed that the new sesquiterpenoid glycoside exhibits inhibitory activity against Vibrio anguillarum, with a minimum inhibitory concentration (MIC) of 50 μg / mL, laying the foundation for the development of new antibacterial drugs.

The invention relates to the technical field of polymer membrane preparation, and particularly discloses a preparation method, equipment and medium of a recoverable super-hydrophilic PVDF composite membrane, and the preparation method comprises the following steps: S1, preparing a polymer base material; s2, carrying out dopamine functionalization treatment; s3, carrying out aldehyde group carbon dot-polycation grafting; s4, carrying out epoxy cross-linking curing; s5, shaping and post-processing; according to the composite membrane prepared by the preparation method disclosed by the invention, an oil-water separation test shows that the modified M-1. 5 membrane has excellent oil-water separation capacity, and the membrane still keeps stable separation flux after five times of separation circulation; an antibacterial test shows that an obvious bacterial inhibition ring is found around M-1. 5, it is indicated that the M-1. 5 composite membrane can prevent bacteria from approaching and inhibit growth of escherichia coli, a repairable test shows that PVDF-3, PAN-7 and PES-3 membranes have repairable performance, and the proposal of the scheme provides a new thought for rapid repair of damaged polymer membranes.

The invention provides a non-dissolution type antibacterial dressing and a preparation method thereof, and relates to the technical field of medical dressings. A non-dissolution type antibacterial dressing comprises a base material, micron-sized antibacterial microspheres and an endotoxin adsorption material, and the antibacterial microspheres and the endotoxin adsorption material are loaded inside and / or on the surface of the base material in one or more modes of physical wrapping, physical winding and chemical bond connection; the base material comprises polyvinyl alcohol fibers and / or chitosan sponge; the endotoxin adsorption material comprises modified styrene divinyl benzene affinity resin nano-structure particles and / or poly (4-methyl-1-pentene) fibers. The antibacterial microspheres can effectively kill bacteria at wounds and inhibit growth and reproduction of the bacteria, the endotoxin adsorption material has strong adsorption capacity, inflammatory response caused by endotoxin is reduced, the risk of wound infection is reduced, the antibacterial microspheres and the endotoxin adsorption material are mutually synergistic in performance, the antibacterial performance is excellent, wound adhesion is avoided, the risk of wound infection is reduced, and the wound healing effect is good. The wound healing is promoted.

The invention belongs to the technical field of microbial prevention and control of plant diseases and insect pests, and particularly discloses a plant disease biocontrol bacillus cereus strain LCBC246 which is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number of the strain is CGMCC No.33323. The invention further discloses a preparation method of the plant disease biocontrol bacillus cereus strain LCBC246. The LCBC246 strain provided by the invention is a gram-positive bacterium, has a very strong bacteriostatic effect on colletotrichum fucicola, the bacteriostatic rate is 92.1%, the disease control rate on the colletotrichum fucicola is 90.8%, and the LCBC246 strain has a very good inhibitory effect on other important plateau characteristic crop pathogenic bacteria and also has a remarkable crop growth promoting property. The bacterial inhibition rates of fermentation liquor and volatile substances of the strain LCBC246 on colletotrichum gloeosporioides of macadamia nuts are 62.70% and 76.58% respectively; the bacillus amyloliquefaciens can generate sterilization metabolites such as cellulase, amylase and siderophore, and has a very strong broad-spectrum bacteriostatic effect on various plant pathogenic fungi. Therefore, the strain LCBC246 and the metabolite thereof have the application value of developing bacterial biopesticides.

