41 results about "Isatis" patented technology

The invention relates to a traditional Chinese medicine composition for cooling blood, removing dampness and relieving itching and a preparation method and application thereof, and belongs to the technical field of traditional Chinese medicine. The invention provides a traditional Chinese medicine composition for cooling blood, removing dampness and relieving itching. The traditional Chinese medicine composition is prepared from the following traditional Chinese medicine raw materials: herba portulacae, radix rehmanniae recen, folium isatidis, radix sophorae flavescentis, fructus kochiae, cortex phellodendri, pericarpium zanthoxyli, schizonepeta spike, gallnut and flos chrysanthemi. According to the invention, radix rehmanniae recen and pepper are used as monarch drugs, purslane, folium isatidis and schizonepeta spike are used as ministerial drugs, and radix sophorae flavescentis, fructus kochiae, cortex phellodendri, gallnut and chrysanthemum are used as adjuvant drugs. The monarch drug radix rehmanniae is cold in nature and has the effects of removing heat to cool blood and nourishing yin to promote salivation, the pepper is warm in nature and killing parasites to relieve itching, and the radix rehmanniae and the pepper are cold and warm in nature and are mutually used, so that the efficacy of the drug is not overheated or overcold, and the composition conforms to the physique characteristics of young yin and young yang of infants; ministerial drugs including purslane, folium isatidis and schizonepeta spike have the effects of removing heat, cooling blood, detoxifying, dispelling wind, arresting itching and enhancing the effect of the monarch drugs; the adjuvant drugs such as radix sophorae flavescentis, fructus kochiae, cortex phellodendri, gallnut and chrysanthemum have the effects of clearing heat, removing dampness, detoxifying, astringing, dispelling wind and arresting itching, and assists the monarch and ministerial drugs to enhance the efficacy.

The invention relates to a cultivation method of an overexpressed EVM0003075 gene isatis tinctoria plant, and belongs to the technical field of plant cultivation. The method comprises the following steps: firstly, transforming an agrobacterium strain through a constructed agrobacterium transformation vector for expressing the isatis tinctoria EVM0003075 gene, then transforming an explant of the isatis tinctoria by using a screened recombinant agrobacterium strain, regenerating a seedling of the isatis tinctoria, and screening and culturing the seedling of the isatis tinctoria with stable overexpression of the EVM0003075 gene, so as to obtain an isatis tinctoria plant with increased isatin content in the isatis tinctoria. The expression level of the EVM0003075 gene of the transgenic isatis tinctoria is improved to 5-9 times, the content of the isatis tinctoria is remarkably improved, and the character that the accumulation period of the isatis tinctoria is short is shown. By adopting the method disclosed by the invention, the content of the isatin in the isatis tinctoria can be increased to 10-66 times. The specific information content of the patent of the method for improving the content of the isatin in the isatis tinctoria through overexpression of the EVM0003075 gene is shown in the specification.

The invention discloses a traditional Chinese medicine composition for treating verruca plana and a preparation method and application thereof, and the traditional Chinese medicine composition for treating verruca plana comprises the following components in parts by weight: 48-72 parts of isatis root, 48-72 parts of coix seed, 48-72 parts of purslane, 24-36 parts of lithospermum, 16-24 parts of peach kernel, 16-24 parts of safflower, 48-72 parts of horsetail, 16-24 parts of rhizoma cyperi, 48-72 parts of nacre mother of pearl, 16-24 parts of honeycomb and 48-72 parts of raw oyster. The nacre mother and the nidus vespae with high dosage are combined with the raw oysters, so that double enhancement of hardness softening, stagnation eliminating, toxic material counteracting and virus resisting is realized, the combination of the nacre mother and the nidus vespae is not seen in the prior art, and the cure rate can be remarkably increased and the recurrence rate can be remarkably reduced.

