25 results about "Bacteriocin" patented technology

A Bifidobacterium longum subsp. infantis YLGB-1496 postbiotic product, a bacteriocin, a preparation method, and a use, specifically relating to the technical field of probiotic postbiotics. The provided probiotic postbiotic product has the following biological functions: enhancing the intestinal barrier function, preventing and / or treating inflammatory bowel diseases, regulating gut flora, relieving intestinal flatulence and resisting oxidation, and has application prospects in preparing health-care foods or drugs for regulating gut flora and resisting oxidation.

The present invention provides a novel resource-recycling technology for converting crustacean waste into violacein. Specifically, an engineered strain of Viola viride that efficiently metabolizes chitin and synthesizes violacein is disclosed. The engineered strain can be used for crustacean waste treatment and chitin resource conversion.

The invention relates to the technical field of rhinitis spray preparation, in particular to a method for preparing allergic rhinitis spray from umbilical cord mesenchymal stem cell exosomes, which comprises the following steps: 1, umbilical cord treatment; 2, culturing umbilical cord mesenchymal stem cells; 3, preparing an umbilical cord mesenchymal stem cell exosome; and 4, preparing the rhinitis spray. The rhinitis spray prepared by the method disclosed by the invention is simpler and more convenient to use. The exosome, the platelet growth factor and other substances in the rhinitis spray well solve the core problems of single curative effect, large side effect, low compliance and the like in the traditional rhinitis treatment, and through the induction and repair functions of the stem cell exosome and the bactericidal ability of the antibacterial peptide, the nasal cavity environment is improved, the anaphylactic reaction is reduced, the nasal mucosa is repaired, and the rhinitis treatment effect is improved. The allergic rhinitis is expected to be fundamentally treated.

A postbiotic product of lactobacillus paracasei K56. The postbiotic product of a probiotic comprises inactivated bacteria of the probiotic and / or metabolites thereof, and the probiotic includes lactobacillus paracasei K56, which has the preservation number of CGMCC No.15139 or DSM27447; the metabolic products comprise: 62.60 mg / 100 g or more of L-methionine and 70.1 mg / 100 g or more of short peptide; and the inactivated bacteria of the probiotic are inactivated bacteria of a probiotic after undergoing fermentation, and the inactivation conditions for the inactivated bacteria involve treating at a temperature ranging from 70°C to 121°C for 5-15 min. The bacteriocin of lactobacillus paracasei K56 comprises exocytotic materials and metabolic products of lactobacillus paracasei K56, and in the bacteriocin, the mass ratio of citric acid to L-methionine is greater than 8:1. The postbiotic product of the probiotic can be applied to intestinal health.

The utility model relates to the field of bacteriocin extraction, and discloses a double-effect lactobacillus bacteriocin extraction device which comprises a tank body, a tank cover is detachably installed on the inner wall of the upper end of the tank body, a motor is fixedly connected to the outer wall of the upper end of the tank cover, and a rotating shaft penetrates through and is rotationally connected to the inner wall of the bottom end of the tank cover; a spiral blade is fixedly connected to the outer wall of the rotating shaft, a stirring plate is fixedly connected to the outer wall of the rotating shaft, and a spoiler is fixedly connected to the inner wall of the tank body. According to the double-effect lactobacillus bacteriocin stirring device, the rotating shaft is driven to rotate through the operation of the motor, and the rotating shaft drives the spiral blade to rotate to lift the extraction liquid on the lower side upwards and drives the stirring plate to rotate to stir the extraction liquid and double-effect lactobacillus bacteriocin raw materials in the tank body; the double-effect lactic acid bacteria bacteriocin raw material can be more fully mixed with the extract liquor through the spoiler of the rotating extract liquor and double-effect lactic acid bacteria bacteriocin raw material mixture, and the mixing effect is good.

The invention discloses a private care wet tissue containing a bacteriostatic composition, the private care wet tissue comprises a composite non-woven fabric base material and an impregnation liquid, the impregnation liquid comprises the bacteriostatic composition, a soothing anti-inflammatory agent, a moisturizing repairing agent, an odor removal regulator, a cosolvent and a stabilizer; the preparation method comprises the following steps: adding purified water, a citric acid-sodium citrate buffer agent and EDTA (Ethylene Diamine Tetraacetic Acid) disodium into a vacuum stirring tank, then adding glycerol, micromolecular sodium hyaluronate and ceramide 3, and then adding L-lactic acid and a lactobacillus acidophilus fermentation product; sequentially adding nisin, cranberry extract, polyhexamethylene biguanide hydrochloride and beta-cyclodextrin inclusion tea tree essential oil, and finally adding dipotassium glycyrrhizinate, allantoin and citrus extract. Different from the prior art that private care wet tissues only have a single antibacterial function and neglect flora balance, peculiar smell is covered by depending on essence, the base material and the steeping liquor are designed at the same time, and the functions of bacteriostasis and antibiosis, gentle private care, odor removal and itching relieving, inflammation prevention and swelling prevention, flora balance maintenance and the like are achieved.