The invention relates to the technical field of cosmetics, and discloses a deodorization cosmetic with bacteriostatic and soothing effects and a preparation method of the deodorization cosmetic. 3%-7% of persimmon fruit extract; 12%-15% of aloe gel; 4%-7% of glycerol; 7%-11% of a chamomile extract; 5%-8% of menthol; 2%-6% of a cordate houttuynia extract; 5%-9% of amino acid; 7%-10% of zinc chloride; 4%-8% of a vitamin combination; 1%-3% of beewax; 3%-6% of rose water; 0.5%-1% of corn starch; 2.5%-4% of sodium hyaluronate; according to the present invention, the deodorizing effect is achieved through the reasonable ratio of the menthol, the zinc chloride and the heartleaf houttuynia herb extract, the cooling feeling of the menthol can cover the odor, the zinc chloride can inhibit the growth of the microorganisms generating the odor, and the heartleaf houttuynia herb extract can increase the natural antibacterial property of the deodorizing cosmetic; the combination of the three raw materials can destroy the bacterial structure and inhibit metabolism by bacteria, so that the generation of peculiar smell is reduced from the source.

The invention belongs to the technical field of biological medicines, and particularly relates to a pharmaceutical composition, which comprises: (a) carnosine; and (b) at least one antibiotic selected from the group consisting of aminoglycoside antibiotics and polymyxin antibiotics. Researches find that carnosine has the effect of enhancing the bactericidal effect of aminoglycoside antibiotics or polymyxin antibiotics, carnosine and the two antibiotics are jointly used for resisting bacterial infection, and the carnosine and the two antibiotics have a remarkable synergistic effect and can jointly act to achieve the remarkable bacterial inhibition effect which cannot be achieved by a single antibiotic.

The invention relates to direct-fed microbials for use in E. coli, Salmonella, and / or Clostridium inhibition in animals. More particularly, the invention relates to isolated Bacillus strains CM256 (NRRL No. B-67706), CM857 (NRRL No. B-67705), CM978 (NRRL No. B-67703), CM1004 (NRRL No. B-67708), and MDG1607 (NRRL No. B-67666), and strains having all of the identifying characteristics of these strains, for a use comprising the above-mentioned use. The invention also relates to the use of isolated Bacillus strains CM256 (NRRL No. B-67706), CM857 (NRRL No. B-67705), CM978 (NRRL No. B-67703), and CM1004 (NRRL No. B-67708), and strains having all of the identifying characteristics of these strains, to treat plants having diseases caused by E. coli, Salmonella, and / or Clostridium.

The present invention discloses a method for detecting the inhibition of antibiotics against bacteria, a bacteria counting device, and the method. The inhibition detection method includes the steps of: adding a predetermined concentration of antibiotic to a target bacterium to form a bacteria-drug mixture; and using a target bacterium without the antibiotic as a positive control; acquiring the current number of the bacteria in the bacteria-drug mixture and the current number of the bacteria in the positive control at a first predetermined time interval after the antibiotic is added; and determining whether the predetermined concentration of antibiotic inhibits, partially inhibits, or does not inhibit the bacteria based on the ratio of the current number of the bacteria in the bacteria-drug mixture to the current number of the bacteria in the positive control. The method is suitable for rapid drug susceptibility testing.

The application discloses a bacterial QS inhibitor and application thereof, and the active ingredient of the bacterial QS inhibitor is apigenin, and the use concentration is a sub-inhibitory concentration of target bacteria. The application first discovers and systematically proves that apigenin has a wide and significant inhibitory effect on a variety of bacterial QS systems and a spoilage phenotype regulated by the QS systems. Experiments show that under a sub-inhibitory concentration, the inhibition rate of apigenin on biofilm formation of pectobacterium is up to 96.33%, the inhibition rate of apigenin on synthesis of a signal molecule is up to 88.42%, and the inhibition rate of apigenin on a key virulence enzyme is more than 70%; the fresh-keeping effect of apigenin on food is significant, and the application of apigenin in the field of food fresh-keeping is expanded; and apigenin is a natural dietary ingredient and has high safety.

Compositions and methods are described demonstrating the ability to 1) use a virus assisted directed evolution platform to significantly improve the activity of engineered nonsense-suppressor tRNAs in mammalian cells, 2) provide mutants of E. coli tyrosyl-tRNAs that show remarkably improved Uaa incorporation efficiency in mammalian cells, and 3) use these tRNAs to express recombinant proteins in mammalian cells incorporating Uaas at significantly improved yields.