The invention discloses a traditional Chinese medicine composition for treating sepsis as well as a preparation method and application of the traditional Chinese medicine composition. The traditional Chinese medicine composition is prepared from 1-15 parts of sulfur, 3-25 parts of calamitas urinae hominis, 1-20 parts of wine-treated rhubarb, 10-30 parts of fructus cannabis, 10-50 parts of radix bupleuri, 5-30 parts of scutellaria baicalensis, 5-25 parts of folium isatidis, 5-25 parts of peach kernels, 5-30 parts of salvia miltiorrhiza, 5-30 parts of angelica sinensis, 5-30 parts of sophora flower charcoal, 5-25 parts of fructus gardeniae praeparatus and 5-60 parts of wine-treated fructus corni. According to the invention, through a combined mode of'monarch drug targeted oral administration and synergistic decoction of minister, assistant and guide drugs', the whole-course intervention of'upstream inhibition, midstream blocking and downstream regulation 'on sepsis immune disorder can be realized, meanwhile, the functions of warming yang, dispelling cold, cooling blood and detoxifying are taken into account, and the pro-inflammatory / anti-inflammatory balance is successfully recovered to a physiological state; the action mode is superior to that of a traditional single anti-inflammatory strategy and a traditional Chinese medicine application mode of whole-prescription decoction. In a standard animal model, the traditional Chinese medicine composition definitely shows the dose-dependent extremely remarkable effects of anti-inflammation, immunoregulation, multi-organ protection and overall condition improvement, the curative effect of a high-dose group of the traditional Chinese medicine composition is equivalent to that of a classic potent medicine, and the traditional Chinese medicine composition has more advantages in the aspects of recovering immune homeostasis, warming yang and strengthening the body resistance.

The invention discloses a compound traditional Chinese medicine formula for treating viral infection and a production method of the compound traditional Chinese medicine formula. 5-10 parts of radix isatidis; 5-15 parts of coix seeds; 3-6 parts of liquorice; 5-15 parts of poria cocos; 1-3 parts of mung beans; 5-10 parts of tremella fuciformis; 3-10 parts of quercetin; and 5-15 parts of carambola. The invention also discloses a preparation method of the compound traditional Chinese medicine oral liquid, capsules, granules and tablets. The medicines are combined, and clinical tests show that the medicine has the effects of clearing away heat and toxic materials, tonifying spleen and promoting diuresis, protecting liver and kidney, killing viruses and the like. The traditional Chinese medicine is suitable for cold caused by various viruses and has the effects of relieving cough, abating fever, diminishing inflammation and the like.

This invention discloses a compound traditional Chinese medicine powder inhaler for respiratory tract infections and its preparation method, relating to the field of pharmaceutical formulation technology. The compound traditional Chinese medicine powder inhaler for respiratory tract infections is composed of polysaccharide composite gel microspheres, forsythia, scutellaria, honeysuckle, and isatis root. The polysaccharide composite gel microspheres are composed of seaweed sulfated polysaccharide, lentinan, calcium chloride, and curcumin. Seaweed sulfated polysaccharide, lentinan, curcumin, and calcium chloride cross-link to form a three-dimensional network spherical structure, improving the particle morphology and flowability of the micropowder. Furthermore, due to its good hygroscopic and moisturizing properties, it competitively reduces the moisture absorption of the micropowder, preventing aggregation and further improving flowability. In addition, the gel microspheres have excellent adhesion, adhering to the infection site after entering the respiratory tract, reducing expulsion losses due to respiration, maintaining an effective drug concentration at the infection site, and improving therapeutic efficacy.

The invention discloses a traditional Chinese medicine composition for treating acute pharyngitis as well as a preparation method and application thereof, and belongs to the technical field of traditional Chinese medicines. The technical problems to be solved are that the traditional Chinese medicine composition for treating acute pharyngitis is not suitable for long-term administration, is not suitable for sensitive people and is easy to damage spleen and stomach. The invention provides a traditional Chinese medicine composition for treating acute pharyngitis. The traditional Chinese medicine composition is prepared from the following raw materials: honeysuckle flower, fructus forsythiae, radix isatidis, herba menthae, radix bupleuri, fructus arctii, schizonepeta spike, gypsum, radix scutellariae, fructus gardeniae, radix platycodonis, radix paeoniae rubra, rhizoma phragmitis, semen armeniacae amarae, herba lophatheri, fructus aurantii, radix saposhnikoviae, medicated leaven, stiff silkworm and liquorice root. The traditional Chinese medicine composition disclosed by the invention can inhibit inflammatory reaction by inhibiting phosphorylation levels of PI3K / AKT and RAS / MAPK signal pathways AKT and MAPK, so that acute pharyngitis is improved.