The application discloses a gelatin-bacteriocin amino acid-dialdehyde starch composite film and a preparation method and application thereof. The preparation method of the composite film comprises the following steps: adding a crosslinking agent into a gelatin solution, adding N-hydroxysuccinimide into the gelatin solution after activation for a set time, then adding bacteriocin amino acid into the gelatin solution, coupling for a set time under weak acid condition and in the dark, and obtaining Gel-MAAs; dissolving the Gel-MAAs in water to obtain a composite solution; dissolving dialdehyde starch in water, gelatinizing, adjusting to weak alkalinity, and obtaining a dialdehyde starch solution; adding the dialdehyde starch solution into the composite solution, mixing uniformly, reacting at 35-45 DEG C for 1-3 h, adding glycerol with a mass fraction of 1-5%, ultrasonic degassing, pouring the film solution into a mold, and drying, and thus the composite film is obtained. The composite film has excellent mechanical properties, physicochemical properties, ultraviolet resistance, good biocompatibility, and great potential in the field of active composite films.

The present invention relates to nuclease bacteriocin-derived antimicrobial protein complexes, their preparation and use, and related products having antimicrobial surfaces. The complexes comprise a nuclease bacteriocin polypeptide and an immunity polypeptide linked to a bacteria-binding moiety.

The weissella cibarius is named as weissella cibarius H3 and is preserved in the Guangdong Microbial Culture Collection Center, the preservation number of the weissella cibarius H3 is GDMCC NO.65902, the preservation address of the weissella cibarius H3 is the 5th floor of the building 59, No. 100 courtyard, Xianlie Middle Road, Guangzhou, and the preservation date of the weissella cibarius H3 is February 18, 2025. The Weissella sinus producing bacteriocin disclosed by the invention can produce bacteriocin with broad-spectrum antibacterial activity, and the bacteriocin has an antibacterial effect on various food-borne gram-negative pathogenic bacteria (vibrio alginolyticus, vibrio parahaemolyticus and the like) and gram-positive bacteria (listeria monocytogenes, enterococcus faecalis and the like), and has application potential in the aspects of food preservation and fresh keeping.

The application belongs to the field of biological medicine, and particularly relates to an information bacteriocin array against small cell lung cancer and application thereof. Amino acid sequences such as SEQ ID NO: 1-30 are provided. A drug against small cell lung cancer is also provided, which comprises a fusion protein obtained by connecting a channel domain of colicin with polypeptides with amino acid sequences such as SEQ ID NO: 1-30. Pharmacodynamics experiments prove that in each growth cycle, a mouse tumor-bearing model cannot escape the recognition and killing of the information bacteriocin array composed of thirty kinds of fusion proteins. Guinea pig model experiments prove that the continuous use of the information bacteriocin array for 30 days does not produce any toxic side effects in the animal model. The information bacteriocin array can be used as an effective drug for treating small cell lung cancer.

The invention relates to an aquatic product source Weissella cibaria, the Weissella cibaria is named Weissella cibaria Gut 2, and is preserved in the Guangdong Microbial Culture Collection Center, the preservation number is GDMCC NO.65901, the preservation address is the 5th floor, No. 59 building, No. 100 Courtyard, Xianlie Middle Road, Guangzhou, and the preservation date is February 18, 2025. The aquatic product source Weissella cibaria is named as Weissella cibaria Gut 2, and the Weissella cibaria is preserved in the Guangdong Microbial Culture Collection Center, the preservation number is GDMCC NO.65901. The aquatic product source Weissella cibaria can generate bacteriocin with broad-spectrum antibacterial activity, the bacteriocin has a remarkable inhibition effect on various food-borne pathogenic bacteria, due to the characteristics, the aquatic product source Weissella cibaria can become a potential antibiotic substitute for controlling the food-borne pathogenic bacteria, and a promising application scheme is provided for prolonging the shelf life of food.

Disclosed are Bifidobacterium lactis BL-99 bacteriocin, inactivated bacteria, a preparation method, and a use thereof. The Bifidobacterium lactis BL-99 bacteriocin comprises the exocytotic substances and metabolic products of Bifidobacterium lactis BL-99, the mass ratio of citric acid to L-methionine in the bacteriocin is greater than 2:1, and the preservation number of the Bifidobacterium lactis BL-99 is CGMCC No.15650. The Bifidobacterium lactis BL-99 bacteriocin has antioxidant functions and can be applied in food and pharmaceuticals. Moreover, compared with mixtures comprising whole dead cells + cell wall components + cell membrane components + cell-free supernatant, the bacteriocin is a liquid or water-soluble powder, and offers additional advantages for use in certain liquid beverages and pharmaceuticals.