The utility model relates to the technical field of total heat exchangers, and discloses an intelligent total heat exchanger which comprises a box body, a first air inlet and a first air outlet are formed in the outer surface of the front end of the box body, and the first air inlet is located on one side of the first air outlet. The blue antibacterial layer is arranged in the filter screen, growth of bacteria can be inhibited, excessive breeding of bacteria can be prevented, viruses and bacteria entering the total heat exchanger can be effectively killed through the photo-hydrogen ion sterilizer and the two-in-one filter screen, growth of the bacteria in the total heat exchanger is inhibited, spread infection of the bacteria on large places again is reduced, and the service life of the total heat exchanger is prolonged. Through the arrangement of the first connecting plate, the second connecting plate and the third connecting plate, the first two-in-one filter screen, the second two-in-one filter screen and the third two-in-one filter screen can be conveniently and rapidly installed in the box body of the total heat exchanger, and installation or disassembly is more convenient and rapid.

The invention discloses a nano-scale silver ion and traditional Chinese medicine extract composite bactericide, and relates to the technical field of composite bactericides, the nano-scale silver ion and traditional Chinese medicine extract composite bactericide comprises 30 g of sophora flavescens, 10 g of phellodendron, 30 g of glabrous greenbrier rhizome, 15 g of common cnidium fruit, 10 g of oriental wormwood, 10 g of honeysuckle, and 20 g of silver ions with a silver content of 5000 ppm, and 1000 ml of the nano-scale silver ion and traditional Chinese medicine extract composite bactericide is prepared. Silver ions are positively charged cations formed when metal silver loses one or more electrons and comprise Ag < 1 + >, Ag < 2 + > and Ag < 3 + > which exist in the form of an aqueous solution, and the preparation method of the nano-scale silver ion and traditional Chinese medicine extract composite bactericide comprises the following steps that S1, six traditional Chinese medicines including radix sophorae flavescentis, cortex phellodendri, rhizoma smilacis glabrae, fructus cnidii, herba artemisiae scopariae and honeysuckle flowers are taken and extracted with water for use. The traditional Chinese medicine composition has a good killing effect on vibrio, mould, aeromonas hydrophila and the like through the combination of the traditional Chinese medicine and the silver ions, compared with a single preparation, the dosage of the traditional Chinese medicine composition is better than that of a single preparation, and the traditional Chinese medicine composition is better in killing bacteria and inhibiting drug resistance of viruses and is higher in cost performance.

The invention belongs to the technical field of biology, and discloses a phytobacterium atrati TG004, an application of the phytobacterium atrati TG004 in preparation of cariogenic bacterium inhibitors or 5-hydroxytryptophan, and a microbial marker related to gastric cancer. According to the present invention, with the application of the Argentlat lactobacillus TG004, the activity and the biological membrane formation process of cariogenic bacteria such as streptococcus mutans, streptococcus mutans and porphyromonas gingivalis can be well inhibited, and the Argentlat lactobacillus TG004 has excellent potential of being used as the cariogenic bacteria inhibitor, and has important significance on the prevention and treatment of caries.

The utility model discloses an antibacterial knitted fabric, which relates to the technical field of fabrics and comprises a fabric surface layer, a fabric middle layer and a fabric base layer, the fabric middle layer is arranged between the fabric surface layer and the fabric base layer, a plurality of surface layer air holes are arranged on the inner side of the fabric surface layer, and the surface layer air holes are arranged on the outer side of the fabric base layer. A plurality of middle layer air holes are formed in the inner side of the fabric middle layer, and a plurality of base layer air holes are formed in the inner side of the fabric base layer. By the adoption of the structure, the linen fiber gauze layer can play a good antibacterial and anti-mite effect on the outside, secondly, the first kapok fiber layer and the second kapok fiber layer in the fabric base layer are not beneficial to survival of anaerobic bacteria, meanwhile, kapok fibers have an obvious inhibiting effect on escherichia coli, the mite repelling effect is obvious, and thirdly, the anti-bacterial and anti-mite effects are achieved. In the bamboo charcoal fiber layer, the antibacterial ability and the diversified antibacterial effect of the knitted fabric can be further enhanced through natural sterilization inhibition of bamboo fibers.