The invention provides a traditional Chinese medicine composition for improving the survival rate of piglets and a preparation method and application thereof, and belongs to the technical field of feed additives. The traditional Chinese medicine composition is prepared from the following raw materials in parts by weight: 6 to 10 parts of fructus forsythiae, 15 to 22 parts of radix isatidis, 15 to 22 parts of radix scutellariae, 8 to 12 parts of honeysuckle flower, 5 to 8 parts of herba taraxaci, 10 to 15 parts of rhizoma atractylodis macrocephalae, 8 to 12 parts of poria cocos, 6 to 10 parts of rhizoma dioscoreae, 12 to 18 parts of radix astragali seu hedysari, 8 to 12 parts of radix bupleuri, 5 to 8 parts of liquorice root and 3 to 6 parts of semen ziziphi spinosae. Based on the traditional Chinese medicine theory of supporting healthy energy and giving consideration to both symptoms and root causes, a three-dimensional synergistic system of clearing heat, resisting bacteria, invigorating spleen, benefiting intestines, tonifying qi and resisting stress is constructed to adapt to the physiological characteristics of weaned piglets, so that the problems of low survival rate, slow growth and substandard quality in weaned piglet breeding are solved, and the survival rate of the weaned piglets is remarkably improved.

The invention discloses an isatis root compound preparation and a method for preventing and controlling tomato mosaic virus, and relates to the technical field of agricultural planting, the isatis root compound preparation is prepared from the following raw materials in parts by weight: 15-20 parts of isatis root, 15-20 parts of radix sophorae flavescentis, 10-15 parts of scutellaria baicalensis, 10-15 parts of szechwan chinaberry fruit, 8-12 parts of radix bupleuri, 8-12 parts of rheum officinale, 8-12 parts of astragalus membranaceus, 8-12 parts of radix stemonae, 5-8 parts of fructus forsythiae and 5-8 parts of saponin. The preparation method comprises the following steps: weighing the traditional Chinese medicine decoction pieces, fully mixing and crushing; adding clear water according to a mass ratio, uniformly stirring, heating and decocting; cooling, and adding a microbial agent for fermentation; according to the method, the traditional Chinese medicine compound preparation with the radix isatidis and the radix sophorae flavescentis as monarch drugs is adopted, the tomato mosaic virus is prevented and controlled in a green mode, the occurrence of virus diseases in the tomato growth period can be effectively prevented and controlled, and chemical pesticides are reduced or not used. And the traditional Chinese medicines are used for replacing chemical pesticides, so that the method is green and safe, no chemical pesticide is used in the whole tomato production process, and real zero pesticide residue is realized.

The invention discloses a composition containing an astragalus extract as well as a preparation method and application of the composition. The composition comprises a carrier layer, a core functional layer, an assistant functional layer, a functional powder layer and a probiotic layer from inside to outside. According to the invention, monarch and ministerial drugs (astragalus membranaceus, codonopsis pilosula, bighead atractylodes rhizome and poria cocos) are taken as a core layer through a fluidized bed, adjuvant and conductant drugs (isatis root, dandelion and platycodon grandiflorum) are taken as a secondary layer, sequence batch layering is realized, and directional release can be realized.

1. Name of the product in this design: Packaging Box (Isatis Root Tea). 2. Purpose of this design: For packaging pharmaceuticals. 3. The key design features of this product are the combination of shape and pattern. 4. The image or photograph that best illustrates the design's key points: a 3D model.

The invention discloses a crocodile functional compound feed and a preparation method thereof, and the crocodile functional compound feed comprises the following raw materials: a compound animal protein source, a compound vegetable protein and energy source, a core functional additive, a compound grease source, a compound premix and a phosphorus calcium source, the functional additive comprises astragalus membranaceus, isatis root, radix bupleuri, liquorice, oriental wormwood, cowherb seed, astaxanthin, nano-selenium and zinc methionine, the compound premix comprises vitamin and mineral components, and the bait coefficient of the compound feed is lower than 1.5. According to the feed, chilled fresh fish can be replaced with the efficient replacement ratio of 1: 5, the breakthrough breeding effects that the survival rate of crocodiles is increased by 30%, the growth rate is increased by 20%, the laying rate is increased by 40%, and the bait coefficient is reduced to 1.3 or below are achieved, and the economic benefits are extremely remarkable.

