The invention discloses a preparation method and application of a pomelo-
flavor tobacco extract, and the preparation method of the pomelo-
flavor tobacco extract comprises the following steps: mixing and dissolving neoxanthine and Tween 80 in
dichloromethane, and adding absolute ethyl
alcohol after reduced pressure
distillation;
grinding tobacco leaves into tobacco
powder, mixing the tobacco
powder with water for sterilization, and adding a neoxanthine solution;
klebsiella variicola HNYJ-3 and
enterobacter HNYJ-4 are inoculated into a
liquid culture medium to culture seed solutions, the two seed solutions are mixed and inoculated into a
mixed solution of tobacco and neoxanthine, and shaking culture
fermentation is performed; after
fermentation, centrifugal extraction and rotary
evaporation are performed, absolute ethyl
alcohol and
propylene glycol are added, and the pomelo-
flavor tobacco extract is obtained. The two strains are used for fermenting a mixture of neoxanthine and tobacco to generate noottanone, alpha-
truffle alcohol, perillone,
carvone, dihydrocarvone and other
aroma components, and when the two strains are applied to cigarette products, the
aroma quality and the
aroma amount of
smoke can be improved, miscellaneous gas is reduced, and the permeability is improved.