5 results about "Limonium" patented technology

The invention discloses a limonium bicolor gene LbZFP8 and application thereof, and relates to the technical field of biological genetic engineering. The invention relates to a limonium bicolor gene LbZFP8 and application thereof. The limonium bicolor gene LbZFP8 is a gene for coding a protein composed of an amino acid sequence as shown in SEQ ID NO: 1; the biological material is recombinant DNA, an expression cassette, a transposon, a plasmid vector, a virus vector or engineering bacteria. The gene provided by the invention can significantly improve the salt tolerance and oxidation resistance of a target plant by transforming plant cells. Experiments show that under the salt stress condition, the survival rate of the LbZFP8 overexpressed arabidopsis thaliana plant is increased by about 45% compared with that of a control group. Meanwhile, the compound plays a key role in regulating and controlling an active oxygen scavenging system, and the activity of SOD and POD enzymes can be effectively enhanced. The technology provides an important gene resource for cultivating new varieties of crops with strong stress resistance, and has a wide application prospect.

The invention relates to the technical field of plant genetic engineering, and discloses a limonium bicolor gene LbYAB1 and application thereof. The new function of the LbYAB1 is found, firstly, the expression of the LbYAB1 in salt glands and leaf primordia is proved through RNA in-situ hybridization and a promoter driven GUS tissue staining technology, and the expression of the LbYAB1 is induced by treatment of 6-benzylaminopurine. Secondly, a genetic transformation technology is updated, a stable overexpressed LbYAB1 strain is obtained, and by exploring the LbYAB1 function of an overexpressed and silent strain, it is found that compared with a wild type, development of salt glands of the overexpressed strain is inhibited, the salt secreting capacity is weakened, and the salt tolerance is reduced; a silent strain shows an opposite phenotype, and a result proves that the LbYAB1 is a negative regulation factor for salt gland development. Overexpression of the gene in limonium bicolor and arabidopsis thaliana both leads to enhancement of salt sensitivity. The invention not only lays a foundation for further exploring and revealing the development mechanism of salt glands, but also opens up a new path for cultivating salt-tolerant crops.

The application discloses a culture medium for tissue culture of Limosella australis, which comprises a primary culture medium, a proliferation and differentiation culture medium and a rooting culture medium. The primary culture medium is MS+6BA 0.4-0.6 mg / L+NAA 0.04-0.06 mg / L+AC 0.8-1.2 mg / L+2,4-D 0.04-0.06 mg / L. The proliferation and differentiation culture medium is modified MS+6BA 0.09-0.11 mg / L+NAA 0.04-0.06 mg / L+AC 1.5-2.5 mg / L. The rooting culture medium is 1 / 2MS+IAA 0.09-0.11 mg / L+AC 2.5-3.5 mg / L. The technical scheme has the highest callus induction rate of 86.7%, the highest differentiation rate of 86.7% and the highest rooting rate of 93.3%.

This invention relates to the field of plant genetic engineering, specifically to the LbMYB75 gene of *Limonium bicolor* and its applications. This invention discloses that the transcription factor LbMYB75 is specifically highly expressed in the early stages of salt gland development and is significantly induced by salt. This invention confirms that the LbMYB75 gene positively regulates salt gland development and salt secretion in *Limonium bicolor*. Salt gland density is significantly increased in *Limonium bicolor* lines overexpressing the LbMYB75 gene, while salt gland density is significantly decreased in LbMYB75 mutant lines. The transcription factor LbMYB75 positively regulates the salt tolerance of *Limonium bicolor*; compared with the wild type, the overexpressing lines under high salt conditions show higher levels of malondialdehyde (MDA) and sodium. + and Na + / K + K decreased significantly. + Increased proline; This invention elucidates the regulatory role of the LbMYB75 transcription factor in salt gland development of *Limonium bicolor*, providing genetic resources and theoretical basis for improving the salt tolerance of non-halophytes through transgenic means, and contributing to the development and utilization of saline-alkali soils.

This invention relates to the field of plant tissue culture technology, specifically a method for culturing *Limonium bicolor* tissue, comprising five steps: explant preparation and sterilization, callus induction culture, adventitious shoot proliferation culture, rooting culture, and hardening-off transplanting. Modified polyvinylpyrrolidone (PVP) and modified chitosan form a composite material through borate ester bonds, which accumulates around the explant. MES maintains pH stability, allowing PPVP to continuously diffuse into the wound. Modified chitosan blocks oxygen from direct contact with the wound surface, protecting endogenous phenols; its catechol groups chelate the PPO cofactor Cu. 2+ Activated carbon adsorbs free phenolic substrates in the culture medium, reducing quinone formation at its source. Modified polyvinylpyrrolidone inhibits PPO catalytic activity; grafted ascorbic acid can reduce already formed o-quinones back to catechol, directly removing brown pigment precursors.