The invention discloses a compound traditional Chinese medicinal material feed additive for stress conditioning of calves as well as preparation and application of the compound traditional Chinese medicinal material feed additive. Comprising the following raw materials: 1-3 parts of schizonepeta, 1-3 parts of cassia twig, 3-9 parts of ephedra, 2-6 parts of coptis chinensis, 1-3 parts of scutellaria baicalensis, 3-9 parts of cape jasmine, 1-3 parts of poria cocos, 5-15 parts of astragalus membranaceus, 1-3 parts of bighead atractylodes rhizome, 5-15 parts of platycodon grandiflorum, 5-15 parts of radix stemonae, 3-9 parts of white mulberry root-bark, 3-9 parts of hawthorn, 3-9 parts of massa medicata fermentata fujianensis, 5-15 parts of malt, 4-12 parts of radix bupleuri, 3-9 parts of mangnolia officinalis, 2-6 parts of semen lepidii, 1-3 parts of fructus forsythiae, 3-9 parts of ligusticum wallichii, 1-3 parts of houttuynia cordata Through the synergistic effect of multiple traditional Chinese medicines, the stress of the calves can be relieved from multiple dimensions of resisting viruses, regulating intestinal flora, enhancing immunity and the like.

The invention belongs to the technical field of veterinary traditional Chinese medicine preparations, and particularly relates to a veterinary traditional Chinese medicine compound aerosol inhalation preparation which is prepared from traditional Chinese medicines with the effects of clearing away heat and toxic materials, ventilating the lung, relieving cough, relieving sore throat and diminishing swelling as raw materials through scientific compatibility and a specific process. The raw material medicines comprise, by weight, 15-30 parts of honeysuckle, 12-25 parts of fructus forsythiae, 10-20 parts of radix isatidis, 8-18 parts of platycodon grandiflorum, 6-15 parts of mint, 5-12 parts of liquorice, 6-14 parts of almond, 8-16 parts of folium mori, 10-22 parts of chrysanthemum and 8-18 parts of scutellaria baicalensis; the requirements of the raw material medicines are as follows: the honeysuckle is a dry flower bud or a flower with initial blooming of a honeysuckle plant, and is dried at low temperature, and the water content is 8-12%; the traditional Chinese medicine composition is prepared from traditional Chinese medicines with the effects of clearing away heat and toxic materials, ventilating the lung, relieving cough, relieving sore throat and diminishing swelling as raw materials through scientific compatibility and a specific process, has the advantages of high targeting property, quick response, definite curative effect, small toxic and side effects, difficulty in drug resistance generation and the like, is excellent in atomization performance, is suitable for group administration, and can effectively solve the problem of prevention and treatment of respiratory diseases of livestock and poultry.

The invention discloses a traditional Chinese medicine composition for preventing and treating chikungunya fever as well as a preparation method and application of the traditional Chinese medicine composition. The composition is prepared from sweet wormwood herb, honeysuckle flower, giant knotweed rhizome, coix seed, red paeony root and liquorice root according to a specific ratio, and has the effects of clearing away heat and toxic materials, eliminating dampness and promoting superficies, and promoting blood circulation to remove meridian obstruction on the basis of the theory of'warm pathology '. Experiments show that the composition can significantly inhibit chikungunya virus replication (EC = 47.2 [mu] g / mL), has better effects than ribavirin and isatis root, and can effectively relieve fever and arthralgia. The invention also provides a plurality of dosage forms such as decoction, granules and spray, is suitable for large-scale prevention and clinical treatment of epidemic areas, and has the advantages of multi-link effectiveness, low cost and small side effect.

This invention discloses a method for determining the content of a traditional Chinese medicine compound containing honeysuckle, isatis root, and scutellaria. The method includes the following steps: taking a test solution of the traditional Chinese medicine compound, a first reference solution, and a second reference solution for detection. The chromatographic conditions are as follows: using a chromatographic column packed with octadecyl-bonded silica gel; mobile phase A selected from one or more of acetonitrile, methanol, and tetrahydrofuran; and mobile phase B being an acidic aqueous solution, an alkaline aqueous solution, and / or a buffer salt aqueous solution. The traditional Chinese medicine compound includes honeysuckle, isatis root, scutellaria, indigo leaf, forsythia, platycodon, patchouli, and licorice. The reference standards are baicalin, ammonium glycyrrhizate, honeysuckle saponin B, and dipsacus saponin B. The detection method of this invention is simple, accurate, efficient, stable, precise, reproducible, convenient, and easy to master. It can effectively save energy and reduce analytical costs, while this technology is not disclosed in existing technologies.