The invention relates to the technical field of biology, in particular to application of bacillus velezensis F3A and wettable powder to prevention and treatment of Lanzhou lily fusarium wilt and application of the bacillus velezensis F3A and wettable powder to promotion of Lanzhou lily growth, the bacillus velezensis F3A is preserved in China General Microbiological Culture Collection Center on December 21, 2020, the preservation number is CGMCC No.21518, and the preservation number is CGMCC No.21518; the bacterial inhibition rate of the bacillus velezensis F3A on the Lanzhou lily wilt pathogenic bacterium fusarium tripterygium reaches 75.23%, and fermentation liquor effectively inhibits mycelial growth and spore germination of the fusarium tripterygium and can be stably colonized at the root system and bulb of the Lanzhou lily. The invention discloses a formula of bacillus velezensis F3A wettable powder. The bacillus velezensis F3A wettable powder disclosed by the invention has a relatively good growth promoting effect on Lanzhou lilium brownii, can be used for effectively preventing and treating fusarium wilt, and has a relatively good market application prospect.

The invention relates to the technical field of antibacterial plastics, and particularly discloses an antibacterial plastic alloy applied to baby products and a preparation method of the antibacterial plastic alloy. The antibacterial plastic alloy applied to the baby products is prepared from the following raw materials based on the total mass of the antibacterial plastic alloy: a resin material, a lubricant, a compatilizer, a catalyst, a composite antibacterial agent and an antioxidant, based on the total mass of the composite antibacterial agent, the composite antibacterial agent is prepared from the following raw materials in parts by weight: nano zirconium phosphate loaded zinc, carbon nanotubes, polyhexamethylene biguanide, decyl dimethyl ammonium chloride and lauryl sodium sulfate. The antibacterial plastic alloy prepared by adopting the preparation method disclosed by the invention has the highest bacteriostasis rates of 99.99% and 99.97% on staphylococcus aureus and escherichia coli, and the antibacterial property of the antibacterial plastic alloy is improved.

This invention relates to the field of *Paenibacillus polymyxa*, addressing the problem of low production of immune signaling proteins in existing *Paenibacillus* strains. It provides a new *Paenibacillus polymyxa* strain, deposited at the China General Microbiological Culture Collection Center (CGMCC) with accession number CGMCC NO.36323. This invention provides a novel *Paenibacillus* strain that possesses both high production of immune signaling proteins and natural biocontrol and growth-promoting properties. It can secrete polymyxin-like antibacterial substances and simultaneously synthesize PR proteins and selenomethionine, overcoming the limitations of traditional *Paenibacillus* strains that only inhibit bacterial growth and lack immune signal transduction. The preparation method provided by this invention involves the strain converting sodium selenite into nano-selenium, which combines with the secreted PR protein to form nano-selenoprotein. Simultaneously, it synergistically produces polymyxins, achieving a dual effect of immune signal activation and direct bacterial inhibition, solving the problems of weak signaling and lack of synergistic antibacterial effects of traditional inducers.

The invention relates to the technical field of insole preparation, in particular to a sterilization physiotherapy insole and a preparation method thereof. The preparation method comprises the following steps: preparing an upper composite insole layer; preparing a power supply control pad; preparing a lower supporting layer; the upper composite insole layer, the power control layer and the lower supporting layer are sequentially subjected to hot-pressing composition, and the sterilization physiotherapy insole is obtained. And the preparation raw materials of the upper composite insole layer comprise titanium dioxide and a micro ultraviolet LED. In the application, the upper composite insole layer, the power supply control pad and the lower supporting layer are prepared step by step, and then the functional layers are integrated through hot-pressing compounding, titanium dioxide in the upper composite insole layer is matched with a micro ultraviolet LED to play a role in bacteriostasis, and in combination with the synergistic effect of the subsequent functional layers, the functions of sterilization and physical therapy can be integrated; moreover, an irritant antibacterial agent is not needed, the use safety and the health care effect are both considered, and the comprehensive requirements of daily wearing on foot bacterium inhibition and acupuncture point care are met.