The invention discloses bifidobacterium lactis BL-99 bacteriocin as well as a preparation method and antioxidant application thereof, the bifidobacterium lactis BL-99 bacteriocin comprises exocytosis of the bifidobacterium lactis BL-99 and metabolite of the bifidobacterium lactis BL-99, the mass ratio of citric acid to L-methionine in the bacteriocin is greater than 2: 1, and the preservation number of the bifidobacterium lactis BL-99 is CGMCC (China General Microbiological Culture Collection Center) No.15650. The bifidobacterium lactis BL-99 bacteriocin disclosed by the invention has an antioxidant function, can be applied to foods and medicines, and compared with a mixture comprising complete dead cells, cell wall components, cell membrane components and acellular supernatant, the bacteriocin disclosed by the invention is liquid or water-soluble powder, and has more advantages when being used in some liquid beverages and medicines.

The invention discloses bifidobacterium longum subsp. Infantis YLGB-1496 bacteriocin as well as a preparation method and antioxidant application thereof, the bifidobacterium longum subsp. Infantis YLGB-1496 bacteriocin comprises exocytosis of the bifidobacterium longum subsp. Infantis YLGB-1496 and metabolite of the bifidobacterium longum subsp. Infantis YLGB-1496, the mass ratio of citric acid to L-methionine in the bacteriocin is greater than 25: 1, and the mass ratio of citric acid to L-methionine is greater than 1: 1. The preservation number of the bifidobacterium longum subsp. Infantis YLGB-1496 is CCTCC (China Center For Type Culture Collection) No.M2011122. The bifidobacterium longum subsp The bifidobacterium longum subsp. Infantis YLGB-1496 bacteriocin disclosed by the invention has an antioxidant function, can be applied to foods and medicines, and compared with a mixture comprising complete dead cells, cell wall components, cell membrane components and cell-free supernatant, the bacteriocin disclosed by the invention is liquid or water-soluble powder, and has more advantages when being used in some liquid beverages and medicines.

The invention discloses lactobacillus paracasei K56 bacteriocin as well as a preparation method and antioxidant application thereof, the lactobacillus paracasei K56 bacteriocin comprises exosomes of lactobacillus paracasei K56 and metabolites of lactobacillus paracasei K56, the mass ratio of citric acid to L-methionine in the bacteriocin is greater than 8: 1, and the preservation number of the lactobacillus paracasei K56 is CGMCC No.15139. The lactobacillus paracasei K56 bacteriocin has the advantages that the lactobacillus paracasei K56 bacteriocin can be used for preparing the lactobacillus paracasei K56 bacteriocin; the lactobacillus paracasei K56 bacteriocin disclosed by the invention has an antioxidant function, can be applied to foods and medicines, and compared with a mixture comprising complete dead cells, cell wall components, cell membrane components and acellular supernatant, the bacteriocin disclosed by the invention is liquid or water-soluble powder, and has more advantages when being used in some liquid beverages and medicines.

The application belongs to the technical field of microbial fermentation, and provides Lactobacillus paracasei subsp. paracasei with bacteriostatic and fermentation improvement characteristics, as well as a bacteriocin and application thereof. The classification name of the Lactobacillus paracasei subsp. paracasei is Lactobacillus paracasei subsp. paracasei, which has been preserved in the Guangdong Microbial Culture Collection Center (GDMCC) on June 25, 2025, the address of the preservation center is Building 59, 5th Floor, 100, Martyrs' Road, Guangzhou, and the preservation number is GDMCC No: 66588. The YCL29 bacteriocin produced by the application exhibits a significant inhibitory effect on Vibrio parahaemolyticus, and the starter prepared by the strain can be used to improve the flavor quality and metabolic regulation of fermented fish tea, thereby providing a new solution for developing a local starter with the functions of inhibiting raw material source pathogenic bacteria and regulating fermentation quality.

The invention relates to a Weissella cibaria strain with narrow-spectrum antibacterial activity, the Weissella cibaria strain is named Weissella cibaria B4, and is preserved in the Guangdong Microbiological Culture Collection Center, the preservation number is GDMCC NO.65903, the preservation address is the 5th floor, No. 59 building, No. 100 Courtyard, Xianlie Middle Road, Guangzhou, and the preservation date is February 18, 2025. The Weissella cibaria B4 can generate narrow-spectrum bacteriocin, has a remarkable bacteriostatic effect on various food-borne gram-negative pathogenic bacteria, but has no bacteriostatic effect on most gram-positive bacteria, so that the Weissella cibaria B4 can be used for targeted inhibition of growth of specific pathogenic bacteria, and has a broad application prospect. The method has good application value in the fields of biological medicine and food safety.