The application provides a radix isatidis granule and a preparation method thereof, which is prepared from the following components in parts by weight: radix isatidis 700 parts, sucrose 170-190 parts and dextrin 50-70 parts. The content of active ingredient (R,S)-deoxyschizandrin, the total content of uridine, guanosine and adenosine, and the content of indirubin in the prepared radix isatidis granule are significantly improved, and the quality is stable after being accelerated for 6 months.

The invention discloses traditional Chinese medicine granules for treating verruca plana and a preparation method thereof. The traditional Chinese medicine granules comprise traditional Chinese medicine dry extract powder and auxiliary materials, the auxiliary materials comprise a diluent, a wetting agent and stevioside; wherein the traditional Chinese medicine dry extract powder is extracted from the following components in parts by weight: 60 parts of radix isatidis, 60 parts of semen coicis, 60 parts of herba portulacae, 30 parts of radix arnebiae seu lithospermi, 20 parts of semen persicae, 20 parts of flos carthami, 60 parts of herba equiseti hiemalis, 20 parts of rhizoma cyperi, 60 parts of nacre mother of pearl, 20 parts of honeycomb and 60 parts of raw oyster shell. The prepared granules are uniform in particle size and good in quality, the water content ranges from 1.59% to 1.79%, the forming rate ranges from 96.0% to 96.9%, the angle of repose ranges from 26.82 degrees to 29.99 degrees, the comprehensive performance score is high, and repeatability is good.

The application discloses a traditional Chinese medicine composition and a traditional Chinese medicine preparation for treating HPV infection. Specifically, the traditional Chinese medicine composition comprises the following components: radix codonopsis 15-45 parts, rhizoma atractylodis macrocephalae 7-25 parts, radix astragali 20-60 parts, radix pseudostellariae 7-25 parts, radix ophiopogonis 7-25 parts, radix rehmanniae 10-30 parts, coriolus 15-45 parts, ganoderma 15-45 parts, smilax riparia 20-60 parts, oldenlandia 15-35 parts, herba taraxaci 15-35 parts, morus 20-40 parts, coix 15-35 parts, gardenia 7-25 parts, salvia miltiorrhiza 10-30 parts, portulaca 15-45 parts, poria 10-30 parts, isatis root 10-30 parts, dendrobium 7-25 parts, schisandra 7-25 parts, licorice 10-20 parts, and cyperus 15-35 parts.

1. Name of the product in this design: Packaging bag (Astragalus and Astragalus Granules). 2. Purpose of this design: For packaging products. 3. The key design features of this product are the combination of its shape and pattern. 4. The image or photograph that best illustrates the design's key features: the front view. 5. This design is for a thin product, therefore the left view, right view, top view, and bottom view are omitted.

This application relates to the technical field of important veterinary preparations and discloses a preparation process for a compound traditional Chinese medicine granule preparation for veterinary use, including the following steps: (1) extracting dandelion, indigo leaf, isatis root, honeysuckle, licorice, patchouli, and gypsum with water to obtain an aqueous extract; adding scutellaria and phellodendron to the residue after water extraction and extracting with alcohol to obtain an alcohol extract; combining the extracts and concentrating to obtain a clear extract; (2) first mixing hydroxypropyl-β-cyclodextrin and sulfobutyl ether-β-cyclodextrin to obtain a mixed cyclodextrin solution, adding glycyrrhizic acid for pre-inclusion to form a glycyrrhizic acid-cyclodextrin pre-inclusion complex; then adding the obtained clear extract to continue inclusion to form an inclusion complex suspension; (3) mixing the inclusion complex suspension with pharmaceutical excipients, granulating and drying to obtain the final product. This application can improve the inclusion rate of baicalin and berberine and improve storage stability.