The invention discloses a preparation method of plant-source phenolic acid modified bio-based polyurethane foam, and relates to the technical field of polyurethane materials, the preparation method comprises the following steps: dissolving caffeic acid in an organic solvent, mixing with macromolecular polyol, isocyanate, a catalyst and a foaming agent, stirring for foaming, and cooling to room temperature to obtain the plant-source phenolic acid modified bio-based polyurethane foam. After forming and curing, washing and drying to obtain the plant source phenolic acid modified bio-based polyurethane foam. The invention further discloses the plant source phenolic acid modified bio-based polyurethane foam prepared by the preparation method and application of the plant source phenolic acid modified bio-based polyurethane foam in the field of bacterium inhibition. The adopted preparation process is simple and convenient to operate, and the prepared plant source phenolic acid modified bio-based polyurethane foam has the advantages of being natural, environmentally friendly, efficient in bacteriostasis and of a porous structure and shows good application potential in antibacterial adsorption scenes.

The invention discloses a wearable neurosurgical wound nursing dressing and a preparation method thereof. The dressing comprises a flexible fitting layer, a functional dressing layer, a moisture-permeable bacteria-blocking layer and a wearable structure. Adhesive-free attachment is achieved through the bionic micro suction cup array of the flexible attachment layer, and hair damage is avoided; the functional dressing layer contains a drug-loaded hydrogel layer, a nanofiber adsorption layer and a temperature-sensitive color-changing indication layer, and can release repair factors, adsorb exudate and carry out color-changing early warning at the temperature of 34 DEG C or above; the moisture-permeable and bacteria-blocking layer ensures air permeability and bacteria blocking; the annular memory alloy skeleton of the wearable structure radially contracts at 33-36 DEG C, so that the fitness is enhanced, and the annular memory alloy skeleton is matched with the elastic headband to be stably fixed. The dressing can reduce secondary injury and monitor wounds in real time, and is suitable for neurosurgery postoperative care.

The invention discloses a multifunctional emulsion based on natural composite raw materials and a preparation method thereof. The emulsion is of an oil-in-water type, composite particles composed of jackfruit and durian seed inner seed coat particles and tea polyphenol are dispersed in a water phase, and an oil phase can be selected from one or more of n-decane, o-xylene and olive oil. The preparation method comprises the following steps: firstly, obtaining the composite particles, ultrasonically dispersing, standing and diluting, then adding the tea polyphenol, and emulsifying with the oil phase. Experiments show that the emulsion is excellent in performance, the peroxide value is increased by not more than 20% after the emulsion is stored at normal temperature for 3 months, and the oxidation resistance is high; after standing for 1 month at 20-30 DEG C, obvious layering and demulsification are avoided, the oil phase separation rate after centrifugation does not exceed 5%, and the stability is good; the effect of inhibiting common bacteria is outstanding, and the antibacterial property is good. Compared with the prior art, natural raw materials are utilized, the risk of synthesizing an emulsifier is avoided, multi-functionalization is achieved through the synergistic effect of the raw materials, and the preparation method is simple and has wide application prospects.

The invention discloses a bio-based composite fiber skin-friendly fashionable knitted fabric and a weaving method thereof. The fabric is a double-faced fabric with different structures of a surface layer and an inner layer and is formed by weaving biomass collagen composite fiber / modal blended yarn, modal / cotton blended yarn and low-temperature spandex, the structure of the fabric is that variable plain weave and variable twill weave are longitudinally and alternately circulated, the surface layer is in interval continuous twill weave, and the inner layer is in uniform concave point plain weave. A specific stitch length double-sided machine is adopted for weaving, different yarns are threaded according to odd and even paths, and the yarn inlet length and tension of each path are controlled. Through cooperation of functional fibers and a special structure, the fabric has excellent skin-friendly moisture retention, broad-spectrum antibacterial activity (the antibacterial rate is larger than 98%), high air permeability (the air permeability is 820 mm / s) and rich amino acid content (the total content of 16 amino acids accounts for 2.32%), is fashionable and attractive in appearance and is suitable for high-end underwear.