The present disclosure provides an antimicrobial composition as well as a process using same to limit microbial activity in a yeast medium. The antimicrobial composition includes at least one weak acid, optionally in combination with an acid stable bacteriocin and / or an antibiotic. The antimicrobial composition can be used for propagating yeasts and for making a fermentation product. The present disclosure also provides yeasts and lactic acid bacteria that can be used with the antimicrobial composition.

The present invention relates to the field of medicine, specifically the field of bacterial infection and treatment thereof.

The present invention relates to protein bacteriocins (PBs) as therapeutic agents, specifically protein complexes comprising two or more PB molecules associated with a protein scaffold, wherein the protein scaffold comprises a cognate immunity protein domain for each PB effector moiety. In particular, the present invention provides an antibacterial protein complex comprising: (a) a first PB molecule and a second PB molecule; and (b) an immunity protein scaffold comprising a first immunity protein domain and a second immunity protein domain, wherein the first and second immunity protein domains are non-covalently bound to the first and second PB molecules, respectively.

The present application relates to the technical field of food chemistry processing, and discloses a ginseng fruit juice drink with high clarity and long shelf life and a preparation method thereof, wherein the preparation method comprises the following steps: subjecting fresh ginseng fruits to blanching color protection and acidification pretreatment to obtain pretreated ginseng fruit juice; subjecting free phenolic substances in the fruit juice to oxidative polymerization under the condition of continuous oxygen supply with laccase as a catalyst, removing insoluble polyphenol polymers by centrifugation to obtain depolymerized ginseng fruit juice; preparing TEMPO oxidized nanocellulose with a surface rich in carboxyl groups; adding the above nanocellulose dispersion, honey and streptococcal bacteriocin into the depolymerized fruit juice, and performing membrane filtration to obtain stabilized ginseng fruit juice; performing pasteurization, filling into a packaging container with a composite oxygen barrier inner liner of sodium alginate and beeswax, and cold storage aging to obtain a finished product.

The invention discloses lactobacillus paracasei ET-22 bacteriocin as well as a preparation method and antioxidant application thereof, the lactobacillus paracasei ET-22 bacteriocin comprises exocytosis of lactobacillus paracasei ET-22 and metabolite of lactobacillus paracasei ET-22, the mass ratio of citric acid to L-methionine in the bacteriocin is greater than 15: 1, and the mass ratio of citric acid to L-methionine in the bacteriocin is greater than 15: 1. The preservation number of the lactobacillus paracasei ET-22 is CGMCC (China General Microbiological Culture Collection Center) No. 15077. The lactobacillus paracasei ET-22 bacteriocin disclosed by the invention has an antioxidant function, can be applied to foods and medicines, and compared with a mixture comprising complete dead cells, cell wall components, cell membrane components and acellular supernatant, the bacteriocin disclosed by the invention is liquid or water-soluble powder, and has more advantages when being used in some liquid beverages and medicines.

The present invention relates to protein bacteriocins (PBs) as therapeutic agents, and in particular to protein complexes comprising two or more PB molecules associated with a protein scaffold comprising a homologous immune protein domain against an effector portion of the corresponding PB. In particular, the present invention provides an antibacterial protein complex comprising: (a) a first PB molecule and a second PB molecule; and (b) an immune protein scaffold comprising a first immune protein domain and a second immune protein domain; wherein the first immune protein domain and the second immune protein domain are non-covalently bound to the first PB molecule and the second PB molecule, respectively.

The invention provides a plant source anti-corrosion compound as well as a preparation method and application of the plant source anti-corrosion compound. The feed additive is prepared from the following components in parts by weight: 3 to 5 parts of clove extract, 3 to 5 parts of rosemary extract, 2 to 4 parts of star anise extract, 0.2 to 0.5 part of nisin, 0.1 to 0.3 part of natamycin, 0.1 to 0.5 part of epsilon-polylysine, 0.1 to 0.5 part of vitamin E, 0.1 to 0.5 part of sodium alginate, 1 to 2 parts of chitosan, 5 to 10 parts of maltodextrin and 2 to 4 parts of starch octenyl succinate. The double-wall material microencapsulated plant source preservative compound disclosed by the invention is used as a preservative, under the addition amount of 0.3 wt%, the normal-temperature shelf life of baked food can be prolonged from 3 days to 9-12 days, and the inhibition rate for aspergillus niger, aspergillus flavus, saccharomyces cerevisiae and bacillus subtilis is gt; the quality and the flavor are not influenced; the three major pain points of thermal stability, flavor compatibility and antibacterial broad spectrum are synchronously solved; the process is simple and wide in application prospect.