This invention discloses a compound Isatis root preparation and its preparation method, belonging to the field of traditional Chinese medicine preparation technology. It solves the problems of low extraction rate and high energy consumption of active ingredients in existing compound Isatis root preparations. The compound Isatis root preparation uses Isatis root and Isatis leaf as active ingredient raw materials, and includes steps such as swelling and cell wall disruption pretreatment, low-melting-point solvent-complex enzyme synergistic extraction, physical field cell wall disruption extraction, solid-liquid separation and concentration, and preparation molding. This invention can maximize the retention of heat-sensitive active ingredients, improve the dissolution of active ingredients in the compound Isatis root preparation, and greatly increase the extraction rate of active ingredients.

1. Name of the product in this design: Compressed atomizer (Isatis root). 2. Purpose of this design: For the atomization of liquid agents. 3. The key design feature of this product is its shape. 4. The image or photograph that best illustrates the design's key points: a 3D model.

The invention provides a traditional Chinese medicine composition for treating common diseases and symptoms of children and a preparation method and application thereof, and belongs to the technical field of traditional Chinese medicines. Active components of the traditional Chinese medicine composition comprise the following raw materials in parts by weight: 5-15 parts of honeysuckle; 5 to 15 parts of fructus forsythiae; 10 to 20 parts of radix isatidis; 5-15 parts of radix scutellariae; 3-10 parts of mint; 3-8 parts of liquorice; 5-15 parts of platycodon grandiflorum; 5-15 parts of herba schizonepetae; 5-15 parts of radix puerariae; 3-10 parts of radix bupleuri; 2-8 parts of cicada slough; 3 to 10 parts of fructus arctii; 5-15 parts of flos farfarae; 5-15 parts of ginger processed pinellia; 5 to 15 parts of oyster; 5-15 parts of bighead atractylodes rhizome; 5-15 parts of radix ophiopogonis; 5-15 parts of fructus cannabis; the compound preparation is optimized and adjusted according to the characteristics of children on the basis of a traditional Chinese medicine prescription, and has unique curative effects on common symptoms such as cold, cough, fever, anorexia, vomiting, diarrhea, constipation and development retardation of children. Vitamin C and vitamin E can also be added into the composition to enhance immunity. The traditional Chinese medicine composition is simple in preparation process, can be prepared into various dosage forms, is suitable for children of different ages, and is remarkable in curative effect, short in onset time, convenient to take, free of toxic and side effects, safe and effective.

This invention relates to the field of processing technology for Isatis indigotica leaves, specifically disclosing a processing device for Isatis indigotica leaves. The device includes a housing assembly comprising an outer shell, an end cap connected to the outer shell, and several feeding ports and water inlets on the end cap; a raw material placement assembly comprising a connecting pipe inside the outer shell communicating with the feeding ports and a placement box rotatably connected to the connecting pipe, the placement box including an upper gear disk, a lower gear disk, and a protective frame for placing raw materials disposed between the upper and lower gear disks, with gaps between adjacent protective frames; the connecting pipe is fixed to the end cap; and a water flow agitation assembly comprising a motor disposed at the bottom of the outer shell, the output shaft of the motor penetrating the outer shell and connected to a water flow stirring rod, the water flow stirring rod being equipped with a rotation component for the rotation of the raw material placement assembly. This device solves the problem that traditional cleaning devices, due to excessively vigorous cleaning, can damage the Isatis indigotica leaves, thus affecting their quality.

The present application relates to the technical field of traditional Chinese medicine, and particularly relates to a traditional Chinese medicine composition and application thereof in preparation of an antiviral medicine. The present application comprises honeysuckle, stevia leaf, wild chrysanthemum, isatis leaf and osmanthus, and the traditional Chinese medicine composition obtained thereby contains rich plant microRNA, MIR2911, thereby playing a good antiviral effect. Moreover, due to the negative effect of a flavoring agent on active ingredients in a product, the present application improves the prescription, and on the basis of avoiding the addition of a flavoring agent and a preservative, the product of the prescription obtains a good taste and can maintain stable quality for several months.