The invention belongs to the technical field of polymer functional materials, and particularly relates to a PPS antibacterial composite fiber membrane and a preparation method thereof.The composite fiber membrane is composed of a PPS matrix membrane, a PDA initiation layer wrapping the outer side of the PPS matrix membrane and an Ag-loaded ZIF functional layer combined with the PDA initiation layer, and the Ag-loaded ZIF functional layer is an Ag-loaded Cu and Zn bimetal ZIF functional layer. The composite fiber membrane has obvious structural characteristics, combines the initiation adhesion layer of PDA, the porous structure of ZIF (Zn / Cu) and the antibacterial property of Ag nanoparticles, and has excellent performance in bacterial inhibition and durability application by relying on the high mechanical strength and chemical stability of the PPS base membrane.

The object of the invention is to provide an improved device for delaying the biological degradation processes of organic materials arranged in a container and a corresponding method. The device according to the invention is designed in such a way that it acts on the interior of a container which serves to hold the organic materials. The device according to the invention comprises at least two of the following components: - a unit for creating a vacuum in the container - a UV radiation emitting unit, - a dewatering unit and / or a drying unit, - a cooling unit,- a fumigation unit for fumigation with germ-inhibiting gases, such as in particular CO2 and / or nitrogen, - a feed unit for adding germ-inhibiting biological substances..

The present invention relates to direct feeding microorganisms for inhibiting Escherichia coli, Salmonella and / or Clostridium in an animal. More specifically, the present invention relates to isolated Bacillus strains CM256 (NRRL number B-67706), CM857 (NRRL number B-67705), CM978 (NRRL number B-67703), CM1004 (NRRL number B-67708) and MDG1607 (NRRL number B-67666), as well as to the use of strains having all of the identifying characteristics of these strains, comprising the above use. The present invention also relates to isolated Bacillus strains CM256 (NRRL number B-67706), CM857 (NRRL number B-67705), CM978 (NRRL number B-67703) and CM1004 (NRRL number B-67708) and the use of strains having all identifying characteristics of these strains in the treatment of plants suffering from a disease caused by E. coli, Salmonella and / or Clostridium.

The invention belongs to the technical field of human body lubricants, and particularly relates to a preservative-free human body lubricant and a preparation method thereof. The lubricant comprises the following components in percentage by mass: 60%-75% of polyol, 5%-10% of betaine, 0.1%-0.5% of hyaluronic acid, 0.5%-2% of a thickening agent and the balance of deionized water. The preparation method comprises the following steps: mixing and stirring under specific conditions; according to the invention, a high osmotic pressure system is constructed by polyol and betaine to inhibit the growth of bacteria, the diameters of inhibition zones on escherichia coli and other bacteria are greater than or equal to 12.0 mm, the moderate and strong antibacterial grade is reached, and a sterile production environment and a special sterilization process are not needed in the preparation process; and compared with the existing preservative-free lubricant, the production period is shortened by more than 25%, and the production cost is reduced by 30-40%.

The invention belongs to the technical field of molecular biology, and particularly relates to a plant immune induced resistance protein EqBPIN1 and application thereof, and the amino acid sequence of the protein EqBPIN1 is shown as SEQ ID No.1. The protein EqBPIN1 provided by the invention can be started from a plant, and the immune system of the plant is enhanced, so that the plant can cope with more diverse environmental changes and infection of various pathogenic bacteria; according to the present invention, the plant disease resistance can be significantly improved, the plant disease resistance can be significantly improved, and the bacterial inhibition effect can be produced when the concentration is 0.1-1 [mu] M, and the plant disease resistance can be significantly improved, such that the plant disease resistance can be significantly improved, and the plant disease resistance can be significantly improved.