This invention relates to the field of traditional Chinese medicine processing technology, specifically disclosing a pretreatment method for traditional Chinese medicine including the following steps: Step S1, material selection: selecting mature Isatis root; Step S2, cleaning: using an integrated cleaning and cutting device to remove dirt and impurities from the surface of the Isatis root; Step S3, cutting: using the integrated cleaning and cutting device to cut the cleaned Isatis root into sections; the integrated cleaning and cutting device includes a housing, a cleaning component housed in the housing, a cutting component, and a conveying component that moves the Isatis root sequentially along the cleaning component and the cutting component; this solves the problem that in the traditional pretreatment process of Isatis root, the Isatis root is not cut, resulting in slow extraction of medicinal properties and waste of medicinal substances.

The present application relates to a kind of molecular marker combination of identifying isatis root rape celery and its application, the molecular marker combination is based on the T2T reference genome of newly assembled isatis indigotica Fort and the collinearity analysis of isatis indigotica Fort g chromosome and Brassica napus A, C subgenome development, can cover isatis indigotica Fort g chromosome key region (short arm, long arm and centromere), with strong specificity, comprehensive coverage, simple and fast operation, low cost and other advantages, can quickly and accurately identify the presence or absence of isatis indigotica Fort g chromosome in isatis root rape celery plant and its structural integrity in seedling stage, effectively solve the problems that existing identification method is complex, time efficiency is poor and accuracy is limited, to significantly improve and guarantee the production purity and efficiency of isatis root rape celery, and can be used for the authenticity identification of product, with wide application prospect and popularization value.

The invention provides a traditional Chinese medicine preparation for improving the cold treatment effect, namely a cold virus killing agent. The preparation method comprises the following steps: selecting several traditional Chinese medicinal materials with disinfection, sterilization and anti-inflammation functions, namely chrysanthemum indicum, asarum, coptis chinensis, scutellaria baicalensis, pepper, isatis root and liquorice, adding water, boiling twice, mixing the medicine liquids obtained by decocting twice, adding edible white vinegar accounting for 10% of the total medicine amount and an edible preservative accounting for 1% of the total medicine amount, and uniformly stirring. Viruses and bacteria in the nasal cavity can be directly killed; the main path of cold infection is the respiratory tract, the nasal cavity is the leading edge of virus infection, and then cold enters the whole body to cause morbidity; during treatment, the anti-cold drug is orally taken, the drug efficacy spreads all over the whole body, viruses can be quickly killed, but the drug efficacy of nasal mucosa and nasal liquid is difficult to enter, and at the moment, the nasal cavity becomes the base of the anti-cold drug from the leading edge of infection; at the moment, viruses in the nasal cavity can be killed by applying the cold virus killing agent to the nasal cavity, so that the treatment time is saved; the preparation is applied locally, and the efficacy of the preparation cannot reach the whole body, so that a patient must take at least one cold medicine when using the preparation, the cold virus killing agent can be independently used for treating rhinitis, but three medicines, namely magnolia flower, divaricate saposhnikovia root and solidago decurrens, for treating rhinitis must be added.

This invention provides a traditional Chinese medicine composition for treating herpes zoster, its preparation method, and its application, belonging to the field of traditional Chinese medicine technology. The active ingredients of the traditional Chinese medicine composition include the following two parts: (1) Traditional Chinese medicine active ingredients: by weight, prepared from the following traditional Chinese medicinal materials: 10-15 parts of Isatis root; 10-15 parts of Isatis leaf; 10-15 parts of Astragalus; 8-12 parts of Angelica sinensis; 8-12 parts of white peony root; 6-10 parts of Ligusticum chuanxiong; 5-8 parts of safflower; 5-8 parts of red peony root; 5-8 parts of Salvia miltiorrhiza; 4-7 parts of cicada slough; 4-7 parts of earthworm; 4-7 parts of Saposhnikovia divaricata; and 3-5 parts of licorice; (2) Western medicine active ingredients: composed of acyclovir and famciclovir in a weight ratio of 1:1-3. This invention is a traditional Chinese and Western medicine combined drug developed based on the traditional Chinese medicine treatment of herpes zoster and combined with modern pharmaceutical technology. It adopts bio-enzymatic hydrolysis technology and nano-level drug delivery system, which significantly improves the bioavailability and targeting of the drug. It has a unique therapeutic effect on shingles, and its advanced preparation process results in significant efficacy, short onset time, convenient administration, few adverse reactions, safety and effectiveness. It can also prevent and relieve postherpetic neuralgia, providing a new option for the treatment of shingles.